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22,705,856 | 2013-01-23 | 2012-09-05 | 1095-8630 | Journal of environmental management | Social factors and private benefits influence landholders' riverine restoration priorities in tropical Australia. | Januchowski-Hartley Stephanie Renee, Moon Katie, Stoeckl Natalie, Gray Sally | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705856, S0301-4797(12)00269-1, 10.1016/j.jenvman.2012.05.011 | Private land conservation is an essential component of conservation that requires organizing both protection and restoration actions accordingly. Yet private land conservation programs are often formulated to generate public benefits, with inadequate consideration of costs or benefits to private landholders. Landholders' willingness to participate in conservation programs depends on a complex set of social factors, and the benefits they expect from participation. However, these two attributes are commonly evaluated independent of one another. We addressed this limitation through interviews aimed at determining landholders': 1) willingness to participate in restoration programs; 2) barriers to participation; 3) prioritization of proposed riverine restoration actions; 4) expected public or private benefits for undertaking proposed riverine restoration actions; and 5) most preferred incentive for undertaking proposed restoration actions on their land. Our results revealed four main findings. First, landholders stated that biases towards ecological rather than production outcomes, impractical programs, and government mistrust (structural factors) were the major barriers that prevented them from participating in riverine restoration on their land. Second, private benefits influenced landholders' willingness to engage riverine restoration. Third, 'a sense of stewardship and improved landscape aesthetics' (an internal factor) was the most commonly reported private benefit. Fourth, the most preferred incentives for high priority restoration actions were cash for on-ground works, extension and community recognition. We highlight the importance of designing private land conservation programs that align with landholders' priorities and deliver public benefits. | Attitude, Conservation of Natural Resources, Humans, Private Sector, Queensland, Socioeconomic Factors | null |
22,705,857 | 2013-01-23 | 2022-12-07 | 1095-8630 | Journal of environmental management | Characteristics of treated effluents and their potential applications for producing concrete. | Noruzman Ainul Haezah, Muhammad Bala, Ismail Mohammad, Abdul-Majid Zaiton | eng | null | Evaluation Study, Journal Article | Industrial Waste, Plant Oils, Sewage, Waste Water, Palm Oil | IM | 22705857, S0301-4797(12)00278-2, 10.1016/j.jenvman.2012.05.019 | Conservation and preservation of freshwater is increasingly becoming important as the global population grows. Presently, enormous volumes of freshwater are used to mix concrete. This paper reports experimental findings regarding the feasibility of using treated effluents as alternatives to freshwater in mixing concrete. Samples were obtained from three effluent sources: heavy industry, a palm-oil mill and domestic sewage. The effluents were discharge into public drain without danger to human health and natural environment. Chemical compositions and physical properties of the treated effluents were investigated. Fifteen compositional properties of each effluent were correlated with the requirements set out by the relevant standards. Concrete mixes were prepared using the effluents and freshwater to establish a base for control performance. The concrete samples were evaluated with regard to setting time, workability, compressive strength and permeability. The results show that except for some slight excesses in total solids and pH, the properties of the effluents satisfy the recommended disposal requirements. Two concrete samples performed well for all of the properties investigated. In fact, one sample was comparatively better in compressive strength than the normal concrete; a 9.4% increase was observed at the end of the curing period. Indeed, in addition to environmental conservation, the use of treated effluents as alternatives to freshwater for mixing concrete could save a large amount of freshwater, especially in arid zones. | Conservation of Natural Resources, Construction Materials, Fresh Water, Industrial Waste, Palm Oil, Plant Oils, Sewage, Waste Disposal, Fluid, Wastewater, Water Pollution, Chemical | null |
22,705,859 | 2013-01-23 | 2022-03-18 | 1095-8630 | Journal of environmental management | Anaerobic digestion of pineapple pulp and peel in a plug-flow reactor. | Namsree Pimjai, Suvajittanont Worakrit, Puttanlek Chureerat, Uttapap Dudsadee, Rungsardthong Vilai | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | Biofuels, Industrial Waste, Methane | IM | 22705859, S0301-4797(12)00275-7, 10.1016/j.jenvman.2012.05.017 | The objective of this research was to study the production of biogas by using pineapple pulp and peel, the by-products from fruit processing plants, in a plug-flow reactor (17.5 L total volume). The effects of feed concentration, total solids (TS) and hydraulic retention time (HRT) on degradation of the waste were investigated. The increase of pineapple pulp and peel of 2% (wt/vol) at HRT 7 d to 4% (wt/vol) at HRT 10 d showed increases in biogas production rate, biogas yield and methane yield - from 0.12 v/v-d, 0.26 m(3)/kg COD removed and 0.11 m(3)/kg COD removed, with COD removal at 64.1%, to 0.25 v/v-d, 0.43 m(3)/kg COD removed and 0.14 m(3)/kg COD removed, with COD removal at 60.41%. The methanogenic fermentation was more active in the middle and final parts of the reactor. The recirculation of fermentation effluent at 40% (vol/vol) of the working volume into the reactor could increase the biogas production rate and biogas yield up to 52% and 12%, respectively. The results showed technological potential for waste treatment of pineapple pulp and peel in a plug-flow reactor. | Anaerobiosis, Ananas, Biofuels, Bioreactors, Fermentation, Food-Processing Industry, Industrial Waste, Methane, Thailand, Time Factors, Waste Management | null |
22,705,858 | 2013-01-23 | 2015-11-23 | 1095-8630 | Journal of environmental management | A comparative study on the degradation of RB-19 dye in an aqueous medium by advanced oxidation processes. | Guimarães José Roberto, Maniero Milena Guedes, Nogueira de Araújo Renata | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Anthraquinones, Coloring Agents, Fenton's reagent, Water Pollutants, Chemical, Hydrogen Peroxide, Iron, Remazol Brilliant Blue R | IM | 22705858, S0301-4797(12)00279-4, 10.1016/j.jenvman.2012.05.020 | The effectiveness of photolysis (UV), peroxidation (H(2)O(2)), peroxidation combined with UV light (UV/H(2)O(2)), Fenton reagent (H(2)O(2)/Fe(2+)), and the photo-Fenton process (H(2)O(2)/Fe(2+)/UV) at degrading the textile dye Reactive Blue 19 was evaluated. The efficiency of the photo-Fenton process for degrading raw textiles and biologically pre-treated effluents was also evaluated. H(2)O(2) (100-800 mg L(-1)) and UV light did not degrade dye when used separately. The UV/H(2)O(2) process was effective but slow: 91% of dye degraded within 3 h of reaction at a concentration of 500 mg L(-1) H(2)O(2). Fenton reagent reduced dissolved organic carbon by 36.8% and color was reduced by >98% within a few minutes of reaction. The photo-Fenton process was the most efficient, reducing 94.5% of dissolved organic carbon and 99.4% of color. The combination of a biological system and the photo-Fenton process degraded a high level of textile effluent degradation, reducing dissolved organic carbon by 88%, color by 85%, chemical oxygen demand by 80%, and biochemical oxygen demand by 93%. | Anthraquinones, Coloring Agents, Environmental Restoration and Remediation, Hydrogen Peroxide, Iron, Oxidation-Reduction, Photolysis, Time Factors, Ultraviolet Rays, Water Pollutants, Chemical, Water Pollution, Chemical | null |
22,705,860 | 2012-12-07 | 2013-11-21 | 1872-7549 | Behavioural brain research | Chronic exposure to mercuric chloride during gestation affects sensorimotor development and later behaviour in rats. | Chehimi Latifa, Roy Vincent, Jeljeli Mustapha, Sakly Mohsen | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Mercuric Chloride | IM | 22705860, S0166-4328(12)00418-4, 10.1016/j.bbr.2012.06.005 | The current study was performed to assess the effects of inorganic mercury (mercuric chloride - HgCl(2)) on the development of offsprings from intoxicated-mother during pregnancy. In this respect, pregnant rats were chronically treated with HgCl(2) at 50 ppm (Hg50) and 100 ppm (Hg100) in drinking water. After parturition, maternal behaviour was recorded during 30 min at 1st to 6th postnatal day (Pnd). The development of their offspring was studied during the first 17 days after birth. Sensorimotor development of pups was measured by different tests: rooting reflex, vibrissae placing response, righting reflex, negative geotaxis, suspension test and rotating grid. Two month after birth, the anxiety of offspring was tested using the elevated plus maze test. Our results indicate that mercury treatment significantly reduced the nursing and increased the time out the nest or drinking and eating. We also showed that prenatal exposure to HgCl(2) decreased weight gain. Importantly, the rooting reflex, the development of the vibrissae placing response, the righting reflex, the grip strength and the negative geotaxis behaviour were delayed in the offspring of dams treated with Hg50, the delay being more severe with Hg100. We also found a decrease in anxiety in adulthood. Cross-fostering test support the direct toxic effects of mercury. | Animals, Animals, Newborn, Behavior, Animal, Body Weight, Cognition, Female, Male, Maternal Behavior, Maternal Exposure, Mercuric Chloride, Motor Activity, Pregnancy, Prenatal Exposure Delayed Effects, Rats, Rats, Wistar, Reflex | null |
22,705,864 | 2012-11-30 | 2013-11-21 | 1879-1026 | The Science of the total environment | Analysis of CO2 daily cycle in the low atmosphere at a rural site. | Pérez Isidro A, Sánchez M Luisa, García M Ángeles, Pardo Nuria | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Carbon Dioxide | IM | 22705864, S0048-9697(12)00749-8, 10.1016/j.scitotenv.2012.05.067 | Directional analysis procedures were extended to study the CO(2) daily cycle in the low atmosphere at a rural site. Weighted average, asymmetric circular functions, and conditional probability curves were applied to concentrations recorded at three levels near surface. Wind speed and potential temperature gradient were the meteorological variables used to establish a link to CO(2). The highest weighted concentration was obtained for wind speed below 4 m s(-1) and for potential temperature gradient above 0.05 °C m(-1). Asymmetric circular functions were fitted to selected percentiles, the generalized von Mises function providing the lowest RMSE. Slight differences among levels were observed in the transition between day and night, and higher gradients were observed during the night. Four intervals were proposed when concentrations were binned by intervals of meteorological variables. Wind speed was only relevant to separate intermediate concentrations. Finally, conditional probability curves provided differences between the highest and lowest levels in up to 20% of cases at 20-22 GMT. | Atmosphere, Carbon Dioxide, Environmental Monitoring, Models, Statistical, Spain, Weather, Wind | null |
22,705,861 | 2012-11-05 | 2012-07-11 | 1473-5725 | Blood pressure monitoring | The effect of physician presence on blood pressure. | Hong Dezhi, Su Hai, Li Juxiang, Xu Jinsong, Peng Qiang, Yang Qing, Wang Jiwei, Cheng Xiaoshu | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705861, 10.1097/MBP.0b013e328355fe14 | To evaluate the effect of physician presence on blood pressure (BP) and the influencing factors. | Blood Pressure, Blood Pressure Determination, Blood Pressure Monitors, Female, Humans, Male, Middle Aged, Sex Factors, White Coat Hypertension | null |
22,705,862 | 2012-12-07 | 2015-11-19 | 1758-4469 | Tree physiology | Photosynthetic traits around budbreak in pre-existing needles of Sakhalin spruce (Picea glehnii) seedlings grown under elevated CO2 concentration assessed by chlorophyll fluorescence measurements. | Kitao Mitsutoshi, Tobita Hiroyuki, Utsugi Hajime, Komatsu Masabumi, Kitaoka Satoshi, Maruyama Yutaka, Koike Takayoshi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Photosystem II Protein Complex, Chlorophyll, Carbon Dioxide, Starch | IM | 22705862, tps048, 10.1093/treephys/tps048 | To assess the effects of elevated CO(2) concentration ([CO(2)]) on the photosynthetic properties around spring budbreak, we monitored the total leaf sugar and starch content, and chlorophyll fluorescence in 1-year-old needles of Sakhalin spruce (Picea glehnii Masters) seedlings in relation to the timing of budbreak, grown in a phytotron under natural daylight at two [CO(2)] levels (ambient: 360 μmol mol(-1) and elevated: 720 μmol mol(-1)). Budbreak was accelerated by elevated [CO(2)] accompanied with earlier temporal declines in the quantum yield of PSII electron transport (Φ(PSII)) and photochemical quenching (q(L)). Plants grown under elevated [CO(2)] showed pre-budbreak leaf starch content twice as high with no significant difference in Φ(PSII) from ambient-CO(2)-grown plants when compared at the same measurement [CO(2)], i.e., 360 or 720 μmol mol(-1), suggesting that the enhanced pre-budbreak leaf starch accumulation might not cause down-regulation of photosynthesis in pre-existing needles under elevated [CO(2)]. Conversely, lower excitation pressure adjusted for the efficiency of PSII photochemistry ((1 - q(P)) F(v)'/F(m)') was observed in plants grown under elevated [CO(2)] around budbreak when compared at their growth [CO(2)] (i.e., comparing (1 - q(P)) F(v)'/F(m)' measured at 720 μmol mol(-1) in elevated-CO(2)-grown plants with that at 360 μmol mol(-1) in ambient-CO(2)-grown plants), which suggests lower rate of photoinactivation of PSII in the elevated-CO(2)-grown plants around spring budbreak. The degree of photoinhibition, as indicated by the overnight-dark-adapted F(v)/F(m), however, showed no difference between CO(2) treatments, thereby suggesting that photoprotection during the daytime or the repair of PSII at night was sufficient to alleviate differences in the rate of photoinactivation. | Carbon Dioxide, Chlorophyll, Down-Regulation, Fluorescence, Light, Meristem, Photosynthesis, Photosystem II Protein Complex, Picea, Plant Leaves, Seasons, Seedlings, Starch, Trees | null |
22,705,865 | 2012-11-30 | 2018-12-01 | 1879-1026 | The Science of the total environment | Wintertime spatio-temporal variation of ultrafine particles in a Belgian city. | Mishra Vinit K, Kumar Prashant, Van Poppel Martine, Bleux Nico, Frijns Evelien, Reggente Matteo, Berghmans Patrick, Int Panis Luc, Samson Roeland | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Air Pollutants, Particulate Matter, Vehicle Emissions | IM | 22705865, S0048-9697(12)00736-X, 10.1016/j.scitotenv.2012.05.054 | Simultaneous measurements of ultrafine particles (UFPs) were carried out at four sampling locations situated within a 1 km(2) grid area in a Belgian city, Borgerhout (Antwerp). All sampling sites had different orientation and height of buildings and dissimilar levels of anthropogenic activities (mainly traffic volume). The aims were to investigate: (i) the spatio-temporal variation of UFP within the area, (ii) the effect of wind direction with respect to the volume of traffic on UFP levels, and (iii) the spatial representativeness of the official monitoring station situated in the study area. All sampling sites followed similar diurnal patterns of UFP variation, but effects of local traffic emissions were evident. Wind direction also had a profound influence on UFP concentrations at certain sites. The results indicated a clear influence of local weather conditions and the more dominant effect of traffic volumes. Our analysis indicated that the regional air quality monitoring station represented the other sampling sites in the study area reasonably well; temporal patterns were found to be comparable though the absolute average concentrations showed differences of up to 35%. | Air Pollutants, Belgium, Cities, Environmental Monitoring, Particle Size, Particulate Matter, Seasons, Vehicle Emissions, Weather, Wind | null |
22,705,866 | 2012-11-30 | 2024-11-20 | 1879-1026 | The Science of the total environment | Comparison of fecal indicators with pathogenic bacteria and rotavirus in groundwater. | Ferguson Andrew S, Layton Alice C, Mailloux Brian J, Culligan Patricia J, Williams Daniel E, Smartt Abby E, Sayler Gary S, Feighery John, McKay Larry D, Knappett Peter S K, Alexandrova Ekaterina, Arbit Talia, Emch Michael, Escamilla Veronica, Ahmed Kazi Matin, Alam Md Jahangir, Streatfield P Kim, Yunus Mohammad, van Geen Alexander | eng | R01 TW008066 (FIC NIH HHS, United States); R24 HD050924 (NICHD NIH HHS, United States); 5R01TW8066-2 (FIC NIH HHS, United States) | Comparative Study, Journal Article, Research Support, N.I.H., Extramural | Drinking Water | IM | 22705866, S0048-9697(12)00742-5, 10.1016/j.scitotenv.2012.05.060, PMC3587152, NIHMS387725, 15098084, 20477877, 15105181, 17159896, 16597991, 20236332, 20038692, 21075875, 10523534, 12446905, 19402401, 20639358, 18422953, 12839768, 19270143, 10831460, 12147489, 21226536, 19712380, 17135437, 3028333, 22048430, 20822128, 20497484, 18839059, 15933017, 16751534, 17036837, 17984808, 12571044, 1830197, 20622124, 15296305, 17370511, 14871633, 18202442, 19931715, 17170279, 2231712, 16082943, 12764516, 17496946, 18291437 | Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict total bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary. | Bacteroides, Bangladesh, Coliphages, Drinking Water, Enterobacteriaceae, Escherichia coli, Feces, Groundwater, Humans, Rotavirus, Shigella, Vibrio, Water Microbiology | null |
22,705,867 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Vertical profiles of black carbon aerosols over the urban locations in South India. | Safai P D, Raju M P, Maheshkumar R S, Kulkarni J R, Rao P S P, Devara P C S | eng | null | Journal Article | Aerosols, Air Pollutants, Carbon | IM | 22705867, S0048-9697(12)00740-1, 10.1016/j.scitotenv.2012.05.058 | Vertical profiles of black carbon (BC) aerosol were determined from aircraft measurements under the Cloud Aerosol Interaction and Precipitation Enhancement Experiment (CAIPEEX) program conducted by the Indian Institute of Tropical Meteorology, India during 2009 over Bangalore and Hyderabad in south India. BC mass loadings decreased approximately monotonically from 10(3) to 10(4) ng/m(3) at the surface to ~10(2) ng/m(3) at an altitude of about 7 km; although layers at intermediate levels containing anomalously high BC loadings were frequently encountered that were attributed mainly to the convective transport from surface sources accompanied by changes in the local boundary layer and atmospheric stability. In addition, as evidenced from air mass back trajectories; long range transport from distant sources contributed to some anomalous spikes in BC concentration. The presence of BC in cloud forming regions of the free troposphere could have important implications for cloud microphysics and subsequent rainfall mechanism over this region. Apart from this, the effects on human health are equally important. | Aerosols, Air Pollutants, Altitude, Carbon, India | null |
22,705,871 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Effect of storm events on riverine nitrogen dynamics in a subtropical watershed, southeastern China. | Chen Nengwang, Wu Jiezhong, Hong Huasheng | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | null | 22705871, S0048-9697(12)00754-1, 10.1016/j.scitotenv.2012.05.072 | Rain storms are predicted to increase in the subtropical region due to climate change. However, the effects of storm events on riverine nitrogen (N) dynamics are poorly understood. In this study, the riverine N dynamics and storm effects in a large subtropical river (North Jiulong River, southeastern China) were investigated through continuous sampling of two storm events which occurred in June 2010 and June 2011. The results disclosed a strong linkage between N dynamics and hydrological controls and watershed characteristics. The extreme storm in June 2010 resulted in more fluctuations in N concentrations, loads, and composition, compared with the moderate storm in June 2011. There were contrasting patterns (e.g., the hysteresis effect) between nitrate and ammonium behavior in storm runoff, reflecting their different supply source and transport mechanism. Overall, nitrate supply originated from subsurface runoff and was dominated by within-channel mobilization, while ammonium was mainly from over-land sources and flushed by surface runoff. Extreme storm runoff (2010) caused a four-fold increase in dissolved inorganic N fluxes (DIN), with a greater fraction of ammonium (up to 30% of DIN) compared with the moderate storm and background flow condition (less than 15%). Storm-driven sharp increases of N loads and changes in nutrient stoichiometry (more ammonium) might have been connected with algal blooms in the adjacent estuary and Xiamen Bay. Combined with the background flow measurement of N gradients along the main river and a stream together with anthropogenic N load information, the interactive effect of hydrological and biogeochemical process on riverine N was preliminarily revealed. Current results suggested that storm runoff N was controlled by rainfall, hydrological condition, antecedent soil moisture, spatial variability of land-based N source, and damming. These findings could be used as a reference for future water quality monitoring programs and the development of a pollution mitigation strategy. | null | null |
22,705,872 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Simulation and prediction of phenolic compounds fate in Songhua River, China. | Wang Ce, Feng Yujie, Gao Peng, Ren Nanqi, Li Bai-Lian | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | null | 22705872, S0048-9697(12)00729-2, 10.1016/j.scitotenv.2012.05.047 | The demand for numerical models to simulate and predict the transport and fate of organic pollutants in the environment is a reflection of certain restrictions of experimental research. Various phenolic pollutants have been detected at different concentration levels in the Songhua River, China. A multimedia fate model is coupled with the kinematic wave transport equations to accurately and simply characterize dynamic water flow, velocity and depth. The resulting model is applied to describe the temporal and spatial behavior of eight phenol compounds in the Songhua River. The predictive power of this model is evaluated by statistical tests using field observations during different hydrological seasons of the year 2007. The goodness-of-fit of model prediction for phenolic contaminants is, in general, agreeable. For one focal compound, the pathways and mass fluxes from the river system to the surrounding environment are also evaluated. Uncertainty analysis is performed by Monte Carlo stimulation to judge the influence of variability of input parameters on modeled results. The results indicate that the model has the ability to provide decision makers with valuable reference data for consideration of water pollution controls. Model simulation indicates that phenolic pollution in the river during the low-flow period of 2009 is remarkably reduced when compared to the same period of 2007. | null | null |
22,705,868 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Climatic warming and overgrazing induced the high concentration of organic matter in Lake Hulun, a large shallow eutrophic steppe lake in northern China. | Chen Xiaofeng, Chuai Xiaoming, Yang Liuyan, Zhao Huiying | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | null | 22705868, S0048-9697(12)00734-6, 10.1016/j.scitotenv.2012.05.052 | An abnormally high concentration of organic matter (OM) in Lake Hulun, a large shallow eutrophic lake located in the sparsely populated Hulun Buir Steppe, was observed in a field investigation. Little was known about the origin of the OM. To identify the source of the OM in Lake Hulun, the carbon/nitrogen (C/N) ratio, natural abundance of stable isotope and three dimensional excitation emission matrix (3DEEM) fluorescence spectroscopy techniques were employed. Furthermore, a cyanobacterial incubation and degradation experiment was conducted in the laboratory to quantify the contribution of algae to dissolved organic matter (DOM) in Lake Hulun. C/N, the stable carbon isotope (δ(13)C) values typical of C3 plant debris in particulate organic matter (POM) and the fluorescence indices of DOM indicate that most of the OM in Lake Hulun is of terrigenous origin. It was deduced that only about 10.2% and 7.3% of DOM were contributed by algae in September and January, respectively, according to the linear correlation between the concentrations of algae-derived DOM and the fluorescence intensities of tyrosine-like matter. According to the stockbreeding development and climate change in Hunlun Buir Steppe, we deduced that the destruction of the grassland ecosystem by overgrazing in specific locations and trends in climatic warming and drying were the main factors causing the increase of OM and nutrient concentrations in Lake Hulun. This result highlights the need to pay more attention to the inputs of terrigenous organic matter to the lakes in northern China. | null | null |
22,705,869 | 2012-11-30 | 2018-12-01 | 1879-1026 | The Science of the total environment | Natural and anthropogenic lead in soils and vegetables around Guiyang city, southwest China: a Pb isotopic approach. | Li Fei-Li, Liu Cong-Qiang, Yang Yuan-Gen, Bi Xiang-Yang, Liu Tao-Ze, Zhao Zhi-Qi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Isotopes, Soil Pollutants, Lead | IM | 22705869, S0048-9697(12)00722-X, 10.1016/j.scitotenv.2012.05.040 | Soils, vegetables and rainwaters from three vegetable production bases in the Guiyang area, southwest China, were analyzed for Pb concentrations and isotope compositions to trace its sources in the vegetables and soils. Lead isotopic compositions were not distinguishable between yellow soils and calcareous soils, but distinguishable among sampling sites. The highest (207)Pb/(206)Pb and (208)Pb/(206)Pb ratios were found for rainwaters (0.8547-0.8593 and 2.098-2.109, respectively), and the lowest for soils (0.7173-0.8246 and 1.766-2.048, respectively). The (207)Pb/(206)Pb and (208)Pb/(206)Pb ratios increased in vegetables in the order of roots<stems<leaves<fruits. Plots of the (207)Pb/(206)Pb ratios versus the (208)Pb/(206)Pb ratios from all samples formed a straight line and supported a binary end-member mixing model for Pb in vegetables. Using deep soils and rainwaters as geogenic and anthropogenic end members in the mixing model, it was estimated that atmospheric Pb contributed 30-77% to total Pb for vegetable roots, 43-71% for stems, 72-85% for leaves, and 90% for capsicum fruits, whereas 10-70% of Pb in all vegetable parts was derived from soils. This research supports that heavy metal contamination in vegetables can result mainly from atmospheric deposition, and Pb isotope technique is useful for tracing the sources of Pb contamination in vegetables. | China, Cities, Environmental Monitoring, Isotopes, Lead, Plant Leaves, Plant Roots, Plant Stems, Rain, Soil Pollutants, Vegetables | null |
22,705,870 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Cumulative impacts of urban runoff and municipal wastewater effluents on wild freshwater mussels (Lasmigona costata). | Gillis Patricia L | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Metals, Heavy, Water Pollutants, Chemical | IM | 22705870, S0048-9697(12)00743-7, 10.1016/j.scitotenv.2012.05.061 | Aquatic biota living in urban watersheds, are chronically exposed to a complex mixture of contaminants from various sources, including municipal wastewater effluents and road runoff. This study examined the general condition, immune function and contaminant load of wild freshwater mussels collected from a large urbanized river over three consecutive field seasons. Four study sites along the Grand River (ON) were selected to represent an incremental contaminant exposure, such that mussels collected from the final downstream site were exposed to the cumulative inputs from 11 municipal wastewater treatment plants and road runoff from four cities. Wild mussels collected downstream of the urban area had significantly lower (p<0.05) condition factor and did not live as long (significantly reduced mean age) as the mussels collected upstream of the cities. There appears to be a trend of increasing proportions of gravid females at the downstream sites, but whether this trend indicates feminization of the mussel population or is simply an artifact of sampling effort is unclear. An examination of hemocyte phagocytosis revealed a pattern of increasing immune activity at the downstream sites, but only in one of the years sampled. The significant and cumulative increase in Cu, Pb, Zn, Al, Cr, and Ni in the gills of downstream mussels indicates that metals are bioavailable in this ecosystem and that tissue concentrations increase with multiple urban inputs. While the complex nature of the exposure prevents identification of the cause(s) of the observed effects, some contaminants such as ammonia and chloride reach levels known to be toxic to freshwater mussels at the downstream sites. These results indicate that chronic exposure to multiple contaminants negatively impacts mussel health and longevity and corroborates previous assumptions that waterborne contaminants contributed to the decline of the freshwater mussel populations in this watershed. | Age Factors, Animals, Environmental Exposure, Female, Fresh Water, Hemocytes, Male, Metals, Heavy, Ontario, Reproduction, Rivers, Unionidae, Waste Disposal, Fluid, Water Pollutants, Chemical | null |
22,705,873 | 2012-11-30 | 2012-07-03 | 1879-1026 | The Science of the total environment | Ecotoxicological risk assessment linked to infilling quarries with treated dredged seaport sediments. | Perrodin Yves, Donguy Gilles, Bazin Christine, Volatier Laurence, Durrieu Claude, Bony Sylvie, Devaux Alain, Abdelghafour Mohammed, Moretto Robert | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Water Pollutants, Chemical | IM | 22705873, S0048-9697(12)00751-6, 10.1016/j.scitotenv.2012.05.069 | The dredged sediments of polluted seaports now raise complex management problems since it is no longer possible to discharge them into the sea. This results in the need to manage them on land, raising other types of technical, economic and environmental problems. Regarding the technical and economic dimensions, traditional waste treatment methods have proved to be poorly adapted, due to very high costs and low absorbable volumes. In this context, filling quarries in coastal areas with treated sediments could represent an interesting alternative for these materials. Nevertheless, for the environmental dimension, it is necessary to demonstrate that this possibility is harmless to inland ecosystems. Consequently, a specific ecotoxicological risk assessment methodology has been formulated and tested on three sediments taken from seaboards of France, in view to providing an operational and usable tool for the prior validation of any operation to fill quarries with treated seaport sediments. This method incorporates the formulation of a global conceptual model of the scenario studied and the definition of protocols for each of its steps: the characterisation of exposures (based on a simulation of sediment deposit), the characterisation of effects (via the study of sediments ecotoxicity), and the final ecotoxicological risk assessment performed as a calculation of a risk quotient. It includes the implementation in parallel of two types of complementary approach: the "substances" approach derived from the European methodology for assessing new substances placed on the market, and the "matrix" approach which is similar to methods developed in France to assess ecological risks in other domains (waste management, polluted site management, …). The application of this dual approach to the three sediments tested led to conclude with reliability that the project to deposit sediments "1" and "2" presented a low risk for the peripheral aquatic ecosystems while sediment "3" presented a high risk. | Animals, Aquatic Organisms, Ecotoxicology, Environmental Exposure, Environmental Pollution, France, Geologic Sediments, Risk Assessment, Waste Management, Water Pollutants, Chemical | null |
22,705,874 | 2012-11-30 | 2022-03-21 | 1879-1026 | The Science of the total environment | Modeling the transmission dynamics of dengue fever: implications of temperature effects. | Chen Szu-Chieh, Hsieh Meng-Huan | eng | null | Journal Article | null | IM | 22705874, S0048-9697(12)00658-4, 10.1016/j.scitotenv.2012.05.012 | The purpose of this study was to investigate the transmission dynamic modeling of dengue fever in subtropical Taiwan by the contributing temperature-dependent entomological parameters of Aedes aegypti. A vector-host transmission model was used to explore the temperature variation of pre-adult mosquito maturation, oviposition rate, adult mosquito death rate, and virus incubation rate in the mosquito. Sensitivity analysis, by transmissible biting rate and the initial mosquito population, was adapted to observe features of the epidemic. This study showed that the entomological parameter estimates are positively correlated with a gradual temperature increase, but not with pre-adult mosquito maturation rate and mosquito death rate, indicating the limitation of pre-adult mosquito maturation rate. Results show that the temperature climate factor was indeed important and influenced the dynamic modeling of the vector-host interaction. Results from our simulation also suggest that the greatest risk of dengue transmission occurs at temperatures equal to 28 °C. In the future, these results could be used for control measures modeling and cost-effectiveness analysis. | Aedes, Animals, Dengue, Female, Humans, Insect Vectors, Models, Biological, Mortality, Oviposition, Taiwan, Temperature | null |
22,705,876 | 2013-07-26 | 2022-03-11 | 1759-8486 | Journal of neurointerventional surgery | Standard of practice: endovascular treatment of intracranial atherosclerosis. | Hussain M Shazam, Fraser Justin F, Abruzzo Todd, Blackham Kristine A, Bulsara Ketan R, Derdeyn Colin P, Gandhi Chirag D, Hirsch Joshua A, Hsu Daniel P, Jayaraman Mahesh V, Meyers Philip M, Narayanan Sandra, Prestigiacomo Charles J, Rasmussen Peter A | eng | null | Journal Article, Review | null | IM | 22705876, neurintsurg-2012-010405, 10.1136/neurintsurg-2012-010405 | Symptomatic intracranial atherosclerotic disease (ICAD) worldwide represents one of the most prevalent causes of stroke. When severe, studies show that it has a very high risk for recurrent stroke, highlighting the need for effective preventative strategies. The mainstay of treatment has been medical therapy and is of critical importance in all patients with this disease. Endovascular therapy is also a possible therapeutic option but much remains to be defined in terms of best techniques and patient selection. This guideline will serve as recommendations for diagnosis and endovascular treatment of patients with ICAD. | Animals, Endovascular Procedures, Humans, Intracranial Arteriosclerosis, Standard of Care, Treatment Outcome | null |
22,705,878 | 2012-12-14 | 2018-12-01 | 1873-3476 | International journal of pharmaceutics | The structure and function of the stratum corneum. | Menon Gopinathan K, Cleary Gary W, Lane Majella E | eng | null | Journal Article, Review | null | IM | 22705878, S0378-5173(12)00602-3, 10.1016/j.ijpharm.2012.06.005 | Over the past 150 years the skin's structure and function has been the subject of much investigation by scientists. The stratum corneum (SC), the skin's outermost layer and interface with the outside world is now well recognized as the barrier that prevents unwanted materials from entering, and excessive loss of water from exiting the body. This review summarizes the major advances in our understanding of this formidable membrane. The structure of the SC is outlined as well as techniques to visualize the barrier. The lipid organization and ionic gradients, as well as the metabolic responses and underlying cellular signalling that lead to barrier repair and homeostasis are discussed. Finally, a brief overview of the molecular and genetic factors that determine the development of a competent permeability barrier is provided. | Animals, Epidermal Cells, Epidermis, Gene Expression Regulation, Humans, Wound Healing | null |
22,705,877 | 2013-01-04 | 2021-01-12 | 1872-8057 | Molecular and cellular endocrinology | Somatostatin analogs and chimeric somatostatin-dopamine molecules differentially regulate human growth hormone and prolactin gene expression and secretion in vitro. | Gruszka Anna, Culler Michael D, Melmed Shlomo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | BIM 23120, BIM 23206, BIM-23A761, Receptors, Somatostatin, Human Growth Hormone, Somatostatin, Prolactin, TBR-760, Dopamine | IM | 22705877, S0303-7207(12)00314-0, 10.1016/j.mce.2012.05.020 | We tested effects of selective somatostatin receptor 2 (SST2) agonist BIM-23120, SST5 agonist BIM-23206 and chimeric somatostatin-dopamine molecules (SRIF/DA) BIM-23A760 and BIM-23A761 on GH and PRL secretion and gene expression in human GH/PRL-secreting pituitary tumors in vitro. In "responders" group BIM-23120 suppressed GH levels by 26±4%, BIM-23206 by 31±5%, BIM-23A760 by 23±4%, BIM-23A761 by 39±8% and D(2)-dopamine agonist BIM-53097 by 31±5%. Using real-time PCR we demonstrated that GH inhibition was not accompanied by decreased GH mRNA levels. PRL secretion was inhibited by BIM-23A760 (29±5%), BIM-23A761 (34±4%), BIM-23206 (26±4%) and BIM-53097 (36±2%). SRIF/DA and BIM-53097 also suppressed PRL mRNA levels. Concluding, SST2 and SST5 agonists and SRIF/DA inhibit GH secretion, but do not suppress GH gene transcription. SRIF/DA and BIM-53097 inhibit both PRL secretion and PRL gene expression. SST5 agonist inhibits PRL secretion, but does not suppress PRL gene expression. D(2) affinity is crucial in SRIF/DA action on PRL gene expression. | Adenoma, Adult, Aged, Dopamine, Female, Gene Expression Regulation, Neoplastic, Human Growth Hormone, Humans, Male, Middle Aged, Pituitary Neoplasms, Prolactin, Receptors, Somatostatin, Somatostatin, Transcription, Genetic, Tumor Cells, Cultured | null |
22,705,875 | 2014-02-19 | 2013-06-13 | 1759-8486 | Journal of neurointerventional surgery | Safety of full-dose intravenous recombinant tissue plasminogen activator followed by multimodal endovascular therapy for acute ischemic stroke. | Nogueira Raul G, Yoo Albert J, Masrur Shihab, Batista Leonardo M, Hakimelahi Reza, Hirsch Joshua A, Schwamm Lee H | eng | null | Journal Article | Recombinant Proteins, Tissue Plasminogen Activator | IM | 22705875, neurintsurg-2012-010376, 10.1136/neurintsurg-2012-010376 | The optimal management of stroke patients who fail treatment with intravenous recombinant tissue plasminogen activator (rt-PA) remains unknown. A study was undertaken to establish whether treatment with a standard intravenous t-PA dose (0.9 mg/kg) followed by multimodal endovascular therapy would have a similar safety profile to reduced dose (0.6 mg/kg) bridging therapy. | Adolescent, Adult, Aged, Aged, 80 and over, Brain Ischemia, Combined Modality Therapy, Endovascular Procedures, Female, Humans, Injections, Intravenous, Male, Middle Aged, Prospective Studies, Recombinant Proteins, Retrospective Studies, Stroke, Thrombolytic Therapy, Tissue Plasminogen Activator, Treatment Outcome, Young Adult | Stroke, aneurysm, angiography, angioplasty, arteriovenous malformation, artery, atherosclerosis, balloon, brain, catheter, coil, complication, drug, embolic, intravenous thrombolysis, malformation, rt-PA, stenosis, stent, stroke, subarachnoid, technique, technology, thrombectomy, thrombolysis, tumor |
22,705,879 | 2012-12-10 | 2013-11-21 | 1096-0937 | Fungal genetics and biology : FG & B | Characterization of PIR1, a GATA family transcription factor involved in iron responses in the white-rot fungus Phanerochaete chrysosporium. | Canessa Paulo, Muñoz-Guzmán Felipe, Vicuña Rafael, Larrondo Luis F | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fungal Proteins, GATA Transcription Factors, Iron, Oxidoreductases | IM | 22705879, S1087-1845(12)00101-6, 10.1016/j.fgb.2012.05.013 | Iron, although toxic in excess, is an essential element for biological systems. Therefore, its homeostasis is of critical importance and tight mechanisms participate in its acquisition by microbial organisms. Lately, the relevance of this metal for biomass conversion by wood-degrading fungi has been gaining increasing attention. Iron plays a critical role as cofactor of key enzymes such as lignin and manganese peroxidases in lignin-degrading white-rot fungi, while Fe(II) also serves a pivotal role in Fenton reactions that are central in cellulose depolymerization by brown-rotters. It has been hypothesized that multicopper oxidases with ferroxidase activity might participate in controlling the bioavailability of iron in the hyphal proximity, fine-tuning Fenton chemistry and balancing lignin versus cellulose degradation. In order to further explore the dynamics of iron regulation in the well known white-rot fungus Phanerochaete chrysosporium, we analyzed the mRNA levels of the multicopper oxidases genes (mcos) in response to iron supplementation, confirming down-regulation of their expression in response to this metal. To gain a better understanding on the transcriptional mechanisms mediating this effect, we searched for a gene encoding a GATA-type transcription factor with homology to URBS1, the major transcriptional regulator of iron homeostasis in Ustilago maydis. Due to the limitation of experimental tools in P. chrysosporium, the alleged Phanerochaete iron regulator (PIR1) was studied by complementation of a Neurospora SRE/URBS1-deficient strain, where phenotypic and molecular characteristics of this transcriptional regulator could be easily assessed. In addition, using a genome-wide in silico strategy, we searched for putative cis-acting iron-responsive elements in P. chrysosporium. Some of the identified genes showed reduced transcript levels after 30 min in the presence of the metal, consistent with an SRE/URBS1-mediated mechanism, and suggesting a broad effect of iron on the regulation of several cellular processes. | Base Sequence, Consensus Sequence, Fungal Proteins, GATA Transcription Factors, Gene Order, Iron, Oxidoreductases, Phanerochaete, Response Elements, Transcription, Genetic | null |
22,705,882 | 2012-11-19 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | DNA and nuclear aggregates of polyamines. | Iacomino Giuseppe, Picariello Gianluca, D'Agostino Luciano | eng | null | Journal Article, Review | Polyamines, DNA | IM | 22705882, S0167-4889(12)00166-8, 10.1016/j.bbamcr.2012.05.033 | Polyamines (PAs) are linear polycations that are involved in many biological functions. Putrescine, spermidine and spermine are highly represented in the nucleus of eukaryotic cells and have been the subject of decades of extensive research. Nevertheless, their capability to modulate the structure and functions of DNA has not been fully elucidated. We found that polyamines self-assemble with phosphate ions in the cell nucleus and generate three forms of compounds referred to as Nuclear Aggregates of Polyamines (NAPs), which interact with genomic DNA. In an in vitro setting that mimics the nuclear environment, the assembly of PAs occurs within well-defined ratios, independent of the presence of the DNA template. Strict structural and functional analogies exist between the in vitro NAPs (ivNAPs) and their cellular homologues. Atomic force microscopy showed that ivNAPs, as theoretically predicted, have a cyclic structure, and in the presence of DNA, they form a tube-like arrangement around the double helix. Features of the interaction between ivNAPs and genomic DNA provide evidence for the decisive role of "natural" NAPs in regulating important aspects of DNA physiology, such as conformation, protection and packaging, thus suggesting a new vision of the functions that PAs accomplish in the cell nucleus. | Animals, Cell Nucleus, Cyclization, DNA, Humans, Molecular Conformation, Nanotubes, Polyamines | null |
22,705,880 | 2012-12-10 | 2016-11-25 | 1096-0937 | Fungal genetics and biology : FG & B | Transcriptome analyses during fruiting body formation in Fusarium graminearum and Fusarium verticillioides reflect species life history and ecology. | Sikhakolli Usha Rani, López-Giráldez Francesc, Li Ning, Common Ralph, Townsend Jeffrey P, Trail Frances | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Peptides, Mating Factor | IM | 22705880, S1087-1845(12)00097-7, 10.1016/j.fgb.2012.05.009 | Fusarium graminearum and F. verticillioides are devastating cereal pathogens with very different life history and ecological characteristics. F. graminearum is homothallic, and sexual spores are an important component of its life cycle, responsible for disease initiation. F. verticilloides is heterothallic, and produces only modest numbers of fruiting bodies, which are not a significant source of inoculum. To identify corresponding differences in the transcriptional program underlying fruiting body development in the two species, comparative expression was performed, analyzing six developmental stages. To accompany the transcriptional analysis, detailed morphological characterization of F. verticillioides development was performed and compared to a previous morphological analysis of F. graminearum. Morphological development was similar between the two species, except for the observation of possible trichogynes in F. verticillioides ascogonia, which have not been previously reported for any Fusarium species. Expression of over 9000 orthologous genes were measured for the two species. Functional assignments of highly expressed orthologous genes at each time-point revealed the majority of highly expressed genes fell into the "unclassified proteins" category, reflecting the lack of characterization of genes for sexual development in both species. Simultaneous examination of morphological development and stage-specific gene expression suggests that degeneration of the paraphyses during sexual development is an apoptotic process. Expression of mating type genes in the two species differed, possibly reflecting the divergent roles they play in sexual development. Overall, the differences in gene expression reflect the greater role of fruiting bodies in the life cycle and ecology of F. graminearum versus F. verticillioides. | Apoptosis, Cluster Analysis, Computational Biology, Ecosystem, Fruiting Bodies, Fungal, Fusarium, Gene Expression Profiling, Gene Expression Regulation, Fungal, Lipid Metabolism, Mating Factor, Peptides, Phenotype, Transcriptome | null |
22,705,883 | 2012-11-19 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | Connexin 43 phosphorylation and degradation are required for adipogenesis. | Yeganeh Azadeh, Stelmack Gerald L, Fandrich Robert R, Halayko Andrew J, Kardami Elissavet, Zahradka Peter | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Connexin 43, Leupeptins, benzyloxycarbonylleucyl-leucyl-leucine aldehyde | IM | 22705883, S0167-4889(12)00165-6, 10.1016/j.bbamcr.2012.06.009 | Connexin-43 (Cx43) is a membrane phosphoprotein that mediates direct inter-cellular communication by forming gap junctions. In this way Cx43 can influence gene expression, differentiation and growth. Its role in adipogenesis, however, is poorly understood. In this study, we established that Cx43 becomes highly phosphorylated in early adipocyte differentiation and translocates to the plasma membrane from the endoplasmic reticulum. As preadipocytes differentiate, Cx43 phosphorylation declines, the protein is displaced from the plasma membrane, and total cellular levels are reduced via proteosomal degradation. Notably, we show that inhibiting Cx43 degradation or constitutively over-expressing Cx43 blocks adipocyte differentiation. These data reveal that transient activation of Cx43 via phosphorylation followed by its degradation is vital for preadipocyte differentiation and maturation of functional adipocytes. | 3T3-L1 Cells, Adipocytes, Adipogenesis, Adipose Tissue, Animals, Connexin 43, Laser Scanning Cytometry, Leupeptins, Mice, Phosphorylation, Protein Transport, Proteolysis, Subcellular Fractions | null |
22,705,884 | 2012-11-19 | 2022-12-07 | 0006-3002 | Biochimica et biophysica acta | Role of mitochondrial uncoupling protein 2 in cancer cell resistance to gemcitabine. | Dalla Pozza Elisa, Fiorini Claudia, Dando Ilaria, Menegazzi Marta, Sgarbossa Anna, Costanzo Chiara, Palmieri Marta, Donadelli Massimo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Ion Channels, Iridoids, Mitochondrial Proteins, RNA, Messenger, UCP2 protein, human, Uncoupling Agents, Uncoupling Protein 2, Deoxycytidine, Superoxides, genipin, Superoxide Dismutase, Poly(ADP-ribose) Polymerases, Acetylcysteine, Gemcitabine | IM | 22705884, S0167-4889(12)00163-2, 10.1016/j.bbamcr.2012.06.007 | Cancer cells exhibit an endogenous constitutive oxidative stress higher than that of normal cells, which renders tumours vulnerable to further reactive oxygen species (ROS) production. Mitochondrial uncoupling protein 2 (UCP2) can mitigate oxidative stress by increasing the influx of protons into the mitochondrial matrix and reducing electron leakage and mitochondrial superoxide generation. Here, we demonstrate that chemical uncouplers or UCP2 over-expression strongly decrease mitochondrial superoxide induction by the anticancer drug gemcitabine (GEM) and protect cancer cells from GEM-induced apoptosis. Moreover, we show that GEM IC(50) values well correlate with the endogenous level of UCP2 mRNA, suggesting a critical role for mitochondrial uncoupling in GEM resistance. Interestingly, GEM treatment stimulates UCP2 mRNA expression suggesting that mitochondrial uncoupling could have a role also in the acquired resistance to GEM. Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing strongly enhances GEM-induced mitochondrial superoxide generation and apoptosis, synergistically inhibiting cancer cell proliferation. These events are significantly reduced by the addition of the radical scavenger N-acetyl-l-cysteine or MnSOD over-expression, demonstrating a critical role of the oxidative stress. Normal primary fibroblasts are much less sensitive to GEM/genipin combination. Our results demonstrate for the first time that UCP2 has a role in cancer cell resistance to GEM supporting the development of an anti-cancer therapy based on UCP2 inhibition associated to GEM treatment. | Acetylcysteine, Apoptosis, Cell Line, Tumor, Cell Proliferation, Deoxycytidine, Drug Resistance, Neoplasm, Gene Expression Regulation, Neoplastic, Humans, Ion Channels, Iridoids, Mitochondrial Proteins, Neoplasms, Poly(ADP-ribose) Polymerases, RNA, Messenger, Superoxide Dismutase, Superoxides, Uncoupling Agents, Uncoupling Protein 2, Gemcitabine | null |
22,705,881 | 2012-12-13 | 2018-12-01 | 1092-874X | International journal of toxicology | Dried blood spot analysis of donepezil in support of a GLP 3-month dose-range finding study in rats. | Meier-Davis Susan R, Meng Min, Yuan Weiwei, Diehl Lisa, Arjmand Fatima M, Lucke Rebecca M, Huang Betsy, Wen Jianye, Shudo Jutaro, Nagata Tetsuto | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Cholinesterase Inhibitors, Indans, Piperidines, Donepezil | IM | 22705881, 1091581812447957, 10.1177/1091581812447957 | Donepezil hydrochloride is a reversible acetyl cholinesterase inhibitor approved for Alzheimer disease treatment. As an alternate therapy, a donepezil hydrochloride transdermal patch is in development. Recommended nonclinical safety studies include a 3-month Good Laboratory Practice (GLP) dose-range finding (DRF) study prior to conducting the 2-year dermal carcinogenicity study in rats. Demonstration of systemic exposure is necessary to interpret the in vivo data. Previous nonclinical reports supporting oral dosing have utilized liquid chromatography tandem mass spectrometry (LC/MS/MS) to quantify donepezil concentrations in plasma. Smaller species with limited blood volumes do not allow serial sampling to derive the full pharmacokinetic profile from a single animal. Therefore, the option of another analytical method requiring decreased sample volumes is desirable as it would decrease the required number of animals while obtaining the complete profile. The dried blood spot (DBS) technique allows drug level measurement from a few microliters; however, the method is still not widely utilized in GLP studies. Because donepezil plasma levels are known by the oral route, DBS was used to bridge the previous oral data and to support a 13-week GLP DRF study for repeated topical application in rats, comparing oral administration with 4 topical formulations. The DBS method was validated and demonstrated robustness and reproducibility for application to the DRF study. The assay results were comparable to a previously reported plasma LC/MS/MS assay-derived pharmacokinetic profile and provided justification for selection of the topical formulation and dose levels for the subsequent dermal carcinogenicity study. | Administration, Oral, Administration, Topical, Animals, Cholinesterase Inhibitors, Chromatography, Liquid, Donepezil, Dose-Response Relationship, Drug, Dried Blood Spot Testing, Female, Indans, Laboratories, Male, Piperidines, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Tandem Mass Spectrometry, Toxicity Tests | null |
22,705,888 | 2012-09-17 | 2025-01-03 | 1524-4539 | Circulation | Preventing platelet thrombosis with a PAR1 pepducin. | Abdel-Latif Ahmed, Smyth Susan S | eng | UL1 TR000117 (NCATS NIH HHS, United States); UL1RR033173 (NCRR NIH HHS, United States); UL1 RR033173 (NCRR NIH HHS, United States); R01 HL078663 (NHLBI NIH HHS, United States); UL1 TR001998 (NCATS NIH HHS, United States); R01HL078663 (NHLBI NIH HHS, United States); P20 GM103527 (NIGMS NIH HHS, United States) | Editorial, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | F2pal-16 pepducin, Lipopeptides, Platelet Aggregation Inhibitors, Receptor, PAR-1 | IM | 22705888, CIRCULATIONAHA.112.122317, 10.1161/CIRCULATIONAHA.112.122317, PMC3926097, NIHMS550015, 20798382, 20124733, 12970118, 8918275, 11786451, 19286091, 22077816, 22705889, 21502577, 12070020, 20805115, 21160056, 22443427 | null | Animals, Blood Platelets, Humans, Lipopeptides, Platelet Aggregation Inhibitors, Receptor, PAR-1, Signal Transduction, Thrombosis | null |
22,705,886 | 2012-12-03 | 2021-10-21 | 1524-4539 | Circulation | Interleukin-10 treatment attenuates pressure overload-induced hypertrophic remodeling and improves heart function via signal transducers and activators of transcription 3-dependent inhibition of nuclear factor-κB. | Verma Suresh Kumar, Krishnamurthy Prasanna, Barefield David, Singh Neha, Gupta Rajesh, Lambers Erin, Thal Melissa, Mackie Alexander, Hoxha Eneda, Ramirez Veronica, Qin Gangjian, Sadayappan Sakthivel, Ghosh Asish K, Kishore Raj | eng | HL091983 (NHLBI NIH HHS, United States); R01 HL095874 (NHLBI NIH HHS, United States); R01 HL053354 (NHLBI NIH HHS, United States); HL105597 (NHLBI NIH HHS, United States); HL053354 (NHLBI NIH HHS, United States); HL107093 (NHLBI NIH HHS, United States); R01 HL091983 (NHLBI NIH HHS, United States); F32 HL107093 (NHLBI NIH HHS, United States); HL095874 (NHLBI NIH HHS, United States); P01 HL108795 (NHLBI NIH HHS, United States); HL108795 (NHLBI NIH HHS, United States); R01 HL105597 (NHLBI NIH HHS, United States); R37 HL053354 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | NF-kappa B, STAT3 Transcription Factor, Stat3 protein, mouse, Interleukin-10, Isoproterenol | IM | 22705886, CIRCULATIONAHA.112.112185, 10.1161/CIRCULATIONAHA.112.112185, PMC3422741, NIHMS394543, 20965166, 22025605, 15965500, 12912791, 15533863, 10986582, 12057007, 10528039, 11692158, 10946312, 16762434, 19780028, 19181934, 16037568, 21537080, 18701451, 20194499, 12456484, 11245652, 19834007, 21527742, 20219984, 21267510, 18926830, 12433833, 17353445, 15870116, 20447565, 16842199, 19096025, 16935932 | Inflammation plays a critical role in adverse cardiac remodeling and heart failure. Therefore, approaches geared toward inhibiting inflammation may provide therapeutic benefits. We tested the hypotheses that genetic deletion of interleukin-10 (IL-10), a potent antiinflammatory cytokine, exacerbates pressure overload-induced adverse cardiac remodeling and hypertrophy and that IL-10 therapy inhibits this pathology. | Animals, Cardiomegaly, Disease Models, Animal, Disease Susceptibility, Fibrosis, Interleukin-10, Isoproterenol, Mice, Mice, Inbred C57BL, Mice, Knockout, Myoblasts, Cardiac, Myocardium, NF-kappa B, Rats, Rats, Sprague-Dawley, STAT3 Transcription Factor, Signal Transduction, Ventricular Dysfunction, Left, Ventricular Remodeling | null |
22,705,885 | 2012-11-19 | 2020-12-09 | 0006-3002 | Biochimica et biophysica acta | The endocytic adaptor protein Disabled-2 is required for cellular uptake of fibrinogen. | Hung Wei-Shan, Huang Chien-Ling, Fan Jui-Ting, Huang Ding-Yuan, Yeh Chun-Fung, Cheng Ju-Chien, Tseng Ching-Ping | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Adaptor Protein Complex 2, Adaptor Proteins, Signal Transducing, Apoptosis Regulatory Proteins, Clathrin, DAB2 protein, human, Platelet Glycoprotein GPIIb-IIIa Complex, RNA, Small Interfering, Tumor Suppressor Proteins, Fibrinogen, Tetradecanoylphorbol Acetate | IM | 22705885, S0167-4889(12)00164-4, 10.1016/j.bbamcr.2012.06.008 | Endocytosis is pivotal for uptake of fibrinogen from plasma into megakaryocytes and platelet α-granules. Due to the complex adaptor and cargo contents in endocytic vehicles, the underlying mechanism of fibrinogen uptake is not yet completely elucidated. In this study, we investigated whether the endocytic adaptor protein Disabled-2 (DAB2) mediates fibrinogen uptake in an adaptor-specific manner. By employing primary megakaryocytes and megakaryocytic differentiating human leukemic K562 cells as the study models, we found that fibrinogen uptake is associated with the expression of integrin αIIbβ3 and DAB2 and is mediated through clathrin-dependent manner. Accordingly, constitutive and inducible knockdown of DAB2 by small interfering RNA reduced fibrinogen uptake for 53.2 ± 9.8% and 59.0 ± 10.7%, respectively. Culturing the cells in hypertonic solution or in the presence of clathrin inhibitor chlorpromazine abrogated clathrin-dependent endocytosis and diminished the uptake of fibrinogen. Consistent with these findings, 72.2 ± 0.2% of cellular DAB2 was colocalized with clathrin, whereas 56.4±4.1% and 54.6 ± 2.0% of the internalized fibrinogen were colocalized with clathrin and DAB2, respectively. To delineate whether DAB2 mediates fibrinogen uptake in an adaptor-specific manner, K562 stable cell lines with knockdown of the adaptor protein-2 (AP-2) or double knockdown of AP-2/DAB2 were established. The AP-2 knockdown cells elicited normal fibrinogen uptake activity but the uptake of collagen was diminished. In addition, collagen uptake was further reduced in DAB2/AP-2 knockdown cells. These findings thereby define an adaptor-specific mechanism in the control of fibrinogen uptake and implicate that DAB2 is the key adaptor in the clathrin-associated endocytic complexes to mediate fibrinogen internalization. | Adaptor Protein Complex 2, Adaptor Proteins, Signal Transducing, Animals, Apoptosis Regulatory Proteins, Cattle, Cell Differentiation, Clathrin, Endocytosis, Fibrinogen, Humans, K562 Cells, Megakaryocytes, Mice, Mice, Inbred C57BL, Models, Biological, Platelet Glycoprotein GPIIb-IIIa Complex, Protein Transport, RNA, Small Interfering, Staining and Labeling, Tetradecanoylphorbol Acetate, Tumor Suppressor Proteins | null |
22,705,889 | 2012-09-17 | 2025-01-03 | 1524-4539 | Circulation | Suppression of arterial thrombosis without affecting hemostatic parameters with a cell-penetrating PAR1 pepducin. | Zhang Ping, Gruber András, Kasuda Shogo, Kimmelstiel Carey, O'Callaghan Katie, Cox Daniel H, Bohm Andrew, Baleja James D, Covic Lidija, Kuliopulos Athan | eng | P50 HL110789 (NHLBI NIH HHS, United States); R01 HL064701 (NHLBI NIH HHS, United States); RC2 HL101783 (NHLBI NIH HHS, United States); R01 HL64701 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | F2pal-16 pepducin, Lipopeptides, Platelet Aggregation Inhibitors, Receptor, PAR-1 | IM | 22705889, CIRCULATIONAHA.112.091918, 10.1161/CIRCULATIONAHA.112.091918, PMC3423084, NIHMS390467, 12538843, 19379698, 21364148, 21781274, 12871297, 17982182, 16505172, 11805322, 17166251, 21536878, 12357249, 14607253, 11005807, 19023091, 19717846, 20124733, 2934045, 21261806, 21502577, 13678420, 21156928, 19249409, 17973080, 12008957, 22077849, 21703428, 22032704, 19098390, 10820018, 19286091, 19652875, 21615752, 21438116, 21935705, 22077816, 12892834, 16952995, 20805115, 22443427, 15182185, 19232896, 18818650, 20124137 | Thrombin-dependent platelet activation is heightened in the setting of percutaneous coronary intervention and may cause arterial thrombosis with consequent myocardial necrosis. Given the high incidence of adverse effects in patients with acute coronary syndromes, there remains an unmet need for the development of new therapeutics that target platelet activation without unduly affecting hemostasis. The thrombin receptor, PAR1, has recently emerged as a promising new target for therapeutic intervention in patients with acute coronary syndromes. | Animals, Cell Membrane, Guinea Pigs, Hemostasis, Humans, Lipopeptides, Macaca fascicularis, Male, Papio anubis, Platelet Activation, Platelet Aggregation Inhibitors, Receptor, PAR-1, Thrombosis | null |
22,705,887 | 2012-12-03 | 2021-10-21 | 1524-4539 | Circulation | Nucleotide excision DNA repair is associated with age-related vascular dysfunction. | Durik Matej, Kavousi Maryam, van der Pluijm Ingrid, Isaacs Aaron, Cheng Caroline, Verdonk Koen, Loot Annemarieke E, Oeseburg Hisko, Bhaggoe Usha Musterd, Leijten Frank, van Veghel Richard, de Vries René, Rudez Goran, Brandt Renata, Ridwan Yanto R, van Deel Elza D, de Boer Martine, Tempel Dennie, Fleming Ingrid, Mitchell Gary F, Verwoert Germaine C, Tarasov Kirill V, Uitterlinden Andre G, Hofman Albert, Duckers Henricus J, van Duijn Cornelia M, Oostra Ben A, Witteman Jacqueline C M, Duncker Dirk J, Danser A H Jan, Hoeijmakers Jan H, Roks Anton J M | eng | R01 HL071039 (NHLBI NIH HHS, United States); N01 AG012100 (NIA NIH HHS, United States); N01 AG062101 (NIA NIH HHS, United States); R01 HL107385 (NHLBI NIH HHS, United States); R01 HL076784 (NHLBI NIH HHS, United States); R01 AG032098 (NIA NIH HHS, United States); P30 DK072488 (NIDDK NIH HHS, United States); N01 AG062103 (NIA NIH HHS, United States); 233424 (European Research Council, International); P01 AG017242 (NIA NIH HHS, United States); 1P01 AG-17242-02 (NIA NIH HHS, United States); R01 AG028321 (NIA NIH HHS, United States); R01 HL070100 (NHLBI NIH HHS, United States); R01 AG018728 (NIA NIH HHS, United States); U01 ES011044 (NIEHS NIH HHS, United States); K12 RR023250 (NCRR NIH HHS, United States); R01 HL060040 (NHLBI NIH HHS, United States); 1UO1 ES011044 (NIEHS NIH HHS, United States); N01 AG062106 (NIA NIH HHS, United States); K24 HL004334 (NHLBI NIH HHS, United States); U01 HL084756 (NHLBI NIH HHS, United States); R01 HL077447 (NHLBI NIH HHS, United States); N01 AG012109 (NIA NIH HHS, United States); U01 HL072515 (NHLBI NIH HHS, United States); R01 HL080124 (NHLBI NIH HHS, United States); R01 HL094898 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | DDB2 protein, human, DNA-Binding Proteins, Endonucleases, Ercc1 protein, mouse, Xeroderma Pigmentosum Group D Protein, Ercc2 protein, mouse | IM | 22705887, CIRCULATIONAHA.112.104380, 10.1161/CIRCULATIONAHA.112.104380, PMC3430727, NIHMS394549, 12496039, 7834842, 16824498, 17183314, 7581825, 16940223, 11327625, 12515756, 20173736, 19934023, 16009385, 18723444, 14726016, 2558661, 20956613, 20031549, 15820197, 11950998, 12186795, 19042039, 11679409, 21276184, 14644313, 20458013, 9052889, 7690550, 22068335, 19812404, 22548406, 19696142, 22953029, 7895356, 22083160, 19767531, 10642303, 8770860, 11927518, 8034885, 17204661 | Vascular dysfunction in atherosclerosis and diabetes mellitus, as observed in the aging population of developed societies, is associated with vascular DNA damage and cell senescence. We hypothesized that cumulative DNA damage during aging contributes to vascular dysfunction. | Aging, Animals, Blood Pressure, Carotid Arteries, Cells, Cultured, Cellular Senescence, DNA Repair, DNA-Binding Proteins, Endonucleases, Endothelium, Vascular, Femoral Artery, Genomic Instability, Humans, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Models, Animal, Polymorphism, Single Nucleotide, Vascular Stiffness, Xeroderma Pigmentosum Group D Protein | null |
22,705,890 | 2012-10-25 | 2019-12-10 | 1361-6560 | Physics in medicine and biology | Evaluation of a novel triple-channel radiochromic film analysis procedure using EBT2. | van Hoof Stefan J, Granton Patrick V, Landry Guillaume, Podesta Mark, Verhaegen Frank | eng | null | Evaluation Study, Journal Article | null | IM | 22705890, 10.1088/0031-9155/57/13/4353 | A novel approach to read out radiochromic film was introduced recently by the manufacturer of GafChromic film. In this study, the performance of this triple-channel film dosimetry method was compared against the conventional single-red-channel film dosimetry procedure, with and without inclusion of a pre-irradiation (pre-IR) film scan, using EBT2 film and kilo- and megavoltage photon beams up to 10 Gy. When considering regions of interest averaged doses, the triple-channel method and both single-channel methods produced equivalent results. Absolute dose discrepancies between the triple-channel method, both single-channel methods and the treatment planning system calculated dose values, were no larger than 5 cGy for dose levels up to 2.2 Gy. Signal to noise in triple-channel dose images was found to be similar to signal to noise in single-channel dose images. The accuracy of resulting dose images from the triple- and single-channel methods with inclusion of pre-IR film scan was found to be similar. Results of a comparison of EBT2 data from a kilovoltage depth dose experiment to corresponding Monte Carlo depth dose data produced dose discrepancies of 9.5 ± 12 cGy and 7.6 ± 6 cGy for the single-channel method with inclusion of a pre-IR film scan and the triple-channel method, respectively. EBT2 showed to be energy sensitive at low kilovoltage energies with response differences of 11.9% and 15.6% in the red channel at 2 Gy between 50-225 kVp and 80-225 kVp photon spectra, respectively. We observed that the triple-channel method resulted in non-uniformity corrections of ±1% and consistency values of 0-3 cGy for the batches and dose levels studied. Results of this study indicate that the triple-channel radiochromic film read-out method performs at least as well as the single-channel method with inclusion of a pre-IR film scan, reduces film non-uniformity and saves time with elimination of a pre-IR film scan. | Film Dosimetry, Monte Carlo Method, Phantoms, Imaging, Photons, Software | null |
22,705,892 | 2013-03-26 | 2021-10-21 | 2165-5987 | Bioengineered | Extremotolerant fungi as genetic resources for biotechnology. | Gostinčar Cene, Turk Martina | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Phosphotransferases (Phosphomutases), Phosphoglucomutase, phosphomannomutase | IM | 22705892, 20713, 10.4161/bioe.20713, PMC3485746, 22363590, 17005001, 20723623, 8119301, 10858582, 3288631, 15687296, 7929458, 15483057, 19149734, 20630488, 9299700, 8472892, 11500487, 9252577, 18026957, 19756577, 20525638, 22386631, 21944213, 12702302 | Increased stress tolerance of economically important plants and microorganisms can improve yields in agriculture and industrial microbiology. The pool of resources used for the genetic modification of crops and industrial fungal strains in the past has been relatively limited, and has frequently included only stress-sensitive organisms. However, certain groups of fungi have evolved specialized mechanisms that enable them to thrive under even the most extreme of environmental conditions. These species can be considered as promising sources of biotechnologically interesting genes. Together with a powerful and convenient high-throughput functional screening method, extremotolerant fungi represent a new opportunity for the identification of stress-tolerance-conferring genes. The approaches described here should provide important contributions to the enhancing of the properties of economically important organisms in the future. | Biotechnology, Droughts, Gene Library, Genes, Fungal, Genetic Engineering, Phosphoglucomutase, Phosphotransferases (Phosphomutases), Phylogeny, Rhodotorula, Saccharomyces cerevisiae, Salinity, Salt Tolerance, Stress, Physiological | null |
22,705,895 | 2013-05-23 | 2013-11-21 | 1879-0712 | European journal of pharmacology | Investigation into the effect of the general anaesthetics etomidate and ketamine on long-range coupling of population activity in the mouse neocortical slice. | Voss Logan J, Baas Cecilia Hansson, Hansson Linnea, Steyn-Ross D Alistair, Steyn-Ross Moira, Sleigh James W | eng | null | Journal Article | Anesthetics, General, Ketamine, Etomidate | IM | 22705895, S0014-2999(12)00514-6, 10.1016/j.ejphar.2012.06.003 | General anaesthetics have been hypothesised to ablate consciousness by decoupling intracortical neural connectivity. We explored this by investigating the effect of etomidate and ketamine on coupling of neural population activity using the low magnesium neocortical slice model. Four extracellular electrodes (50 μm) were positioned in mouse neocortical slices (400 μm thick) with varying separation. The effect of etomidate (24 μM) and ketamine (16 μM) on the timing of population activity recorded between channels was analysed. No decoupling was observed at the closest electrode separation of 0.2 mm. At 4mm separation, decoupling was observed in 50% and 42% of slices during etomidate and ketamine delivery, respectively (P<0.0001 and P=0.002, compared to 0.2 mm separation). A lower rate of decoupling was observed with 1mm separation (21% and 8%, respectively, P<0.03 for etomidate compared to 0.2mm separation). The data support the hypothesis that mechanistically diverse general anaesthetics disrupt neuronal connectivity across widely distributed intracortical networks. | Action Potentials, Anesthetics, General, Animals, Etomidate, Female, Ketamine, Male, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Neocortex, Neurons, Organ Culture Techniques | null |
22,705,894 | 2013-05-23 | 2013-11-21 | 1879-0712 | European journal of pharmacology | Anti-cancer activity and mutagenic potential of novel copper(II) quinolinone Schiff base complexes in hepatocarcinoma cells. | Duff Brian, Thangella Venkat Reddy, Creaven Bernadette S, Walsh Maureen, Egan Denise A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, Quinolones, Schiff Bases, Copper | IM | 22705894, S0014-2999(12)00515-8, 10.1016/j.ejphar.2012.06.004 | This study determined the cytotoxic, cyto-selective and mutagenic potential of novel quinolinone Schiff base ligands and their corresponding copper(II) complexes in human-derived hepatic carcinoma cells (Hep-G2) and non-malignant human-derived hepatic cells (Chang). Results indicated that complexation of quinolinone Schiff bases with copper served to significantly enhance cytotoxicity. Here, the complex of (7E)-7-(3-ethoxy-2-hydroxybenzylideamino)-4-methylquinolin-2(1H)-one (TV117-FM) exhibited the lowest IC(50) value (17.9 μM) following 96 h continuous exposure, which was comparable to cisplatin (15.0 μM). However, results revealed that TV117-FM lacked cytoselectivity over non-malignant cells. Additionally, the complex was minimally effluxed from cells via Pglycoprotein (P-gp) and was shown to be non-mutagenic in the Standard Ames test. Furthermore, BrdU incorporation assays showed that it was capable of inhibiting DNA synthesis in a concentrationand time-dependent manner. However, inhibition was not as a consequence of DNA intercalation, as illustrated in electrophoretic mobility shift assays. Interestingly, it was shown that the ligand was capable of inhibiting the action of topoisomerase II, but this was lost following complexation. This indicated that the mechanism of action of the novel copper(II) complex was different from that of the parent ligand and suggests that TV117-FM may have a therapeutic role to play in the treatment of hepatocellular carcinoma. Studies are currently underway to elucidate the exact in vitro mechanism of action of this novel, metal-based anti-cancer agent. | Animals, Antineoplastic Agents, CHO Cells, Carcinoma, Hepatocellular, Cell Survival, Copper, Cricetinae, Cricetulus, HeLa Cells, Hep G2 Cells, Humans, Liver Neoplasms, Male, Mutagenesis, Quinolones, Rats, Rats, Sprague-Dawley, Schiff Bases | null |
22,705,891 | 2013-03-26 | 2021-10-21 | 2165-5987 | Bioengineered | Possible reasons for past failures of genetic engineering techniques for creating novel, xenobiotics-degrading bacteria. | Hernández-Sánchez Verónica, Wittich Regina-Michaela | eng | null | Letter, Research Support, Non-U.S. Gov't | DNA Transposable Elements, Xenobiotics | IM | 22705891, 20732, 10.4161/bioe.20732, PMC3477692, 20488990, 22337052, 22509013, 12727376, 17091349, 21261918, 8057911, 16200500, 17185547 | null | Bacteria, Biodegradation, Environmental, Conjugation, Genetic, DNA Transposable Elements, Gene Transfer Techniques, Genetic Engineering, Xenobiotics | null |
22,705,896 | 2012-12-17 | 2024-01-09 | 1879-0712 | European journal of pharmacology | Cycle arrest and apoptosis in MDA-MB-231/Her2 cells induced by curcumin. | Sun Shih-Han, Huang Hsiu-Chen, Huang Cheng, Lin Jen-Kun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, S-Phase Kinase-Associated Proteins, Cyclin-Dependent Kinase Inhibitor p27, ERBB2 protein, human, Receptor, ErbB-2, Proto-Oncogene Proteins c-akt, Extracellular Signal-Regulated MAP Kinases, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Curcumin | IM | 22705896, S0014-2999(12)00495-5, 10.1016/j.ejphar.2012.05.036 | Breast cancers with an overexpression of Her-2 or Skp2 overlap with those affected by reduced p27 expression. In breast cancer, the loss of p27 is associated with a poor prognosis. Curcumin, the main constituent of turmeric, has been found to stabilize p27 levels in breast cancer, but whether this effect is mediated through changes in Skp2 or Her2 expression remains unclear. This study investigates whether curcumin inhibits Skp2-mediated p27 ubiquitination in Her2/Skp2-overexpressing cancer cell lines (MDA-MB-231/Her2 cells). The results show that curcumin represses cell proliferation, induces G1 arrest at a lower dosage (30μM), triggers apoptosis at a higher dosage (50μM) and blocks cell migration in MDA-MB-231/Her2 cells. A low dose of curcumin increases p27 and decreases Skp2, Her2, Cyclin E, CDK kinases in a time- and dose-dependent manner, suggesting that p27, Skp2 and Her2 may be involved in the growth inhibition in MDA-MB-231/Her2 cells induced by curcumin. However, higher doses of curcumin produce a dose-dependent apoptotic death in MDA-MB-231/Her2 cells, which is related to cleaved forms of PARP and caspase 3. The findings indicate that curcumin is of potential value for the chemoprevention of breast cancer, especially in breast cancer with Skp2/Her2 overexpression. | Antineoplastic Agents, Apoptosis, Cell Cycle Checkpoints, Cell Line, Tumor, Cell Movement, Cell Proliferation, Curcumin, Cyclin-Dependent Kinase Inhibitor p27, Down-Regulation, Extracellular Signal-Regulated MAP Kinases, Gene Expression Regulation, Neoplastic, Humans, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Neoplasm Metastasis, Phosphorylation, Proto-Oncogene Proteins c-akt, Receptor, ErbB-2, S-Phase Kinase-Associated Proteins, Transcription, Genetic, Ubiquitination | null |
22,705,893 | 2013-03-26 | 2021-10-21 | 2165-5987 | Bioengineered | Baculovirus as a vaccine vector. | Lu Hsin-Yu, Chen Yi-Hsuan, Liu Hung-Jen | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Recombinant Proteins, Vaccines, Subunit | IM | 22705893, 20679, 10.4161/bioe.20679, PMC3477694, 15280475, 15877075, 17989172, 9185857, 17375072, 20018269, 19406162, 18809448, 19223476, 19446339, 12007867, 19604531, 9188571, 14561897, 20709008, 15568020, 15731258, 20392201, 17980465, 11145915, 9986796, 16405916, 19961961, 18727937, 10559295, 8676487, 8392598, 21793437, 2648574, 20727393, 11134303, 8159729, 18708107, 14671094, 22172969, 11423932, 19656899, 21115062, 19079592, 11831715, 16997010, 9811709, 18287233, 19474242, 9482726, 21197956, 18635031, 15709004, 16530857, 12922123, 8601317, 15464595, 16267571, 12941888, 7474124, 21092083 | Baculovirus is extensively utilized as an excellent tool for production of recombinant protein in insect cells. Baculovirus infects insects in nature and is non-pathogenic to humans. In addition to insect cells, baculovirus is capable of transducing a broad range of animal cells. Due to its biosafety, large cloning capacity, low cytotoxicity, and non-replication nature in the transduced cells as well as the ease of manipulation and production, baculovirus has been utilized as RNA interference mediators, gene delivery vectors, and vaccine vectors for a wide variety of applications. This article focuses on the utilization of baculoviruses as vaccine vectors to prepare antigen or subunit vaccines. | Animals, Baculoviridae, Cell Surface Display Techniques, Cells, Cultured, Gene Transfer Techniques, Genetic Therapy, Genetic Vectors, Humans, Insecta, RNA Interference, Recombinant Proteins, Transduction, Genetic, Vaccines, Subunit | null |
22,705,897 | 2012-10-25 | 2012-06-18 | 1538-9774 | Computers, informatics, nursing : CIN | Top 10 tips for teaching online. | Schwarz Laura Marie | eng | null | Journal Article | null | IM | 22705897, 10.1097/NXN.0b013e31825b374a, 00024665-201206000-00001 | null | Education, Distance, Education, Nursing, Baccalaureate, Education, Nursing, Graduate, Humans, Internet, Teaching | null |
22,705,898 | 2012-10-25 | 2012-06-18 | 1538-9774 | Computers, informatics, nursing : CIN | Hitech workforce development: a personal learning experience at Delgado Community College in Region D. | Estwick Angelyn B | eng | null | Journal Article | null | IM | 22705898, 10.1097/NXN.0b013e31825ee1a2, 00024665-201206000-00002 | null | Attitude of Health Personnel, Computer-Assisted Instruction, Education, Nursing, Associate, Faculty, Nursing, Humans | null |
22,705,902 | 2012-12-11 | 2012-08-03 | 1879-1026 | The Science of the total environment | Concentrations and source apportionment of PM10 and associated major and trace elements in the Rhodes Island, Greece. | Argyropoulos Georgios, Manoli Evangelia, Kouras Athanasios, Samara Constantini | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | null | 22705902, S0048-9697(12)00791-7, 10.1016/j.scitotenv.2012.05.076 | Ambient concentrations of PM(10) and associated major and trace elements were measured over the cold and the warm season of 2007 at two sites located in the Rhodes Island (Greece), in Eastern Mediterranean, aimed at source apportionment by Chemical Mass Balance (CMB) receptor modeling. Source chemical profiles, necessary in CMB modeling, were obtained for a variety of emission sources that could possibly affect the study area, including sea spray, geological material, soot emissions from the nearby oil-fuelled thermal power plant, and other anthropogenic activities, such as vehicular traffic, residential oil combustion, wood burning, and uncontrolled open-air burning of agricultural biomass and municipal waste. Source apportionment of PM(10) and elemental components was carried out by employing an advanced CMB version, the Robotic Chemical Mass Balance model (RCMB). Vehicular emissions were found to be major PM(10) contributor accounting, on average, for 36.8% and 31.7% during the cold period, and for 40.9% and 39.2% in the warm period at the two sites, respectively. The second largest source of ambient PM(10), with minor seasonal variation, was secondary sulfates (mainly ammonium and calcium sulfates), with total average contribution around 16.5% and 18% at the two sites. Soil dust was also a remarkable source contributing around 22% in the warm period, whereas only around 10% in the cold season. Soot emitted from the thermal power plant was found to be negligible contributor to ambient PM(10) (<1%), however it appeared to appreciably contribute to the ambient V and Ni (11.3% and 5.1%, respectively) at one of the sites during the warm period, when electricity production is intensified. Trajectory analysis did not indicate any transport of Sahara dust; on the contrary, long range transport of soil dust from arid continental regions of Minor Asia and of biomass burning aerosol from the countries surrounding the Black Sea was considered possible. | null | null |
22,705,901 | 2012-12-11 | 2019-12-10 | 1879-1026 | The Science of the total environment | Long-term dynamics of dissolved organic carbon: implications for drinking water supply. | Ledesma José L J, Köhler Stephan J, Futter Martyn N | eng | null | Evaluation Study, Journal Article | Drinking Water, Organic Chemicals, Carbon | IM | 22705901, S0048-9697(12)00753-X, 10.1016/j.scitotenv.2012.05.071 | Surface waters are the main source of drinking water in many regions. Increasing organic carbon concentrations are a cause for concern in Nordic countries since both dissolved and particulate organic carbon can transport contaminants and adversely affect drinking water treatment processes. We present a long-term study of dynamics of total (particulate and dissolved) organic carbon (TOC) concentrations in the River Fyris. This river supplies drinking water to approximately 200000 people in Uppsala, Sweden. The River Fyris is a main tributary to Lake Mälaren, which supplies drinking water to approximately 2 million people in the greater Stockholm area. Utilities responsible for drinking water supply in both Uppsala and Stockholm have expressed concerns about possible increases in TOC. We evaluate organic carbon dynamics within the Fyris catchment by calculating areal mass exports using observed TOC concentrations and modeled flows and by modeling dissolved organic carbon (as a proxy for TOC) using the dynamic, process based INCA-C model. Exports of TOC from the catchment ranged from 0.8 to 5.8 g m(-2) year(-1) in the period 1995-2010. The variation in annual exports was related to climatic variability which influenced seasonality and amount of runoff. Exports and discharge uncoupled at the end of 2008. A dramatic increase in TOC concentrations was observed in 2009, which gradually declined in 2010-2011. INCA-C successfully reproduced the intra- and inter-annual variation in concentrations during 1996-2008 and 2010-2011 but failed to capture the anomalous increase in 2009. We evaluated a number of hypotheses to explain the anomaly in 2009 TOC values, ultimately none proved satisfactory. We draw two main conclusions: there is at least one unknown or unmeasured process controlling or influencing surface water TOC and INCA-C can be used as part of the decision-making process for current and future use of rivers for drinking water supply. | Carbon, Climate, Computer Simulation, Drinking Water, Environmental Monitoring, Models, Theoretical, Organic Chemicals, Rivers, Seasons, Sweden, Water Movements | null |
22,705,904 | 2012-12-11 | 2017-11-16 | 1879-1026 | The Science of the total environment | Hydrologic control on redox and nitrogen dynamics in a peatland soil. | Rubol Simonetta, Silver Whendee L, Bellin Alberto | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Soil, Water, Nitrous Oxide, Nitrogen, Oxygen | IM | 22705904, S0048-9697(12)00755-3, 10.1016/j.scitotenv.2012.05.073 | Soils are a dominant source of nitrous oxide (N(2)O), a potent greenhouse gas. However, the complexity of the drivers of N(2)O production and emissions has hindered our ability to predict the magnitude and spatial dynamics of N(2)O fluxes. Soil moisture can be considered a key driver because it influences oxygen (O(2)) supply, which feeds back on N(2)O sources (nitrification versus denitrification) and sinks (reduction to dinitrogen). Soil water content is directly linked to O(2) and redox potential, which regulate microbial metabolism and chemical transformations in the environment. Despite its importance, only a few laboratory studies have addressed the effects of hydrological transient dynamics on nitrogen (N) cycling in the vadose zone. To further investigate these aspects, we performed a long term experiment in a 1.5 m depth soil column supplemented by chamber experiments. With this experiment, we aimed to investigate how soil moisture dynamics influence redox sensitive N cycling in a peatland soil. As expected, increased soil moisture lowered O(2) concentrations and redox potential in the soil. The decline was more severe for prolonged saturated conditions than for short events and at deep than at the soil surface. Gaseous and dissolved N(2)O, dissolved nitrate (NO(3)(-)) and ammonium (NH(4)(+)) changed considerably along the soil column profile following trends in soil O(2) and redox potential. Hot spots of N(2)O concentrations corresponded to high variability in soil O(2) in the upper and lower parts of the column. Results from chamber experiments confirmed high NO(3)(-) reduction potential in soils, particularly from the bottom of the column. Under our experimental conditions, we identified a close coupling of soil O(2) and N(2)O dynamics, both of which lagged behind soil moisture changes. These results highlight the relationship among soil hydrologic properties, redox potential and N cycling, and suggest that models working at a daily scale need to consider soil O(2) dynamics in addition to soil moisture dynamics to accurately predict patterns in N(2)O fluxes. | California, Chromatography, Gas, Environmental Monitoring, Nitrogen, Nitrous Oxide, Oxidation-Reduction, Oxygen, Rain, Seasons, Soil, Water, Water Movements | null |
22,705,903 | 2012-12-11 | 2013-11-21 | 1879-1026 | The Science of the total environment | Fluoride content in drinking water supply in São Miguel volcanic island (Azores, Portugal). | Cordeiro S, Coutinho R, Cruz J V | eng | null | Journal Article | Drinking Water, Fluorides | IM | 22705903, S0048-9697(12)00752-8, 10.1016/j.scitotenv.2012.05.070 | High fluoride contents in the water supply of the city of Ponta Delgada, located in the volcanic island of São Miguel (Azores, Portugal) have been reported. Dental fluorosis in São Miguel has been identified and described in several medical surveys. The water supply in Ponta Delgada consists entirely of groundwater. A study was carried out in order to characterize the natural F-pollution of a group of springs (30) and wells (3), that are associated to active central volcanoes of a trachytic nature. Two springs known for their high content in fluoride were sampled, both located in the central volcano of Furnas. The sampled waters are cold, ranging from slightly acidic to slightly alkaline (pH range 6.53-7.60), exhibiting a low electrical conductivity (springs range 87-502 μS/cm; wells range 237-1761 μS/cm), and are mainly from the Na-HCO(3), Na-HCO(3)-Cl and Na-Cl-HCO(3) water types. Results suggest two main trends of geochemical evolution: silicate weathering, enhanced by CO(2) dilution, and seawater spraying. Fluoride contents range between 0.17 mg/L and 2 mg/L, and no seasonal variations were detected. Results in the sources of the water supply system are lower than those of the Furnas volcano, which reach 5.09 mgF/L, demonstrating the effect of F-rich gaseous emanations in this area. Instead, the higher fluoride contents in the water supply are mainly due to silicate weathering in aquifers made of more evolved volcanic rocks. | Azores, Chromatography, Ion Exchange, Drinking Water, Environmental Monitoring, Fluorides, Groundwater, Portugal, Spectrophotometry, Atomic, Water Supply | null |
22,705,906 | 2012-12-04 | 2013-11-21 | 1364-5528 | The Analyst | A molecularly imprinted photonic polymer sensor with high selectivity for tetracyclines analysis in food. | Wang Li-Qing, Lin Fan-Yan, Yu Li-Ping | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Colloids, Polystyrenes, Tetracycline, Chlortetracycline, Oxytetracycline | IM | 22705906, 10.1039/c2an35460h | A molecularly imprinted photonic polymer (MIPP) sensor for respective detection of tetracycline, oxytetracycline and chlortetracycline is developed based on the combination of a colloidal crystal templating method and a molecular imprinting technique. Colloidal crystal templates are prepared from monodisperse polystyrene colloids. The molecularly imprinted polymer, which is embodied in the colloidal crystal templates, is synthesized with acrylic acid and acrylamide as monomers, N,N'-methylene bisacrylamide as a cross-linker and tetracyclines (TCs) as imprinting template molecules. After removal of the colloidal crystal template and the molecularly imprinted template, the resulted MIPP consists of a three-dimensional, highly ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. The response of MIPP to TCs stimulants in aqueous solution is detected through a readable Bragg diffraction red-shift, which is due to the lattice change of MIPP structures responding to their rebinding to the target TCs molecules. A linear relationship was found between the Δλ and the concentration of TCs in the range from 0.04 μM to 0.24 μM. With this sensory system, direct and selective detection of TCs has been achieved without using label techniques and expensive instruments. The developed method has been applied successfully to detect tetracycline in milk and honey samples. | Animals, Chlortetracycline, Colloids, Food Analysis, Honey, Milk, Molecular Imprinting, Molecular Structure, Oxytetracycline, Particle Size, Polystyrenes, Surface Properties, Tetracycline | null |
22,705,905 | 2012-12-04 | 2015-11-19 | 1364-5528 | The Analyst | Surface functionalization chemistries on highly sensitive silica-based sensor chips. | Gopinath Subash C B, Awazu Koichi, Fujimaki Makoto, Shimizu Kazufumi, Mizutani Wataru, Tsukagoshi Kiyomi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Imidazoles, N,N-carbonyldiimidazole, Silicon Dioxide, Streptavidin, Glutaral | IM | 22705905, 10.1039/c2an35159e | The surfaces of silica-based sensor chips, designed for evanescent-field-coupled waveguide-mode sensors, were functionalized using various surface chemistries. The immobilization of molecular entities on the functionalized silica surfaces was monitored using various microscopic techniques (scanning electron, fluorescence, and atomic force microscopies). Further, gold nanoparticle-based signal enhancement analyses were performed with protein conjugation on different functionalized surfaces using a waveguide-mode sensor. Based on these analyses, the sensor surfaces modified with glutaraldehyde (Glu) and carbonyldiimidazole were found to be good for molecules of different sizes. In addition, it can be inferred that the Glu-modified surface may be suitable for small molecules with diameters around 5 nm owing to its surface roughness. The modified surface with carbonyldiimidazole is suitable for the direct immobilization of larger molecules especially for biomolecular assemblies without intermediate chemical modifications. | Glutaral, Imidazoles, Microscopy, Fluorescence, Particle Size, Protein Array Analysis, Silicon Dioxide, Streptavidin, Surface Properties | null |
22,705,907 | 2012-11-09 | 2013-11-21 | 1872-7972 | Neuroscience letters | Bone marrow stromal cells induce cell cycle arrest in reactive astrocytes in vitro. | Cai Kefu, Xu Jun, Zhang Yingdong | eng | null | Journal Article | Nitric Oxide, Canavanine, Nitric Oxide Synthase Type II | IM | 22705907, S0304-3940(12)00813-0, 10.1016/j.neulet.2012.06.017 | Transplantation of bone marrow stromal cells (BMSCs) reduces astrogliosis, decreases scar thickness and improves neurological functional recovery after brain damage. It is believed that transplanted BMSCs have a profound influence on astrocytes. To obtain the possible mechanism in their interaction, a co-culture system between BMSCs and astrocytes were set to investigate whether BMSCs could modulate cell cycle machinery in reactive astrocytes. The results obtained showed cell cycle regulatory proteins, cdk4 along with its activator cyclin D1, and PCNA increased while p27, an endogenous cyclin-dependent kinase inhibitor, deceased in glutamate-treated astrocytes in vitro. However, BMSCs influenced cell cycle elements in the cocultured astrocytes: cyclin D1, cdk 4 and PCNA were downregulated, while p27 was unregulated. Flow cytometry showed astrocytes in the S phase after glutamate incubation increased to 17.4±2.0% while restored to a level of 7.8±1.1% when cocultured with BMSCs. l-Canavanine, an inhibitor of inducible nitric oxide synthase, partially reversed the S phase to 11.3±0.4% in the cocultured astrocytes. These data indicated that BMSCs might inhibit the cell cycle control system in reactive astrocytes and nitric oxide signaling was involved in this process. The decline of astrogliosis conferred by BMSCs may derive from their effect of inhibiting the cell cycle progression in astrocytes. | Animals, Astrocytes, Bone Marrow Cells, Canavanine, Cell Cycle Checkpoints, Cells, Cultured, Coculture Techniques, Nitric Oxide, Nitric Oxide Synthase Type II, Rats, Rats, Sprague-Dawley, Stromal Cells | null |
22,705,908 | 2012-11-09 | 2019-12-10 | 1872-7972 | Neuroscience letters | Spatial and non-spatial performance in mutant mice devoid of otoliths. | Machado M L, Kroichvili N, Freret T, Philoxène B, Lelong-Boulouard V, Denise P, Besnard S | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705908, S0304-3940(12)00812-9, 10.1016/j.neulet.2012.06.016 | Vestibular deafferentation induces strong spatial memory impairments in rodents and dorsal hippocampal atrophy in humans, suggesting that vestibular information plays an important role in spatial-memory processes. However, previous studies have not discriminated between the role of the semi-circular canals, gravisensors and cochlear sense organ in such impairments due to complete damage of the vestibular and cochlear organs in their models of lesions. This is the first time that mutant mice (het/het) devoid of otoconia (lack of vestibular gravisensors) have been evaluated in behavioral tests. Results show different levels of achievement in the tests. The rotarod and elevated plus-maze were not executable, the rotarod being a safer test for differentiating the het/het mouse phenotype compared to the more anxiogenic swimming pool. Y-maze and place recognition tests were achieved, but chance values were not reached in the het/het group. Additionally, het/het mice presented uncommon behavior when faced with objects during the object recognition test. Impairments in het/het mice in the Y-maze test suggest a crucial role of the vestibular gravisensors in spatial-memory processes. | Animals, Male, Maze Learning, Memory, Mice, Mice, Mutant Strains, Otolithic Membrane, Recognition, Psychology, Rotarod Performance Test, Space Perception | null |
22,705,909 | 2012-11-09 | 2017-11-16 | 1872-7972 | Neuroscience letters | Effect of chronic activation of 5-HT3 receptors on 5-HT3, 5-HT(1A) and 5-HT(2A) receptors functional activity and expression of key genes of the brain serotonin system. | Kondaurova Elena M, Naumenko Vladimir S, Popova Nina K | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biguanides, Receptor, Serotonin, 5-HT2A, Receptors, Serotonin, 5-HT3, Serotonin 5-HT3 Receptor Agonists, Receptor, Serotonin, 5-HT1A, 1-(3-chlorophenyl)biguanide | IM | 22705909, S0304-3940(12)00811-7, 10.1016/j.neulet.2012.06.015 | Among serotonin (5-HT) receptors, the 5-HT(3) receptor is the only ligand-gated ion-channel. Little is known about the interaction between the 5-HT(3) receptor and other 5-HT receptors and influence of 5-HT(3) chronic activation on other 5-HT receptors and the expression of key genes of 5-HT system. Chronic activation of 5-HT(3) receptor with intracerebroventricularly administrated selective agonist 1-(3-chlorophenyl)biguanide hydrochloride (m-CPBG) (14 days, 40 nmol, i.c.v.) produced significant desensitization of 5-HT(3) and 5-HT(1A) receptors. The hypothermic responses produced by acute administration of selective agonist of 5-HT(3) receptor (m-CPBG, 40 nmol, i.c.v.) or selective agonist of 5-HT(1A) receptor (8-hydroxy-2-(di-n-propylamino)tetralin) (8-OH-DPAT, 1mg/kg, i.p.) was significantly lower in m-CPBG treated mice compared with the mice of control groups. Chronic m-CPBG administration failed to induce any significant change in the 5-HT(2A) receptor functional activity and in the expression of the gene encoding 5-HT(2A) receptor. Chronic activation of 5-HT(3) receptor produced no considerable effect on the expression on 5-HT(3), 5-HT(1A), and 5-HT transporter (5-HTT) and tryptophan hydroxylase-2 (TPH-2) genes - the key genes of brain 5-HT system, in the midbrain, frontal cortex and hippocampus. In conclusion, chronic activation of ionotropic 5-HT(3) receptor produced significant desensitization of 5-HT(3) and postsynaptic 5-HT(1A) receptors but caused no considerable changes in the expression of key genes of the brain 5-HT system. | Animals, Biguanides, Brain, Mice, Mice, Inbred Strains, Receptor, Serotonin, 5-HT1A, Receptor, Serotonin, 5-HT2A, Receptors, Serotonin, 5-HT3, Serotonin 5-HT3 Receptor Agonists | null |
22,705,910 | 2012-12-13 | 2013-11-21 | 1878-0180 | Journal of the mechanical behavior of biomedical materials | Mechanical properties and in vitro bioactivity of injectable and self-setting calcium sulfate/nano-HA/collagen bone graft substitute. | Hu Ning-Min, Chen Zonggang, Liu Xi, Liu Huanye, Lian Xiaojie, Wang Xiumei, Cui Fu-Zhai | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biocompatible Materials, Bone Substitutes, nano-hydroxyapatite-collagen, Collagen, Durapatite, Calcium Sulfate | IM | 22705910, S1751-6161(11)00308-0, 10.1016/j.jmbbm.2011.12.007 | Calcium sulfate hemihydrate (CSH) was introduced into the mineralized collagen (nHAC) to prepare an injectable and self-setting in situ bone graft substitute. The mechanical properties of materials, which are dependant on the L/S ratio, the content of nHAC and setting accelerator, were discussed based on the satisfying injectability and setting properties. It was found that the compressive strength and modulus of materials increased with the decrease of nHAC content and L/S ratio. CSD as setting accelerator hardly had an effect on the compressive properties of materials because it is not only the reactant of preparing CSH but also the final solidified product of CSH instead of a foreign body. Though the compressive properties of nHAC/CSD composites changed with the variety of nHAC content and L/S ratio, the compressive strength and modulus of the materials ranged from 2.0 to nearly 20.0 MPa and 100.0 to 800.0 MPa, respectively, which are similar to that of cancellous bone. In vitro cell behavior demonstrated that the composites could provide adequate environment for cell adhesion and proliferation. All these indicated that the nHAC/CSH composites were a potential scaffold for bone tissue engineering. | Animals, Biocompatible Materials, Biomechanical Phenomena, Bone Substitutes, Bone and Bones, Calcium Sulfate, Cell Proliferation, Collagen, Compressive Strength, Durapatite, Injections, Materials Testing, Microscopy, Electron, Scanning, Pressure, Rabbits, Stress, Mechanical, Tissue Engineering | null |
22,705,911 | 2012-12-14 | 2013-11-21 | 1872-7573 | Journal of ethnopharmacology | Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich ascites carcinoma treated mice. | Dolai Narayan, Karmakar Indrajit, Suresh Kumar R B, Kar Biswakanth, Bala Asis, Haldar Pallab Kanti | eng | null | Journal Article | Antineoplastic Agents, Phytogenic, Antioxidants, Plant Extracts, Catalase, Superoxide Dismutase, Glutathione | IM | 22705911, S0378-8741(12)00372-8, 10.1016/j.jep.2012.05.050 | Anthocephalus cadamba (Roxb.) Miq. (Family: Rubiaceae) is commonly known as "Kadamba" in Sanskrit and Hindi in India. Various parts of this plant have been used as a folk medicine for the treatment of tumor, wound healing, inflammation and as a hypoglycemic agent. | Animals, Antineoplastic Agents, Phytogenic, Antioxidants, Carcinoma, Ehrlich Tumor, Catalase, Cell Line, Tumor, Glutathione, Mice, Phytotherapy, Plant Bark, Plant Extracts, Rubiaceae, Superoxide Dismutase, Tumor Burden | null |
22,705,913 | 2013-03-20 | 2021-10-21 | 1089-8611 | Nitric oxide : biology and chemistry | A nanoparticle delivery vehicle for S-nitroso-N-acetyl cysteine: sustained vascular response. | Nacharaju Parimala, Tuckman-Vernon Chaim, Maier Keith E, Chouake Jason, Friedman Adam, Cabrales Pedro, Friedman Joel M | eng | P01 HL071064 (NHLBI NIH HHS, United States); P01 HL110900 (NHLBI NIH HHS, United States); R01 HL052684 (NHLBI NIH HHS, United States) | Journal Article | Drug Carriers, S-Nitrosothiols, Carbon Dioxide, Hydrogel, Polyethylene Glycol Dimethacrylate, S-nitroso-N-acetylcysteine, S-Nitrosoglutathione, Glutathione, Oxygen, Acetylcysteine | IM | 22705913, S1089-8603(12)00261-3, 10.1016/j.niox.2012.06.003, PMC4156139, NIHMS612819, 7525649, 12956902, 21539916, 12727142, 1956043, 8533007, 11843150, 1346070, 3196352, 12220879, 2784476, 20460149, 3242600, 19387479, 8689564, 11807184, 8594612, 21946032, 1768273, 18457680, 6115052, 7067094, 18158484, 21178416, 19663720, 7526102, 19915659, 20203313, 12084001, 2175799, 8684748, 21510727, 2873150, 6720909, 15544469, 7733655, 22286699, 12954813, 16198610, 21057038, 19089342, 7634823, 8527258, 3311265, 14551038, 18658051, 6135148, 9193701, 15638781, 16101422, 18209572, 15688001, 8025915, 3126076, 21161638, 2511992, 21577055, 3829378, 21342744, 11139363, 12826064, 9630353, 8828915, 19726230, 12175821, 19765204, 16095564, 10781381, 16609997, 11749666, 17025266, 1449475, 7903392, 18443613, 11433215, 12636927, 8425188, 22149535, 21748357, 9726628, 9731637, 19026385, 7732599 | Interest in the development of nitric oxide (NO) based therapeutics has grown exponentially due to its well elucidated and established biological functions. In line with this surge, S-nitroso thiol (RSNO) therapeutics are also receiving more attention in recent years both as potential stable sources of NO as well as for their ability to serve as S-nitrosating agents; S-nitrosation of protein thiols is implicated in many physiological processes. We describe two hydrogel based RSNO containing nanoparticle platforms. In one platform the SNO groups are covalently attached to the particles (SNO-np) and the other contains S-nitroso-N-acetyl cysteine encapsulated within the particles (NAC-SNO-np). Both platforms function as vehicles for sustained activity as trans-S-nitrosating agents. NAC-SNO-np exhibited higher efficiency for generating GSNO from GSH and maintained higher levels of GSNO concentration for longer time (24 h) as compared to SNO-np as well as a previously characterized nitric oxide releasing platform, NO-np (nitric oxide releasing nanoparticles). In vivo, intravenous infusion of the NAC-SNO-np and NO-np resulted in sustained decreases in mean arterial pressure, though NAC-SNO-np induced longer vasodilatory effects as compared to the NO-np. Serum chemistries following infusion demonstrated no toxicity in both treatment groups. Together, these data suggest that the NAC-SNO-np represents a novel means to both study the biologic effects of nitrosothiols and effectively capitalize on its therapeutic potential. | Acetylcysteine, Animals, Blood Pressure, Carbon Dioxide, Cricetinae, Drug Carriers, Glutathione, Heart Rate, Hydrogel, Polyethylene Glycol Dimethacrylate, Male, Mesocricetus, Nanoparticles, Oxygen, S-Nitrosoglutathione, S-Nitrosothiols, Vasodilation | null |
22,705,917 | 2012-08-13 | 2018-10-23 | 1756-1833 | BMJ (Clinical research ed.) | London Olympics are unlikely to have beneficial public health legacy, peers hear. | O'Dowd Adrian | eng | null | News | null | IM | 22705917, 10.1136/bmj.e4202 | null | England, Health Promotion, Humans, London, Public Health, Sports, Sports Medicine | null |
22,705,916 | 2013-12-30 | 2018-12-02 | 1471-8405 | Occupational medicine (Oxford, England) | Systematic review: Progression of beryllium sensitization to chronic beryllium disease. | Seidler A, Euler U, Müller-Quernheim J, Gaede K I, Latza U, Groneberg D, Letzel S | eng | null | Journal Article, Review, Systematic Review | Air Pollutants, Occupational, Radioisotopes, Beryllium | IM | 22705916, kqs069, 10.1093/occmed/kqs069 | The relevance of beryllium sensitization testing for occupational health practice and prevention is unclear. | Air Pollutants, Occupational, Berylliosis, Beryllium, Bronchoalveolar Lavage Fluid, Chronic Disease, Disease Progression, Female, Germany, Humans, Lymphocyte Activation, Male, Occupational Diseases, Occupational Exposure, Prognosis, Radioisotopes | null |
22,705,915 | 2012-11-01 | 2022-04-08 | 2042-0226 | Cellular & molecular immunology | New insights of T cells in the pathogenesis of psoriasis. | Cai Yihua, Fleming Chris, Yan Jun | eng | null | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | Interleukin-17, Receptors, Antigen, T-Cell, gamma-delta | IM | 22705915, cmi201215, 10.1038/cmi.2012.15, PMC4132586, 19283718, 17291186, 8251347, 19682928, 22455412, 21796151, 16323244, 11380148, 10393847, 18521187, 19641206, 19684581, 20393462, 19609313, 21984702, 17658397, 19487306, 20430306, 14698226, 10571730, 21982596, 20016504, 17900301, 17603494, 7836926, 14871644, 2847872, 17291283, 11422184, 19648274, 17074928, 17676044, 10540348, 7561119, 18021893, 17658398, 19471949, 21339323, 21536803, 19682929, 21813772, 18200064, 8106745, 11114383, 17045843, 22362575, 7937756, 15491424, 21654831, 17277128, 19578369, 16493599, 17201677, 18454150, 20032993, 22131335, 1677144, 1739285, 18684158, 11084303, 11710947, 21606249, 17290272, 10974034, 19675575, 19578368, 20112373, 16619290, 18633443, 11472417, 22455413, 20483798, 19781993, 8878440, 15373909, 19687432, 20363977, 9201310, 22455420, 12950680, 16982811, 19132915, 14510985, 16424222, 18802076, 21574984, 1677143, 193433, 7529261, 19161421, 17187052, 22277938, 19662682, 15611238, 10751418, 19182808, 7593594, 15145319, 10469066, 20053323, 20071701, 18663128, 11142774, 20580412, 6136547, 20430301, 10837080, 21172868, 19537380, 22011907, 21894442, 21124836, 14707118, 20926833, 19920355, 10328195, 460314 | Psoriasis is one of the most common immune-mediated chronic, inflammatory skin diseases characterized by hyperproliferative keratinocytes and infiltration of T cells, dendritic cells, macrophages and neutrophils. Although the pathogenesis of psoriasis is not fully understood, there is ample evidence suggesting that the dysregulation of immune cells in the skin, particularly T cells, plays a critical role in psoriasis development. In this review, we mainly focus on the pathogenic T cells and discuss how these T cells are activated and involved in the disease pathogenesis. Newly identified 'professional' IL-17-producing dermal γδ T cells and their potential role in psoriasis will also be included. Finally, we will briefly summarize the recent progress on the T cell and its related cytokine-targeted therapy for psoriasis treatment. | Animals, Cell Movement, Cell Proliferation, Humans, Interleukin-17, Keratinocytes, Molecular Targeted Therapy, Psoriasis, Receptors, Antigen, T-Cell, gamma-delta, T-Lymphocytes | null |
22,705,914 | 2012-11-01 | 2022-03-21 | 2042-0226 | Cellular & molecular immunology | Generation of natural killer cells from hematopoietic stem cells in vitro for immunotherapy. | Luevano Martha, Madrigal Alejandro, Saudemont Aurore | eng | null | Journal Article, Review | null | IM | 22705914, cmi201217, 10.1038/cmi.2012.17, PMC4012863, 8920872, 3489775, 18978771, 3528289, 21245483, 18036444, 8547640, 21173117, 3284289, 9573023, 21698239, 300411, 12407407, 10561265, 10587352, 16936306, 22031859, 12242449, 15800326, 10687148, 17013389, 21715685, 11917118, 18641363, 1080480, 21375422, 19553639, 11146169, 16902150, 18836917, 16291655, 7688785, 19660715, 9804556, 10648413, 15457184, 21531164, 11830464, 15331438, 21039469, 19365083, 18155552, 18273053, 17100876, 18295962, 3088121, 18192509, 20169160, 11841430, 7654433, 2471777, 16079848, 2783573, 11940227, 3494538, 21757615, 21248224, 7551914, 15831091, 21812652, 11896281, 21351269, 16210613, 10411505, 18458113, 15528141, 9816022, 7585540, 8639878, 19995955, 3951539, 10560675, 50294, 10381530, 15274367, 8938105, 8493555, 1086218, 11698225, 9192756, 19749763, 15705423, 9348320, 9209487, 12766763, 9057360, 15755898, 11964270, 21073262, 12637322, 12415255, 21555534, 20085940, 16901727, 17999637, 20636021, 7513206, 19383914, 17600469, 18493613, 15780987, 7540065, 12393617, 11342442, 20795758, 1698553, 22143670, 19103877, 15632206, 18714017, 9843216, 20491532, 16946036, 11468170, 20696944, 9850851, 21304407, 19712464, 9808558, 12068293, 1668282, 8616068, 20890071, 8077657, 1234049, 8409465, 16551250 | Natural killer (NK) cells are part of the innate immune system and are an alluring option for immunotherapy due to their ability to kill infected cells or cancer cells without prior sensitization. Throughout the past 20 years, different groups have been able to reproduce NK cell development in vitro, and NK cell ontogeny studies have provided the basis for the establishment of protocols to produce NK cells in vitro for immunotherapy. Here, we briefly discuss NK cell development and NK cell immunotherapy approaches. We review the factors needed for NK cell differentiation in vitro, which stem cell sources have been used, published protocols, challenges and future directions for Good Manufacturing Practice protocols. | Animals, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cytotoxicity, Immunologic, Hematopoietic Stem Cells, Humans, Immunity, Innate, Immunotherapy, Adoptive, Infections, Killer Cells, Natural, Neoplasms, Stem Cell Niche | null |
22,705,918 | 2012-08-13 | 2018-12-01 | 1756-1833 | BMJ (Clinical research ed.) | Transfer of public health to local authorities could leave 60 top posts vacant, survey finds. | Limb Matthew | eng | null | News | null | IM | 22705918, 10.1136/bmj.e4205 | null | England, Humans, Leadership, Personnel Turnover, Public Health Administration, State Medicine, Workforce | null |
22,705,912 | 2013-03-29 | 2025-01-03 | 1873-3379 | Research in developmental disabilities | Loneliness among students with special educational needs in mainstream seventh grade. | Bossaert Goele, Colpin Hilde, Pijl Sip Jan, Petry Katja | eng | null | Comparative Study, Journal Article | null | IM | 22705912, S0891-4222(12)00126-6, 10.1016/j.ridd.2012.05.010 | The goals of this study were twofold. The first aim was to explore loneliness prevalence in typically developing students, students with ASD and students with motor and/or sensory disabilities in mainstream 7th grade in Belgium. The second aim was to explore the relations between number of friends, friendship quality, social self-concept on the one hand and loneliness on the other for each of these three groups, and to compare them across groups. In this study, 108 students with special educational needs (SEN; i.e., 58 students with ASD and 50 students with motor and/or sensory disabilities) were matched to 108 typically developing classmates. Students with ASD reported more loneliness than typically developing students and students with motor and/or sensory disabilities. Loneliness prevalence for typically developing students and students with motor and/or sensory disabilities did not differ significantly. Factors related with loneliness differed between typically developing students and students with SEN (i.e., students with ASD and students with motor and/or sensory disabilities). For students with SEN, same-sex social self-concept was related with loneliness, but not, as for typically developing students, number of friends and opposite-sex social self-concept. Also friendship quality had a marginally significant effect on loneliness feelings for students with SEN. Implications for further research and practice are discussed. | Adolescent, Child, Child Development Disorders, Pervasive, Children with Disabilities, Education, Special, Female, Friends, Gender Identity, Humans, Intellectual Disability, Loneliness, Mainstreaming, Education, Male, Reference Values, Self Concept, Social Adjustment, Sociometric Techniques, Surveys and Questionnaires | null |
22,705,919 | 2013-03-12 | 2015-11-19 | 1532-0987 | The Pediatric infectious disease journal | Practice patterns of infectious disease physicians for management of meningococcal disease. | Terranella Andrew, Beekmann Susan E, Polgreen Philip M, Cohn Amanda, Wu Henry M, Clark Thomas A | eng | U50 CI 00358 (NCPDCID CDC HHS, United States) | Journal Article, Research Support, U.S. Gov't, P.H.S. | Anti-Bacterial Agents | IM | 22705919, 10.1097/INF.0b013e31826323a4 | Although empiric treatment regimens for acute bacterial meningitis are well established, there are many uncertainties regarding management of meningococcal disease. A survey was conducted among infectious disease specialists to assess meningococcal disease practice patterns and availability of antimicrobial susceptibility testing for Neisseria menigitidis. | Anti-Bacterial Agents, Data Collection, Drug Resistance, Bacterial, Humans, Infectious Disease Medicine, Meningococcal Infections, Neisseria meningitidis, Practice Patterns, Physicians', Surveys and Questionnaires | null |
22,705,921 | 2012-11-26 | 2012-07-24 | 1872-8359 | Journal of microbiological methods | Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. | Koh Seng Fook, Tay Sun Tee, Sermswan Rasana, Wongratanacheewin Surasakdi, Chua Kek Heng, Puthucheary Savithri D | eng | null | Journal Article, Research Support, Non-U.S. Gov't | RNA, Bacterial, RNA, Ribosomal, 16S | IM | 22705921, S0167-7012(12)00215-1, 10.1016/j.mimet.2012.06.002 | We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks. | Burkholderia cepacia complex, Burkholderia mallei, Burkholderia pseudomallei, Electrophoresis, Agar Gel, Genes, Bacterial, Molecular Typing, Multiplex Polymerase Chain Reaction, RNA, Bacterial, RNA, Ribosomal, 16S | null |
22,705,922 | 2012-11-26 | 2013-11-21 | 1872-8359 | Journal of microbiological methods | Detection and validation of a small broad-host-range plasmid pBBR1MCS-2 for use in genetic manipulation of the extremely acidophilic Acidithiobacillus sp. | Hao Likai, Liu Xiangmei, Wang Huiyan, Lin Jianqun, Pang Xin, Lin Jianqiang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Streptomycin | IM | 22705922, S0167-7012(12)00216-3, 10.1016/j.mimet.2012.06.003 | An efficient genetic system for introducing genes into biomining microorganisms is essential not only to experimentally determine the functions of genes predicted based on bioinformatic analysis, but also for their genetic breeding. In this study, a small broad-host-range vector named pBBR1MCS-2, which does not belong to the IncQ, IncW, or IncP groups, was studied for the feasibility of its use in conjugative gene transfer into extremely acidophilic strains of Acidithiobacillus. To do this, a recombinant plasmid pBBR-tac-Sm, a derivative of pBBR1MCS-2, was constructed and the streptomycin resistant gene (Sm(r)) was used as the reporter gene. Using conjugation, pBBR-tac-Sm was successfully transferred into three tested strains of Acidithiobacillus. Then we measured its transfer frequency, its stability in Acidithiobacillus cells, and the level of resistance to streptomycin of the transconjugants and compared this with the IncQ plasmid pJRD215 control. Our results indicate that pBBR1MCS-2 provides a new and useful tool in the genetic manipulation of Acidithiobacillus strains. | Acidithiobacillus thiooxidans, Anti-Bacterial Agents, Cloning, Molecular, Conjugation, Genetic, Drug Resistance, Bacterial, Escherichia coli, Gene Transfer, Horizontal, Genes, Reporter, Genetic Engineering, Plasmids, Species Specificity, Streptomycin | null |
22,705,923 | 2012-09-21 | 2013-11-21 | 1090-2449 | Experimental parasitology | Prevention of Schistosoma japonicum infection in mice with long-acting praziquantel implants. | Lei Lei, Cheng Liang, Hou Jingwen, Guo Shengrong, Zhu Chuangang, Shi Yaojun, Jiang Yan, Lin Jiaojiao | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anthelmintics, Drug Implants, Praziquantel | IM | 22705923, S0014-4894(12)00177-4, 10.1016/j.exppara.2012.05.016 | This work reports the prevention outcomes of a praziquantel (PZQ) implant against the infection of Schistosoma japonicum in mice. The PZQ implant produced stable plasma PZQ concentrations in a range of 100-1300 ng/mL for a period of 70 days, by releasing PZQ in subcutaneous tissues in a sustained manner. To assess the prevention effects, the mice were infected at varying times after implantation. All the mice were sacrificed at 6 weeks after infection for worm and egg recovery and counting, worm morphological examination, determination of egg-hatching rates, and analysis of hepatic histology. The infection was successfully prevented for mice with early infection times (within 2-3 weeks), as nearly no worms, paired worms, eggs, or miracidia were recovered. However, in mice with late infection times (after 3 weeks), the prevention effects were diminished due to the decreased plasma PZQ concentrations at late times. Interestingly, the implants showed robust prevention effects on repeated infection at 1 and 3 weeks. In the infection-prevented mouse livers, no granuloma formation or granulomatous inflammation was observed. The results demonstrated that by blocking the development of infecting miracidia and by deactivating the eggs, the PZQ implants encouragingly prevented the S. japonicum infection and avoided liver damage. | Animals, Anthelmintics, Drug Implants, Female, Liver, Male, Mice, Microscopy, Electron, Scanning, Praziquantel, Random Allocation, Schistosoma japonicum, Schistosomiasis japonica, Time Factors | null |
22,705,924 | 2013-01-14 | 2021-10-21 | 1559-2308 | Epigenetics | DNA methylation analyses of urothelial carcinoma reveal distinct epigenetic subtypes and an association between gene copy number and methylation status. | Lauss Martin, Aine Mattias, Sjödahl Gottfrid, Veerla Srinivas, Patschan Oliver, Gudjonsson Sigurdur, Chebil Gunilla, Lövgren Kristina, Fernö Mårten, Månsson Wiking, Liedberg Fredrik, Ringnér Markus, Lindgren David, Höglund Mattias | eng | null | Journal Article, Research Support, Non-U.S. Gov't | ATF2 protein, human, Activating Transcription Factor 2, EZH2 protein, human, Enhancer of Zeste Homolog 2 Protein, Polycomb Repressive Complex 2 | IM | 22705924, 20837, 10.4161/epi.20837, PMC3427281, 19151715, 10802651, 22284968, 21659424, 16203795, 20193056, 16979735, 15616584, 21788354, 19329998, 20219944, 21770893, 11395428, 12149476, 17575217, 17785556, 22122642, 16396632, 17613547, 18237450, 20565864, 18478570, 16630818, 20802236, 19542153, 20406976, 16432078, 17200670, 20841482, 21430268, 21173382, 21118553, 16093321, 19750229, 20830311, 20808801, 15930339, 21059933, 18292213, 21825015, 17099711, 17334365, 18196590, 15352172, 21706001, 16646809, 21533174, 17234643, 19458158, 17953745 | We assessed DNA methylation and copy number status of 27,000 CpGs in 149 urothelial carcinomas and integrated the findings with gene expression and mutation data. Methylation was associated with gene expression for 1,332 CpGs, of which 26% showed positive correlation with expression, i.e., high methylation and high gene expression levels. These positively correlated CpGs were part of specific transcription factor binding sites, such as sites for MYC and CREBP1, or located in gene bodies. Furthermore, we found genes with copy number gains, low expression and high methylation levels, revealing an association between methylation and copy number levels. This phenomenon was typically observed for developmental genes, such as HOX genes, and tumor suppressor genes. In contrast, we also identified genes with copy number gains, high expression and low methylation levels. This was for instance observed for some keratin genes. Tumor cases could be grouped into four subgroups, termed epitypes, by their DNA methylation profiles. One epitype was influenced by the presence of infiltrating immune cells, two epitypes were mainly composed of non-muscle invasive tumors, and the remaining epitype of muscle invasive tumors. The polycomb complex protein EZH2 that blocks differentiation in embryonic stem cells showed increased expression both at the mRNA and protein levels in the muscle invasive epitype, together with methylation of polycomb target genes and HOX genes. Our data highlights HOX gene silencing and EZH2 expression as mechanisms to promote a more undifferentiated and aggressive state in UC. | Activating Transcription Factor 2, Carcinoma, CpG Islands, DNA Methylation, Enhancer of Zeste Homolog 2 Protein, Epigenesis, Genetic, Gene Dosage, Genes, Homeobox, Genes, Tumor Suppressor, Genes, myc, Humans, Polycomb Repressive Complex 2, Urinary Bladder Neoplasms, Urothelium | null |
22,705,925 | 2012-10-25 | 2012-06-20 | 1361-6560 | Physics in medicine and biology | Evaluation of nuclear reaction cross-sections and fragment yields in carbon beams using the SHIELD-HIT Monte Carlo code. Comparison with experiments. | Hultqvist Martha, Lazzeroni Marta, Botvina Alexander, Gudowska Irena, Sobolevsky Nikolai, Brahme Anders | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Carbon | IM | 22705925, 10.1088/0031-9155/57/13/4369 | In light ion therapy, the knowledge of the spectra of both primary and secondary particles in the target volume is needed in order to accurately describe the treatment. The transport of ions in matter is complex and comprises both atomic and nuclear processes involving primary and secondary ions produced in the cascade of events. One of the critical issues in the simulation of ion transport is the modeling of inelastic nuclear reaction processes, in which projectile nuclei interact with target nuclei and give rise to nuclear fragments. In the Monte Carlo code SHIELD-HIT, inelastic nuclear reactions are described by the Many Stage Dynamical Model (MSDM), which includes models for the different stages of the interaction process. In this work, the capability of SHIELD-HIT to simulate the nuclear fragmentation of carbon ions in tissue-like materials was studied. The value of the parameter κ, which determines the so-called freeze-out volume in the Fermi break-up stage of the nuclear interaction process, was adjusted in order to achieve better agreement with experimental data. In this paper, results are shown both with the default value κ = 1 and the modified value κ = 10 which resulted in the best overall agreement. Comparisons with published experimental data were made in terms of total and partial charge-changing cross-sections generated by the MSDM, as well as integral and differential fragment yields simulated by SHIELD-HIT in intermediate and thick water targets irradiated with a beam of 400 MeV u(-1) (12)C ions. Better agreement with the experimental data was in general obtained with the modified parameter value (κ = 10), both on the level of partial charge-changing cross-sections and fragment yields. | Carbon, Monte Carlo Method, Nuclear Energy | null |
22,705,926 | 2012-10-03 | 2016-11-25 | 1468-201X | Heart (British Cardiac Society) | Neutrophil gelatinase-associated lipocalin, cystatin C and eGFR indicate acute kidney injury and predict prognosis of patients with acute pulmonary embolism. | Kostrubiec Maciej, Łabyk Andrzej, Pedowska-Włoszek Justyna, Dzikowska-Diduch Olga, Wojciechowski Artur, Garlińska Marzena, Ciurzyński Michał, Pruszczyk Piotr | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acute-Phase Proteins, Biomarkers, CST3 protein, human, Cystatin C, LCN2 protein, human, Lipocalin-2, Lipocalins, Proto-Oncogene Proteins, Creatinine | IM | 22705926, heartjnl-2012-301884, 10.1136/heartjnl-2012-301884 | Risk stratification in acute pulmonary embolism (APE) includes the assessment of clinical status, right ventricular dysfunction and troponin concentrations. Since acute renal impairment is one of the important predictors of mortality in cardiovascular diseases, the authors hypothesised that it is an independent mortality marker in APE. | Acute Disease, Acute Kidney Injury, Acute-Phase Proteins, Aged, Aged, 80 and over, Biomarkers, Chi-Square Distribution, Creatinine, Cystatin C, Female, Glomerular Filtration Rate, Humans, Kaplan-Meier Estimate, Kidney, Lipocalin-2, Lipocalins, Male, Middle Aged, Multidetector Computed Tomography, Multivariate Analysis, Poland, Predictive Value of Tests, Prognosis, Proportional Hazards Models, Prospective Studies, Proto-Oncogene Proteins, Pulmonary Embolism, ROC Curve, Risk Assessment, Risk Factors, Sensitivity and Specificity, Time Factors, Ventricular Function, Right | null |
22,705,927 | 2012-12-03 | 2012-09-24 | 1468-201X | Heart (British Cardiac Society) | Diagnosing peri-procedural myocardial injury following percutaneous coronary intervention: replacing confusion with consensus. | van Gaal William J, Banning Adrian P | eng | null | Editorial | null | IM | 22705927, heartjnl-2012-302131, 10.1136/heartjnl-2012-302131 | null | Consensus, Humans, Myocardial Infarction, Percutaneous Coronary Intervention | null |
22,705,929 | 2012-10-02 | 2018-12-01 | 1468-201X | Heart (British Cardiac Society) | Important cost categories not included: transcatheter aortic valve implantation probably less cost-effective. | van Baal Pieter | eng | null | Letter, Research Support, Non-U.S. Gov't, Comment | null | IM | 22705929, heartjnl-2012-302272, 10.1136/heartjnl-2012-302272 | null | Aortic Valve Stenosis, Cardiac Catheterization, Heart Valve Prosthesis Implantation, Humans, Models, Economic | null |
22,705,930 | 2013-07-29 | 2018-12-01 | 1468-201X | Heart (British Cardiac Society) | Comparison between cardiac troponin T and creatinine kinase MB mass in the diagnosis of myocardial infarction. | Goi Leise Sckenal, Sturmer Giovani | eng | null | Letter, Comment | Myoglobin, Troponin I, Creatine Kinase, MB Form | IM | 22705930, heartjnl-2012-302206, 10.1136/heartjnl-2012-302206 | null | Chest Pain, Creatine Kinase, MB Form, Early Diagnosis, Female, Humans, Male, Myocardial Infarction, Myoglobin, Troponin I | null |
22,705,934 | 2012-10-12 | 2016-07-19 | 1477-9234 | Dalton transactions (Cambridge, England : 2003) | Convenient C-alkylation of the [HCB11Cl11]- carborane anion. | Ramírez-Contreras Rodrigo, Ozerov Oleg V | eng | null | Journal Article | null | null | 22705934, 10.1039/c2dt12431a | A method for C-alkylation of [HCB(11)Cl(11)](-) anions using potassium tert-butoxide and alkyl iodides in tert-butanol is presented. Trimethylammonium salts of the corresponding C-alkylated derivatives are easily isolated and obtained in good yields. | null | null |
22,705,933 | 2012-10-03 | 2016-11-25 | 1468-201X | Heart (British Cardiac Society) | A patient with pulmonary embolism and a mass in the left atrium. | Nägele Reto, Bonetti Piero Ovidio, Mayer Kurt Albert | eng | null | Case Reports, Journal Article | null | IM | 22705933, heartjnl-2011-301445, 10.1136/heartjnl-2011-301445 | null | Aged, Cardiac Surgical Procedures, Echocardiography, Doppler, Embolism, Paradoxical, Female, Heart Atria, Heart Neoplasms, Heart Septal Defects, Atrial, Humans, Myxoma, Pulmonary Embolism, Treatment Outcome | null |
22,705,935 | 2012-10-23 | 2022-03-31 | 1873-4367 | Colloids and surfaces. B, Biointerfaces | The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. | Dipankar C, Murugan S | eng | null | Journal Article | Anti-Bacterial Agents, Antioxidants, Plant Extracts, Silver | IM | 22705935, S0927-7765(12)00199-3, 10.1016/j.colsurfb.2012.04.006 | Silver has been used since time immemorial in different chemical form to treat burns, wounds and several infections caused by pathogenic bacteria. Advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the synthesis, characterization of silver nanoparticles using Iresine herbstii and evaluation of their antibacterial, antioxidant and cytotoxic activity. The reaction mixture turned to brownish gray color after 7 days of incubation and exhibits an absorbance peak around 460 nm characteristic of Ag nanoparticle. Scanning electron microscopy (SEM) and EDX analysis showed silver nanoparticles were pure and polydispersed and the size were ranging from 44 to 64 nm. X-ray diffraction (XRD) studies revealed that most of the nanoparticles were cubic and face centered cubic in shape. Fourier transform infrared spectroscopy (FTIR) showed nanoparticles were capped with plant compounds. Biosynthesized silver nanoparticles showed potent antibacterial activity against human pathogenic bacteria. Phytosynthesized nanoparticles exhibited strong antioxidant activity as well as cytotoxicity against HeLa cervical cell lines. The approach of green synthesis seems to be cost efficient, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis. The powerful bioactivity demonstrated by the synthesized silver nanoparticles leads towards the clinical use as antibacterial, antioxidant as well as cytotoxic agent. | Amaranthaceae, Anti-Bacterial Agents, Antioxidants, Metal Nanoparticles, Microscopy, Electron, Scanning, Plant Extracts, Plant Leaves, Silver, X-Ray Diffraction | null |
22,705,936 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | Synchronous primary epithelial tumors of the pancreas. | Karidis Nikolaos P, Paraskeva Panoraia, Mantas Dimitrios | eng | null | Journal Article | null | null | 22705936, S2210-2612(12)00103-4, 10.1016/j.ijscr.2012.05.009, PMC3397287, 1805050, 15968256, 18376323, 17124434, 10767715, 16861136, 9287984, 1933654, 7074553, 16084460, 18053836, 11141506, 18040629 | Pancreatic incidentalomas are diagnosed at increased rates due to advanced pancreatic imaging. Coexistence of such lesions with another pancreatic pathology, however, is uncommon and their management might be perplexed by the anatomical location and the histological features of the lesion. | null | null |
22,705,937 | 2012-10-02 | 2022-03-17 | 2210-2612 | International journal of surgery case reports | A case of vulval swelling secondary to female circumcision posing a diagnostic dilemma. | Amu Okwudili C, Udeh Emeka I, Ugochukwu Anthony I, Madu Chukwudi, Nzegwu Martin A | eng | null | Journal Article | null | null | 22705937, S2210-2612(12)00090-9, 10.1016/j.ijscr.2012.03.038, PMC3397301, 9310217, 7474278, 21534352, 7295598, 8058079, 3739335, 20236635, 9276655, 9061394, 1286628, 6879696, 7767932, 7787654, 10743534, 8654884 | The practice of FGM is most prevalent in the African countries such as Nigeria, Ethiopia, Sudan, Egypt, and some area of the Middle East. It is not restricted to any ethnic, religious or socioeconomic class. There are many reasons for perpetuation of this practice; the most common are cultural and religious beliefs. The aim of this paper is to highlight the diagnostic dilemma associated with this type of case and the psychological trauma of a patient following her unfortunate genital mutilations. | null | null |
22,705,938 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | A large epidermoid cyst of breast mimicking carcinoma: A case report and review of literature. | Debnath Debasish, Taribagil Savita, Al-Janabi Khalid J S, Inwang Reggie | eng | null | Journal Article | null | null | 22705938, S2210-2612(12)00080-6, 10.1016/j.ijscr.2012.03.037, PMC3397304, 421003, 8092018, 21221490, 3602368, 21132291, 18057439, 19299100, 8416547, 16328396, 17856704, 6744168, 19970719, 10901863 | Triple assessment of a suspicious breast lesion may not always provide a definite diagnosis. We report a case of epidermoid cyst of breast, which caused diagnostic dilemma in spite of a thorough triple assessment and entailed mastectomy. | null | null |
22,705,940 | 2013-03-12 | 2019-12-10 | 0006-3002 | Biochimica et biophysica acta | Trimethylamine N-oxide suppresses the activity of the actomyosin motor. | Kumemoto Ryusei, Yusa Kento, Shibayama Tomohiro, Hatori Kuniyuki | eng | null | Evaluation Study, Journal Article | Methylamines, Multiprotein Complexes, Myosin Subfragments, Urea, Actomyosin, Adenosine Triphosphatases, trimethyloxamine | IM | 22705940, S0304-4165(12)00173-0, 10.1016/j.bbagen.2012.06.006 | During actomyosin interactions, the transduction of energy from ATP hydrolysis to motility seems to occur with the modulation of hydration. Trimethylamine N-oxide (TMAO) perturbs the surface of proteins by altering hydrogen bonding in a manner opposite to that of urea. Hence, we focus on the effects of TMAO on the motility and ATPase activation of actomyosin complexes. | Actin Cytoskeleton, Actomyosin, Adenosine Triphosphatases, Animals, Dose-Response Relationship, Drug, Down-Regulation, In Vitro Techniques, Methylamines, Movement, Multiprotein Complexes, Myosin Subfragments, Osmolar Concentration, Protein Stability, Rabbits, Urea | null |
22,705,941 | 2013-03-12 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | Type III and V collagens modulate the expression and assembly of EDA(+) fibronectin in the extracellular matrix of defective Ehlers-Danlos syndrome fibroblasts. | Zoppi Nicoletta, Ritelli Marco, Colombi Marina | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Collagen Type III, Collagen Type V, Fibronectins, Integrin alpha5beta1, Integrins, Protein Isoforms, extra domain A fibronectin, human, integrin alpha 9 beta 1, Focal Adhesion Kinase 1, PTK2 protein, human | IM | 22705941, S0304-4165(12)00171-7, 10.1016/j.bbagen.2012.06.004 | Alternative splicing of EDA fibronectin (FN) region is a cell type- and development-regulated mechanism controlled by pathological processes, growth factors and extracellular matrix (ECM). Classic and vascular Ehlers-Danlos syndrome (cEDS and vEDS) are connective tissue disorders caused by COL5A1/COL5A2 and COL3A1 gene mutations, leading to an in vivo abnormal collagen fibrillogenesis and to an in vitro defective organisation in the ECM of type V (COLLV) and type III collagen (COLLIII). These defects induce the FN-ECM disarray and the decrease of COLLs and FN receptors, the α2β1 and α5β1 integrins. Purified COLLV and COLLIII restore the COLL-FN-ECMs in both EDS cell strains. | Case-Control Studies, Cells, Cultured, Collagen Type III, Collagen Type V, Ehlers-Danlos Syndrome, Extracellular Matrix, Female, Fibroblasts, Fibronectins, Focal Adhesion Kinase 1, Gene Expression Regulation, Humans, Integrin alpha5beta1, Integrins, Male, Protein Isoforms, Protein Multimerization | null |
22,705,943 | 2013-03-14 | 2021-10-21 | 0006-3002 | Biochimica et biophysica acta | Hematopoietic stem cell development and regulatory signaling in zebrafish. | Zhang Chunxia, Patient Roger, Liu Feng | eng | MC_U137981013 (Medical Research Council, United Kingdom) | Journal Article, Research Support, Non-U.S. Gov't, Review | null | IM | 22705943, S0304-4165(12)00175-4, 10.1016/j.bbagen.2012.06.008 | Hematopoietic stem cells (HSCs) are a population of multipotent cells that can self-renew and differentiate into all blood lineages. HSC development must be tightly controlled from cell fate determination to self-maintenance during adulthood. This involves a panel of important developmental signaling pathways and other factors which act synergistically within the HSC population and/or in the HSC niche. Genetically conserved processes of HSC development plus many other developmental advantages make the zebrafish an ideal model organism to elucidate the regulatory mechanisms underlying HSC programming. | Animals, Body Patterning, Cell Differentiation, Hematopoietic Stem Cells, Zebrafish | null |
22,705,939 | 2012-10-12 | 2012-06-18 | 1538-9804 | Cancer nursing | Mindful movement program for older breast cancer survivors: a pilot study. | Crane-Okada Rebecca, Kiger Holly, Sugerman Fred, Uman Gwen C, Shapiro Shauna L, Wyman-McGinty Wendy, Anderson Nancy L R | eng | P30NR005041 (NINR NIH HHS, United States) | Journal Article, Randomized Controlled Trial, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | null | IM | 22705939, 10.1097/NCC.0b013e3182280f73, 00002820-201207000-00011 | Older breast cancer survivors (BCSs) are at risk for late and long-term treatment effects on quality of life (QOL), including lower physical functioning and fear of recurrence. Two promising approaches to address this include dance/movement therapy and mindfulness. | Aged, Aged, 80 and over, Breast Neoplasms, Community-Based Participatory Research, Dance Therapy, Feasibility Studies, Female, Humans, Middle Aged, Mind-Body Therapies, Movement, Nursing Methodology Research, Pilot Projects, Program Evaluation, Quality of Life, Survivors, Treatment Outcome | null |
22,705,942 | 2013-03-14 | 2021-10-21 | 0006-3002 | Biochimica et biophysica acta | Signal transduction pathways, intrinsic regulators, and the control of cell fate choice. | Fossett Nancy | eng | R01 DK072229 (NIDDK NIH HHS, United States); DK072229 (NIDDK NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | null | IM | 22705942, S0304-4165(12)00172-9, 10.1016/j.bbagen.2012.06.005, PMC3477240, NIHMS386546, 17361183, 15998795, 11597829, 20023157, 7796812, 20483458, 10861002, 15314643, 10700180, 16248995, 14512024, 28305337, 12930778, 22036566, 14979663, 9550723, 17361184, 19737914, 17936744, 19483695, 7700357, 15286786, 20876645, 19062086, 17404248, 18056420, 19212321, 12024038, 10330188, 6776413, 9927674, 15659345, 16810242, 12805946, 17418114, 9553047, 8662508, 16730345, 14602069, 11404479, 12032775, 15199955, 8479437, 12374748, 11714753, 15659837, 18603010, 28305338, 20689801, 19641625, 20010697, 19773069, 17976521, 9160668, 16094372, 12100886, 15857916, 21660044, 10353918, 11161576, 15082553, 19261847, 11018018, 21295568, 20707995, 22407365, 20535209, 1765005, 11846478, 19460351, 9367989, 19498372, 18779318, 8078582, 20485470, 17869239, 17507674, 9927675, 16794031, 11971000, 20182459, 21615335, 15659346, 14504400, 19648026, 18815369, 15205520, 23213407, 10078204, 14738767, 18371424, 21381183, 19727075, 11262875, 11731473, 20110496, 16277982, 9367990, 10980429, 10753120, 16551245, 7729418, 21133842, 21203476, 9230307, 22331866, 20670937, 11798069, 19855015, 22196733, 17962413, 17934481, 18299398, 18334252, 15659348, 21566654, 12077323 | Information regarding changes in organismal status is transmitted to the stem cell regulatory machinery by a limited number of signal transduction pathways. Consequently, these pathways derive their functional specificity through interactions with stem cell intrinsic master regulators, notably transcription factors. Identifying the molecular underpinnings of these interactions is critical to understanding stem cell function. | Animals, Cell Lineage, Drosophila, Signal Transduction | null |
22,705,944 | 2012-09-24 | 2021-12-03 | 1460-2075 | The EMBO journal | Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state. | Kojer Kerstin, Bien Melanie, Gangel Heike, Morgan Bruce, Dick Tobias P, Riemer Jan | eng | null | Journal Article | MIA40 protein, S cerevisiae, Mitochondrial Membrane Transport Proteins, Mitochondrial Precursor Protein Import Complex Proteins, Saccharomyces cerevisiae Proteins, Glutathione Reductase, Glutathione | IM | 22705944, emboj2012165, 10.1038/emboj.2012.165, PMC3400016, 21478822, 14672937, 9580605, 21865594, 12584194, 22093747, 2482359, 2045415, 12727869, 1680311, 11278619, 17445698, 11027572, 1523409, 8662189, 15989955, 20367280, 15364952, 18786560, 17967948, 20188670, 19703392, 10212186, 16246122, 20450883, 16915356, 11689426, 18469822, 17253982, 15701801, 15359280, 18708636, 14985369, 20019331, 19026441, 20088706, 20404317, 19498160, 10620338, 12702463, 9435273, 17892447, 20555384, 20530571, 15277542, 17845131, 19720617, 10037727, 14722062, 15224968, 15507438, 11434918, 20346371, 21378306, 19158096, 21964034, 9559853, 19477928, 3860816 | Glutathione is an important mediator and regulator of cellular redox processes. Detailed knowledge of local glutathione redox potential (E(GSH)) dynamics is critical to understand the network of redox processes and their influence on cellular function. Using dynamic oxidant recovery assays together with E(GSH)-specific fluorescent reporters, we investigate the glutathione pools of the cytosol, mitochondrial matrix and intermembrane space (IMS). We demonstrate that the glutathione pools of IMS and cytosol are dynamically interconnected via porins. In contrast, no appreciable communication was observed between the glutathione pools of the IMS and matrix. By modulating redox pathways in the cytosol and IMS, we find that the cytosolic glutathione reductase system is the major determinant of E(GSH) in the IMS, thus explaining a steady-state E(GSH) in the IMS which is similar to the cytosol. Moreover, we show that the local E(GSH) contributes to the partially reduced redox state of the IMS oxidoreductase Mia40 in vivo. Taken together, we provide a comprehensive mechanistic picture of the IMS redox milieu and define the redox influences on Mia40 in living cells. | Cytosol, Glutathione, Glutathione Reductase, Mitochondria, Mitochondrial Membrane Transport Proteins, Mitochondrial Precursor Protein Import Complex Proteins, Oxidation-Reduction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins | null |
22,705,945 | 2012-10-25 | 2021-10-21 | 1460-2075 | The EMBO journal | Silent decision: HP1 protein escorts heterochromatic RNAs to their destiny. | Ren Jie, Martienssen Robert A | eng | R01 GM076396 (NIGMS NIH HHS, United States) | Journal Article, Comment | Chromosomal Proteins, Non-Histone, Heterochromatin, Schizosaccharomyces pombe Proteins, RNA | IM | 22705945, emboj2012172, 10.1038/emboj.2012.172, PMC3411077, 18025105, 14983173, 22002604, 11722841, 17512405, 12560555, 19111658, 15071554, 17173056, 22683269 | null | Chromosomal Proteins, Non-Histone, Gene Expression Regulation, Heterochromatin, RNA, Schizosaccharomyces, Schizosaccharomyces pombe Proteins | null |
22,705,947 | 2012-12-14 | 2012-07-23 | 1522-9629 | Pulmonary pharmacology & therapeutics | Immunomodulatory effects of feeding with Bifidobacterium longum on allergen-induced lung inflammation in the mouse. | MacSharry John, O'Mahony Caitlin, Shalaby Karim Hamdy, Sheil Barbara, Karmouty-Quintana Harry, Shanahan Fergus, Martin James G | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Allergens, Inflammation Mediators, Ovalbumin | IM | 22705947, S1094-5539(12)00076-4, 10.1016/j.pupt.2012.05.011 | The intestinal microbiota has important effects on host immune responses and feeding with certain commensal organisms has anti-inflammatory effects in a variety of diseases, including experimental asthma. The aim of the current study was to examine how robust the effects of feeding with the commensal strain, Bifidobacterium longum (Bif) were on the pulmonary responses to allergen sensitization and challenge. | Allergens, Animals, Bifidobacterium, Bronchial Hyperreactivity, Bronchoalveolar Lavage Fluid, Disease Models, Animal, Inflammation Mediators, Male, Mice, Mice, Inbred BALB C, Ovalbumin, Pneumonia, Probiotics | null |
22,705,946 | 2012-10-25 | 2022-03-09 | 1460-2075 | The EMBO journal | Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release. | Malsam Jörg, Parisotto Daniel, Bharat Tanmay A M, Scheutzow Andrea, Krause Jean Michel, Briggs John A G, Söllner Thomas H | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Adaptor Proteins, Vesicular Transport, Nerve Tissue Proteins, Synaptotagmin I, Unilamellar Liposomes, complexin I, Calcium | IM | 22705946, emboj2012164, 10.1038/emboj.2012.164, PMC3411073, 7592870, 17891149, 18275379, 8422678, 16845390, 19716167, 16912714, 19179400, 19540234, 16888141, 11163241, 7935764, 7954835, 21307261, 21145004, 19033464, 8917590, 11562488, 21215631, 18784080, 18618940, 12110842, 11754837, 19164750, 16782782, 18552825, 7971978, 11242035, 17873870, 11832227, 21785413, 17906638, 19571812, 22184197, 7284312, 7553862, 22447935, 19132534, 11823420, 22407297, 18205405, 21344950, 15911881, 16987956, 15919191, 12055633, 12645522, 20448186, 11739399, 10692432, 15340165, 12119360, 16642042, 19914185, 17828276, 20819981, 18369953, 1642895, 9759724, 18726177, 7659162, 15217342, 18499660, 12388083, 16794037, 14709554, 17110340, 18496517, 11413542, 20133592, 21705659, 12213837, 16528727, 9570782, 8455717, 9529252, 18505837, 12805548, 14718921, 10600482, 10570484, 9861047, 17478680, 9742137, 21785414, 18622390, 19164751 | Regulated exocytosis requires that the assembly of the basic membrane fusion machinery is temporarily arrested. Synchronized membrane fusion is then caused by a specific trigger--a local rise of the Ca(2+) concentration. Using reconstituted giant unilamellar vesicles (GUVs), we have analysed the role of complexin and membrane-anchored synaptotagmin 1 in arresting and synchronizing fusion by lipid-mixing and cryo-electron microscopy. We find that they mediate the formation and consumption of docked small unilamellar vesicles (SUVs) via the following sequence of events: Synaptotagmin 1 mediates v-SNARE-SUV docking to t-SNARE-GUVs in a Ca(2+)-independent manner. Complexin blocks vesicle consumption, causing accumulation of docked vesicles. Together with synaptotagmin 1, complexin synchronizes and stimulates rapid fusion of accumulated docked vesicles in response to physiological Ca(2+) concentrations. Thus, the reconstituted assay resolves both the stimulatory and inhibitory function of complexin and mimics key aspects of synaptic vesicle fusion. | Adaptor Proteins, Vesicular Transport, Biological Transport, Calcium, Exocytosis, Humans, In Vitro Techniques, Membrane Fusion, Models, Biological, Nerve Tissue Proteins, Protein Binding, Synaptic Transmission, Synaptic Vesicles, Synaptotagmin I, Time Factors, Unilamellar Liposomes | null |
22,705,948 | 2013-04-25 | 2013-11-21 | 1522-9629 | Pulmonary pharmacology & therapeutics | Hydrogen peroxide and nitrite reduction in exhaled breath condensate of COPD patients. | Stefanska J, Sarniak A, Wlodarczyk A, Sokolowska M, Doniec Z, Bialasiewicz P, Nowak D, Pawliczak R | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acetophenones, Nitrites, Nitric Oxide, acetovanillone, Hydrogen Peroxide | IM | 22705948, S1094-5539(12)00074-0, 10.1016/j.pupt.2012.06.001 | Chronic obstructive pulmonary disease (COPD) is predominantly the result of years of cigarette smoking. Increased oxidative stress in COPD derives from the increased burden of inhaled oxidants (cigarette smoke), air pollution and the increase in reactive oxygen and nitrogen species (ROS and RNS), generated by some inflammatory, immune, and structural airways cells. In view of the lack of therapy that might inhibit the progress of the disease, there is an urgent need for a successful therapeutic approach. Apocynin is a molecule inhibiting activation of NADPH oxidase - enzyme generating ROS and RNS precursor. Thus, our aim was to analyze apocynin influence on hydrogen peroxide and nitrite concentrations in EBC of COPD patients. Apocynin reduced concentration of H(2)O(2) in COPD patients 60 and 120 min after apocynin inhalation, in comparison to placebo (0.43 μM vs. 0.59 μM, and 0.4 μM vs. 0.59 μM respectively, p < 0.05). Moreover, apocynin decreased NO(2)(-) ions concentration in airways of COPD patients after apocynin nebulization (3.97 μM vs. 4.48 μM after 30 min, 3.82 μM vs. 4.48 μM after 60 min, and 3.76 μM vs. 4.48 μM after 30 min respectively, p < 0.05). No adverse effects have been observed. The results suggest that apocynin might be considered as anti-inflammatory agent, and, possibly used in therapy of COPD. | Acetophenones, Adult, Aged, Breath Tests, Cross-Over Studies, Double-Blind Method, Female, Humans, Hydrogen Peroxide, Male, Middle Aged, Nitric Oxide, Nitrites, Oxidation-Reduction, Pulmonary Disease, Chronic Obstructive | null |
22,705,954 | 2012-12-13 | 2024-06-10 | 1873-7897 | Journal of anxiety disorders | Avoidant personality disorder in individuals with generalized social anxiety disorder: what does it add? | Marques Luana, Porter Eliora, Keshaviah Aparna, Pollack Mark H, Van Ameringen Michael, Stein Murray B, Simon Naomi M | eng | R01 MH070919 (NIMH NIH HHS, United States); R01MH70917 (NIMH NIH HHS, United States); R01MH70501 (NIMH NIH HHS, United States); K23 MH096029 (NIMH NIH HHS, United States); R01 MH070917 (NIMH NIH HHS, United States); R01 MH070501 (NIMH NIH HHS, United States); R01MH70919 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705954, S0887-6185(12)00069-2, 10.1016/j.janxdis.2012.05.004, PMC3417304, NIHMS387389, 10077308, 9348491, 11043888, 16905400, 20455606, 12086222, 9203111, 8290681, 9234464, 16095476, 17161000, 8040509, 11722301, 17560956, 1583226, 10960264, 19031488, 9881538, 10372342, 10834623, 1583228, 16324903, 8923116, 2885745, 9153683, 2081237, 11722303, 2280326 | Avoidant personality disorder (AvPD) has a high level of symptom overlap and comorbidity with generalized social anxiety disorder (GSAD). We examined whether the presence of comorbid AvPD adds significant clinically relevant information for individuals seeking treatment for GSAD. Results suggested that AvPD was significantly associated with poorer quality of life and greater disability in univariate, but not multivariate analyses. Endorsement of more AvPD symptoms was associated with increased disability, increased risk of intimacy, and lower social support, even after covariate adjustment. Specifically, AvPD item 3, hard to be "open" even with people you are close to, was most strongly correlated with quality of life and disability. A binary diagnosis of AvPD alone adds little beyond a marker of greater GSAD severity and depression among patients with GSAD, while a specific feature of AvPD not captured by the GSAD diagnosis, namely emotional guardedness, may be associated with greater impairment. | Adult, Antisocial Personality Disorder, Comorbidity, Female, Humans, Interpersonal Relations, Male, Mental Health, Middle Aged, Phobic Disorders, Self Concept, Severity of Illness Index, Social Adjustment, Socioeconomic Factors, Surveys and Questionnaires, Young Adult | null |
22,705,949 | 2012-12-21 | 2024-06-10 | 1873-4596 | Free radical biology & medicine | Selective binding of nuclear alpha-synuclein to the PGC1alpha promoter under conditions of oxidative stress may contribute to losses in mitochondrial function: implications for Parkinson's disease. | Siddiqui Almas, Chinta Shankar J, Mallajosyula Jyothi K, Rajagopolan Subramanian, Hanson Ingrid, Rane Anand, Melov Simon, Andersen Julie K | eng | R01 NS045615 (NINDS NIH HHS, United States); RL1 NS062415 (NINDS NIH HHS, United States); R01NS045615 (NINDS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Chromatin, Heat-Shock Proteins, PPARGC1A protein, human, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Transcription Factors, alpha-Synuclein, Electron Transport Complex I | IM | 22705949, S0891-5849(12)00269-9, 10.1016/j.freeradbiomed.2012.05.024, PMC3418424, NIHMS387308, 19125866, 20733075, 20655384, 15947785, 19526285, 19915576, 8965721, 16837598, 17275196, 20070850, 17275140, 17055439, 19279012, 16854430, 9749577, 19542216, 9582300, 19697948, 15076727, 19626387, 20599975, 21486936, 21296890, 20178833, 20340137, 19628769, 19475667, 20926834, 16959795, 19555051, 21376232, 20380693, 19915575, 17588900, 18366718, 18074631, 2566813, 11125145, 12963742, 12859192, 20701279, 20887775, 20628651, 18286173, 17485113, 18417705, 19771175 | Alpha-synuclein has been reported to be present in the nucleus and levels enhanced by oxidative stress. Herein, we sought to investigate the mechanistic role of nuclear alpha-synuclein. We found that alpha-synuclein nuclear localization coincided with enhanced chromatin binding both in an in vitro and a corresponding in vivo brain oxidative stress model previously characterized by our laboratory as well as in PD brain tissues. Genome-wide chromatin immunoprecipitation (ChIP)-on-chip analysis of alpha-synuclein:promoter binding in response to oxidative stress in vitro revealed that binding occurs at several promoters belonging to a range of functional categories including transcriptional regulation. Interestingly, given the important role of mitochondrial dysfunction in PD, this included binding to the promoter for the master mitochondrial transcription activator, PGC1alpha in vitro, in vivo, and in human brain tissue with age and PD. To test the possible mechanistic impact of alpha-synuclein PGC1alpha promotor binding, we assessed PGC1alpha promoter activity, mRNA, and protein levels and expression of candidate PGC1alpha target genes in our in vitro model. All were found to be reduced in conjunction with increased levels of aberrant mitochondrial morphology and impaired mitochondrial function. Exogenous PGC1alpha expression was found to attenuate alpha-synuclein-mediated mitochondrial dysfunction and subsequent neurotoxicity in vitro. Our data suggest that nuclear alpha-synuclein localization under conditions of oxidative stress may impact on mitochondrial function in part via the protein's capacity to act as a transcriptional modulator of PGC1alpha. This represents a novel role for alpha-synuclein as it relates to mitochondrial dysfunction in PD. | Aged, Animals, Cell Nucleus, Chromatin, Chromatin Immunoprecipitation, Electron Transport Complex I, Heat-Shock Proteins, Humans, Mice, Mice, Transgenic, Middle Aged, Mitochondria, Oxidative Stress, PC12 Cells, Parkinson Disease, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Promoter Regions, Genetic, Protein Binding, Rats, Transcription Factors, alpha-Synuclein | null |
22,705,957 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Constructing spatially separated multienzyme system through bioadhesion-assisted bio-inspired mineralization for efficient carbon dioxide conversion. | Shi Jiafu, Wang Xiaoli, Jiang Zhongyi, Liang Yanpeng, Zhu Yuanyuan, Zhang Chunhong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biocompatible Materials, Minerals, Multienzyme Complexes, NAD, Carbon Dioxide, Formaldehyde | IM | 22705957, S0960-8524(12)00723-7, 10.1016/j.biortech.2012.04.099 | A facile and green bioadhesion-assisted bio-inspired mineralization (BABM) approach is proposed to construct spatially separated multienzyme system for conversion of carbon dioxide to formaldehyde. Specifically, formate dehydrogenase is entrapped accompanying the formation of titania nanoparticles (NPs) through bio-inspired titanification. After in situ surface functionalization of NPs with oligodopa, formaldehyde dehydrogenase is immobilized on the surface of NPs through amine-catechol adduct reaction. Compared to co-immobilized and free multienzyme system, the spatially separated multienzyme system exhibits significantly enhanced formaldehyde yield, selectivity and initial specific activity. The influence of particle size on the enzyme activity reveals that the formaldehyde yield (80.9%, 52.9%, 46.4%), selectivity (92.7%, 86.6%, 85.1%) and initial specific activity (1.87, 1.31, 0.29 U mg(-1)) all decreased as the NPs particle size increased from 75, 175 to 375 nm. After storing for 20 days at 4 °C, this multienzyme system retains as high as 70% of its initial activity. | Adhesiveness, Biocompatible Materials, Carbon Dioxide, Formaldehyde, Green Chemistry Technology, Minerals, Multienzyme Complexes, NAD, Nanoparticles, Particle Size, Photoelectron Spectroscopy, Recycling | null |
22,705,956 | 2012-11-05 | 2018-12-20 | 1873-2976 | Bioresource technology | Bioreduction of methyl heteroaryl and aryl heteroaryl ketones in high enantiomeric excess with newly isolated fungal strains. | Pal Mohan, Srivastava Gautam, Moon Lomary S, Jolly Ravinder S | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Ketones, Pyridines, Pyrimidines, Reverse Transcriptase Inhibitors, Tubulin, 2-acetylpyridine, reverse transcriptase, Human immunodeficiency virus 1, HIV Reverse Transcriptase, PNU 142721 | IM | 22705956, S0960-8524(12)00775-4, 10.1016/j.biortech.2012.05.031 | Enantioenriched heteroaryl ethanols and aryl heteroarylmethanols are important intermediates and structural motifs in medicinal chemistry. Asymmetric biocatalytic reduction of corresponding ketones provides a straightforward approach for preparation of these compounds. Accordingly, three newly isolated fungal strains have been described, which produced the desired heteroaryl alcohols in high enantiomeric excess (ee). A broad substrate specificity was observed within these limited number of biocatalysts as demonstrated by preparation of a variety of heteroaryl alcohols, including (S)-5-(1-hydroxyethyl)furo[2,3-c]pyridine, a key intermediate for HIV-1 reverse transcriptase inhibitor, (S)-phenyl(pyridin-2-yl)methanol, an analgesic and (S,S)-2,6-bis(1-hydroxyethyl)pyridine, a chiral building block, mostly in >99% ee and 80-92% yield. Micro-morphologically, one of the isolate was found to be similar to Penicillium funiculosum. However, its β-tubulin sequence showed only 88% sequence identity with the known β-tubulin sequences of Penicillium. It may, therefore, represent a new species of Penicillium. The other biocatalysts were identified as Alternaria alternata and Talaromyces flavus. | Alternaria, Biocatalysis, Biodegradation, Environmental, Fungi, HIV Reverse Transcriptase, Ketones, Molecular Sequence Data, Oxidation-Reduction, Penicillium, Phylogeny, Pyridines, Pyrimidines, Reverse Transcriptase Inhibitors, Stereoisomerism, Substrate Specificity, Talaromyces, Tubulin | null |
22,705,955 | 2012-12-13 | 2024-06-10 | 1095-6867 | Hormones and behavior | Maternal gestational androgens are associated with decreased juvenile play in white-faced marmosets (Callithrix geoffroyi). | Birnie Andrew K, Hendricks Shelton E, Smith Adam S, Milam Ross, French Jeffrey A | eng | R01 HD042882 (NICHD NIH HHS, United States); R56 HD042882 (NICHD NIH HHS, United States); HD 42882 (NICHD NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | Androgens, Dihydrotestosterone, Testosterone | IM | 22705955, S0018-506X(12)00165-1, 10.1016/j.yhbeh.2012.05.014, PMC3586234, NIHMS387348, 8349280, 11958714, 7956464, 19646445, 15509387, 19446080, 19854190, 8372125, 11124885, 112249, 417178, 12954404, 31918517, 4847181, 10690883, 3126512, 15862182, 21328596, 5815161, 14529705, 14993060, 12606385, 3235069, 2286363, 16641996, 18157628, 8391165, 15579264, 31936970, 5166472, 19100266, 31973484, 6543252, 11467882, 678264, 32070083, 6514252, 7188648, 14432658, 1518002, 12414881, 19207815, 11786990, 31986813, 1801016, 111908 | Exposure to androgens during prenatal development shapes both physiological and behavioral developmental trajectories. Notably, in rhesus macaques, prenatal androgen exposure has been shown to increase rough-and-tumble play, a prominent behavioral feature in males during the juvenile period in primates. While macaques are an Old World, polygamous species with marked sexually dimorphic behavior, New World callitrichine primates (marmosets and tamarins) live in cooperative breeding groups and are considered to be socially monogamous and exhibit minimal sexual dimorphism in social play, which suggests that androgen may affect this species in different ways compared to macaques. In addition, we previously described considerable variation in maternal androgen production during gestation in marmosets. Here we tested the association between this variation and variation in offspring rough-and-tumble play patterns in both males and females. We measured testosterone and androstenedione levels in urine samples collected from pregnant marmoset mothers and then observed their offspring's play behavior as juveniles (5-10 months of age). In contrast to findings in rhesus macaques, hierarchical regression analyses showed that higher gestational testosterone levels, primarily in the second semester, were associated with decreased rough-and-tumble play in juveniles, and this relationship appears to be driven more so by males than females. We found no reliable associations between gestational androstenedione and juvenile play behavior. Our findings provide evidence to suggest that normative variation in levels of maternal androgen during gestation may influence developmental behavioral trajectories in marmosets in a way that contradicts previous findings in Old World primates. | Androgens, Animals, Animals, Newborn, Behavior, Animal, Callithrix, Dihydrotestosterone, Down-Regulation, Female, Male, Mothers, Play and Playthings, Pregnancy, Pregnancy, Animal, Prenatal Exposure Delayed Effects, Testosterone | null |
22,705,959 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | In situ catalytic pyrolysis of lignocellulose using alkali-modified amorphous silica alumina. | Zabeti M, Nguyen T S, Lefferts L, Heeres H J, Seshan K | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Alkalies, Aluminum Silicates, Biofuels, Carboxylic Acids, lignocellulose, Cesium, aluminosilicate, Lignin, Aluminum Oxide, Oxygen | IM | 22705959, S0960-8524(12)00778-X, 10.1016/j.biortech.2012.05.034 | Canadian pinewood was pyrolyzed at 450 °C in an Infrared oven and the pyrolysis vapors were converted by passing through a catalyst bed at 450 °C. The catalysts studied were amorphous silica alumina (ASA) containing alkali metal or alkaline earth metal species including Na, K, Cs, Mg and Ca. The catalysts effectiveness to reduce the bio-oil oxygen content, to enhance the bio-oil energy density and to change the liquid and gas product distribution were evaluated using different techniques including gravimetric analysis, elemental analysis, Karl-Fischer titration, GC/MS and micro-GC analysis. According to the results K/ASA found to be the most effective catalysts for conversion of hollocellulose (hemicellulose and cellulose)-derived vapors of pinewood while Cs/ASA catalyst was the most effective catalyst for conversion of lignin-derived vapors and production of hydrocarbons. | Alkalies, Aluminum Oxide, Aluminum Silicates, Biofuels, Biomass, Biotechnology, Canada, Carboxylic Acids, Catalysis, Cesium, Lignin, Molecular Weight, Oxygen, Pinus, Reproducibility of Results, Temperature, Thermodynamics, Volatilization | null |
22,705,951 | 2013-06-11 | 2022-03-17 | 1532-8651 | Regional anesthesia and pain medicine | Ultrasound-guided nerve block for inguinal hernia repair: a randomized, controlled, double-blind study. | Bærentzen Finn, Maschmann Christian, Jensen Kenneth, Belhage Bo, Hensler Margaret, Børglum Jens | eng | null | Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | Bupivacaine | IM | 22705951, 10.1097/AAP.0b013e31825a3c8a | Open inguinal hernia repair in adults is considered a minor surgical procedure but can be associated with significant pain. We aimed to evaluate acute postoperative pain management in male adults randomized to receive an ultrasound-guided ilioinguinal and iliohypogastric nerve block administered before surgery, in addition to a standard analgesic regimen. | Adult, Aged, Bupivacaine, Double-Blind Method, Female, Hernia, Inguinal, Herniorrhaphy, Humans, Male, Middle Aged, Nerve Block, Pain, Postoperative, Ultrasonography, Interventional | null |
22,705,960 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | Pyrolysis and combustion kinetics of date palm biomass using thermogravimetric analysis. | Sait Hani H, Hussain Ahmad, Salema Arshad Adam, Ani Farid Nasir | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Nitrogen | IM | 22705960, S0960-8524(12)00705-5, 10.1016/j.biortech.2012.04.081 | The present research work is probably the first attempt to focus on the kinetics of pyrolysis and combustion process for date palm biomass wastes like seed, leaf and leaf stem by using Thermogravimetric Analysis (TGA) technique. The physical properties of biomass wastes were also examined. Proximate and ultimate analysis of the date palm biomass was investigated. FT-IR analysis was conducted to determine possible chemical functional groups in the biomass. Results showed that date palm seed and leaf can be characterized as high calorific values and high volatile content biomass materials as compared to the leaf stem. Kinetic analysis of this biomass was also given a particular attention. It is concluded that these biomasses can become useful source of energy, chemicals and bio-char. | Air, Arecaceae, Biomass, Chemical Phenomena, Kinetics, Nitrogen, Plant Leaves, Plant Stems, Seeds, Temperature, Thermogravimetry | null |
22,705,958 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | High-yield production of meso-2,3-butanediol from cellodextrin by engineered E. coli biocatalysts. | Shin Hyun-Dong, Yoon San-Hwal, Wu Jianrong, Rutter Charles, Kim Seon-Won, Chen Rachel R | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Butylene Glycols, Dextrins, 2,3-butylene glycol, Cellulose, cellodextrin, Glucose | IM | 22705958, S0960-8524(12)00724-9, 10.1016/j.biortech.2012.04.100 | Escherichia coli has been engineered to produce a variety of biofuel and biorefinery products. However, it can only produce these products from simple sugars, requiring large amounts of enzymes to depolymerize cellulose into monomer sugars. Engineering E. coli to directly use cellodextrin, the partial hydrolysis product of cellulose, potentially could reduce the requirement of enzyme thereby the overall cost. Through a combination of gene deletion, introduction of a synthetic operon, and periplasmic expression of a Saccharophagus cellodextrinase, we engineered, for the first time, an E. coli biocatalyst capable of producing BDO from cellodextrin. The success of the engineering strategy is evidenced by the high BDO yield (>80%) from cellodextrin. We additionally demonstrate that the engineered biocatalyst can be advantageously used in a SSF process for BDO production from cellulose as the expression of cellodextrinase from a BDO producer augments the insufficient β-glucosidase activities in a commercial cellulase cocktail. | Biocatalysis, Butylene Glycols, Carbohydrate Metabolism, Cellulose, Dextrins, Escherichia coli, Extracellular Space, Fermentation, Glucose, Metabolic Engineering, Mutation | null |
22,705,961 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Effect of heat-alkaline treatment as a pretreatment method on volatile fatty acid production and protein degradation in excess sludge, pure proteins and pure cultures. | Tan Reasmey, Miyanaga Kazuhiko, Uy Davin, Tanji Yasunori | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Alkalies, Bacterial Proteins, Fatty Acids, Volatile, Sewage | IM | 22705961, S0960-8524(12)00808-5, 10.1016/j.biortech.2012.05.064 | This study investigated the effect of heat-alkaline treatment (HAT) at pH 11 and 60 °C on volatile fatty acid (VFA) production and protein degradation in excess sludge, soluble and insoluble proteins, and pure cultures. In addition, quantification of bacteria present in the sludge was also examined. Experimental results showed that following acid fermentation under pH 7 and 37 °C, HAT enhanced VFA production in excess sludge, albumin, and Gram-negative bacteria, but not in casein or Gram-positive bacteria. Protein solubility was therefore found not to be the main criteria for VFA production. In the protein analysis, it was shown that the outer membrane protein (OmpC) of Escherichia coli K12 was resistant to chemical and enzymatic hydrolysis. Gram staining revealed that Gram-negative bacteria were predominant in the activated sludge used in this study. In addition, the bacteria present in the activated sludge comprised only 10% of mixed liquor suspended solids (MLSS) by quantitative PCR. | Alkalies, Bacillus subtilis, Bacterial Proteins, Biotechnology, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fatty Acids, Volatile, Fermentation, Hot Temperature, Polymerase Chain Reaction, Proteolysis, Pseudomonas aeruginosa, Sewage, Staphylococcus aureus | null |
22,705,962 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Biodiversity and quantification of functional bacteria in completely autotrophic nitrogen-removal over nitrite (CANON) process. | Liu Tao, Li Dong, Zeng Huiping, Li Xiangkun, Zeng Taotao, Chang Xiaoyan, Cai Yan'an, Zhang Jie | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Nitrites, Ammonia, Nitrogen, Oxygen | IM | 22705962, S0960-8524(12)00780-8, 10.1016/j.biortech.2012.05.036 | The research was conducted to investigate the microbial diversity and population with the different concentration of NH(4)(+)-N in a biofilm reactor filled with volcanic filter for completely autotrophic nitrogen-removal over nitrite (CANON) process. The reactor had an excellent performance with the decreasing of NH(4)(+)-N concentration from 400 to 200 mg L(-1) while NH(4)(+)-N removal loading reduced at the NH(4)(+)-N concentration of 100 mg L(-1). Biodiversity analysis indicated that Nitrosomonas related aerobic ammonia oxidizing bacteria (AOB) and Planctomycetales-like anaerobic ammonia oxidizing (anammox) bacteria were dominant functional bacteria. Despite the different influent NH(4)(+)-N concentration, anammox bacteria had a low and stable biodiversity, which was not the same to AOB. With the concentration reduction of influent NH(4)(+)-N, the estimates of total bacteria population ranged between 2.29×10(11) and 1.44×10(12) copies mg(-1) total DNA, and the quantity of AOB decreased while anammox bacteria kept stable. The population of Nitrospira increased and little Nitrobacter was detected during the experiment. | Ammonia, Autotrophic Processes, Bacteria, Biodegradation, Environmental, Biodiversity, Bioreactors, Biotechnology, Denaturing Gradient Gel Electrophoresis, Hydrogen-Ion Concentration, Nitrites, Nitrogen, Oxidation-Reduction, Oxygen, Real-Time Polymerase Chain Reaction, Solubility | null |
22,705,963 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Comparing extraction buffers to identify optimal method to extract somatic coliphages from sewage sludges. | Murthi Poornima, Praveen Chandni, Jesudhasan Palmy R, Pillai Suresh D | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Buffers, Sewage | IM | 22705963, S0960-8524(12)00829-2, 10.1016/j.biortech.2012.05.076 | Somatic coliphages are present in high numbers in sewage sludge. Since they are conservative indicators of viruses during wastewater treatment processes, they are being used to evaluate the effectiveness of sludge treatment processes. However, efficient methods to extract them from sludge are lacking. The objective was to compare different virus extraction procedures and develop a method to extract coliphages from sewage sludge. Twelve different extraction buffers and procedures varying in composition, pH, and sonication were compared in their ability to recover indigenous phages from sludges. The 3% buffered beef extract (BBE) (pH 9.0), the 10% BBE (pH 9.0), and the 10% BBE (pH 7.0) with sonication were short-listed and their recovery efficiency was determined using coliphage-spiked samples. The highest recovery was 16% for the extraction that involved 10% BBE at pH 9.0. There is a need to develop methods to extract somatic phages from sludges for monitoring sludge treatment processes. | Anaerobiosis, Buffers, Coliphages, Hydrogen-Ion Concentration, Microbiological Techniques, Sewage, Sonication | null |
22,705,953 | 2013-06-11 | 2022-04-08 | 1532-8651 | Regional anesthesia and pain medicine | Incidence of local anesthetic systemic toxicity and postoperative neurologic symptoms associated with 12,668 ultrasound-guided nerve blocks: an analysis from a prospective clinical registry. | Sites Brian Daniel, Taenzer Andreas H, Herrick Michael D, Gilloon Constance, Antonakakis John, Richins Janeen, Beach Michael L | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anesthetics, Local | IM | 22705953, 10.1097/AAP.0b013e31825cb3d6 | There are varying reports on the incidence of major morbidity associated with peripheral regional anesthesia. Our objective was to contribute to the knowledge regarding the incidence of local anesthetic systemic toxicity and postoperative neurologic symptoms in the setting of ultrasound-guided peripheral regional anesthesia. | Adult, Aged, Anesthetics, Local, Cohort Studies, Female, Humans, Incidence, Male, Middle Aged, Nerve Block, Pain, Postoperative, Prospective Studies, Registries, Retrospective Studies, Ultrasonography, Interventional | null |
22,705,964 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Electric power generation by a submersible microbial fuel cell equipped with a membrane electrode assembly. | Min Booki, Poulsen Finn Willy, Thygesen Anders, Angelidaki Irini | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acetates, Membranes, Artificial | IM | 22705964, S0960-8524(12)00721-3, 10.1016/j.biortech.2012.04.097 | Membrane electrode assemblies (MEAs) were incorporated into the cathode chamber of a submersible microbial fuel cell (SMFC). A close contact of the electrodes could produce high power output from SMFC in which anode and cathode electrodes were connected in parallel. In polarization test, the maximum power density was 631 mW/m(2) at current density of 1772 mA/m(2) at 82 Ω. With 180-Ω external resistance, one set of the electrodes on the same side could generate more power density of 832±4 mW/m(2) with current generation of 1923±4 mA/m(2). The anode, inclusive a biofilm behaved ohmic, whereas a Tafel type behavior was observed for the oxygen reduction. The various impedance contributions from electrodes, electrolyte and membrane were analyzed and identified by electrochemical impedance spectroscopy. Air flow rate to the cathode chamber affected microbial voltage generation, and higher power generation was obtained at relatively low air flow less than 2 mL/min. | Acetates, Bioelectric Energy Sources, Dielectric Spectroscopy, Electric Power Supplies, Electricity, Electrodes, Membranes, Artificial, Rheology | null |
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