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stringclasses 7
values | technique
stringclasses 3
values | relationship/description/definition/use
stringlengths 12
87
| factor/label/component/method/mechanism
stringlengths 3
45
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Migration rate dependency
|
Electrophoresis
|
Directly proportional to rate of movement
|
Charge of the molecule
|
Migration rate dependency
|
Electrophoresis
|
Inversely proportional to rate of movement
|
Size of the molecule
|
Migration rate dependency
|
Electrophoresis
|
Increased current increases migration rate
|
Electrical field
|
Migration rate dependency
|
Electrophoresis
|
Increased ionic strength decreases migration rate
|
Ionic strength of buffer
|
Migration rate dependency
|
Electrophoresis
|
Decreased pH slows migration
|
pH of buffer
|
Migration rate dependency
|
Electrophoresis
|
Inversely proportional to migration rate
|
Viscosity of supporting medium
|
Migration rate dependency
|
Electrophoresis
|
High temperature can denature protein and slow migration
|
System temperature
|
Analytic electrophoretic procedures
|
Electrophoresis
|
Proteins are amphoteric...
|
Protein electrophoresis
|
Analytic electrophoretic procedures
|
Electrophoresis
|
Similar to protein electrophoresis...
|
Isoenzyme electrophoresis
|
Components
|
Immunoassay
|
A substance that can elicit an immune response...
|
Antigen
|
Components
|
Immunoassay
|
An immunoglobulin formed in response to a foreign substance...
|
Antibody
|
Immunochemical labels
|
Immunoassay
|
Attached to the antibody, allowing colorimetric quantitation...
|
Enzyme labels
|
Immunochemical labels
|
Immunoassay
|
Emit photons when excited, used in fluorescence-based detection...
|
Fluorescent labels
|
Immunochemical labels
|
Immunoassay
|
Release energy in the form of visible light upon returning to ground state...
|
Chemiluminescent labels
|
Immunochemical labels
|
Immunoassay
|
Emit radiation (gamma rays) upon decay, detected for quantitation...
|
Radioisotope labels
|
Methodologies
|
Immunoassay
|
Enzyme-based
|
ELISA
|
Methodologies
|
Immunoassay
|
Enzyme-based
|
EIA/EMIT
|
Methodologies
|
Immunoassay
|
Fluorescence-based
|
FPIA
|
Methodologies
|
Immunoassay
|
Fluorescence-based
|
FIA
|
Methodologies
|
Immunoassay
|
Radiation-based
|
RIA
|
Methodologies
|
Immunoassay
|
Radiation-based
|
IRMA
|
Mechanisms of separation
|
Chromatography
|
Based on the competition between the sample and the mobile phase for binding sites...
|
Adsorption chromatography
|
Mechanisms of separation
|
Chromatography
|
Depends on the solubility of the solute in nonpolar or polar solvents...
|
Partition chromatography
|
Mechanisms of separation
|
Chromatography
|
Involves separation of solutes by their size and charge of the ionic species present...
|
Ion-exchange chromatography
|
Chromatographic procedures
|
Chromatography
|
Semi-quantitative screening test...
|
Thin-layer chromatography (TLC)
|
Chromatographic procedures
|
Chromatography
|
Provides quantitative results...
|
High-performance liquid chromatography (HPLC)
|
Migration rate dependency
|
Electrophoresis
|
Directly proportional to rate of movement
|
Charge of the molecule
|
Migration rate dependency
|
Electrophoresis
|
Inversely proportional to rate of movement
|
Size of the molecule
|
Migration rate dependency
|
Electrophoresis
|
Increased current increases migration rate
|
Electrical field
|
Migration rate dependency
|
Electrophoresis
|
Increased ionic strength decreases migration rate
|
Ionic strength of buffer
|
Migration rate dependency
|
Electrophoresis
|
Decreased pH slows migration
|
pH of buffer
|
Migration rate dependency
|
Electrophoresis
|
Inversely proportional to migration rate
|
Viscosity of supporting medium
|
Migration rate dependency
|
Electrophoresis
|
High temperature can denature protein and slow migration
|
System temperature
|
Analytic electrophoretic procedures
|
Electrophoresis
|
Proteins are amphoteric...
|
Protein electrophoresis
|
Analytic electrophoretic procedures
|
Electrophoresis
|
Similar to protein electrophoresis...
|
Isoenzyme electrophoresis
|
Components
|
Immunoassay
|
A substance that can elicit an immune response...
|
Antigen
|
Components
|
Immunoassay
|
An immunoglobulin formed in response to a foreign substance...
|
Antibody
|
Immunochemical labels
|
Immunoassay
|
Attached to the antibody, allowing colorimetric quantitation...
|
Enzyme labels
|
Immunochemical labels
|
Immunoassay
|
Emit photons when excited, used in fluorescence-based detection...
|
Fluorescent labels
|
Immunochemical labels
|
Immunoassay
|
Release energy in the form of visible light upon returning to ground state...
|
Chemiluminescent labels
|
Immunochemical labels
|
Immunoassay
|
Emit radiation (gamma rays) upon decay, detected for quantitation...
|
Radioisotope labels
|
Methodologies
|
Immunoassay
|
Enzyme-based
|
ELISA
|
Methodologies
|
Immunoassay
|
Enzyme-based
|
EIA/EMIT
|
Methodologies
|
Immunoassay
|
Fluorescence-based
|
FPIA
|
Methodologies
|
Immunoassay
|
Fluorescence-based
|
FIA
|
Methodologies
|
Immunoassay
|
Radiation-based
|
RIA
|
Methodologies
|
Immunoassay
|
Radiation-based
|
IRMA
|
Mechanisms of separation
|
Chromatography
|
Based on the competition between the sample and the mobile phase for binding sites...
|
Adsorption chromatography
|
Mechanisms of separation
|
Chromatography
|
Depends on the solubility of the solute in nonpolar or polar solvents...
|
Partition chromatography
|
Mechanisms of separation
|
Chromatography
|
Involves separation of solutes by their size and charge of the ionic species present...
|
Ion-exchange chromatography
|
Chromatographic procedures
|
Chromatography
|
Semi-quantitative screening test...
|
Thin-layer chromatography (TLC)
|
Chromatographic procedures
|
Chromatography
|
Provides quantitative results...
|
High-performance liquid chromatography (HPLC)
|
This is a dataset for training AI on medical tools and practices in the modern age.
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