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22,705,234 | 2013-05-29 | 2012-12-03 | 1872-9800 | Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine | Collagen I confers gamma radiation resistance. | Azorin E, González-Martínez P R, Azorin J | eng | null | Journal Article | Collagen Type I | IM | 22705234, S0969-8043(12)00240-0, 10.1016/j.apradiso.2012.03.039 | The effect of collagen on the response of somatomammotroph tumor cells (GH3) to gamma, radiation therapy was studied in vitro. After incubating confluent GH3 cell monolayers in a serum-free, maintaining medium, either with or without collagen, the monolayers were irradiated with 137Cs, gamma radiation. Collagen reduces cell mortality via ERK1/2 activation, abolishing gamma radiation, cell death, and promotes cell invasion when acting in synergy with collagen and in association with the, MAPK/ERK1/2 signaling pathway activation. The presence of collagen in somatomammotroph tumors, confers resistance to radiation. | Animals, Cell Line, Tumor, Collagen Type I, Gamma Rays, MAP Kinase Signaling System, Neoplasm Invasiveness, Pituitary Neoplasms, Radiation Tolerance, Rats | null |
22,705,229 | 2012-11-07 | 2019-12-10 | 1879-0070 | Diagnostic microbiology and infectious disease | Epidemiologic surveillance to detect false-positive Mycobacterium tuberculosis cultures. | Lee Meng-Rui, Chung Kuei-Pin, Chen Wei-Ting, Huang Yu-Tsung, Lee Li-Na, Yu Chong-Jen, Teng Lee-Jene, Hsueh Po-Ren, Yang Pan-Chyr, Luh Kwen-Tay | eng | null | Evaluation Study, Journal Article | null | IM | 22705229, S0732-8893(12)00165-4, 10.1016/j.diagmicrobio.2012.04.014 | This study was aimed to investigate the ability of potential indices from epidemiologic surveillance to detect false-positive cultures of Mycobacterium tuberculosis (MTB). All clinical specimens for mycobacterial culture from April 1 to August 31, 2010, were reviewed. Single-positive cultures without relevant clinical and pathologic information were categorized as suspected false-positive cultures. Genotyping methods were used to confirm false-positive cultures. The performance of epidemiologic surveillance indices to detect potential false-positive cultures was evaluated. A total of 14,462 specimens were sent to the laboratory and 214 batches were processed in 107 work days (average 67.6 specimens per batch, ranging from 21 to 130 specimens per batch). Seventy-one single-positive cultures were identified, among which 5 cultures of multidrug-resistant MTB in 1 batch were false-positive, confirmed by genotyping methods. Epidemiologic surveillance with statistical process control charts for single-positive cultures per day showed good performance in epidemiologic surveillance. The false-positive rate was 38.5% in the 13 potential false-positive cultures according to the statistical process control chart for single-positive cultures per day. Although the incidence of tuberculous disease is high in Taiwan, clustering of multidrug-resistant MTB in 1 batch or clustering of single-positive cultures still suggested the occurrence of false-positive MTB cultures. Therefore, epidemiologic surveillance for the clustering of single-positive cultures with the statistical process control chart could be used to monitor the occurrence of false-positive results. | Aged, Aged, 80 and over, Bacteriological Techniques, Diagnostic Errors, Epidemiologic Methods, Female, Humans, Male, Middle Aged, Molecular Diagnostic Techniques, Mycobacterium tuberculosis, Taiwan, Tuberculosis, Young Adult | null |
22,705,235 | 2013-01-03 | 2017-11-16 | 1532-3102 | Placenta | Identification and localization of netrin-4 and neogenin in human first trimester and term placenta. | Dakouane-Giudicelli M, Duboucher C, Fortemps J, Salama S, Brulé A, Rozenberg P, de Mazancourt P | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Membrane Proteins, NTN4 protein, human, Nerve Growth Factors, Netrins, RNA, Messenger, neogenin | IM | 22705235, S0143-4004(12)00210-X, 10.1016/j.placenta.2012.05.011 | We describe here for the first time the characterization of family member of netrins, netrin-4 and its receptor neogenin, during the development of the placenta. By using western blots and RT-PCR, we demonstrated the presence of netrin-4 and its receptor neogenin protein as well as their transcripts. Using immunohistochemistry, we studied the distribution of netrin-4 and neogenin in both the first trimester and term placenta. We observed staining of netrin-4 in villous and extravillous cytotrophoblasts, syncytiotrophoblast, and endothelial cells whereas staining in stromal cells was faint. In decidua, we observed netrin-4 labelling in glandular epithelial cells, perivascular decidualized cells, and endothelial cells. However, neogenin was absent in villous and extravillous cytotrophoblasts and was expressed only on syncytiotrophoblast and placental stromal cells in the first trimester and at term placenta. The pattern of distribution suggests that a functional netrin-4-neogenin pathway might be restricted to syncytiotrophoblasts, mesenchymal cells, and villous endothelial cells. This pathway function might vary with its localization in the placenta. It is possibly involved in angiogenesis, morphogenesis, and differentiation. | Decidua, Endothelial Cells, Female, Gene Expression, Humans, Immunohistochemistry, Labor, Obstetric, Membrane Proteins, Mesoderm, Nerve Growth Factors, Netrins, Parturition, Placenta, Pregnancy, Pregnancy Trimester, First, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Stromal Cells, Trophoblasts | null |
22,705,236 | 2012-11-26 | 2017-11-16 | 1525-2191 | The American journal of pathology | CDKN1C/P57 is regulated by the Notch target gene Hes1 and induces senescence in human hepatocellular carcinoma. | Giovannini Catia, Gramantieri Laura, Minguzzi Manuela, Fornari Francesca, Chieco Pasquale, Grazi Gian Luca, Bolondi Luigi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Basic Helix-Loop-Helix Transcription Factors, CDKN1C protein, human, Cyclin-Dependent Kinase Inhibitor p57, Homeodomain Proteins, NOTCH1 protein, human, NOTCH3 protein, human, Receptor, Notch1, Receptor, Notch3, Receptors, Notch, Transcription Factor HES-1, HES1 protein, human | IM | 22705236, S0002-9440(12)00358-6, 10.1016/j.ajpath.2012.04.019 | CDKN1C/P57 is a cyclin-dependent kinase inhibitor implicated in different human cancers, including hepatocellular carcinoma (HCC); however, little is known regarding the role of CDKN1C/P57 and its regulation in HCC. In this study, we show that the down-regulation of Notch1 and Notch3 in two HCC cell lines resulted in Hes1 down-regulation, CDKN1C/P57 up-regulation, and reduced cell growth. In line with these data, we report that CDKN1C/P57 is a target of transcriptional repression by the Notch effector, Hes1. We found that the up-regulation of CDKN1C/P57 by cDNA transfection decreased tumor growth, as determined by growth curve, flow cytometry analysis, and cyclin D1 down-regulation, without affecting the apoptosis machinery. Indeed, the expression of Bax, Noxa, PUMA, BNIP(3), and cleaved caspase-3 was not affected by CDKN1C/P57 induction. Morphologically CDKN1C/p57-induced HCC cells became flat and lengthened in shape, accumulated the senescence-associated β-galactosidase marker, and increased P16 protein expression. Evaluation of senescence in cells depleted both for Hes1 and CDKN1C/P57 revealed that the senescent state really depends on the accumulation of CDKN1C/p57. Finally, we validated our in vitro results in primary HCCs, showing that Hes1 protein expression inversely correlates with CDKN1C/P57 mRNA levels. In addition, reduced Hes1 protein expression is accompanied by a shorter time to recurrence after curative resection, suggesting that Hes1 may represent a biomarker for prediction of patients with poor prognosis. | Aged, Apoptosis, Basic Helix-Loop-Helix Transcription Factors, Carcinoma, Hepatocellular, Cell Cycle, Cell Line, Tumor, Cell Proliferation, Cellular Senescence, Chromatin Immunoprecipitation, Cyclin-Dependent Kinase Inhibitor p57, Female, Fluorescent Antibody Technique, Gene Expression Regulation, Neoplastic, Gene Silencing, Homeodomain Proteins, Humans, Liver Neoplasms, Male, Middle Aged, Receptor, Notch1, Receptor, Notch3, Receptors, Notch, Recurrence, Signal Transduction, Time Factors, Transcription Factor HES-1 | null |
22,705,239 | 2013-02-04 | 2025-01-03 | 1532-821X | Archives of physical medicine and rehabilitation | Mauritius calling: medical care and neurorehabilitation needs in an oceanic idyll. | Soopramanien Anba | eng | null | Journal Article | null | IM | 22705239, S0003-9993(12)00402-9, 10.1016/j.apmr.2012.05.023 | The tropical island of Mauritius, located off the coast of Southern Africa, has greatly improved its health care system, especially for frontline services and procedures such as cardiac surgery. But the post-acute neurorehabilitation care is still problematic, much to the detriment of disabled patients, their families, and Mauritian society overall. Comparisons with neurorehabilitation care in the United Kingdom suggest the scale of the problem in terms of uncoordinated medical teams, limited follow-ups, lack of expertise, and cultural stigma. This article assesses the needs of the neurologic rehabilitation segment in Mauritian health care and submits a set of policy recommendations addressing what medical professionals, hospitals, government officials, and other organizations can do to improve the neurologic rehabilitation infrastructure for Mauritian patients. | Brain Injuries, Delivery of Health Care, Persons with Disabilities, Health Services Needs and Demand, Humans, Mauritius, Rehabilitation Centers | null |
22,705,240 | 2013-01-14 | 2012-10-29 | 1532-821X | Archives of physical medicine and rehabilitation | Health outcomes associated with military deployment: mild traumatic brain injury, blast, trauma, and combat associations in the Florida National Guard. | Vanderploeg Rodney D, Belanger Heather G, Horner Ronnie D, Spehar Andrea M, Powell-Cope Gail, Luther Stephen L, Scott Steven G | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22705240, S0003-9993(12)00403-0, 10.1016/j.apmr.2012.05.024 | To determine the association between specific military deployment experiences and immediate and longer-term physical and mental health effects, as well as examine the effects of multiple deployment-related traumatic brain injuries (TBIs) on health outcomes. | Anxiety, Blast Injuries, Brain Injuries, Cross-Sectional Studies, Depression, Female, Florida, Health Status, Humans, Internet, Male, Mental Health, Military Personnel, Self Report, Socioeconomic Factors, Stress Disorders, Post-Traumatic, Time Factors, United States | null |
22,705,237 | 2013-01-14 | 2022-03-18 | 1532-821X | Archives of physical medicine and rehabilitation | Effectiveness of telephone counseling in managing psychological outcomes after spinal cord injury: a preliminary study. | Dorstyn Diana, Mathias Jane, Denson Linley, Robertson Marie | eng | null | Journal Article, Randomized Controlled Trial | null | IM | 22705237, S0003-9993(12)00405-4, 10.1016/j.apmr.2012.06.002 | To determine whether an individualized counseling intervention delivered by telephone-telecounseling-feasibly improves the emotional adjustment of adults with a newly acquired spinal cord injury (SCI). | Adaptation, Psychological, Aged, Anxiety, Counseling, Depression, Female, Humans, Male, Middle Aged, Socioeconomic Factors, Spinal Cord Injuries, Telephone | null |
22,705,241 | 2012-12-03 | 2012-07-16 | 1878-5506 | Sleep medicine | Healthcare systems never sleep: are medical residents today the patients of tomorrow? | Schiöth Helgi Birgir, Brooks Samantha Jane, Benedict Christian | eng | null | Letter, Research Support, Non-U.S. Gov't | null | IM | 22705241, S1389-9457(12)00214-6, 10.1016/j.sleep.2012.05.001 | null | Adult, Female, Humans, Internship and Residency, Male, Middle Aged, Sleep Deprivation | null |
22,705,238 | 2013-02-04 | 2012-11-26 | 1532-821X | Archives of physical medicine and rehabilitation | Responsiveness of the motor function measure in neuromuscular diseases. | Vuillerot Carole, Payan Christine, Girardot Françoise, Fermanian Jacques, Iwaz Jean, Bérard Carole, Ecochard René | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705238, S0003-9993(12)00404-2, 10.1016/j.apmr.2012.05.025 | To study the responsiveness (sensitivity to change) of the Motor Function Measure (MFM) in detecting change in neuromuscular disease patients with the intent of using this measure in future clinical trials. | Adolescent, Adult, Aged, Child, Disability Evaluation, Female, Humans, Male, Middle Aged, Mobility Limitation, Motor Skills, Muscular Dystrophy, Duchenne, Neuromuscular Diseases, Perception, Physical Therapy Modalities, Prospective Studies, Young Adult | null |
22,705,242 | 2012-12-03 | 2015-11-19 | 1878-5506 | Sleep medicine | Sleep complaints among adolescent outpatients with major depressive disorder. | Urrila Anna S, Karlsson Linnea, Kiviruusu Olli, Pelkonen Mirjami, Strandholm Thea, Marttunen Mauri | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705242, S1389-9457(12)00194-3, 10.1016/j.sleep.2012.04.012 | The objective of our study was to examine the prevalence rates of different sleep complaints among adolescent outpatients with major depressive disorder (MDD). Further, we examined whether depressed adolescents with and without different sleep disturbances differ in terms of severity of depression, the presence of comorbid psychiatric disorders, and the symptom profile of depression. | Adolescent, Depressive Disorder, Major, Disorders of Excessive Somnolence, Female, Finland, Humans, Male, Prevalence, Psychiatric Status Rating Scales, Sex Factors, Sleep Disorders, Circadian Rhythm, Sleep Initiation and Maintenance Disorders, Sleep Wake Disorders, Surveys and Questionnaires, Young Adult | null |
22,705,243 | 2012-12-03 | 2012-07-16 | 1878-5506 | Sleep medicine | REM rebound and CPAP compliance. | Koo Brian B, Wiggins Roger, Molina Carol | eng | null | Journal Article | null | IM | 22705243, S1389-9457(12)00186-4, 10.1016/j.sleep.2012.03.019 | The objective of this study was establish if rapid-eye-movement (REM) rebound on first exposure to continuous positive airway pressure (CPAP) is associated with CPAP compliance. A rebound or drastic increase in REM sleep in response to initial CPAP exposure is associated with improvement in the subjective quality of sleep. We wished to determine if REM rebound was also associated with increased CPAP compliance. | Continuous Positive Airway Pressure, Humans, Male, Middle Aged, Patient Compliance, Polysomnography, Severity of Illness Index, Sleep Apnea, Obstructive, Sleep, REM, Time Factors | null |
22,705,245 | 2012-12-03 | 2022-04-08 | 1878-5506 | Sleep medicine | Symptoms of sleep apnea syndrome: high prevalence and underdiagnosis in the French population. | Fuhrman Claire, Fleury Bernard, Nguyên Xuân-Lan, Delmas Marie-Christine | eng | null | Journal Article | null | IM | 22705245, S1389-9457(12)00187-6, 10.1016/j.sleep.2012.04.005 | To determine the prevalence of symptoms evocative of obstructive sleep apnea (SE-OSA) and the magnitude of obstructive sleep apnea (OSA) underdiagnosis. | Adolescent, Adult, Cross-Sectional Studies, Diagnostic Errors, Female, France, Humans, Hypertension, Interviews as Topic, Logistic Models, Male, Middle Aged, Obesity, Prevalence, Sleep Apnea, Obstructive, Surveys and Questionnaires, Young Adult | null |
22,705,244 | 2012-12-03 | 2015-11-19 | 1878-5506 | Sleep medicine | Validation of the Japanese version of the REM sleep behavior disorder questionnaire (RBDQ-JP). | Sasai Taeko, Matsuura Masato, Wing Yun Kwok, Inoue Yuichi | eng | null | Journal Article | null | IM | 22705244, S1389-9457(12)00193-1, 10.1016/j.sleep.2012.04.011 | The rapid eye movement (REM) sleep behavior disorder (RBD) questionnaire (RBDQ)-Hong Kong was the first tool developed for quantifying the severity of RBD. This study was conducted to validate the Japanese version of the questionnaire and to investigate its reliability, validity, and responsiveness. | Aged, Factor Analysis, Statistical, Female, Humans, Japan, Male, Middle Aged, Polysomnography, REM Sleep Behavior Disorder, Reproducibility of Results, Sensitivity and Specificity, Surveys and Questionnaires, Translating | null |
22,705,246 | 2012-12-03 | 2015-11-19 | 1878-5506 | Sleep medicine | Prevalence of sleep disturbances, disorders, and problems following traumatic brain injury: a meta-analysis. | Mathias J L, Alvaro P K | eng | null | Journal Article, Meta-Analysis | null | IM | 22705246, S1389-9457(12)00188-8, 10.1016/j.sleep.2012.04.006 | Sleep is often disrupted following a traumatic brain injury (TBI), which may compromise recovery and quality of life. Prevalence rates vary widely, reflecting differences in the criteria and measures that are used to assess sleep, as well as sample differences. This meta-analysis examined the prevalence of general and specific, and formally and informally diagnosed, sleep disturbances following TBI in order to establish the nature and extent of these sequelae and their potential impact on recovery. | Adult, Brain Injuries, Disorders of Excessive Somnolence, Female, Humans, Injury Severity Score, Male, Narcolepsy, Nocturnal Myoclonus Syndrome, Prevalence, Sleep Apnea, Obstructive, Sleep Wake Disorders | null |
22,705,248 | 2013-04-30 | 2020-12-22 | 1873-4995 | Journal of controlled release : official journal of the Controlled Release Society | Finding the optimal balance: challenges of improving conventional cancer chemotherapy using suitable combinations with nano-sized drug delivery systems. | Kratz Felix, Warnecke André | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Albumins, Liposomes, Prodrugs | IM | 22705248, S0168-3659(12)00458-0, 10.1016/j.jconrel.2012.05.045 | Anticancer drugs as well as nano-sized drug delivery systems face many barriers that hinder penetration deeply and evenly into solid tumors: a chaotic, tortuous vascular compartment resulting in tumor tissue distant from microvessels, a heterogeneous blood flow distribution with a concomitant defective microcirculatory exchange process, and a high interstitial fluid pressure. Furthermore, a resulting hostile tumor microenvironment characterized by hypoxia and/or extracellular acidosis can reduce the efficacy of anticancer drugs and confer drug resistance. Conversely, the enhanced permeation and retention effect has become the gold standard for developing macromolecular prodrugs and nano-sized drug delivery systems. Preclinically, there are meanwhile numerous in vivo proof-of-concepts that demonstrate not only a better tolerability of nano-sized drug delivery systems but also of enhanced antitumor efficacy compared to the conventional clinical standard. When faced with such a complex and heterogeneous disease as cancer in humans, it is more likely that a tailor-made combination of different therapeutic strategies will achieve the best results. In this respect, combining low-molecular weight cytostatic drugs with nano-sized drug delivery systems appears to be a natural choice for combination therapy that aims at distributing anticancer drugs at higher concentrations in the tumor in a more even manner. To date, such drug delivery approaches have been inadequately explored. In this review, we summarize the state-of-the-art of combination approaches with liposomal doxorubicin (Doxil™), the paclitaxel-albumin nanoparticle (Abraxane™) and the albumin-binding doxorubicin prodrug DOXO-EMCH (INNO-206), and discuss the insights obtained and perspectives for further research in this intriguing and promising field of drug delivery research. | Albumins, Animals, Drug Delivery Systems, Drug Therapy, Combination, Humans, Liposomes, Nanoparticles, Neoplasms, Prodrugs | null |
22,705,247 | 2013-05-17 | 2022-03-09 | 1878-5506 | Sleep medicine | Sleep disturbances and risk of frailty and mortality in older men. | Ensrud Kristine E, Blackwell Terri L, Ancoli-Israel Sonia, Redline Susan, Cawthon Peggy M, Paudel Misti L, Dam Thuy-Tien L, Stone Katie L | eng | U01 AR45632 (NIAMS NIH HHS, United States); R01 AG008415 (NIA NIH HHS, United States); R01 HL070848 (NHLBI NIH HHS, United States); R01 HL071194 (NHLBI NIH HHS, United States); R01 HL070847 (NHLBI NIH HHS, United States); U01 AR45647 (NIAMS NIH HHS, United States); R01 HL070842 (NHLBI NIH HHS, United States); U01 AR45614 (NIAMS NIH HHS, United States); U01 AR045654 (NIAMS NIH HHS, United States); U01 AG042168 (NIA NIH HHS, United States); U01 AR45583 (NIAMS NIH HHS, United States); R01 HL070839 (NHLBI NIH HHS, United States); R01 HL070837 (NHLBI NIH HHS, United States); U01 AR045614 (NIAMS NIH HHS, United States); U01 AR45654 (NIAMS NIH HHS, United States); U01 AR045583 (NIAMS NIH HHS, United States); R01 HL070841 (NHLBI NIH HHS, United States); U01 AG042140 (NIA NIH HHS, United States); R01 HL070838 (NHLBI NIH HHS, United States); U01 AR045647 (NIAMS NIH HHS, United States); U01 AR45580 (NIAMS NIH HHS, United States); U01 AG027810 (NIA NIH HHS, United States); AG08415 (NIA NIH HHS, United States); UL1 RR024140 (NCRR NIH HHS, United States); U01 AR045580 (NIAMS NIH HHS, United States); U01 AG18197 (NIA NIH HHS, United States); U01 AG018197 (NIA NIH HHS, United States); U01 AR066160 (NIAMS NIH HHS, United States); U01 AR045632 (NIAMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705247, S1389-9457(12)00192-X, 10.1016/j.sleep.2012.04.010, PMC3449012, NIHMS376424, 18948557, 16085466, 19220560, 11300121, 9493915, 18482297, 19465743, 12554816, 19245414, 16282178, 18819173, 16408420, 17767677, 18714780, 2748771, 20870457, 10682939, 18759758, 19688045, 1519015, 19793356, 12749557, 19246707, 19793160, 9048689, 18413429, 17969465, 18714778, 11966340, 16084776, 11253156, 17661960, 19238807, 10726435, 3611032 | To test the hypothesis that non-frail older men with poorer sleep at baseline are at increased risk of frailty and death at follow-up. | Actigraphy, Aged, Disorders of Excessive Somnolence, Frail Elderly, Humans, Male, Polysomnography, Prospective Studies, Risk Factors, Sleep Apnea Syndromes, Sleep Wake Disorders | null |
22,705,250 | 2013-01-04 | 2022-01-29 | 1873-5134 | Patient education and counseling | Public perceptions and preferences for CT colonography or colonoscopy in colorectal cancer screening. | Ghanouni Alex, Smith Samuel G, Halligan Steve, Plumb Andrew, Boone Darren, Magee Molly Sweeney, Wardle Jane, von Wagner Christian | eng | 14134 (Cancer Research UK, United Kingdom); RP-PG-0407-10338 (Department of Health, United Kingdom) | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | null | null | 22705250, S0738-3991(12)00225-X, 10.1016/j.pec.2012.05.007 | To examine public perceptions of and preferences for colonoscopy vs. CT colonography (CTC) as technologies for colorectal cancer (CRC) screening. | Colonography, Computed Tomographic, Colonoscopy, Colorectal Neoplasms, Community Participation, Early Detection of Cancer, Female, Focus Groups, Health Knowledge, Attitudes, Practice, Humans, Male, Mass Screening, Middle Aged, Patient Preference, Perception, Sensitivity and Specificity, Surveys and Questionnaires | null |
22,705,249 | 2013-06-11 | 2019-12-10 | 1532-7361 | Surgery | Resection of peritoneal metastases in patients with hepatocellular carcinoma. | Hashimoto Masaji, Sasaki Kazunari, Moriyama Jin, Matsuda Masamichi, Watanabe Goro | eng | null | Evaluation Study, Journal Article | null | IM | 22705249, S0039-6060(12)00157-2, 10.1016/j.surg.2012.03.031 | Peritoneal metastases from hepatocellular carcinoma are common; they are found in as many as 18% of autopsy cases. Effective treatment for peritoneal metastases, however, has not yet been established. | Aged, Carcinoma, Hepatocellular, Female, Follow-Up Studies, Humans, Liver Neoplasms, Male, Middle Aged, Palliative Care, Peritoneal Neoplasms, Peritoneum, Survival Rate, Treatment Outcome | null |
22,705,254 | 2013-04-11 | 2023-12-13 | 1618-1328 | Journal of plant physiology | Expression of Arabidopsis plastidial phosphoglucomutase in tobacco stimulates photosynthetic carbon flow into starch synthesis. | Uematsu Kimio, Suzuki Nobuaki, Iwamae Tomoko, Inui Masayuki, Yukawa Hideaki | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Starch, Phosphoglucomutase | IM | 22705254, S0176-1617(12)00214-3, 10.1016/j.jplph.2012.05.008 | Phosphoglucomutase (PGM, EC 2.7.5.1) is one of the enzymes constituting the carbohydrate synthesis pathway in higher plants. It catalyzes the reversible conversion of glucose 6-phosphate (Glc6P) to glucose 1-phosphate (Glc1P). Previously, metabolic turnover analysis using (13)CO(2) in tobacco leaves demonstrated that conversion of Glc6P to Glc1P may limit carbon flow into carbohydrate synthesis. In order to assess the effects of PGM, Arabidopsis thaliana cytosolic or plastidial PGM was expressed under the control of cauliflower mosaic virus 35S promoter in tobacco plants (Nicotiana tabacum cv. Xanthi) and phenotypic analysis was performed. The transgenic plants expressing Arabidopsis plastidial PGM showed 3.5-8.2-fold higher PGM activity than that of wild-type, and leaf starch and sucrose contents increased 2.3-3.2-fold and 1.3-1.4-fold, respectively over wild-type levels. In vivo(13)C-labeling experiments indicated that photosynthetically fixed carbon in the transgenic plants could be converted faster to Glc1P and adenosine 5'-diphosphate glucose than in wild-type, suggesting that elevation of plastidial PGM activity should accelerate conversion of Glc6P to Glc1P in chloroplasts and increase carbon flow into starch. On the other hand, transgenic plants expressing Arabidopsis cytosolic PGM showed a 2.1-3.4-fold increase in PGM activity over wild-type and a decrease of leaf starch content, but no change in sucrose content. These results suggest that plastidial PGM limits photosynthetic carbon flow into starch. | Arabidopsis, Biological Transport, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant, Phosphoglucomutase, Photosynthesis, Plant Leaves, Plants, Genetically Modified, Plastids, Starch, Nicotiana | null |
22,705,251 | 2013-03-08 | 2019-12-10 | 1879-0720 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | A multifunctional 5-aminolevulinic acid derivative induces erythroid differentiation of K562 human erythroleukemic cells. | Berkovitch-Luria G, Yakobovitch S, Weitman M, Nudelman A, Rozic G, Rephaeli A, Malik Z | eng | null | Journal Article | AlaAcBu, Glycophorins, Hemoglobins, Levulinic Acids, Prodrugs, RNA, Messenger, alpha-Globins, Butyric Acid, Erythropoietin, Heme, Aminolevulinic Acid, Hydroxymethylbilane Synthase, Ferrochelatase | IM | 22705251, S0928-0987(12)00244-8, 10.1016/j.ejps.2012.05.017 | Anemia is a major clinical symptom of a wide variety of pathological conditions a common related to reduced erythropoiesis. Whereas erythropoietin treatment showed an improvement in the patients' condition, it revealed increased risks of thromboembolic and cardiovascular events. Herein we describe stimulation of erythropoiesis by the multifunctional 1-(butyryloxy)ethyl-5-amino-4-oxopentanoate, (AlaAcBu), a 5-aminolevulinic-acid (ALA) derivative, which undergoes metabolic hydrolysis yielding two erythroid differentiation inducers, ALA and butyric acid (BA), each acting through a different mechanism. ALA, the first precursor in the heme biosynthesis, accelerates heme synthesis and BA, a histone deacetylase inhibitor (HDACI) that activates the transcription of globin mRNA. Our results show that the AlaAcBu mutual prodrug is a potent chemical differentiation inducer of K562 human erythroleukemia cells manifested by augmentation of heme and globin synthesis and assembly of hemoglobin. Exposure of K-562 cells to AlaAcBu resulted in an increase in heme synthesis and globin expression. Stimulation of the heme pathway was evident by the over-expression of porphobilinogen deaminase (PBGD) and ferrochelatase. AlaAcBu promoted cellular erythroid differentiation depicted by the expression of the marker glycophorin A and cellular maturation characterized by cytoplasm hemoglobinization, polar arrangement of mitochondria and a developed central vacuolar system preceding nuclear extrusion. The ability of AlaAcBu to promote differentiation along the erythroid lineage and to dramatically induce hemoglobin synthesis presented in this report. | Aminolevulinic Acid, Butyric Acid, Cell Differentiation, Cell Proliferation, Erythroid Cells, Erythropoiesis, Erythropoietin, Ferrochelatase, Glycophorins, Heme, Hemoglobins, Humans, Hydrolysis, Hydroxymethylbilane Synthase, K562 Cells, Leukemia, Erythroblastic, Acute, Levulinic Acids, Prodrugs, RNA, Messenger, alpha-Globins | null |
22,705,255 | 2012-11-19 | 2017-11-16 | 1879-1611 | Journal of insect physiology | Population dependent effects of photoperiod on diapause related physiological traits in an invasive beetle (Leptinotarsa decemlineata). | Lehmann Philipp, Lyytinen Anne, Sinisalo Tuula, Lindström Leena | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705255, S0022-1910(12)00135-7, 10.1016/j.jinsphys.2012.06.003 | Organisms undergoing latitudinal range expansion face a change in the photoperiod which can lead to a mismatch between the timing of seasonal changes in physiological and life history traits with seasonal environmental changes. This mismatch can lead to lowered survival, for example, due to unsynchronized diapause timing. Successful range expansion even in recent introductions requires that organisms which use the photoperiod for seasonal predictions should show interpopulational differences in photoperiodic responses at different latitudes, as the photoperiod is a function of latitude. We investigated among population differences in photoperiodic responses of life history and physiological traits linked to diapause in the invasive beetle Leptinotarsa decemlineata. Beetles from a northern marginal and a southern European population were reared under short day (12:12L:D) and long day (18:6L:D) photoperiods. Both populations reacted similarly to the short day photoperiod. Their abdominal total lipid content increased and water content decreased which suggests that the beetles prepared for diapause. This was also indicated by low mortality during diapause. In the long day photoperiod large interpopulational differences were found, the southern population ceased lipid accumulation after 5 days, while the northern population continued lipid accumulation as beetles in the short day photoperiod. This indicates that the northern population has a longer critical photoperiod than the southern one. Abdominal total lipid stores in 10 day old beetles were shown to be predominantly composed of neutral lipids (85%), most likely representing storage triacylglycerols. Fatty acid profiles of both the neutral lipids and the phospholipids showed large shifts during the first 10 day of adult life, predominantly in the fractions of 18:0, 18:1ω9, 18:2ω6 and 18:3ω3. Although the degree of unsaturation increased with age, it was not higher in diapausing than non-diapausing beetles. This indicates that this species does not increase diapause related cold tolerance via homeoviscous adaptation, and might have developed other means to cope with suboptimal temperatures, such as behavioral adaptations. | Animals, Coleoptera, Female, Light, Lipid Metabolism, Male, Metamorphosis, Biological, Photoperiod, Seasons | null |
22,705,253 | 2012-12-07 | 2020-09-30 | 1618-1328 | Journal of plant physiology | Over-expression of GsZFP1, an ABA-responsive C2H2-type zinc finger protein lacking a QALGGH motif, reduces ABA sensitivity and decreases stomata size. | Luo Xiao, Cui Na, Zhu Yanming, Cao Lei, Zhai Hong, Cai Hua, Ji Wei, Wang Xuedong, Zhu Dan, Li Yong, Bai Xi | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Kruppel-Like Transcription Factors, Plant Growth Regulators, Plant Proteins, Abscisic Acid | IM | 22705253, S0176-1617(12)00194-0, 10.1016/j.jplph.2012.03.019 | A cDNA of the gene GsZFP1 was cloned from Glycine soja. GsZFP1 encodes a protein with one C2H2-type zinc finger motif. The QALGGH motif, which exists in most plant C2H2-type zinc finger proteins (ZFPs), does not exist in GsZFP1. Real-time RT-PCR revealed that GsZFP1 expression was significantly up-regulated by exogenous ABA, both in leaves and roots. Over-expression of this gene, in Arabidopsis thaliana, resulted in a reduced sensitivity to ABA during seed germination and seedling growth. Transcript levels of some stress and ABA marker genes, including RD29A, RD22, NCED3, COR47, COR15A and KIN1 were increased, in the GsZFP1 over-expression lines, when plants were treated with exogenous ABA. We further studied the effects of GsZFP1 over-expression on the regulation of genes involved in ABA signaling. Negative ABA signaling regulators, such as ABI1 and ABI2, were up-regulated in over-expression lines, while positive ABA signaling regulators, such as ABF4, ABI5, GTG1, GTG2, PYR1/RCAR11, PYL2/RCAR13, SnRK2.2 and SnRK2.3, were down-regulated, in comparison to wild type plants. GsZFP1 over-expression lines also exhibited small stomata, impairment of ABA-induced stomata closure. The data presented, herein, suggests that GsZFP1 plays a crucial role in ABA signaling in A. thaliana, GsZFP1 may be a promising gene for negative regulating ABA signaling. Our findings broaden our understanding of this C2H2 ZFP subtype's function, and add to the body of evidence that has been developed in earlier studies. | Abscisic Acid, Arabidopsis, Gene Expression Regulation, Plant, Genetic Variation, Genotype, Germination, Kruppel-Like Transcription Factors, Plant Growth Regulators, Plant Leaves, Plant Proteins, Plant Roots, Plant Stomata, Seedlings, Signal Transduction, Up-Regulation, Zinc Fingers | null |
22,705,252 | 2013-01-07 | 2021-10-21 | 1879-3134 | Mathematical biosciences | Longitudinal multistage model for lung cancer incidence, mortality, and CT detected indolent and aggressive cancers. | Hazelton William D, Goodman Gary, Rom William N, Tockman Melvyn, Thornquist Mark, Moolgavkar Suresh, Weissfeld Joel L, Feng Ziding | eng | UO1 CA084986 (NCI NIH HHS, United States); UO1 CA097415 (NCI NIH HHS, United States); P50 CA090440 (NCI NIH HHS, United States); U01 CA63673 (NCI NIH HHS, United States); U01 CA152956 (NCI NIH HHS, United States); UO1 CA86368 (NCI NIH HHS, United States); 5U01CA086137 (NCI NIH HHS, United States); U01 CA063673 (NCI NIH HHS, United States); UO1 CA152956 (NCI NIH HHS, United States); U01 CA097415 (NCI NIH HHS, United States); R01 CA107028 (NCI NIH HHS, United States); U01 CA086368 (NCI NIH HHS, United States); P50 CA90440 (NCI NIH HHS, United States); U01CA097415 (NCI NIH HHS, United States); U01 CA084986 (NCI NIH HHS, United States); U01 CA086137 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22705252, S0025-5564(12)00124-1, 10.1016/j.mbs.2012.05.008, PMC3412888, NIHMS387335, 17302181, 19318668, 11205667, 21254049, 8003339, 1958856, 20058155, 16137718, 18458247, 19262443, 18364449, 435370, 14871239, 21714641, 17873156, 20530126, 11139308, 6733223, 18374369, 9232020, 12644540, 12673242, 2195604, 18058248, 20213704, 1828119, 18653771, 16268939, 9620938, 18635890, 8608967, 18816496, 21045183, 15894668, 18317061, 6941039, 16087198, 19318993, 16452578, 19293258, 12210622, 17341709, 19581354, 21454908, 19629730, 10228131 | It is currently not known whether most lung cancers detected by computerized tomography (CT) screening are aggressive and likely to be fatal if left untreated, or if a sizable fraction are indolent and unlikely to cause death during the natural lifetime of the individual. We developed a longitudinal biologically-based model of the relationship between individual smoking histories and the probability for lung cancer incidence, CT screen detection, lung cancer mortality, and other-cause mortality. The longitudinal model relates these different outcomes to an underlying lung cancer disease pathway and an effective other-cause mortality pathway, which are both influenced by the individual smoking history. The longitudinal analysis provides additional information over that available if these outcomes were analyzed separately, including testing if the number of CT detected and histologically-confirmed lung cancers is consistent with the expected number of lung cancers "in the pipeline". We assume indolent nodules undergo Gompertz growth and are detectable by CT, but do not grow large enough to contribute significantly to symptom-based lung cancer incidence or mortality. Likelihood-based model calibration was done jointly to data from 6878 heavy smokers without asbestos exposure in the control (placebo) arm of the Carotene and Retinol Efficacy Trial (CARET); and to 3,642 heavy smokers with comparable smoking histories in the Pittsburgh Lung Screening Study (PLuSS), a single-arm prospective trial of low-dose spiral CT screening for diagnosis of lung cancer. Model calibration was checked using data from two other single-arm prospective CT screening trials, the New York University Lung Cancer Biomarker Center (NYU) (n=1,021), and Moffitt Cancer Center (Moffitt) cohorts (n=677). In the PLuSS cohort, we estimate that at the end of year 2, after the baseline and first annual CT exam, that 33.0 (26.9, 36.9)% of diagnosed lung cancers among females and 7.0 (4.9,11.7)% among males were overdiagnosed due to being indolent cancers. At the end of the PLuSS study, with maximum follow-up of 5.8 years, we estimate that due to early detection by CT and limited follow-up, an additional 2.2 (2.0,2.4)% of all diagnosed cancers among females and 7.1 (6.7,8.0)% among males would not have been diagnosed in the absence of CT screening. We also find a higher apparent cure rate for lung cancer among CARET females than males, consistent with the larger indolent fraction of CT detected and histologically confirmed lung cancers among PLuSS females. This suggests that there are significant gender differences in the aggressiveness of lung cancer. Females may have an inherently higher proportion of indolent lung cancers than males, or aggressive lung cancers may be brought into check by the immune system more frequently among females than males. | Female, Humans, Incidence, Longitudinal Studies, Lung Neoplasms, Male, Mass Screening, Middle Aged, Models, Statistical, New York, Pennsylvania, Sex Factors, Smoking, Tomography, X-Ray Computed | null |
22,705,256 | 2012-11-19 | 2020-12-30 | 1879-1611 | Journal of insect physiology | Phenotypic impacts of PBAN RNA interference in an ant, Solenopsis invicta, and a moth, Helicoverpa zea. | Choi Man-Yeon, Vander Meer Robert K, Coy Monique, Scharf Michael E | eng | null | Journal Article | Insect Hormones, Insect Proteins, Neuropeptides, Sex Attractants, pheromone biosynthesis activating neuropeptide, Helicoverpa zea | IM | 22705256, S0022-1910(12)00137-0, 10.1016/j.jinsphys.2012.06.005 | Insect neuropeptide hormones represent more than 90% of all insect hormones. The PBAN/pyrokinin family is a major group of insect neuropeptides, and they are expected to be found from all insect groups. These species-specific neuropeptides have been shown to have a variety of functions from embryo to adult. PBAN is well understood in moth species relative to sex pheromone biosynthesis, but other potential functions are yet to be determined. Recently, we focused on defining the PBAN gene and peptides in fire ants in preparation for an investigation of their function(s). RNA interference (RNAi) technology is a convenient tool to investigate unknown physiological functions in insects, and it is now an emerging method for development of novel biologically-based control agents as alternatives to insecticides. This could be a paradigm shift that will avoid many problems associated with conventional chemical insecticides. In this study, we selected the PBAN gene and its neuropeptide products as an RNAi target from two insect groups; a social insect, the fire ant (Solenopsis invicta) and a non-social insect, the corn earworm (Helicoverpa zea). Both insects are economically important pests. We report negative impacts after PBAN dsRNA treatment to suppress PBAN gene transcription during developmental and adult stages of both species, e.g. increased adult and larval mortality, delayed pupal development and decreased sex pheromone production in the moth. This is an important first step in determining the multiple functions of the PBAN gene in these two insects. This work illustrates the variety of phenotypic effects observed after RNAi silencing of the PBAN gene and suggests the possibility of novel biologically-based insect pest control methods. | Animals, Ants, Female, Gene Expression Regulation, Developmental, Insect Hormones, Insect Proteins, Male, Molecular Sequence Data, Moths, Neuropeptides, Phenotype, RNA Interference, Sex Attractants | null |
22,705,257 | 2013-02-07 | 2024-01-09 | 1878-4372 | Trends in plant science | The photomorphogenic repressors COP1 and DET1: 20 years later. | Lau On Sun, Deng Xing Wang | eng | R01 GM047850 (NIGMS NIH HHS, United States); R37 GM047850 (NIGMS NIH HHS, United States); GM-47850 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Review | Arabidopsis Proteins, CULLIN4 protein, Arabidopsis, Cullin Proteins, DET1 protein, Arabidopsis, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Repressor Proteins, AT2G32950 protein, Arabidopsis, Ubiquitin-Protein Ligases | IM | 22705257, S1360-1385(12)00103-3, 10.1016/j.tplants.2012.05.004 | COP1 and DET1 are among the first repressors of photomorphogenesis to be identified, more than 20 years ago. Discovery of these repressors as conserved regulators of the ubiquitin-proteasome system has established protein degradation as a central theme in light signal transduction. COP1 is a RING E3 ubiquitin ligase that targets key regulators for degradation, and DET1 complexes with COP10 and DDB1, which is proposed to aid in COP1-mediated degradation. Recent studies have strengthened the role of COP1 as a major signaling center. DET1 is also emerging as a chromatin regulator in repressing gene expression. Here, we review current understanding on COP1 and DET1, with a focus on their role as part of two distinct, multimeric CUL4-based E3 ligases. | Arabidopsis, Arabidopsis Proteins, Circadian Rhythm, Cullin Proteins, Flowers, Gene Expression Regulation, Plant, Genes, Plant, Genetic Loci, Intracellular Signaling Peptides and Proteins, Light, Nuclear Proteins, Plant Development, Proteolysis, Repressor Proteins, Signal Transduction, Transcription, Genetic, Ubiquitin-Protein Ligases | null |
22,705,259 | 2013-02-25 | 2012-09-24 | 1579-2129 | Archivos de bronconeumologia | Direct health care costs of occupational asthma in Spain: an estimation from 2008. | García Gómez Montserrat, Urbanos Garrido Rosa, Castañeda López Rosario, López Menduiña Patricia | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705259, S0300-2896(12)00186-X, 10.1016/j.arbres.2012.04.014 | Occupational asthma (OA) is the most common work-related disease in industrialized countries. In 2008, only 556 cases of OA had been diagnosed in Spain, which is quite far from even the most conservative estimates. In this context, the aim of this paper is to estimate the number of asthma cases attributable to the work setting in Spain in 2008 as well as the related health care costs for the same year. | Adult, Asthma, Costs and Cost Analysis, Databases, Factual, Female, Health Care Costs, Health Expenditures, Hospital Costs, Hospital Records, Hospitalization, Humans, Length of Stay, Male, Middle Aged, Occupational Diseases, Patient Discharge, Prevalence, Risk, Spain, Young Adult | null |
22,705,258 | 2013-02-25 | 2018-12-01 | 1579-2129 | Archivos de bronconeumologia | Non-invasive ventilation in an elderly population admitted to a respiratory monitoring unit: causes, complications and one-year evolution. | Segrelles Calvo Gonzalo, Zamora García Enrique, Girón Moreno Rosa, Vázquez Espinosa Emma, Gómez Punter Rosa Mar, Fernandes Vasconcelos Gilda, Valenzuela Claudia, Ancochea Bermúdez Julio | eng | null | Clinical Trial, Journal Article | Vasoconstrictor Agents, Oxygen | IM | 22705258, S0300-2896(12)00187-1, 10.1016/j.arbres.2012.05.001 | To determine the usefulness of non-invasive ventilation (NIV) in elderly patients (≥75) admitted to a respiratory monitoring unit (RMU) during hospitalization and 1 year later in comparison with the results from the younger age group (<75). | Acidosis, Respiratory, Activities of Daily Living, Age Factors, Aged, Aged, 80 and over, Combined Modality Therapy, Disease Management, Female, Follow-Up Studies, Heart Failure, Hospital Mortality, Humans, Kidney Failure, Chronic, Male, Noninvasive Ventilation, Oxygen, Oxygen Inhalation Therapy, Patient Readmission, Prospective Studies, Pulmonary Disease, Chronic Obstructive, Respiratory Care Units, Spain, Tertiary Care Centers, Vasoconstrictor Agents | null |
22,705,260 | 2013-05-06 | 2012-11-19 | 1579-2129 | Archivos de bronconeumologia | Spontaneous pneumomediastinum: is it a sign of severity, or does it depend on the underlying respiratory process? | Rombolá Carlos A, Plenc Mariela S, Atance Pablo León | eng | null | Letter | null | IM | 22705260, S0300-2896(12)00191-3, 10.1016/j.arbres.2012.04.018 | null | Humans, Mediastinal Emphysema, Respiratory Tract Diseases, Severity of Illness Index | null |
22,705,262 | 2012-10-24 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | Synergistic action of Galleria mellonella anionic peptide 2 and lysozyme against Gram-negative bacteria. | Zdybicka-Barabas Agnieszka, Mak Pawel, Klys Anna, Skrzypiec Krzysztof, Mendyk Ewaryst, Fiołka Marta J, Cytryńska Małgorzata | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anions, Peptides, Muramidase | IM | 22705262, S0005-2736(12)00196-4, 10.1016/j.bbamem.2012.06.008 | Lysozyme and antimicrobial peptides are key factors of the humoral immune response in insects. In the present work lysozyme and anionic defense peptide (GMAP2) were isolated from the hemolymph of the greater wax moth Galleria mellonella and their antibacterial activity was investigated. Adsorption of G. mellonella lysozyme on the cell surface of Gram-positive and Gram-negative bacteria was demonstrated using immunoblotting with anti-G. mellonella lysozyme antibodies. Lysozyme effectively inhibited the growth of selected Gram-positive bacteria, which was accompanied by serious alterations of the cell surface, as revealed by atomic force microscopy (AFM) imaging. G. mellonella lysozyme used in concentrations found in the hemolymph of naive and immunized larvae, perforated also the Escherichia coli cell membrane and the level of such perforation was considerably increased by GMAP2. GMAP2 used alone did not perforate E. coli cells nor influence lysozyme muramidase activity. However, the peptide induced a decrease in the turgor pressure of the bacterial cell. Moreover, in the samples of bacteria treated with a mixture of lysozyme and GMAP2 the sodium chloride crystals were found, suggesting disturbance of ion transport across the membrane leading to cell disruption. These results clearly indicated the synergistic action of G. mellonella lysozyme and anionic peptide 2 against Gram-negative bacteria. The reported results suggested that, thanks to immune factors constitutively present in hemolymph, G. mellonella larvae are to some extent protected against infection caused by Gram-negative bacteria. | Animals, Anions, Drug Synergism, Gram-Negative Bacteria, Gram-Positive Bacteria, Microbial Sensitivity Tests, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Moths, Muramidase, Peptides, Spectrometry, X-Ray Emission | null |
22,705,261 | 2013-01-14 | 2017-11-16 | 1579-2129 | Archivos de bronconeumologia | The increased expression of CD11c and CD103 molecules in the neutrophils of the peripheral blood treated with a formula of bacterial ribosomes and proteoglycans of Klebsiella pneumoniae. | Villa-Ambriz Jessica, Rodríguez-Orozco Alain R, Béjar-Lozano Carlos, Cortés-Rojo Christian | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Adjuvants, Immunologic, Antigens, Bacterial, Antigens, CD, CD11c Antigen, Integrin alpha Chains, Proteoglycans, Ribomunyl, alpha E integrins | IM | 22705261, S0300-2896(12)00195-0, 10.1016/j.arbres.2012.04.022 | To evaluate the effect of a preparation with bacterial ribosomes and proteoglycans from Klebsiella pneumoniae «R» on the in vitro expression of CD11c and CD103 molecules in neutrophils from peripheral blood. | Adjuvants, Immunologic, Antigens, Bacterial, Antigens, CD, Blood Cells, CD11c Antigen, Cell Adhesion, Cells, Cultured, Dose-Response Relationship, Drug, Dose-Response Relationship, Immunologic, Gene Expression Regulation, Humans, Integrin alpha Chains, Klebsiella pneumoniae, Neutrophils, Proteoglycans, Ribosomes | null |
22,705,263 | 2012-10-24 | 2017-12-13 | 0006-3002 | Biochimica et biophysica acta | Membrane transport metabolons. | Moraes Trevor F, Reithmeier Reinhart A F | eng | MOP102493 (Canadian Institutes of Health Research, Canada); MOP115182 (Canadian Institutes of Health Research, Canada) | Journal Article, Research Support, Non-U.S. Gov't, Review | ATP-Binding Cassette Transporters, Amino Acids, Membrane Transport Proteins, Glucose | IM | 22705263, S0005-2736(12)00195-2, 10.1016/j.bbamem.2012.06.007 | In this review evidence from a wide variety of biological systems is presented for the genetic, functional, and likely physical association of membrane transporters and the enzymes that metabolize the transported substrates. This evidence supports the hypothesis that the dynamic association of transporters and enzymes creates functional membrane transport metabolons that channel substrates typically obtained from the extracellular compartment directly into their cellular metabolism. The immediate modification of substrates on the inner surface of the membrane prevents back-flux through facilitated transporters, increasing the efficiency of transport. In some cases products of the enzymes are themselves substrates for the transporters that efflux the products in an exchange or antiport mechanism. Regulation of the binding of enzymes to transporters and their mutual activities may play a role in modulating flux through transporters and entry of substrates into metabolic pathways. Examples showing the physical association of transporters and enzymes are provided, but available structural data is sparse. Genetic and functional linkages between membrane transporters and enzymes were revealed by an analysis of Escherichia coli operons encoding polycistronic mRNAs and provide a list of predicted interactions ripe for further structural studies. This article supports the view that membrane transport metabolons are important throughout Nature in organisms ranging from bacteria to humans. | ATP-Binding Cassette Transporters, Amino Acids, Bacteria, Glucose, Glycolysis, Ion Transport, Membrane Transport Proteins, Mitochondria, Models, Molecular, Operon, Yeasts | null |
22,705,265 | 2013-06-20 | 2018-12-02 | 1973-8102 | Cortex; a journal devoted to the study of the nervous system and behavior | Playing the flashbulb memory game: a comment on Cubelli and Della Sala. | Curci Antonietta, Conway Martin A | eng | null | Journal Article, Comment | null | IM | 22705265, S0010-9452(12)00165-7, 10.1016/j.cortex.2012.05.004 | null | Eidetic Imagery, Humans, Life Change Events, Memory, Mental Recall, Violence | null |
22,705,264 | 2013-01-22 | 2018-12-01 | 1532-2823 | Prostaglandins, leukotrienes, and essential fatty acids | Clinical and biochemical validation of two endophenotypes of schizophrenia defined by levels of polyunsaturated fatty acids in red blood cells. | Bentsen H, Solberg D K, Refsum H, Bøhmer T | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antipsychotic Agents, Biomarkers, Dibenzothiazepines, Fatty Acids, Unsaturated, Benzodiazepines, Quetiapine Fumarate, Clozapine, Olanzapine | IM | 22705264, S0952-3278(12)00072-5, 10.1016/j.plefa.2012.05.005 | Polyunsaturated fatty acids (PUFAs) are bimodally distributed in acute schizophrenia, suggesting two endophenotypes. We intended to characterize these endophenotypes clinically. Our a priori hypothesis was that low PUFA patients have more negative symptoms. | Adult, Antipsychotic Agents, Benzodiazepines, Biomarkers, Clozapine, Dibenzothiazepines, Endophenotypes, Erythrocytes, Fatty Acids, Unsaturated, Female, Humans, Hypertriglyceridemia, Linear Models, Logistic Models, Male, Obesity, Olanzapine, Quetiapine Fumarate, Schizophrenia, Schizophrenic Psychology | null |
22,705,270 | 2013-03-07 | 2022-04-09 | 1532-2130 | European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society | Developmental coordination disorder: a review and update. | Zwicker Jill G, Missiuna Cheryl, Harris Susan R, Boyd Lara A | eng | null | Case Reports, Journal Article, Research Support, Non-U.S. Gov't, Review | null | IM | 22705270, S1090-3798(12)00124-9, 10.1016/j.ejpn.2012.05.005 | Present in approximately 5-6% of school-aged children, developmental coordination disorder (DCD) is a neuromotor disability in which a child's motor coordination difficulties significantly interfere with activities of daily living or academic achievement. These children typically have difficulty with fine and/or gross motor skills, with motor performance that is usually slower, less accurate, and more variable than that of their peers. In this paper, we review the history of various definitions leading up to the current definition of DCD, prevalence estimates for the disorder, etiology, common co-morbidities, the impact of DCD on the child's life, and prognosis. As well, we briefly describe current interventions for children with the disorder and results of recent neuroimaging studies of the brains of children with DCD, including research by the authors of this paper. | Adolescent, Child, Child, Preschool, Diagnostic and Statistical Manual of Mental Disorders, Disability Evaluation, Humans, Magnetic Resonance Imaging, Male, Motor Skills Disorders, Neuroimaging, Prevalence, Prognosis | null |
22,705,269 | 2013-06-04 | 2015-11-19 | 1532-2122 | European journal of oncology nursing : the official journal of European Oncology Nursing Society | Arm morbidity and sick leave among working women shortly after breast cancer surgery. | Wennman-Larsen Agneta, Olsson Mariann, Alexanderson Kristina, Nilsson Marie I, Petersson Lena-Marie | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705269, S1462-3889(12)00051-8, 10.1016/j.ejon.2012.05.004 | There is limited knowledge about the impact of arm morbidity on sick leave in the immediate period after breast cancer surgery. | Adult, Arm, Breast Neoplasms, Causality, Comorbidity, Dystonia, Female, Humans, Lymphedema, Middle Aged, Postoperative Complications, Sick Leave, Surveys and Questionnaires, Sweden, Women, Working, Young Adult | null |
22,705,268 | 2012-11-01 | 2018-12-01 | 1769-7123 | Research in microbiology | 10th Francophone Yeast Meeting 'Levures, Modèles & Outils'. | Martin-Yken Hélène, Ribaud Virginie, Poli Jérôme, Hoareau-Aveilla Coralie, Spichal Maya, Beaufort Sandra, Tilloy Valentin, Delerue Thomas, Capp Jean-Pascal, Parrou Jean-Luc | eng | null | Congress | DNA, Fungal | IM | 22705268, S0923-2508(12)00076-9, 10.1016/j.resmic.2012.05.007 | null | DNA, Fungal, Fermentation, Food Microbiology, Gene Expression Regulation, Fungal, Industrial Microbiology, Organelles, Yeasts | null |
22,705,267 | 2013-01-14 | 2022-03-21 | 1879-0542 | Immunology letters | Microvesicles derived from mesenchymal stem cells: potent organelles for induction of tolerogenic signaling. | Mokarizadeh Aram, Delirezh Nowruz, Morshedi Ahmad, Mosayebi Ghasem, Farshid Amir-Abbas, Mardani Karim | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cytokines | IM | 22705267, S0165-2478(12)00158-7, 10.1016/j.imlet.2012.06.001 | Generation and maintenance of immunological tolerance is a pivotal aim in the field of autoimmunity. Regulatory molecules of Programmed Death Ligand-1 (PD-L1), galectin-1 and TGF-β are described as key mediators of peripheral tolerance that actively suppress auto-reactive cells and inhibit their mediated tissue damages. Accordingly, biological intervention in host immune system for induction of peripheral tolerance is pivot to many of the recent studies. Mesenchymal stem cell-derived microvesicles (MVs) are viewed as potential mediators to shed peripheral tolerance toward auto-reactive cells via bearing of tolerogenic molecules. Here, MVs were isolated from mesenchymal stem cell (MSC) cultures' conditioned medium. They were explored for the expression of PD-L1, galectin-1 and membrane bound TGF-β through flow cytometry. The immunoregulatory effects of MVs on splenic mononuclear cells (MNCs) derived from experimental autoimmune encephalomyelitis (EAE) affected mice were investigated using MTT assay, ELISA and flow cytometry. MVs derived from MSCs expressed PD-L1, galecin-1 and membrane-bound TGF-β. MVs exhibited the potential to inhibit auto-reactive lymphocyte proliferation and also the potency to promote them to secret anti-inflammatory cytokines of IL-10 and TGF-β. Interestingly, inducing inflammatory setting on MSCs, revealed the enhancing regulatory effects of MVs via increased expression of some regulatory molecules, specifically PD-L1 and TGF-β. Induction of tolerogenic signaling, promotion of CD4+ CD25+ Foxp3+ regulatory T cells generation and apoptotic activity towards activated T cells are shown to be possible mechanisms involved in MV-mediated regulation. Recent study suggests MSC-derived MVs as potent organelles for induction of peripheral tolerance and modulation of immune responses. | Animals, Apoptosis, Cytokines, Cytoplasmic Vesicles, Encephalomyelitis, Autoimmune, Experimental, Female, Immune Tolerance, Lymphocyte Activation, Mesenchymal Stem Cells, Mice, Organelles, Signal Transduction, T-Lymphocytes, T-Lymphocytes, Regulatory | null |
22,705,266 | 2013-08-12 | 2023-11-06 | 1973-8102 | Cortex; a journal devoted to the study of the nervous system and behavior | Hypnosis in the right hemisphere. | Kihlstrom John F, Glisky Martha L, McGovern Susan, Rapcsak Steven Z, Mennemeier Mark S | eng | HD-055677 (NICHD NIH HHS, United States); R03 HD055269 (NICHD NIH HHS, United States); R01 MH035856 (NIMH NIH HHS, United States); R37 MH035856 (NIMH NIH HHS, United States); R21 HD055677 (NICHD NIH HHS, United States); P20 RR020146 (NCRR NIH HHS, United States); RR-20146 (NCRR NIH HHS, United States) | Case Reports, Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705266, S0010-9452(12)00164-5, 10.1016/j.cortex.2012.04.018, PMC3502707, NIHMS378611, 10768440, 1202204, 19428287, 6630560, 7744557, 14768966, 1541575, 8783218, 4417693, 681034, 8468107, 5682831, 4448866, 7178387, 10023510, 8292325, 9003980, 7897034, 3699937, 4032328, 8166843, 13988658, 3627927, 7805389, 9950718, 8475220, 737261, 7413391, 7082709, 422805, 2592955, 21707128, 22748566, 9077052, 3596845, 5164381, 5808986, 5485330, 6542915, 7229904, 2295536, 539566, 1723332, 12934845 | Speculations about the neural substrates of hypnosis have often focused on the right hemisphere (RH), implying that RH damage should impair hypnotic responsiveness more than left-hemisphere (LH) damage. The present study examined the performance of a patient who suffered a stroke destroying most of his LH, on slightly modified versions of two hypnotizability scales. This patient was at least modestly hypnotizable, as indicated in particular by the arm rigidity and age regression items, suggesting that hypnosis can be mediated by the RH alone - provided that the language capacities normally found in the LH remain available. A further study of 16 patients with unilateral strokes of the LH or RH found no substantial differences in hypnotizability between the two groups. Future neuropsychological studies of hypnosis might explore the dorsal/ventral or anterior/posterior dichotomies, with special emphasis on the role of prefrontal cortex. | Brain, Cognition, Female, Functional Laterality, Hemiplegia, Humans, Hypnosis, Language, Male, Middle Aged, Neuropsychological Tests, Prefrontal Cortex, Psychometrics, Radiography, Stroke, Stroke Rehabilitation, Suggestion | null |
22,705,271 | 2013-03-12 | 2012-08-27 | 1532-2130 | European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society | Dravet syndrome: the main issues. | Guerrini Renzo | eng | null | Journal Article, Review | NAV1.1 Voltage-Gated Sodium Channel, SCN1A protein, human | IM | 22705271, S1090-3798(12)00096-7, 10.1016/j.ejpn.2012.04.006 | Dravet syndrome (DS) is a severe form of infantile onset epilepsy characterized by multiple seizure types, prolonged convulsive seizures and frequent episodes of status epilepticus. Seizures precipitated by fever are a main characteristic. Affected children exhibit normal early development. Cognitive impairment, behavioral disturbances with hyperactivity and sometimes autistic traits occur after seizure onset. Seizures persist into adulthood but become less frequent. In about 85% of patients, a mutation of the SCN1A gene is present. DS fully illustrates the concept of epileptic encephalopathy. However, it is difficult to determine the causative role of the underlying sodium channel dysfunction and that of the consequent seizures in influencing cognitive outcome. An overwhelmingly high number of SCN1A mutations have been associated with DS. Intragenic or whole gene deletions, duplications and amplifications are additional rare molecular mechanisms. Most mutations are de novo, but familial mutations also occur. Somatic mosaic mutations should be considered when estimating the recurrence. MRI imaging is usually normal, and no neuropathologic signature of the condition seems to exist. In heterozygous Scn1a+/- mice, GABAergic interneurons exhibit substantially reduced sodium current density with reduced ability for sustained action potential firing. GABAergic output is reduced and excitability of downstream synaptic targets increased. Stiripentol was effective in combination with valproate and clobazam in two pivotal phase III trials. Phenytoin, carbamazepine, and lamotrigine can worsen seizures and should be avoided. Prospective studies will clarify to what extent earlier diagnosis and efforts at seizure control with the most appropriate drug combinations will reduce clinical deterioration. | Animals, Epilepsies, Myoclonic, Humans, Mutation, NAV1.1 Voltage-Gated Sodium Channel, Seizures | null |
22,705,272 | 2013-01-16 | 2015-11-19 | 1095-8304 | Appetite | Weight regulation practices of young adults. Predictors of restrictive eating. | Quick Virginia M, Byrd-Bredbenner Carol | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705272, S0195-6663(12)00205-X, 10.1016/j.appet.2012.06.004 | Young adults frequently use restrictive eating (i.e., going for long periods [≥ 8h] without eating to influence their shape or weight) to control their weight. The purpose of this study was to determine the prevalence of restrictive eating in young adults, compare eating behaviors of restrictive and non-restrictive eaters, and predict restrictive eaters. A diverse (56% white, 63% female) sample of young adults (n=2449) completed an online survey that included eating behavior scales (Restraint, Eating, Shape, and Weight Concerns, and Inappropriate Compensatory Behaviors from the Eating Disorder Examination-Questionnaire, Emotional and Disinhibited Eating from the Three-Factor Eating Questionnaire, and Night Eating from the Night Eating Questionnaire) and demographics. A quarter of women and 20% of men were classified as restrictive eaters. Independent t-tests revealed restrictive eaters had significantly (p<0.001) higher BMIs than non-restrictive eaters. Restrictive eaters also had significantly higher scores on all eating behavior scales than non-restrictive eaters even after controlling for potential confounding factors (BMI, race). Stepwise binary logistic regression revealed that increased eating, shape, and weight concerns, higher BMI, endorsement of inappropriate compensatory behaviors and night eating, being female, and white increased the odds of participants being restrictive eaters. This study can help healthcare professionals become more aware of weight control practices of young adults and create appropriate interventions. | Adolescent, Adult, Body Weight, Cross-Sectional Studies, Eating, Feeding Behavior, Feeding and Eating Disorders, Female, Forecasting, Humans, Logistic Models, Male, Prevalence, Surveys and Questionnaires, Young Adult | null |
22,705,273 | 2013-09-06 | 2023-12-13 | 1873-7064 | Neuropharmacology | Cation-chloride cotransporters NKCC1 and KCC2 as potential targets for novel antiepileptic and antiepileptogenic treatments. | Löscher Wolfgang, Puskarjov Martin, Kaila Kai | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Anticonvulsants, Diuretics, Receptors, GABA-A, SLC12A2 protein, human, Sodium-Potassium-Chloride Symporters, Solute Carrier Family 12, Member 2, Symporters, Bumetanide, gamma-Aminobutyric Acid | IM | 22705273, S0028-3908(12)00253-5, 10.1016/j.neuropharm.2012.05.045 | In cortical and hippocampal neurons, cation-chloride cotransporters (CCCs) control the reversal potential (EGABA) of GABAA receptor-mediated current and voltage responses and, consequently, they modulate the efficacy of GABAergic inhibition. Two members of the CCC family, KCC2 (the major neuron-specific K-Cl cotransporter; KCC isoform 2) and NKCC1 (the Na-K-2Cl cotransporter isoform 1 which is expressed in both neurons and glial cells) have attracted much interest in studies on GABAergic signaling under both normal and pathophysiological conditions, such as epilepsy. There is tentative evidence that loop diuretic compounds such as furosemide and bumetanide may have clinically relevant antiepileptic actions, especially when administered in combination with conventional GABA-mimetic drugs such as phenobarbital. Furosemide is a non-selective inhibitor of CCCs while at low concentrations bumetanide is selective for NKCCs. Search for novel antiepileptic drugs (AEDs) is highly motivated especially for the treatment of neonatal seizures which are often resistant to, or even aggravated by conventional AEDs. This review shows that the antiepileptic effects of loop diuretics described in the pertinent literature are based on widely heterogeneous mechanisms ranging from actions on both neuronal NKCC1 and KCC2 to modulation of the brain extracellular volume fraction. A promising strategy for the development of novel CCC-blocking AEDs is based on prodrugs that are activated following their passage across the blood-brain barrier. This article is part of the Special Issue entitled 'New Targets and Approaches to the Treatment of Epilepsy'. | Adult, Animals, Anticonvulsants, Brain, Bumetanide, Diuretics, Epilepsy, Humans, Infant, Newborn, Receptors, GABA-A, Sodium-Potassium-Chloride Symporters, Solute Carrier Family 12, Member 2, Symporters, gamma-Aminobutyric Acid, K Cl- Cotransporters | null |
22,705,274 | 2012-12-21 | 2012-08-03 | 1873-6823 | Alcohol (Fayetteville, N.Y.) | Take me away: the relationship between escape drinking and attentional bias for alcohol-related cues. | Forestell Catherine A, Dickter Cheryl L, Young Chelsie M | eng | null | Journal Article | null | IM | 22705274, S0741-8329(12)00083-3, 10.1016/j.alcohol.2012.05.001 | Previous research has indicated that implicit attentional bias to alcohol-related cues may serve as a cognitive measure of susceptibility to alcohol dependence. The primary goal of the current study was to examine whether college students who drink to escape dysphoric emotions or moods (i.e., escape drinkers) have stronger attentional biases for alcohol-related cues than non-escape drinkers. Additionally, because previous research has shown that presentation time and content of smoking-related stimuli moderates differences between smokers' and nonsmokers' reaction times, this study sought to determine whether these effects generalized to alcohol-related stimuli. Participants who were identified as either escape (n = 74) or non-escape drinkers (n = 48) completed a dot-probe task in which alcohol-related pictures that contained humans interacting with the alcohol-related cues (active) or alcohol-related cues alone (inactive) were presented along with matched control pictures. These stimuli were presented for either 500 ms or 2000 ms to determine whether attentional biases occur as a function of initial or maintained attention to the alcohol-related cues. Escape drinkers displayed a significantly stronger attentional bias for alcohol-related inactive cues at longer presentation times (i.e., 2000 ms) compared to non-escape drinkers. This bias was independent of alcohol dependence and family history of alcoholism. These results suggest that in addition to dependence and family history, escape drinking is an important factor to consider when examining attentional biases to alcohol-related cues. | Alcohol Drinking, Alcoholism, Attention, Binge Drinking, Cues, Female, Humans, Male, Reaction Time, Smoking, Young Adult | null |
22,705,275 | 2013-03-12 | 2012-09-17 | 1773-0597 | Journal francais d'ophtalmologie | Central serous retinal detachment following glaucoma filtration surgery. | Aydin A, Cakir A, Unal M H, Ersanli D | eng | null | Case Reports, Journal Article | null | IM | 22705275, S0181-5512(12)00139-8, 10.1016/j.jfo.2011.10.010 | We report the occurrence of central serous retinal detachment in association with hypotony in a patient that had undergone glaucoma filtration surgery. A 35-year-old man, who had a recent closed-globe injury, underwent trabeculectomy with mitomycin C for intractable traumatic glaucoma. On the second day after surgery, the eye became hypotonous and the patient's visual acuity decreased abruptly to counting fingers. Fundoscopy and optical coherence tomography revealed a central serous retinal detachment. Fundus florescein angiography showed choroidal leakage from traumatic perifoveal retinal pigment epithelial defects. Although the hypotony resolved within the first postoperative week, it took 2 months for the central serous retinal detachment to clear. To our knowledge, this is the first reported case of isolated central serous retinal detachment following glaucoma filtration surgery. | Adult, Filtering Surgery, Fluorescein Angiography, Glaucoma, Humans, Male, Retinal Detachment, Tomography, Optical Coherence | null |
22,705,276 | 2012-12-10 | 2012-07-24 | 1695-9531 | Anales de pediatria (Barcelona, Spain : 2003) | [Obesity prevention from physical activity: from theoretical discourse to practice]. | Moreno L A, Gracia-Marco L | spa | null | English Abstract, Journal Article, Practice Guideline | null | IM | 22705276, S1695-4033(12)00248-2, 10.1016/j.anpedi.2012.04.011 | Childhood obesity has increased considerably in most countries in recent years. Obese children already have many co-morbidities since infancy, which can have serious consequences in adulthood. From a health standpoint, the most appropriate to address this problem is primary prevention. This article aims to summarize the relevant aspects from the point of view of prevention of childhood obesity, and in particular to those related to physical activity. To this end, health and education professionals have a role. In all cases, it is necessary to perform the evaluation of programs to see if they are really effective. Developing new programs should be based on previous experiences that had positive results. As most interventions to date have not been very effective, much more research is needed in this area in the future. | Adolescent, Child, Child, Preschool, Health Promotion, Humans, Infant, Motor Activity, Obesity | null |
22,705,277 | 2014-01-06 | 2016-11-25 | 1695-9531 | Anales de pediatria (Barcelona, Spain : 2003) | [Diet and nutrition practices and the socioeconomic situation in homes with premature infants in Guanajuato (Mexico)]. | Monroy Torres R, López López M, Naves Sánchez J | spa | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705277, S1695-4033(12)00254-8, 10.1016/j.anpedi.2012.05.001 | To determine diet and nutrition practices and the economic and social situation in homes with premature infants. | Adolescent, Adult, Cross-Sectional Studies, Diet, Diet Surveys, Feeding Behavior, Humans, Infant, Newborn, Infant, Premature, Mexico, Middle Aged, Socioeconomic Factors, Young Adult | null |
22,705,280 | 2013-07-18 | 2022-03-17 | 1096-3650 | Seminars in cancer biology | Current and future systemic treatments for renal cell carcinoma. | Fisher Rosalie, Gore Martin, Larkin James | eng | null | Journal Article, Review | null | IM | 22705280, S1044-579X(12)00096-X, 10.1016/j.semcancer.2012.06.004 | Systemic treatment of renal cell carcinoma has changed dramatically since 2007, with the development and approval of six new agents, which target complex molecular pathways regulating tumour angiogenesis and cell proliferation and survival. These treatments have significantly improved survival times in metastatic renal cell carcinoma, but remain palliative. A number of newer agents are in clinical development, which offer theoretical advantages over existing treatments, and research methodologies are adapting with the aim of defining an individualised approach to therapy which exploits the underlying tumour biology. This review will provide an overview of current and emerging systemic treatments and how they might be integrated with surgical therapy, with a particular focus on advanced, clear cell metastatic renal cell carcinoma. | Carcinoma, Renal Cell, Humans, Kidney Neoplasms | null |
22,705,281 | 2013-06-26 | 2012-09-03 | 1532-3072 | Tissue & cell | The oviduct is a brood chamber for facultative egg retention in the parthenogenetic oribatid mite Archegozetes longisetosus AOKI (Acari, Oribatida). | Bergmann P, Heethoff M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705281, S0040-8166(12)00060-2, 10.1016/j.tice.2012.05.002 | Archegozetes longisetosus is a parthenogenetic oribatid mite and a chelicerate model organism. We examined the localisation of processes between vitellogenesis and embryogenesis as well as the anatomy and histology of involved structures by means of light- and electron microscopy. The proximal oviduct is differentiated into an oviductal bulb, exhibiting a strong secretory epithelium. Here, solidification of the egg shell instantaneously occurs upon passing of the egg from the perivitelline space into the oviductal lumen. This is interpreted as an internalised oviposition with the generation boundary being effectively located at the ovary-oviduct transition, rendering the oviducts into functional brood chambers. The parity mode combines elements of oviparity and ovolarviparity with facultative egg retention. | Acari, Animals, Female, Microscopy, Electron, Oviducts, Parthenogenesis, Vitellogenesis | null |
22,705,279 | 2013-07-18 | 2024-06-10 | 1096-3650 | Seminars in cancer biology | The metabolic basis of kidney cancer. | Linehan W Marston, Ricketts Christopher J | eng | Z01 BC011089 (Intramural NIH HHS, United States); Z01 BC011089-01 (Intramural NIH HHS, United States); Z01 BC011023 (Intramural NIH HHS, United States); Z01 BC011038 (Intramural NIH HHS, United States); Z01 BC011023-01 (Intramural NIH HHS, United States); Z01 BC011043-01 (Intramural NIH HHS, United States); Z01 BC011028-01 (Intramural NIH HHS, United States); Z01 BC011038-01 (Intramural NIH HHS, United States); Z01 BC011043 (Intramural NIH HHS, United States); Z01 BC011028 (Intramural NIH HHS, United States) | Journal Article, Research Support, N.I.H., Intramural, Review | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, FLCN protein, human, Proto-Oncogene Proteins, Tumor Suppressor Proteins, Von Hippel-Lindau Tumor Suppressor Protein | IM | 22705279, S1044-579X(12)00094-6, 10.1016/j.semcancer.2012.06.002, PMC3563773, NIHMS392241, 21907923, 18757421, 21218461, 14634372, 17895761, 22012259, 18403135, 21209915, 21074195, 9140397, 1985294, 10353251, 17509289, 17028174, 17296901, 19995375, 10657297, 9829911, 12204536, 20100962, 12772087, 9854597, 20448661, 12814730, 17322109, 19274086, 18063061, 8493574, 18676741, 11062460, 11248088, 8995477, 7660122, 9187702, 8872474, 14685938, 15371818, 18182616, 596896, 10878807, 15956655, 15201738, 17920899, 9393982, 18781191, 8863669, 10213691, 10433944, 11927500, 11176466, 20421778, 17215529, 18234728, 12957289, 12890841, 7587394, 7853572, 12719541, 2276737, 11395386, 15261137, 10647647, 19184535, 16098467, 19850877, 22022277, 7500446, 19963135, 9122164, 11292861, 18653228, 11865300, 17538086, 15328326, 20073616, 7660130, 20679884, 12612654, 12459621, 8308957, 17005952, 20154303, 17215530, 10210376, 7966777, 18728283, 18184959, 19270154, 12938083, 12459622, 15035284, 13298683 | Kidney cancer is not a single disease; it is made up of a number of different types of cancer that occur in the kidney. Each of these different types of kidney cancer can have a different histology, have a different clinical course, can respond differently to therapy and is caused by a different gene. Kidney cancer is essentially a metabolic disease; each of the known genes for kidney cancer, VHL, MET, FLCN, TSC1, TSC2, TFE3, TFEB, MITF, fumarate hydratase (FH), succinate dehydrogenase B (SDHB), succinate dehydrogenase D (SDHD), and PTEN genes is involved in the cells ability to sense oxygen, iron, nutrients or energy. Understanding the metabolic basis of kidney cancer will hopefully provide the foundation for the development of effective forms of therapy for this disease. | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Carcinoma, Renal Cell, Citric Acid Cycle, Humans, Kidney Neoplasms, Mutation, Proto-Oncogene Proteins, Tuberous Sclerosis, Tumor Suppressor Proteins, Von Hippel-Lindau Tumor Suppressor Protein | null |
22,705,283 | 2013-11-27 | 2022-12-07 | 1578-2190 | Actas dermo-sifiliograficas | Retroauricular revolving door island flap. | Russo de la Torre F | eng | null | Journal Article, Video-Audio Media | null | IM | 22705283, S0001-7310(12)00156-1, 10.1016/j.ad.2012.04.001 | null | Ear, External, Humans, Plastic Surgery Procedures, Surgical Flaps | null |
22,705,278 | 2013-07-18 | 2022-04-08 | 1096-3650 | Seminars in cancer biology | The VHL/HIF axis in clear cell renal carcinoma. | Shen Chuan, Kaelin William G | eng | P50 CA101942 (NCI NIH HHS, United States); R01 CA068490 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | Hypoxia-Inducible Factor 1, Platelet-Derived Growth Factor, Vascular Endothelial Growth Factors, Von Hippel-Lindau Tumor Suppressor Protein | IM | 22705278, S1044-579X(12)00093-4, 10.1016/j.semcancer.2012.06.001, PMC3663044, NIHMS392693, 12119283, 2720695, 20660729, 10454537, 16510872, 12086860, 19252501, 12727920, 15900031, 17708671, 10353251, 19706526, 11498541, 9816062, 16585196, 19955413, 18728026, 20100962, 14691554, 18156031, 17872411, 17876014, 11641274, 15585612, 19255431, 12538644, 19471969, 20863703, 20951770, 19061835, 19805192, 12124175, 3275797, 15210739, 11159196, 18498744, 21124777, 15302861, 19224847, 11331613, 9443397, 22169972, 18490920, 17322920, 16126907, 15611513, 18339845, 17526729, 17201148, 16858007, 17613433, 16158924, 11292862, 18984585, 16729911, 2354442, 17404621, 11595184, 20008644, 17063461, 20431476, 14985465, 21131975, 12097293, 12080085, 19528226, 14701857, 17244529, 19920202, 17804822, 21881266, 12036906, 17215529, 17045088, 9146668, 12351678, 18281490, 18093943, 21830968, 16778202, 15556999, 12726861, 11598268, 19858293, 15798202, 18272024, 11490304, 9521897, 10785600, 12743597, 18945681, 16452206, 21725288, 18039096, 16977322, 12042299, 16585195, 11292861, 21725364, 22037472, 18653228, 15964822, 11504942, 18297059, 19124809, 17538086, 12957288, 9385930, 17637739, 12086861, 16380835, 21353547, 17463250, 18838541, 19111663, 11331612, 16971129, 9413424, 17215530, 16760477, 16733217, 17670948, 20606035, 18713068, 20679489, 19402058, 9697772, 17220275, 18948595, 10637252, 19470766, 20643778, 20054297, 20032376, 12209156, 11734856, 20439632, 9916127, 20354189, 19285840, 15958567, 21248752, 18538129 | Inactivation of the VHL tumor suppressor protein (pVHL) is a common event in clear cell renal carcinoma, which is the most common form of kidney cancer. pVHL performs many functions, including serving as the substrate recognition module of an ubiquitin ligase complex that targets the alpha subunits of the heterodimeric HIF transcription factor for proteasomal degradation. Deregulation of HIF2α appears to be a driving force in pVHL-defective clear cell renal carcinomas. In contrast, genetic and functional studies suggest that HIF1α serves as a tumor suppressor and is a likely target of the 14q deletions that are characteristic of this tumor type. Drugs that inhibit HIF2α, or its downstream targets such as VEGF, are in various stages of clinical testing. Indeed, clear cell renal carcinomas are exquisitely sensitive to VEGF deprivation and four VEGF inhibitors have now been approved for the treatment of this disease. | Carcinoma, Renal Cell, Gene Expression Regulation, Neoplastic, Humans, Hypoxia-Inducible Factor 1, Platelet-Derived Growth Factor, Vascular Endothelial Growth Factors, Von Hippel-Lindau Tumor Suppressor Protein | null |
22,705,284 | 2014-01-21 | 2022-04-09 | 1578-2190 | Actas dermo-sifiliograficas | Deep vein thrombosis in a patient with lepromatous leprosy receiving thalidomide to treat leprosy reaction. | Hebe Petiti-Martin G, Villar-Buill M, de la Hera I, Fuertes L, Burgués-Calderón M, Rivera-Díaz R, Vanaclocha F | eng | null | Case Reports, Journal Article | Leprostatic Agents, Thalidomide | IM | 22705284, S0001-7310(12)00048-8, 10.1016/j.ad.2011.12.002 | Thalidomide is the treatment of choice for severe or recurrent erythema nodosum leprosum. Its use has been associated with deep vein thrombosis in patients with blood disorders, however, particularly when used in combination with corticosteroids or chemotherapy. We describe a case of deep vein thrombosis in a 43-year-old man with lepromatous leprosy who was being treated with thalidomide and prednisone for a type 2 leprosy reaction (erythema nodosum leprosum); the patient also had transiently positive antiphospholipid antibody results. We stress the importance of considering deep vein thrombosis, a potentially fatal complication, in dermatology patients treated with thalidomide. | Adult, Humans, Leprostatic Agents, Leprosy, Lepromatous, Male, Thalidomide, Venous Thrombosis | null |
22,705,285 | 2013-01-16 | 2024-01-04 | 1089-8638 | Journal of molecular biology | Silent substitutions predictably alter translation elongation rates and protein folding efficiencies. | Spencer Paige S, Siller Efraín, Anderson John F, Barral José M | eng | UL1 TR000071 (NCATS NIH HHS, United States); P30-AG-024832 (NIA NIH HHS, United States); P30 AG024832 (NIA NIH HHS, United States); UL1 RR029876 (NCRR NIH HHS, United States); UL1RR029876 (NCRR NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Codon, Proteins, RNA, Transfer | IM | 22705285, S0022-2836(12)00483-4, 10.1016/j.jmb.2012.06.010, PMC3576719, NIHMS440588, 17185560, 1960727, 16213112, 18984615, 18538657, 19783824, 12761062, 20043920, 9770666, 17897886, 21567957, 14759365, 3916708, 22221219, 6084718, 18552770, 2478714, 19359587, 5969078, 22045228, 24688635, 6396082, 17194224, 19931533, 10809680 | Genetic code redundancy allows most amino acids to be encoded by multiple codons that are non-randomly distributed along coding sequences. An accepted theory explaining the biological significance of such non-uniform codon selection is that codons are translated at different speeds. Thus, varying codon placement along a message may confer variable rates of polypeptide emergence from the ribosome, which may influence the capacity to fold toward the native state. Previous studies report conflicting results regarding whether certain codons correlate with particular structural or folding properties of the encoded protein. This is partly due to different criteria traditionally utilized for predicting translation speeds of codons, including their usage frequencies and the concentration of tRNA species capable of decoding them, which do not always correlate. Here, we developed a metric to predict organism-specific relative translation rates of codons based on the availability of tRNA decoding mechanisms: Watson-Crick, non-Watson-Crick or both types of interactions. We determine translation rates of messages by pulse-chase analyses in living Escherichia coli cells and show that sequence engineering based on these concepts predictably modulates translation rates in a manner that is superior to codon usage frequency, which occur during the elongation phase, and significantly impacts folding of the encoded polypeptide. Finally, we demonstrate that sequence harmonization based on expression host tRNA pools, designed to mimic ribosome movement of the original organism, can significantly increase the folding of the encoded polypeptide. These results illuminate how genetic code degeneracy may function to specify properties beyond amino acid encoding, including folding. | Amino Acid Substitution, Base Sequence, Codon, Escherichia coli, Genetic Code, Molecular Sequence Data, Peptide Chain Elongation, Translational, Protein Biosynthesis, Protein Folding, Proteins, RNA, Transfer, Ribosomes | null |
22,705,282 | 2013-01-14 | 2022-12-07 | 1873-460X | Journal of diabetes and its complications | Clinical implication of plasma and urine YKL-40, as a proinflammatory biomarker, on early stage of nephropathy in type 2 diabetic patients. | Lee Jun Ho, Kim Sang Soo, Kim In Joo, Song Sang Heon, Kim Yong Ki, In Kim Ju, Jeon Yun Kyung, Kim Bo Hyun, Kwak Ihm Soo | eng | null | Journal Article | Adipokines, Biomarkers, Blood Glucose, CHI3L1 protein, human, Chitinase-3-Like Protein 1, Glycated Hemoglobin A, Lectins | IM | 22705282, S1056-8727(12)00111-0, 10.1016/j.jdiacomp.2012.04.012 | Chronic inflammation has emerged as being a key pathophysiology in the early stages of diabetic nephropathy. YKL-40 has been established as an inflammatory marker in chronic inflammation. The aim of this study was to evaluate the association of plasma and urine YKL-40 with albuminuria in the early stage of type 2 diabetic nephropathy. | Adipokines, Adult, Aged, Albuminuria, Biomarkers, Blood Glucose, Case-Control Studies, Chitinase-3-Like Protein 1, Diabetes Mellitus, Type 2, Diabetic Nephropathies, Disease Progression, Female, Glomerular Filtration Rate, Glycated Hemoglobin, Humans, Inflammation, Lectins, Male, Middle Aged, Severity of Illness Index | null |
22,705,287 | 2013-02-25 | 2024-06-10 | 1361-8423 | Medical image analysis | Strategies for improved interpretation of computer-aided detections for CT colonography utilizing distributed human intelligence. | McKenna Matthew T, Wang Shijun, Nguyen Tan B, Burns Joseph E, Petrick Nicholas, Summers Ronald M | eng | ZIA CL040003 (Intramural NIH HHS, United States); ZIA CL040003-09 (Intramural NIH HHS, United States) | Journal Article, Research Support, N.I.H., Intramural, Research Support, U.S. Gov't, P.H.S. | null | IM | 22705287, S1361-8415(12)00053-9, 10.1016/j.media.2012.04.007, PMC3443285, NIHMS387696, 19235388, 18799557, 15082698, 20443468, 20570766, 20663975, 20228384, 9612889, 19789221, 17209116, 20610543, 7643805, 22274839, 15554123, 20686574, 14657426, 12239037, 12949715, 12933484, 16344052, 20953299, 20457419, 18096536, 19544802, 18703711, 18710990, 18682584, 15664225 | Computer-aided detection (CAD) systems have been shown to improve the diagnostic performance of CT colonography (CTC) in the detection of premalignant colorectal polyps. Despite the improvement, the overall system is not optimal. CAD annotations on true lesions are incorrectly dismissed, and false positives are misinterpreted as true polyps. Here, we conduct an observer performance study utilizing distributed human intelligence in the form of anonymous knowledge workers (KWs) to investigate human performance in classifying polyp candidates under different presentation strategies. We evaluated 600 polyp candidates from 50 patients, each case having at least one polyp ≥6 mm, from a large database of CTC studies. Each polyp candidate was labeled independently as a true or false polyp by 20 KWs and an expert radiologist. We asked each labeler to determine whether the candidate was a true polyp after looking at a single 3D-rendered image of the candidate and after watching a video fly-around of the candidate. We found that distributed human intelligence improved significantly when presented with the additional information in the video fly-around. We noted that performance degraded with increasing interpretation time and increasing difficulty, but distributed human intelligence performed better than our CAD classifier for "easy" and "moderate" polyp candidates. Further, we observed numerous parallels between the expert radiologist and the KWs. Both showed similar improvement in classification moving from single-image to video interpretation. Additionally, difficulty estimates obtained from the KWs using an expectation maximization algorithm correlated well with the difficulty rating assigned by the expert radiologist. Our results suggest that distributed human intelligence is a powerful tool that will aid in the development of CAD for CTC. | Aged, Algorithms, Artificial Intelligence, Colonic Polyps, Colonography, Computed Tomographic, Colorectal Neoplasms, Female, Humans, Intestinal Polyps, Male, Middle Aged, Pattern Recognition, Automated, Radiographic Image Enhancement, Radiographic Image Interpretation, Computer-Assisted, Reproducibility of Results, Sensitivity and Specificity | null |
22,705,286 | 2013-01-16 | 2021-10-21 | 1089-8638 | Journal of molecular biology | The brace for a growing scaffold: Mss116 protein promotes RNA folding by stabilizing an early assembly intermediate. | Fedorova Olga, Pyle Anna Marie | eng | R01 GM050313 (NIGMS NIH HHS, United States); GM50313 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Nucleotides, RNA, Catalytic, Saccharomyces cerevisiae Proteins, RNA, MSS116 protein, S cerevisiae, DEAD-box RNA Helicases | IM | 22705286, S0022-2836(12)00435-4, 10.1016/j.jmb.2012.05.037, PMC4419380, NIHMS680259, 21501623, 12440999, 18803382, 19616559, 19952115, 8670849, 19343072, 11574482, 12574513, 21712813, 21045544, 17960235, 9096319, 21821049, 17196976, 15173385, 18237742, 16872630, 17188036, 14636593, 21679717, 16314300, 20138894, 10438622, 9506945, 7574489, 19154736, 11786013, 8117737, 16023137, 18772388, 21760622, 16936318, 20944626, 17143279, 16216083, 17081564, 19088201, 9360610, 10361090, 10208815, 20554854, 10829274, 9843513, 12173929, 18022197, 21045543, 2684776, 18388288, 9582093, 21779027, 18347437, 10581546, 20446804, 15618406, 12431436, 16337753, 9204274, 12691747, 18502978, 9677291, 9521704, 19393667, 17936712, 11497432, 16214167, 9666325 | The ai5γ group II intron requires a protein cofactor to facilitate native folding in the cell. Yeast protein Mss116 greatly accelerates intron folding under near-physiological conditions both in vivo and in vitro. Although the effect of Mss116 on the kinetics of ai5γ ribozyme folding and catalysis has been extensively studied, the precise structural role and interaction sites of Mss116 have been elusive. Using Nucleotide Analog Interference Mapping to study the folding of splicing precursor constructs, we have identified specific intron functional groups that participate in Mss116-facilitated folding and we have determined their role in the folding mechanism. The data indicate that Mss116 stabilizes an early, obligate folding intermediate within intron domain 1, thereby laying the foundation for productive folding to the native state. In addition, the data reveal an important role for the IBS2 exon sequence and for the terminus of domain 6, during the folding of self-splicing group IIB intron constructs. | Base Sequence, Binding Sites, Catalysis, DEAD-box RNA Helicases, Exons, Introns, Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, Nucleotides, RNA, RNA Folding, RNA Splicing, RNA, Catalytic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins | null |
22,705,288 | 2013-03-12 | 2012-09-21 | 1361-8423 | Medical image analysis | Tissue metabolism driven arterial tree generation. | Schneider Matthias, Reichold Johannes, Weber Bruno, Székely Gábor, Hirsch Sven | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705288, S1361-8415(12)00057-6, 10.1016/j.media.2012.04.009 | We present an approach to generate 3-D arterial tree models based on physiological principles while at the same time certain morphological properties are enforced at construction time. The driving force of the construction is a simplified angiogenesis model incorporating case-specific information about the metabolic demand within the considered domain. The vascular tree is constructed iteratively by successively adding new segments in chemotactic response to angiogenic growth factors secreted by ischemic cells. Morphometrically confirmed bifurcation statistics of vascular networks are incorporated to optimize the synthetic vasculature. The proposed method is able to generate artificial, yet physiologically plausible, arterial tree models that match the metabolic demand of the embedding tissue and fulfill the prescribed morphological properties at the same time. The proposed tree generation approach is applied in a simulation setup based on the metabolic configuration and anatomy of the macaque visual cortex. We analyze the generated tree models with respect to morphological and physiological aspects including fluid-dynamic simulations. The comparison of our results with the findings of different studies on the structure of cerebral vasculatures demonstrates the plausibility of our method. | Animals, Cerebral Arteries, Cerebrovascular Circulation, Computer Simulation, Energy Metabolism, Macaca, Models, Anatomic, Models, Cardiovascular, Visual Cortex | null |
22,705,290 | 2013-04-23 | 2018-12-01 | 1090-2414 | Ecotoxicology and environmental safety | Mitochondrial energy metabolism dysfunction involved in reproductive toxicity of mice caused by endosulfan and protective effects of vitamin E. | Wang Na, Qian Hong-Yan, Zhou Xian-Qing, Li Yan-Bo, Sun Zhi-Wei | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antioxidants, Insecticides, Vitamin E, Malondialdehyde, Adenosine Triphosphate, Endosulfan | IM | 22705290, S0147-6513(12)00164-9, 10.1016/j.ecoenv.2012.05.014 | The experiment was designed to study the mechanism of reproductive toxicity caused by endosulfan in mice and protective effects of vitamin E. The experiment was composed of three groups: the control group did not receive any endosulfan and vitamin E; the endosulfan exposed group received 0.8 mg/kg/d endosulfan and 0mg/kg/d vitamin E; and the endosulfan+vitamin E group received 0.8 mg/kg/d endosulfan and 100mg/kg/d vitamin E. The results showed that vitamin E significantly reversed the decline of the concentration and motility rate of sperm, and inhibited the increase of sperm abnormality rate caused by endosulfan. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and lactate dehydrogenase-C4 (LDH-C4) and the level of adenosine triphosphate (ATP) in the endosulfan+vitamin E group were higher while the malondialdehyde (MDA) content was significantly lower than those of the endosulfan exposed group. The results from pathology and electron microscope observed showed vitamin E decreased the cavities formation by desquamating of spermatogenic cells, stopped the ruptures and disappearances of mitochondrial cristaes in spermatogenic cells, and prevented the breakages and partial dissolvings of sperm tails induced by endosulfan. It is likely that endosulfan could directly damage sperm structures by oxidative stress, leading to a decrease in sperm quantity and quality. It also could indirectly cause a decline in reproductive function by damaging the structure of mitochondria, resulting in energy metabolism dysfunction, which could be one of the mechanisms behind the reproductive toxicity induced by endosulfan. It was inferred that vitamin E helps maintain the structural integrities of sperm architecture and prevent mitochondrial dysfunction through inhibiting oxidative stress, and thereby prevent the reproductive dysfunctions caused by endosulfan. | Adenosine Triphosphate, Animals, Antioxidants, Endosulfan, Energy Metabolism, Enzyme Activation, Insecticides, Lipid Peroxidation, Male, Malondialdehyde, Mice, Mitochondria, Oxidative Stress, Random Allocation, Reproduction, Spermatozoa, Testis, Vitamin E | null |
22,705,289 | 2013-02-25 | 2012-09-14 | 1361-8423 | Medical image analysis | A spline-based non-linear diffeomorphism for multimodal prostate registration. | Mitra Jhimli, Kato Zoltan, Martí Robert, Oliver Arnau, Lladó Xavier, Sidibé Désiré, Ghose Soumya, Vilanova Joan C, Comet Josep, Meriaudeau Fabrice | eng | CNK80370 (PHS HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | null | IM | 22705289, S1361-8415(12)00052-7, 10.1016/j.media.2012.04.006 | This paper presents a novel method for non-rigid registration of transrectal ultrasound and magnetic resonance prostate images based on a non-linear regularized framework of point correspondences obtained from a statistical measure of shape-contexts. The segmented prostate shapes are represented by shape-contexts and the Bhattacharyya distance between the shape representations is used to find the point correspondences between the 2D fixed and moving images. The registration method involves parametric estimation of the non-linear diffeomorphism between the multimodal images and has its basis in solving a set of non-linear equations of thin-plate splines. The solution is obtained as the least-squares solution of an over-determined system of non-linear equations constructed by integrating a set of non-linear functions over the fixed and moving images. However, this may not result in clinically acceptable transformations of the anatomical targets. Therefore, the regularized bending energy of the thin-plate splines along with the localization error of established correspondences should be included in the system of equations. The registration accuracies of the proposed method are evaluated in 20 pairs of prostate mid-gland ultrasound and magnetic resonance images. The results obtained in terms of Dice similarity coefficient show an average of 0.980±0.004, average 95% Hausdorff distance of 1.63±0.48 mm and mean target registration and target localization errors of 1.60±1.17 mm and 0.15±0.12 mm respectively. | Algorithms, Artificial Intelligence, Humans, Image Enhancement, Image Interpretation, Computer-Assisted, Magnetic Resonance Imaging, Male, Nonlinear Dynamics, Pattern Recognition, Automated, Prostatic Neoplasms, Reproducibility of Results, Sensitivity and Specificity, Subtraction Technique, Ultrasonography | null |
22,705,292 | 2012-12-03 | 2022-03-11 | 1532-3064 | Respiratory medicine | Epidemiological measures of childhood asthma: cross-sectional and longitudinal consistency. | Canova Cristina, Harris Jessica M, Mills Pamela, White Carol, Moffat Susan, Shread Lesley, Cullinan Paul | eng | G0601361 (Medical Research Council, United Kingdom) | Journal Article | null | IM | 22705292, S0954-6111(12)00192-8, 10.1016/j.rmed.2012.05.008 | Defining childhood asthma varies considerably, and the extent of agreement between various measures is not clearly understood in the absence of a recognized 'gold standard'. We compared different definitions of childhood asthma, identified characteristics that might have influenced their accuracy and an acquisition of an 'asthma' label in wheezy and treated children. | Adolescent, Asthma, Child, Child, Preschool, England, Epidemiologic Measurements, Epidemiologic Methods, Female, Humans, Infant, Male, Parents, Respiratory Sounds | null |
22,705,293 | 2012-12-03 | 2012-08-06 | 1532-3064 | Respiratory medicine | Overlap syndrome: additive effects of COPD on the cardiovascular damages in patients with OSA. | Shiina Kazuki, Tomiyama Hirofumi, Takata Yoshifumi, Yoshida Masanobu, Kato Kota, Nishihata Yosuke, Matsumoto Chisa, Odaira Mari, Saruhara Hirokazu, Hashimura Yuki, Usui Yasuhiro, Yamashina Akira | eng | null | Journal Article | Antihypertensive Agents | IM | 22705293, S0954-6111(12)00189-8, 10.1016/j.rmed.2012.05.006 | The chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) have been recently much focused as independent risks for cardiovascular disease. Furthermore, the complication of both has a worse prognosis compared with patients with only one of these diseases. However, the details of the underlying mechanisms of this worsened prognosis have not been clear. The cross-sectional study was conducted to examine whether the overlap of COPD augment the increase in arterial stiffness in subjects with OSA. If so, we examined the exaggeration of nocturnal hypoxemia and its related inflammation are related to this augmentation of increased arterial stiffness. In 524 male subjects with OSA diagnosed by polysomnography (apnea-hypopnea index >5/h) (52 ± 14 years old), the forced expiratory volume at 1 s/the forced vital capacity (FEV(1)/FVC) ratio, brachial-ankle pulse wave velocity (baPWV), blood C-reactive protein (CRP) and B-natriuretic peptide (BNP) levels were measured. The prevalence rate of COPD was 12% in this study subjects. Plasma BNP levels and the crude (median value, 17.2 vs. 14.1 m/s, p < 0.01) and adjusted value of baPWV were significantly higher in subjects with overlap syndrome than in those with OSA alone. However, parameters of nocturnal hypoxemia and serum CRP levels were similar between both groups. Thus, the overlap of COPD in patients with OSA augments increase in arterial stiffness without the exaggeration of nocturnal hypoxemia and inflammation. Even so, this augmentation may partially contribute to the increased cardiovascular risk in the overlap syndrome. | Antihypertensive Agents, Body Mass Index, Cause of Death, Cohort Studies, Humans, Hypertension, Male, Middle Aged, Polysomnography, Pulmonary Disease, Chronic Obstructive, Sleep Apnea, Obstructive | null |
22,705,294 | 2014-07-02 | 2021-10-21 | 1878-4216 | Progress in neuro-psychopharmacology & biological psychiatry | Anesthetic effects in Alzheimer transgenic mouse models. | Tang Junxia X, Eckenhoff Maryellen F | eng | R01 AG031742 (NIA NIH HHS, United States); NIH AG031742 (NIA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Anesthetics | IM | 22705294, S0278-5846(12)00142-X, 10.1016/j.pnpbp.2012.06.007, PMC3521854, NIHMS390802, 19372347, 17346857, 13243706, 11337275, 22964728, 19036972, 19820237, 19820240, 20102519, 20967591, 19786858, 21745760, 14993365, 10512285, 20179286, 20460993, 11140684, 19279139, 8810256, 20308791, 12895417, 22069482, 10932182, 9371838, 12859672 | Research has improved the diagnosis of Alzheimer's disease, and at earlier stages, but effective therapy continues to be elusive. Current effort is focused on delay. Environmental factors are thought to interact with genetics to modulate the progression of the disease, and one such environmental factor is exposure to general anesthetics. The possibility that some anesthetic effects have long-term consequences is of general interest and concern. The difficulty of studying a chronic, age-related disease in humans combined with the fact that anesthetics are rarely given without surgery, has led to a focus on animal models. Transgenic mouse models have been developed to mimic the hallmarks of Alzheimer's disease, including amyloid beta accumulation (plaque), neurofibrillary tangles, and cognitive dysfunction. While none of the models recapitulate the human disease with high fidelity, they allow a first look at anesthetic-Alzheimer interactions in a reasonable time frame. In studies found to date, none have concluded that anesthetics alone cause a significant change in cognitive decline, but rather an acceleration in Alzheimer neuropathology. Further studies are required to define the best anesthetic paradigm for our elderly population to mitigate changes in neuropathology and potentially cognition. | Alzheimer Disease, Anesthetics, Animals, Disease Models, Animal, Humans, Mice, Mice, Transgenic | 3xTgAD, AD, APP, APPswe, Alzheimer's disease, Amyloidopathy, Anesthetic sensitivity, Aβ, Desflurane, FAD, Isoflurane, MAC, NFTs, PSEN1, PSEN2, Presenilin-2, Sevoflurane, Tauopathy, amyloid beta, amyloid precursor protein, amyloid precursor protein with the Swedish mutation, familial Alzheimer's disease, minimum alveolar concentration, neurofibrillary tangles, presenilin-1, triple transgenic AD mouse model |
22,705,295 | 2013-01-21 | 2022-12-07 | 1878-4216 | Progress in neuro-psychopharmacology & biological psychiatry | Association of MB-COMT polymorphisms with schizophrenia-susceptibility and symptom severity in an African cohort. | Wright Galen E B, Niehaus Dana J H, van der Merwe Lize, Koen Liezl, Korkie Lundi J, Kinnear Craig J, Drögemöller Britt I, Warnich Louise | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Isoenzymes, Membrane Proteins, Catechol O-Methyltransferase | IM | 22705295, S0278-5846(12)00141-8, 10.1016/j.pnpbp.2012.06.006 | The catechol-O-methyltransferase (COMT) gene is an attractive schizophrenia candidate gene, encoding a catabolic dopamine enzyme. The enzyme exists as two distinct isoforms, with the membrane bound enzyme (i.e. MB-COMT) being predominantly expressed in the brain. Since African populations remain underrepresented in genetic/genomic research, we performed an association study to determine whether MB-COMT genetic variants are associated with schizophrenia-susceptibility and symptom severity in the South African Xhosa population. Fourteen candidate polymorphisms were selected by means of a literature search and in silico analyses and were subsequently genotyped in a cohort of 238 Xhosa schizophrenia patients and 240 healthy Xhosa controls. Genetic association was tested with schizophrenia-susceptibility as well as symptom severity within the patient group. Polymorphisms of interest were also analysed using functional assays. Two SNPs, rs2020917 (OR=0.54, 95% CI 0.37-0.79; P=0.0011) and rs737865 (OR=0.52, 95% CI 0.36-0.74; P=0.0002), in the P2 promoter region were significantly associated with schizophrenia as well as an increase (increase=11.2%, 95% CI 3.7%-19.2%; P=0.0031) in reporter gene expression. The minor alleles of these SNPs were underrepresented in the schizophrenia cohort, indicating a possible protective effect. The P2 region also formed part of a haplotype found to be associated with the severity of the negative symptoms of the disorder. The data generated by this study indicate that genetic variation of MB-COMT could be associated with schizophrenia and negative symptom severity in the Xhosa population and may therefore be one of the genomic loci contributing towards the disorder in the South African community. Future large-scale studies in other African schizophrenia populations are required to further elucidate the significance of these findings. | Adult, Alleles, Black People, Case-Control Studies, Catechol O-Methyltransferase, Cell Line, Female, Genetic Association Studies, Genetic Predisposition to Disease, Genotype, Haplotypes, Humans, Isoenzymes, Male, Membrane Proteins, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Psychiatric Status Rating Scales, Schizophrenia, Symptom Assessment | null |
22,705,296 | 2013-11-18 | 2022-03-21 | 1473-0502 | Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis | Clinical factors influencing corrected count increment. | Shastry Shamee, Chaudhary Rajendra | eng | null | Journal Article | null | null | 22705296, S1473-0502(12)00123-1, 10.1016/j.transci.2012.04.006 | Refractoriness to platelet transfusions is a common clinical problem. The present study was conducted to look into patient characteristics affecting the corrected count increment in a tertiary care referral hospital. A total of 161 aphaeresis platelet units were transfused to 40 patients with varied clinical diagnoses. The mean platelet increment was 17,789/mm(3). Median corrected count increment was 7344 and percentage platelet recovery was 22.82%. Logistic regression analysis revealed significant influence of splenomegaly and anti-platelet drugs on refractoriness. Fever, bleeding, sepsis, disseminated intravascular coagulation and cyclosporine use, though more common in the patients with refractoriness they were not statistically significant. | Adolescent, Adult, Aged, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Platelet Count, Platelet Transfusion, Plateletpheresis, Prospective Studies, Young Adult | null |
22,705,297 | 2012-11-07 | 2013-01-08 | 1532-2939 | The Journal of hospital infection | Skin sealant InteguSeal® has no impact on prevention of postoperative mediastinitis after cardiac surgery. | Waldow T, Szlapka M, Hensel J, Plötze K, Matschke K, Jatzwauk L | eng | null | Journal Article | Anti-Infective Agents, Local, Cyanoacrylates, Tissue Adhesives | IM | 22705297, S0195-6701(12)00145-4, 10.1016/j.jhin.2012.04.024 | Surgical site infections (SSIs) after median sternotomy represent a serious complication and a high potential risk for adverse clinical outcome after cardiac surgery. The antimicrobial skin sealant InteguSeal® was introduced as a novel tool in preventing development of SSI. | Aged, Anti-Infective Agents, Local, Cyanoacrylates, Humans, Incidence, Male, Mediastinitis, Middle Aged, Skin, Surgical Wound Infection, Thoracic Surgery, Tissue Adhesives, Treatment Outcome | null |
22,705,299 | 2012-12-24 | 2022-03-31 | 1526-3231 | Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association | Arthroscopic repair for posterior shoulder instability. | Lenart Brett A, Sherman Seth L, Mall Nathan A, Gochanour Eric, Twigg Stacy L, Nicholson Gregory P | eng | null | Journal Article | null | IM | 22705299, S0749-8063(12)00203-4, 10.1016/j.arthro.2012.03.011 | The purpose of this study was to evaluate outcomes of a consistent arthroscopic stabilization technique for recurrent posterior instability. | Adolescent, Adult, Arthroscopy, Female, Humans, Joint Instability, Male, Recurrence, Shoulder Dislocation, Shoulder Injuries, Shoulder Joint, Treatment Outcome, Young Adult | null |
22,705,298 | 2012-11-07 | 2013-11-21 | 1532-2939 | The Journal of hospital infection | In vivo evaluation of the persistant and residual antimicrobial properties of three hand-scrub and hand-rub regimes in a simulated surgical environment. | Beausoleil C M, Paulson D S, Bogert A, Lewis G S | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Alcohols, Disinfectants, chlorhexidine gluconate, Chlorhexidine | IM | 22705298, S0195-6701(12)00146-6, 10.1016/j.jhin.2012.05.003 | The use of antimicrobial formulations for disinfecting hands prior to surgery has been shown to reduce the incidence of surgical site infections. Such formulations must demonstrate an immediate reduction of microbial flora on the hands that persists while the hands are gloved. | Alcohols, Chlorhexidine, Colony Count, Microbial, Disinfectants, Hand Disinfection, Humans, Metagenome, Skin, Staphylococcus aureus | null |
22,705,300 | 2012-10-22 | 2013-11-21 | 1090-2104 | Biochemical and biophysical research communications | Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells. | Yu Yun, Shen Shao-Ming, Zhang Fei-Fei, Wu Zhao-Xia, Han Bin, Wang Li-Shun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | ANP32B protein, human, Nuclear Proteins, RNA, Small Interfering, Receptors, Retinoic Acid, Tretinoin | IM | 22705300, S0006-291X(12)01113-8, 10.1016/j.bbrc.2012.06.025 | The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B) is a member of a conserved superfamily of nuclear proteins whose functions are largely unknown. In our previous work, ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. In this work, we provided the first demonstration that ANP32B expression was down-regulated during differentiation induction of leukemic cells by all-trans retinoic acid (ATRA). Knockdown of ANP32B expression by specific shRNA enhanced ATRA-induced leukemic cell differentiation, while ectopic expression of ANP32B attenuated it, indicating an inhibitory role of ANP32B against leukemic cell differentiation. Furthermore, luciferase reporter assay revealed that ANP32B might exert this role through inhibiting the ATRA dependent transcriptional activity of retinoic acid receptor (RARα). These data will shed new insights into understanding the biological functions of ANP32B protein. | Apoptosis, Cell Line, Tumor, Down-Regulation, Gene Knockdown Techniques, Humans, Leukemia, Myeloid, Acute, Nuclear Proteins, RNA, Small Interfering, Receptors, Retinoic Acid, Signal Transduction, Tretinoin | null |
22,705,301 | 2012-10-22 | 2021-10-21 | 1090-2104 | Biochemical and biophysical research communications | β-Hydroxy-β-methylbutyrate (HMB) prevents dexamethasone-induced myotube atrophy. | Aversa Zaira, Alamdari Nima, Castillero Estibaliz, Muscaritoli Maurizio, Rossi Fanelli Filippo, Hasselgren Per-Olof | eng | R01 DK037908 (NIDDK NIH HHS, United States); R01 DK37908 (NIDDK NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Anti-Inflammatory Agents, Muscle Proteins, Tripartite Motif Proteins, Valerates, beta-hydroxyisovaleric acid, Dexamethasone, Fbxo32 protein, mouse, SKP Cullin F-Box Protein Ligases, Trim63 protein, mouse, Ubiquitin-Protein Ligases | IM | 22705301, S0006-291X(12)01117-5, 10.1016/j.bbrc.2012.06.029, PMC3399026, NIHMS387008, 15574784, 8791198, 21381078, 3511355, 20821267, 11679633, 19404720, 18372227, 18854427, 11717410, 15665304, 10917905, 21871000, 18840762, 20473154, 17609254, 21184031, 8567953, 8941534, 16149047, 8000653, 10850936, 18615595, 15896432, 9788663, 4019954, 19211028, 22166204, 11975938, 11435528, 20725872, 21402749 | High levels of glucocorticoids result in muscle wasting and weakness. β-hydroxy-β-methylbutyrate (HMB) attenuates the loss of muscle mass in various catabolic conditions but the influence of HMB on glucocorticoid-induced muscle atrophy is not known. We tested the hypothesis that HMB prevents dexamethasone-induced atrophy in cultured myotubes. Treatment of cultured L6 myotubes with dexamethasone resulted in increased protein degradation and expression of atrogin-1 and MuRF1, decreased protein synthesis and reduced myotube size. All of these effects of dexamethasone were attenuated by HMB. Additional experiments provided evidence that the inhibitory effects of HMB on dexamethasone-induced increase in protein degradation and decrease in protein synthesis were regulated by p38/MAPK- and PI3K/Akt-dependent cell signaling, respectively. The present results suggest that glucocorticoid-induced muscle wasting can be prevented by HMB. | Animals, Anti-Inflammatory Agents, Cell Line, Dexamethasone, Muscle Fibers, Skeletal, Muscle Proteins, Muscle Weakness, Muscular Atrophy, Protein Biosynthesis, Proteolysis, Rats, SKP Cullin F-Box Protein Ligases, Tripartite Motif Proteins, Ubiquitin-Protein Ligases, Valerates | null |
22,705,302 | 2012-10-22 | 2013-11-21 | 1090-2104 | Biochemical and biophysical research communications | The FeoA protein is necessary for the FeoB transporter to import ferrous iron. | Kim Hyunkeun, Lee Hwiseop, Shin Dongwoo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cation Transport Proteins, Cations, Divalent, Ferrous Compounds, Iron | IM | 22705302, S0006-291X(12)01115-1, 10.1016/j.bbrc.2012.06.027 | In many bacterial feo loci, the feoA gene is associated with the feoB gene. While the feoB-encoded FeoB protein has been demonstrated as a ferrous iron [Fe(II)] transporter, the function of the feoA gene product, FeoA, is unknown. In the present study, we report that the FeoA protein interacts with the FeoB Fe(II) transporter, which is required for FeoB-mediated Fe(II) uptake in Salmonella enterica. Iron uptake assay revealed that in the absence of FeoA, FeoB import of Fe(II) is impaired. Bacterial two-hybrid assay determined that the FeoA protein directly and specifically binds to the FeoB transporter in vivo. This FeoA-FeoB interaction appeared necessary for FeoB-mediated Fe(II) uptake because Salmonella expressing the mutant FeoA that cannot interact with FeoB failed to uptake Fe(II) via the FeoB transporter. Finally, we showed that the FeoA protein does not affect expression of the FeoB transporter per se. | Cation Transport Proteins, Cations, Divalent, Ferrous Compounds, Iron, Mutation, Salmonella typhimurium, Two-Hybrid System Techniques | null |
22,705,303 | 2012-10-22 | 2012-07-17 | 1090-2104 | Biochemical and biophysical research communications | Oral administration of FAK inhibitor TAE226 inhibits the progression of peritoneal dissemination of colorectal cancer. | Hao Hui-fang, Takaoka Munenori, Bao Xiao-hong, Wang Zhi-gang, Tomono Yasuko, Sakurama Kazufumi, Ohara Toshiaki, Fukazawa Takuya, Yamatsuji Tomoki, Fujiwara Toshiyoshi, Naomoto Yoshio | eng | null | Journal Article | Morpholines, Protein Kinase Inhibitors, RNA, Small Interfering, TAE226, Focal Adhesion Protein-Tyrosine Kinases | IM | 22705303, S0006-291X(12)01118-7, 10.1016/j.bbrc.2012.06.030 | Peritoneal dissemination is one of the most terrible types of colorectal cancer progression. Focal adhesion kinase (FAK) plays a crucial role in the biological processes of cancer, such as cell attachment, migration, proliferation and survival, all of which are essential for the progression of peritoneal dissemination. Since we and other groups have reported that the inhibition of FAK activity exhibited a potent anticancer effect in several cancer models, we hypothesized that TAE226, a novel ATP-competitive tyrosine kinase inhibitor designed to target FAK, can prevent the occurrence and progression of peritoneal dissemination. In vitro, TAE226 greatly inhibited the proliferation and migration of HCT116 colon cancer cells, while their adhesion on the matrix surface was minimally inhibited when FAK activity and expression was suppressed by TAE226 and siRNA. In vivo, when HCT116 cells were intraperitoneally inoculated in mice, the cells could attach to the peritoneum and begin to grow within 24 h regardless of the pretreatment of cells with TAE226 or FAK-siRNA, suggesting that FAK is not essential, at least for the initial integrin-matrix contact. Interestingly, the treatment of mice before and after inoculation significantly suppressed cell attachment to the peritoneum. Furthermore, oral administration of TAE226 greatly reduced the size of disseminated tumors and prolonged survival in tumor-bearing mice. Taken together, a possible strategy for inhibiting peritoneal dissemination by targeting FAK with TAE226 appears to be applicable through anti-proliferative and anti-invasion/anti-migration mechanisms. | Administration, Oral, Animals, Cell Proliferation, Colorectal Neoplasms, Disease Progression, Focal Adhesion Protein-Tyrosine Kinases, HCT116 Cells, Humans, Male, Mice, Mice, Inbred BALB C, Morpholines, Neoplasm Invasiveness, Peritoneum, Protein Kinase Inhibitors, RNA, Small Interfering | null |
22,705,304 | 2012-11-06 | 2018-12-11 | 1090-2104 | Biochemical and biophysical research communications | miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer. | Li Qiaoyan, Zhu Fufan, Chen Puxiang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | 3' Untranslated Regions, Claudins, Homeodomain Proteins, HoxB3 protein, human, MIRN218 microRNA, human, MIRN7 microRNA, human, MicroRNAs, RASSF1 protein, human, Tumor Suppressor Proteins, claudin 6 | IM | 22705304, S0006-291X(12)01116-3, 10.1016/j.bbrc.2012.06.028 | Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy. | 3' Untranslated Regions, Breast Neoplasms, Cell Line, Tumor, Cell Proliferation, Claudins, Epigenesis, Genetic, Gene Expression Regulation, Neoplastic, Homeodomain Proteins, Humans, MicroRNAs, Tumor Suppressor Proteins | null |
22,705,305 | 2012-10-22 | 2019-12-10 | 1090-2104 | Biochemical and biophysical research communications | The role of histone chaperones in osteoblastic differentiation of C2C12 myoblasts. | Song Tae-Yang, Yang Jae-Hyun, Park Jin Young, Song Yunkyoung, Han Jeung-Whan, Youn Hong-Duk, Cho Eun-Jung | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Asf1a protein, mouse, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Hira protein, mouse, Histone Chaperones, Histones, Molecular Chaperones, MyoD Protein, Myogenin, RNA, Small Interfering, Transcription Factors, Osteocalcin, Alkaline Phosphatase | IM | 22705305, S0006-291X(12)01114-X, 10.1016/j.bbrc.2012.06.026 | Cellular differentiation is a process in which the cells gain a more specialized shape, metabolism, and function. These cellular changes are accompanied by dynamic changes in gene expression programs. In most cases, DNA methylation, histone modification, and variant histones drive the epigenetic transition that reprograms the gene expression. Histone chaperones, HIRA and Asf1a, have a role for cellular differentiation by deposition of one of variant histones, H3.3, during myogenesis of murine C2C12 cells. In this study, we accessed the roles of histone chaperones and histone H3.3 in osteoblastic conversion of C2C12 myoblasts and compared their roles with those for myogenic differentiation. The unbiased analysis of the expression pattern of histone chaperones and variant histones proposed their uncommon contribution to each pathway. HIRA and Asf1a decreased to ∼50% and further diminished during differentiation into osteoblasts, while they were maintained during differentiation into myotubes. HIRA, Asf1a, and H3.3 were indispensable for expression of cell type-specific genes during conversion into osteoblasts or myotubes. RNA interference analysis indicated that histone chaperones and H3.3 were required for early steps of osteoblastic differentiation. Our results suggest that histone chaperones and variant histones might be differentially required for the distinct phases of differentiation pathway. | Alkaline Phosphatase, Animals, Cell Cycle Proteins, Cell Differentiation, Cell Line, Chromosomal Proteins, Non-Histone, Gene Expression Regulation, Developmental, Histone Chaperones, Histones, Mice, Molecular Chaperones, Muscle Development, Muscle Fibers, Skeletal, MyoD Protein, Myoblasts, Myogenin, Osteoblasts, Osteocalcin, Osteogenesis, RNA, Small Interfering, Transcription Factors | null |
22,705,306 | 2013-03-25 | 2016-11-25 | 1873-6807 | Body image | Who is most likely to fat talk? A social comparison perspective. | Corning Alexandra F, Gondoli Dawn M | eng | null | Journal Article | null | IM | 22705306, S1740-1445(12)00071-X, 10.1016/j.bodyim.2012.05.004 | Negative talk about food, weight, or the body that occurs commonly among women--fat talk--is gaining increasing attention. Whereas its negative eating pathology and body esteem correlates have received continued empirical validation, what is not yet known is who is most likely to fat talk. We propose that social comparison processes underlie and motivate much of fat talk. In a sample of 143 college women, we found evidence for the role of social comparison. First, having a stronger tendency to socially compare directly predicts fat talk. Second, as a woman's body image concerns increase, her likelihood of engaging in fat talk increases, and this is intensified if she has a greater tendency to socially compare. Finally, social comparison propensity begins to exert its exacerbating effects at surprisingly low levels of body image concern. Results are discussed in terms of the advantages of using a social comparison perspective to better understand fat talk. | Adolescent, Attitude, Body Image, Body Mass Index, Body Weight, Drive, Feeding Behavior, Female, Gender Identity, Humans, Ideal Body Weight, Motivation, Obesity, Overweight, Social Desirability, Social Values, Students, Thinness, Verbal Behavior, Young Adult | null |
22,705,307 | 2014-07-17 | 2022-03-11 | 1873-507X | Physiology & behavior | Daily profile in two circadian markers "melatonin and cortisol" and associations with metabolic syndrome components. | Corbalán-Tutau Dolores, Madrid Juan Antonio, Nicolás Francisco, Garaulet Marta | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Melatonin, Hydrocortisone | IM | 22705307, S0031-9384(12)00229-6, 10.1016/j.physbeh.2012.06.005 | The aim of the present work was to investigate associations in circadian markers, melatonin (MT) and cortisol, with metabolic syndrome (MetS) parameters, and with leptin, adiponectin and ghrelin plasma values. | Adult, Anthropometry, Circadian Rhythm, Female, Humans, Hydrocortisone, Linear Models, Melatonin, Metabolic Diseases, Middle Aged, Radioimmunoassay, Saliva | Adiponectin, Cortisol, Ghrelin, Leptin, Melatonin, Metabolic syndrome |
22,705,309 | 2013-01-17 | 2012-11-19 | 1879-0631 | Life sciences | Evidence for the accumulation of Abeta immunoreactive material in the human brain and in transgenic animal models. | Cuello A Claudio, Allard Simon, Ferretti Maria Teresa | eng | null | Journal Article, Review | Amyloid beta-Peptides, Intracellular Signaling Peptides and Proteins | IM | 22705309, S0024-3205(12)00294-9, 10.1016/j.lfs.2012.05.020 | In this review we highlight the evidence for an intracellular origin of Abeta (Aβ) amyloid peptides as well as the observations for a pathological accumulation of these peptides in Alzheimer's disease and Down syndrome, as well as in transgenic animal models. We deliberate on the controversy as to whether the intracellular Aβ immunoreactive material is simply an accumulation of unprocessed full length amyloid precursor protein (APP) or a mix of processed APP fragments including Aβ. Finally, we discuss the possible pathological significance of these intracellular APP fragments and the expected future research directions regarding this thought-provoking problem. | Alzheimer Disease, Amyloid beta-Peptides, Animals, Animals, Genetically Modified, Brain, Down Syndrome, Humans, Intracellular Signaling Peptides and Proteins, Mice, Rats | null |
22,705,310 | 2013-04-17 | 2024-05-12 | 1879-0631 | Life sciences | The role of fatty acid amide hydrolase inhibition in nicotine reward and dependence. | Muldoon Pretal P, Lichtman Aron H, Parsons Loren H, Damaj M Imad | eng | P50 DA005274 (NIDA NIH HHS, United States); DA009789 (NIDA NIH HHS, United States); P01 DA009789 (NIDA NIH HHS, United States); RC2 DA028845 (NIDA NIH HHS, United States); DA-05274 (NIDA NIH HHS, United States); DA017259 (NIDA NIH HHS, United States); P01 DA017259 (NIDA NIH HHS, United States); DA005274 (NIDA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Endocannabinoids, Enzyme Inhibitors, Nicotinic Agonists, Nicotine, Amidohydrolases, fatty-acid amide hydrolase | IM | 22705310, S0024-3205(12)00289-5, 10.1016/j.lfs.2012.05.015, PMC3477273, NIHMS386636, 9460749, 15292905, 19091987, 10731620, 19484221, 21557729, 16818490, 11470906, 15579492, 18288264, 18194436, 12955147, 18814866, 15157693, 15254019, 19389627, 17210800, 12394421, 21505060, 21437438, 2308954, 21412887, 17709120, 8900284, 20590555, 12393235, 1792691, 16224028, 21521420, 15925402, 2899326, 15486497, 15548217, 12432948, 17217969, 11512050, 19637155, 17628012, 12655057, 16596771, 22140525, 16834756, 17040625, 1470919, 15194878, 11818483, 21785434, 14595399, 12384171, 15661626, 3788613, 11815392, 16416159, 2573910, 16820547, 20729846, 8717040, 17109648, 21501143, 18429637, 18451315, 12761211, 15229230, 21763761, 15550444, 16298297, 16204639, 20477753, 9473676, 2165569 | The endogenous cannabinoid anandamide (AEA) exerts the majority of its effects at CB1 and CB2 receptors and is degraded by fatty acid amide hydrolase (FAAH). FAAH KO mice and animals treated with FAAH inhibitors are impaired in their ability to hydrolyze AEA and other non-cannabinoid lipid signaling molecules, such as oleoylethanolamide (OEA) and palmitoylethanolamide (PEA). AEA and these other substrates activate non-cannabinoid receptor systems, including TRPV1 and PPAR-α receptors. In this mini review, we describe the functional consequences of FAAH inhibition on nicotine reward and dependence as well as the underlying endocannabinoid and non-cannabinoid receptor systems mediating these effects. Interestingly, FAAH inhibition seems to mediate nicotine dependence differently in mice and rats. Indeed, pharmacological and genetic FAAH disruption in mice enhances nicotine reward and withdrawal. However, in rats, pharmacological blockade of FAAH significantly inhibits nicotine reward and has no effect in nicotine withdrawal. Studies suggest that non-cannabinoid mechanisms may play a role in these species differences. | Amidohydrolases, Animals, Endocannabinoids, Enzyme Inhibitors, Humans, Mice, Nicotine, Nicotinic Agonists, Rats, Reward, Substance Withdrawal Syndrome, Tobacco Use Disorder | null |
22,705,313 | 2012-12-10 | 2013-11-21 | 1873-6815 | Experimental gerontology | Influence of large molecular polymeric pigments isolated from fermented Zijuan tea on the activity of key enzymes involved in lipid metabolism in rat. | Wang Qiu-Ping, Peng Chun-Xiu, Gao Bin, Gong Jia-Shun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acyl Coenzyme A, Enzymes, Pigments, Biological, Plant Extracts, RNA, Messenger, Tea, 3-hydroxy-3-methylglutaryl-coenzyme A, Cholesterol, Phosphatidylcholine-Sterol O-Acyltransferase, Sterol Esterase, Lipoprotein Lipase | IM | 22705313, S0531-5565(12)00155-6, 10.1016/j.exger.2012.06.002 | Influence of large molecular polymeric pigments (LMPP) isolated from fermented Zijuan tea on the activity and mRNA expression of key enzymes involved in lipid metabolism in rat was explored. The results show that intragastric infusion of high-dose LMPP (1.215 g/kg body weight) effectively suppressed the elevation in TC and LDL-C (p<0.05), and prevented the reduction in HDL-C (p<0.05), compared with the hyperlipidemia model group. LMPP significantly enhanced the activity of HL and HSL, and increased the HSL mRNA expression in the liver tissue and adipose tissue. High-LMPP treatment significantly reduced the HMG-CoA reductase expression by 56.5% in the liver compared with hyperlipidemia model group. In contrast, LDL-R expression was increased by 120% in the presence of high-LMPP treatment. These results suggest that LMPP have the hypolipidemic effect to some extent and significantly enhance HSL mRNA expression in the liver and adipose tissue, thereby increasing HSL activity in rat. | Acyl Coenzyme A, Adipose Tissue, Animals, Cholesterol, Enzymes, Fermentation, Hyperlipidemias, Lipid Metabolism, Lipoprotein Lipase, Liver, Male, Phosphatidylcholine-Sterol O-Acyltransferase, Pigments, Biological, Plant Extracts, RNA, Messenger, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Sterol Esterase, Tea | null |
22,705,312 | 2013-04-04 | 2022-03-16 | 1873-6815 | Experimental gerontology | The need to unify neuropathological assessments of vascular alterations in the ageing brain: multicentre survey by the BrainNet Europe consortium. | Alafuzoff Irina, Gelpi Ellen, Al-Sarraj Safa, Arzberger Thomas, Attems Johannes, Bodi Istvan, Bogdanovic Nenad, Budka Herbert, Bugiani Orso, Englund Elisabet, Ferrer Isidro, Gentleman Stephen, Giaccone Giorgio, Graeber Manuel B, Hortobagyi Tibor, Höftberger Romana, Ironside James W, Jellinger Kurt, Kavantzas Nikolaos, King Andrew, Korkolopoulou Penelope, Kovács Gábor G, Meyronet David, Monoranu Camelia, Parchi Piero, Patsouris Efstratios, Roggendorf Wolfgang, Rozemuller Annemieke, Seilhean Danielle, Streichenberger Nathalie, Thal Dietmar R, Wharton Stephen B, Kretzschmar Hans | eng | G0901945 (Medical Research Council, United Kingdom); G1100540 (Medical Research Council, United Kingdom); G0900652 (Medical Research Council, United Kingdom); G0502157 (Medical Research Council, United Kingdom); G0900580 (Medical Research Council, United Kingdom); G0600953 (Medical Research Council, United Kingdom); G0400074 (Medical Research Council, United Kingdom) | Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't | null | IM | 22705312, S0531-5565(12)00154-4, 10.1016/j.exger.2012.06.001 | Here, we summarise the results after carrying out a large survey regarding the assessment of vascular alterations, both vessel changes and vascular lesions in an inter-laboratory setting. In total, 32 neuropathologists from 22 centres, most being members of BrainNet Europe (BNE), participated by filling out a questionnaire with emphasis on assessment of common vascular alterations seen in the brains of aged subjects. A certain level of harmonisation has been reached among BNE members regarding sectioning of the brain, harvesting of brain tissue for histology and staining used when compared to the survey carried out in 2006 by Pantoni and colleagues. The most significant variability was seen regarding the assessment of severity and of clinical significance of vascular alterations. Two strategies have recently been recommended regarding the assessment of vascular alterations in aged and demented subjects. The National Institute on Aging - Alzheimer's Association (NIA-AA) recommends the assessment of hippocampal sclerosis, vascular brain injury and microvascular lesions in 12 regions. Although this strategy will be easy to follow, the recommendations do not inform how the load of observed alterations should be assessed and when the observed lesions are of significance. Deramecourt and his colleagues recommend an assessment and semiquantitative grading of various pathologies in 4 brain regions. This strategy yielded a total score of 0 to 20 as an estimate of pathology load. It is, however, not clear which score is considered to be of clinical significance. Furthermore, in several BNE trials the semiquantitative assessment has yielded poor agreement rates; an observation that might negatively influence the strategy proposed by Deramecourt and his colleagues. In line with NIA-AA, a dichotomised approach of easily recognisable lesions in a standardised set of brain regions harvested for neuropathological assessment and applying reproducible sampling and staining strategies is recommended by BNE. However, a simple strategy regarding assessment of load of alteration is urgently needed to yield reproducible, and at the same time, comparable results between centres. | Aging, Brain, Cerebrovascular Circulation, Cerebrovascular Disorders, Dementia, Humans, Reproducibility of Results, Severity of Illness Index, Specimen Handling, Staining and Labeling, Surveys and Questionnaires | null |
22,705,314 | 2012-11-20 | 2012-07-23 | 1872-9428 | Molecular and biochemical parasitology | Novel RuvB nuclear ATPase is specific to intraerythrocytic mitosis during schizogony of Plasmodium falciparum. | Ahmad Moaz, Singh Shailja, Afrin Farhat, Tuteja Renu | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Nuclear Proteins, Protozoan Proteins, Adenosine Triphosphatases | IM | 22705314, S0166-6851(12)00174-0, 10.1016/j.molbiopara.2012.06.002 | RuvB protein belongs to AAA+ family of enzymes involved in diverse cellular activities. In addition to the annotated two RuvB proteins in Plasmodium falciparum database, we report that a third RuvB protein is also present. The amino acid sequence analysis has revealed that P. falciparum RuvB3 (PfRuvB3) possesses Walker motif A, Walker motif B, sensor I and sensor II conserved motifs similar to yeast and human RuvB like proteins. The phylogenetic analysis revealed that PfRuvB3 is closely related to yeast RuvB like proteins which are essential for the survival of yeast. The biochemical characterization of recombinant PfRuvB3 confirms its ssDNA dependent ATPase activity. Using the truncated derivatives we show that Walker motif A is essential for the enzymatic activity of PfRuvB3. Using the immunodepletion assays we further show that the ATPase activity is attributable to PfRuvB3 protein. The endogenous P. falciparum RuvB3 contains the characteristic ATPase and some DNA helicase activities. The confocal microscopy analysis showed that this protein is mainly expressed during intraerythrocytic schizont stages of the parasite and is localized to the nuclear region. Once merozoite comes out from schizont, PfRuvB3 protein distinctly relocalized to the subnuclear region. The co-localization studies with a nucleolar marker PfNop1 further suggest that in P. falciparum RuvB3 localizes into a discrete nuclear compartment. On the basis of these studies it can be speculated that P. falciparum RuvB3 is most likely required for intraerythrocytic schizogony. | Adenosine Triphosphatases, Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Nucleus, Enzyme Activation, Erythrocytes, Gene Expression Regulation, Microscopy, Confocal, Mitosis, Molecular Sequence Data, Nuclear Proteins, Phylogeny, Plasmodium falciparum, Protein Transport, Protozoan Proteins, Schizonts, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Substrate Specificity | null |
22,705,315 | 2012-11-20 | 2015-11-19 | 1872-9428 | Molecular and biochemical parasitology | Expansion of experimental genetics approaches for Plasmodium berghei with versatile transfection vectors. | Kooij Taco W A, Rauch Manuel M, Matuschewski Kai | eng | null | Journal Article | Biomarkers, Green Fluorescent Proteins | IM | 22705315, S0166-6851(12)00173-9, 10.1016/j.molbiopara.2012.06.001 | Experimental reverse genetic approaches have proven powerful in the study of the biology of the malaria parasite. The murine malaria model parasite Plasmodium berghei is the genetically most amendable Plasmodium species and allows full access to the entire life cycle in vivo. Here, we describe a next-generation, highly versatile transfection vector set that facilitates advancing experimental genetic strategies towards a genome-wide scale. Through 36 consecutive cloning and 17 subcloning steps an optimized vector set was generated from the standard transfection plasmid. These targeting vectors, collectively referred to as the Berghei Adaptable Transfection (pBAT) plasmids, contain key elements that permit recycling of the drug-selectable cassette, robust green fluorescent labelling of recombinant parasites, carboxy-terminal tagging of target proteins with a red fluorescent-epitope tag fusion, and expression of heterologous genes. The vectors were further optimized for small size, versatile restriction endonuclease recognition sites and potential exchange of individual vector elements. We show that stable integration into a transgene expression site, an intergenic locus at a synteny breakpoint on P. berghei chromosome 6, is phenotypically silent and generated a bright green fluorescent parasite line for imaging applications. We provide an example, P. berghei actin 2, for targeted gene deletion and illustrate that the positive selection marker can be recycled, thereby permitting multiple rounds of genetic manipulations. We propose that the vectors described herein will greatly facilitate functional assignment to predicted and orphan Plasmodium gene models by multiple experimental genetics approaches. | Animals, Biomarkers, Chromosomes, Cloning, Molecular, Gene Deletion, Genetic Engineering, Genetic Loci, Genetic Vectors, Genome, Protozoan, Green Fluorescent Proteins, Life Cycle Stages, Male, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Organisms, Genetically Modified, Phenotype, Plasmids, Plasmodium berghei, Promoter Regions, Genetic, Synteny, Transfection, Transgenes | null |
22,705,311 | 2013-12-04 | 2021-10-21 | 1095-9130 | Methods (San Diego, Calif.) | Two new monoclonal antibodies for biochemical and flow cytometric analyses of human interferon regulatory factor-3 activation, turnover, and depletion. | Rustagi Arjun, Doehle Brian P, McElrath M Juliana, Gale Michael | eng | U19 AI083019 (NIAID NIH HHS, United States); R01 AI060389 (NIAID NIH HHS, United States); R01AI060389 (NIAID NIH HHS, United States); T32 AI007140 (NIAID NIH HHS, United States); R01 DA024563 (NIDA NIH HHS, United States); 5T32GM007266-34 (NIGMS NIH HHS, United States); R01DA024563 (NIDA NIH HHS, United States); U19AI08319 (NIAID NIH HHS, United States); T32 AI07140-32 (NIAID NIH HHS, United States); T32 GM007266 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Antibodies, Monoclonal, Humanized, IRF3 protein, human, Interferon Regulatory Factor-3 | IM | 22705311, S1046-2023(12)00134-X, 10.1016/j.ymeth.2012.05.011, PMC3568209, NIHMS386645, 9463386, 20890285, 16413926, 12702807, 14555996, 16155125, 19622906, 10082512, 20511230, 18094709, 17942531, 17846036, 21379343, 10585427, 9891032, 20303872, 17395582, 9971817, 11314014, 18082865, 16699525, 21411754, 18711448, 17526488, 18548002, 19706707, 16971436, 19038458, 19158675, 16731946, 8524823, 11070172, 19151095, 12702806, 11318879, 19609254, 15708988, 9566918, 21947006, 9861020, 15741273, 10954535, 19631370, 16177806, 16585524 | Interferon regulatory factor-3 (IRF-3) is a master transcription factor that drives the host intracellular innate immune response to virus infection. The importance of IRF-3 in innate immune responses is highlighted by the fact that pathogenic viruses have developed strategies for antagonism of IRF-3. Several tools exist for evaluation of viral regulation of IRF-3 activation and function, but high-quality monoclonal antibodies that mark the differential activation states of human IRF-3 are lacking. To study IRF-3 activation, turnover, and depletion in a high-throughput manner in the context of virus infection, we have developed two new monoclonal antibodies to human IRF-3. These antibodies detect IRF-3 in virus-infected cells in a wide variety of assays and provide a new tool to study virus-host interactions and innate immune signaling. | Amino Acid Sequence, Animals, Antibodies, Monoclonal, Humanized, Cell Line, Tumor, Cells, Cultured, Flow Cytometry, HeLa Cells, Host-Pathogen Interactions, Humans, Interferon Regulatory Factor-3, Mice, Molecular Sequence Data | null |
22,705,316 | 2013-03-05 | 2022-12-07 | 1532-6500 | Journal of shoulder and elbow surgery | Radial head reconstruction versus replacement in the treatment of terrible triad injuries of the elbow. | Leigh Warren B, Ball Craig M | eng | null | Comparative Study, Journal Article | null | IM | 22705316, S1058-2746(12)00114-0, 10.1016/j.jse.2012.03.005 | Dislocation of the elbow with associated fractures of the radial head and the coronoid process of the ulna have been referred to as the terrible triad of the elbow because of the difficulties in treating this injury and the poor outcomes. | Adult, Aged, Collateral Ligaments, Elbow Joint, Female, Follow-Up Studies, Fracture Fixation, Internal, Humans, Joint Dislocations, Male, Middle Aged, Radiography, Radius Fractures, Range of Motion, Articular, Time Factors, Trauma Severity Indices, Treatment Outcome, Ulna Fractures, Young Adult, Elbow Injuries | null |
22,705,317 | 2013-05-20 | 2022-04-10 | 1532-6500 | Journal of shoulder and elbow surgery | Improvement in shoulder rotation in complex shoulder fractures treated by reverse shoulder arthroplasty. | Gallinet David, Adam Antoine, Gasse Nicolas, Rochet Severin, Obert Laurent | eng | null | Journal Article | null | IM | 22705317, S1058-2746(12)00120-6, 10.1016/j.jse.2012.03.011 | Reverse shoulder arthroplasty in complex shoulder fractures is now a common practice. Unfortunately, loss of rotation is observed when tuberosity excision is used, impairing function and patient satisfaction. The purpose of this study was to evaluate the advantage of tuberosity repair in terms of the functional result. | Aged, Aged, 80 and over, Arthroplasty, Case-Control Studies, Female, Humans, Male, Range of Motion, Articular, Retrospective Studies, Rotation, Shoulder Fractures, Shoulder Joint, Treatment Outcome | null |
22,705,308 | 2013-05-07 | 2012-11-05 | 1527-3288 | Heart & lung : the journal of critical care | Viridans streptococcal (Streptococcus mitis) biosynthetic aortic prosthetic valve endocarditis (PVE) complicated by complete heart block and paravalvular abscess. | Alkhatib Basil, Schoch Paul E, Cunha Burke A | eng | null | Case Reports, Journal Article | null | IM | 22705308, S0147-9563(12)00201-4, 10.1016/j.hrtlng.2012.05.002 | Prosthetic valve endocarditis (PVE) may be classified clinically as early (<60 days) or late (>60 days) post-valve replacement PVE. The pathogens of early versus late PVE differ in type and virulence. Early PVE pathogens are virulent, for example, Pseudomonas aeruginosa and Staphylococcus aureus. Late PVE pathogens resemble those of subacute bacterial endocarditis and are due to relatively avirulent and noninvasive organisms, for example, viridans streptococci. Viridans streptococci vary in their invasiveness and abscess potential. Myocardial abscess and complete heart block are rare complications of late PVE due to viridans streptococci. We present an unusual case of Streptococcus mitis late aortic PVE complicated by aortic root abscess, myocardial abscess, and complete heart block. | Abscess, Aged, Aortic Valve, Diagnosis, Differential, Endocarditis, Bacterial, Heart Block, Heart Valve Prosthesis, Humans, Male, Prosthesis-Related Infections, Streptococcal Infections, Streptococcus mitis | null |
22,705,318 | 2013-03-05 | 2012-09-18 | 1476-5411 | Contact lens & anterior eye : the journal of the British Contact Lens Association | Effect of three different liposomal eye sprays on ocular comfort and tear film. | Pult Heiko, Gill Felicity, Riede-Pult Britta H | eng | null | Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | Liposomes, Ophthalmic Solutions | IM | 22705318, S1367-0484(12)00056-2, 10.1016/j.clae.2012.05.003 | To evaluate the effect of three different liposomal eye sprays on ocular comfort and tear film stability. | Double-Blind Method, Dry Eye Syndromes, Eye Pain, Female, Humans, Liposomes, Male, Middle Aged, Ophthalmic Solutions, Patient Satisfaction, Tears, Treatment Outcome | null |
22,705,319 | 2013-05-23 | 2016-05-18 | 1876-7737 | Journal of proteomics | BCR/ABL modulates protein phosphorylation associated with the etoposide-induced DNA damage response. | Griaud François, Williamson Andrew J K, Taylor Samuel, Potier David N, Spooncer Elaine, Pierce Andrew, Whetton Anthony D | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, Phytogenic, Hemgn protein, mouse, Nuclear Proteins, Etoposide, Protein-Tyrosine Kinases, Fusion Proteins, bcr-abl | IM | 22705319, S1874-3919(12)00427-7, 10.1016/j.jprot.2012.06.003 | BCR/ABL expression is the key characteristic of chronic myeloid leukaemia (CML). The progression of CML is associated with genomic instability. Systematic analysis of DNA damage signalling in the presence of BCR/ABL thus offers opportunities to identify mechanisms of leukaemic progression. We therefore undertook a quantitative phosphoproteomics study to test whether BCR/ABL expression could globally affect the response to genotoxic stress signalling. Etoposide-induced DNA damage was chosen and the concentration and time of exposure determined such that apoptosis was not associated with the study. More than 1100 phosphoentities were identified. BCR/ABL was shown to significantly alter the response to etoposide in many cases. These include sites on MDC1, a key component of DNA repair, and Hemogen. Hemogen is a transcriptional target of HOXB4 and GATA1, two transcription factors involved in haemopoietic development, and is overexpressed in acute myeloid leukaemia. To validate Hemogen phosphorylation, absolute quantification using an isotopomeric standard and selected reaction monitoring was performed. This revealed a strong correlation with isobaric tagging data and offering quantification at about 10 fmol per million cells. Furthermore we found that multiple protein phosphorylation changes mediated by BCR/ABL were connected to the increased activation of NFκB, a key survival transcription factor, after etoposide exposure. | Animals, Antineoplastic Agents, Phytogenic, Apoptosis, Cell Line, Tumor, DNA Damage, Etoposide, Fusion Proteins, bcr-abl, Gene Expression Regulation, Leukemic, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Mice, Nuclear Proteins, Phosphorylation, Protein-Tyrosine Kinases | null |
22,705,321 | 2012-12-04 | 2022-03-10 | 1876-7737 | Journal of proteomics | Proteomic analysis of secreted protein induced by a component of prey in pitcher fluid of the carnivorous plant Nepenthes alata. | Hatano Naoya, Hamada Tatsuro | eng | null | Journal Article | Plant Proteins | IM | 22705321, S1874-3919(12)00429-0, 10.1016/j.jprot.2012.05.048 | The Nepenthes species are carnivorous plants that have evolved a specialized leaf organ, the 'pitcher', to attract, capture, and digest insects. The digested insects provide nutrients for growth, allowing these plants to grow even in poor soil. Several proteins have been identified in the pitcher fluid, including aspartic proteases (nepenthesin I and II) and pathogenesis-related (PR) proteins (β-1,3-glucanase, class IV chitinase, and thaumatin-like protein). In this study, we collected and concentrated pitcher fluid to identify minor proteins. In addition, we tried to identify the protein secreted in response to trapping the insect. To make a similar situation in which the insect falls into the pitcher, chitin which was a major component of the insect exoskeleton was added to the fluid in the pitcher. Three PR proteins, class III peroxidase (Prx), β-1,3-glucanase, and class III chitinase, were newly identified. Prx was induced after the addition of chitin to the pitcher fluid. Proteins in the pitcher fluid of the carnivorous plant Nepenthes alata probably have two roles in nutrient supply: digestion of prey and the antibacterial effect. These results suggest that the system for digesting prey has evolved from the defense system against pathogens in the carnivorous plant Nepenthes. | Animals, Insecta, Magnoliopsida, Plant Proteins, Proteomics | null |
22,705,320 | 2013-05-03 | 2016-05-18 | 1876-7737 | Journal of proteomics | Characterizations and proteome analysis of platelet-free plasma-derived microparticles in β-thalassemia/hemoglobin E patients. | Chaichompoo Porntip, Kumya Panida, Khowawisetsut Ladawan, Chiangjong Wararat, Chaiyarit Sakdithep, Pongsakul Nutkridta, Sirithanaratanakul Noppadol, Fucharoen Suthat, Thongboonkerd Visith, Pattanapanyasat Kovit | eng | null | Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Blood Proteins, Proteome, Hemoglobin E | IM | 22705320, S1874-3919(12)00428-9, 10.1016/j.jprot.2012.06.004 | Aggregatability and oxidative damage of red blood cells (RBCs), platelet activation and increased amount of blood cells-derived microparticles (MPs) are thought to be the etiologies for the thrombotic risk in thalassemia, but with unclear mechanisms. Here we report cellular origins and increases in number, oxidative stress status, and procoagulant activity, as well as altered proteome of MPs isolated from β-thal/HbE patients. Flow cytometric analysis revealed that β-thal/HbE patients had significantly higher levels of phosphatidylserine (PS)-bearing MPs in platelet-free plasma (PFP) as compared to normal subjects. The high levels of MPs correlated with not only the increased procoagulant activity but also the increased platelet counts. Additionally, these PS-bearing MPs were originated mostly from platelets and RBCs, both of which had increased levels of reactive oxygen species. Proteome analysis of MPs by 2-DE followed by Q-TOF MS and MS/MS analyses identified 29 proteins with significantly altered levels in MPs derived from β-thal/HbE patients (e.g. the increased levels of peroxiredoxin 6, apolipoprotein E, cyclophilin A and heat shock protein 90). These findings suggest that the oxidative damage in platelets and RBCs potentially induces production of MPs with altered proteome that may, in turn, facilitate thromboembolic complications, which are commonly found in thalassemic patients. This article is part of a Special Issue entitled: Integrated omics. | Adult, Blood Proteins, Cell-Derived Microparticles, Female, Hemoglobin E, Hemoglobinuria, Humans, Male, Plasma, Proteome, Proteomics, Thromboembolism, beta-Thalassemia | null |
22,705,322 | 2013-05-24 | 2020-07-29 | 1873-4847 | The Journal of nutritional biochemistry | Phosphorylated S6K1 (Thr389) is a molecular adipose tissue marker of altered glucose tolerance. | Moreno-Navarrete José María, Ortega Francisco, Sánchez-Garrido Miguel Ángel, Sabater Mònica, Ricart Wifredo, Zorzano Antonio, Tena-Sempere Manuel, Fernández-Real José Manuel | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biomarkers, IRS1 protein, human, Insulin Receptor Substrate Proteins, Thiazolidinediones, Rosiglitazone, Threonine, Ribosomal Protein S6 Kinases, Ribosomal Protein S6 Kinases, 70-kDa, Rps6kb1 protein, rat, ribosomal protein S6 kinase, 70kD, polypeptide 1 | IM | 22705322, S0955-2863(12)00036-8, 10.1016/j.jnutbio.2012.01.005 | Molecular tissue markers of altered glucose metabolism will be useful as potential targets for antidiabetic drugs. S6K1 is a downstream signal of insulin action. We aimed to evaluate (pThr389)S6K1 and total S6K1 levels in human and rat fat depots as candidate markers of altered glucose metabolism. (pThr389)S6K1 and total S6K1 levels were measured using enzyme linked immune sorbent assay (ELISA) in 49 adipose tissue samples from subjects with morbid obesity and in 18 peri-renal white adipose tissue samples from rats. The effects of high glucose and rosiglitazone have been explored in human preadipocytes. (pThr389)S6K1/(total)S6K1 in subcutaneous adipose tissue was significantly increased subjects with Type 2 diabetes (0.78 ± 0.26 vs. 0.55 ± 0.14, P=.02) and associated with fasting glucose (r=0.46, P=.04) and glycated hemoglobin (r=0.63, P=.02) in SAT. Similar associations with fasting glucose (r=0.43, P=.03) and IRS1 (r=-0.41, P=.04) gene expression were found in visceral adipose tissue. In addition, rat experiments confirmed the higher (pThr389)S6K1/totalS6K1 levels in adipose tissue in association with obesity-associated metabolic disturbances. (pThr389)S6K1/totalS6K1 was validated using western blot in rat adipose tissue. Both ELISA and western blot data significantly correlated (r=0.85, P=.005). In human preadipocytes, high glucose medium led to increased (pThr389)S6K1/total S6K1 levels in comparison with normal glucose medium, which was significantly decreased under rosiglitazone administration. In conclusion, in human and rat adipose tissue, phosphorylated S6K1 is a marker for increased glucose levels. | Adipocytes, Adipose Tissue, Adipose Tissue, White, Animals, Biomarkers, Blotting, Western, Diabetes Mellitus, Type 2, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression, Glucose Tolerance Test, Humans, Insulin Receptor Substrate Proteins, Male, Obesity, Morbid, Phosphorylation, Rats, Rats, Wistar, Reference Values, Reproducibility of Results, Ribosomal Protein S6 Kinases, Ribosomal Protein S6 Kinases, 70-kDa, Rosiglitazone, Thiazolidinediones, Threonine | null |
22,705,324 | 2013-11-14 | 2012-09-24 | 1885-5857 | Revista espanola de cardiologia (English ed.) | Electrocardiographic changes underlying central nervous system damage. | López-Lluva María T, Arizón-Muñoz José M, Gonzalez-Ruiz de la Herran Francisco, Marina-Breysse Manuel | eng | null | Case Reports, Letter | null | IM | 22705324, S0300-8932(12)00143-1, 10.1016/j.recesp.2012.02.007 | null | Brain Neoplasms, Central Nervous System Diseases, Coronary Angiography, Electrocardiography, Female, Humans, Lung Neoplasms, Middle Aged, Small Cell Lung Carcinoma, Tomography, X-Ray Computed | null |
22,705,323 | 2013-05-24 | 2021-10-21 | 1873-4847 | The Journal of nutritional biochemistry | Differential effects of dietary sodium intake on blood pressure and atherosclerosis in hypercholesterolemic mice. | Lu Hong, Wu Congqing, Howatt Deborah A, Balakrishnan Anju, Charnigo Richard J, Cassis Lisa A, Daugherty Alan | eng | R01 HL062846 (NHLBI NIH HHS, United States); R01 HL062846-08 (NHLBI NIH HHS, United States); HL062846 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Receptors, LDL, Sodium, Dietary, Aldosterone, Cholesterol, Sodium, Renin | IM | 22705323, S0955-2863(12)00043-5, 10.1016/j.jnutbio.2012.03.001, PMC3568659, NIHMS363732, 15596561, 15893934, 12357958, 17213477, 11157699, 17224157, 15788708, 19085043, 11500177, 18158357, 15277329, 18274671, 21868698, 12105166, 1706034, 9519949, 18957695, 16403692, 19488026, 22153700, 18262533, 18287665, 3416162, 15023892, 10842656, 15910852, 12562870, 19037590, 21540421, 15585195, 17196676, 18045096, 16157344, 12372775, 18645051, 17724261, 19145766, 15878965, 10841519 | The amount of dietary sodium intake regulates the renin angiotensin system (RAS) and blood pressure, both of which play critical roles in atherosclerosis. However, there are conflicting findings regarding the effects of dietary sodium intake on atherosclerosis. This study applied a broad range of dietary sodium concentrations to determine the concomitant effects of dietary sodium intake on the RAS, blood pressure, and atherosclerosis in mice. Eight-week-old male low-density lipoprotein receptor -/- mice were fed a saturated fat-enriched diet containing selected sodium concentrations (Na 0.01%, 0.1%, or 2% w/w) for 12 weeks. Mice in these three groups were all hypercholesterolemic, although mice fed Na 0.01% and Na 0.1% had higher plasma cholesterol concentrations than mice fed Na 2%. Mice fed Na 0.01% had greater abundances of renal renin mRNA than those fed Na 0.1% and 2%. Plasma renin concentrations were higher in mice fed Na 0.01% (14.2 ± 1.7 ng/ml/30 min) than those fed Na 0.1% or 2% (6.2 ± 0.6 and 5.8 ± 1.6 ng/ml per 30 min, respectively). However, systolic blood pressure at 12 weeks was higher in mice fed Na 2% (138 ± 3 mm Hg) than those fed Na 0.01% and 0.1% (129 ± 3 and 128 ± 4 mmHg, respectively). In contrast, mice fed Na 0.01% (0.17 ± 0.02 mm(2)) had larger atherosclerotic lesion areas in aortic roots than those fed Na 2% (0.09 ± 0.01 mm(2)), whereas lesion areas in mice fed Na 0.1% (0.12 ± 0.02 mm(2)) were intermediate between and not significantly different from those in Na 0.01% and Na 2% groups. In conclusion, while high dietary sodium intake led to higher systolic blood pressure, low dietary sodium intake augmented atherosclerosis in hypercholesterolemic mice. | Aldosterone, Animals, Aorta, Atherosclerosis, Blood Pressure, Cholesterol, Diet, High-Fat, Dose-Response Relationship, Drug, Hypercholesterolemia, Male, Mice, Mice, Mutant Strains, Receptors, LDL, Renin, Sodium, Sodium, Dietary | null |
22,705,326 | 2013-01-15 | 2023-12-13 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Regular black tea habit could reduce tobacco associated ROS generation and DNA damage in oral mucosa of normal population. | Pal Debolina, Sur Subhayan, Mandal Shyamsundar, Das Sukta, Panda Chinmay Kumar | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Reactive Oxygen Species, Tea | IM | 22705326, S0278-6915(12)00419-X, 10.1016/j.fct.2012.06.005 | Tobacco and tea habit are very common in world wide. In the present study, an attempt was made to evaluate the effect of regular drinking of black tea on reactive oxygen species (ROS) generation and DNA damage in buccal cells of normal subjects with or without tobacco habit. Expression of ROS associated proteins IκB, NF-κB as well as DNA repair associated proteins p53, MLH1 were also analyzed. Exfoliated buccal cells were collected from 308 healthy individuals and classified according to age, tobacco and tea habits. In all age groups, comparatively high ROS level and significantly high DNA damage frequency were seen in individuals with tobacco habit than the subjects without tea and tobacco habits. Tea habit effectively lowered ROS level and restrict DNA damage in tobacco users irrespective of ages. The DNA damage seen in the subjects was not associated with apoptosis. Moreover, tea habit effectively lowered the expression of IκB, NF-κB, p53 and MLH1 in tobacco users in all age groups. It seems that regular black tea habit could have anti-genotoxic effect as revealed by reduced tobacco associated ROS generation and DNA damage in buccal cells. | Aged, Comet Assay, DNA Damage, Female, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Male, Middle Aged, Mouth Mucosa, Reactive Oxygen Species, Tea, Nicotiana | null |
22,705,325 | 2013-01-15 | 2012-08-20 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Anti-skin cancer properties of phenolic-rich extract from the pericarp of mangosteen (Garcinia mangostana Linn.). | Wang Jing J, Shi Qing H, Zhang Wei, Sanderson Barbara J S | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anticarcinogenic Agents, DNA Primers, Phenols, Plant Extracts, Caspases | IM | 22705325, S0278-6915(12)00417-6, 10.1016/j.fct.2012.06.003 | Skin cancers are often resistant to conventional chemotherapy. This study examined the anti-skin cancer properties of crude ethanol extract of mangosteen pericarp (MPEE) on human squamous cell carcinoma A-431 and melanoma SK-MEL-28 lines. Significant dose-dependent reduction in% viability was observed for these cell lines, with less effect on human normal skin fibroblast CCD-1064Sk and keratinocyte HaCaT cell lines. Cell distribution in G(1) phase (93%) significantly increased after 10 μg/ml of MPEE versus untreated SK-MEL-28 cells (78%), which was associated with enhanced p21(WAF1) mRNA levels. In A-431 cells, 10 μg/ml MPEE significantly increased the sub G(1) peak (15%) with concomitant decrease in G(1) phase over untreated cells (2%). In A-431 cells, 10 μg/ml MPEE induced an 18% increase in early apoptosis versus untreated cells (2%). This was via caspase activation (15-, 3- and 4-fold increased caspse-3/7, 8, and 9 activities), and disruption of mitochondrial pathways (6-fold decreased mitochondrial membrane potential versus untreated cells). Real-time PCR revealed increased Bax/Bcl-2 ratio and cytochrome c release, and decreased Akt1. Apoptosis was significantly increased after MPEE treatment of SK-MEL-28 cells. Hence, MPEE showed strong anti-skin cancer effect on these two skin cancer cell lines, with potential as an anti-skin cancer agent. | Anticarcinogenic Agents, Apoptosis, Base Sequence, Caspases, Cell Cycle, Cell Line, Tumor, DNA Primers, Enzyme Activation, Garcinia mangostana, Humans, Phenols, Plant Extracts, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Skin Neoplasms | null |
22,705,328 | 2013-01-15 | 2012-08-20 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | 6-(Propan-2-yl)-3-methyl-morpholine-2,5-dione, a novel cyclodidepsipeptide with modulatory effect on rat thymocytes. | Pavlovic Voja, Cherneva Emiliya, Yancheva Denitsa, Smelcerovic Andrija | eng | null | Journal Article, Research Support, Non-U.S. Gov't | 6-(propan-2-yl)-3-methyl-morpholine-2,5-dione, Culture Media, Morpholines, Reactive Oxygen Species | IM | 22705328, S0278-6915(12)00418-8, 10.1016/j.fct.2012.06.004 | A study has been carried out on the potential effect of a novel cyclodidepsipeptide, 6-(propan-2-yl)-3-methyl-morpholine-2,5-dione (PMMD), on rat thymocytes. Rat thymocytes were cultivated with increasing PMMD concentrations (0.1, 1, 10 μg/well), for 24h, and evaluated for proliferative activity, viability, reactive oxygen species and mitochondrial membrane potential. The higher PMMD concentrations inhibited thymocytes proliferative activity mainly through induction of oxidative stress and resulting cytotoxicity, without any mitochondrial membrane potential alterations in thymocytes. The obtained results are correlated with previously published data on effects of 6-(propan-2-yl)-4-methyl-morpholine-2,5-diones on rat thymocytes. The presence of methyl group in position 4 or/and the length of alkyl chain in position 3 of 6-(propan-2-yl)-morpholine-2,5-dione core plays a role for the obtained differences in the biological response between PMMD and two previously tested 6-(propan-2-yl)-4-methyl-morpholine-2,5-diones. | Animals, Cell Proliferation, Cells, Cultured, Culture Media, Male, Membrane Potential, Mitochondrial, Morpholines, Rats, Rats, Wistar, Reactive Oxygen Species, Thymus Gland | null |
22,705,327 | 2013-01-15 | 2021-12-03 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Protective effect of allicin against acrylamide-induced hepatocyte damage in vitro and in vivo. | Zhang Lulu, Zhang Huanjie, Miao Yutian, Wu Sijia, Ye Haiqing, Yuan Yuan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Disulfides, Sulfinic Acids, Acrylamide, allicin, Malondialdehyde, 8-Hydroxy-2'-Deoxyguanosine, Deoxyguanosine | IM | 22705327, S0278-6915(12)00413-9, 10.1016/j.fct.2012.05.060 | Acrylamide (AA) is known to be a neurotoxic, genotoxic, and carcinogenic compound. The presence of AA in foods causes public health concerns. In our previous study, we found that allicin can effectively reduce AA content in Maillard model system. However, there has been no report on whether allicin can reduce AA-induced toxicity in vitro and in vivo. In our present study, we evaluated the protective effect of allicin against AA-induced hepatocyte damage in cultured mouse primary hepatocytes and mouse liver. Our date suggested that allicin significantly decreased the levels of maleic dialdehyde (MDA) and 8-hydroxy-desoxyguanosine (8-OHdG) both in vitro and in vivo study. Allicin also markedly increased the activity of total superoxide dismutase (SOD) and level of glutathione (GSH). The in vitro study revealed that 15 μM allicin was the optimum concentration for inhibiting AA-induced hepatocyte damage. The in vivo study revealed that 20mg/kg b.w./day allicin was the optimum dose for inhibiting AA-induced hepatocyte damage. The protective effects of allicin against AA-induced hepatocyte damage may be due to its ability to scavenge free radicals and its effective recovery of the antioxidative defense system, and its ability to block the epoxidation process of AA to GA by inhibiting P450 enzyme. | 8-Hydroxy-2'-Deoxyguanosine, Acrylamide, Animals, Body Weight, Cells, Cultured, Deoxyguanosine, Disulfides, Hepatocytes, In Vitro Techniques, Maillard Reaction, Male, Malondialdehyde, Mice, Mice, Inbred BALB C, Organ Size, Sulfinic Acids | null |
22,705,329 | 2013-01-15 | 2016-11-25 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Mulberry water extracts (MWEs) ameliorated carbon tetrachloride-induced liver damages in rat. | Hsu Li-Sung, Ho Hsieh-Hsun, Lin Ming-Cheng, Chyau Charng-Cherng, Peng Jih-Shin, Wang Chau-Jong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Plant Extracts, Water, Carbon Tetrachloride, Aspartate Aminotransferases, Alanine Transaminase, Alkaline Phosphatase | IM | 22705329, S0278-6915(12)00408-5, 10.1016/j.fct.2012.05.055 | Mulberry extracts are antidiabetic and antihyperlipidemic, as well as preventive of cardiovascular disease. The current study investigates the protective mechanisms of mulberry water extracts (MWEs) in carbon tetrachloride (CCl(4))-induced hepatic injury. Oral administration of MWEs significantly reduced the lipid peroxidation triggered by CCl(4), as shown by the reduced production of thiobarituric acid-reactive substance (TBARS). The levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were also reduced via cotreatment with MWEs compared with CCl(4) treatment alone. Cotreatment with MWE evidently reduced CCl(4)-induced liver weight and inhibited lipid deposition and fibrogenesis. In a similar manner, cotreatment with silymarin, a well-known liver protective agent, also reversed the CCl(4)-induced effects, such as reduced TBARS formation, decreased serum AST, ALT, and ALP levels, blocked lipid accumulation, and liver fibrosis. Furthermore, MWEs attenuated the proinflammatory genes such as cyclooxygenase 2, nuclear factor kappa B, and inducible nitric oxide synthase expression. The current findings suggest that MWEs such as silymarin exhibit protective and curative effects against CCl(4)-induced liver damage and fibrosis via decreased lipid peroxidation and inhibited proinflammatory gene expression. | Alanine Transaminase, Alkaline Phosphatase, Animals, Aspartate Aminotransferases, Carbon Tetrachloride, Chemical and Drug Induced Liver Injury, Lipid Peroxidation, Male, Morus, Plant Extracts, Rats, Rats, Wistar, Water | null |
22,705,330 | 2013-01-15 | 2014-11-20 | 1873-6351 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Angelicae Gigantis Radix regulates mast cell-mediated allergic inflammation in vivo and in vitro. | Sohn Youngjoo, Lee Hyun-Sam, Park Hi-Joon, Lee Hyangsook, Lee Hyejung, Choi Hyeon, Jeong Chang-Hyun, Bu Youngmin, Jung Hyuk-Sang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Plant Extracts | IM | 22705330, S0278-6915(12)00414-0, 10.1016/j.fct.2012.06.001 | Angelicae Gigantis (AG) Radix, commonly used medicinal food, has been reported as a promising candidate for inflammatory diseases. However, the anti-allergic effects of AG and its molecular mechanisms have yet to be clarified. The present study investigated the anti-allergy effects of ethanol extracts of AG on mast cell-mediated allergic inflammation in vivo and in vitro. The finding of this study demonstrated that AG reduced anti-dinitrophenyl IgE antibody-induced passive cutaneous anaphylaxis, compound 48/80-induced histamine release, 2,4-dinitrofluoro benzene-induced contact hypersensitivity. In addition, AG inhibited the production of interleukin (IL)-6, IL-8, and TNF-α, as well as the activation of Jun N-terminal kinase and nuclear factor-κB in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated human mast cells. In conclusion, our results provide a novel insight into the pharmacological actions of AG as a potential candidate for use in allergic inflammatory diseases. | Angelica, Animals, Chromatography, High Pressure Liquid, Female, Histamine Release, Hypersensitivity, In Vitro Techniques, Inflammation, Mast Cells, Mice, Mice, Inbred BALB C, Plant Extracts, Rats, Rats, Sprague-Dawley | null |
22,705,331 | 2013-01-24 | 2016-11-25 | 1879-3177 | Toxicology in vitro : an international journal published in association with BIBRA | Toxicity evaluation of CdTe quantum dots with different size on Escherichia coli. | Fang Ting-Ting, Li Xi, Wang Qi-Sui, Zhang Zhi-Jun, Liu Peng, Zhang Chao-Can | eng | null | Journal Article | Cadmium Compounds, Tellurium, cadmium telluride | IM | 22705331, S0887-2333(12)00151-8, 10.1016/j.tiv.2012.06.001 | Quantum dots (QDs) have a great potential for applications in nanomedicine. However, a few studies showed that they also exhibited toxicity. We used Escherichia coli (E. coli) as the model to study the effect of CdTe QDs on the cell growth by microcalorimetric technique, optical density (OD(600)) and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Three size aqueous-compatible CdTe QDs with maximum emission of 543 nm (green-emitting QDs, GQDs), 579 nm (yellow-emitting QDs, YQDs) and 647 nm (red-emitting QDs, RQDs) were tested. The growth rate constants (k) and half-inhibiting concentration (IC(50)) were calculated from the microcalorimetric data. The results indicated that CdTe QDs exhibited a dose-dependent inhibitory effect on cell growth. The order of toxicity is GQDs>YQDs>RQDs. The smaller the particle size of QDs is, the more toxicity it is. ATR-FTIR spectra indicated that the outer membrane of the cell was changed or damaged by the QDs, which may induce QDs and harmful by-products to enter into the cells. These could be one of the reasons that CdTe QDs have cytotoxic effects on E. coli. | Animal Testing Alternatives, Cadmium Compounds, Escherichia coli, Inhibitory Concentration 50, Particle Size, Quantum Dots, Tellurium, Toxicity Tests | null |
22,705,334 | 2013-03-22 | 2013-11-21 | 1950-6007 | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Conversion of estrone to 17-beta-estradiol in human non-small-cell lung cancer cells in vitro. | Drzewiecka Hanna, Jagodzinski Paweł P | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Estrone, Estradiol, 17-Hydroxysteroid Dehydrogenases, 3 (or 17)-beta-hydroxysteroid dehydrogenase | IM | 22705334, S0753-3322(12)00039-X, 10.1016/j.biopha.2012.02.006 | It has recently been suggested that, in addition to genetic and environmental factors and tobacco exposure, estrogens also may be an independent risk factor in the development of lung cancer. Therefore, we evaluated the transcript and protein levels of 17-beta-hydroxysteroid-dehydrogenase type 1 (HSD17B1), and the conversion of estrone (E1) to 17-beta-estradiol (E2) in human non-small cell lung cancer (NSCLC) Calu-6, Calu-1 and A549 cells. In our work, we established the presence of HSD17B1 transcripts and proteins in all examined NSCLC cells. Moreover, we demonstrated that human NSCLC Calu-6, Calu-1 and A549 cells are able to convert weak estrogen E1 to highly potent E2 in vitro. Our results indicate that NSCLC cells are able to produce E2 from E1. | 17-Hydroxysteroid Dehydrogenases, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Estradiol, Estrone, Humans, Lung Neoplasms, Risk Factors | null |
22,705,332 | 2012-10-09 | 2012-07-23 | 1878-5646 | Vision research | Do serifs help in comprehension of printed text? An experiment with Cyrillic readers. | Akhmadeeva Leyla, Tukhvatullin Ilnar, Veytsman Boris | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705332, S0042-6989(12)00172-1, 10.1016/j.visres.2012.05.013 | The role of serifs for the comprehension of printed text has been controversial in the literature. The analysis was often confounded by the fact that fonts used for comparison have many differences besides being serif or sans-serif. In this study we use fonts from the same foundry and meta family to assess the differences in reading comprehension for Cyrillic readers (n=238). The results show no difference in the speed of reading and the comprehension between the serif and sans-serif texts. This conclusion is consistent with the ecological hypothesis recently formulated for font sizes. | Comprehension, Form Perception, Humans, Language, Photic Stimulation, Psychophysics, Reading, Time Factors | null |
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