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22,706,178 | 2013-11-14 | 2022-03-10 | 1537-453X | American journal of clinical oncology | Impact of adjuvant radiation therapy for microscopic residual tumor after resection of extrahepatic bile duct cancer. | Matsuda Takeru, Fujita Hirofumi, Harada Naoki, Kunimoto Yukihiro, Tanaka Tomohiro, Kimura Taisei, Kitaoka Hironori, Asano Eisuke, Hosono Masayoshi, Hayashi Tomomi, Ogino Kazunori | eng | null | Journal Article | null | IM | 22706178, 10.1097/COC.0b013e31825494ab | The effect of adjuvant radiation therapy (RT) in extrahepatic bile duct (EHBD) cancer patients with microscopic-positive resection margins (R1 resection) is still controversial. | Adult, Aged, Aged, 80 and over, Bile Duct Neoplasms, Bile Ducts, Extrahepatic, Combined Modality Therapy, Female, Follow-Up Studies, Humans, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Neoplasm, Residual, Prognosis, Radiotherapy, Adjuvant, Retrospective Studies, Survival Rate | null |
22,706,175 | 2013-11-14 | 2022-12-07 | 1537-453X | American journal of clinical oncology | Phase II trial of pemetrexed plus gemcitabine in patients with locally advanced and metastatic nonclear cell renal cell carcinoma. | Richey Stephen L, Tamboli Pheroze, Ng Chaan S, Lin E, Lim Zita D, Araujo John C, Jonasch Eric, Sharma Padmanee, Pagliaro Lance C, Tannir Nizar M | eng | P30 CA016672 (NCI NIH HHS, United States) | Clinical Trial, Phase II, Journal Article, Research Support, Non-U.S. Gov't | Glutamates, Pemetrexed, Deoxycytidine, Guanine, Gemcitabine | IM | 22706175, 10.1097/COC.0b013e3182546a91, PMC3882166, NIHMS535617, 8518225, 15505625, 20711632, 11981011, 20421819, 19884559, 18506025, 19767093, 19487915, 15117980, 19487381, 8599874, 11890331, 18156031, 21172884, 12860938, 11106124, 16873433, 18165647, 16087696, 18635226, 17215529, 20299319, 14578684, 21355978, 10655437, 10647643, 18653228, 8960143, 16909426, 17538086, 10856102, 17215530 | To determine the clinical activity and safety of the combination of pemetrexed and gemcitabine in advanced nonclear cell renal cell carcinoma (nccRCC). | Adolescent, Adrenal Gland Neoplasms, Adult, Aged, Antineoplastic Combined Chemotherapy Protocols, Bone Neoplasms, Carcinoma, Renal Cell, Deoxycytidine, Female, Follow-Up Studies, Glutamates, Guanine, Humans, Infusions, Intravenous, Kidney Neoplasms, Liver Neoplasms, Lung Neoplasms, Male, Middle Aged, Neoplasm Grading, Neoplasm Recurrence, Local, Neoplasm Staging, Pemetrexed, Prognosis, Survival Rate, Young Adult, Gemcitabine | null |
22,706,174 | 2013-11-14 | 2022-04-10 | 1537-453X | American journal of clinical oncology | Delayed systemic recurrence of uveal melanoma. | Kolandjian Nathalie A, Wei Caimiao, Patel Sapna P, Richard Jessica L, Dett Tina, Papadopoulos Nicholas E, Bedikian Agop Y | eng | P30 CA016672 (NCI NIH HHS, United States); CA016672 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22706174, 10.1097/COC.0b013e3182546a6b, PMC4574291, NIHMS369182, 2917216, 11346394, 2375648, 16365561, 15197206, 17050279, 17957189, 7848381, 7244714, 20070457, 6678974, 6860127, 8635073, 16303922, 20164465, 16344433, 17159027, 9620065, 10892838, 4038058, 8280680, 19078957, 20061986, 11773005, 3734186, 12436444, 2023760, 14578381, 18719078, 16258106, 14692032 | Metastatic uveal melanoma recurrence after ≥10 years is not well studied in the clinical literature. This study describes the clinical characteristics and natural history of patients with delayed tumor recurrence. | Adolescent, Adult, Aged, Aged, 80 and over, Clinical Trials as Topic, Combined Modality Therapy, Female, Follow-Up Studies, Humans, Liver Neoplasms, Melanoma, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Prognosis, Survival Rate, Time Factors, Uveal Neoplasms, Young Adult | null |
22,706,180 | 2013-11-14 | 2022-03-11 | 1537-453X | American journal of clinical oncology | Survival and intracranial control of patients with 5 or more brain metastases treated with gamma knife stereotactic radiosurgery. | Raldow Ann C, Chiang Veronica L, Knisely Jonathan P, Yu James B | eng | null | Journal Article | null | IM | 22706180, 10.1097/COC.0b013e31825494ef | Limited data are available to help inform decisions about stereotactic radiosurgery for patients with ≥5 brain metastases. We therefore performed a retrospective analysis of patients treated for >5 brain metastases. | Adult, Aged, Brain Neoplasms, Female, Follow-Up Studies, Humans, Male, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Neoplasms, Prognosis, Radiosurgery, Retrospective Studies, Survival Rate | null |
22,706,179 | 2013-11-14 | 2013-09-23 | 1537-453X | American journal of clinical oncology | Treatment of mycosis fungoides with total skin electron beam: response and relapse by ethnicity and sex. | Hinds Ginette A, Alhariri Jihad, Klein Rhonda Q, Wilson Lynn D | eng | null | Journal Article | null | IM | 22706179, 10.1097/COC.0b013e31825494d3 | To determine whether clinical response to total skin electron beam (TSEB) and relapse after TSEB differs by ethnicity and sex. | Aged, Electrons, Female, Follow-Up Studies, Humans, Male, Middle Aged, Mycosis Fungoides, Neoplasm Recurrence, Local, Prognosis, Retrospective Studies, Sex Factors, Skin Neoplasms, Survival Rate, Whole-Body Irradiation | null |
22,706,177 | 2013-11-14 | 2013-09-23 | 1537-453X | American journal of clinical oncology | Postsurgical treatment of early-stage breast cancer with electronic brachytherapy: outcomes and health-related quality of life at 1 year. | Patel Rakesh R, Beitsch Peter D, Nichols Timothy D, Lorenzetti John D, Wurzer James C, Tucker James C, Nunn George W, Laduzinsky Susan J, Kugler Morris A, Simmons Dwelvin L, Gilligan Michael S, Roy Tapan, Foley Jonathon K, Thropay John P, Odou Mark William, Bornstein Bruce A, Tito Elizabeth P, Chadha Manjeet, Boolbol Susan K, Lane Steven C, White Julie G | eng | null | Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't | null | IM | 22706177, 10.1097/COC.0b013e31825493bb | This multicenter registry followed up patients with early-stage breast cancer treated with breast-conserving surgery and electronic brachytherapy (EBT). This report provides 1- and 2-year updates to the initial publication. | Adult, Aged, Aged, 80 and over, Brachytherapy, Breast Neoplasms, Carcinoma, Ductal, Breast, Carcinoma, Intraductal, Noninfiltrating, Female, Follow-Up Studies, Humans, Middle Aged, Neoplasm Grading, Neoplasm Invasiveness, Neoplasm Recurrence, Local, Prognosis, Quality of Life, Registries, Survival Rate | null |
22,706,182 | 2012-11-29 | 2018-12-01 | 1537-6613 | The Journal of infectious diseases | Chikungunya virus transmission--more than meets the eye. | Long Kristin M, Heise Mark T | eng | null | Editorial, Comment | null | IM | 22706182, jis297, 10.1093/infdis/jis297 | null | Alphavirus Infections, Animals, Chikungunya virus, Cornea, Corneal Transplantation, Female, Humans, Male | null |
22,706,181 | 2012-10-16 | 2013-11-21 | 1464-0333 | Journal of environmental monitoring : JEM | Arsenic mobilization and attenuation by mineral-water interactions: implications for managed aquifer recharge. | Neil Chelsea W, Yang Y Jeffrey, Jun Young-Shin | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Water Pollutants, Chemical, Arsenic | IM | 22706181, 10.1039/c2em30323j | Managed aquifer recharge (MAR) has potential for addressing deficits in water supplies worldwide. It is also widely used for preventing saltwater intrusion, maintaining the groundwater table, and augmenting ecological stream flows, among many other beneficial environmental applications. However, field MAR sites have experienced arsenic mobilization from aquifer formation minerals due to induced changes in groundwater chemistry. To address this environmental concern, it is crucial to understand the potential sources and sinks impacting arsenic mobilization. This paper outlines important mineral-water interactions that can occur at MAR sites. Detailed information on minerals of concern, physiochemical processes for arsenic mobilization or attenuation, and the potential impact of microbial activity and hydrology on these processes is provided. Based on these mineral-water interactions, guidelines for predicting arsenic mobility are presented, and recommendations are made concerning MAR site monitoring. The review emphasizes important aspects in correlating interfacial reactions to reactive transport modeling and elucidating future challenges, a first step toward developing safer and more sustainable MAR operations. | Arsenic, Environmental Monitoring, Groundwater, Models, Chemical, Water Pollutants, Chemical | null |
22,706,183 | 2012-11-29 | 2012-09-03 | 1537-6613 | The Journal of infectious diseases | Chikungunya virus infection of corneal grafts. | Couderc Thérèse, Gangneux Nicolas, Chrétien Fabrice, Caro Valérie, Le Luong Tan, Ducloux Bernadette, Tolou Hugues, Lecuit Marc, Grandadam Marc | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antibodies, Viral, Immunoglobulin G, Immunoglobulin M, Interferon Type I | IM | 22706183, jis296, 10.1093/infdis/jis296 | Chikungunya virus (CHIKV) is an arbovirus with a high potential to spread globally. We investigated whether CHIKV is transmittable via corneal grafts. | Adolescent, Adult, Aged, Aged, 80 and over, Alphavirus Infections, Animals, Antibodies, Viral, Chikungunya virus, Child, Child, Preschool, Cornea, Corneal Transplantation, Female, Humans, Immunoglobulin G, Immunoglobulin M, Interferon Type I, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Middle Aged, Reunion, Viremia, Young Adult | null |
22,706,184 | 2012-12-11 | 2014-11-20 | 1879-1026 | The Science of the total environment | Water chemistry and nutrient release during the resuspension of FeS-rich sediments in a eutrophic estuarine system. | Morgan Bree, Rate Andrew W, Burton Edward D | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Sulfides, Water Pollutants, Chemical, Water, Iron | IM | 22706184, S0048-9697(12)00747-4, 10.1016/j.scitotenv.2012.05.065 | The objective of this study was to investigate the impact of resuspending FeS-rich benthic sediment on estuarine water chemistry. To address this objective, we conducted (1) a series of laboratory-based sediment resuspension experiments and (2) also monitored changes in surface water composition during field-based sediment resuspension events that were caused by dredging activities in the Peel-Harvey Estuary, Western Australia. Our laboratory resuspension experiments showed that the resuspension of FeS-rich sediments rapidly deoxygenated estuarine water. In contrast, dredging activities in the field did not noticeably lower O(2) concentrations in adjacent surface water. Additionally, while FeS oxidation in the laboratory resuspensions caused measurable decreases in pH, the field pH was unaffected by the dredging event and dissolved trace metal concentrations remained very low throughout the monitoring period. Dissolved ammonium (NH(4)(+)) and inorganic phosphorus (PO(4)-P) were released into the water column during the resuspension of sediments in both the field and laboratory. Following its initial release, PO(4)-P was rapidly removed from solution in the laboratory-based (<1h) and field-based (<100 m from sediment disposal point) investigations. In comparison to PO(4)-P, NH(4)(+) release was observed to be more prolonged over the 2-week period of the laboratory resuspension experiments. However, our field-based observations revealed that elevated NH(4)(+) concentrations were localised to <100 m from the sediment disposal point. This study demonstrates that alongside the emphasis on acidification, deoxygenation and metal release during FeS resuspension, it is important to consider the possibility of nutrient release from disturbed sediments in eutrophic estuaries. | Environmental Monitoring, Environmental Restoration and Remediation, Estuaries, Geologic Sediments, Iron, Sulfides, Water, Water Movements, Water Pollutants, Chemical, Western Australia | null |
22,706,185 | 2013-06-25 | 2013-11-21 | 1872-9649 | Ageing research reviews | Age-related changes in the response of intestinal cells to 1α,25(OH)2-vitamin D3. | Gonzalez Pardo Verónica, Russo de Boland Ana | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Calcium Channel Agonists, Receptors, Calcitriol, Receptors, Phospholipase A2, Vitamins, Phosphatidylinositol 3-Kinases, src-Family Kinases, Mitogen-Activated Protein Kinases, Protein Tyrosine Phosphatases, Phospholipase D, Calcitriol | IM | 22706185, S1568-1637(12)00086-4, 10.1016/j.arr.2012.06.001 | The hormonally active form of vitamin D(3), 1α,25(OH)(2)-vitamin D(3), acts in intestine, its major target tissue, where its actions are of regulatory and developmental importance: regulation of intracellular calcium through modulation of second messengers and activation of mitogenic cascades leading to cell proliferation. Several causes have been postulated to modify the hormone response in intestinal cells with ageing, among them, alterations of vitamin D receptor (VDR) levels and binding sites, reduced expression of G-proteins and hormone signal transduction changes. The current review summarizes the actual knowledge regarding the molecular and biochemical basis of age-impaired 1α,25(OH)(2)-vitamin D(3) receptor-mediated signaling in intestinal cells. A fundamental understanding why the hormone functions are impaired with age will enhance our knowledge of its importance in intestinal cell physiology. | Aging, Animals, Calcitriol, Calcium Channel Agonists, Calcium Signaling, Cell Differentiation, Cell Proliferation, Enterocytes, Humans, Intestines, Mitogen-Activated Protein Kinases, Phosphatidylinositol 3-Kinases, Phospholipase D, Protein Tyrosine Phosphatases, Receptors, Calcitriol, Receptors, Phospholipase A2, Signal Transduction, Vitamins, src-Family Kinases | null |
22,706,187 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development. | Banner David, Kelvin Alyson A | eng | null | Journal Article, Review | Influenza Vaccines | IM | 22706187, 10.3855/jidc.2666 | Highly pathogenic avian influenza H5N1 is a threat to global public health as a natural pandemic causing agent but has recently been considered a bioterrorism concern. The evolving view of the H5N1 virus necessitates the re-evaluation of the current status of H5N1 therapeutics and prophylactics, in particular the preparation of viable H5N1 vaccination strategies as well as the use of ferrets in influenza research. Here the highly pathogenic H5N1 virus dilemma is discussed in context with the current H5N1 vaccine status and the use of the ferret model. Previously, the development of various H5N1 vaccine platforms have been attempted, many of them tested in the ferret model, including vector vaccines, adjuvant vaccines, DNA vaccines, and reverse engineered vaccines. Moreover, as ferrets are a superlative animal model for influenza investigation and vaccine testing, it is imperative that this model is recognized for its uses in prophylactic development and not only as an agent for creating transmissible influenza viruses. Elucidating the ferret immune response and creating ferret immune reagents remain important goals in conjunction with the development and manufacture of H5N1 vaccines. In summary, an efficacious H5N1 vaccine is urgently needed and the ferret model remains an appropriate model for its development. | Animals, Disease Models, Animal, Ferrets, Influenza A Virus, H5N1 Subtype, Influenza Vaccines, Orthomyxoviridae Infections | null |
22,706,188 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Genotypes and cephalosporin susceptibility in extended-spectrum beta-lactamase producing enterobacteriaceae in the community. | Maina Daniel, Revathi Gunturu, Kariuki Samuel, Ozwara Hastings | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Cephalosporins, beta-Lactamases | IM | 22706188, 10.3855/jidc.1456 | Infections from extended spectrum beta lactamases (ESBLs) producing enterobacteriaceae are increasingly being reported in the community setting. These infections are often multidrug resistant, with clinical and epidemiological implications, and necessitate surveillance measures based on local data. In the present study ESBLs genotypes were correlated with susceptibility to cephalosporins among ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates acquired in the community. | Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Cephalosporins, Child, Child, Preschool, Community-Acquired Infections, Cross-Sectional Studies, Escherichia coli, Escherichia coli Infections, Genotype, Humans, Klebsiella Infections, Klebsiella pneumoniae, Male, Microbial Sensitivity Tests, Middle Aged, Polymerase Chain Reaction, Young Adult, beta-Lactamases | null |
22,706,189 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Salmonella isolates serotypes and susceptibility to commonly used drugs at a tertiary care hospital in Riyadh, Saudi Arabia. | Somily Ali Mohammed, Sayyed Samina Bashir, Habib Hanan Ahmed, Al-Khattaf Abdulaziz Saleh, Al Otabi Fawzia Eida, Shakoor Zahid, Kambal Abdelmageed Mohammed | eng | null | Journal Article | Anti-Bacterial Agents | IM | 22706189, 10.3855/jidc.1805 | Resistance of Salmonella to therapeutic agents currently being used for treatment of Salmonella infections is emerging as a global problem. This study aimed to assess the prevalence of Salmonella serotypes and their susceptibility patterns to commonly used drugs for treatment of Salmonella infections including quinolones. Correlation between nalidixic acid susceptibility of these isolates and their ciprofloxacin minimum inhibitory concentrations was also sought. | Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Child, Child, Preschool, Drug Resistance, Bacterial, Female, Hospitals, Humans, Infant, Male, Microbial Sensitivity Tests, Middle Aged, Prevalence, Prospective Studies, Salmonella, Salmonella Infections, Saudi Arabia, Serotyping, Young Adult | null |
22,706,186 | 2013-06-25 | 2021-10-21 | 1872-9649 | Ageing research reviews | Organ-specific mediation of lifespan extension: more than a gut feeling? | Rera Michael, Azizi Masoud J, Walker David W | eng | R01 AG037514 (NIA NIH HHS, United States); R01 AG040288 (NIA NIH HHS, United States); R01AG040288, (NIA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | Electron Transport Chain Complex Proteins, Heat-Shock Proteins, Insulin, PPARGC1A protein, human, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Transcription Factors, Insulin-Like Growth Factor I | IM | 22706186, S1568-1637(12)00084-0, 10.1016/j.arr.2012.05.003, PMC3498542, NIHMS390091, 19797770, 4037084, 20926834, 20832466, 17090700, 14729567, 9252323, 18243090, 22053049, 20336132, 12845331, 19360127, 20346072, 21443682, 16054085, 21093262, 17988209, 21501374, 20089120, 9020081, 21115525, 20157545, 11675496, 15734678, 20056890, 12447374, 16285865, 21108726, 7789761, 22452899, 18940735, 11557972, 21325336, 9529258, 18691185, 8247153, 19918075, 17210671, 12136014, 8601482, 11292875, 18284659, 11557984, 21151885, 21215371, 9402931, 17465980, 18412985, 9360933, 15633135, 9353126, 19747824, 7768437, 12471266, 21195352, 19968629, 19910925, 3354681, 15192154, 8700226, 18635541, 18391175, 19371772, 11021802, 15565107, 17051205, 17056719, 11292874, 20976250, 21713031, 21518241, 17018277, 12588810, 16340960, 17914900, 10224252, 11972154, 15734681, 16340959, 3816482, 17538612, 17684529, 15175753, 11675495, 21629705, 17612391, 12750521, 9790527, 12397350, 15708981, 18358814, 11709184, 14622602, 2392681, 22055505, 9716135 | Multicellular organisms are composed of an interactive network of various tissues that are functionally organized as discrete organs. If aging were slowed in a specific tissue or organ how would that impact longevity at the organismal level? In recent years, molecular genetic approaches in invertebrate model systems have dramatically improved our understanding of the aging process and have provided insight into the preceding question. In this review, we discuss tissue and organ-specific interventions that prolong lifespan in the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster. These interventions include reduced Insulin/IGF-1 signaling, knockdown of genes important for mitochondrial electron transport chain function and, finally, up-regulation of the Drosophila PGC-1 homolog. An emerging theme from these studies is that the intestine is an important target organ in mediating lifespan extension at the organismal level. | Animals, Electron Transport Chain Complex Proteins, Heat-Shock Proteins, Humans, Insulin, Insulin-Like Growth Factor I, Intestines, Longevity, Mitochondria, Organ Specificity, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Signal Transduction, Transcription Factors | null |
22,706,190 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Emergence of an SGI1-bearing Salmonella enterica serotype Kentucky isolated from septic poultry in Nigeria. | Ogunleye Akinlabi O, Carlson Steve A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Virulence Factors | IM | 22706190, 10.3855/jidc.1988 | Salmonella enterica serotype Kentucky was isolated from septic poultry in Nigeria. The objective of this study was to characterize this isolate by screening for SGI1 and hyper-virulence. | Animals, Endocytosis, Genomic Islands, Nigeria, Polymerase Chain Reaction, Poultry, Poultry Diseases, Salmonella Infections, Animal, Salmonella enterica, Tetrahymena, Virulence, Virulence Factors | null |
22,706,191 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Frequency and antimicrobial resistance of bacteria isolated from oral and topical medicaments from Hilla, Iraq. | Al-Charrakh Alaa H | eng | null | Journal Article | Anti-Bacterial Agents | IM | 22706191, 10.3855/jidc.1817 | The presence of microorganisms in pharmaceuticals is undesirable because they may cause spoilage of the product and may present an infection hazard to the consumers or patients. | Anti-Bacterial Agents, Bacteria, Drug Contamination, Drug Resistance, Bacterial, Humans, Iraq, Microbial Sensitivity Tests | null |
22,706,192 | 2012-10-09 | 2022-03-11 | 1972-2680 | Journal of infection in developing countries | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? | Alsayed Yara, Monem Fawza | eng | null | Evaluation Study, Journal Article | null | IM | 22706192, 10.3855/jidc.2453 | Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. | Adolescent, Adult, Agglutination Tests, Brucella, Brucellosis, Child, Clinical Laboratory Techniques, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Polymerase Chain Reaction, Syria, Young Adult | null |
22,706,193 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Quorum sensing and virulence of Pseudomonas aeruginosa during urinary tract infections. | Senturk Sezgi, Ulusoy Seyhan, Bosgelmez-Tinaz Gulgun, Yagci Aysegul | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acyl-Butyrolactones, Virulence Factors, Pancreatic Elastase | IM | 22706193, 10.3855/jidc.2543 | In the opportunistic pathogen Pseudomonas aeruginosa, the production of several virulence factors depends on quorum sensing (QS) involving N-acylhomoserine lactone signal molecules. In vitro studies have suggested that the QS system is crucial in the pathogenesis of P. aeruginosa. However, it is unclear whether QS systems of P. aeruginosa play the same role during infections. | Acyl-Butyrolactones, Biofilms, Genes, Bacterial, Humans, Pancreatic Elastase, Polymerase Chain Reaction, Pseudomonas Infections, Pseudomonas aeruginosa, Quorum Sensing, Urinary Tract Infections, Virulence, Virulence Factors | null |
22,706,194 | 2012-10-09 | 2022-04-09 | 1972-2680 | Journal of infection in developing countries | Prevalence and factors associated with Group A rotavirus infection among children with acute diarrhea in Mwanza, Tanzania. | Temu Akwila, Kamugisha Erasmus, Mwizamholya Damas L, Hokororo Aldofina, Seni Jeremiah, Mshana Stephen E | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706194, 10.3855/jidc.1816 | Rotavirus infections frequently cause acute gastroenteritis in humans and are the most important cause of severe dehydrating diarrhea in young children in both developed and developing countries. | Child, Preschool, Communicable Disease Control, Cross-Sectional Studies, Diarrhea, Feces, Female, Gastroenteritis, Hospitals, Humans, Infant, Latex Fixation Tests, Length of Stay, Male, Prevalence, Prospective Studies, Risk Factors, Rotavirus, Rotavirus Infections, Tanzania | null |
22,706,197 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Saphenous neuropathy due to large hydatid cyst within long adductor muscle: case report and literature review. | Alimehmeti Ridvan, Seferi Arsen, Rroji Arben, Alimehmeti Mehdi | eng | null | Case Reports, Journal Article, Review | Anthelmintics, Albendazole | IM | 22706197, 10.3855/jidc.1766 | An unusual case of saphenous neuropathy secondary to compression by a large hydatid cyst within the adductor longus muscle is reported. Solitary hydatid cyst(s) localized in the skeletal muscles occur rarely and often mimic soft tissue tumours. Presentation with signs of peripheral nerve compression by a hydatid cyst in an extremity is exceedingly rare. Diagnosis can be established by ultrasound, computerized tomography or magnetic resonance if clinically suspected. Clinical suspicion of hydatid origin of a solitary muscle cyst should be high especially in patients hailing from areas endemic for echinococcosis. Laboratory tests are usually unhelpful in such cases and needle biopsy carries the risk of anaphylactic shock and should therefore be avoided. Surgical removal of the unruptured cyst is the treatment of choice in cases of intramuscular hydatid cyst. In the present case, excision of the hydatid cyst was followed by complete clinical recovery. In the absence of systemic involvement, treatment with albendazole may be avoided. | Adolescent, Albendazole, Anthelmintics, Echinococcosis, Femoral Neuropathy, Humans, Male, Muscular Diseases, Nerve Compression Syndromes, Tomography, X-Ray Computed | null |
22,706,195 | 2012-10-09 | 2019-11-12 | 1972-2680 | Journal of infection in developing countries | Pneumococcal disease: emergence of serotypes 19A and 7F following conjugate pneumococcal vaccination in a Mexican hospital. | Chacon-Cruz Enrique, Velazco-Mendez Yazbeck, Navarro-Alvarez Samuel, Rivas-Landeros Rosa M, Volker Maria Luisa, Lopez-Espinoza Graciano | eng | null | Journal Article | Heptavalent Pneumococcal Conjugate Vaccine, Pneumococcal Vaccines | IM | 22706195, 10.3855/jidc.1954 | Mexico was the country to initiate massive vaccination with heptavalent pneumococcal conjugate vaccine (PCV-7) in children. There is no information regarding pneumococcal invasive disease (PID) in children before and after implementation of PCV-7 in Mexico or elsewhere in Latin America. | Adolescent, Child, Child, Preschool, Empyema, Heptavalent Pneumococcal Conjugate Vaccine, Hospitals, Pediatric, Humans, Infant, Male, Mastoiditis, Meningitis, Bacterial, Mexico, Pneumococcal Infections, Pneumococcal Vaccines, Serotyping, Streptococcus pneumoniae | null |
22,706,196 | 2012-10-09 | 2022-04-19 | 1972-2680 | Journal of infection in developing countries | Two cases of bacteremia due to an unusual pathogen, Comamonas testosteroni in Iran and a review literature. | Farshad Shohreh, Norouzi Fatemeh, Aminshahidi Maneli, Heidari Behzad, Alborzi Abdolvahab | eng | null | Case Reports, Journal Article, Review | Anti-Bacterial Agents | IM | 22706196, 10.3855/jidc.2215 | Here we describe two cases of bacteremia caused by Comamonas testosteroni in two malignant patients, a 10-year-old boy with brain medulloblastoma and a 19-year-old girl with osteosarcoma admitted in the same hospital at short intervals. This is the first report in Iran on this low inherent virulence organism as a human pathogen. | Anti-Bacterial Agents, Bacteremia, Child, Comamonas testosteroni, Female, Gram-Negative Bacterial Infections, Humans, Iran, Male, Neoplasms, Treatment Outcome, Young Adult | null |
22,706,198 | 2012-10-09 | 2019-12-10 | 1972-2680 | Journal of infection in developing countries | A new method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte. | Ferru Emanuela, Pantaleo Anntonella, Turrini Francesco | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Validation Study | Membrane Proteins, Protozoan Proteins, Biotin | IM | 22706198, 10.3855/jidc.2386 | We propose a new method for the selective labeling, isolation and electrophoretic analysis of the Plasmodium falciparum protein exposed on the erythrocyte cell surface. Historically, membrane surface proteins have been isolated using a surface biotinylation followed by capture of biotin-conjugated protein via an avidin/streptavidin-coated solid support. The major drawback of the standard methods has been the labeling of internal proteins due to fast internalization of biotin. | Adult, Biotin, Erythrocytes, Female, Humans, Male, Mass Spectrometry, Membrane Proteins, Parasitology, Plasmodium falciparum, Protozoan Proteins, Sensitivity and Specificity, Staining and Labeling | null |
22,706,199 | 2012-11-05 | 2021-10-21 | 1552-4469 | Nature chemical biology | Lys34 of translation elongation factor EF-P is hydroxylated by YfcM. | Peil Lauri, Starosta Agata L, Virumäe Kai, Atkinson Gemma C, Tenson Tanel, Remme Jaanus, Wilson Daniel N | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Escherichia coli Proteins, Peptide Elongation Factors, factor EF-P, Mixed Function Oxygenases, YfcM protein, E coli, Chymotrypsin, Lysine | IM | 22706199, nchembio.1001, 10.1038/nchembio.1001, 17042480, 20070887, 19696344, 20729861, 20670890, 16597989, 18201202, 21841797, 21348575, 1105576, 9419357, 383483, 16928980, 10987361, 19997760, 22128152, 17896105, 9826331, 11114898 | Lys34 of the conserved translation elongation factor P (EF-P) is post-translationally lysinylated by YjeK and YjeA--a modification that is critical for bacterial virulence. Here we show that the currently accepted Escherichia coli EF-P modification pathway is incomplete and lacks a final hydroxylation step mediated by YfcM, an enzyme distinct from deoxyhypusine hydroxylase that catalyzes the final maturation step of eukaryotic initiation factor 5A, the eukaryotic EF-P homolog. | Chymotrypsin, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Lysine, Mass Spectrometry, Mixed Function Oxygenases, Molecular Structure, Peptide Elongation Factors, Protein Binding, Protein Processing, Post-Translational | null |
22,706,202 | 2012-12-04 | 2013-11-21 | 1538-7445 | Cancer research | Caveolin-1 increases aerobic glycolysis in colorectal cancers by stimulating HMGA1-mediated GLUT3 transcription. | Ha Tae-Kyu, Her Nam-Gu, Lee Min-Goo, Ryu Byung-Kyu, Lee Jin-Hee, Han Jikhyon, Jeong Seong-In, Kang Min-Ju, Kim Nam-Hoon, Kim Hyo-Jong, Chi Sung-Gil | eng | null | Journal Article, Research Support, Non-U.S. Gov't | CAV1 protein, human, Caveolin 1, Glucose Transporter Type 3, SLC2A3 protein, human, Tumor Suppressor Protein p53, HMGA1a Protein, Adenosine Triphosphate, Adenylate Kinase, Glucose | IM | 22706202, 0008-5472.CAN-12-0448, 10.1158/0008-5472.CAN-12-0448 | Caveolin-1 (CAV1) acts as a growth suppressor in various human malignancies, but its expression is elevated in many advanced cancers, suggesting the oncogenic switch of its role during tumor progression. To understand the molecular basis for the growth-promoting function of CAV1, we characterized its expression status, differential roles for tumor growth, and effect on glucose metabolism in colorectal cancers. Abnormal elevation of CAV1 was detected in a substantial fraction of primary tumors and cell lines and tightly correlated with promoter CpG sites hypomethylation. Depletion of elevated CAV1 led to AMPK activation followed by a p53-dependent G1 cell-cycle arrest and autophagy, suggesting that elevated CAV1 may contribute to ATP generation. Furthermore, CAV1 depletion downregulated glucose uptake, lactate accumulation, and intracellular ATP level, supporting that aerobic glycolysis is enhanced by CAV1. Consistently, CAV1 was shown to stimulate GLUT3 transcription via an HMGA1-binding site within the GLUT3 promoter. HMGA1 was found to interact with and activate the GLUT3 promoter and CAV1 increased the HMGA1 activity by enhancing its nuclear localization. Ectopic expression of HMGA1 increased glucose uptake, whereas its knockdown caused AMPK activation. In addition, GLUT3 expression was strongly induced by cotransfection of CAV1 and HMGA1, and its overexpression was observed predominantly in tumors harboring high levels of CAV1 and HMGA1. Together, these data show that elevated CAV1 upregulates glucose uptake and ATP production through HMGA1-mediated GLUT3 transcription, suggesting that CAV1 may render tumor cells growth advantages by enhancing aerobic glycolysis. | Adenosine Triphosphate, Adenylate Kinase, Aerobiosis, Animals, Autophagy, Caveolin 1, Cell Line, Tumor, Colorectal Neoplasms, DNA Methylation, Female, Glucose, Glucose Transporter Type 3, Glycolysis, HCT116 Cells, HMGA1a Protein, Humans, Immunoblotting, Mice, Mice, Nude, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic, Tumor Suppressor Protein p53, Up-Regulation | null |
22,706,201 | 2012-09-10 | 2012-07-03 | 1538-7445 | Cancer research | MAX and MYC: a heritable breakup. | Cascón Alberto, Robledo Mercedes | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, MAX protein, human | IM | 22706201, 0008-5472.CAN-11-3891, 10.1158/0008-5472.CAN-11-3891 | The overexpression of MYC, which occurs in many tumors, dramatically disrupts the equilibrium between activation and repression of the oncogenic MYC/MYC-associated protein X (MAX)/MAX dimerization protein 1 (MXD1) network, favoring MYC-MAX complexes and thereby impairing differentiation and promoting cell growth. Although for some time it has appeared that MAX is necessary for both the activation and repression of the axis, recent evidence shows that MYC retains considerable biologic function in the absence of MAX. The presence of germline MAX mutations in patients with hereditary pheochromocytoma supports the predominant role of MAX as a negative regulator of the network and suggests that MYC deregulation plays a role in hereditary cancer predisposition. This finding also confirms the importance of impairment of the MYC/MAX/MXD1 axis in the development of aggressive neural tumors, because MYCN overexpression is an established genetic hallmark of malign neuroblastoma, and it is likely that MXI1 plays a relevant role in the development of medulloblastoma and glioblastoma. Finally, the likely malignant behavior of tumors with mutations in MAX points to MYC as a candidate therapeutic target in the treatment of metastatic pheochromocytoma. | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Genes, myc, Germ-Line Mutation, Humans, Neoplasms | null |
22,706,200 | 2012-11-05 | 2022-03-31 | 1552-4469 | Nature chemical biology | Phenylalanine assembly into toxic fibrils suggests amyloid etiology in phenylketonuria. | Adler-Abramovich Lihi, Vaks Lilach, Carny Ohad, Trudler Dorit, Magno Andrea, Caflisch Amedeo, Frenkel Dan, Gazit Ehud | eng | G1100695 (Medical Research Council, United Kingdom) | Journal Article, Research Support, Non-U.S. Gov't | Amyloid, Antibodies, Phenylalanine | IM | 22706200, nchembio.1002, 10.1038/nchembio.1002, 8375656, 17918282, 3700406, 19444816, 16549526, 17501694, 11932737, 12714741, 3880775, 6684021, 10408445, 19935655, 11772939, 15465508, 12842290, 16302960, 16541076, 16339876, 20373144, 10679462, 16756495, 9662374, 15630094, 12095997, 8621479, 12075024, 11043156, 12124613 | Phenylketonuria (PKU) is characterized by phenylalanine accumulation and progressive mental retardation caused by an unknown mechanism. We demonstrate that at pathological concentrations, phenylalanine self-assembles into fibrils with amyloid-like morphology and well-ordered electron diffraction. These assemblies are specifically recognized by antibodies, show cytotoxicity that can be neutralized by the antibodies and are present in the hippocampus of model mice and in parietal cortex brain tissue from individuals with PKU. This is, to our knowledge, the first demonstration that a single amino acid can form amyloid-like deposits, suggesting a new amyloidosis-like etiology for PKU. | Amyloid, Animals, Antibodies, Biophysics, CHO Cells, Cricetinae, Hippocampus, Humans, Immunoprecipitation, Mice, Models, Molecular, Parietal Lobe, Phenylalanine, Phenylketonurias, Protein Conformation, Rabbits | null |
22,706,204 | 2013-04-05 | 2013-11-21 | 1879-176X | Journal of dentistry | Effects of fluoride concentration and temperature of milk on caries lesion rehardening. | Lippert F, Martinez-Mier E A, Soto-Rojas A E | eng | null | Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | Cariostatic Agents, Saliva, Artificial, Fluorides | IM | 22706204, S0300-5712(12)00146-7, 10.1016/j.jdent.2012.06.001 | The aim of the present in vitro study was to investigate the effects of fluoride concentration and temperature of milk on caries lesion rehardening under pH cycling conditions. | Animals, Cariostatic Agents, Dental Caries, Dental Enamel, Dose-Response Relationship, Drug, Fluorides, Hardness, Humans, Hydrogen-Ion Concentration, Milk, Saliva, Saliva, Artificial, Temperature, Tooth Remineralization | null |
22,706,203 | 2012-12-04 | 2012-08-16 | 1538-7445 | Cancer research | The retinoblastoma gene undergoes rearrangements in BRCA1-deficient basal-like breast cancer. | Jönsson Göran, Staaf Johan, Vallon-Christersson Johan, Ringnér Markus, Gruvberger-Saal Sofia K, Saal Lao H, Holm Karolina, Hegardt Cecilia, Arason Adalgeir, Fagerholm Rainer, Persson Camilla, Grabau Dorthe, Johnsson Ellinor, Lövgren Kristina, Magnusson Linda, Heikkilä Päivi, Agnarsson Bjarni A, Johannsson Oskar T, Malmström Per, Fernö Mårten, Olsson Håkan, Loman Niklas, Nevanlinna Heli, Barkardottir Rosa B, Borg Åke | eng | null | Journal Article, Research Support, Non-U.S. Gov't | BRCA1 Protein, BRCA1 protein, human, Retinoblastoma Protein | IM | 22706203, 0008-5472.CAN-12-0097, 10.1158/0008-5472.CAN-12-0097 | Breast tumors from BRCA1 germ line mutation carriers typically exhibit features of the basal-like molecular subtype. However, the specific genes recurrently mutated as a consequence of BRCA1 dysfunction have not been fully elucidated. In this study, we used gene expression profiling to molecularly subtype 577 breast tumors, including 73 breast tumors from BRCA1/2 mutation carriers. Focusing on the RB1 locus, we analyzed 33 BRCA1-mutated, 36 BRCA2-mutated, and 48 non-BRCA1/2-mutated breast tumors using a custom-designed high-density oligomicroarray covering the RB1 gene. We found a strong association between the basal-like subtype and BRCA1-mutated breast tumors and the luminal B subtype and BRCA2-mutated breast tumors. RB1 was identified as a major target for genomic disruption in tumors arising in BRCA1 mutation carriers and in sporadic tumors with BRCA1 promoter methylation but rarely in other breast cancers. Homozygous deletions, intragenic breaks, or microdeletions were found in 33% of BRCA1-mutant tumors, 36% of BRCA1 promoter-methylated basal-like tumors, 13% of non-BRCA1-deficient basal-like tumors, and 3% of BRCA2-mutated tumors. In conclusion, RB1 was frequently inactivated by gross gene disruption in BRCA1 hereditary breast cancer and BRCA1-methylated sporadic basal-like breast cancer but rarely in BRCA2 hereditary breast cancer and non-BRCA1-deficient sporadic breast cancers. Together, our findings show the existence of genetic heterogeneity within the basal-like breast cancer subtype that is based upon BRCA1 status. | BRCA1 Protein, Breast Neoplasms, Cell Line, Tumor, Comparative Genomic Hybridization, Female, Gene Dosage, Gene Rearrangement, Genes, BRCA1, Genes, Retinoblastoma, Germ-Line Mutation, Humans, In Situ Hybridization, Fluorescence, Neoplasms, Basal Cell, Retinoblastoma Protein, Transcriptome | null |
22,706,205 | 2012-11-27 | 2013-11-21 | 1756-591X | Metallomics : integrated biometal science | Proteomic analysis of proteins secreted by Botrytis cinerea in response to heavy metal toxicity. | Cherrad Semcheddine, Girard Vincent, Dieryckx Cindy, Gonçalves Isabelle R, Dupuy Jean-William, Bonneu Marc, Rascle Christine, Job Claudette, Job Dominique, Vacher Sébastien, Poussereau Nathalie | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Environmental Pollutants, Fungal Proteins, Cadmium, Copper, Nickel, Oxidoreductases, Peptide Hydrolases, Zinc | IM | 22706205, 10.1039/c2mt20041d | Although essential in many cellular processes, metals become toxic when they are present in excess and constitute a global environmental hazard. To overcome this stress, fungi have evolved several mechanisms at both intracellular and extracellular levels. In particular, fungi are well known for their ability to secrete a large panel of proteins. However, their role in the adaptation of fungi to metal toxicity has not yet been investigated. To address this question, here, the fungus Botrytis cinerea was challenged to copper, zinc, nickel or cadmium stress and secreted proteins were collected and separated by 2D-PAGE. One hundred and sixteen spots whose volume varied under at least one tested condition were observed on 2D gels. Densitometric analyses revealed that the secretome signature in response to cadmium was significantly different from those obtained with the other metals. Fifty-five of these 116 spots were associated with unique proteins and functional classification revealed that the production of oxidoreductases and cell-wall degrading enzymes was modified in response to metals. Promoter analysis disclosed that PacC/Rim101 sites were statistically over-represented in the upstream sequences of the 31 genes corresponding to the varying unique spots suggesting a possible link between pH regulation and metal response in B. cinerea. | Botrytis, Cadmium, Copper, Electrophoresis, Gel, Two-Dimensional, Environmental Pollutants, Fungal Proteins, Gene Expression Regulation, Fungal, Nickel, Oxidoreductases, Peptide Hydrolases, Proteomics, Zinc | null |
22,706,208 | 2012-12-13 | 2013-11-21 | 1095-8274 | Anaerobe | Bacterioferritin protects the anaerobe Desulfovibrio vulgaris Hildenborough against oxygen. | Figueiredo Mafalda C O, Lobo Susana A L, Carita João N, Nobre Lígia S, Saraiva Lígia M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Bacterial Proteins, Cytochrome b Group, Reactive Oxygen Species, Repressor Proteins, Ferritins, bacterioferritin, Oxygen | IM | 22706208, S1075-9964(12)00093-5, 10.1016/j.anaerobe.2012.06.001 | Intracellular free iron, is under aerobic conditions and via the Fenton reaction a catalyst for the formation of harmful reactive oxygen species. In this article, we analyzed the relation between intracellular iron storage and oxidative stress response in the sulfate reducing bacterium Desulfovibrio vulgaris Hildenborough, an anaerobe that is often found in oxygenated niches. To this end, we investigated the role of the iron storage protein bacterioferritin using transcriptomic and physiological approaches. We observed that transcription of bacterioferritin is strongly induced upon exposure of cells to an oxygenated atmosphere. When grown in the presence of high concentrations of oxygen the D. vulgaris bacterioferritin mutant exhibited, in comparison with the wild type strain, lower viability and a higher content of intracellular reactive oxygen species. Furthermore, the bacterioferritin gene is under the control of the oxidative stress response regulator D. vulgaris PerR. Altogether the data revealed a previously unrecognized ability for the iron storage bacterioferritin to contribute to the oxygen tolerance exhibited by D. vulgaris. | Adaptation, Physiological, Bacterial Proteins, Cytochrome b Group, Desulfovibrio vulgaris, Ferritins, Gene Expression Regulation, Bacterial, Genes, Bacterial, Microbial Viability, Oxidative Stress, Oxygen, Reactive Oxygen Species, Repressor Proteins, Time Factors, Transcription, Genetic | null |
22,706,210 | 2012-09-26 | 2023-08-10 | 1537-4521 | Sexually transmitted diseases | Notification for sexually transmitted infections and HIV among sex workers in Guatemala: acceptability, barriers, and preferences. | Sabidó Meritxell, Gregg Lucile Parker, Vallès Xavier, Nikiforov Mikhail, Monzón Jose Ernesto, Pedroza Maria Isabel, Vermund Sten H, Casabona Jordi | eng | P30 AI110527 (NIAID NIH HHS, United States) | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706210, 10.1097/OLQ.0b013e31824f8b1b, 00007435-201207000-00003 | Partner notification for HIV and other sexually transmitted infections is acceptable and feasible among female sex workers attending sexually transmitted infection clinics in Guatemala, especially for regular partners. Intention to refer the sexual partner was best predicted by attitude followed by social norms and baby's protection. Women preferred notification via patient-based referral. | Adult, Contact Tracing, Female, Guatemala, HIV Seropositivity, Humans, Infant, Patient Preference, Pregnancy, Referral and Consultation, Sex Workers, Sexual Partners, Sexually Transmitted Diseases | null |
22,706,206 | 2012-12-05 | 2013-11-21 | 1477-9234 | Dalton transactions (Cambridge, England : 2003) | Keggin polyoxoanion supported organic-inorganic trinuclear lutetium cluster, {Na(H2O)3[Lu(pydc)(H2O)3]3}[SiW12O40]·26.5H2O. | Li Suzhi, Zhang Dongdi, Guo Yuan Yuan, Ma Pengtao, Qiu Xiaoyang, Wang Jingping, Niu Jingyang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Coordination Complexes, Tungsten Compounds, polyoxometalate I, Lutetium, Tungsten, Silicon | IM | 22706206, 10.1039/c2dt30517h | A novel strawberry-like organic-inorganic hybrid, {Na(H(2)O)(3)[Lu(pydc)(H(2)O)(3)](3)}[SiW(12)O(40)]·26.5H(2)O (H(2)pydc = pyridine-2,6-dicarboxylate) containing an intriguing trinuclear lutetium cluster {Na(H(2)O)(3)[Lu(pydc)(H(2)O)(3)](3)}(4+) has been synthesized and its luminescent properties, IR, UV, TG, PXRD analyses and single crystal X-ray diffraction were investigated. | Coordination Complexes, Crystallography, X-Ray, Lutetium, Molecular Conformation, Silicon, Spectrophotometry, Ultraviolet, Tungsten, Tungsten Compounds | null |
22,706,209 | 2012-12-13 | 2013-11-21 | 0027-5107 | Mutation research | Combined exposure to X-irradiation followed by N-ethyl-N-nitrosourea treatment alters the frequency and spectrum of Ikaros point mutations in murine T-cell lymphoma. | Kakinuma Shizuko, Nishimura Mayumi, Amasaki Yoshiko, Takada Mayumi, Yamauchi Kazumi, Sudo Satomi, Shang Yi, Doi Kazutaka, Yoshinaga Shinji, Shimada Yoshiya | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Carcinogens, Zfpn1a1 protein, mouse, Ikaros Transcription Factor, DNA Repair Enzymes, Ethylnitrosourea | IM | 22706209, S0027-5107(12)00130-3, 10.1016/j.mrfmmm.2012.06.001 | Ionizing radiation is a well-known carcinogen, but its potency may be influenced by other environmental carcinogens, which is of practical importance in the assessment of risk. Data are scarce, however, on the combined effect of radiation with other environmental carcinogens and the underlying mechanisms involved. We studied the mode and mechanism of the carcinogenic effect of radiation in combination with N-ethyl-N-nitrosourea (ENU) using doses approximately equal to the corresponding thresholds. B6C3F1 mice exposed to fractionated X-irradiation (Kaplan's method) followed by ENU developed T-cell lymphomas in a dose-dependent manner. Radiation doses above an apparent threshold acted synergistically with ENU to promote lymphoma development, whereas radiation doses below that threshold antagonized lymphoma development. Ikaros, which regulates the commitment and differentiation of lymphoid lineage cells, is a critical tumor suppressor gene frequently altered in both human and mouse lymphomas and shows distinct mutation spectra between X-ray- and ENU-induced lymphomas. In the synergistically induced lymphomas, we observed a low frequency of LOH and an inordinate increase of Ikaros base substitutions characteristic of ENU-induced point mutations, G:C to A:T at non-CpG, A:T to G:C, G:C to T:A and A:T to T:A. This suggests that radiation doses above an apparent threshold activate the ENU mutagenic pathway. This is the first report on the carcinogenic mechanism elicited by combined exposure to carcinogens below and above threshold doses based on the mutation spectrum of the causative gene. These findings constitute a basis for assessing human cancer risk following exposure to multiple carcinogens. | Animals, Carcinogens, DNA Repair Enzymes, Dose-Response Relationship, Radiation, Ethylnitrosourea, Female, Ikaros Transcription Factor, Loss of Heterozygosity, Lymphoma, T-Cell, Mice, Point Mutation, X-Rays | null |
22,706,211 | 2012-09-26 | 2024-06-10 | 1537-4521 | Sexually transmitted diseases | Postpartum sexually transmitted disease: refining our understanding of the population at risk. | Wheeler Rebekah, Earnshaw Valerie A, Kershaw Trace, Ickovics Jeannette R | eng | R01MH/HD61175 (NICHD NIH HHS, United States); T32 MH020031-12 (NIMH NIH HHS, United States); T32-MH20031 (NIMH NIH HHS, United States); F32 HD061175 (NICHD NIH HHS, United States); T32 MH020031 (NIMH NIH HHS, United States); R01-MH074399 (NIMH NIH HHS, United States); R01 MH074399-05 (NIMH NIH HHS, United States); R01 MH074399 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22706211, 10.1097/OLQ.0b013e31824e5167, 00007435-201207000-00004, PMC3942157, NIHMS361662, 7805634, 8712272, 17666608, 7645632, 19698033, 18578875, 9648047, 18426286, 8827145, 15723771, 10225587, 7889904, 12218839, 14663123, 17339505, 18987617, 14596957, 19762662 | Scant literature exists on sexually transmitted disease (STD) risk specific to pregnancy and postpartum. The objective of this prospective study is to examine risk factors for incident postpartum STDs among young mothers (ages, 14-25). | Adolescent, Adult, Educational Status, Female, Health Knowledge, Attitudes, Practice, Humans, Infant, Newborn, Postpartum Period, Pregnancy, Pregnancy Complications, Infectious, Pregnancy in Adolescence, Prenatal Care, Prevalence, Prospective Studies, Risk Factors, Sexual Behavior, Sexual Partners, Sexually Transmitted Diseases, Socioeconomic Factors, Substance-Related Disorders, United States, Young Adult | null |
22,706,212 | 2012-09-26 | 2019-12-10 | 1537-4521 | Sexually transmitted diseases | Comparison of the BD Viper System with XTR Technology to the Gen-Probe APTIMA COMBO 2 Assay using the TIGRIS DTS system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens. | Mushanski Linda M, Brandt Ken, Coffin Nicolette, Levett Paul N, Horsman Gregory B, Rank Elliot L | eng | null | Comparative Study, Evaluation Study, Journal Article | Reagent Kits, Diagnostic | IM | 22706212, 10.1097/OLQ.0b013e31824f2f5b, 00007435-201207000-00005 | Performances of the BD ProbeTec Chlamydia trachomatis (CT)/Neisseria Gonorrhoeae (GC) Q(x) Amplified DNA Assay reagents on a BD Viper System with XTR Technology and APTIMA COMBO 2 Assay reagents on a TIGRIS DTS platform, for detection of both CT and GC were compared. | Algorithms, Automation, Laboratory, Bacterial Typing Techniques, Chlamydia Infections, Chlamydia trachomatis, Female, Gonorrhea, Humans, Male, Molecular Diagnostic Techniques, Neisseria gonorrhoeae, Nucleic Acid Amplification Techniques, Reagent Kits, Diagnostic, Saskatchewan, Sensitivity and Specificity | null |
22,706,214 | 2012-09-26 | 2012-06-18 | 1537-4521 | Sexually transmitted diseases | Impact of reverse sequence syphilis screening on new diagnoses of late latent syphilis in Edmonton, Canada. | Gratrix Jennifer, Plitt Sabrina, Lee Bonita E, Ferron Leslie, Anderson Barbara, Verity Bob, Prasad Errol, Bunyan Roxanne, Zahariadis George, Singh Ameeta E | eng | null | Journal Article | null | IM | 22706214, 10.1097/OLQ.0b013e31824e53f7, 00007435-201207000-00007 | After the introduction of reverse sequence syphilis screening in Alberta, Canada, there was an increase in the diagnosis of late latent syphilis in individuals screening positive with the treponemal test; these cases required additional public health follow-up. | Adult, Alberta, Contact Tracing, Female, Fluorescent Treponemal Antibody-Absorption Test, Humans, Immunoenzyme Techniques, Infectious Disease Transmission, Vertical, Male, Mass Screening, Middle Aged, Prevalence, Sensitivity and Specificity, Syphilis Serodiagnosis, Syphilis, Latent, Treponema pallidum | null |
22,706,215 | 2012-09-26 | 2012-06-18 | 1537-4521 | Sexually transmitted diseases | Urethritis/cervicitis pathogen prevalence and associated risk factors among asymptomatic HIV-infected patients in South Africa. | Lewis David A, Chirwa Tobias F, Msimang Veerle M Y, Radebe Frans M, Kamb Mary L, Firnhaber Cynthia S | eng | U62/CCU022901 (PHS HHS, United States) | Journal Article, Research Support, U.S. Gov't, P.H.S. | null | IM | 22706215, 10.1097/OLQ.0b013e31824cbecc, 00007435-201207000-00008 | To determine sexually transmitted infection (STI) prevalence, and patient characteristics associated with detection of urethritis/cervicitis pathogens, among HIV-infected individuals offered voluntary STI screening at a South African HIV treatment center. | AIDS-Related Opportunistic Infections, Adult, Aged, Algorithms, CD4 Lymphocyte Count, Chlamydia trachomatis, Cross-Sectional Studies, Female, HIV Seropositivity, Humans, Male, Mass Screening, Middle Aged, Mycoplasma genitalium, Neisseria gonorrhoeae, Polymerase Chain Reaction, Prevalence, Risk Factors, Sexual Partners, South Africa, Trichomonas vaginalis, Urethritis, Uterine Cervicitis, Viral Load | null |
22,706,213 | 2012-09-26 | 2019-12-10 | 1537-4521 | Sexually transmitted diseases | Recreational drug use during sex and sexually transmitted infections among clients of a city sexually transmitted infections clinic in Amsterdam, the Netherlands. | Heiligenberg Marlies, Wermeling Paulien R, van Rooijen Martijn S, Urbanus Anouk T, Speksnijder Arjen G C L, Heijman Titia, Prins Maria, Coutinho Roel A, van der Loeff Maarten F Schim | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Illicit Drugs | IM | 22706213, 10.1097/OLQ.0b013e3182515601, 00007435-201207000-00006 | Recreational drug use is associated with high-risk sexual behavior and sexually transmitted infections (STIs). We assessed the prevalence of drug use during sex and the associations between such use and STI (chlamydia, gonorrhea, or syphilis). | Adolescent, Adult, Coitus, Educational Status, Female, Humans, Illicit Drugs, Male, Netherlands, Prevalence, Sexual Behavior, Sexual Partners, Sexually Transmitted Diseases, Substance-Related Disorders, Surveys and Questionnaires, Unsafe Sex, Urban Population, Young Adult | null |
22,706,216 | 2012-09-26 | 2022-12-07 | 1537-4521 | Sexually transmitted diseases | Variation in concurrent sexual partnerships and sexually transmitted diseases among African men in Cape Town, South Africa. | Maughan-Brown Brendan | eng | null | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | null | IM | 22706216, 10.1097/OLQ.0b013e31824cc0c1, 00007435-201207000-00009 | Most studies that assess the relationship between concurrent sexual partnerships and sexually transmitted diseases (STDs) use dichotomous measures of whether concurrency was reported or not. However, different forms of concurrency have different degrees of associated risk for disease transmission, and this should be considered. This article examines variation in both individual concurrency and partner concurrency among African men in Cape Town, South Africa, and assesses the relationship between different types of concurrent partnerships and STDs. | Adolescent, Analysis of Variance, Black People, Female, Humans, Male, Models, Theoretical, Prevalence, Risk Factors, Sexual Behavior, Sexual Partners, Sexually Transmitted Diseases, South Africa, Surveys and Questionnaires, Young Adult | null |
22,706,218 | 2012-09-26 | 2018-12-01 | 1537-4521 | Sexually transmitted diseases | Sexual event-level characteristics of condom use during anal intercourse among HIV-negative men who have sex with men. | Hensel Devon J, Rosenberger Joshua G, Novak David S, Reece Michael | eng | null | Journal Article | null | IM | 22706218, 10.1097/OLQ.0b013e31824f1da4, 00007435-201207000-00011 | Condom use remains central to sexually transmitted infections/HIV prevention among gay men and other men who have sex with men (MSM). To support the development of accurate and appropriate interventions, a better understanding is needed as to how the characteristics of a given sexual event differentially influence condom use during anal intercourse. | Adolescent, Adult, Condoms, Homosexuality, Male, Humans, Longitudinal Studies, Male, Middle Aged, Risk Factors, Sexual Behavior, Sexual Partners, Sexually Transmitted Diseases | null |
22,706,219 | 2012-09-26 | 2014-04-08 | 1537-4521 | Sexually transmitted diseases | Genotypic characterization of Trichomonas vaginalis isolates among women who have sex with women in sexual partnerships. | Muzny Christina A, Rivers Charles A, Mena Leandro A, Schwebke Jane R | eng | null | Journal Article | DNA, Protozoan | IM | 22706219, 10.1097/OLQ.0b013e31824f1c49, 00007435-201207000-00012 | The random amplified polymorphic DNA technique was used to delineate the genetic relatedness of Trichomonas vaginalis isolates among 3 pairs of mutually infected women who have sex with women in sexual partnerships. One of the 3 pairs of women shared a T. vaginalis isolate with the same random amplified polymorphic DNA banding patterns. Shared use of washcloths to cleanse the vaginal area after receptive oral sex was the most likely method of T. vaginalis transmission among this pair of women. | DNA, Protozoan, Female, Genotype, Health Knowledge, Attitudes, Practice, Homosexuality, Female, Humans, Phylogeny, Random Amplified Polymorphic DNA Technique, Sexual Behavior, Sexual Partners, Sexually Transmitted Diseases, Trichomonas Vaginitis, Trichomonas vaginalis | null |
22,706,220 | 2012-09-26 | 2012-06-18 | 1537-4521 | Sexually transmitted diseases | The prevalence and incidence of oral human papillomavirus infection among young men and women, aged 18-30 years. | Pickard Robert K L, Xiao Weihong, Broutian Tatevik R, He Xin, Gillison Maura L | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706220, 10.1097/OLQ.0b013e31824f1c65, 00007435-201207000-00013 | Oral human papillomavirus (HPV) infection is a cause of oropharyngeal squamous cell carcinoma, yet little is known about the epidemiology and natural history of infection. | Adolescent, Adult, Carcinoma, Squamous Cell, Female, Human papillomavirus 16, Humans, Incidence, Male, Multiplex Polymerase Chain Reaction, Oropharyngeal Neoplasms, Papillomavirus Infections, Precancerous Conditions, Prevalence, Risk Factors, Sexual Behavior, Sexual Partners, Substance-Related Disorders, Young Adult | null |
22,706,217 | 2012-09-26 | 2019-12-10 | 1537-4521 | Sexually transmitted diseases | Evaluation of the Roche cobas® CT/NG test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in male urine. | Taylor Stephanie N, Liesenfeld Oliver, Lillis Rebecca A, Body Barbara A, Nye Melinda, Williams James, Eisenhut Carol, Hook Edward W, Van Der Pol Barbara | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | DNA, Bacterial | IM | 22706217, 10.1097/OLQ.0b013e31824e26ff, 00007435-201207000-00010 | The Roche cobas® CT/NG test (c4800), performed on the cobas 4800 system, is a new diagnostic assay using an automated workstation to isolate nucleic acids from clinical specimens and a real-time instrument for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG). This study compared the performance characteristics of the c4800 with the Becton Dickinson ProbeTec™ CT/GC Q(x) assay (Q(x)) and Gen-Probe® Aptima Combo 2 (AC2) assay for the detection of CT and NG in male urine using patient-infected-status (PIS). | Adolescent, Adult, Chlamydia Infections, Chlamydia trachomatis, DNA, Bacterial, Gonorrhea, Humans, Male, Middle Aged, Neisseria gonorrhoeae, Nucleic Acid Amplification Techniques, Sensitivity and Specificity, Urethra, Young Adult | null |
22,706,222 | 2012-10-16 | 2012-06-29 | 1464-0333 | Journal of environmental monitoring : JEM | Emerging investigators themed issue 2012. | Cwiertny David, Kohn Tamar | eng | null | Editorial, Introductory Journal Article | null | IM | 22706222, 10.1039/c2em90030k | null | Ecology, Environmental Monitoring, Humans | null |
22,706,221 | 2012-09-26 | 2024-04-12 | 1537-4521 | Sexually transmitted diseases | Male circumcision for HIV prevention: clinical practices and attitudes among healthcare providers in South Africa and Zimbabwe. | Sheldon Wendy R, Nhemachena Taazadza, Blanchard Kelly, Chipato Tsungai, Ramjee Gita, Trussell James, McCulloch Charles E, Blum Maya, Harper Cynthia C | eng | T32 HD007163-26 (NICHD NIH HHS, United States); R01 HD046027-01 (NICHD NIH HHS, United States); R24 HD047879-01 (NICHD NIH HHS, United States); T32HD007163 (NICHD NIH HHS, United States); T32 HD007163 (NICHD NIH HHS, United States); R24HD047879 (NICHD NIH HHS, United States); R24 HD047879 (NICHD NIH HHS, United States); R01 HD046027 (NICHD NIH HHS, United States) | Journal Article, Multicenter Study, Research Support, N.I.H., Extramural | null | IM | 22706221, 10.1097/OLQ.0b013e31824f9eaf, 00007435-201207000-00014, PMC3377943, NIHMS363527, 22112602, 21216000, 16101590, 11948269, 19707071, 20558024, 17321321, 22140364, 12697800, 19828190, 17053855, 15121412, 19321868, 18976733, 17321311, 20618752, 22140365, 16231970, 19086814, 18064746, 17321310, 17574662, 18261501 | This study aimed to document the clinical practices and attitudes of health care providers in South Africa and Zimbabwe on male circumcision for human immunodeficiency virus (HIV) prevention. | Acquired Immunodeficiency Syndrome, Adult, Circumcision, Male, Counseling, Cultural Characteristics, Delivery of Health Care, Health Knowledge, Attitudes, Practice, Humans, Male, Nurses, Physicians, Practice Patterns, Physicians', Risk Factors, Risk-Taking, South Africa, Surveys and Questionnaires, Zimbabwe | null |
22,706,224 | 2012-11-08 | 2018-10-30 | 1525-1438 | International journal of gynecological cancer : official journal of the International Gynecological Cancer Society | Expression patterns of kinin-dependent genes in endometrial cancer. | Orchel Joanna, Witek Lukasz, Kimsa Malgorzata, Strzalka-Mrozik Barbara, Kimsa Magdalena, Olejek Anita, Mazurek Urszula | eng | null | Journal Article, Research Support, Non-U.S. Gov't | RNA, Messenger, Receptor, Bradykinin B1, Receptor, Bradykinin B2 | IM | 22706224, 10.1097/IGC.0b013e318259d8da | The present study has focused on the identification of the differences between expression patterns of kinin-dependent genes in endometrial cancer with the use of real-time quantitative reverse transcription polymerase chain reaction and oligonucleotide microarray. | Aged, Carcinoma, Endometrioid, Endometrial Neoplasms, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Middle Aged, Oligonucleotide Array Sequence Analysis, RNA, Messenger, Real-Time Polymerase Chain Reaction, Receptor, Bradykinin B1, Receptor, Bradykinin B2, Reverse Transcriptase Polymerase Chain Reaction | null |
22,706,225 | 2012-11-08 | 2018-10-30 | 1525-1438 | International journal of gynecological cancer : official journal of the International Gynecological Cancer Society | Prevalence of human papillomavirus genotypes in cervical cancer. | Chinchai Teeraporn, Chansaenroj Jira, Swangvaree Sukumarn, Junyangdikul Pairoj, Poovorawan Yong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706225, 10.1097/IGC.0b013e318259d904 | Cervical cancer is the second most common female genital cancer worldwide. There is strong epidemiological and molecular evidence indicating that human papillomavirus (HPV) infection is a necessary event in the development of cervical intraepithelial lesion and subsequent invasive carcinoma. The aim of this study was to investigate the HPV genotype distribution and prevalence in cervical cancer of Thai women. | Adult, Aged, Aged, 80 and over, Alphapapillomavirus, Female, Genotyping Techniques, Humans, Middle Aged, Papillomavirus Infections, Thailand, Uterine Cervical Neoplasms | null |
22,706,226 | 2012-11-08 | 2018-10-30 | 1525-1438 | International journal of gynecological cancer : official journal of the International Gynecological Cancer Society | Could different follow-up modalities play a role in the diagnosis of asymptomatic endometrial cancer relapses?: an Italian multicentric retrospective analysis. | Carrara Luisa, Gadducci Angiolo, Landoni Fabio, Maggino Tiziano, Scambia Giovanni, Galletto Luciano, Lissoni Andrea Alberto, Fuso Luca, Zola Paolo, Sartori Enrico | eng | null | Comparative Study, Journal Article, Multicenter Study | null | IM | 22706226, 10.1097/IGC.0b013e31825ad3ee | To determine current practice and to assess the value of routine follow-up procedures for endometrial cancer surveillance. To discuss whether such procedures are feasible and effective to identify asymptomatic recurrences and describe the pattern of relapse detected by procedures. | Adult, Aged, Aged, 80 and over, Carcinoma, Endometrial Neoplasms, Female, Humans, Italy, Middle Aged, Neoplasm Recurrence, Local, Retrospective Studies | null |
22,706,223 | 2012-11-08 | 2018-10-30 | 1525-1438 | International journal of gynecological cancer : official journal of the International Gynecological Cancer Society | The role of lymphadenectomy in node-positive epithelial ovarian cancer. | Pereira Augusto, Pérez-Medina Tirso, Magrina Javier F, Magtibay Paul M, Millan Isabel, Iglesias Enrique | eng | null | Journal Article | null | IM | 22706223, 10.1097/IGC.0b013e318257b958 | To evaluate the therapeutic role of pelvic and aortic lymphadenectomy in patients with epithelial ovarian cancer (EOC) and positive nodes (stages IIIC and IV). | Adult, Aged, Aged, 80 and over, Aorta, Female, Humans, Lymph Node Excision, Lymph Nodes, Middle Aged, Neoplasms, Glandular and Epithelial, Ovarian Neoplasms, Pelvis, Retrospective Studies, United States, Young Adult | null |
22,706,229 | 2012-10-10 | 2012-06-18 | 0792-5077 | Drug metabolism and drug interactions | Drug metabolism and drug interactions and the European Society of Pharmacogenomics and Theranostics. | Siest Gérard | eng | null | Editorial, Introductory Journal Article | null | IM | 22706229, 10.1515/dmdi-2012-0009, /j/dmdi.2012.27.issue-2/dmdi-2012-0009/dmdi-2012-0009.xml | null | Drug Industry, Drug Interactions, Europe, Humans, Periodicals as Topic, Pharmacogenetics, Societies, Medical | null |
22,706,230 | 2012-10-10 | 2019-03-21 | 0792-5077 | Drug metabolism and drug interactions | Human cytochrome P450 4F3: structure, functions, and prospects. | Corcos Laurent, Lucas Danièle, Le Jossic-Corcos Catherine, Dréano Yvonne, Simon Brigitte, Plée-Gautier Emmanuelle, Amet Yolande, Salaün Jean-Pierre | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Fatty Acids, RNA, Messenger, Xenobiotics, Cytochrome P-450 Enzyme System, Cytochrome P450 Family 4, CYP4F3 protein, human | IM | 22706230, 10.1515/dmdi-2011-0037, /j/dmdi.2012.27.issue-2/dmdi-2011-0037/dmdi-2011-0037.xml | Cytochrome P450 4F3 (CYP4F3), originally identified as one of the leukotriene B4 ω-hydroxylases, belongs to a CYP gene family that comprises several members, which participate in the metabolism of various endobiotics, as well as some xenobiotics. The CYP4F gene family is clustered in a 0.5-Mb stretch of genomic DNA on the p13 region of chromosome 19. Apart from the ω-hydroxylation of leukotriene B4 and prostaglandins, CYP4F3 is the main catalyst in the oxidation of fatty acid epoxides. CYP4F3 expression results from the synthesis of two distinct enzymes, CYP4F3A and CYP4F3B, which originate from the alternative splicing of a single pre-mRNA precursor molecule. Remarkably, the selection of either isoform is part of a tissue-specific control through which CYP3F3A is mostly expressed in leukocytes and CYP4F3B mostly in the liver. Recently, CYP4F3 single nucleotide polymorphisms have been incriminated in the onset of pathologies, including celiac or Crohn's diseases. Although much has been discovered in the regulation and function of CYP4F2, the closest CYP4F subfamily member, analyses of CYP4F3 enzymes lag somewhat behind in the field of our knowledge. In this short review, emphasis will be placed on the regulation and the functional roles of human CYP4F3. | Alternative Splicing, Amino Acid Sequence, Animals, Cytochrome P-450 Enzyme System, Cytochrome P450 Family 4, Fatty Acids, Gene Expression Regulation, Enzymologic, Humans, Lipid Metabolism, Molecular Sequence Data, RNA, Messenger, Xenobiotics | null |
22,706,231 | 2012-10-10 | 2016-11-25 | 0792-5077 | Drug metabolism and drug interactions | CYP2A6: genetics, structure, regulation, and function. | Raunio Hannu, Rahnasto-Rilla Minna | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Coumarins, Enzyme Inhibitors, Nicotinic Agonists, Nicotine, coumarin, Aryl Hydrocarbon Hydroxylases, CYP2A6 protein, human, Cytochrome P-450 CYP2A6 | IM | 22706231, 10.1515/dmdi-2012-0001, /j/dmdi.2012.27.issue-2/dmdi-2012-0001/dmdi-2012-0001.xml | The human CYP2A gene subfamily consists of three members, CYP2A6, CYP2A7, and CYP2A13. The CYP2A6 gene is highly polymorphic with approximately 40 annotated allelic variants. Individuals homozygous for some of these alleles have a total lack of CYP2A6 activity. The CYP2A6 protein is most abundant in liver and is expressed, although at much lower levels, in some other tissues, especially nasal mucosa. CYP2A6 differs from other human liver CYP forms in that it participates in the metabolism of very few currently used drugs. The two most relevant substrates for CYP2A6 are coumarin and nicotine. Coumarin is the marker substance for determining CYP2A6 activity both in vitro and in vivo. Approximately 80% of a nicotine dose is eliminated by CYP2A6, and there is a clear link between CYP2A6 genotypes, smoking behavior, and lung cancer risk. | Animals, Aryl Hydrocarbon Hydroxylases, Coumarins, Cytochrome P-450 CYP2A6, Enzyme Inhibitors, Gene Expression Regulation, Enzymologic, Humans, Mice, Models, Molecular, Multigene Family, Neoplasms, Nicotine, Nicotinic Agonists, Polymorphism, Genetic, Smoking, Substrate Specificity | null |
22,706,232 | 2012-10-10 | 2015-11-19 | 0792-5077 | Drug metabolism and drug interactions | The role of biomarkers in the development of novel cancer therapies. | Jungić Saša, Tubić Biljana, Skrepnik Tijana | eng | null | Journal Article, Review | Biomarkers | IM | 22706232, 10.1515/dmdi-2011-0036, /j/dmdi.2012.27.issue-2/dmdi-2011-0036/dmdi-2011-0036.xml | The etiology of diverse patient responses to a given pharmaceutical treatment has eluded science for decades. Only during the last 10-15 years has our understanding of the interplay between genetics and pharmaceuticals advanced to the point that personalized medicine may optimize therapies for each individual patient. The primary goals of personalized medicine are identifying individuals at risk of developing disease to better prevent disease in the healthy population, accurately monitoring each patient's response to therapy and predicting recurrence in order to pre-empt it. This review gives an explanation of biomarkers and addresses their role in the diagnosis and surveillance of various cancers. It also addresses the challenges of developing novel therapies utilizing newly discovered biomarkers. | Animals, Biomarkers, Humans, Legislation, Medical, Neoplasms, Precision Medicine, Predictive Value of Tests, Prognosis, Reproducibility of Results, Research | null |
22,706,233 | 2012-10-10 | 2013-11-21 | 0792-5077 | Drug metabolism and drug interactions | Molecular mechanisms underlying statin effects on genes involved in the reverse cholesterol transport. | Cerda Alvaro, Hirata Mario Hiroyuki, Hirata Rosario Dominguez Crespo | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Anticholesteremic Agents, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Lipoproteins, HDL, MicroRNAs, Cholesterol | IM | 22706233, 10.1515/dmdi-2012-0007, /j/dmdi.2012.27.issue-2/dmdi-2012-0007/dmdi-2012-0007.xml | Many clinical trials and data from scientific investigations have suggested the effects of statins on high-density lipoprotein (HDL) metabolism, besides their actions in reducing low-density lipoprotein (LDL) cholesterol. These actions have been proposed as important anti-atherogenic properties that contribute to the additional reduction of risk for cardiovascular diseases. The regulation of genes involved in the reverse cholesterol transport (RCT) is very complex and the modulation exerted by statin treatment is poorly understood. In this review, we discuss the molecular mechanisms underlying the modulation of genes controlling the RCT with special emphasis on the reported tissue-specific effects of statins. The statin modulation of genes participating in the different stages of RCT (cholesterol efflux from peripheral tissues, HDL metabolism in the plasma and internalization by the liver) has been summarized. Recent reports on novel mechanisms of regulation by microRNAs are also discussed. | Animals, Anticholesteremic Agents, Cholesterol, Gene Expression Regulation, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Hypercholesterolemia, Lipoproteins, HDL, MicroRNAs, Protein Processing, Post-Translational | null |
22,706,238 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Laboratory maternal-fetal medicine: challenges and perspectives. | Plebani Mario | eng | null | Editorial, Introductory Journal Article | null | IM | 22706238, 10.1515/cclm-2012-0297, /j/cclm.2012.50.issue-6/cclm-2012-0297/cclm-2012-0297.xml | null | Biomedical Research, Female, Fetus, Humans, Laboratories, Mothers, Pregnancy, Pregnancy Complications | null |
22,706,235 | 2012-10-10 | 2018-12-01 | 0792-5077 | Drug metabolism and drug interactions | Need for pharmacogenomic information also for generic medications: recommendation of the European Society of Pharmacogenomics and Theranostics (ESPT). | Seist Gérard | eng | null | Journal Article | Drugs, Generic, Platelet Aggregation Inhibitors, Clopidogrel, Aryl Hydrocarbon Hydroxylases, CYP2C19 protein, human, Cytochrome P-450 CYP2C19, Ticlopidine | IM | 22706235, 10.1515/dmdi-2012-0010, /j/dmdi.2012.27.issue-2/dmdi-2012-0010/dmdi-2012-0010.xml | null | Aryl Hydrocarbon Hydroxylases, Clopidogrel, Consensus, Cytochrome P-450 CYP2C19, Drug Labeling, Drugs, Generic, Europe, France, Humans, Pharmacogenetics, Platelet Aggregation Inhibitors, Societies, Medical, Ticlopidine | null |
22,706,234 | 2012-10-10 | 2013-11-21 | 0792-5077 | Drug metabolism and drug interactions | Local concentration of systemic amoxicillin and metronidazole in healthy and inflamed gingiva: a comparative in vivo study. | Amid Reza, Tabeie Mohammad Bagher, Kadkhodazadeh Mahdi, Mehdizadeh Amir Reza, Youssefi Navid | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Drug Combinations, Metronidazole, Amoxicillin | IM | 22706234, 10.1515/dmdi-2012-0003, /j/dmdi.2012.27.issue-2/dmdi-2012-0003/dmdi-2012-0003.xml | One of the most recommended methods of systemic antibiotic administration in periodontics is the combination of amoxicillin and metronidazole, which has great inhibitory effect on periodontal pathogens. The aim of this study is to determine the local concentrations of these drugs in gingiva and compare its distribution in healthy and inflamed tissues. | Administration, Oral, Amoxicillin, Anti-Bacterial Agents, Chromatography, High Pressure Liquid, Drug Combinations, Gingiva, Gingivitis, Humans, Metronidazole, Periodontal Pocket, Periodontitis | null |
22,706,239 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Candidate biochemical markers for screening of pre-eclampsia in early pregnancy. | Forest Jean-Claude, Charland Marc, Massé Jacques, Bujold Emmanuel, Rousseau François, Lafond Julie, Giguère Yves | eng | null | Journal Article, Review | Biomarkers | IM | 22706239, 10.1515/cclm.2011.820, /j/cclm.2012.50.issue-6/cclm.2011.820/cclm.2011.820.xml | Pre-eclampsia (PE) and other hypertensive disorders of pregnancy (HDP) are a leading cause of adverse outcomes. Their pathophysiology remains elusive, hampering the development of efficient prevention. The onset of HDP and PE and the severity of their clinical manifestations are heterogeneous. The advent of preventive measures, such as low-dose aspirin that targets high-risk women, emphasizes the need of better prediction. Until recently, only environmental information and maternal risk factors were considered, with equivocal predictive value. No validated screening procedures were available to identify at-risk women despite the emergence of Doppler ultrasonography parameters for the uterine artery (e.g., pulsatility index and bilateral notching) and pathophysiological biochemical markers (e.g., angiogenesis, inflammation, and endothelial dysfunction). Owing to its heterogeneity and lack of specific, sensitive markers among those studied so far (>200), PE is unlikely to be detected early by a single predictive parameter. Systematic reviews have concluded that no single test fulfilling World Health Organization criteria for biomarker selection can diagnose/predict a disease. However, by combining antenatal risk factors, clinical parameters, as well as biophysical and biochemical markers into multivariate algorithms, the risk of PE can be estimated with performance levels that could reach clinical utility. Performance characteristics of selected algorithms will be presented and discussed with respect to transferability to different geographic and healthcare environments. | Biomarkers, Embryo Implantation, Female, Humans, Pre-Eclampsia, Pregnancy, Risk Factors | null |
22,706,240 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Are biological markers relevant for the diagnosis and the prognosis of preterm premature rupture of membranes (PPROM)? | Marcellin Louis, Goffinet François | eng | null | Journal Article, Review | Biomarkers | IM | 22706240, 10.1515/cclm-2011-1850, /j/cclm.2012.50.issue-6/cclm-2011-1850/cclm-2011-1850.xml | The preterm premature rupture of membranes (PPROM) is a common condition in pregnant women and is associated with significant maternal and perinatal morbidity. Most of the time, the diagnosis is done during physical examination. However, in 10%-20% of equivocal cases, biological markers are needed to confirm the diagnosis, especially when leakage of fluid is low or intermittent. In these cases, a quick and reliable diagnosis is necessary for applying the appropriate measures to reduce perinatal complications. The prognosis in PPROM is linked to maternal inflammatory markers that might predict perinatal infection, and therefore be helpful to decide the timing of the delivery. Nevertheless, further research is needed to identify robust biological markers for the diagnosis of PPROM in equivocal cases and for the prognosis. | Biomarkers, Extraembryonic Membranes, Female, Fetal Membranes, Premature Rupture, Humans, Pregnancy, Prognosis | null |
22,706,241 | 2012-10-16 | 2017-12-13 | 1437-4331 | Clinical chemistry and laboratory medicine | Study of FTMT and ABCA4 genes in a patient affected by age-related macular degeneration: identification and analysis of new mutations. | Stenirri Stefania, Santambrogio Paolo, Setaccioli Marco, Erba Benedetta Gaia, Pia Manitto Maria, Rovida Ermanna, Ferrari Maurizio, Levi Sonia, Cremonesi Laura | eng | null | Case Reports, Journal Article, Research Support, Non-U.S. Gov't | ABCA4 protein, human, ATP-Binding Cassette Transporters, Mitochondrial Proteins, mitochondrial ferritin, human, Ferritins | IM | 22706241, 10.1515/cclm-2011-0854, /j/cclm.2012.50.issue-6/cclm-2011-0854/cclm-2011-0854.xml | Age-related macular degeneration (AMD) is a multifactorial disease for which an involvement of alterations in the retinal ABC transporter gene (ABCA4) is still debated. Oxidative stress in retinal pigment epithelial cells has been postulated to contribute to the pathogenesis of the disease. Mitochondrial ferritin (FtMt), an iron-sequestering protein, is expressed in cell types characterized by high metabolic activity and oxygen consumption, including human retina, suggesting a role in protecting mitochondria from iron-dependent oxidative damage. Based on these findings we wanted to investigate whether mutations in this gene could be found in AMD patients. | ATP-Binding Cassette Transporters, Aged, 80 and over, Base Sequence, Cohort Studies, DNA Mutational Analysis, Female, Ferritins, Humans, Macular Degeneration, Mitochondrial Proteins, Models, Molecular, Mutation, Protein Structure, Quaternary, Protein Structure, Tertiary | null |
22,706,242 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Rapid detection of CFH (p.Y402H) and ARMS2 (p.A69S) polymorphisms in age-related macular degeneration using high-resolution melting analysis. | Mello Enrica, Falsini Benedetto, Zuppi Cecilia, Giardina Bruno, Concolino Paola, Capoluongo Ettore | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Complement Factor H | IM | 22706242, 10.1515/cclm-2011-0859, /j/cclm.2012.50.issue-6/cclm-2011-0859/cclm-2011-0859.xml | Age-related macular degeneration (AMD) is a complex disorder causing irreversible central vision loss. Complement Factor H (CFH) and age-related maculopathy susceptibility 2 (ARMS2) are now widely accepted as important AMD susceptibility genes. In particular, two specific variants, CFH p.Y402H and ARMS2 p.A69S, have been reported as strongly AMD associated. In order to perform the genetic screening of these single nucleotide polymorphisms (SNPs), we describe a high resolution melting analysis (HRM) as a rapid closed tube mutation scanning assay. | Complement Factor H, Genotyping Techniques, Humans, Macular Degeneration, Nucleic Acid Denaturation, Polymorphism, Single Nucleotide, Time Factors, Transition Temperature | null |
22,706,244 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Volume, rates, source and types of add-on pathology test requests across five hospitals. | Vecellio Elia, Georgiou Andrew, Toouli George, Westbrook Johanna I | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706244, 10.1515/cclm-2011-0756, /j/cclm.2012.50.issue-6/cclm-2011-0756/cclm-2011-0756.xml | Add-on test requests, where a clinician requests further test assays on an existing specimen, contribute disproportionately to pathology service workload. However, little research has quantified the volume, rates, source or types of add-on tests. This study provides a descriptive analysis of add-on testing within a pathology service serving five hospitals. | Clinical Chemistry Tests, Hematologic Tests, Hospitals, Humans, Pathology Department, Hospital, Specimen Handling, Time Factors | null |
22,706,243 | 2012-10-16 | 2016-11-25 | 1437-4331 | Clinical chemistry and laboratory medicine | Unlabeled-probe high-resolution melting to detect KRAS codon 12 and 13 mutations in pancreatic adenocarcinoma tissues. | Guo Wei, Zhang Chunyan, Wu Jiong, Song Binbin, Wang Beili, Zhou Yan, Zhou Jiaye, Shen Minna, Zhang Chen, Zhang Xinju, Guan Ming, Pan Baishen | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Codon, DNA Probes, KRAS protein, human, Proto-Oncogene Proteins, DNA, Proto-Oncogene Proteins p21(ras), ras Proteins | IM | 22706243, 10.1515/cclm-2011-0601, /j/cclm.2012.50.issue-6/cclm-2011-0601/cclm-2011-0601.xml | The aim of our study was to establish an unlabeled-probe high-resolution melting (HRM) approach to the detection of Kirsten RAS (KRAS) codon 12 and 13 mutations in pancreatic adenocarcinoma (PA) tissues as a novel and effective diagnostic technique. | Adenocarcinoma, Adult, Aged, Aged, 80 and over, Codon, DNA, DNA Mutational Analysis, DNA Probes, Female, Humans, Male, Middle Aged, Nucleic Acid Denaturation, Pancreatic Neoplasms, Proto-Oncogene Proteins, Proto-Oncogene Proteins p21(ras), Reproducibility of Results, Transition Temperature, ras Proteins | null |
22,706,245 | 2012-10-16 | 2017-11-16 | 1437-4331 | Clinical chemistry and laboratory medicine | Schizophrenia is associated with increased levels of serum Fas and FasL. | Djordjević Vladimir V, Ristić Tatjana, Lazarević Dušan, Cosić Vladan, Vlahović Predrag, Djordjević Vidosava B | eng | null | Journal Article, Research Support, Non-U.S. Gov't | FAS protein, human, Fas Ligand Protein, fas Receptor, Caspase 3 | IM | 22706245, 10.1515/cclm-2011-0684, /j/cclm.2012.50.issue-6/cclm-2011-0684/cclm-2011-0684.xml | A growing body of evidence suggests that the apoptotic process is dysregulated in schizophrenia. However, only a few studies have evaluated apoptotic markers in vivo in patients or their cell cultures. | Adult, Case-Control Studies, Caspase 3, Fas Ligand Protein, Female, Humans, Lymphocytes, Male, ROC Curve, Schizophrenia, fas Receptor | null |
22,706,246 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Comparison between capillary, venous and arterial levels of protein S100B in patients with severe brain pathology. | Astrand Ramona, Romner Bertil, Reinstrup Peter, Friis-Hansen Lennart, Undén Johan | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Nerve Growth Factors, S100 Calcium Binding Protein beta Subunit, S100 Proteins, S100B protein, human | IM | 22706246, 10.1515/cclm-2011-0639, /j/cclm.2012.50.issue-6/cclm-2011-0639/cclm-2011-0639.xml | Protein S100B is soon in clinical use as a sensitive marker after mild traumatic head injury in adults. Initial studies of S100B in pediatric head injury have shown promising results. Venous sampling can be challenging in children and capillary samples are often a preferred option. The aim of the study was to investigate the relation between capillary, venous and arterial measurements of protein S100B, primarily by determining whether capillary S100B differ from venous and if capillary S100B can predict venous S100B levels, and secondarily, if arterial S100B samples can substitute venous samples in severely brain-injured patients. | Adolescent, Adult, Aged, Arteries, Blood Chemical Analysis, Brain Injuries, Capillaries, Female, Humans, Male, Middle Aged, Nerve Growth Factors, S100 Calcium Binding Protein beta Subunit, S100 Proteins, Veins, Young Adult | null |
22,706,248 | 2012-10-16 | 2017-11-16 | 1437-4331 | Clinical chemistry and laboratory medicine | Lack of consistency between two commercial ELISAs and against an in-house ELISA for the detection of CD36 in human plasma. | Lykkeboe Simon, Larsen Anne Lone, Handberg Aase | eng | null | Journal Article | CD36 Antigens | IM | 22706248, 10.1515/cclm-2011-0950, /j/cclm.2012.50.issue-6/cclm-2011-0950/cclm-2011-0950.xml | CD36 is a widely expressed cell surface receptor, that among other ligands binds lipoproteins, and its function has been implicated in many of the complications belonging to the metabolic syndrome. We have previously identified a circulating form of CD36 and established an in-house ELISA assay for measurement of CD36 in plasma. Plasma CD36 was elevated in insulin resistant obese and diabetic patients, and in patients with unstable atherosclerotic plaques. The objective of this study was to compare two new commercial CD36 ELISA assays and our in-house ELISA assay. | Blood Chemical Analysis, CD36 Antigens, Enzyme-Linked Immunosorbent Assay, Humans, Reference Standards, Reproducibility of Results | null |
22,706,247 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Changes of serum adiponectin and soluble intercellular adhesion molecule-1 concentrations after smoking cessation. | Tsai Jaw-Shiun, Guo Fei-Ran, Chen Su-Chiu, Lue Bee-Horng, Lee Long-Teng, Huang Kuo-Chin, Chen Chin-Ying, Hung Shou-Hung, Chuang Lee-Ming, Chen Ching-Yu | eng | null | Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Adiponectin, Intercellular Adhesion Molecule-1 | IM | 22706247, 10.1515/cclm-2011-0852, /j/cclm.2012.50.issue-6/cclm-2011-0852/cclm-2011-0852.xml | This study aims to investigate the effect of increased serum adiponectin concentration during smoking cessation on soluble intercellular adhesion molecule-1 (sICAM-1) concentration. | Adiponectin, Adult, Aged, Female, Humans, Intercellular Adhesion Molecule-1, Male, Middle Aged, Regression Analysis, Smoking Cessation, Solubility | null |
22,706,249 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Comparison of a commercial urinary neopterin radioimmunoassay with high performance liquid chromatography. | Hammerer-Lercher Angelika, Moser Christina, Leichtfried Veronika, Schobersberger Wolfgang, Griesmacher Andrea, Fuchs Dietmar | eng | null | Comparative Study, Journal Article | Neopterin | IM | 22706249, 10.1515/cclm-2011-0861, /j/cclm.2012.50.issue-6/cclm-2011-0861/cclm-2011-0861.xml | The determination of urinary neopterin using high performance liquid chromatography (HPLC) has routinely been performed in Innsbruck since the middle of the 1980s. The aim was to compare the HPLC method with a commercially available radioimmunoassay. | Adult, Chromatography, High Pressure Liquid, Female, Humans, Male, Neopterin, Radioimmunoassay, Urinalysis | null |
22,706,250 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Evaluation of Prevent ID and Quantum Blue rapid tests for fecal calprotectin. | Hessels Jan, Douw Geke, Yildirim Duygu D, Meerman Gerrit, van Herwaarden Margot A, van den Bergh Frank A J T M | eng | null | Journal Article | Leukocyte L1 Antigen Complex | IM | 22706250, 10.1515/cclm-2011-0855, /j/cclm.2012.50.issue-6/cclm-2011-0855/cclm-2011-0855.xml | Tests for fecal calprotectin are usually either enzyme-linked immunosorbent assays (ELISA) or a time-resolved fluorimetric immunoassay (TRFIA). These time-consuming tests are performed only once every 1 or 2 weeks. Before the results of the tests are known most patients have already undergone colonoscopy. A rapid test, performed on outpatients, could minimize the number of necessary colonoscopies. To establish optimal cut-off values minimizing the necessity for colonoscopies, we compared two commercially available rapid tests with a quantitative TRFIA. | Clinical Chemistry Tests, Colonoscopy, Feces, Gastroenterology, Humans, Leukocyte L1 Antigen Complex, Referral and Consultation, Time Factors | null |
22,706,251 | 2012-10-16 | 2019-12-10 | 1437-4331 | Clinical chemistry and laboratory medicine | Analytical performance evaluation of four cartridge-type blood gas analyzers. | De Koninck Anne-Sophie, De Decker Koen, Van Bocxlaer Jan, Meeus Peter, Van Hoovels Lieve | eng | null | Evaluation Study, Journal Article | null | IM | 22706251, 10.1515/cclm-2011-0685, /j/cclm.2012.50.issue-6/cclm-2011-0685/cclm-2011-0685.xml | The immediate impact of blood gas test results on patient care favors the use of blood gas analyzers as point-of-care-testing (POCT) devices. We performed an analytical performance evaluation of four cartridge-type blood gas analyzers for the determination of pH, partial carbon dioxide pressure (pCO2), partial oxygen pressure (pO2), ionized calcium (iCa2+), potassium (K+), glucose, lactate and total hemoglobin (tHb), in comparison with a traditional blood gas analyzer. | Blood Gas Analysis, Humans, Point-of-Care Systems, Sensitivity and Specificity | null |
22,706,252 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Serum free light chains and oligoclonal bands in patients with multiple myeloma and autologous stem cell transplantation. | Alejandre Mariel Emilce, Pavlovsky Miguel Arturo, Remaggi Guillermina, Corrado Claudia, Fernandez Isolda, Milone Gustavo, Pavlovsky Astrid, Madalena Leticia, Pandolfo Marcela, Facio María Laura, Bresciani Pablo, Pavlovsky Santiago, Pizzolato Marco A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Immunoglobulin Light Chains, Oligoclonal Bands | IM | 22706252, 10.1515/cclm-2011-0544, /j/cclm.2012.50.issue-6/cclm-2011-0544/cclm-2011-0544.xml | To establish stringent complete remission (SCR) in patients with multiple myeloma (MM), it is currently recommended to obtain a normal serum free light chains (sFLC) ratio. The appearance of serum oligoclonal bands (OB) after autologous stem cell transplantation (ASCT) is considered a favorable prognostic factor. The objective of this study was to examine sFLC for assessing SCR in patients with MM, and ASCT with OB. We also examined how capillary electrophoresis (CE) compares with agarose gel electrophoresis (Aga) in identifying oligoclonal bands. | Adult, Aged, Electrophoresis, Capillary, Female, Humans, Immunoglobulin Light Chains, Male, Middle Aged, Multiple Myeloma, Oligoclonal Bands, Stem Cell Transplantation, Transplantation, Autologous | null |
22,706,253 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Homocysteine as a determinant of left ventricular ejection fraction in patients with diabetes. | Badiou Stephanie, Dupuy Anne-Marie, Jaussent Isabelle, Sultan Ariane, Mariano-Goulart Denis, Cristol Jean-Paul, Avignon Antoine | eng | null | Journal Article | Biomarkers, Homocysteine | IM | 22706253, 10.1515/cclm-2011-0851, /j/cclm.2012.50.issue-6/cclm-2011-0851/cclm-2011-0851.xml | A link between homocysteine (Hcy) and left ventricular ejection fraction (LVEF) emerged from recent studies but was yet not explored specifically in diabetic patients. This study aimed to assess the relationship between LVEF and Hcy in a cohort of adults with diabetes. | Adult, Aged, Aged, 80 and over, Analysis of Variance, Biomarkers, Cohort Studies, Diabetes Mellitus, Female, Homocysteine, Humans, Male, Middle Aged, Stroke Volume, Ventricular Function, Left | null |
22,706,256 | 2012-10-16 | 2018-12-01 | 1437-4331 | Clinical chemistry and laboratory medicine | Effects of endothelin-1 on endothelial progenitor cell function. | Diehl Kyle J, Weil Brian R, Westby Christian M, MacEneaney Owen J, Kushner Erich J, Greiner Jared J, DeSouza Christopher A | eng | HL076434 (NHLBI NIH HHS, United States); HL077450 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Endothelin-1, Vascular Endothelial Growth Factor A | IM | 22706256, 10.1515/cclm-2011-0670, /j/cclm.2012.50.issue-6/cclm-2011-0670/cclm-2011-0670.xml | Circulating endothelial progenitor cells (EPCs) contribute to vascular endothelial repair. Endothelin (ET)-1 is associated with endothelial damage and atherogenesis. The experimental aim of this study was to determine, in vitro, the effects of ET-1 on the ability of EPCs to form colonies, migrate, release angiogenic growth factors and resist apoptosis. | Adult, Apoptosis, Cell Movement, Endothelin-1, Endothelium, Vascular, Humans, Stem Cells, Vascular Endothelial Growth Factor A, Young Adult | null |
22,706,254 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Plasma total homocysteine is a determinant of carotid intima-media thickness and circulating endothelial progenitor cells in patients with newly diagnosed hypertension. | Bogdanski Paweł, Miller-Kasprzak Ewa, Pupek-Musialik Danuta, Jablecka Anna, Lacinski Mariusz, Jagodzinski Piotr P, Jakubowski Hieronim | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antioxidants, Homocysteine | IM | 22706254, 10.1515/cclm-2011-0856, /j/cclm.2012.50.issue-6/cclm-2011-0856/cclm-2011-0856.xml | Accumulating evidence suggests that elevated plasma homocysteine (Hcy), prevalent in hypertensive patients, affects oxidant/antioxidant balance of the body, and is linked to the development of atherosclerosis, inflammation, and endothelium injury. Our objective was to examine a hypothesis that Hcy is a predictor of total antioxidant status (TAS) and endothelial progenitor cells (EPCs), important in the repair of injured endothelium, in hypertensive patients. | Adult, Antioxidants, Carotid Intima-Media Thickness, Endothelium, Homocysteine, Humans, Hypertension, Oxidative Stress, Stem Cells | null |
22,706,255 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Serum MMP-9 and TIMP-1 concentrations and MMP-9 activity during surgery-induced inflammation in humans. | Helmersson-Karlqvist Johanna, Akerfeldt Torbjörn, Gunningberg Lena, Swenne Christine Leo, Larsson Anders | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Tissue Inhibitor of Metalloproteinase-1, Matrix Metalloproteinase 9 | IM | 22706255, 10.1515/cclm-2011-0234, /j/cclm.2012.50.issue-6/cclm-2011-0234/cclm-2011-0234.xml | Matrix metalloproteinase 9 (MMP-9) and the endogenous inhibitor to MMP-9, tissue inhibitor of metalloproteinase 1 (TIMP-1), have important roles in tissue remodelling and are implicated in a number of diseases related to inflammation. The time course in activation and formation of MMPs and TIMPs during an inflammatory reaction is not fully known. This study investigates MMP-9 and TIMP-1 concentrations and MMP-9 activity at different time points after major surgery when a state of noticeable inflammation is expected. | Aged, Aged, 80 and over, Coronary Artery Bypass, Female, Humans, Inflammation, Linear Models, Male, Matrix Metalloproteinase 9, Middle Aged, Orthopedics, Postoperative Complications, Tissue Inhibitor of Metalloproteinase-1 | null |
22,706,257 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Investigation of cystatin C and cystatin C based estimated glomerular filtration rate in pregnant patients with heart failure. | Xu Mengyan, Guo Zhidong, Wang Qiqi, Jin Jianmei, Wu Tao, Qiu Yuangang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cystatin C, Creatinine | IM | 22706257, 10.1515/cclm-2011-0740, /j/cclm.2012.50.issue-6/cclm-2011-0740/cclm-2011-0740.xml | Impaired cardiac function leads to impaired renal function. We assessed renal function in pregnant patients with heart failure. | Adult, Creatinine, Cystatin C, Female, Glomerular Filtration Rate, Heart Failure, Humans, Kidney, Pregnancy, Pregnancy Complications | null |
22,706,258 | 2012-10-16 | 2018-12-01 | 1437-4331 | Clinical chemistry and laboratory medicine | Comments on a performance evaluation of cartridge-type blood gas analyzers. | D'Orazio Paul | eng | null | Letter, Comment | null | IM | 22706258, 10.1515/cclm-2012-0216, /j/cclm.2012.50.issue-6/cclm-2012-0216/cclm-2012-0216.xml | null | Blood Gas Analysis, Humans | null |
22,706,260 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Carryover can be a cause of false-positive results with the Beckman AccuTnI assay. | Dimeski Goce, Jones Brock, Brown Nigel | eng | null | Letter | Troponin I | IM | 22706260, 10.1515/cclm-2011-8838, /j/cclm.2012.50.issue-6/cclm-2011-8838/cclm-2011-8838.xml | null | Blood Chemical Analysis, Blood Specimen Collection, False Positive Reactions, Humans, Troponin I | null |
22,706,261 | 2012-10-16 | 2018-08-13 | 1437-4331 | Clinical chemistry and laboratory medicine | Method comparison of a 25-hydroxy vitamin D enzyme immunoassay to liquid chromatography tandem mass spectroscopy. | Kelley James M, Melanson Stacy E F, Snyder Marion L, Cremers Serge, Jarolim Petr | eng | null | Comparative Study, Letter | Vitamin D, 25-hydroxyvitamin D | IM | 22706261, 10.1515/cclm-2011-0703, /j/cclm.2012.50.issue-6/cclm-2011-0703/cclm-2011-0703.xml | null | Chromatography, Liquid, Humans, Immunoenzyme Techniques, Tandem Mass Spectrometry, Vitamin D | null |
22,706,263 | 2012-10-16 | 2016-10-18 | 1437-4331 | Clinical chemistry and laboratory medicine | Sharing our experience in improving postgraduate clinical biochemistry training with laboratory practice articles. | Uysal Sezer, Coker Canan, Onvural Banu | eng | null | Letter | null | IM | 22706263, 10.1515/cclm-2011-0944, /j/cclm.2012.50.issue-6/cclm-2011-0944/cclm-2011-0944.xml | null | Biochemistry, Chemistry, Clinical, Medical Laboratory Personnel | null |
22,706,262 | 2012-10-16 | 2019-12-10 | 1437-4331 | Clinical chemistry and laboratory medicine | Bone marrow plasma cell separation - validation of separation algorithm. | Buresova Ivana, Cumova Jana, Kovarova Lucie, Stossova Jana, Dementyeva Elena, Kryukov Fedor, Sevcikova Sabina, Svachova Hana, Hajek Roman | eng | null | Letter, Research Support, Non-U.S. Gov't, Validation Study | null | IM | 22706262, 10.1515/cclm-2012-8837, /j/cclm.2012.50.issue-6/cclm-2012-8837/cclm-2012-8837.xml | null | Adult, Aged, Aged, 80 and over, Algorithms, Bone Marrow Cells, Cell Separation, Female, Humans, Male, Middle Aged, Plasma Cells | null |
22,706,264 | 2012-10-11 | 2012-06-18 | 1548-923X | International journal of nursing education scholarship | Creating a culture of evidence in nursing education using student portfolios. | Rossetti Jeanette, Oldenburg Nancy, Fisher Robertson Julie, Coyer Sharon M, Koren Mary E, Peters Bradley, Uhlken Connie, Musker Kathleen | eng | null | Journal Article | null | IM | 22706264, 10.1515/1548-923X.2415, /j/ijnes.2012.9.issue-1/1548-923X.2415/1548-923X.2415.xml | There has been a growing interest in the use of portfolios to assess nursing student progress in areas such as critical thinking, problem solving, and communication. The data obtained from student portfolios can be used to improve undergraduate nursing curricula; however in order for change to occur, closing the assessment feedback loop is essential. The portfolio program of one Midwestern school of nursing has successfully used portfolio evidence to effect undergraduate curricular change. This portfolio program, under the direction of the school's Portfolio Subcommittee, involves active participation by students, faculty and administration. Examples of curricular improvements based on portfolio findings included increasing rigor in course assignments, improving student self reflection, promoting students' ability to write in the discipline, and maintaining writing assignments in larger classes. | Curriculum, Education, Nursing, Baccalaureate, Educational Measurement, Faculty, Nursing, Humans, Nursing Education Research, Professional Staff Committees | null |
22,706,265 | 2013-07-29 | 2012-11-19 | 1878-1519 | Respiratory physiology & neurobiology | New insights into the many functions of carbonic anhydrase in fish gills. | Gilmour Kathleen M | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Isoenzymes, Carbonic Anhydrases | IM | 22706265, S1569-9048(12)00142-5, 10.1016/j.resp.2012.06.001 | Carbonic anhydrase (CA) is a zinc metalloenzyme that catalyzes the reversible reactions of carbon dioxide and water: CO(2) + H(2)O ↔ H(+) + HCO(3)(-). It has long been recognized that CA is abundant in the fish gill, with attention focused on the role of CA in catalyzing the hydration of CO(2) to provide H(+) and HCO(3)(-) for the branchial ion transport processes that underlie systemic ionic and acid-base regulation. Recent work has explored the diversity of CA isoforms in the fish gill. By linking these isoforms to different cell types in the gill, and by exploiting the diversity of fish species available for study, this work is increasing our understanding of the many roles that CA plays in the fish gill. In particular, recent work has revealed that fish utilize more than one model of CO(2) excretion, that to understand the role of CA and the gill in ionic regulation and acid-base balance means characterizing the transporter and CA complement of individual cell types, and that CA plays roles in branchial sensory mechanisms. The goal of this brief review is to summarize these new developments, while at the same time highlighting key areas in which further research is needed. | Acid-Base Equilibrium, Animals, Carbonic Anhydrases, Fishes, Gills, Isoenzymes | null |
22,706,266 | 2012-09-04 | 2021-10-21 | 1546-1726 | Nature neuroscience | Pattern and not magnitude of neural activity determines dendritic spine stability in awake mice. | Wyatt Ryan M, Tring Elaine, Trachtenberg Joshua T | eng | MH077972 (NIMH NIH HHS, United States); R01 EY016052 (NEI NIH HHS, United States); P50 MH077972 (NIMH NIH HHS, United States); EY016052 (NEI NIH HHS, United States); R01 EY023871 (NEI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Channelrhodopsins | IM | 22706266, nn.3134, 10.1038/nn.3134, PMC3386353, NIHMS377601, 17920014, 22179110, 20164928, 20376005, 15475951, 19617885, 17442243, 12850432, 6835395, 20869600, 15664179, 10862703, 21750548, 12801419, 19693029, 15457262, 19252494, 12490942, 16015331, 11086982, 19005470, 19396159 | The stability of dendritic spines in the neocortex is profoundly influenced by sensory experience, which determines the magnitude and pattern of neural firing. By optically manipulating the temporal structure of neural activity in vivo using channelrhodopsin-2 and repeatedly imaging dendritic spines along these stimulated neurons over a period of weeks, we show that the specific pattern, rather than the total amount of activity, determines spine stability in awake mice. | Action Potentials, Amygdala, Animals, Channelrhodopsins, Dendritic Spines, Mice, Mice, Transgenic, Neocortex, Neural Pathways, Sensory Receptor Cells, Wakefulness | null |
22,706,267 | 2012-09-04 | 2022-03-09 | 1546-1726 | Nature neuroscience | Distinct molecular pathways mediate glial activation and engulfment of axonal debris after axotomy. | Ziegenfuss Jennifer S, Doherty Johnna, Freeman Marc R | eng | R01 NS053538 (NINDS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | null | IM | 22706267, nn.3135, 10.1038/nn.3135, PMC4976689, NIHMS801291, 18172512, 9179599, 19707574, 19915564, 18432193, 17960134, 9602498, 9760700, 9635426, 11595183, 19369546, 11703939, 15042586, 2830320, 20126385, 11163267, 16772170, 3437074, 15457211, 16022592, 11703940, 17591687, 22426252, 17180163, 21606367, 10899007, 19782411, 8657152, 12765609, 1936965, 28306125, 12461569, 15744306, 10707082, 18351800, 15473839, 12134158, 16980964, 10441755, 11163239, 18567623, 17344397, 16772168, 11919635, 16772169 | Glial cells efficiently recognize and clear cellular debris after nervous system injury to maintain brain homeostasis, but pathways governing glial responses to neural injury remain poorly defined. We identify the Drosophila melanogaster guanine nucleotide exchange factor complex Crk/Mbc/dCed-12 and the small GTPase Rac1 as modulators of glial clearance of axonal debris. We found that Crk/Mbc/dCed-12 and Rac1 functioned in a non-redundant fashion with the Draper transmembrane receptor pathway: loss of either pathway fully suppressed clearance of axonal debris. Draper signaling was required early during glial responses, promoting glial activation, which included increased Draper and dCed-6 expression and extension of glial membranes to degenerating axons. In contrast, the Crk/Mbc/dCed-12 complex functioned at later phases, promoting glial phagocytosis of axonal debris. Our work identifies new components of the glial engulfment machinery and shows that glial activation, phagocytosis of axonal debris and termination of responses to injury are genetically separable events mediated by distinct signaling pathways. | Animals, Axons, Axotomy, Drosophila melanogaster, Gene Knockout Techniques, Neural Pathways, Neuroglia, Olfactory Receptor Neurons, Phagocytosis | null |
22,706,268 | 2012-09-04 | 2024-06-10 | 1546-1726 | Nature neuroscience | A mechanism for value-guided choice based on the excitation-inhibition balance in prefrontal cortex. | Jocham Gerhard, Hunt Laurence T, Near Jamie, Behrens Timothy E J | eng | 088312 (Wellcome Trust, United Kingdom); WT088312AIA (Wellcome Trust, United Kingdom); WT080540MA (Wellcome Trust, United Kingdom); 080540 (Wellcome Trust, United Kingdom) | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706268, nn.3140, 10.1038/nn.3140, PMC4050076, EMS58657, 22005678, 16436619, 22706268, 11228837, 17283286, 15501092, 21689594, 19864565, 19805082, 12467598, 21220107, 19904278, 11293691, 19524531, 22006321, 11050020, 16633341, 12814592, 32288199, 21376596, 11488378, 12858179 | Although the ventromedial prefrontal cortex (vmPFC) has long been implicated in reward-guided decision making, its exact role in this process has remained an unresolved issue. Here we show that, in accordance with models of decision making, vmPFC concentrations of GABA and glutamate in human volunteers predict both behavioral performance and the dynamics of a neural value comparison signal. These data provide evidence for a neural competition mechanism in vmPFC that supports value-guided choice. | Adolescent, Adult, Brain Mapping, Choice Behavior, Humans, Inhibition, Psychological, Male, Prefrontal Cortex, Psychomotor Performance, Reward, Young Adult | null |
22,706,269 | 2012-09-04 | 2022-03-09 | 1546-1726 | Nature neuroscience | Segmentation of spatial experience by hippocampal θ sequences. | Gupta Anoopum S, van der Meer Matthijs A A, Touretzky David S, Redish A David | eng | F30 MH091821 (NIMH NIH HHS, United States); R01 MH080318 (NIMH NIH HHS, United States); F30 MH-091821 (NIMH NIH HHS, United States); R01 MH-080318 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22706269, nn.3138, 10.1038/nn.3138, PMC5049882, NIHMS818072, 2592993, 17663452, 9463459, 14736863, 13310704, 16149082, 21270783, 21414906, 22718612, 17532482, 8353611, 15140925, 18716195, 21538659, 19846711, 18448645, 17135403, 19528000, 16161035, 8797016, 4183562, 17229836, 20375279, 16474382, 17700624, 8841826, 11549738, 20130187, 7878473, 10939340, 14687555, 10456097, 19709631, 19615456, 11055443, 19812334, 20223204, 15727540, 17470808, 15078566, 15680687, 17828259, 18425124, 17989284, 16862149, 16600862 | The encoding and storage of experience by the hippocampus is essential for the formation of episodic memories and the transformation of individual experiences into semantic structures such as maps and schemas. The rodent hippocampus compresses ongoing experience into repeating theta sequences, but the factors determining the content of theta sequences are not understood. Here we first show that the spatial paths represented by theta sequences in rats extend farther in front of the rat during acceleration and higher running speeds and begin farther behind the rat during deceleration. Second, the length of the path is directly related to the length of the theta cycle and the number of gamma cycles in it. Finally, theta sequences represent the environment in segments or 'chunks'. These results imply that information encoded in theta sequences is subject to powerful modulation by behavior and task variables. Furthermore, these findings suggest a potential mechanism for the cognitive 'chunking' of experience. | Animals, Hippocampus, Male, Maze Learning, Memory, Episodic, Rats, Rats, Inbred BN, Rats, Inbred F344, Theta Rhythm | null |
22,706,271 | 2012-10-02 | 2021-10-21 | 1735-3408 | Hepatitis monthly | Risk factors of hepatitis B infection: Health policy makers should be aware of their importance in each community. | Miri Seyed Mohammad, Alavian Seyed Moayed | eng | null | Journal Article | null | null | 22706271, PMC3206697, 11872792, 18193122, 21119653, 22087152, 12201870, 19068068, 21196642 | null | null | Hepatitis B infection, Iran, Prevention, Risk factors, Turkey, Vaccination |
22,706,272 | 2012-10-02 | 2022-03-31 | 1735-3408 | Hepatitis monthly | Hepatitis D virus infection in Isfahan, central Iran: Prevalence and risk factors among chronic HBV infection cases. | Ataei Behrooz, Yazdani Mohammad Reza, Kalantari Hamid, Yaran Majid, Nokhodian Zary, Javadi Abbas Ali, Babak Anahita, Adibi Peyman | eng | null | Journal Article | null | null | 22706272, PMC3206699, 8999671, 18629418, 9803612, 3979950, 16174065, 7800870, 3804180, 11003629, 16402678, 16680248, 17098688, 2155876, 8411302, 9580232, 14725415, 1460460, 9195370, 8295493 | Hepatitis D virus (HDV) is dependent on hepatitis B virus (HBV) infection. Acute infection with HDV can occur simultaneously with acute HBV infection or be superimposed onto a chronic HBV infection. | null | Hepatitis B infection, Hepatitis D infection, Iran, Prevalence |
22,706,270 | 2012-09-04 | 2021-10-21 | 1546-1726 | Nature neuroscience | Drosha regulates neurogenesis by controlling neurogenin 2 expression independent of microRNAs. | Knuckles Philip, Vogt Miriam A, Lugert Sebastian, Milo Marta, Chong Mark M W, Hautbergue Guillaume M, Wilson Stuart A, Littman Dan R, Taylor Verdon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Basic Helix-Loop-Helix Transcription Factors, MicroRNAs, Nerve Tissue Proteins, Neurog2 protein, mouse, Drosha protein, mouse, Ribonuclease III | IM | 22706270, nn.3139, 10.1038/nn.3139, 21925378, 19165215, 19089354, 18377642, 20448148, 19703396, 15973356, 16705040, 18690213, 18997113, 22358842, 18725527, 16432209, 15531879, 16314867, 19435891, 17589500, 15046721, 20153966, 17331197, 11239431, 21712401, 16226447, 20816400, 11807030, 20424607, 11896390, 20713509, 18515546, 19783418, 20138502, 16040801, 17679093, 20203209, 20103535, 19026782, 20237275, 19759829, 18400163, 16163386, 17599402, 14703572, 18197166, 15652477, 20620951, 20699384, 18191211, 15574589, 17898002, 21297615, 12175503, 15531877, 19223442, 19135890, 18923076, 21447562 | Temporal regulation of embryonic neurogenesis is controlled by hypostable transcription factors. The mechanism of the process is unclear. Here we show that the RNase III Drosha and DGCR8 (also known as Pasha), key components of the microRNA (miRNA) microprocessor, have important functions in mouse neurogenesis. Loss of microprocessor in forebrain neural progenitors resulted in a loss of stem cell character and precocious differentiation whereas Dicer deficiency did not. Drosha negatively regulated expression of the transcription factors Neurogenin 2 (Ngn2) and NeuroD1 whereas forced Ngn2 expression phenocopied the loss of Drosha. Neurog2 mRNA contains evolutionarily conserved hairpins with similarities to pri-miRNAs, and associates with the microprocessor in neural progenitors. We uncovered a Drosha-dependent destabilization of Neurog2 mRNAs consistent with microprocessor cleavage at hairpins. Our findings implicate direct and miRNA-independent destabilization of proneural mRNAs by the microprocessor, which facilitates neural stem cell (NSC) maintenance by blocking accumulation of differentiation and determination factors. | Animals, Basic Helix-Loop-Helix Transcription Factors, Cells, Cultured, Female, Gene Expression Regulation, Mice, Mice, Inbred C57BL, Mice, Knockout, MicroRNAs, Nerve Tissue Proteins, Neural Stem Cells, Neurogenesis, Pregnancy, Ribonuclease III | null |
22,706,273 | 2012-10-02 | 2021-10-21 | 1735-3408 | Hepatitis monthly | Seroprevalence study of hepatitis A virus in Fars province, southern Iran. | Taghavi Seyed Alireza, Hosseini Asl Mohammad Kazem, Talebzadeh Mozaffar, Eshraghian Ahad | eng | null | Journal Article | null | null | 22706273, PMC3206696, 19842225, 9465507, 9886108, 18290404, 20541138, 18434743, 20606982, 7876643, 15117123, 15635957, 8182265, 12081610, 9620732, 20187662, 11049092 | There are several studies on seroprevalence of hepatitis A virus (HAV) in adults in the Middle East. | null | Epidemiology, Hepatitis A virus, Iran, Seroprevalence |
22,706,274 | 2012-10-02 | 2021-10-21 | 1735-3408 | Hepatitis monthly | New approach to managing occult hepatitis B infection. | Sayan Murat | eng | null | Journal Article | null | null | 22706274, PMC3206695, 16544550, 20382061, 22087120, 22087128 | null | null | Hepatitis, Hepatitis B virus |
22,706,277 | 2013-01-17 | 2013-11-21 | 1460-2083 | Human molecular genetics | Characterization and investigation of zebrafish models of filamin-related myofibrillar myopathy. | Ruparelia Avnika A, Zhao Mo, Currie Peter D, Bryson-Richardson Robert J | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Codon, Nonsense, Filamins, Flncb protein, zebrafish, Microfilament Proteins, Protein Isoforms, Zebrafish Proteins, Myosins | IM | 22706277, dds231, 10.1093/hmg/dds231 | Myofibrillar myopathies are a group of muscle disorders characterized by the disintegration of skeletal muscle fibers and formation of sarcomeric protein aggregates. All the proteins known to be involved in myofibrillar myopathies localize to a region of the sarcomere known as the Z-disk, the site at which defects are first observed. Given the common cellular phenotype observed in this group of disorders, it is thought that there is a common mechanism of pathology. Mutations in filamin C, which has several proposed roles in the development and function of skeletal muscle, can result in filamin-related myofibrillar myopathy. The lack of a suitable animal model system has limited investigation into the mechanism of pathology in this disease and the role of filamin C in muscle development. Here, we characterize stretched out (sot), a zebrafish filamin Cb mutant, together with targeted knockdown of zebrafish filamin Ca, revealing fiber dissolution and formation of protein aggregates strikingly similar to those seen in filamin-related myofibrillar myopathies. Through knockdown of both zebrafish filamin C homologues, we demonstrate that filamin C is not required for fiber specification and that fiber damage is a consequence of muscle activity. The remarkable similarities in the myopathology between our models and filamin-related myofibrillar myopathy makes them suitable for the study of these diseases and provides unique opportunities for the investigation of the function of filamin C in muscle and development of therapies. | Animals, Animals, Genetically Modified, Base Sequence, Body Patterning, Codon, Nonsense, DNA Mutational Analysis, Disease Models, Animal, Embryo, Nonmammalian, Filamins, Gene Expression, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genetic Linkage, Humans, Likelihood Functions, Microfilament Proteins, Muscle Contraction, Muscle Fibers, Slow-Twitch, Muscular Dystrophies, Mutagenesis, Myosins, Phenotype, Phylogeny, Protein Isoforms, Zebrafish, Zebrafish Proteins | null |
22,706,276 | 2013-01-17 | 2022-03-30 | 1460-2083 | Human molecular genetics | Functional analyses of coronary artery disease associated variation on chromosome 9p21 in vascular smooth muscle cells. | Motterle Anna, Pu Xiangyuan, Wood Harriet, Xiao Qingzhong, Gor Shivani, Ng Fu Liang, Chan Kenneth, Cross Frank, Shohreh Beski, Poston Robin N, Tucker Arthur T, Caulfield Mark J, Ye Shu | eng | FS/12/82/29736 (British Heart Foundation, United Kingdom); G9521010 (Medical Research Council, United Kingdom); FS/09/044/28007 (British Heart Foundation, United Kingdom); FS/11/28/28758 (British Heart Foundation, United Kingdom); FS/07/021 (British Heart Foundation, United Kingdom) | Journal Article, Research Support, Non-U.S. Gov't | CDKN2B antisense RNA, human, CDKN2B protein, human, Cyclin-Dependent Kinase Inhibitor p15, Cyclin-Dependent Kinase Inhibitor p16, RNA, Long Noncoding | IM | 22706276, dds224, 10.1093/hmg/dds224, PMC3428153, 19198609, 20056914, 19343170, 17634449, 20395606, 20031606, 18048406, 20502693, 17478679, 20364137, 20173736, 18818403, 20386740, 8181179, 20541999, 18997786, 20670758, 18176561, 21239051, 17478681, 21081134, 21151960, 20729229, 19592466, 18652946, 16239970, 19776743, 7758192, 21804106, 20637465, 19888323, 7648691, 16921403, 18362232, 18032011, 8479518 | Variation on chromosome 9p21 is associated with risk of coronary artery disease (CAD). This genomic region contains the CDKN2A and CDKN2B genes which encode the cell cycle regulators p16(INK4a), p14(ARF) and p15(INK4b) and the ANRIL gene which encodes a non-coding RNA. Vascular smooth muscle cell (VSMC) proliferation plays an important role in the pathogenesis of atherosclerosis which causes CAD. We ascertained whether 9p21 genotype had an influence on CDKN2A/CDKN2B/ANRIL expression levels in VSMCs, VSMC proliferation and VSMC content in atherosclerotic plaques. Immunohistochemical examination showed that VSMCs in atherosclerotic lesions expressed p16(INK4a), p14(ARF) and p15(INK4b). Analyses of primary cultures of VSMCs showed that the 9p21 risk genotype was associated with reduced expression of p16(INK4a), p15(INK4b) and ANRIL (P = 1.2 × 10(-5), 1.4 × 10(-2) and 3.1 × 10(-9)) and with increased VSMC proliferation (P = 1.6 × 10(-2)). Immunohistochemical analyses of atherosclerotic plaques revealed an association of the risk genotype with reduced p15(INK4b) levels in VSMCs (P = 3.7 × 10(-2)) and higher VSMC content (P = 5.6 × 10(-4)) in plaques. The results of this study indicate that the 9p21 variation has an impact on CDKN2A and CDKN2B expression in VSMCs and influences VMSC proliferation, which likely represents an important mechanism for the association between this genetic locus and susceptibility to CAD. | Atherosclerosis, Cell Proliferation, Cells, Cultured, Chromosomes, Human, Pair 9, Coronary Artery Disease, Cyclin-Dependent Kinase Inhibitor p15, Cyclin-Dependent Kinase Inhibitor p16, Gene Expression, Genetic Association Studies, Genotype, Humans, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Plaque, Atherosclerotic, Polymorphism, Single Nucleotide, Primary Cell Culture, RNA, Long Noncoding | null |
22,706,278 | 2013-01-17 | 2023-12-13 | 1460-2083 | Human molecular genetics | Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells. | Lefevre Sophie, Brossas Caroline, Auchère Françoise, Boggetto Nicole, Camadro Jean-Michel, Santos Renata | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Alkylating Agents, Antioxidants, DNA, Fungal, DNA, Mitochondrial, Iron-Binding Proteins, Oxidants, Reactive Oxygen Species, Saccharomyces cerevisiae Proteins, Methyl Methanesulfonate, Hydrogen Peroxide, Endodeoxyribonucleases, Apn1 protein, S cerevisiae, DNA Repair Enzymes, Glutathione | IM | 22706278, dds230, 10.1093/hmg/dds230, PMC3428155, 20649466, 20889968, 20090835, 19563757, 19460301, 20194111, 14697754, 20819074, 9241271, 11238901, 18562474, 19764832, 21511814, 12019217, 18613038, 12483508, 9717241, 10330404, 20156111, 12668611, 6290493, 1693433, 18976116, 12853606, 19158945, 7737504, 17448903, 20373497, 21292986, 8596916, 18184803, 9271239, 20219987, 22155640, 19341380, 21298097, 11328886, 19956589, 10607838, 15661660, 14570713, 21985033, 10207101, 18211957, 15767258, 22352884, 21936771, 19197988, 9180083, 20522547, 21550379, 22287629, 19477695 | Frataxin deficiency results in mitochondrial dysfunction and oxidative stress and it is the cause of the hereditary neurodegenerative disease Friedreich ataxia (FA). Here, we present evidence that one of the pleiotropic effects of oxidative stress in frataxin-deficient yeast cells (Δyfh1 mutant) is damage to nuclear DNA and that repair requires the Apn1 AP-endonuclease of the base excision repair pathway. Major phenotypes of Δyfh1 cells are respiratory deficit, disturbed iron homeostasis and sensitivity to oxidants. These phenotypes are weak or absent under anaerobiosis. We show here that exposure of anaerobically grown Δyfh1 cells to oxygen leads to down-regulation of antioxidant defenses, increase in reactive oxygen species, delay in G1- and S-phases of the cell cycle and damage to mitochondrial and nuclear DNA. Nuclear DNA lesions in Δyfh1 cells are primarily caused by oxidized bases and single-strand breaks that can be detected 15-30 min after oxygen exposition. The Apn1 enzyme is essential for the repair of the DNA lesions in Δyfh1 cells. Compared with Δyfh1, the double Δyfh1Δapn1 mutant shows growth impairment, increased mutagenesis and extreme sensitivity to H(2)O(2). On the contrary, overexpression of the APN1 gene in Δyfh1 cells decreases spontaneous and induced mutagenesis. Our results show that frataxin deficiency in yeast cells leads to increased DNA base oxidation and requirement of Apn1 for repair, suggesting that DNA damage and repair could be important features in FA disease progression. | Alkylating Agents, Anaerobiosis, Antioxidants, Apoptosis, Cell Cycle Checkpoints, DNA Breaks, Double-Stranded, DNA Repair, DNA Repair Enzymes, DNA, Fungal, DNA, Mitochondrial, Endodeoxyribonucleases, Friedreich Ataxia, Gene Expression, Gene Expression Regulation, Fungal, Glutathione, Humans, Hydrogen Peroxide, Iron-Binding Proteins, Methyl Methanesulfonate, Microbial Viability, Mutagenesis, Oxidants, Oxidation-Reduction, Oxidative Stress, Reactive Oxygen Species, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Frataxin | null |
22,706,279 | 2013-07-08 | 2016-11-25 | 1535-7228 | The American journal of psychiatry | Genetic variation in KCNH2 associated with expression in the brain of a unique hERG isoform modulates treatment response in patients with schizophrenia. | Apud José A, Zhang Fengyu, Decot Heather, Bigos Kristin L, Weinberger Daniel R | eng | null | Journal Article | Antipsychotic Agents, ERG1 Potassium Channel, Ether-A-Go-Go Potassium Channels, KCNH2 protein, human, Protein Isoforms | IM | 22706279, 1182558, 10.1176/appi.ajp.2012.11081214 | Antidopaminergic drugs bind to hERG1 potassium channels encoded by the gene KCNH2, which accounts for the side effect of QT interval prolongation. KCNH2 has also been associated with schizophrenia risk, and risk alleles predict increased expression of a brain-selective isoform, KCNH2 3.1, that has unique physiological properties. The authors assessed whether genetic variation associated with KCNH2 3.1 expression influences the therapeutic effects of antipsychotic drugs. | Adult, Alleles, Antipsychotic Agents, Clinical Trials as Topic, ERG1 Potassium Channel, Ether-A-Go-Go Potassium Channels, Female, Genetic Variation, Genotype, Humans, Linkage Disequilibrium, Male, Protein Isoforms, Schizophrenia | null |
22,706,280 | 2013-05-23 | 2012-12-03 | 1638-6183 | Biochimie | Bacterial cell wall macroamphiphiles: pathogen-/microbe-associated molecular patterns detected by mammalian innate immune system. | Ray Aurélie, Cot Marlène, Puzo Germain, Gilleron Martine, Nigou Jérôme | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Lipopolysaccharides, TLR2 protein, human, TLR4 protein, human, Teichoic Acids, Toll-Like Receptor 2, Toll-Like Receptor 4, lipoteichoic acid | IM | 22706280, S0300-9084(12)00241-6, 10.1016/j.biochi.2012.06.007 | Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell wall macroamphiphiles, namely Gram-negative bacteria lipopolysaccharide (LPS), Gram-positive bacteria lipoteichoic acid (LTA), lipoproteins and mycobacterial lipoglycans, are important molecules for the physiology of bacteria and evidently meet PAMP/MAMP criteria. They are well suited to innate immune recognition and constitute non-self signatures detected by the innate immune system to signal the presence of an infective agent. They are notably recognized via their lipid anchor by Toll-like receptors (TLRs) 4 or 2. Here, we review our current knowledge of the molecular bases of macroamphiphile recognition by TLRs, with a special emphasis on mycobacterial lipoglycan detection by TLR2. | Bacteria, Cell Wall, Host-Parasite Interactions, Humans, Immunity, Innate, Lipopolysaccharides, Teichoic Acids, Toll-Like Receptor 2, Toll-Like Receptor 4 | null |
22,706,281 | 2013-03-05 | 2020-05-01 | 1638-6183 | Biochimie | Cilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2. | Munisso Maria Chiara, Kang Jeong-Hun, Tsurufuji Makoto, Yamaoka Tetsuji | eng | null | Journal Article | BMP2 protein, human, Bone Morphogenetic Protein 2, Core Binding Factor Alpha 1 Subunit, Cyclohexanecarboxylic Acids, Isoquinolines, Nitriles, Phosphodiesterase 4 Inhibitors, Smad Proteins, Sp7 protein, rat, Sulfonamides, Transcription Factors, Osteocalcin, Cilomilast, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Alkaline Phosphatase, Cyclic Nucleotide Phosphodiesterases, Type 4, N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide | IM | 22706281, S0300-9084(12)00234-9, 10.1016/j.biochi.2012.05.031 | A rapid and efficient method to stimulate bone regeneration would be useful in orthopaedic stem cell therapies. Rolipram is an inhibitor of phosphodiesterase 4 (PDE4), which mediates cyclic adenosine monophosphate (cAMP) degradation. Systemic injection of rolipram enhances osteogenesis induced by bone morphogenetic protein 2 (BMP-2) in mice. However, there is little data on the precise mechanism, by which the PDE4 inhibitor regulates osteoblast gene expression. In this study, we investigated the combined ability of BMP-2 and cilomilast, a second-generation PDE4 inhibitor, to enhance the osteoblastic differentiation of mesenchymal stem cells (MSCs). The alkaline phosphatase (ALP) activity of MSCs treated with PDE4 inhibitor (cilomilast or rolipram), BMP-2, and/or H89 was compared with the ALP activity of MSCs differentiated only by osteogenic medium (OM). Moreover, expression of Runx2, osterix, and osteocalcin was quantified using real-time polymerase chain reaction (RT-PCR). It was found that cilomilast enhances the osteoblastic differentiation of MSCs equally well as rolipram in primary cultured MSCs. Moreover, according to the H89 inhibition experiments, Smad pathway was found to be an important signal transduction pathway in mediating the osteogenic effect of BMP-2, and this effect is intensified by an increase in cAMP levels induced by PDE4 inhibitor. | Alkaline Phosphatase, Animals, Bone Morphogenetic Protein 2, Calcification, Physiologic, Cell Differentiation, Core Binding Factor Alpha 1 Subunit, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Cyclic Nucleotide Phosphodiesterases, Type 4, Cyclohexanecarboxylic Acids, Drug Interactions, Gene Expression Regulation, Humans, Isoquinolines, Male, Mesenchymal Stem Cells, Nitriles, Osteoblasts, Osteocalcin, Osteogenesis, Phosphodiesterase 4 Inhibitors, Rats, Rats, Wistar, Signal Transduction, Smad Proteins, Sulfonamides, Transcription Factors | null |
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