PMID
int64 275k
40M
| DateCompleted
stringdate 1965-11-14 00:00:00
2025-02-28 00:00:00
⌀ | DateRevised
stringdate 2000-12-18 00:00:00
2025-02-28 00:00:00
| ISSN
stringlengths 9
9
⌀ | JournalTitle
stringlengths 2
239
| ArticleTitle
stringlengths 1
1.99k
⌀ | Authors
stringlengths 3
61.6k
⌀ | Language
stringclasses 47
values | Grants
stringlengths 11
10.6k
⌀ | PublicationTypes
stringlengths 4
259
| Chemicals
stringlengths 3
1.33k
⌀ | CitationSubset
stringclasses 2
values | ArticleIds
stringlengths 6
44.3k
| Abstract
stringlengths 1
16.7k
⌀ | MeshTerms
stringlengths 3
1.18k
⌀ | Keywords
stringlengths 1
17.2k
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22,706,066 | 2012-12-11 | 2021-10-21 | 1098-5336 | Applied and environmental microbiology | Emergent macrophytes act selectively on ammonia-oxidizing bacteria and archaea. | Trias Rosalia, Ruiz-Rueda Olaya, García-Lledó Arantzazu, Vilar-Sanz Ariadna, López-Flores Rocío, Quintana Xavier D, Hallin Sara, Bañeras Lluís | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Ammonia | IM | 22706066, AEM.00919-12, 10.1128/AEM.00919-12, PMC3416627, 17953555, 22158986, 20421470, 19049502, 20889787, 21239545, 21196023, 18325029, 19794413, 19304820, 21525411, 11125085, 16186488, 21083579, 16915287, 16309395, 21364937, 17686020, 19453522, 20370827, 21039647, 18344332, 11055931, 22081571, 16024557, 18336563, 20389005, 20097811 | Ammonia-oxidizing bacteria (AOB) and archaea (AOA) were quantified in the sediments and roots of dominant macrophytes in eight neutral to alkaline coastal wetlands. The AOA dominated in most samples, but the bacterial-to-archaeal amoA gene ratios increased with increasing ammonium levels and pH in the sediments. For all plant species, the ratios increased on the root surface relative to the adjacent bulk sediment. This suggests that root surfaces in these environments provide conditions favoring enrichment of AOB. | Ammonia, Archaea, Bacteria, Biodiversity, Hydrogen-Ion Concentration, Metagenome, Oxidation-Reduction, Plant Roots, Wetlands | null |
22,706,067 | 2012-12-11 | 2021-10-21 | 1098-5336 | Applied and environmental microbiology | Identification and biochemical evidence of a medium-chain-length polyhydroxyalkanoate depolymerase in the Bdellovibrio bacteriovorus predatory hydrolytic arsenal. | Martínez Virginia, de la Peña Fernando, García-Hidalgo Javier, de la Mata Isabel, García José Luis, Prieto María Auxiliadora | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Enzyme Inhibitors, Polyhydroxyalkanoates, Polysorbates, Recombinant Proteins, Phenylmethylsulfonyl Fluoride, Carboxylic Ester Hydrolases, poly(3-hydroxyalkanoic acid) depolymerase, Dithiothreitol | IM | 22706067, AEM.01099-12, 10.1128/AEM.01099-12, PMC3416617, 17170116, 21751398, 10924740, 20400568, 8096622, 21069589, 19575566, 12855176, 12618469, 12831901, 21326335, 21811488, 10940016, 8476295, 11457823, 14068454, 7961472, 5432063, 19296857, 22225632, 15489436, 10066830, 21274528, 8910058, 6950388, 3110136, 942051, 17483792, 10943401, 12213937, 12534460, 12558595, 18836011, 21845385, 16413191, 14752164, 10416666, 15986831, 16374769, 11114905, 19267463, 12954080, 20393709, 8837471, 10517528, 10493927, 11501670, 9889311, 15263901, 3041370, 17546510, 19788655 | The obligate predator Bdellovibrio bacteriovorus HD100 shows a large set of proteases and other hydrolases as part of its hydrolytic arsenal needed for its predatory life cycle. We present genetic and biochemical evidence that open reading frame (ORF) Bd3709 of B. bacteriovorus HD100 encodes a novel medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase (PhaZ(Bd)). The primary structure of PhaZ(Bd) suggests that this enzyme belongs to the α/β-hydrolase fold family and has a typical serine hydrolase catalytic triad (serine-histidine-aspartic acid) in agreement with other PHA depolymerases and lipases. PhaZ(Bd) has been extracellularly produced using different hypersecretor Tol-pal mutants of Escherichia coli and Pseudomonas putida as recombinant hosts. The recombinant PhaZ(Bd) has been characterized, and its biochemical properties have been compared to those of other PHA depolymerases. The enzyme behaves as a serine hydrolase that is inhibited by phenylmethylsulfonyl fluoride. It is also affected by the reducing agent dithiothreitol and nonionic detergents like Tween 80. PhaZ(Bd) is an endoexohydrolase that cleaves both large and small PHA molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades mcl-PHA and is inactive toward short-chain-length polyhydroxyalkanoates (scl-PHA) like polyhydroxybutyrate (PHB). These studies shed light on the potentiality of these predators as sources of new biocatalysts, such as an mcl-PHA depolymerase, for the production of enantiopure hydroxyalkanoic acids and oligomers as building blocks for the synthesis of biobased polymers. | Amino Acid Sequence, Bdellovibrio, Carboxylic Ester Hydrolases, Dithiothreitol, Enzyme Inhibitors, Escherichia coli, Hydrolysis, Open Reading Frames, Phenylmethylsulfonyl Fluoride, Polyhydroxyalkanoates, Polysorbates, Pseudomonas putida, Recombinant Proteins, Sequence Homology, Amino Acid, Substrate Specificity | null |
22,706,069 | 2012-12-18 | 2022-03-11 | 1473-6586 | Current opinion in urology | Novel methods for renal tissue ablation. | Olweny Ephrem O, Cadeddu Jeffrey A | eng | null | Journal Article, Review | null | IM | 22706069, 10.1097/MOU.0b013e328355ecf5 | To provide an overview of the current research on renal tissue ablation, highlighting novel ablation techniques and technologies. | Ablation Techniques, Catheter Ablation, Cryosurgery, Electrochemotherapy, High-Intensity Focused Ultrasound Ablation, Humans, Kidney Diseases, Kidney Neoplasms, Treatment Outcome | null |
22,706,070 | 2012-12-18 | 2016-11-25 | 1473-6586 | Current opinion in urology | Risk prediction in the management of small renal masses. | Bagrodia Aditya, Darwish Oussama M, Rapoport Yury, Margulis Vitaly | eng | null | Journal Article, Review | null | IM | 22706070, 10.1097/MOU.0b013e328355ede9 | Small renal masses (SRMs) are frequently encountered due to the ubiquitous use of abdominal cross-sectional imaging. Enhanced risk prediction in the management of SRMs would allow for a more informed decision of which, if any, patients would benefit from the available intervention modalities. | Biopsy, Disease Management, Humans, Kidney, Kidney Diseases, Kidney Neoplasms, Nephrectomy, Prognosis, Radiography, Risk Factors | null |
22,706,071 | 2012-10-25 | 2019-12-10 | 1533-4058 | Applied immunohistochemistry & molecular morphology : AIMM | Immunohistochemistry cocktails are here to stay: Center of Medicare and Medicaid Services should revise its new reimbursement policy. | Yaziji Hadi, Eisen Richard, Wick Mark, Swanson Paul, Badve Sunil, Cartun Richard, Haas Thomas, Marolt Monna, Hicks David, Martin Alvin, Barry Todd, Alsabeh Randa, Taylor James, Fulton Regan, Goldsmith Jeffrey, Shen Steven, Taylor Clive | eng | null | Editorial | Antigens, CD, Antigens, Neoplasm | IM | 22706071, 10.1097/PAI.0b013e31825ed8a5, 00129039-201207000-00001 | null | Antigens, CD, Antigens, Neoplasm, Centers for Medicare and Medicaid Services, U.S., Cost-Benefit Analysis, Diagnostic Errors, Humans, Immunohistochemistry, Medicaid, Medicare, Neoplasms, Reimbursement Mechanisms, United States | null |
22,706,073 | 2012-10-30 | 2012-07-20 | 1872-9142 | Molecular immunology | CɛmX peptide-carrying HBcAg virus-like particles induced antibodies that down-regulate mIgE-B lymphocytes. | Lin Chien-Jen, Chen Nien-Yi, Chen Jiun-Bo, Lu Chien-Sheng, Hung Alfur Fu-Hsin, Shiung Yu-Yu, Wu Pheidias C, Pan Rong-Long, Chang Tse Wen | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Hepatitis B Core Antigens, Peptides, Receptors, Antigen, B-Cell, Vaccines, Virus-Like Particle, Immunoglobulin E | IM | 22706073, S0161-5890(12)00316-1, 10.1016/j.molimm.2012.05.015 | Type-I hypersensitivity reactions play a critical role in the pathogenesis of various allergic diseases. The successful development of the anti-IgE antibody, omalizumab, has validated IgE as an effective therapeutic target for the treatment of various IgE-mediated allergic diseases. Two research groups have reported that mAbs specific for certain parts of CɛmX, a domain of 52 aa residues in human membrane-bound IgE (mIgE), can cause the lysis of mIgE-B cells by apoptosis and antibody-dependent cellular cytotoxicity (ADCC). Herein, we explore virus-like particles formed by hepatitis B virus core antigen (HBcAg) that harbors the entire CɛmX peptide or its fragments as immunogens for inducing anti-CɛmX antibodies. The results showed that mice immunized subcutaneously with these immunogens produced antibodies that bind to recombinant CɛmX-containing human IgE.Fc in ELISA and Western blot analyses, and to genetically engineered human mIgE-expressing Ramos B cell line in fluorescence flow cytometric assays. The IgG antibodies purified from the sera of immunized mice were able to cause the apoptosis of mIgE-expressing Ramos cells through a BCR-dependent caspase pathway. Furthermore, the IgG could mediate ADCC in human mIgE-expressing A20 murine B-cell lymphoma. These studies suggest that HBcAg-CɛmX peptide immunogens warrant further investigation as a therapeutic modality for modulating IgE in patients with IgE-mediated allergic diseases. | Animals, Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Antibody-Dependent Cell Cytotoxicity, Apoptosis, B-Lymphocytes, Cell Line, Tumor, Down-Regulation, Female, Hepatitis B Core Antigens, Hepatitis B virus, Humans, Immunoglobulin E, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Peptides, Receptors, Antigen, B-Cell, Vaccines, Virus-Like Particle | null |
22,706,074 | 2012-10-16 | 2013-11-21 | 1464-0333 | Journal of environmental monitoring : JEM | Preparation and measurement methods for studying nanoparticle aggregate surface chemistry. | Szakal Christopher, McCarthy James A, Ugelow Melissa S, Konicek Andrew R, Louis Kacie, Yezer Benjamin, Herzing Andrew A, Hamers Robert J, Holbrook R David | eng | null | Journal Article | titanium dioxide, Titanium | IM | 22706074, 10.1039/c2em30048f | Despite best efforts at controlling nanoparticle (NP) surface chemistries, the environment surrounding nanomaterials is always changing and can impart a permanent chemical memory. We present a set of preparation and measurement methods to be used as the foundation for studying the surface chemical memory of engineered NP aggregates. We attempt to bridge the gap between controlled lab studies and real-world NP samples, specifically TiO(2), by using well-characterized and consistently synthesized NPs, controllably producing NP aggregates with precision drop-on-demand inkjet printing for subsequent chemical measurements, monitoring the physical morphology of the NP aggregate depositions with scanning electron microscopy (SEM), acquiring "surface-to-bulk" mass spectra of the NP aggregate surfaces with time-of-flight secondary ion mass spectrometry (ToF-SIMS), and developing a data analysis scheme to interpret chemical signatures more accurately from thousands of data files. We present differences in mass spectral peak ratios for bare TiO(2) NPs compared to NPs mixed separately with natural organic matter (NOM) or pond water. The results suggest that subtle changes in the local environment can alter the surface chemistry of TiO(2) NPs, as monitored by Ti(+)/TiO(+) and Ti(+)/C(3)H(5)(+) peak ratios. The subtle changes in the absolute surface chemistry of NP aggregates vs. that of the subsurface are explored. It is envisioned that the methods developed herein can be adapted for monitoring the surface chemistries of a variety of engineered NPs obtained from diverse natural environments. | Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Models, Molecular, Nanoparticles, Particle Size, Surface Properties, Titanium | null |
22,706,076 | 2012-09-20 | 2012-07-09 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Structural characterization and inhibitory profile of formyl peptide receptor 2 selective peptides descending from a PIP2-binding domain of gelsolin. | Forsman Huamei, Andréasson Emil, Karlsson Jennie, Boulay Francois, Rabiet Marie-Josèphe, Dahlgren Claes | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Adaptor Proteins, Signal Transducing, Cytoskeletal Proteins, FPR2 protein, human, Gelsolin, PSTPIP2 protein, human, Peptides, Receptors, Formyl Peptide, Receptors, Lipoxin | IM | 22706076, jimmunol.1101616, 10.4049/jimmunol.1101616 | The neutrophil formyl peptide receptors, FPR1 and FPR2, play critical roles for inflammatory reactions, and receptor-specific antagonists/inhibitors can possibly be used to facilitate the resolution of pathological inflammatory reactions. A 10-aa-long rhodamine-linked and membrane-permeable peptide inhibitor (PBP10) has such a potential. This FPR2 selective inhibitor adopts a phosphatidylinositol 4,5-bisphosphate-binding sequence in the cytoskeletal protein gelsolin. A core peptide, RhB-QRLFQV, is identified that displays inhibitory effects as potent as the full-length molecule. The phosphatidylinositol 4,5-bisphosphate-binding capacity of PBP10 was not in its own sufficient for inhibition. A receptor in which the presumed cytoplasmic signaling C-terminal tail of FPR2 was replaced with that of FPR1 retained the PBP10 sensitivity, suggesting that the tail of FPR2 was not on its own critical for inhibition. This gains support from the fact that the effect of cell-penetrating lipopeptide (a pepducin), suggested to act primarily through the third intracellular loop of FPR2, was significantly inhibited by PBP10. The third intracellular loops of FPR1 and FPR2 differ in only two amino acids, but an FPR2 mutant in which these two amino acids were replaced by those present in FPR1 retained the PBP10 sensitivity. In summary, we conclude that the inhibitory activity on neutrophil function of PBP10 is preserved in the core sequence RhB-QRLFQV and that neither the third intracellular loop of FPR2 nor the cytoplasmic tail of the receptor alone is responsible for the specific inhibition. | Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Cell Membrane Permeability, Cytoskeletal Proteins, Dose-Response Relationship, Immunologic, Gelsolin, HL-60 Cells, Humans, Molecular Sequence Data, Neutrophil Activation, Peptides, Protein Binding, Protein Structure, Tertiary, Receptors, Formyl Peptide, Receptors, Lipoxin | null |
22,706,075 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Changes in functional but not structural avidity during differentiation of CD8+ effector cells in vivo after virus infection. | Amoah Samuel, Yammani Rama D, Grayson Jason M, Alexander-Miller Martha A | eng | R01 AI043591 (NIAID NIH HHS, United States); R01 HL071985 (NHLBI NIH HHS, United States) | Comparative Study, Journal Article, Research Support, N.I.H., Extramural | CD8 Antigens, Receptors, Antigen, T-Cell | IM | 22706075, jimmunol.1102579, 10.4049/jimmunol.1102579, PMC3397685, NIHMS378644, 20053740, 8633023, 1506684, 12244138, 12496398, 20636815, 19539966, 20096607, 9916724, 11786907, 20096608, 11698456, 11390443, 20504887, 21264852, 20103768, 17892848, 17114419, 11509598, 17723218, 12368035, 17617563, 16273099, 18524828, 18323851, 8962124, 12614358, 16163673, 15731228, 12938231, 14625547, 19966302, 18316415, 9655486, 15135297, 19664942, 9973498, 17893201, 19380819, 18082432, 17484768, 1634779, 18712791, 19389882, 9584143, 10846055, 11477407, 20696858, 7677954 | By the peak of the CD8(+) T cell response, the effector cell pool consists of a heterogeneous population of cells that includes both those with an increased propensity to become long-lived memory cells (memory precursor effector cells; MPEC) and those that are terminally differentiated cells (short-lived effector cells; SLEC). Numerous studies have established the critical role that functional avidity plays in determining the in vivo efficacy of CD8(+) effector cells. Currently, how functional avidity differs in MPEC versus SLEC and the evolution of this property within these two populations during the expansion and contraction of the response are unknown. The data presented in this study show that at the peak of the effector response generated after poxvirus infection, SLEC were of higher functional avidity than their MPEC counterpart. Over time, however, SLEC exhibited a decrease in peptide sensitivity. This is in contrast to MPEC, which showed a modest increase in peptide sensitivity as the response reached equilibrium. The decrease in functional avidity in SLEC was independent of CD8 modulation or the amount of Ag receptor expressed by the T cell. Instead, the loss in sensitivity was correlated with decreased expression and activation of ZAP70 and Lck, critical components of TCR membrane proximal signaling. These results highlight the potential contribution of avidity in the differentiation and evolution of the T cell effector response after viral infection. | Animals, Antigen-Presenting Cells, CD8 Antigens, CD8-Positive T-Lymphocytes, Cell Adhesion, Cell Differentiation, Cellular Senescence, Immunologic Memory, Lymphocytic choriomeningitis virus, Mice, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, Signal Transduction, Time Factors, Vaccinia virus | null |
22,706,072 | 2013-01-16 | 2012-08-27 | 1879-3649 | Vascular pharmacology | Selective glycolysis blockade in guinea pig pulmonary artery and aorta reverses contractile and electrical responses to acute hypoxia. | Soloviev Anatoly I, Bondarenko Alexander I, Kizub Igor V | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706072, S1537-1891(12)00122-X, 10.1016/j.vph.2012.06.001 | The goal of this study was to clarify the mechanisms of hypoxic pulmonary vasoconstriction (HPV) reversal following selective glycolysis blockade and to assess possible contribution of endothelial electrogenesis to this phenomenon as a trigger mechanism. We compared smooth muscle (SM) contractility and endothelial cell (EC) membrane potential (MP) during acute hypoxia before and after glycolysis blockade. MPs were recorded from the endothelium of guinea pig pulmonary artery (GPPA) and thoracic aorta (GPTA) using the patch-clamp technique. Acute hypoxia caused hyperpolarization in GPTA EC, while EC from GPPA were depolarized. Also, acute hypoxia elicited constriction in isolated GPPA and dilatation in GPTA. Selective glycolysis inhibition always reversed both electrical and contractile responses in GPPA to hypoxia, but in GPTA this only occurred in 30% of experiments. It is likely that an unknown glycolysis-driven mechanism in EC mediates vascular tone regulation under hypoxia and underlies the paradoxical difference in the response of pulmonary and systemic arterial SM to hypoxia. Our data suggest that HPV development in GPPA might, at least partially, be driven by EC depolarization spreading to the underlying SM cells. | Animals, Aorta, Thoracic, Cell Hypoxia, Endothelial Cells, Glycolysis, Guinea Pigs, Membrane Potentials, Muscle Contraction, Muscle, Smooth, Vascular, Patch-Clamp Techniques, Pulmonary Artery, Vasoconstriction | null |
22,706,077 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Gemfibrozil, a lipid-lowering drug, upregulates IL-1 receptor antagonist in mouse cortical neurons: implications for neuronal self-defense. | Corbett Grant T, Roy Avik, Pahan Kalipada | eng | R21 NS071479 (NINDS NIH HHS, United States); T32 AG000269 (NIA NIH HHS, United States); T32AG00269 (NIA NIH HHS, United States); NS71479 (NINDS NIH HHS, United States); NS64564 (NINDS NIH HHS, United States); R01 AT006681 (NCCIH NIH HHS, United States); R21 NS064564 (NINDS NIH HHS, United States); AT6681 (NCCIH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Apoptosis Regulatory Proteins, Hypolipidemic Agents, Inflammation Mediators, Interleukin 1 Receptor Antagonist Protein, Phosphatidylinositol 3-Kinase, Proto-Oncogene Proteins c-akt, Gemfibrozil | IM | 22706077, jimmunol.1102624, 10.4049/jimmunol.1102624, PMC3523125, NIHMS423450, 7876895, 9829964, 18040843, 10202538, 21414557, 10438259, 20032313, 11279236, 17785853, 11584311, 8001745, 20861373, 16859761, 15489356, 15630430, 10652539, 10858586, 17549256, 15201366, 2166677, 11701324, 19812204, 12220547, 15197750, 15240729, 9674758, 11268266, 12660731, 19864567, 15378607, 11754997, 17625103, 16109311, 10379022, 10753867, 9005852, 10381209, 16542445, 12431424, 8423067, 14507968, 8313151, 18302763, 19822205, 9597123, 17428507, 17638387, 19596989, 15509740, 10364511, 16929354, 11455132, 12244038, 19694602, 11596125, 15452132, 10600750 | Chronic inflammation is becoming a hallmark of several neurodegenerative disorders and accordingly, IL-1β, a proinflammatory cytokine, is implicated in the pathogenesis of neurodegenerative diseases. Although IL-1β binds to its high-affinity receptor, IL-1R, and upregulates proinflammatory signaling pathways, IL-1R antagonist (IL-1Ra) adheres to the same receptor and inhibits proinflammatory cell signaling. Therefore, upregulation of IL-1Ra is considered important in attenuating inflammation. The present study underlines a novel application of gemfibrozil (gem), a Food and Drug Administration-approved lipid-lowering drug, in increasing the expression of IL-1Ra in primary mouse and human neurons. Gem alone induced an early and pronounced increase in the expression of IL-1Ra in primary mouse cortical neurons. Activation of type IA p110α PI3K and Akt by gem and abrogation of gem-induced upregulation of IL-1Ra by inhibitors of PI3K and Akt indicate a role of the PI3K-Akt pathway in the upregulation of IL-1Ra. Gem also induced the activation of CREB via the PI3K-Akt pathway, and small interfering RNA attenuation of CREB abolished the gem-mediated increase in IL-1Ra. Furthermore, gem was able to protect neurons from IL-1β insult. However, small interfering RNA knockdown of neuronal IL-1Ra abrogated the protective effect of gem against IL-1β, suggesting that this drug increases the defense mechanism of cortical neurons via upregulation of IL-1Ra. Taken together, these results highlight the importance of the PI3K-Akt-CREB pathway in mediating gem-induced upregulation of IL-1Ra in neurons and suggest gem as a possible therapeutic treatment for propagating neuronal self-defense in neuroinflammatory and neurodegenerative disorders. | Animals, Apoptosis Regulatory Proteins, Cells, Cultured, Cerebral Cortex, Gemfibrozil, Humans, Hypolipidemic Agents, Inflammation Mediators, Interleukin 1 Receptor Antagonist Protein, Mice, Mice, Inbred C57BL, Neurodegenerative Diseases, Neurons, Phosphatidylinositol 3-Kinase, Proto-Oncogene Proteins c-akt, Signal Transduction, Up-Regulation | null |
22,706,078 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Platelets present antigen in the context of MHC class I. | Chapman Lesley M, Aggrey Angela A, Field David J, Srivastava Kalyan, Ture Sara, Yui Katsuyuki, Topham David J, Baldwin William M, Morrell Craig N | eng | R01HL093179 (NHLBI NIH HHS, United States); P01 AI087586 (NIAID NIH HHS, United States); R01 HL093179 (NHLBI NIH HHS, United States); R01HL094547 (NHLBI NIH HHS, United States); P01AI087586 (NIAID NIH HHS, United States); R01 HL094547 (NHLBI NIH HHS, United States); R01HL093179-02S109 (NHLBI NIH HHS, United States); TL1 RR024135 (NCRR NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Histocompatibility Antigens Class I | IM | 22706078, jimmunol.1200580, 10.4049/jimmunol.1200580, PMC3392496, NIHMS378653, 15968380, 20003396, 18283118, 12574396, 17519413, 16438672, 16322783, 8025267, 9591709, 9175783, 15673159, 16373597, 16442516, 16369021, 14173797, 12850839, 12010803, 19067663, 16678181, 12676820, 6485052, 16096058, 8248811, 12552430, 8662511, 18665903, 15494426, 1390243, 20962257, 15678283, 1409624, 14567912, 16498500, 9358766, 16440342, 21436837, 21494565, 8977634, 16600241, 989635, 21596849, 16940507, 8639783, 20107233, 16304048, 18692777, 15738422, 56961, 12362238, 14962480, 20454664, 20852838, 16618794, 9350300, 18606696, 16258538, 16979941, 4582662, 18799734 | Platelets are most recognized for their vital role as the cellular mediator of thrombosis, but platelets also have important immune functions. Platelets initiate and sustain vascular inflammation in many disease conditions, including arthritis, atherosclerosis, transplant rejection, and severe malaria. We now demonstrate that platelets express T cell costimulatory molecules, process and present Ag in MHC class I, and directly activate naive T cells in a platelet MHC class I-dependent manner. Using an experimental cerebral malaria mouse model, we also demonstrate that platelets present pathogen-derived Ag to promote T cell responses in vivo, and that platelets can be used in a cell-based vaccine model to induce protective immune responses. Our study demonstrates a novel Ag presentation role for platelets. | Animals, Antigen Presentation, Blood Platelets, CD8-Positive T-Lymphocytes, Cell Movement, Histocompatibility Antigens Class I, Humans, Lymphocyte Activation, Malaria, Mice, Mice, Inbred C57BL, Plasmodium berghei | null |
22,706,080 | 2012-09-20 | 2022-03-17 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | TGF-β mediates proinflammatory seminal fluid signaling in human cervical epithelial cells. | Sharkey David J, Macpherson Anne M, Tremellen Kelton P, Mottershead David G, Gilchrist Robert B, Robertson Sarah A | eng | null | Journal Article | Cytokines, Inflammation Mediators, Protein Isoforms, Transforming Growth Factor beta, Transforming Growth Factor beta1, Transforming Growth Factor beta2, Transforming Growth Factor beta3 | IM | 22706080, jimmunol.1200005, 10.4049/jimmunol.1200005 | The cervix is central to the female genital tract immune response to pathogens and foreign male Ags introduced at coitus. Seminal fluid profoundly influences cervical immune function, inducing proinflammatory cytokine synthesis and leukocyte recruitment. In this study, human Ect1 cervical epithelial cells and primary cervical cells were used to investigate agents in human seminal plasma that induce a proinflammatory response. TGF-β1, TGF-β2, and TGF-β3 are abundant in seminal plasma, and Affymetrix microarray revealed that TGF-β3 elicits changes in Ect1 cell expression of several proinflammatory cytokine and chemokine genes, replicating principal aspects of the Ect1 response to seminal plasma. The differentially expressed genes included several induced in the physiological response of the cervix to seminal fluid in vivo. Notably, all three TGF-β isoforms showed comparable ability to induce Ect1 cell expression of mRNA and protein for GM-CSF and IL-6, and TGF-β induced a similar IL-6 and GM-CSF response in primary cervical epithelial cells. TGF-β neutralizing Abs, receptor antagonists, and signaling inhibitors ablated seminal plasma induction of GM-CSF and IL-6, but did not alter IL-8, CCL2 (MCP-1), CCL20 (MIP-3α), or IL-1α production. Several other cytokines present in seminal plasma did not elicit Ect1 cell responses. These data identify all three TGF-β isoforms as key agents in seminal plasma that signal induction of proinflammatory cytokine synthesis in cervical cells. Our findings suggest that TGF-β in the male partner's seminal fluid may influence cervical immune function after coitus in women, and potentially be a determinant of fertility, as well as defense from infection. | 3T3 Cells, Animals, Cells, Cultured, Cervix Uteri, Cytokines, Epithelial Cells, Female, Humans, Inflammation Mediators, Male, Mice, Protein Isoforms, Semen, Signal Transduction, Transforming Growth Factor beta, Transforming Growth Factor beta1, Transforming Growth Factor beta2, Transforming Growth Factor beta3 | null |
22,706,079 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Differential idiotype utilization for the in vivo type 14 capsular polysaccharide-specific Ig responses to intact Streptococcus pneumoniae versus a pneumococcal conjugate vaccine. | Colino Jesus, Duke Leah, Arjunaraja Swadhinya, Chen Quanyi, Liu Leyu, Lucas Alexander H, Snapper Clifford M | eng | R01 AI025008 (NIAID NIH HHS, United States); R37 AI025008 (NIAID NIH HHS, United States); AI25008 (NIAID NIH HHS, United States); R01 AI049192 (NIAID NIH HHS, United States); AI49192 (NIAID NIH HHS, United States) | Comparative Study, Journal Article, Research Support, N.I.H., Extramural | Antibodies, Monoclonal, Antigens, Bacterial, Immunoglobulin Idiotypes, Pneumococcal Vaccines, Vaccines, Conjugate, pneumococcal polysaccharide, type 14 | IM | 22706079, jimmunol.1200599, 10.4049/jimmunol.1200599, PMC3392520, NIHMS377166, 12242446, 3944471, 6662190, 8609400, 17043104, 22523389, 18025197, 12033738, 3081651, 2704374, 22120117, 11781360, 1848692, 3115792, 16039575, 17548615, 2471648, 7706754, 18691914, 1707658, 6201106, 2957437, 8805617, 12846803, 19502021, 10760811, 19005020, 3912370, 22288494, 16116197, 8450060, 12117915, 2215183, 15771569, 15003659, 8843627, 21030, 10569753, 1309720, 16861066, 11895934, 18710933, 19570830, 1752943, 7612238, 2471773, 5804533, 1760840, 18678667, 15155658, 6809623, 15824068, 14766417, 9393802, 9573085, 19855403, 8047849, 1956400, 20018621, 1560200, 15618166, 17345580, 19201854, 9341782, 11381099, 11159978, 16918444, 10837415, 2011583, 8506338, 16860443, 22101769, 7681079, 21522065, 15357949, 3298228, 4120094, 16169502, 6967514 | Murine IgG responses specific for the capsular polysaccharide (pneumococcal capsular polysaccharide serotype 14; PPS14) of Streptococcus pneumoniae type 14 (Pn14), induced in response to intact Pn14 or a PPS14-protein conjugate, are both dependent on CD4(+) T cell help but appear to use marginal zone versus follicular B cells, respectively. In this study, we identify an idiotype (44.1-Id) that dominates the PPS14-specific IgG, but not IgM, responses to intact Pn14, isolated PPS14, and Group B Streptococcus (strain COH1-11) expressing capsular polysaccharide structurally identical to PPS14. The 44.1-Id, however, is not expressed in the repertoire of natural PPS14-specific Abs. In distinct contrast, PPS14-specific IgG responses to a soluble PPS14-protein conjugate exhibit minimal usage of the 44.1-Id, although significant 44.1-Id expression is elicited in response to conjugate attached to particles. The 44.1-Id elicited in response to intact Pn14 was expressed in similar proportions among all four IgG subclasses during both the primary and secondary responses. The 44.1-Id usage was linked to the Igh(a), but not Igh(b), allotype and was associated with induction of relatively high total PPS14-specific IgG responses. In contrast to PPS14-protein conjugate, avidity maturation of the 44.1-Id-dominant PPS14-specific IgG responses was limited, even during the highly boosted T cell-dependent PPS14-specific secondary responses to COH1-11. These results indicate that different antigenic forms of the same capsular polysaccharide can recruit distinct B cell clones expressing characteristic idiotypes under genetic control and suggest that the 44.1-Id is derived from marginal zone B cells. | Animals, Antibodies, Monoclonal, Antigens, Bacterial, Bacterial Capsules, Binding Sites, Antibody, Female, Immunoglobulin Idiotypes, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, SCID, Pneumococcal Vaccines, Streptococcus pneumoniae, Vaccines, Conjugate | null |
22,706,081 | 2012-09-20 | 2013-11-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Exogenous administration of 15d-PGJ2-loaded nanocapsules inhibits bone resorption in a mouse periodontitis model. | Napimoga Marcelo H, da Silva Carlos A T, Carregaro Vanessa, Farnesi-de-Assunção Thais S, Duarte Poliana M, de Melo Nathalie F S, Fraceto Leonardo F | eng | null | Journal Article, Research Support, Non-U.S. Gov't | 15-deoxyprostaglandin J2, Anti-Inflammatory Agents, Non-Steroidal, Nanocapsules, Prostaglandin D2 | IM | 22706081, jimmunol.1200730, 10.4049/jimmunol.1200730 | The 15-deoxy-(Δ12,14)-PG J(2) (15d-PGJ(2)) has demonstrated excellent anti-inflammatory results in different experimental models. It can be used with a polymeric nanostructure system for modified drug release, which can change the therapeutic properties of the active principle, leading to increased stability and slower/prolonged release. The aim of the current study was to test a nanotechnological formulation as a carrier for 15d-PGJ(2), and to investigate the immunomodulatory effects of this formulation in a mouse periodontitis model. Poly (D,L-lactide-coglycolide) nanocapsules (NC) were used to encapsulate 15d-PGJ(2). BALB/c mice were infected on days 0, 2, and 4 with Aggregatibacter actinomycetemcomitans and divided into groups (n = 5) that were treated daily during 15 d with 1, 3, or 10 μg/kg 15d-PGJ(2)-NC. The animals were sacrificed, the submandibular lymph nodes were removed for FACS analysis, and the jaws were analyzed for bone resorption by morphometry. Immunoinflammatory markers in the gingival tissue were analyzed by reverse transcriptase-quantitative PCR, Western blotting, or ELISA. Infected animals treated with the 15d-PGJ(2)-NC presented lower bone resorption than infected animals without treatment (p < 0.05). Furthermore, infected animals treated with 10 μg/kg 15d-PGJ(2)-NC had a reduction of CD4(+)CD25(+)FOXP3(+) cells and CD4/CD8 ratio in the submandibular lymph node (p < 0.05). Moreover, CD55 was upregulated, whereas RANKL was downregulated in the gingival tissue of the 10 μg/kg treated group (p < 0.05). Several proinflammatory cytokines were decreased in the group treated with 10 μg/kg 15d-PGJ(2)-NC, and high amounts of 15d-PGJ(2) were observed in the gingiva. In conclusion, the 15d-PGJ(2)-NC formulation presented immunomodulatory effects, decreasing bone resorption and inflammatory responses in a periodontitis mouse model. | Actinobacillus Infections, Aggregatibacter actinomycetemcomitans, Animals, Anti-Inflammatory Agents, Non-Steroidal, Bone Resorption, Disease Models, Animal, Gingiva, Mice, Mice, Inbred BALB C, Mice, Inbred DBA, Nanocapsules, Periodontitis, Prostaglandin D2 | null |
22,706,083 | 2012-09-20 | 2012-07-09 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | The efficiency of human cytomegalovirus pp65(495-503) CD8+ T cell epitope generation is determined by the balanced activities of cytosolic and endoplasmic reticulum-resident peptidases. | Urban Sabrina, Textoris-Taube Kathrin, Reimann Barbara, Janek Katharina, Dannenberg Tanja, Ebstein Frédéric, Seifert Christin, Zhao Fang, Kessler Jan H, Halenius Anne, Henklein Petra, Paschke Julia, Cadel Sandrine, Bernhard Helga, Ossendorp Ferry, Foulon Thierry, Schadendorf Dirk, Paschen Annette, Seifert Ulrike | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Epitopes, T-Lymphocyte, Peptide Fragments, Peptide Hydrolases | IM | 22706083, jimmunol.1101886, 10.4049/jimmunol.1101886 | Control of human CMV (HCMV) infection depends on the cytotoxic activity of CD8(+) CTLs. The HCMV phosphoprotein (pp)65 is a major CTL target Ag and pp65(495-503) is an immunodominant CTL epitope in infected HLA-A*0201 individuals. As immunodominance is strongly determined by the surface abundance of the specific epitope, we asked for the components of the cellular Ag processing machinery determining the efficacy of pp65(495-503) generation, in particular, for the proteasome, cytosolic peptidases, and endoplasmic reticulum (ER)-resident peptidases. In vitro Ag processing experiments revealed that standard proteasomes and immunoproteasomes generate the minimal 9-mer peptide epitope as well as N-terminal elongated epitope precursors of different lengths. These peptides are largely degraded by the cytosolic peptidases leucine aminopeptidase and tripeptidyl peptidase II, as evidenced by increased pp65(495-503) epitope presentation after leucine aminopeptidase and tripeptidyl peptidase II knockdown. Additionally, with prolyl oligopeptidase and aminopeptidase B we identified two new Ag processing machinery components, which by destroying the pp65(495-503) epitope limit the availability of the specific peptide pool. In contrast to cytosolic peptidases, silencing of ER aminopeptidases 1 and 2 strongly impaired pp65(495-503)-specific T cell activation, indicating the importance of ER aminopeptidases in pp65(495-503) generation. Thus, cytosolic peptidases primarily interfere with the generation of the pp65(495-503) epitope, whereas ER-resident aminopeptidases enhance such generation. As a consequence, our experiments reveal that the combination of cytosolic and ER-resident peptidase activities strongly shape the pool of specific antigenic peptides and thus modulate MHC class I epitope presentation efficiency. | Antigen Presentation, CD8-Positive T-Lymphocytes, Cell Line, Cytomegalovirus Infections, Cytosol, Endoplasmic Reticulum, Epitopes, T-Lymphocyte, HeLa Cells, Humans, Peptide Fragments, Peptide Hydrolases | null |
22,706,085 | 2012-09-20 | 2012-07-09 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Defective macrophage migration in Gαi2- but not Gαi3-deficient mice. | Wiege Kristina, Le Duc D, Syed Shahzad N, Ali Syed R, Novakovic Ana, Beer-Hammer Sandra, Piekorz Roland P, Schmidt Reinhold E, Nürnberg Bernd, Gessner J Engelbert | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Lipopolysaccharides, Thioglycolates, GNAI3 protein, human, GTP-Binding Protein alpha Subunit, Gi2, GTP-Binding Protein alpha Subunits, Gi-Go, Gnai2 protein, mouse | IM | 22706085, jimmunol.1200891, 10.4049/jimmunol.1200891 | Various heterotrimeric G(i) proteins are considered to be involved in cell migration and effector function of immune cells. The underlying mechanisms, how they control the activation of myeloid effector cells, are not well understood. To elucidate isoform-redundant and -specific roles for Gα(i) proteins in these processes, we analyzed mice genetically deficient in Gα(i2) or Gα(i3). First, we show an altered distribution of tissue macrophages and blood monocytes in the absence of Gα(i2) but not Gα(i3). Gα(i2)-deficient but not wild-type or Gα(i3)-deficient mice exhibited reduced recruitment of macrophages in experimental models of thioglycollate-induced peritonitis and LPS-triggered lung injury. In contrast, genetic ablation of Gα(i2) had no effect on Gα(i)-dependent peritoneal cytokine production in vitro and the phagocytosis-promoting function of the Gα(i)-coupled C5a anaphylatoxin receptor by liver macrophages in vivo. Interestingly, actin rearrangement and CCL2- and C5a anaphylatoxin receptor-induced chemotaxis but not macrophage CCR2 and C5a anaphylatoxin receptor expression were reduced in the specific absence of Gα(i2). Furthermore, knockdown of Gα(i2) caused decreased cell migration and motility of RAW 264.7 cells, which was rescued by transfection of Gα(i2) but not Gα(i3). These results indicate that Gα(i2), albeit redundant to Gα(i3) in some macrophage activation processes, clearly exhibits a Gα(i) isoform-specific role in the regulation of macrophage migration. | Acute Lung Injury, Animals, Cell Migration Inhibition, GTP-Binding Protein alpha Subunit, Gi2, GTP-Binding Protein alpha Subunits, Gi-Go, Lipopolysaccharides, Macrophages, Mice, Mice, 129 Strain, Mice, Knockout, Monocytes, Peritonitis, Thioglycolates | null |
22,706,082 | 2012-09-20 | 2024-01-24 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Molecular mechanisms responsible for the selective and low-grade induction of proinflammatory mediators in murine macrophages by lipopolysaccharide. | Maitra Urmila, Deng Hui, Glaros Trevor, Baker Bianca, Capelluto Daniel G S, Li Zihai, Li Liwu | eng | R01 AI070603 (NIAID NIH HHS, United States); R01 HL115835 (NHLBI NIH HHS, United States); R01 AI064414 (NIAID NIH HHS, United States); R25 GM072767 (NIGMS NIH HHS, United States); AI070603 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Activating Transcription Factor 2, Atf2 protein, mouse, Inflammation Mediators, Intracellular Signaling Peptides and Proteins, Lipopolysaccharides, Phosphoinositide-3 Kinase Inhibitors, Reactive Oxygen Species, Tollip protein, mouse, Phosphatidylinositol 3-Kinase, Interleukin-1 Receptor-Associated Kinases | IM | 22706082, jimmunol.1200857, 10.4049/jimmunol.1200857, PMC3392521, NIHMS378901, 19794059, 10811644, 20530747, 20055671, 18256376, 16428431, 9575168, 18029230, 18305141, 19575462, 11733770, 17876711, 20037584, 12055225, 12509509, 18669610, 12736252, 17531663, 15140579, 19289601, 17972350, 8556521, 12433365, 16412388, 16750842, 21145432, 17203472, 11751856, 15211360, 19270711, 21531375, 8641750, 10854325, 21068409, 19656901, 21525932, 19556980, 15474523, 15268749, 21098227, 18981139, 19712049, 20684772, 18575590, 10588212, 19325485, 8426119, 12391239, 21930231, 12150927, 11900166, 1815338, 20007531, 8388859, 15069085, 20447537, 16951372, 18240558, 17113392, 21676485, 20584979, 21597473, 19949414, 22143158, 11402340, 16613850, 21175546, 20489162, 16211093, 20639876, 21636801, 17536046, 21282379, 20865800, 19690169, 20303729, 19699171, 22190366, 21825134, 3881930, 20592272, 6192184, 19840932, 9558074, 19020113, 9734363, 21255011, 16007092, 18458086, 17275323, 21357541 | Low-dose endotoxemia is prevalent in humans with adverse health conditions, and it correlates with the pathogenesis of chronic inflammatory diseases such as atherosclerosis, diabetes, and neurologic inflammation. However, the underlying molecular mechanisms are poorly understood. In this study, we demonstrate that subclinical low-dose LPS skews macrophages into a mild proinflammatory state, through cell surface TLR4, IL-1R-associated kinase-1, and the Toll-interacting protein. Unlike high-dose LPS, low-dose LPS does not induce robust activation of NF-κB, MAPKs, PI3K, or anti-inflammatory mediators. Instead, low-dose LPS induces activating transcription factor 2 through Toll-interacting protein-mediated generation of mitochondrial reactive oxygen species, allowing mild induction of proinflammatory mediators. Low-dose LPS also suppresses PI3K and related negative regulators of inflammatory genes. Our data reveal novel mechanisms responsible for skewed and persistent low-grade inflammation, a cardinal feature of chronic inflammatory diseases. | Activating Transcription Factor 2, Animals, Bone Marrow Cells, Cells, Cultured, Dose-Response Relationship, Immunologic, Gene Expression Regulation, Inflammation Mediators, Interleukin-1 Receptor-Associated Kinases, Intracellular Fluid, Intracellular Signaling Peptides and Proteins, Lipopolysaccharides, Macrophages, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria, Phosphatidylinositol 3-Kinase, Phosphoinositide-3 Kinase Inhibitors, Reactive Oxygen Species, Signal Transduction | null |
22,706,087 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Cutting edge: mast cells critically augment myeloid-derived suppressor cell activity. | Saleem Sheinei J, Martin Rebecca K, Morales Johanna K, Sturgill Jamie L, Gibb David R, Graham Laura, Bear Harry D, Manjili Masoud H, Ryan John J, Conrad Daniel H | eng | U19 AI077435 (NIAID NIH HHS, United States); F31CA159877 (NCI NIH HHS, United States); U19AI077435 (NIAID NIH HHS, United States); R01AI19697 (NIAID NIH HHS, United States); F31 CA159877 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22706087, jimmunol.1200647, 10.4049/jimmunol.1200647, PMC3392490, NIHMS380790, 2455691, 9762559, 19336265, 19553533, 20171075, 9181234, 21061431, 21949025, 20414655, 19198835, 21041732, 19197294, 19265258, 21314739, 20111717, 18505479, 18432905, 21368228, 21085745, 21216893, 18524989, 6687492, 17255288, 11090068, 19483649, 8988878 | Myeloid-derived suppressor cells (MDSCs) are primarily recognized for their immunosuppressive properties in malignant disease. However, their interaction with other innate immune cells and their regulation of immune responses, such as in parasitic infection, necessitate further characterization. We used our previously published mouse model of MDSC accumulation to examine the immunoregulatory role of MDSCs in B16 melanoma metastasis and Nippostrongylus brasiliensis infection. In this study, we demonstrate that the activity of MDSCs is dependent on the immune stimuli and subset induced. Monocytic MDSCs predictably suppressed antitumor immune responses but granulocytic MDSCs surprisingly enhanced the clearance of N. brasiliensis infection. Intriguingly, both results were dependent on MDSC interaction with mast cells (MCs), as demonstrated by adoptive-transfer studies in MC-deficient (Kit(Wsh)(/)(Wsh)) mice. These findings were further supported by ex vivo cocultures of MCs and MDSCs, indicating a synergistic increase in cytokine production. Thus, MCs can enhance both immunosuppressive and immunosupportive functions of MDSCs. | Animals, Carcinoma, Lewis Lung, Cell Communication, Cell Line, Tumor, Cells, Cultured, Coculture Techniques, Granulocytes, Mast Cells, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, Monocytes, Myeloid Cells, Nippostrongylus | null |
22,706,086 | 2012-09-20 | 2021-10-21 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | CD2AP/SHIP1 complex positively regulates plasmacytoid dendritic cell receptor signaling by inhibiting the E3 ubiquitin ligase Cbl. | Bao Musheng, Hanabuchi Shino, Facchinetti Valeria, Du Qiumei, Bover Laura, Plumas Joel, Chaperot Laurence, Cao Wei, Qin Jun, Sun Shao-Cong, Liu Yong-Jun | eng | P30 CA016672 (NCI NIH HHS, United States); CA016672 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Adaptor Proteins, Signal Transducing, CD2-associated protein, CLEC4C protein, human, Cross-Linking Reagents, Cytoskeletal Proteins, Lectins, C-Type, Membrane Glycoproteins, Multiprotein Complexes, Receptors, Immunologic, Proto-Oncogene Proteins c-cbl, Phosphoric Monoester Hydrolases, Inositol Polyphosphate 5-Phosphatases, INPP5D protein, human, Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases, CBL protein, human | IM | 22706086, jimmunol.1200887, 10.4049/jimmunol.1200887, PMC3665352, NIHMS464335, 17850179, 11067845, 15771572, 11742985, 15229652, 19564354, 20457601, 11894095, 11748283, 19587372, 21372129, 16735691, 18218851, 21155837, 17675467, 16365416, 20404851, 8910587, 10364556, 11894096, 15511676, 16973389, 18022864, 18034431, 12354621, 12960479, 15001553, 20484116, 12145291 | The human plasmacytoid dendritic cell (pDC) receptor BDCA2 forms a complex with the adaptor FcεR1γ to activate an ITAM-signaling cascade. BDCA2 receptor signaling negatively regulates the TLR7/9-mediated type 1 IFN responses in pDCs, which may play a key role in controlling self-DNA/RNA-induced autoimmunity. We report in this article that CD2-associated adaptor protein (CD2AP), which is highly expressed in human pDCs, positively regulates BDCA2/FcεR1γ receptor signaling. By immunoprecipitation and mass spectrometry analyses, we found that CD2AP bound to SHIP1. Knockdown of CD2AP or SHIP1 reduced the BDCA2/FcεR1γ-mediated ITAM signaling and blocked its inhibition of TLR9-mediated type 1 IFN production. Knockdown of CD2AP or SHIP1 also enhanced the ubiquitination and degradation of Syk and FcεR1γ that was mediated by the E3 ubiquitin ligase Cbl. This led us to discover that, upon BDCA2 cross-linking, the CD2AP/SHIP1 complex associated with Cbl and inhibited its E3 ubiquitin ligase activity. In human primary pDCs, cross-linking of the BDCA2/FcεR1γ complex induced the recruitment of the CD2AP/SHIP1/Cbl complex to the plasma membrane of pDCs, where it colocalized with the BDCA2/FcεR1γ complex. Therefore, CD2AP positively regulates BDCA2/FcεR1γ signaling by forming a complex with SHIP1 to inhibit the E3 ubiquitin ligase Cbl. | Adaptor Proteins, Signal Transducing, Cells, Cultured, Cross-Linking Reagents, Cytoskeletal Proteins, Dendritic Cells, HEK293 Cells, Humans, Inositol Polyphosphate 5-Phosphatases, Lectins, C-Type, Membrane Glycoproteins, Multiprotein Complexes, Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases, Phosphoric Monoester Hydrolases, Proto-Oncogene Proteins c-cbl, Receptors, Immunologic, Signal Transduction, Up-Regulation | null |
22,706,084 | 2012-09-20 | 2024-06-10 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Analysis of T cell responses to the major allergens from German cockroach: epitope specificity and relationship to IgE production. | Oseroff Carla, Sidney John, Tripple Victoria, Grey Howard, Wood Robert, Broide David H, Greenbaum Jason, Kolla Ravi, Peters Bjoern, Pomés Anna, Sette Alessandro | eng | N01-AI-25482 (NIAID NIH HHS, United States); N01 AI040023 (NIAID NIH HHS, United States); U19 AI100275 (NIAID NIH HHS, United States); R37 AI038425 (NIAID NIH HHS, United States); N01 AI025482 (NIAID NIH HHS, United States); HHSN272200700048C (NIAID NIH HHS, United States); R01AI077653 (NIAID NIH HHS, United States); HSN272200700048C (PHS HHS, United States); U19AI100275 (NIAID NIH HHS, United States); N01AI25482 (NIAID NIH HHS, United States); HHSN272200900052C (NIAID NIH HHS, United States); R01 AI077653 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Allergens, Bla g 5 allergen, Blattella germanica, Bla g 6 allergen, Blattella germanica, Bla g 7 allergen, Blattella germanica, Blatella germanica allergen 4, Epitopes, T-Lymphocyte, HLA-DP Antigens, HLA-DQ Antigens, HLA-DR Antigens, Insect Proteins, Tropomyosin, allergen Bla g 1, Immunoglobulin E, Aspartic Acid Endopeptidases, allergen Bla g 2 | IM | 22706084, jimmunol.1200694, 10.4049/jimmunol.1200694, PMC3392449, NIHMS380404, 12753660, 18490718, 20019801, 11149990, 2715548, 4202581, 10201967, 18432745, 18081934, 7642642, 11149989, 20951144, 10669532, 21092157, 2460431, 9150410, 15806002, 17548627, 21305276, 18245380, 18842709, 15173208, 20810981, 19638038, 17703094, 16767078, 12373266, 19489919, 15753892, 17418661, 9252418, 16751002, 9134876, 9804858, 18251694, 21530813, 20139279, 19149776, 17617598, 17581196, 17513729, 9678832, 12731045, 12534547, 8872483, 20554959, 16172378, 7613153, 18508645, 10808174, 19001794, 20565291, 8537384, 10848915, 17200302, 16912286, 21323911, 12562550, 1591932, 11140401, 20604801, 11240940 | Bla g allergens are major targets of IgE responses associated with cockroach allergies. However, little is known about corresponding T cell responses, despite their potential involvement in immunopathology and the clinical efficacy of specific immunotherapy. Bioinformatic predictions of the capacity of Bla g 1, 2, 4, 5, 6, and 7 peptides to bind HLA-DR, -DP, and -DQ molecules, and PBMC responses from 30 allergic donors, identified 25 T cell epitopes. Five immunodominant epitopes accounted for more than half of the response. Bla g 5, the most dominant allergen, accounted for 65% of the response, and Bla g 6 accounted for 20%. Bla g 5 induced both IL-5 and IFN-γ responses, whereas Bla g 6 induced mostly IL-5, and, conversely, Bla g 2 induced only IFN-γ. Thus, responses to allergens within a source are independently regulated, suggesting a critical role for the allergen itself, and not extraneous stimulation from other allergens or copresented immunomodulators. In comparing Ab with T cell responses for several donor/allergen combinations, we detected IgE titers in the absence of detectable T cell responses, suggesting that unlinked T cell-B cell help might support development of IgE responses. Finally, specific immunotherapy resulted in IL-5 down modulation, which was not associated with development of IFN-γ or IL-10 responses to any of the Bla g-derived peptides. In summary, the characteristics of T cell responses to Bla g allergens appear uncorrelated with IgE responses. Monitoring these responses may therefore yield important information relevant to understanding cockroach allergies and their treatment. | Allergens, Amino Acid Sequence, Animals, Aspartic Acid Endopeptidases, Cells, Cultured, Epitopes, T-Lymphocyte, HLA-DP Antigens, HLA-DQ Antigens, HLA-DR Antigens, Humans, Immunoglobulin E, Insect Proteins, Molecular Sequence Data, Protein Binding, T-Lymphocyte Subsets, Tropomyosin | null |
22,706,090 | 2013-03-25 | 2014-11-20 | 1528-1159 | Spine | Annulus fissures are mechanically and chemically conducive to the ingrowth of nerves and blood vessels. | Stefanakis Manos, Al-Abbasi Maan, Harding Ian, Pollintine Phillip, Dolan Patricia, Tarlton John, Adams Michael A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Phenazines, Proteoglycans, safranine T | IM | 22706090, 10.1097/BRS.0b013e318263ba59 | Mechanical and biochemical analyses of cadaveric and surgically removed discs. | Adolescent, Adult, Aged, Aged, 80 and over, Blood Vessels, Cadaver, Cell Movement, Child, Female, Humans, In Vitro Techniques, Intervertebral Disc, Intervertebral Disc Degeneration, Male, Middle Aged, Phenazines, Proteoglycans, Spinal Nerves, Stress, Mechanical, Young Adult | null |
22,706,088 | 2012-09-20 | 2024-10-01 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Tim-3 pathway controls regulatory and effector T cell balance during hepatitis C virus infection. | Moorman Jonathan P, Wang Jia M, Zhang Ying, Ji Xiao J, Ma Cheng J, Wu Xiao Y, Jia Zhan S, Wang Ke S, Yao Zhi Q | eng | R01 DK093526 (NIDDK NIH HHS, United States); R15 AI084057 (NIAID NIH HHS, United States); R01DK093526 (NIDDK NIH HHS, United States); R15A1084057 (PHS HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Apoptosis Regulatory Proteins, FOXP3 protein, human, Forkhead Transcription Factors, Interleukin-2 Receptor alpha Subunit, Receptors, Immunologic, TIGIT protein, human | IM | 22706088, jimmunol.1200162, 10.4049/jimmunol.1200162, PMC3392408, NIHMS378649, 21376795, 20209097, 21844165, 16670334, 15785759, 15163734, 18397267, 21091911, 21263070, 21079629, 20049876, 15307180, 15827965, 20883163, 20195282, 11323285, 17603844, 19144287, 15896245, 12093005, 11441082, 11792059, 14517080, 21040831, 21084749, 11673541, 21291295, 19234217, 28115586, 19464986, 17220874, 11984145, 20975732, 10613751, 19015312, 17948021, 20623550, 17982458, 21122941, 21637332, 15699103, 16206250, 16122679, 15894587, 21446887, 11086025, 11466340, 15684039, 19229109, 19517231 | Hepatitis C virus (HCV) is remarkable at disrupting human immunity to establish chronic infection. Upregulation of inhibitory signaling pathways (such as T cell Ig and mucin domain protein-3 [Tim-3]) and accumulation of regulatory T cells (Tregs) play pivotal roles in suppressing antiviral effector T cell (Teff) responses that are essential for viral clearance. Although the Tim-3 pathway has been shown to negatively regulate Teffs, its role in regulating Foxp3(+) Tregs is poorly explored. In this study, we investigated whether and how the Tim-3 pathway alters Foxp3(+) Treg development and function in patients with chronic HCV infection. We found that Tim-3 was upregulated, not only on IL-2-producing CD4(+)CD25(+)Foxp3(-) Teffs, but also on CD4(+)CD25(+)Foxp3(+) Tregs, which accumulate in the peripheral blood of chronically HCV-infected individuals when compared with healthy subjects. Tim-3 expression on Foxp3(+) Tregs positively correlated with expression of the proliferation marker Ki67 on Tregs, but it was inversely associated with proliferation of IL-2-producing Teffs. Moreover, Foxp3(+) Tregs were found to be more resistant to, and Foxp3(-) Teffs more sensitive to, TCR activation-induced cell apoptosis, which was reversible by blocking Tim-3 signaling. Consistent with its role in T cell proliferation and apoptosis, blockade of Tim-3 on CD4(+)CD25(+) T cells promoted expansion of Teffs more substantially than Tregs through improving STAT-5 signaling, thus correcting the imbalance of Foxp3(+) Tregs/Foxp3(-) Teffs that was induced by HCV infection. Taken together, the Tim-3 pathway appears to control Treg and Teff balance through altering cell proliferation and apoptosis during HCV infection. | Apoptosis Regulatory Proteins, Cell Proliferation, Forkhead Transcription Factors, Hepatitis C, Chronic, Humans, Interleukin-2 Receptor alpha Subunit, Pilot Projects, Receptors, Immunologic, Signal Transduction, T-Lymphocyte Subsets, T-Lymphocytes, Regulatory, Viremia | null |
22,706,089 | 2012-09-20 | 2025-01-03 | 1550-6606 | Journal of immunology (Baltimore, Md. : 1950) | Autocrine IFN-γ promotes naive CD8 T cell differentiation and synergizes with IFN-α to stimulate strong function. | Curtsinger Julie M, Agarwal Pujya, Lins Debra C, Mescher Matthew F | eng | P01 AI035296 (NIAID NIH HHS, United States); R01 AI034824 (NIAID NIH HHS, United States); AI35296 (NIAID NIH HHS, United States); AI34824 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Interferon-alpha, T-Box Domain Proteins, T-bet Transcription Factor, Interleukin-12, Interferon-gamma | IM | 22706089, jimmunol.1102727, 10.4049/jimmunol.1102727, PMC3392455, NIHMS378898, 10092777, 17513722, 15814665, 19359475, 18818389, 19833085, 15516969, 17675465, 12172544, 11786644, 20164422, 14605368, 9218573, 14983028, 20363604, 10452994, 8287475, 17675470, 15240678, 16585541, 19592655, 17981204, 2573841, 17723218, 16273099, 14673093, 16824119, 14607916, 19409816, 16849484, 9860975, 11313382, 17698591, 19234173, 20393139, 15585842, 15905533, 15905520, 11752460, 11854360, 17617612, 15809350, 12006974, 16129706 | Autocrine IFN-γ signaling is important for CD4 differentiation to Th1 effector cells, but it has been unclear whether it contributes to CD8 T cell differentiation. We show in this paper that naive murine CD8 T cells rapidly and transiently produce low levels of IFN-γ upon stimulation with Ag and B7-1, with production peaking at ∼8 h and declining by 24 h. The autocrine IFN-γ signals for upregulation of expression of T-bet and granzyme B and induces weak cytolytic activity and effector IFN-γ production. IFN-α acts synergistically with IFN-γ to support development of strong effector functions, whereas IL-12 induces high T-bet expression and strong function in the absence of IFN-γ signaling. Thus, IFN-γ is not only an important CD8 T cell effector cytokine, it is an autocrine/paracrine factor whose contributions to differentiation vary depending on whether the response is supported by IL-12 or type I IFN. | Adoptive Transfer, Animals, Autocrine Communication, CD8-Positive T-Lymphocytes, Cell Differentiation, Cell Line, Tumor, Cells, Cultured, Interferon-alpha, Interferon-gamma, Interleukin-12, Melanoma, Experimental, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Paracrine Communication, T-Box Domain Proteins, Up-Regulation, T-bet Transcription Factor | null |
22,706,091 | 2013-05-22 | 2022-03-21 | 1528-1159 | Spine | Adjacent level degeneration and facet arthropathy after disc prosthesis surgery or rehabilitation in patients with chronic low back pain and degenerative disc: second report of a randomized study. | Hellum Christian, Berg Linda, Gjertsen Øivind, Johnsen Lars Gunnar, Neckelmann Gesche, Storheim Kjersti, Keller Anne, Grundnes Oliver, Espeland Ansgar | eng | null | Comparative Study, Journal Article, Multicenter Study, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | null | IM | 22706091, 10.1097/BRS.0b013e318263cc46 | Randomized clinical trial with 2-year follow-up. | Adult, Chronic Pain, Disability Evaluation, Female, Humans, Intervertebral Disc, Intervertebral Disc Degeneration, Low Back Pain, Lumbar Vertebrae, Magnetic Resonance Imaging, Male, Middle Aged, Norway, Pain Measurement, Postoperative Complications, Predictive Value of Tests, Sacrum, Time Factors, Total Disc Replacement, Treatment Outcome, Zygapophyseal Joint | null |
22,706,093 | 2013-05-23 | 2019-12-10 | 1879-2782 | Neural networks : the official journal of the International Neural Network Society | Online learning and generalization of parts-based image representations by non-negative sparse autoencoders. | Lemme Andre, Reinhart René Felix, Steil Jochen Jakob | eng | null | Journal Article | null | IM | 22706093, S0893-6080(12)00145-1, 10.1016/j.neunet.2012.05.003 | We present an efficient online learning scheme for non-negative sparse coding in autoencoder neural networks. It comprises a novel synaptic decay rule that ensures non-negative weights in combination with an intrinsic self-adaptation rule that optimizes sparseness of the non-negative encoding. We show that non-negativity constrains the space of solutions such that overfitting is prevented and very similar encodings are found irrespective of the network initialization and size. We benchmark the novel method on real-world datasets of handwritten digits and faces. The autoencoder yields higher sparseness and lower reconstruction errors than related offline algorithms based on matrix factorization. It generalizes to new inputs both accurately and without costly computations, which is fundamentally different from the classical matrix factorization approaches. | Artificial Intelligence, Databases, Factual, Face, Humans, Image Processing, Computer-Assisted, Neural Networks, Computer, Pattern Recognition, Automated, Photic Stimulation | null |
22,706,092 | 2013-05-23 | 2012-07-17 | 1879-2782 | Neural networks : the official journal of the International Neural Network Society | A meta-learning approach to the regularized learning-case study: blood glucose prediction. | Naumova V, Pereverzyev S V, Sivananthan S | eng | null | Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Blood Glucose | IM | 22706092, S0893-6080(12)00146-3, 10.1016/j.neunet.2012.05.004 | In this paper we present a new scheme of a kernel-based regularization learning algorithm, in which the kernel and the regularization parameter are adaptively chosen on the base of previous experience with similar learning tasks. The construction of such a scheme is motivated by the problem of prediction of the blood glucose levels of diabetic patients. We describe how the proposed scheme can be used for this problem and report the results of the tests with real clinical data as well as comparing them with existing literature. | Adolescent, Adult, Aged, Algorithms, Blood Glucose, Case-Control Studies, Diabetes Mellitus, Female, Humans, Learning, Male, Middle Aged, Predictive Value of Tests, Young Adult | null |
22,706,098 | 2013-04-18 | 2012-09-24 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Bis (trifluoromethyl) sulfone, CF3SO2CF3: synthesis, vibrational and conformational properties. | Defonsi Lestard M E, Ramos L A, Tuttolomondo M E, Ulic S E, Ben Altabef A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Sulfones | IM | 22706098, S1386-1425(12)00505-7, 10.1016/j.saa.2012.05.049 | Bis (trifluoromethyl) sulfone, CF(3)SO(2)CF(3), was obtained as a byproduct in the synthesis of CF(3)SO(2)SCF(3). The compound was characterized by infrared and Raman spectroscopy as well quantum chemical calculations. Quantum mechanical calculations indicate the possible existence of two conformers symmetrically equivalent with C(2) symmetry. The preference for the staggered form was studied using the total energy scheme and the natural bond orbital (NBO) partition scheme. Additionally, the total potential energy was deconvoluted using a sixfold decomposition in terms of a Fourier-type expansion, showing that the hyperconjugative effect was dominant in stabilizing the staggered conformer. Infrared and Raman spectra of CF(3)SO(2)CF(3) were obtained. Harmonic vibrational wavenumbers and a scaled force field were calculated, leading to a final root mean-square deviation of 7.8 cm(-1) when comparing experimental and calculated wavenumbers. | Kinetics, Models, Molecular, Molecular Conformation, Quantum Theory, Spectrophotometry, Infrared, Spectrum Analysis, Raman, Sulfones, Thermodynamics, Torsion, Mechanical, Vibration | null |
22,706,097 | 2013-04-18 | 2025-01-03 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Study of the interaction between bovine serum albumin and analogs of Biphenyldicarboxylate by spectrofluorimetry. | Wang Ruiyong, Chai Yahui, Wang Ruiqiang, Zhang Lu, Wu Jing, Chang Junbiao | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biphenyl Compounds, Serum Albumin, Bovine, biphenyl | IM | 22706097, S1386-1425(12)00486-6, 10.1016/j.saa.2012.05.030 | This work was designed to study the interaction between bovine serum albumin (BSA) and Biphenyldicarboxylate (DDB) or analogs (I, II and III) of DDB by UV-visible and fluorescence spectroscopic methods for the first time. Results showed that both DDB and analogs had a strong ability to quench the intrinsic fluorescence of BSA through a static quenching procedure. The binding constants (K) were calculated according to the relevant fluorescence data. The thermodynamic parameters (ΔH, ΔS and ΔG) showed that the hydrophobic force played a major role in the binding interaction between BSA and DDB or analogs. Synchronous fluorescence spectra of BSA were investigated in the presence of DDB or analogs. It was showed that DDB was the strongest quencher and bound to BSA with the highest affinity among four compounds. The influence of molecular structure on the binding aspects was reported. | Animals, Biphenyl Compounds, Cattle, Energy Transfer, Fluorometry, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Protein Binding, Serum Albumin, Bovine, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Thermodynamics | null |
22,706,099 | 2013-04-18 | 2016-11-25 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Tautomeric purine forms of 2-amino-6-chloropurine (N9H10 and N7H10): structures, vibrational assignments, NBO analysis, hyperpolarizability, HOMO-LUMO study using B3 based density functional calculations. | Balachandran V, Parimala K | eng | null | Journal Article | 2-Aminopurine, 2-amino-6-chloropurine | IM | 22706099, S1386-1425(12)00506-9, 10.1016/j.saa.2012.05.050 | Two purine tautomers of 2-amino-6-chloropurine (ACP), in labeled as N(9)H(10) and N(7)H(10), were investigated by vibrational spectroscopy and quantum chemical method. The FT-IR and FT-Raman spectra of ACP have been recorded in the regions 4000-400 cm(-1) and 3500-100 cm(-1), respectively. The measured spectra were interpreted by aid of a normal coordinate analysis following DFT full geometry optimization and vibrational frequency calculations at B3LYP/6-311++G(d,p) level. First-order hyperpolarizability, HOMO and LUMO energies were calculated at same level of theory. The calculated molecular geometry has been compared with the X-ray data. The observed and calculated frequencies were found in good agreement. The obtained NBO data and second-order perturbation energy values to elucidate the Lewis and non-Lewis types of bonding structures in the purine tautomer N(9)H(10), have indicated the presence of an intramolecular hyperconjucative interaction between lone pair N and N-C bond orbital. | 2-Aminopurine, Models, Molecular, Molecular Conformation, Quantum Theory, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman, Stereoisomerism, Thermodynamics, Vibration | null |
22,706,100 | 2013-04-18 | 2013-11-21 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Spectral studies on the interaction between Cu2+ and urease. | Yan-Qing Wang, Hong-Mei Zhang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Copper, Urease | IM | 22706100, S1386-1425(12)00470-2, 10.1016/j.saa.2012.05.014 | The interactions of Cu(2+) with urease were investigated by fluorescence, UV/vis, CD, synchronous fluorescence, and three-dimensional fluorescence spectra techniques. Cu(2+) effectively quenched the intrinsic fluorescence of urease via static quenching. The binding constant K(A), the binding site n and the thermodynamic parameters are obtained. The process of binding Cu(2+) to urease was a spontaneous molecular interaction procedure with electrostatic interaction. The conformation of urease was discussed by UV/vis, CD, synchronous and three-dimensional fluorescence techniques. | Absorption, Circular Dichroism, Copper, Hydrogen-Ion Concentration, Kinetics, Molecular Conformation, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Temperature, Urease | null |
22,706,095 | 2013-02-05 | 2021-10-21 | 1096-3650 | Seminars in cancer biology | Linking epithelial-to-mesenchymal-transition and epigenetic modifications. | Stadler Sonja C, Allis C David | eng | R01 GM098870 (NIGMS NIH HHS, United States); R01GM098870 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Antineoplastic Agents, Histones, MicroRNAs | IM | 22706095, S1044-579X(12)00099-5, 10.1016/j.semcancer.2012.06.007, PMC3445725, NIHMS391323, 17822958, 15215868, 19008416, 21941284, 17898713, 17320507, 10819507, 17012846, 20504901, 16882971, 12042769, 21447744, 21889194, 19625769, 20696752, 14612500, 18349321, 15925505, 17508028, 20562920, 19372393, 15164071, 16401708, 11498575, 20592490, 15454082, 9620804, 19239879, 19572217, 21272588, 22015296, 21252315, 21874049, 11891299, 21317364, 10368776, 9620779, 17804704, 18600261, 15608514, 15793220, 18270520, 17603471, 17296563, 16493418, 11352938, 19826124, 20676129, 17822959, 16638127, 22000009, 10944586, 17968323, 17437993, 12953102, 17463086, 19881528, 19752007, 21740232, 6185846, 19177007, 18829489, 12171503, 21074720, 21949397, 17761946, 17320506, 14744436, 21814200, 18381893, 17890317, 19258506, 19934284, 21983900, 17320500, 19372391, 21725293, 22170610, 18392623, 12923528, 15211354, 12702868, 20871596, 16369569, 12189386, 15944708, 6197868, 18232738, 20473948, 19945376, 14990586, 16485345, 21719641, 19158989, 2579435, 20651253, 10638745, 19072646, 20944599, 18193036, 21770894, 10647931, 12021783, 18719391, 14764558, 19339683, 18376396, 11250729, 3409869, 20682048, 18185580, 11156387, 17948228, 20084174, 18411277, 22286061, 18211683, 9305837, 7573028, 18768788, 22187223, 17339880, 19262571, 20211137, 12508111, 10458604, 20531367, 21460836, 20574448, 21490601, 11782440, 21277012, 15123803 | Cancer, as well as other human disorders, has long been considered to result from the consequence of genetic mutations in key regulatory genes that reside in pathways controlling proliferation, cellular differentiation, DNA damage and repair. In the case of cancer, mutations are well documented to arise in key oncogenes and critically important tumor-suppressor genes as part of the disease progression process. In addition to more accepted, genetic mutations, a rapidly increasing body of evidence supports the general view that profound alterations also occur in 'epigenes', whose products serve to define the 'epigenetic landscape' of tumor cells. Aberrant changes in epigenetic mechanisms such as DNA methylation, histone modifications and expression of micro RNAs play an important role in cancer and contribute to malignant transitions. Here we review recent studies linking epigenetic mechanisms to epithelial-to-mesenchymal transition as defined in normal processes, as well as abnormal transitions that lead to oncogensis. | Antineoplastic Agents, DNA Methylation, Epigenesis, Genetic, Epithelial-Mesenchymal Transition, Gene Expression Regulation, Neoplastic, Histones, Humans, MicroRNAs, Neoplasms | null |
22,706,101 | 2013-04-18 | 2022-04-09 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Aluminium sensitized spectrofluorimetric determination of fluoroquinolones in milk samples coupled with salting-out assisted liquid-liquid ultrasonic extraction. | Xia Qinghai, Yang Yaling, Liu Mousheng | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acetonitriles, Fluoroquinolones, Solutions, Water, Ciprofloxacin, Ofloxacin, Aluminum, Gatifloxacin, Norfloxacin, Ammonium Sulfate, acetonitrile | IM | 22706101, S1386-1425(12)00504-5, 10.1016/j.saa.2012.05.048 | An aluminium sensitized spectrofluorimetric method coupled with salting-out assisted liquid-liquid ultrasonic extraction for the determination of four widely used fluoroquinolones (FQs) namely norfloxacin (NOR), ofloxacin (OFL), ciprofloxacin (CIP) and gatifloxacin (GAT) in bovine raw milk was described. The analytical procedure involves the fluorescence sensitization of aluminium (Al(3+)) by complexation with FQs, salting-out assisted liquid-liquid ultrasonic extraction (SALLUE), followed by spectrofluorometry. The influence of several parameters on the extraction (the salt species, the amount of salt, pH, temperature and phase volume ratio) was investigated. Under optimized experimental conditions, the detection limits of the method in milk varied from 0.009 μg/mL for NOR to 0.016 μg/mL for GAT (signal-to-noise ratio (S/N)=3). The relative standard deviations (RSD) values were found to be relatively low (0.54-2.48% for four compounds). The calibration graph was linear from 0.015 to 2.25 μg/mL with coefficient of determinations not less than 0.9974. The methodology developed was applied to the determination of FQs in bovine raw milk samples. The main advantage of this method is simple, accurate and green. The method showed promising applications for analyzing polar analytes especially polar drugs in various sample matrices. | Acetonitriles, Aluminum, Ammonium Sulfate, Animals, Cattle, Ciprofloxacin, Fluoroquinolones, Gatifloxacin, Hydrogen-Ion Concentration, Limit of Detection, Liquid-Liquid Extraction, Milk, Norfloxacin, Ofloxacin, Solutions, Spectrometry, Fluorescence, Temperature, Ultrasonics, Water | null |
22,706,103 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [The role of proteins in neurodegenerative disease]. | Szwed Aleksandra, Miłowska Katarzyna | pol | null | Journal Article, Review | Amyloid beta-Peptides, Prions, alpha-Synuclein, tau Proteins | IM | 22706103, 991446, 10.5604/17322693.991446 | All neurodegenerative diseases are related to pathology and accumulation of proteins. Proteins are basic structural and functional components of each cell and their functions are associated with their amino acid composition and spatial structure. The proper functioning of protein is necessary for the proper operation of the body system. In the case of disorders of proteins' spatial structure, the development of pathological processes may occur. Accumulation of abnormal proteins is toxic to nerve cells and causes neurodegeneration. Different disorders are characterized by abnormalities of various proteins. This type of neurodegenerative diseases includes Parkinson's disease, tauopathies, Alzheimer's disease, and prion diseases. Parkinson's disease is characterized by toxicity of α-synuclein. The pathology of tau protein is specific for tauopathies, prion protein for prion diseases. In the case of Alzheimer's disease it is β-amyloid. All proteins responsible for the pathology are present in the physiological state in the organism. Damage to the area of the brain covered by the pathological process and the clinical symptoms are characteristic for a particular type of disease. Detailed knowledge of the mechanisms of the disease can be an important element in the development of effective ways of treatment. | Alzheimer Disease, Amyloid beta-Peptides, Brain, Humans, Molecular Structure, Neurodegenerative Diseases, Neurons, Parkinson Disease, Prion Diseases, Prions, Synaptic Transmission, Tauopathies, alpha-Synuclein, tau Proteins | null |
22,706,102 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Hair follicle as a novel source of stem cells]. | Joachimiak Romana, Bajek Anna, Drewa Tomasz | pol | null | Journal Article, Review | null | IM | 22706102, 991445, 10.5604/17322693.991445 | Tissue engineering as a rapidly developing branch of science offers hope for the use of its products in medical practice. Among the components of tissue substitutes are different types of cells, especially stem cells. A promising source of adult stem cells is hair follicles. Development of follicles in the skin takes place even during fetal life. They arise due to the impact of epidermal and mesenchymal cells. The next steps in the formation of hair follicles are under the control of many factors. Hair follicles are the niche of various stem cell populations and are a major source of cells responsible for regeneration of the hair, sebaceous glands and epidermis. The term "hair follicle stem cells" is most often used in relation to the epithelial cell population. Hair follicle stem cell studies are complicated by the fact that these stem cells divide relatively rarely. The aim of this study is to present the characteristics of cells isolated from the hair follicle in the light of recent research. | Animals, Epidermal Cells, Epithelial Cells, Hair Follicle, Humans, Regeneration, Sebaceous Glands, Skin, Stem Cells, Tissue Engineering | null |
22,706,104 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [The discovery of neuromedin U and its pivotal role in the central regulation of energy homeostasis]. | Kirsz Katarzyna, Zięba Dorota A | pol | null | Journal Article, Review | Calcium Channels, L-Type, Neuropeptides, Receptors, Neurotransmitter, neuromedin S, neuromedin U receptor, neuromedin U, Mitogen-Activated Protein Kinases, Type C Phospholipases, GTP-Binding Protein alpha Subunits, Gq-G11 | IM | 22706104, 991448, 10.5604/17322693.991448 | Neuromedin U (NMU) is a structurally highly conserved neuropeptide and has been paired with the G-protein-coupled receptors (GPCRs) NMUR1 and NMUR2, which were formerly classified in the orphan receptor family. Activation of the G protein Gq/11 subunit causes a pertussis toxin (PTX)-insensitive activation of both phospholipase C and mitogen-activated protein kinase (MAP), and activation of the Go subunit causes a PTX-sensitive inhibition of adenyl cyclase. Additionally, NMU selectively inhibits L-type high-voltage-gated Ca2+ channels in mouse hippocampus, as well as low-voltage-activated T-type Ca2+ channels in mouse dorsal root ganglia (DRG). NMU peptide and its receptors are predominantly expressed in the gastrointestinal tract and specific structures within the brain, reflecting its major role in the regulation of energy homeostasis. A novel neuropeptide, neuromedin S (NMS), is structurally related to NMU. They share a C-terminal core structure and both have been implicated in the regulation of food intake, as well as the circadian rhythms. The acute anorectic and weight-reducing effects of NMU and NMS are mediated by NMUR2. This suggests that NMUR2-selective agonists may be useful for the treatment of obesity. | Animals, Calcium Channels, L-Type, Circadian Rhythm, Eating, Energy Metabolism, GTP-Binding Protein alpha Subunits, Gq-G11, Ganglia, Spinal, Homeostasis, Humans, Hypothalamus, Mice, Mitogen-Activated Protein Kinases, Neuropeptides, Obesity, Receptors, Neurotransmitter, Type C Phospholipases, Weight Loss | null |
22,706,106 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | Crossed renal ectopia: can it be a diagnostic problem? | Polak-Jonkisz Dorota, Fornalczyk Konstancja, Musiał Kinga, Zaleska-Dorobisz Urszula, Apoznański Wojciech, Zwolińska Danuta | eng | null | Journal Article | Technetium Tc 99m Dimercaptosuccinic Acid | IM | 22706106, 991491, 10.5604/17322693.991491 | Crossed renal ectopia (C-RE) is a rare congenital anomaly in which both kidneys are located unilaterally. The crossed kidney is situated on the side opposite to its ureteral orifice and usually lies below the normal kidney. The frequency of this malformation is estimated at 0.05% to 0.1%. Most of the patients remain asymptomatic. In other cases C-RE is diagnosed incidentally on routine ultrasonography, due to the presence of unspecific symptoms. The diagnosis of C-RE is possible due to a wide range of imaging techniques: US, IVU, CT, MRI, and TcDMSA scan. Among them IVU, CT, and MRI have the highest degree of confidence. The aim of this retrospective study was to present our own experience with 5 children affected with C-RE, emphasizing the differences in clinical picture and low sensitivity of ultrasound images. In all of them the final diagnosis was established by IVU or MRI. | Child, Child, Preschool, Female, Humans, Incidental Findings, Infant, Kidney, Kidney Diseases, Magnetic Resonance Imaging, Male, Retrospective Studies, Sensitivity and Specificity, Technetium Tc 99m Dimercaptosuccinic Acid, Ultrasonography, Urography | null |
22,706,105 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | The influence of polymorphism of the MUC7 gene on the teeth and dental hygiene of students at a faculty of dentistry in Poland. | Buczkowska-Radlińska Jadwiga, Pol Justyna, Szmidt Monika, Bińczak-Kuleta Agnieszka | eng | null | Journal Article | MUC7 protein, human, Mucins, Salivary Proteins and Peptides | IM | 22706105, 991490, 10.5604/17322693.991490 | The aim of the study was to analyze polymorphism of the MUC7 gene and its correlation with the DMFT value and the Plaque Control Record by O'Leary. | Adult, DMF Index, Dental Caries, Dental Plaque, Dental Plaque Index, Exons, Faculty, Female, Genotype, Humans, Male, Minisatellite Repeats, Mucins, Oral Hygiene, Poland, Polymorphism, Genetic, Prevalence, Salivary Proteins and Peptides, Students, Young Adult | null |
22,706,107 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Carnosine, carnosinase and kidney diseases]. | Kiliś-Pstrusińska Katarzyna | pol | null | Journal Article, Review | Angiotensin-Converting Enzyme Inhibitors, Free Radical Scavengers, Gentamicins, Reactive Oxygen Species, Carnosine, CNDP1 protein, human, Dipeptidases, CNDP2 protein, human, aminoacyl-histidine dipeptidase | IM | 22706107, 991600, 10.5604/17322693.991600 | Carnosine (beta-alanyl-L-histidine) is an endogenously synthesized dipeptide which is present in different human tissues, including the kidney. Carnosine is hydrolyzed by the enzyme carnosinase. There are two carnosinase homologues: serum secreted carnosinase and non-specific cytosolic dipeptidase, encoded by the genes CNDP1 and CNDP2 respectively and located on chromosome 18q22.3. Carnosine functions as a radical oxygen species scavenger and as a natural angiotensin converting enzyme inhibitor. Carnosine inhibits advanced glycation end product formation and reduces the synthesis of matrix proteins such as fibronectin and collagen type VI of podocytes and mesangial cells. In experimental studies it was shown that carnosine reduces the level of proinflammatory and profibrotic cytokines. It is suggested that carnosine is a naturally occurring anti-aging substance in human organisms with a beneficial effect on the cardiovascular system. This paper reports the results of studies concerning carnosine's role in kidney diseases, particularly in ischemia/reperfusion induced acute renal failure, diabetic nephropathy, gentamicin-induced nephrotoxicity and also in blood pressure regulation. The correlations between serum carnosine and serum carnosinase activity and polymorphism in the CNDP1 gene are analyzed. The role of CNDP1 gene polymorphism in the development of diabetic nephropathy and non-diabetic chronic kidney disease is discussed. Carnosine is engaged in different metabolic pathways. It has nephroprotective features. Further studies of carnosine metabolism and its biological properties, particularly those concerning the human organism, are required. | Acute Kidney Injury, Angiotensin-Converting Enzyme Inhibitors, Blood Pressure, Carnosine, Diabetic Nephropathies, Dipeptidases, Free Radical Scavengers, Gentamicins, Humans, Kidney, Kidney Diseases, Polymorphism, Genetic, Reactive Oxygen Species, Reperfusion Injury | null |
22,706,108 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | T cell cytokine synthesis at the single-cell level in BALB/c and C57BL/6 mice infected with ectromelia virus. | Szulc Lidia, Gieryńska Małgorzata, Winnicka Anna, Martyniszyn Lech, Boratyńska-Jasińska Anna, Niemiałtowski Marek | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cytokines, Epitopes, Interleukin-2, Tumor Necrosis Factor-alpha, Interleukin-4, Interferon-gamma | IM | 22706108, 991606, 10.5604/17322693.991606 | The purpose of the study was to evaluate synthesis of IFN-γ, IL-2, TNF-α (Th1/Tc1) and IL-4 (Th2/Tc2) at CD4+ T and CD8+ T cell level in BALB/c and C57BL/6 mice in the course of infection with ectromelia virus Moscow strain (ECTV-MOS). | Animals, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cytokines, Ectromelia virus, Ectromelia, Infectious, Epitopes, Immunity, Cellular, Interferon-gamma, Interleukin-2, Interleukin-4, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, T-Lymphocytes, Tumor Necrosis Factor-alpha | null |
22,706,109 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Mast cells in viral infections]. | Witczak Piotr, Brzezińska-Błaszczyk Ewa | pol | null | Journal Article, Review | Antiviral Agents, Chemokines, Cytokines, Inflammasomes, Toll-Like Receptors, Interferons | IM | 22706109, 991610, 10.5604/17322693.991610 | There are some premises suggesting that mast cells are involved in the mechanisms of anti-virus defense and in viral disease pathomechanisms. Mast cells are particularly numerous at the portals of infections and thus may have immediate and easy contact with the external environment and invading pathogens. These cells express receptors responsible for recognition of virus-derived PAMP molecules, mainly Toll-like receptors (TLR3, TLR7/8 and TLR9), but also RIG-I-like and NOD-like molecules. Furthermore, mast cells generate various mediators, cytokines and chemokines which modulate the intensity of inflammation and regulate the course of innate and adaptive anti-viral immunity. Indirect evidence for the role of mast cells in viral infections is also provided by clinical observations and results of animal studies. Currently, more and more data indicate that mast cells can be infected by some viruses (dengue virus, adenoviruses, hantaviruses, cytomegaloviruses, reoviruses, HIV-1 virus). It is also demonstrated that mast cells can release pre formed mediators as well as synthesize de novo eicosanoids in response to stimulation by viruses. Several data indicate that virus-stimulated mast cells secrete cytokines and chemokines, including interferons as well as chemokines with a key role in NK and Tc lymphocyte influx. Moreover, some information indicates that mast cell stimulation via TLR3, TLR7/8 and TLR9 can affect their adhesion to extracellular matrix proteins and chemotaxis, and influence expression of some membrane molecules. Critical analysis of current data leads to the conclusion that it is not yet possible to make definitive statements about the role of mast cells in innate and acquired defense mechanisms developing in the course of viral infection and/or pathomechanisms of viral diseases. | Antiviral Agents, Chemokines, Cytokines, Dengue Virus, HIV Infections, HIV-1, Humans, Inflammasomes, Interferons, Mast Cells, Toll-Like Receptors, Virus Diseases | null |
22,706,110 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Morganella sp. rods--characteristics, infections, mechanisms of resistance to antibiotics]. | Zalas-Więcek Patrycja, Michalska Anna, Gospodarek Eugenia | pol | null | Journal Article, Review | Hemolysin Proteins, Virulence Factors, Urease | IM | 22706110, 992214, 10.5604/17322693.992214 | The Morganella genus is one member of the tribe Proteae, which also includes the genera Proteus and Providencia. These bacteria are commonly present in the environment. Morganella sp. rods are known to be a causative agent of opportunistic hospital infections, mainly urinary tract, wound and blood infections of severe and high mortality, even in cases of an appropriate antibiotic. These bacteria may produce many virulence factors, for example urease, hemolysins, LPS, adhesins and enzymes hydrolyzing and modifying antibiotics commonly used to treat infections. Understanding the diverse biological properties of these rods may be of importance in the development of effective methods of prevention and control of infections with their participation. | Drug Resistance, Bacterial, Enterobacteriaceae Infections, Hemolysin Proteins, Humans, Morganella, Opportunistic Infections, Urease, Virulence Factors | null |
22,706,111 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Clinical relevance of chemokine receptor CXCR4]. | Gębura Katarzyna, Bogunia-Kubik Katarzyna | pol | null | Journal Article, Review | CXCL12 protein, human, Chemokine CXCL12, Receptors, CXCR4, Granulocyte Colony-Stimulating Factor | IM | 22706111, 997815, 10.5604/17322693.997815 | Stromal cell-derived factor-1 (SDF-1/CXCL12) induces intracellular signaling pathways crucial for mobilization, migration, proliferation and survival of many cell types via CXCR4, a chemokine CXC-motif receptor, member of the G protein-coupled receptor family. Despite playing a key role in such major processes as embryogenesis, cell differentiation and organ regeneration, molecular mechanisms underlying CXCR4 signaling remain elusive, even more so, as CXCR4 seems to activate both G-protein-dependent and G-protein-independent pathways. CXCR4 is expressed on multiple cell types including lymphocytes, hematopoietic stem cells, endothelial and epithelial cells, and cancer cells. In fact, overexpression of this receptor has been detected in many different types of cancer. The SDF-1/CXCR4 axis is also involved in tumor progression, angiogenesis, metastasis, and survival. This pathway is therefore a target for therapeutics that can block the SDF-1/CXCR4 interaction or inhibit downstream intracellular signaling. Clinical mobilization of hematopoietic stem cells (HSC), a nowadays popular method of collecting material for hematopoietic progenitor stem cell transplantation, is also dependent on the SDF-1/CXCR4 axis. Granulocyte colony-stimulating factor (G-CSF), administered to a transplant donor during clinical treatment, violates interactions between CXCR4 and its ligand, which results in degradation of HSC anchorage in bone marrow and the release of these cells into peripheral blood. In this paper we describe the clinical significance of CXCR4 and its ligand, as well as the role of CXCR4 and its gene polymorphisms in disease susceptibility. | Cell Differentiation, Chemokine CXCL12, Disease Progression, Disease Susceptibility, Genetic Predisposition to Disease, Granulocyte Colony-Stimulating Factor, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Humans, Immunologic Deficiency Syndromes, Neoplasms, Neovascularization, Pathologic, Polymorphism, Genetic, Receptors, CXCR4, Signal Transduction, Tissue Engineering | null |
22,706,113 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Oxidation and deamination of nucleobases as an epigenetic tool]. | Guz Jolanta, Jurgowiak Marek, Oliński Ryszard | pol | null | Journal Article, Review | DNA-Binding Proteins, 5-hydroxymethylcytosine, Guanine, 5-Methylcytosine, 8-oxyguanine, Cytosine, DNA | IM | 22706113, 997954, 10.5604/17322693.997954 | Recent discoveries have demonstrated that 5-methylcytosine (5mC) may be hydroxymethylated to 5-hydroxymethylcytosine (5hmC) in mammals and that genomic DNA may contain about 0.02-0.7% of 5hmC. The aforementioned modification is the key intermediate of active DNA demethylation and has been named "the sixth base in DNA". Although active DNA demethylation in mammals is still controversial, the most plausible mechanism/s of active 5mC demethylation include involvement of three families of enzymes; i) Tet, which is involved in hydroxylation of 5mC to form 5hmC, which can be further oxidized to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC); ii) deamination of 5mC (or 5hmC) by AID/APOBEC to form thymine or 5-hydroxymethyluracil (5hmU) mispaired with guanine; iii) the BER pathway induced by involvement of TDG glycosylase to replace the above described base modification (5fC, 5caC, 5hmU) with cytosine to demethylate DNA. A plausible scenario for engagement of TDG glycosylase (or some other G-T glycosylase) is through prior deamination of 5-mC to thymine, which generates a G: T substrate for the enzyme. Here cytidine deaminase of the AID/APOBEC family was implicated in the deamination step. It is possible that TDG may act in concert with these deaminases. It seems that mutations are not the only effect of oxidatively modified DNA bases. These, as yet, understudied aspects of the damage suggest a potential for 8-oxoguanine (8-oxoGua) to affect gene expression via chromatin relaxation. It is possible that 8-oxoGua presence in specific DNA sequences may be widely used for transcription regulation, which suggests the epigenetic nature of 8-oxoGua presence in DNA. | 5-Methylcytosine, Animals, Base Composition, Chromatin Assembly and Disassembly, Cytosine, DNA, DNA Methylation, DNA-Binding Proteins, Deamination, Epigenomics, Gene Expression Regulation, Guanine, Mutation, Neoplasms, Oxidation-Reduction, Sequence Analysis, DNA, Transcription, Genetic | null |
22,706,112 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Leptin as a mediator between obesity and cardiac dysfunction]. | Karbowska Joanna, Kochan Zdzisław | pol | null | Journal Article, Review | Leptin | IM | 22706112, 997817, 10.5604/17322693.997817 | Obesity is now recognised as one of the most important risk factors for heart disease. Obese individuals have high circulating levels of leptin, a hormone secreted by adipose tissue and involved in energy homeostasis. Growing evidence suggests that leptin may contribute to the development of cardiac dysfunction. In a large prospective study leptin has been shown to be an independent risk factor for coronary heart disease. An independent positive association has also been found between plasma leptin levels and heart rate in hypertensive patients and heart transplant recipients. In animal studies chronic leptin infusion increased heart rate and blood pressure. It has also been demonstrated that circulating leptin levels are elevated in patients with heart failure. The level of plasma leptin was associated with increased myocardial wall thickness and correlated with left ventricular mass, suggesting a role for this hormone in mediating left ventricular hypertrophy in humans. Moreover, leptin directly induced hypertrophy and hyperplasia in human and rodent cardiomyocytes, accompanied by cardiac extracellular matrix remodelling. Leptin may also influence energy substrate utilisation in cardiac tissue. These findings suggest that leptin acting directly or through the sympathetic nervous system may have adverse effects on cardiac structure and function, and that chronic hyperleptinaemia may greatly increase the risk of cardiac disorders. Additional studies are needed to define the role of leptin in cardiac physiology and pathophysiology, nevertheless the reduction in plasma leptin levels with caloric restriction and weight loss may prevent cardiac dysfunction in obese patients. | Adipose Tissue, Cardiovascular Diseases, Coronary Artery Disease, Heart Rate, Humans, Hypertension, Hypertrophy, Left Ventricular, Leptin, Myocardium, Myocytes, Cardiac, Obesity, Ventricular Remodeling, Weight Loss | null |
22,706,114 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | Potential therapeutic interventions via EP2/EP4 prostaglandin receptors. | Zimecki Michał | eng | null | Journal Article, Review | Prostaglandins, Receptors, Prostaglandin E, EP2 Subtype, Receptors, Prostaglandin E, EP4 Subtype, Dinoprostone | IM | 22706114, 998859, 10.5604/17322693.998859 | Prevention and treatment of pathological inflammatory processes requires application of various classes of immune suppressors, such as calcineurin inhibitors, steroids and non-steroid inhibitors of prostaglandin synthesis. However, each type of these immune suppressors causes less or more serious adverse side-effects. Exploration of the role played by prostanoids in the immune response and identification of functionally distinct prostaglandin E receptors (EP1-EP4) opened new perspectives in therapy of inflammation, autoimmunity and prevention of graft rejection. The EP4 receptor appeared to be an attractive target to affect manifestations of various pathological states by application of either agonists or antagonists of the receptor. This article presents a short overview of experimental approaches aimed at manipulation of signaling via EP2 and EP4 receptors that could have therapeutic utility. | Animals, Arthritis, Autoimmunity, Dinoprostone, Gastroenteritis, Graft Rejection, Humans, Hypersensitivity, Inflammation, Prostaglandins, Receptors, Prostaglandin E, EP2 Subtype, Receptors, Prostaglandin E, EP4 Subtype, Reperfusion Injury | null |
22,706,115 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [The role of ceramides in selected brain pathologies: ischemia/hypoxia, Alzheimer disease]. | Car Halina, Zendzian-Piotrowska Małgorzata, Fiedorowicz Anna, Prokopiuk Sławomir, Sadowska Anna, Kurek Krzysztof | pol | null | Journal Article, Review | Ceramides, Sphingolipids, Sphingomyelins, Caspase 3 | IM | 22706115, 999024, 10.5604/17322693.999024 | Ceramides, members of the sphingolipids, are produced in the central nervous system by de novo synthesis, sphingomyelin hydrolysis or the so-called salvage pathway. They are engaged in formation of lipid rafts that are essential in regulation and transduction of signals coming to the cell from the environment. Ceramides represent the major transmitters of the sphingomyelin pathway of signal transduction. They regulate proliferation, differentiation, programmed cell death and senescence. Ceramide overexpression, mainly as a result of sphingomyelin hydrolysis, is a component of brain damage caused by ischemia and early reperfusion. Their high concentrations induce mitochondria-dependent neuronal apoptosis, exacerbate the synthesis of reactive oxygen species, decrease ATP level, inhibit electron transport and release cytochrome c, and activate caspase-3. Reduced ceramide accumulation in the brain, dependent mainly on ceramide synthesized de novo, may exert an anti-apoptotic effect after pre-conditioning. The increase of ceramide content in the brain was observed in Alzheimer disease and its animal models. Enhanced ceramide concentration in this pathology is an effect of their synthesis de novo or sphingomyelin metabolism augmentation. The ceramide pathway can directly stimulate biochemical changes in the brain noted at the onset of disease: tau overphosphorylation and β-amyloid peptide accumulation. The higher concentration of ceramides in blood in the pre-clinical phase of the illness may mark early brain changes. | Alzheimer Disease, Animals, Apoptosis, Brain, Brain Ischemia, Caspase 3, Cell Differentiation, Ceramides, Humans, Mitochondria, Signal Transduction, Sphingolipids, Sphingomyelins | null |
22,706,116 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [p75NTR receptor--role in cell growth and apoptosis]. | Urbaniak Anna | pol | null | Journal Article, Review | Nerve Growth Factors, Protein Isoforms, Receptor, Nerve Growth Factor | IM | 22706116, 999026, 10.5604/17322693.999026 | The neurotrophins play an important role in the development of the nervous system. These trophic factors affect the cells through the neurotrophin receptors Trk and p75NTR. Trk (tyrosine kinase receptor) mediated signaling promotes survival and growth, while p75NTR-mediated signaling promotes cell death. The structure of p75NTR and its role in the regulation of survival, growth and induction of apoptosis are discussed. p75NTR can interact with the aggregated form of Aβ peptides and by influencing protein tau hyperphosphorylation plays an important role in etiopathogenesis of Alzheimer's disease. | Amino Acid Sequence, Animals, Apoptosis, Cell Cycle, Cell Proliferation, Heredodegenerative Disorders, Nervous System, Humans, Nerve Growth Factors, Protein Isoforms, Receptor, Nerve Growth Factor, Signal Transduction | null |
22,706,118 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [In vitro renaturation of proteins from inclusion bodies]. | Porowińska Dorota, Marszałek Ewelina, Wardęcka Paulina, Komoszyński Michał | pol | null | Journal Article, Review | Molecular Chaperones, Recombinant Proteins | IM | 22706118, 999918, 10.5604/17322693.999918 | Recombinant proteins and enzymes are commonly used in many areas of our life, such as diagnostics, industry and medicine, due to heterologous synthesis in prokaryotic expression systems. However, a high expression level of foreign protein in bacteria cells results in formation of inactive and insoluble aggregates--inclusion bodies. Reactivation of aggregated proteins is a complex and time-consuming process. Every protein requires experimental optimization of the process conditions. The choice of the refolding method depends on the type of recombinant protein and its physical, chemical and biological properties. Recovery of the activity of proteins accumulated in inclusion bodies can be divided into 4 steps: 1) inclusion bodies isolation, 2) solubilization of aggregates, 3) renaturation, 4) purification of catalytically active molecules. Efficiency of the refolding process depends on many physical factors and chemical and biological agents. The above parameters determine the time of the folding and prevent protein aggregation. They also assist the folding and have an influence on the solubility and stability of native molecules. To date, dilution, dialysis and chromatography are the most often used methods for protein refolding. | Chromatography, Dialysis, Escherichia coli, Inclusion Bodies, Molecular Chaperones, Protein Renaturation, Recombinant Proteins, Solubility | null |
22,706,117 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [PARP inhibitors--theoretical basis and clinical application]. | Dębska Sylwia, Kubicka Joanna, Czyżykowski Rafał, Habib Maja, Potemski Piotr | pol | null | Journal Article, Review | Antineoplastic Agents, Enzyme Inhibitors, Poly(ADP-ribose) Polymerase Inhibitors, Poly(ADP-ribose) Polymerases | IM | 22706117, 999033, 10.5604/17322693.999033 | Poly-ADP-ribose polymerases (PARP) are involved in a number of processes that are vital for every living cell. Once activated by the presence of DNA damage they trigger poly-ADP-ribosylation of various proteins which are crucial for DNA repair, preserving of genom integrity, regulation of transcription, proliferation and apoptosis. PARP1, which is the best known enzyme of PARP protein family, plays a role in single-strand breaks (SSB) repair. Decrease of its activity results in accumulation of single strand DNA breaks (SSB) which leads as a consequence to double-strand breaks (DSBs). This disorder is particularly harmful to cells with deficiency of BRCA1/2 protein which is involved in repair of DNA double-strand breaks. This phenomenon is an example of "synthetic lethality" concept and contributes to research on application of PARP inhibitors in treatment of cancers associated with BRCA1/2 protein defect (breast or ovarian cancer). Noticed synergism between PARP inhibitors and genotoxic chemotherapy or radiotherapy determined another direction of research on application of these medicaments. After promising results of phase I and II trials with most commonly investigated PARP inhibitors--iniparib and olaparib--which recruited patients with triple negative breast cancer and ovarian cancer, further studies started. This paper presents theoretical basis of PARP inhibitors action as well as critical review of most important clinical trials of these medicaments. | Antineoplastic Agents, Breast Neoplasms, DNA Breaks, Double-Stranded, DNA Breaks, Single-Stranded, DNA Damage, DNA Repair, Enzyme Inhibitors, Female, Genes, BRCA1, Genes, BRCA2, Humans, Mutation, Ovarian Neoplasms, Poly(ADP-ribose) Polymerase Inhibitors, Poly(ADP-ribose) Polymerases | null |
22,706,119 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [DNA microarray-based gene expression profiling in diagnosis, assessing prognosis and predicting response to therapy in colorectal cancer]. | Kwiatkowski Przemysław, Wierzbicki Piotr, Kmieć Andrzej, Godlewski Janusz | pol | null | Journal Article, Review | Genetic Markers, DNA | IM | 22706119, 999919, 10.5604/17322693.999919 | Colorectal cancer is the most common cancer of the gastrointestinal tract. It is considered as a biological model of a certain type of cancerogenesis process in which progression from an early to late stage adenoma and cancer is accompanied by distinct genetic alterations. Clinical and pathological parameters commonly used in clinical practice are often insufficient to determine groups of patients suitable for personalized treatment. Moreover, reliable molecular markers with high prognostic value have not yet been determined. Molecular studies using DNA-based microarrays have identified numerous genes involved in cell proliferation and differentiation during the process of cancerogenesis. Assessment of the genetic profile of colorectal cancer using the microarray technique might be a useful tool in determining the groups of patients with different clinical outcomes who would benefit from additional personalized treatment. The main objective of this study was to present the current state of knowledge on the practical application of gene profiling techniques using microarrays for determining diagnosis, prognosis and response to treatment in colorectal cancer. | Adenoma, Colorectal Neoplasms, DNA, Disease Progression, Gene Expression Profiling, Genetic Markers, Humans, Medical Oncology, Mutation, Oligonucleotide Array Sequence Analysis, Prognosis, Transcriptome, Treatment Outcome | null |
22,706,120 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | Results of antiviral treatment of patients with chronic hepatitis C: experience of Poznan centre. | Bura Maciej, Kowala-Piaskowska Arleta, Adamek Agnieszka, Bura Aleksandra, Czajka Arkadiusz, Hryckiewicz Katarzyna, Bereszyńska Iwona, Mozer-Lisewska Iwona | eng | null | Journal Article | Antiviral Agents, Interferon-alpha, Recombinant Proteins, Polyethylene Glycols, Ribavirin, Alanine Transaminase, peginterferon alfa-2a | IM | 22706120, 1000332, 10.5604/17322693.1000332 | Hepatitis C virus (HCV) infection in Poland affects approximately 750 thousand persons. The prevention of cirrhosis and hepatocellular carcinoma, of which approximately 20% of patients with chronic hepatitis C virus are at risk, aims at eradication of the virus by applying antiviral treatment with pegylated interferon alpha with ribavirin. | Adolescent, Adult, Age Factors, Aged, Alanine Transaminase, Antiviral Agents, Biopsy, Drug Therapy, Combination, Female, Hepatitis C, Chronic, Humans, Interferon-alpha, Liver, Male, Middle Aged, Polyethylene Glycols, Recombinant Proteins, Ribavirin, Treatment Outcome, Viremia, Young Adult | null |
22,706,121 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | Variable fragments of heavy chain antibodies (VHHs): a new magic bullet molecule of medicine? | Smolarek Dorota, Bertrand Olivier, Czerwinski Marcin | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Antineoplastic Agents, Immunoglobulin Fragments, Immunoglobulin Heavy Chains, Single-Domain Antibodies | IM | 22706121, 1000334, 10.5604/17322693.1000334 | Serum of animals belonging to the Camelidae family (camels and llamas) contains fully active antibodies that are naturally devoid of light chains. Variable domains derived from heavy chain antibodies (hcAb) called VHHs or nanobodies™ can bind antigens as effectively as full-length antibodies and are easy to clone and express. Because of their potential, VHHs are being intensively studied as potential therapeutic, diagnostic and imaging tools. The paper reviews the molecular background of heavy chain antibodies and describes methods of obtaining recombinant fragments of heavy chain antibodies as well as their therapeutic, diagnostic and other applications. | Animals, Antineoplastic Agents, Autoimmune Diseases, Camelids, New World, Chromatography, Affinity, Communicable Diseases, Fishes, Hematologic Diseases, Humans, Immunoglobulin Fragments, Immunoglobulin Heavy Chains, Single-Domain Antibodies, Species Specificity | null |
22,706,122 | 2013-05-02 | 2023-12-13 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Mutations of noncollagen genes in osteogenesis imperfecta--implications of the gene products in collagen biosynthesis and pathogenesis of disease]. | Galicka Anna | pol | null | Journal Article, Review | CRTAP protein, human, Collagen Type I, Extracellular Matrix Proteins, Eye Proteins, Molecular Chaperones, Nerve Growth Factors, Proteins, Serpins, Sp7 Transcription Factor, SP7 protein, human, Transcription Factors, pigment epithelium-derived factor, cyclophilin B, Procollagen-Proline Dioxygenase, BMP1 protein, human, Bone Morphogenetic Protein 1, Cyclophilins | IM | 22706122, 1000336, 10.5604/17322693.1000336 | Recent investigations revealed that the "brittle bone" phenotype in osteogenesis imperfecta (OI) is caused not only by dominant mutations in collagen type I genes, but also by recessively inherited mutations in genes responsible for the post-translational processing of type I procollagen as well as for bone formation. The phenotype of patients with mutations in noncollagen genes overlaps with very severe type III and lethal type II OI caused by mutations in collagen genes. Mutations in genes that encode proteins involved in collagen prolyl 3-hydroxylation (P3H1/CRTAP/CyPB) eliminated Pro986 hydroxylation and caused an increase in modification of collagen helix by prolyl 4-hydroxylase and lysyl hydroxylase. However, the importance of these disturbances in the disease pathomechanism is not known. Loss of complex proteins' function as collagen chaperones may dominate the disease mechanism. The latest findings added to the spectrum of OI-causing and collagen-influencing factors other chaperones (HSP47 and FKBP65) and protein BMP-1, which emphasizes the complexity of collagen folding and secretion as well as their importance in bone formation. Furthermore, mutations in genes encoding transcription factor SP7/Osterix and pigment epithelium-derived factor (PEDF) constitute a novel mechanism for OI, which is independent of changes in biosynthesis and processing of collagen. | Bone Morphogenetic Protein 1, Collagen Type I, Cyclophilins, Extracellular Matrix Proteins, Eye Proteins, Genes, Recessive, Humans, Molecular Chaperones, Mutation, Nerve Growth Factors, Open Reading Frames, Osteogenesis Imperfecta, Procollagen-Proline Dioxygenase, Protein Processing, Post-Translational, Proteins, Serpins, Sp7 Transcription Factor, Transcription Factors | null |
22,706,123 | 2013-05-02 | 2019-11-12 | 1732-2693 | Postepy higieny i medycyny doswiadczalnej (Online) | [Poly(ADP-ribose) polymerase (PARP) inhibitors in BRCA1/2 cancer therapy]. | Kluzek Katarzyna, Białkowska Aneta, Koczorowska Aleksandra, Zdzienicka Małgorzata Z | pol | null | Journal Article, Review | Antineoplastic Agents, Enzyme Inhibitors, Poly(ADP-ribose) Polymerase Inhibitors | IM | 22706123, 1000548, 10.5604/17322693.1000548 | A majority of currently used anticancer drugs belong to a group of chemical agents that damage DNA. The efficiency of the treatment is limited by effective DNA repair systems functioning in cancer cells. Many chemotherapeutic compounds cause strong systemic toxicity. Therefore, there is still a need for new anticancer agents which are less toxic for nontransformed cells and selectively kill cancer cells. One of the most promising molecular targets in cancer therapy is poly(ADP-ribose) polymerases (PARP). PARP play an essential role in repairing DNA strand breaks. Small molecule inhibitors of these enzymes have been developed and have proved to be extremely toxic for cancer cells that lack the functional BRCA1 and BRCA2 proteins that are involved in homologous recombination, a complex repair mechanism of DNA double strand breaks. Mutations in BRCA1/2 genes are associated with genetically inherited breast and ovarian cancers. Therefore PARP inhibitors may prove to be very effective and selective in the treatment of these cancer types. This review is focused on the function of BRCA1/2 proteins and poly(ADP-ribose) polymerases in DNA repair systems, especially in the homologous recombination process. A short history of the studies that led to synthesis of high specificity small molecule PARP inhibitors is also presented, as well as the results of clinical trials concerning the most effective PARP inhibitors in view of their potential application in oncological treatment, particularly breast cancers. | Antineoplastic Agents, Breast Neoplasms, DNA Breaks, Double-Stranded, DNA Repair, Enzyme Inhibitors, Female, Genes, BRCA1, Genes, BRCA2, Humans, Ovarian Neoplasms, Poly(ADP-ribose) Polymerase Inhibitors | null |
22,706,124 | 2012-08-28 | 2018-12-01 | 1530-0358 | Diseases of the colon and rectum | Mathews redux. | Cosman Bard C | eng | null | Editorial, Comment | null | IM | 22706124, 10.1097/DCR.0b013e3182569b3c, 00003453-201207000-00001 | null | Digestive System Surgical Procedures, Female, Humans, Male, Rectal Fistula | null |
22,706,126 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Total fistulectomy with simple closure of the internal opening in the management of complex cryptoglandular fistulas: long-term results and functional outcome. | Tobisch Alexander, Stelzner Sigmar, Hellmich Gunter, Jackisch Thomas, Witzigmann Helmut | eng | null | Journal Article | null | IM | 22706126, 10.1097/DCR.0b013e3182569b29, 00003453-201207000-00003 | Total fistulectomy with simple closure of the internal opening has been used for the management of complex anal fistulas. This approach involves complete removal of the fistula tract and closure of the internal opening with sutures. | Adult, Age Factors, Aged, Aged, 80 and over, Digestive System Surgical Procedures, Fecal Incontinence, Female, Follow-Up Studies, Humans, Male, Middle Aged, Postoperative Complications, Rectal Fistula, Retrospective Studies, Suture Techniques, Treatment Outcome, Young Adult | null |
22,706,125 | 2012-08-28 | 2013-02-20 | 1530-0358 | Diseases of the colon and rectum | Practice parameters for anal squamous neoplasms. | Steele Scott R, Varma Madhulika G, Melton Genevieve B, Ross Howard M, Rafferty Janice F, Buie W Donald | eng | null | Journal Article, Practice Guideline | null | IM | 22706125, 10.1097/DCR.0b013e318255815e, 00003453-201207000-00002 | null | Anus Neoplasms, Carcinoma, Squamous Cell, Evidence-Based Medicine, Humans, Lymphatic Metastasis, Neoplasm Staging | null |
22,706,127 | 2012-08-28 | 2022-03-31 | 1530-0358 | Diseases of the colon and rectum | Timing of restorative proctocolectomy in patients with medically refractory ulcerative colitis: the patient's point of view. | Neumann Philipp-Alexander, Mennigen Rudolf B, Senninger Norbert, Bruewer Matthias, Rijcken Emile | eng | null | Journal Article | null | IM | 22706127, 10.1097/DCR.0b013e318251e004, 00003453-201207000-00004 | Development of biologic agents has led to new therapeutic options for patients with refractory ulcerative colitis, and intensive medical therapy allows delay of restorative colectomy. However, the overall rate of colectomies has not changed. The decision as to timing of the operation is difficult. | Adolescent, Adult, Anastomosis, Surgical, Colitis, Ulcerative, Female, Follow-Up Studies, Humans, Male, Patient Satisfaction, Proctocolectomy, Restorative, Retrospective Studies, Surveys and Questionnaires, Time Factors, Young Adult | null |
22,706,128 | 2012-08-28 | 2022-04-08 | 1530-0358 | Diseases of the colon and rectum | Autologous expanded adipose-derived stem cells for the treatment of complex cryptoglandular perianal fistulas: a phase III randomized clinical trial (FATT 1: fistula Advanced Therapy Trial 1) and long-term evaluation. | Herreros M D, Garcia-Arranz M, Guadalajara H, De-La-Quintana P, Garcia-Olmo D | eng | null | Clinical Trial, Phase III, Journal Article, Multicenter Study, Randomized Controlled Trial | Fibrin Tissue Adhesive, Tissue Adhesives | IM | 22706128, 10.1097/DCR.0b013e318255364a, 00003453-201207000-00005 | Autologous adipose-derived stem cells may represent a novel approach for the management of complex fistula-in-ano. After successful phase I and II clinical trials, a phase III trial was performed to investigate the safety and efficacy. | Adipocytes, Adult, Combined Modality Therapy, Female, Fibrin Tissue Adhesive, Follow-Up Studies, Humans, Male, Mesenchymal Stem Cell Transplantation, Middle Aged, Rectal Fistula, Single-Blind Method, Spain, Tissue Adhesives, Transplantation, Autologous, Treatment Outcome | null |
22,706,129 | 2012-08-28 | 2022-03-30 | 1530-0358 | Diseases of the colon and rectum | The spectrum of perianal Crohn's disease in a population-based cohort. | Eglinton Tim W, Barclay Murray L, Gearry Richard B, Frizelle Frank A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706129, 10.1097/DCR.0b013e31825228b0, 00003453-201207000-00006 | Perianal Crohn's disease represents a phenotype distinct from luminal Crohn's disease and may follow a different course. To date, the only detailed classifications of perianal Crohn's disease arise from referral center cohorts that do not reflect the spectrum of disease in the population as a whole. | Adolescent, Adult, Aged, Aged, 80 and over, Anus Diseases, Child, Child, Preschool, Cohort Studies, Crohn Disease, Disease Progression, Female, Follow-Up Studies, Humans, Male, Middle Aged, New Zealand, Retrospective Studies, Time Factors, Young Adult | null |
22,706,130 | 2012-08-28 | 2022-03-21 | 1530-0358 | Diseases of the colon and rectum | Ligation of intersphincteric fistula tract: early results of a pilot study. | Abcarian Ariane M, Estrada Joaquin J, Park John, Corning Cybil, Chaudhry Vivek, Cintron Jose, Prasad Leela, Abcarian Herand | eng | null | Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't | null | IM | 22706130, 10.1097/DCR.0b013e318255ae8a, 00003453-201207000-00007 | Transsphincteric fistulotomy is associated with a variable degree of fecal incontinence that is directly related to the thickness of the sphincter mechanism overlying the fistula. Staged fistulotomy with seton or the use of cutting seton designed to reduce the proportionate incontinence rates have failed to do so. This has resulted in attempts to find novel sphincter-sparing techniques in the past 2 decades including draining seton, fibrin sealant, anal fistula plug, dermal advancement, and endorectal advancement flaps. These operations have a variable success rates of 30% to 80% reported in the literature. | Adult, Aged, Digestive System Surgical Procedures, Fecal Incontinence, Follow-Up Studies, Humans, Ligation, Middle Aged, Pilot Projects, Postoperative Complications, Rectal Fistula, Suture Techniques, Treatment Outcome, Young Adult | null |
22,706,132 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Poor 1-year survival in elderly patients undergoing nonelective colorectal resection. | Mamidanna Ravikrishna, Eid-Arimoku Lola, Almoudaris Alex M, Burns Elaine M, Bottle Alex, Aylin Paul, Hanna George B, Faiz Omar | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706132, 10.1097/DCR.0b013e3182585a35, 00003453-201207000-00009 | Colorectal resection in elderly patients is associated with significant morbidity and mortality, especially in an emergency setting. | Adult, Aged, Aged, 80 and over, Colectomy, Fecal Incontinence, Female, Follow-Up Studies, Germany, Hospitals, Community, Humans, Male, Middle Aged, Postoperative Complications, Rectal Fistula, Reoperation, Retrospective Studies, Survival Rate, Treatment Outcome, Young Adult | null |
22,706,131 | 2012-08-28 | 2022-03-17 | 1530-0358 | Diseases of the colon and rectum | The impact of preoperative stoma site marking on the incidence of complications, quality of life, and patient's independence. | Person Benjamin, Ifargan Ruth, Lachter Jesse, Duek Simon D, Kluger Yoram, Assalia Ahmad | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706131, 10.1097/DCR.0b013e31825763f0, 00003453-201207000-00008 | Preoperative stoma site marking and counseling aim to improve patients' rehabilitation and adaptation to a new medical condition. Objective studies are needed to provide evidence of the impact of care by stoma therapists. Key quality indicators include patients' quality of life, independence, and complication rates as affected by the variable modes of stoma site marking and planning. | Adolescent, Adult, Aged, Aged, 80 and over, Colostomy, Female, Humans, Ileostomy, Incidence, Male, Middle Aged, Postoperative Complications, Preoperative Care, Quality of Life, Self Care, Surgical Stomas, Surveys and Questionnaires, Urologic Surgical Procedures, Young Adult | null |
22,706,134 | 2012-08-28 | 2022-03-31 | 1530-0358 | Diseases of the colon and rectum | Mesenteric panniculitis: a paraneoplastic phenomenon? | Wilkes Anna, Griffin Nick, Dixon Liane, Dobbs Bruce, Frizelle Frank A | eng | null | Journal Article | null | IM | 22706134, 10.1097/DCR.0b013e318252e286, 00003453-201207000-00011 | Mesenteric panniculitis is an inflammatory condition of mesenteric adipose tissue with characteristic features on abdominal CT imaging. Although its cause is unknown, it has been associated with malignancy. | Adult, Aged, Aged, 80 and over, Colorectal Neoplasms, Female, Humans, Lymphoma, Male, Middle Aged, New Zealand, Panniculitis, Peritoneal, Paraneoplastic Syndromes, Prevalence, Retrospective Studies, Tomography, X-Ray Computed, Urogenital Neoplasms, Young Adult | null |
22,706,133 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Sacral nerve stimulation for fecal incontinence related to external sphincter atrophy. | Santoro Giulio A, Infantino Aldo, Cancian Luca, Battistella Giuseppe, Di Falco Giuseppe | eng | null | Journal Article | null | IM | 22706133, 10.1097/DCR.0b013e3182538f14, 00003453-201207000-00010 | Atrophy of the external anal sphincter, a pathologic muscle volume anomaly associated with fecal incontinence, has been shown to be a negative predictor of the outcome of surgery for defects of the external anal sphincter. It is unclear whether external anal sphincter atrophy also affects the outcome of sacral nerve stimulation for fecal incontinence. | Adult, Aged, Aged, 80 and over, Anal Canal, Atrophy, Fecal Incontinence, Female, Follow-Up Studies, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Prospective Studies, Quality of Life, Sacrum, Severity of Illness Index, Transcutaneous Electric Nerve Stimulation, Treatment Outcome | null |
22,706,135 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Familial adenomatous polyposis-related desmoids presenting with air-fluid level: a clinical review and management algorithm. | Bhandari Santosh, Ranchod Pravin, Sinha Ashish, Gupta Arun, Clark Susan K, Phillips Robin K S | eng | null | Journal Article | Anti-Bacterial Agents | IM | 22706135, 10.1097/DCR.0b013e318257fa93, 00003453-201207000-00012 | Familial adenomatous polyposis-related desmoid tumors can present with a liquefied center containing gas, accompanied by abdominal pain and sepsis. To date the optimal management of such patients has not been documented. | Adenomatous Polyposis Coli, Adult, Algorithms, Anti-Bacterial Agents, Cohort Studies, Combined Modality Therapy, Digestive System Surgical Procedures, Drainage, Female, Fibromatosis, Abdominal, Humans, Male, Peritonitis, Registries, Retrospective Studies, Sepsis, Tomography, X-Ray Computed, Young Adult | null |
22,706,136 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Single-scar laparoscopic colectomy with intracorporeal attachable and detachable instruments. | Uematsu Dai, Magishi Akiko, Sano Takayuki, Niitsu Hirokazu | eng | null | Journal Article | null | IM | 22706136, 10.1097/DCR.0b013e318252cc68, 00003453-201207000-00013 | In single-access laparoscopic colectomy, the number of instruments that can be inserted through the single-access site is limited by instrument collision. To compensate, triangulation is necessary, but the operative field becomes inadequate. To overcome this problem, intracorporeal attachable and detachable instruments can broaden the field of visceral tissue by retracting from at least 2 points. | Aged, Aged, 80 and over, Blood Loss, Surgical, Colectomy, Colonic Neoplasms, Feasibility Studies, Female, Humans, Laparoscopy, Length of Stay, Male, Middle Aged, Postoperative Complications, Prospective Studies, Surgical Instruments, Treatment Outcome | null |
22,706,137 | 2012-08-28 | 2019-12-10 | 1530-0358 | Diseases of the colon and rectum | Meta-analysis of laparoscopic versus open colorectal surgery within fast-track perioperative care. | Li Ming-zhe, Xiao Long-bin, Wu Wen-hui, Yang Shi-bin, Li Shou-zhi | eng | null | Comparative Study, Journal Article, Meta-Analysis | null | IM | 22706137, 10.1097/DCR.0b013e31824bd31e, 00003453-201207000-00014 | Both laparoscopic surgery and fast-track perioperative care have demonstrated advantages in patients undergoing elective colorectal resections. It is unclear whether there is an additive effect by combining these 2 procedures. | Colorectal Surgery, Humans, Laparoscopy, Length of Stay, Morbidity, Mortality, Outcome Assessment, Health Care, Patient Readmission, Perioperative Care, Randomized Controlled Trials as Topic | null |
22,706,139 | 2012-08-28 | 2018-12-01 | 1530-0358 | Diseases of the colon and rectum | Different surgeons find same solution for minimally invasive stoma construction. | Atallah Sam | eng | null | Letter, Comment | null | IM | 22706139, 10.1097/DCR.0b013e3182542383, 00003453-201207000-00017 | null | Crohn Disease, Female, Humans, Ileostomy, Laparoscopy, Male, Rectal Fistula | null |
22,706,140 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Self-assessment quiz: answers, critiques, and references. | Margolin David A | eng | null | Journal Article | null | IM | 22706140, 10.1097/DCR.0b013e318254a17f, 00003453-201207000-00018 | null | Accreditation, Colorectal Surgery, Education, Continuing, Humans, Self-Evaluation Programs | null |
22,706,138 | 2012-08-28 | 2012-06-18 | 1530-0358 | Diseases of the colon and rectum | Delivering omental coverage to the pelvis following proctectomy in the prone position: a technical tip. | Carr William R, Wakefield Simon E | eng | null | Letter | null | IM | 22706138, 10.1097/DCR.0b013e318256f2bd, 00003453-201207000-00016 | null | Humans, Omentum, Pelvis, Postoperative Complications, Prone Position, Rectum | null |
22,706,146 | 2012-11-30 | 2017-11-16 | 1879-1026 | The Science of the total environment | Factors affecting vertical distribution of Fukushima accident-derived radiocesium in soil under different land-use conditions. | Koarashi Jun, Atarashi-Andoh Mariko, Matsunaga Takeshi, Sato Tsutomu, Nagao Seiya, Nagai Haruyasu | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cesium Radioisotopes, Soil, Soil Pollutants, Radioactive | IM | 22706146, S0048-9697(12)00723-1, 10.1016/j.scitotenv.2012.05.041 | The Fukushima Dai-ichi nuclear power plant accident in Japan, triggered by a big earthquake and the resulting tsunami on 11 March 2011, caused a substantial release of radiocesium ((137)Cs and (134)Cs) and a subsequent contamination of soils in a range of terrestrial ecosystems. Identifying factors and processes affecting radiocesium retention in these soils is essential to predict how the deposited radiocesium will migrate through the soil profile and to other biological components. We investigated vertical distributions of radiocesium and physicochemical properties in soils (to 20 cm depth) at 15 locations under different land-use types (croplands, grasslands, and forests) within a 2 km × 2 km mesh area in Fukushima city. The total (137)Cs inventory deposited onto and into soil was similar (58.4±9.6 kBq m(-2)) between the three different land-use types. However, aboveground litter layer at the forest sites and herbaceous vegetation at the non-forested sites contributed differently to the total (137)Cs inventory. At the forest sites, 50-91% of the total inventory was observed in the litter layer. The aboveground vegetation contribution was in contrast smaller (<35%) at the other sites. Another remarkable difference was found in vertical distribution of (137)Cs in mineral soil layers; (137)Cs penetrated deeper in the forest soil profiles than in the non-forested soil profiles. We quantified (137)Cs retention at surface soil layers, and showed that higher (137)Cs retention can be explained in part by larger amounts of silt- and clay-sized particles in the layers. More importantly, the (137)Cs retention highly and negatively correlated with soil organic carbon content divided by clay content across all land-use types. The results suggest that organic matter inhibits strong adsorption of (137)Cs on clay minerals in surface soil layers, and as a result affects the vertical distribution and thus the mobility of (137)Cs in soil, particularly in the forest ecosystems. | Agriculture, Cesium Radioisotopes, Ecosystem, Japan, Plants, Radioactive Hazard Release, Soil, Soil Pollutants, Radioactive, Trees | null |
22,706,145 | 2013-01-08 | 2012-08-20 | 1096-0295 | Regulatory toxicology and pharmacology : RTP | Safety evaluation of a lipase enzyme (BD29241 Palmitase) preparation, expressed in Pseudomonas fluorescens, intended for removing palmitic acid from triacylglycerol. | Halich Roxanna, Kline Katie, Shanahan Diane, Ciofalo Vince | eng | null | Journal Article | Mutagens, Plant Oils, Triglycerides, Carboxylic Ester Hydrolases, long-chain carboxylesterase | IM | 22706145, S0273-2300(12)00116-X, 10.1016/j.yrtph.2012.06.006 | The lipase enzyme, BD29241 Palmitase, can be used as a processing aid for removing palmitic acid from triacylglycerol in the production of refined oil. This enzyme was produced from a Pseudomonas fluorescens (P. fluorescens) production strain and was tested in acute, inhalation, and subchronic toxicity studies. In addition, this enzyme was also tested for its potential to induce genotoxicity. Dosages of the test article preparation ranged from 5000μg/plate for in vitro toxicity studies to 2000mg/kg/day for in vivo toxicity studies. The highest oral dose tested in vivo (NOAEL of 2000mg/kg/day) resulted in a safety margin of 2.442×10(3) based on a conservative estimate of the total human consumption of BD29241 Palmitase of 0.819mg/kg/day. There was no toxicity reported for any of these studies including additional safety studies. A review of the literature indicates that P. fluorescens fulfills recognized safety criteria pertinent to microbial production strains used in the manufacture of food enzyme preparations. The results of the toxicity studies presented herein attest to the safety of BD29241 Palmitase for its above-stated intended use. | Animals, Carboxylic Ester Hydrolases, Chromosome Aberrations, Dermatitis, Allergic Contact, Dose-Response Relationship, Drug, Eye Diseases, Guinea Pigs, Humans, Inhalation Exposure, Lethal Dose 50, Lymphocytes, Mice, Micronuclei, Chromosome-Defective, Mutagens, No-Observed-Adverse-Effect Level, Plant Oils, Pseudomonas fluorescens, Rabbits, Rats, Rats, Sprague-Dawley, Risk Assessment, Safety, Salmonella typhimurium, Toxicity Tests, Triglycerides | null |
22,706,147 | 2012-11-30 | 2013-11-21 | 1879-1026 | The Science of the total environment | Hydrogeochemical contrast between brown and grey sand aquifers in shallow depth of Bengal Basin: consequences for sustainable drinking water supply. | Biswas Ashis, Nath Bibhash, Bhattacharya Prosun, Halder Dipti, Kundu Amit K, Mandal Ujjal, Mukherjee Abhijit, Chatterjee Debashis, Mörth Carl-Magnus, Jacks Gunnar | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Drinking Water, Phosphates, Sulfates, Water Pollutants, Chemical, Manganese, Silicon Dioxide, Ammonia, Iron, Arsenic | IM | 22706147, S0048-9697(12)00710-3, 10.1016/j.scitotenv.2012.05.031 | Delineation of safe aquifer(s) that can be targeted by cheap drilling technology for tubewell (TW) installation becomes highly imperative to ensure access to safe and sustainable drinking water sources for the arsenic (As) affected population in Bengal Basin. This study investigates the potentiality of brown sand aquifers (BSA) as a safe drinking water source by characterizing its hydrogeochemical contrast to grey sand aquifers (GSA) within shallow depth (<70 m) over an area of 100 km(2) in Chakdaha Block of Nadia district, West Bengal, India. The results indicate that despite close similarity in major ion composition, the redox condition is markedly different in groundwater of the two studied aquifers. The redox condition in the BSA is delineated to be Mn oxy-hydroxide reducing, not sufficiently lowered for As mobilization into groundwater. In contrast, the enrichments of NH(4)(+), PO(4)(3-), Fe and As along with lower Eh in groundwater of GSA reflect reductive dissolution of Fe oxy-hydroxide coupled to microbially mediated oxidation of organic matter as the prevailing redox process causing As mobilization into groundwater of this aquifer type. In some portions of GSA the redox status even has reached to the stage of SO(4)(2-) reduction, which to some extent might sequester dissolved As from groundwater by co-precipitation with authigenic pyrite. Despite having low concentration of As in groundwater of the BSA the concentration of Mn often exceeds the drinking water guidelines, which warrants rigorous assessment of attendant health risk for Mn prior to considering mass scale exploitation of the BSA for possible sustainable drinking water supply. | Ammonia, Arsenic, Conservation of Natural Resources, Drinking Water, Environmental Monitoring, Factor Analysis, Statistical, Groundwater, India, Iron, Manganese, Phosphates, Silicon Dioxide, Sulfates, Water Pollutants, Chemical, Water Supply | null |
22,706,151 | 2012-12-14 | 2012-07-16 | 1872-7573 | Journal of ethnopharmacology | Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. | Muthuraman Arunachalam, Singh Nirmal | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Analgesics, Neuroprotective Agents, Plant Extracts, Saponins, Tumor Necrosis Factor-alpha | IM | 22706151, S0378-8741(12)00371-6, 10.1016/j.jep.2012.05.049 | Traditionally, Acorus calamus has been used for the treatment and management of headache, migraine, body ache and severe inflammatory pain in the Unani, Ayurveda and Indian system of medicine. | Acorus, Analgesics, Animals, Disease Models, Animal, Female, Hyperalgesia, Male, Neural Conduction, Neuralgia, Neuroprotective Agents, Phytotherapy, Plant Extracts, Rats, Rats, Wistar, Rhizome, Saponins, Sciatic Nerve, Tumor Necrosis Factor-alpha | null |
22,706,150 | 2012-12-18 | 2023-12-13 | 1872-7573 | Journal of ethnopharmacology | Protective effect of Heliotropium foertherianum (Boraginaceae) folk remedy and its active compound, rosmarinic acid, against a Pacific ciguatoxin. | Rossi Fanny, Jullian Valérie, Pawlowiez Ralph, Kumar-Roiné Shilpa, Haddad Mohamed, Darius H Taiana, Gaertner-Mazouni Nabila, Chinain Mireille, Laurent Dominique | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cinnamates, Depsides, Plant Extracts, ciguatoxin 1B (CTX 1B), Ciguatoxins | IM | 22706150, S0378-8741(12)00367-4, 10.1016/j.jep.2012.05.045 | Senescent leaves of Heliotropium foertherianum Diane & Hilger (Boraginaceae) are traditionally used in the Pacific region to treat Ciguatera Fish Poisoning. This plant contains rosmarinic acid that is known for its multiple biological activities. In the present study, H. foertherianum aqueous extract, rosmarinic acid and its derivatives were evaluated for their capacity to reduce the effect of ciguatoxins. | Animals, Cell Line, Tumor, Ciguatera Poisoning, Ciguatoxins, Cinnamates, Depsides, Heliotropium, Medicine, Traditional, Mice, Neuroblastoma, Pacific Islands, Phytotherapy, Plant Extracts, Plant Leaves, Rosmarinic Acid | null |
22,706,148 | 2012-12-14 | 2021-12-03 | 1872-7573 | Journal of ethnopharmacology | A Chinese herbal medicine, Gexia-Zhuyu Tang (GZT), prevents dimethylnitrosamine-induced liver fibrosis through inhibition of hepatic stellate cells proliferation. | Chen Jiun-Yu, Chen Hsiao-Ling, Cheng Ju-Chien, Lin Hung-Jen, Tung Yu-Tang, Lin Chia-Fan, Chen Chuan-Mu | eng | null | Journal Article | Actins, Collagen Type I, Collagen Type I, alpha 1 Chain, Drugs, Chinese Herbal, RNA, Messenger, smooth muscle actin, rat, Caspase 12, Caspase 3, Dimethylnitrosamine, Calcium | IM | 22706148, S0378-8741(12)00392-3, 10.1016/j.jep.2012.06.005 | Gexia-Zhuyu Tang (GZT), also called Gexiazhuyu decoction (GXZYD), is a traditional Chinese herbal medicine for chronic liver diseases such as cirrhosis and liver fibrosis. | Actins, Animals, Apoptosis, Calcium, Caspase 12, Caspase 3, Cell Line, Cell Proliferation, Collagen Type I, Collagen Type I, alpha 1 Chain, Dimethylnitrosamine, Drugs, Chinese Herbal, Hepatic Stellate Cells, Humans, Liver Cirrhosis, Male, Phytotherapy, RNA, Messenger, Rats, Rats, Sprague-Dawley, Ultrasonography | null |
22,706,149 | 2012-12-14 | 2016-11-25 | 1872-7573 | Journal of ethnopharmacology | Acanthopanax senticosus has a heme oxygenase-1 signaling-dependent effect on Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages. | Soo Kim Hye, Young Park Sun, Kyoung Kim Eun, Yeon Ryu Eun, Hun Kim Young, Park Geuntae, Joon Lee Sang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Inflammatory Agents, Cytokines, Lipopolysaccharides, Membrane Proteins, NF-E2-Related Factor 2, NF-kappa B, Nfe2l2 protein, mouse, Plant Extracts, STAT1 Transcription Factor, STAT3 Transcription Factor, Stat1 protein, mouse, Stat3 protein, mouse, Transcription Factor AP-1, Heme Oxygenase-1, Hmox1 protein, mouse, Mitogen-Activated Protein Kinases | IM | 22706149, S0378-8741(12)00393-5, 10.1016/j.jep.2012.06.006 | Acanthopanax senticosus (Rupr. & Maxim.) Harms (AS) has been used as a traditional medicine for the treatment of hypertension, rheumatism, ischemic heart disease, diabetes, and hepatitis in East Asia. This herb has been reported to possess anti-cancer, anti-diabetes, and anti-inflammatory properties. | Animals, Anti-Inflammatory Agents, Cell Line, Cytokines, Eleutherococcus, Heme Oxygenase-1, Lipopolysaccharides, Membrane Proteins, Mice, Mitogen-Activated Protein Kinases, NF-E2-Related Factor 2, NF-kappa B, Plant Extracts, STAT1 Transcription Factor, STAT3 Transcription Factor, Transcription Factor AP-1 | null |
22,706,152 | 2012-11-28 | 2012-07-31 | 1095-8541 | Journal of theoretical biology | Interspecies correlation for neutrally evolving traits. | Sagitov Serik, Bartoszek Krzysztof | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706152, S0022-5193(12)00285-8, 10.1016/j.jtbi.2012.06.008 | A simple way to model phenotypic evolution is to assume that after splitting, the trait values of the sister species diverge as independent Brownian motions. Relying only on a prior distribution for the underlying species tree (conditioned on the number, n, of extant species) we study the random vector (X(1),…,X(n)) of the observed trait values. In this paper we derive compact formulae for the variance of the sample mean and the mean of the sample variance for the vector (X(1),…,X(n)). The key ingredient of these formulae is the correlation coefficient between two trait values randomly chosen from (X(1),…,X(n)). This interspecies correlation coefficient takes into account not only variation due to the random sampling of two species out of n and the stochastic nature of Brownian motion but also the uncertainty in the phylogenetic tree. The latter is modeled by a (supercritical or critical) conditioned branching process. In the critical case we modify the Aldous-Popovic model by assuming a proper prior for the time of origin. | Computer Simulation, Genetic Drift, Models, Genetic, Quantitative Trait, Heritable, Regression Analysis, Software, Species Specificity | null |
22,706,153 | 2012-11-13 | 2012-07-17 | 1095-8541 | Journal of theoretical biology | Dispersal routes reconstruction and the minimum cost arborescence problem. | Hordijk Wim, Broennimann Olivier | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706153, S0022-5193(12)00284-6, 10.1016/j.jtbi.2012.06.007 | We show that the dispersal routes reconstruction problem can be stated as an instance of a graph theoretical problem known as the minimum cost arborescence problem, for which there exist efficient algorithms. Furthermore, we derive some theoretical results, in a simplified setting, on the possible optimal values that can be obtained for this problem. With this, we place the dispersal routes reconstruction problem on solid theoretical grounds, establishing it as a tractable problem that also lends itself to formal mathematical and computational analysis. Finally, we present an insightful example of how this framework can be applied to real data. We propose that our computational method can be used to define the most parsimonious dispersal (or invasion) scenarios, which can then be tested using complementary methods such as genetic analysis. | Algorithms, Centaurea, Geography, Introduced Species, Models, Biological, United States | null |
22,706,154 | 2012-10-16 | 2014-11-20 | 1464-0333 | Journal of environmental monitoring : JEM | Determination of contaminant levels and remediation efficacy in groundwater at a former in situ recovery uranium mine. | Borch Thomas, Roche Nicholas, Johnson Thomas E | eng | null | Journal Article | Water Pollutants, Chemical, Water Pollutants, Radioactive, Uranium | IM | 22706154, 10.1039/c2em30077j | There has been increasing interest in uranium mining in the United States via in situ recovery techniques. One of the main environmental concerns with in situ uranium mining is the potential for spreading groundwater contamination. There is a dearth of detailed analysis and information regarding the outcome of in situ uranium mine remediation to ascertain the environmental impacts. Regulatory measurements performed at a Wyoming in situ uranium mine were collected and analysed to ascertain the efficacy of remediation and potential long term environmental impact. Based on the measurements, groundwater sweeping followed by reverse osmosis (RO) treatment proved to be a highly efficient method of remediation. However, injection of a reductant in the form of H(2)S after groundwater sweeping and RO did not further reduce the aqueous concentration of U, Mn, or Fe. Low concentrations of target species at monitoring wells outside the mined area appear to indicate that in the long term, natural attenuation is likely to play a major role at reductively immobilizing residual (after remediation) concentrations of U(VI) thus preventing it from moving outside the mined area. Our analysis indicates the need for additional monitoring wells and sampling in conjunction with long term monitoring to better understand the impacts of the different remediation techniques. | Environmental Monitoring, Environmental Restoration and Remediation, Groundwater, Mining, Uranium, Water Pollutants, Chemical, Water Pollutants, Radioactive, Wyoming | null |
22,706,162 | 2012-12-26 | 2012-08-27 | 1567-7257 | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Molecular epidemiology and genetic history of European-type genotype 3 hepatitis E virus indigenized in the central region of Japan. | Nakano Tatsunori, Okano Hiroshi, Kobayashi Makoto, Ito Keiichi, Ohmori Shigeru, Nomura Tomoyuki, Kato Hideaki, Ayada Minoru, Nakano Yoko, Akachi Shigehiro, Sugimoto Kazushi, Fujita Naoki, Shiraki Katsuya, Takei Yoshiyuki, Takahashi Masaharu, Okamoto Hiroaki | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706162, S1567-1348(12)00217-1, 10.1016/j.meegid.2012.06.002 | In Mie prefecture in Japan, 12 cases of sporadic hepatitis E occurred from 2004 to 2011. Mie prefecture is located in the central region of Japan, far from the most prevalent regions of hepatitis E virus (HEV) infection in Japan, the north and northeastern part. These 12 cases did not have any common risk factors of HEV infection. We analyzed the molecular epidemiology of the cases in Mie prefecture. We obtained the nucleotide sequences of the HEV strains and analyzed them with the sequences of other HEV strains by phylogenetic and coalescent analyses. Japan-indigenous genotype 3 HEV strains were divided into two major subtypes, namely, 3a and 3b; one minor subtype, 3e; and a few other unassigned lineages. The Japan-indigenous subtype 3e strains were closely related to European subtype 3e HEV strains and were comparatively rare in Japan; however, eight strains of the 12 cases we examined belonged to subtype 3e, indicating a close phylogenetic relationship, despite the lack of common risk factors. Coalescent analyses indicated that the Mie 3e strains seemed to have intruded into Mie prefecture about 10 years ago. Sporadic acute hepatitis E cases caused by the 3e strains occurred consistently from 2004 to 2011 in Mie prefecture. This is the first report of unexpected persistent occurrence of hepatitis by the European-type genotype 3 HEV, subtype 3e, in a country outside of Europe. Phylogenetic and coalescent analyses traced the history of the indigenization of the Mie 3e strains from Europe. Because hepatitis E cases caused by 3e strains are relatively rare in Japan, molecular evolutionary analyses of HEV infection in Mie prefecture is important for preventing a future hepatitis endemic or epidemic by 3e strains in Japan. | Aged, Bayes Theorem, Disease Outbreaks, Evolution, Molecular, Female, Genotype, Hepatitis E, Hepatitis E virus, Humans, Japan, Likelihood Functions, Male, Markov Chains, Middle Aged, Models, Genetic, Molecular Epidemiology, Molecular Sequence Data, Monte Carlo Method, Open Reading Frames, Phylogeny, Phylogeography, Sequence Analysis, DNA | null |
22,706,165 | 2012-10-31 | 2014-11-20 | 1742-2051 | Molecular bioSystems | A metabonomics study of epilepsy in patients using gas chromatography coupled with mass spectrometry. | Wei Chunmin, Li Yi, Yao Han, Liu Huanjun, Zhang Xiumei, Guo Ruichen | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706165, 10.1039/c2mb25105a | Epilepsy is a cryptogenic neurological disorder characterized by recurrent seizures which may be precipitated by a variety of endogenous or exogenous factors, and usually occurs many months or years after a precipitating injury. Timely diagnosis and treatment at the early stage of epilepsy are very important for patients to prevent serious lesions and improve the quality of their life. In this study, the metabonomics approach based on the GC-MS technique, multivariate statistical analysis and the metabolism network analysis were applied to investigate metabolic changes in epileptic patients. The outcome of this study suggested that ten endogenous metabolites and five metabolism pathways were mainly involved and showed marked perturbations in epileptic patients. It not only enhances the understanding of the pathology of epilepsy, but also provides an experimental foundation for the therapeutic strategy of epilepsy. Furthermore, this work demonstrates the powerful predictive potential of the metabolic network analysis to neurological disease. | Adolescent, Adult, Child, Epilepsy, Gas Chromatography-Mass Spectrometry, Humans, In Vitro Techniques, Male, Metabolomics, Young Adult | null |
22,706,161 | 2013-02-21 | 2013-11-21 | 1095-8657 | Journal of structural biology | Unique water distribution of Langmuir-Blodgett versus classical crystals. | Pechkova Eugenia, Sivozhelezov Victor, Belmonte Luca, Nicolini Claudio | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Plant Proteins, Solvents, Water, thaumatin protein, plant, Ribonuclease, Pancreatic, Endopeptidase K, Thermolysin | IM | 22706161, S1047-8477(12)00171-2, 10.1016/j.jsb.2012.05.021 | Langmuir-Blodgett films when used as nanotemplates for crystallization often leads to marked changes in protein stability and structure. Earlier we found that stability of proteins is also correlated with aqueous surroundings in the crystals. Here we study the direct relationships between presence of LB nanotemplates and unique patterns of water molecules surrounding the protein, for four model proteins for which 3D structures are available, and where crystallization conditions for each protein are the same except the presence of LB nanotemplate. Shape of frequency distribution of volumes occupied by water molecules were analyzed. They were found to be different between "classical" samples of different proteins, but surprisingly quite similar for LB samples. Volumes occupied by each water molecule as the function of the distance of the given molecule from the protein surface were studied. Introduction of LB film leads to appearance of water molecules close to protein surface but occupying large volumes. These findings confirm earlier experimental findings on the role of water molecules in determining protein stability and thereby pointing to water as a possible candidate for differences apparent in LB crystal stability against radiation. | Crystallization, Crystallography, X-Ray, Endopeptidase K, Nanostructures, Plant Proteins, Protein Stability, Ribonuclease, Pancreatic, Solvents, Thermolysin, Water | null |
22,706,156 | 2012-11-13 | 2012-12-07 | 1532-5725 | Journal of the American Psychiatric Nurses Association | Staffing yes, but competency, too. | Fitzgerald Michael | eng | null | Letter | null | IM | 22706156, 18/3/145, 10.1177/1078390312446693 | null | Clinical Competence, Humans, Mental Disorders, Nursing Staff, Hospital, Personnel Staffing and Scheduling, Psychiatric Nursing, United States | null |
22,706,157 | 2012-11-13 | 2012-12-07 | 1532-5725 | Journal of the American Psychiatric Nurses Association | A clinical translation of the research article titled "altruism in survivors of sexual violence: the typology of helping others". | Willis Danny G, Beeber Linda S | eng | null | Journal Article | null | null | 22706157, 18/3/156, 10.1177/1078390312448821 | null | null | null |
22,706,158 | 2012-11-13 | 2018-12-01 | 1532-5725 | Journal of the American Psychiatric Nurses Association | Characteristics of patients with histories of multiple seclusion and restraint events during a single psychiatric hospitalization. | Taylor Karin, Mammen Kristine, Barnett Shannon, Hayat Matt, Dosreis Susan, Gross Deborah | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706158, 18/3/159, 10.1177/1078390311432167 | This descriptive, retrospective study examined patient and event characteristics associated with multiple seclusion and restraint (SR). | Adult, Aggression, Baltimore, Behavior Control, Delirium, Episode of Care, Female, Hospitals, Psychiatric, Humans, Inpatients, Male, Mental Disorders, Patient Isolation, Restraint, Physical, Retrospective Studies, Risk Assessment, Risk Factors, Sex Distribution | null |
22,706,160 | 2012-09-07 | 2022-04-08 | 1476-4679 | Nature cell biology | USP4 is regulated by AKT phosphorylation and directly deubiquitylates TGF-β type I receptor. | Zhang Long, Zhou FangFang, Drabsch Yvette, Gao Rui, Snaar-Jagalska B Ewa, Mickanin Craig, Huang Huizhe, Sheppard Kelly-Ann, Porter Jeff A, Lu Chris X, ten Dijke Peter | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Oncogene Proteins, Protein Kinase Inhibitors, Proto-Oncogene Proteins, Receptors, Transforming Growth Factor beta, Transforming Growth Factor beta, USP4 protein, human, Usp4 protein, mouse, Protein Serine-Threonine Kinases, Proto-Oncogene Proteins c-akt, Receptor, Transforming Growth Factor-beta Type I, Ubiquitin Thiolesterase, Ubiquitin-Specific Proteases | IM | 22706160, ncb2522, 10.1038/ncb2522, 21448580, 20096447, 12717440, 9335507, 19030025, 19855013, 8843198, 12070302, 2140528, 19114990, 10969078, 16186809, 21111230, 22262844, 19648010, 22399812, 21947082, 20495575, 15459392, 21925315, 16472766, 15104092, 19615732, 12621150, 14647420, 10521335, 18371439, 11016919, 18978814, 16818646, 21331078, 21411632, 12151385, 19153598, 15048128, 22337149, 21393860, 16325574, 21522127, 11158580, 18662538, 15968639, 22344298, 10458166, 19945376, 20037158, 17604717, 21884813, 15196956, 9799433, 12842083, 10587642, 22335355, 22374800, 19345189, 11163210, 22029577, 20725033 | The stability and membrane localization of the transforming growth factor-β (TGF-β) type I receptor (TβRI) determines the levels of TGF-β signalling. TβRI is targeted for ubiquitylation-mediated degradation by the SMAD7-SMURF2 complex. Here we performed a genome-wide gain-of-function screen and identified ubiquitin-specific protease (USP) 4 as a strong inducer of TGF-β signalling. USP4 was found to directly interact with TβRI and act as a deubiquitylating enzyme, thereby controlling TβRI levels at the plasma membrane. Depletion of USP4 mitigates TGF-β-induced epithelial to mesenchymal transition and metastasis. Importantly, AKT (also known as protein kinase B), which has been associated with poor prognosis in breast cancer, directly associates with and phosphorylates USP4. AKT-mediated phosphorylation relocates nuclear USP4 to the cytoplasm and membrane and is required for maintaining its protein stability. Moreover, AKT-induced breast cancer cell migration was inhibited by USP4 depletion and TβRI kinase inhibition. Our results uncover USP4 as an important determinant for crosstalk between TGF-β and AKT signalling pathways. | Animals, Breast Neoplasms, Cell Membrane, Cell Movement, Enzyme Stability, Epithelial-Mesenchymal Transition, Female, Gene Expression Regulation, HEK293 Cells, HeLa Cells, Humans, Mice, Mice, Knockout, Mutation, Neoplasm Invasiveness, Oncogene Proteins, Phosphorylation, Protein Kinase Inhibitors, Protein Serine-Threonine Kinases, Protein Transport, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-akt, RNA Interference, Receptor, Transforming Growth Factor-beta Type I, Receptors, Transforming Growth Factor beta, Signal Transduction, Time Factors, Transfection, Transforming Growth Factor beta, Ubiquitin Thiolesterase, Ubiquitin-Specific Proteases, Ubiquitination, Zebrafish | null |
22,706,166 | null | 2022-04-09 | 1179-8467 | Substance abuse and rehabilitation | Social capital and cigarette smoking among Latinos in the United States. | Li Shijian, Horner Pilar, Delva Jorge | eng | P60 MD000538 (NIMHD NIH HHS, United States); P60 MD000538-01 (NIMHD NIH HHS, United States); U58 DP001022 (NCCDPHP CDC HHS, United States) | Journal Article | null | null | 22706166, 10.2147/SAR.S31164, PMC3374601, NIHMS370832, 19008791, 16971030, 14759942, 16809587, 15719532, 10691754, 15347534, 19427087, 21330593, 18407007, 9784089, 16809581, 8882838, 18994664, 19616270, 19299683, 15719530, 18705683, 16615038, 10600066, 15514238, 15970227, 19672330, 22013026, 15252073, 17049701, 18482856, 16860857, 20693233, 22706023 | This paper presents the results of analyses conducted to examine if social capital indicators were associated with current cigarette smoking and with quitting smoking among a national representative sample of Latinos living in the United States. Data are from 2540 Mexican Americans, Puerto Ricans, Cuban Americans, and Other Latinos who participated in the National Latino and Asian American Survey. A significant inverse association between neighborhood cohesion and current smoking, and a positive association with quitting smoking, were found only among Mexican Americans. No other significant associations were found except for family conflict being associated with higher odds of current smoking with Cuban Americans. Implications of these findings are discussed to unravel the differences in social capital and smoking behaviors among Latino populations. | null | null |
22,706,159 | 2012-11-13 | 2012-12-07 | 1532-5725 | Journal of the American Psychiatric Nurses Association | APNA's strategic plan: looking into the future. | Nadler-Moodie Marlene | eng | null | Journal Article | null | IM | 22706159, 18/3/190, 10.1177/1078390312449044 | null | Humans, Organizational Objectives, Psychiatric Nursing, Societies, Nursing, United States | null |
22,706,155 | 2012-11-13 | 2012-12-07 | 1532-5725 | Journal of the American Psychiatric Nurses Association | Staffing inpatient psychiatric units. | Polacek Michael | eng | null | Letter | null | IM | 22706155, 18/3/144, 10.1177/1078390312446224 | null | Hospitals, Psychiatric, Humans, Mental Disorders, Nursing Staff, Hospital, Patient Safety, Personnel Staffing and Scheduling, Psychiatric Nursing, United States | null |
22,706,167 | 2013-09-03 | 2012-11-28 | 1473-5709 | European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) | Probiotics enhance the clearance of human papillomavirus-related cervical lesions: a prospective controlled pilot study. | Verhoeven Veronique, Renard Nathalie, Makar Amin, Van Royen Paul, Bogers John-Paul, Lardon Filip, Peeters Marc, Baay Marc | eng | null | Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22706167, 10.1097/CEJ.0b013e328355ed23 | Probiotics have been proposed for a number of urogenital infectious conditions. In this study, we examine a possible effect on human papillomavirus (HPV)-related precancerous lesions in cervical cytology. We conducted a prospective controlled pilot study, in which 54 women with an HPV+low-grade squamous intraepithelial lesion diagnosis in their PAP smear were followed for 6 months. The intervention group consumed a daily probiotic drink during the study period; the control group received no treatment, according to common care policy. Outcome measures were the control PAP smear and HPV status after 6 months. Probiotic users had a twice as high chance of clearance of cytological abnormalities (60 vs. 31%, P=0.05). HPV was cleared in 19% of control patients versus 29% of probiotic users (P=0.41). This exploratory pilot study suggests that the probiotic studied promotes the clearance of HPV-related cytological abnormalities. If confirmed, this would represent an entirely new option to manage cervical cancer precursors. | Adult, Female, Humans, Neoplasms, Squamous Cell, Papillomaviridae, Papillomavirus Infections, Pilot Projects, Probiotics, Prospective Studies, Up-Regulation, Uterine Cervical Neoplasms, Young Adult | null |
22,706,168 | 2012-09-21 | 2021-10-21 | 1879-3185 | Toxicology | Role of leukotrienes in N-(3,5-dichlorophenyl)succinimide (NDPS) and NDPS metabolite nephrotoxicity in male Fischer 344 rats. | Rankin Gary O, Hong Suk K, Anestis Dianne K, Ball John G, Valentovic Monica A, Graffeo Vincent A | eng | P20 RR016477 (NCRR NIH HHS, United States); R01 DK031210 (NIDDK NIH HHS, United States); RR016477 (NCRR NIH HHS, United States); DK31210 (NIDDK NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Acetophenones, Fungicides, Industrial, Leukotriene A4, Leukotrienes, Receptors, Leukotriene, Succinates, Succinimides, Tetrazoles, N-(3,5-dichlorophenyl)-2-hydroxysuccinimide, N-(3,5-dichlorophenyl)succinimide, N-(3,5-dichlorophenyl)-2-hydroxysuccinamic acid, LY 171883, leukotriene D4 receptor, Diethylcarbamazine | IM | 22706168, S0300-483X(12)00220-X, 10.1016/j.tox.2012.06.003, PMC3412395, NIHMS390805, 9925801, 10593507, 942051, 7709745, 3975935, 7624885, 2175681, 8497867, 2817583, 15204747, 17310080, 18261606, 10446122, 21224494, 2356574, 7089982, 1519601, 1771640, 7521568, 20952510, 21472229, 10348796, 3206523, 11516516, 3603574, 20388065, 16343055, 1680251, 6311237, 15962934, 3004501, 10199580, 18407308, 2127366, 10321901, 2009578, 1160343, 10844628, 6860996, 6710545, 15371242, 1882389, 21245029 | The agricultural fungicide N-(3,5-dichlorophenyl)succinimide (NDPS) can induce marked nephrotoxicity in rats following a single intraperitoneal (ip) administration of 0.4mmol/kg or greater. Although NDPS induces direct renal proximal tubular toxicity, a role for renal vascular effects may also be present. The purpose of this study was to examine the possible role of vasoconstrictor leukotrienes in NDPS and NDPS metabolite nephrotoxicity. Male Fischer 344 rats (4 rats/group) were administered diethylcarbamazine (DEC; 250 or 500mg/kg, ip), an inhibitor of LTA(4) synthesis, 1h before NDPS (0.4mmol/kg, ip), N-(3,5-dichlorophenyl)-2-hydroxysuccinimide (NDHS, 0.1, 0.2, or 0.4mmol/kg, ip), or N-(3,5-dichlorophenyl)-2-hydroxysuccinamic acid (2-NDHSA, 0.1mmol/kg, ip) or vehicle. In a separate set of experiments, the LTD(4) receptor antagonist LY171883 (100mg/kg, po) was administered 0.5h before and again 6h after NDHS (0.1mmol/kg, ip) or 2-NDHSA (0.1mmol/kg, ip) or vehicle. Renal function was monitored for 48h post-NDPS or NDPS metabolite. DEC markedly reduced the nephrotoxicity induced by NDPS and its metabolites, while LY171883 treatments provided only partial attenuation of NDHS and 2-NDHSA nephrotoxicity. These results suggest that leukotrienes contribute to the mechanisms of NDPS nephrotoxicity. | Acetophenones, Animals, Diethylcarbamazine, Fungicides, Industrial, Injections, Intraperitoneal, Kidney, Leukotriene A4, Leukotrienes, Male, Rats, Rats, Inbred F344, Receptors, Leukotriene, Succinates, Succinimides, Tetrazoles | null |
22,706,169 | 2012-10-05 | 2018-12-01 | 1879-3185 | Toxicology | Opposed arsenite-mediated regulation of p53-survivin is involved in neoplastic transformation, DNA damage, or apoptosis in human keratinocytes. | Li Yuan, Jiang Rongrong, Zhao Yue, Xu Yuan, Ling Min, Pang Ying, Shen Lu, Zhou Yun, Zhang Jianping, Zhou Jianwei, Wang Xinru, Liu Qizhan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Arsenites, BIRC5 protein, human, HSP70 Heat-Shock Proteins, HSPA9 protein, human, Inhibitor of Apoptosis Proteins, Mitochondrial Proteins, Sodium Compounds, Survivin, Tumor Suppressor Protein p53, sodium arsenite | IM | 22706169, S0300-483X(12)00221-1, 10.1016/j.tox.2012.06.004 | Biphasic dose-response relationship induced by environmental agents is often characterized with the effect of low-dose stimulation and high dose inhibition. Some studies showed that arsenite may induce cell proliferation and apoptosis via biphasic dose-response relationship in human cells; however, mechanisms underlying this phenomenon are not well understood. Our present study shows that, for human keratinocytes (HaCaT) cells, a low concentration of arsenite activates extracellular signal-regulated kinases (ERKs), which leads to up-regulation of nuclear factor κB (NF-κB) binding to DNA and to elevated, NF-κB-dependent expression of mot-2 (a p53 inhibitor) and survivin (an inhibitor of apoptosis). Activation of p53 is blocked, and neoplastic transformation is enhanced. Inhibition of ERKs reduces cell proliferation and neoplastic transformation. In contrast, a high concentration of arsenite activates c-Jun N-terminal kinases (JNKs), positive regulators of p53, by binding to p53 and preventing its murine double minute 2 (mdm2)-mediated degradation. The elevated levels of p53 lead to repair of DNA damage and apoptosis. Inhibition of JNKs increases DNA damage but decreases apoptosis. By identifying a mechanism whereby ERKs and JNKs-mediated regulation of the p53-survivin signal pathway is involved in the biphasic effects of arsenite on human keratinocytes, our data expand understanding of arsenite-induced cell proliferation, neoplastic transformation, DNA damage, and apoptosis. | Animals, Apoptosis, Arsenites, Blotting, Southwestern, Blotting, Western, Cell Culture Techniques, Cell Line, Cell Survival, Cell Transformation, Neoplastic, DNA Damage, Dose-Response Relationship, Drug, HSP70 Heat-Shock Proteins, Humans, Immunoprecipitation, Inhibitor of Apoptosis Proteins, Keratinocytes, MAP Kinase Signaling System, Mice, Mice, Nude, Mitochondrial Proteins, Neoplasms, Real-Time Polymerase Chain Reaction, Sodium Compounds, Survivin, Tumor Suppressor Protein p53 | null |
22,706,170 | 2012-12-03 | 2017-11-16 | 1095-9319 | Microvascular research | Characterization and comparison of embryonic stem cell-derived KDR+ cells with endothelial cells. | Sun Xuan, Cheng Lamei, Duan Huaxin, Lin Ge, Lu Guangxiu | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Antigens, CD, Biomarkers, Cadherins, Lipoproteins, LDL, Plant Lectins, Platelet Endothelial Cell Adhesion Molecule-1, Ulex europaeus lectins, acetyl-LDL, cadherin 5, von Willebrand Factor, Vascular Endothelial Growth Factor Receptor-2 | IM | 22706170, S0026-2862(12)00114-8, 10.1016/j.mvr.2012.06.003 | Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both cord blood endothelial progenitor cells (CBEPCs) and human umbilical vein endothelial cells (HUVECs) in vitro. The results showed that isolated embryonic KDR+ cells (EC-KDR+) display characteristics that were similar to CBEPCs and HUVECs. EC-KDR+, CBEPCs and HUVECs all expressed CD31 and CD144, incorporated DiI-Ac-LDL, bound UEA1 lectin, and were able to form tube-like structures on Matrigel. Compared with CBEPCs and HUVECs, the expression level of endothelial progenitor cell markers such as CD133 and KDR in EC-KDR+ was significantly higher, while the mature endothelial marker vWF was lowly expressed in EC-KDR+. In summary, the study showed that EC-KDR+ are primitive endothelial-like progenitors and might be a potential source for therapeutic vascular regeneration and tissue engineering. | Antigens, CD, Biological Transport, Biomarkers, Cadherins, Cell Differentiation, Cell Line, Cell Proliferation, Embryonic Stem Cells, Endothelial Cells, Fetal Blood, Human Umbilical Vein Endothelial Cells, Humans, Lipoproteins, LDL, Neovascularization, Physiologic, Phenotype, Plant Lectins, Platelet Endothelial Cell Adhesion Molecule-1, Time Factors, Vascular Endothelial Growth Factor Receptor-2, von Willebrand Factor | null |
22,706,172 | 2015-05-01 | 2022-03-21 | 1537-453X | American journal of clinical oncology | Radiation therapy in the management of patients with limited brain metastases. | Dhakal Sughosh, Peterson Carl R, Milano Michael T | eng | null | Journal Article, Meta-Analysis, Review | null | IM | 22706172, 10.1097/COC.0b013e3182546807 | Brain metastases are the most common form of intracranial tumor in adults and an increasingly important cause of morbidity and mortality. Rising incidence is attributed to advanced radiographic imaging and prolonged survival due to improvements in cancer therapy (including systemic therapies) that are not as effective in treating intracranial disease. Standard treatment options for brain metastases include resection, whole-brain radiation therapy (WBRT), stereotactic radiosurgery, or a combination of these modalities. Most patients with brain metastases receive some form of radiation therapy during the course of their illness, and for the majority of them, the prognosis is poor and WBRT remains the standard. However, within this very diverse patient population, subgroups exist in which prolonged survival is possible. In recent years, several randomized controlled trials have clearly demonstrated the efficacy of stereotactic radiosurgery in well-selected patients. This, along with an increased recognition of the late neurocognitive effects of WBRT, has led many to question the role of upfront WBRT in patients with limited intracranial metastases. In this review, we summarize the evolving role of radiotherapy in the management of brain metastases and then discuss the issues related to neurotoxicity from radiation and future areas of investigation. | Brain Neoplasms, Humans, Prognosis, Radiosurgery, Radiotherapy, Adjuvant | null |
22,706,171 | 2013-11-14 | 2018-12-02 | 1537-453X | American journal of clinical oncology | Prospective and comparative evaluation of the toxicity of adjuvant concurrent chemoradiotherapy after neoadjuvant chemotherapy for breast cancer. | Marchand Virginie, Angelergues Antoine, Gobaux Vanessa, Hajage David, Kirova Youlia M, Campana François, Dendale Rémi, Reyal Fabien, Pierga Jean-Yves, Fourquet Alain, Bollet Marc A | eng | null | Comparative Study, Journal Article | Vinblastine, Vinorelbine, Fluorouracil | IM | 22706171, 10.1097/COC.0b013e31825466a6 | The lack of pathologic breast cancer response to neoadjuvant chemotherapy (NCT), a negative prognostic factor, has prompted the addition of chemotherapy to adjuvant radiotherapy. This study aims to investigate prospectively the toxicities of adjuvant concurrent chemoradiotherapy versus radiotherapy alone. | Adult, Aged, Antineoplastic Combined Chemotherapy Protocols, Breast Neoplasms, Case-Control Studies, Chemoradiotherapy, Chemoradiotherapy, Adjuvant, Female, Fluorouracil, Follow-Up Studies, Humans, Middle Aged, Neoadjuvant Therapy, Neoplasm Grading, Neoplasm Staging, Prognosis, Prospective Studies, Survival Rate, Vinblastine, Vinorelbine | null |
22,706,173 | 2014-10-23 | 2018-12-02 | 1537-453X | American journal of clinical oncology | Is there an increase in genitourinary toxicity in patients treated with transurethral resection of the prostate and radiotherapy? A systematic review. | Ishiyama Hiromichi, Hirayama Takahiro, Jhaveri Pavan, Satoh Takefumi, Paulino Arnold C, Xu Bo, Butler Edward Brian, Teh Bin S | eng | null | Journal Article, Review, Systematic Review | null | IM | 22706173, 10.1097/COC.0b013e3182546821 | Transurethral resection of the prostate (TURP) is considered by some as a risk factor for genitourinary (GU) toxicity after radiotherapy (RT). However, there are conflicting results regarding the interaction between RT and TURP with respect to GU toxicity. The purpose of this report is to review the published data concerning TURP before or after RT and its effect on urinary complication. | Brachytherapy, Constriction, Pathologic, Humans, Laser Therapy, Male, Prostatic Hyperplasia, Prostatic Neoplasms, Radiation Injuries, Radiotherapy, Adjuvant, Risk Factors, Severity of Illness Index, Time Factors, Transurethral Resection of Prostate, Urethra, Urination Disorders, Urogenital System | null |
22,706,176 | 2013-11-14 | 2013-11-21 | 1537-453X | American journal of clinical oncology | The use of Fluorouracil (5-FU) and leucovorin in women with heavily pretreated advanced ovarian carcinoma. | Peled Yoav, Levavi Hanoch, Krissi Haim, Weill Yehudah, Sabah Gad, Eitan Ram | eng | null | Journal Article | Leucovorin, Fluorouracil | IM | 22706176, 10.1097/COC.0b013e3182549399 | Women suffering from recurrent platinum-resistant ovarian carcinoma go through several lines of chemotherapy, but eventually fail all conventional chemotherapy options. After failing multiple other regimens, we offer patients fluorouracil (5-FU) in a weekly regimen with leucovorin. For those women who failed to react to multiple lines of treatment, 5-FU has been shown to be a reasonable option with reported response rates of 10% to 33%. We report our experience with 5-FU+leucovorin in this patient population. | Adenocarcinoma, Mucinous, Aged, Antineoplastic Combined Chemotherapy Protocols, Carcinoma, Papillary, Cystadenocarcinoma, Serous, Endometrial Neoplasms, Female, Fluorouracil, Follow-Up Studies, Humans, Leucovorin, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Ovarian Neoplasms, Prognosis, Retrospective Studies, Survival Rate | null |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.