PMID
int64 275k
40M
| DateCompleted
stringdate 1965-11-14 00:00:00
2025-02-28 00:00:00
⌀ | DateRevised
stringdate 2000-12-18 00:00:00
2025-02-28 00:00:00
| ISSN
stringlengths 9
9
⌀ | JournalTitle
stringlengths 2
239
| ArticleTitle
stringlengths 1
1.99k
⌀ | Authors
stringlengths 3
61.6k
⌀ | Language
stringclasses 47
values | Grants
stringlengths 11
10.6k
⌀ | PublicationTypes
stringlengths 4
259
| Chemicals
stringlengths 3
1.33k
⌀ | CitationSubset
stringclasses 2
values | ArticleIds
stringlengths 6
44.3k
| Abstract
stringlengths 1
16.7k
⌀ | MeshTerms
stringlengths 3
1.18k
⌀ | Keywords
stringlengths 1
17.2k
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22,705,640 | 2013-02-05 | 2022-03-11 | 1873-3441 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V | Tacrolimus-loaded ethosomes: physicochemical characterization and in vivo evaluation. | Li Guiling, Fan Yating, Fan Chao, Li Xinru, Wang Xiaoning, Li Mei, Liu Yan | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Immunosuppressive Agents, Liposomes, Ethanol, Propylene Glycol, Cholesterol, Tacrolimus | IM | 22705640, S0939-6411(12)00178-6, 10.1016/j.ejpb.2012.05.011 | The purpose of this work was to prepare and characterize a novel ethosomal carrier for tacrolimus, an immunosuppressant treating atopic dermatitis (AD), and to investigate inhibition action upon allergic reactions of mice aiming at improving pharmacological effect for tacrolimus in that commercial tacrolimus ointment (Protopic®) with poor penetration capability exhibited weak impact on AD compared with common glucocorticoid. Results indicated that the ethosomes showed lower vesicle size and higher encapsulation efficiency (EE) as compared with traditional liposomes with cholesterol. In addition, the quantity of tacrolimus remaining in the epidermis at the end of the 24-h experiment was statistically significantly greater from the ethosomal delivery system than from commercial ointment (Protopic®) (p<0.01), suggesting the greater penetration ability to the deep strata of the skin for ethosomes. Interestingly, tacrolimus-loaded ethosomes with ethanol, in contrast to that with propylene glycol, showed relatively higher penetration activity except insignificant differences in EE and polydispersity index. Topical application of ethosomal tacrolimus displayed the lowest ear swelling in BALB/c mice model induced by repeated topical application of 2,4-dinitrofluorobenzene compared to traditional liposomes and commercial ointment and effectively impeded accumulation of mast cells in the ear of the mice, suggesting efficient suppression for the allergic reactions. In conclusion, the ethosomal tacrolimus delivery systems may be a promising candidate for topical delivery of tacrolimus in treatment of AD. | Administration, Cutaneous, Animals, Cholesterol, Dermatitis, Atopic, Disease Models, Animal, Drug Delivery Systems, Ethanol, Immunosuppressive Agents, Liposomes, Male, Mast Cells, Mice, Mice, Inbred BALB C, Particle Size, Propylene Glycol, Rats, Rats, Wistar, Skin Absorption, Tacrolimus, Time Factors | null |
22,705,639 | 2013-02-05 | 2013-11-21 | 1873-3441 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V | Uricase from Bacillus fastidious loaded in alkaline enzymosomes: enhanced biochemical and pharmacological characteristics in hypouricemic rats. | Tan Qunyou, Zhang Jingqing, Wang Na, Li Xiaoling, Xiong Huarong, Teng Yongzhen, He Dan, Wu Jianyong, Zhao Chunjing, Yin Huafeng, Zhang Liangke | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Liposomes, Uric Acid, Urate Oxidase | IM | 22705639, S0939-6411(12)00189-0, 10.1016/j.ejpb.2012.06.002 | The aim of this study was to assess the potential of a novel alkaline enzymosome to deliver uricase from Bacillus fastidious (UBF) and enhance its biochemical and pharmacological characteristics. The in vitro catalytic activity of the UBF loaded in the novel alkaline enzymosomes (ESUBFs) was almost 3.8 times that of free UBF at the optimum pH or 1.5 times that of free UBF at the physiological pH. Following intravenous (i.v.) administration (2000 mU/kg) in rats, ESUBFs provided significantly higher (22.5-fold) area under the plasma concentration (AUC) and longer (8.2-fold) circulation half-life (t(1/2)) compared with free UBF, respectively. Further, it took only 4.5h (or 1.1h) for ESUBFs to lower the plasma uric acid concentration from a high level to the normal level of rat (or human beings), compared with 7.6h (or 5.4h) for free UBF. Our results showed that ESUBFs could efficiently deliver UBF and favorably modify its biochemical and pharmacological characteristics by increasing the AUC, t(1/2), and catalytic activity. Therefore, ESUBFs might be a preferred alternative to cure hyperuricemia and gout. | Animals, Bacillus, Disease Models, Animal, Half-Life, Humans, Hydrogen-Ion Concentration, Hyperuricemia, Injections, Intravenous, Liposomes, Male, Rabbits, Rats, Rats, Sprague-Dawley, Time Factors, Urate Oxidase, Uric Acid | null |
22,705,641 | 2013-02-05 | 2022-12-07 | 1873-3441 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V | Folate-targeted supramolecular vesicular aggregates as a new frontier for effective anticancer treatment in in vivo model. | Paolino Donatella, Licciardi Mariano, Celia Christian, Giammona Gaetano, Fresta Massimo, Cavallaro Gennara | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antimetabolites, Antineoplastic, Drug Carriers, Hydrazines, Liposomes, Nylons, alpha,beta-polyasparthydrazide, Deoxycytidine, Folic Acid, Gemcitabine | IM | 22705641, S0939-6411(12)00188-9, 10.1016/j.ejpb.2012.06.001 | Supramolecular vesicular aggregates (SVAs), made up by self-assembling liposomes and polyasparthydrazide co-polymers conjugated to folic acid molecules were extensively investigated in this manuscript as potential active targeting formulation for anticancer drug delivery. Folate-targeted systems (FT-SVAs) were used to treat breast cancer and to further proof the potential in vivo administration of these systems for the therapeutic treatment for several aggressive solid tumors. The physicochemical and technological parameters of FT-SVAs are suitable for their potential in vivo administration. The chemotherapeutic activity of GEM-loaded FT-SVAs was increased during in vivo experiments. NOD-SCID mice bearing MCF-7 human xenograft is used as breast cancer model. The measurement of the volume and weight of tumor masses decreased when animal models are treated by using GEM-loaded FT-SVAs, compared to data obtained by using GEM-loaded mPEG-SUVs and the free form of GEM. An almost complete regression of the tumor (≈ 0.2 cm(3)) was observed in NOD-SCID mice bearing MCF-7 human xenografts treated by GEM-loaded FT-SVAs due to the noticeable improvement of GEM pharmacokinetic parameters provided by FT-SVAs with respect to native anticancer drug. The obtained data showed that supramolecular systems could represent an innovative drug delivery system by self-assembling liposomes and biocompatible polymers to be potentially used for anticancer treatment. | Animals, Antimetabolites, Antineoplastic, Breast Neoplasms, Deoxycytidine, Drug Carriers, Drug Delivery Systems, Female, Folic Acid, Humans, Hydrazines, Liposomes, MCF-7 Cells, Mice, Mice, Inbred NOD, Mice, SCID, Nylons, Xenograft Model Antitumor Assays, Gemcitabine | null |
22,705,642 | 2013-02-05 | 2012-09-11 | 1873-3441 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V | Cellular delivery of cationic lipid nanoparticle-based SMAD3 antisense oligonucleotides for the inhibition of collagen production in keloid fibroblasts. | Jin Su-Eon, Kim Chong-Kook, Kim Yang-Bae | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Collagen Type I, Lipids, Oligonucleotides, Antisense, SMAD3 protein, human, Smad3 Protein, Transforming Growth Factor beta | IM | 22705642, S0939-6411(12)00182-8, 10.1016/j.ejpb.2012.05.015 | SMAD3 is a key player in the TGFβ signaling pathway as a primary inducer of fibrosis. The inhibition of SMAD3 production is one strategy to alleviate fibrosis in keloid fibroblasts. In the present study, antisense oligonucleotides (ASOs) against SMAD3 were designed to specifically block the expression of SMAD3. The cationic lipid nanoparticles (cLNs) were formulated to enhance an intracellular activity of SMAD3 ASOs in keloid fibroblasts. This formulation was prepared using melt-homogenization method, composed of 3-[N-(N',N'-dimethylaminoethane)-carbamol] cholesterol (DC-Chol), dioleoylphosphatidylethanolamine (DOPE), Tween20, and trimyristin as a lipid core (1:1:1:1.3, w/w). The size and zeta potential of cLNs and cLN/ASO complexes were measured using light scattering. AFM was used to confirm the morphology and the size distribution of cLNs and cLN/ASO complexes. The prepared cLNs had a nano-scale sized spherical shape with highly positive charge, which were physically stable without aggregation during the storage. The cLN/SMAD3 ASO complexes were successfully generated and internalized onto keloid fibroblasts without toxicity. After the treatment with cLN/ASO complexes, SMAD3 was inhibited and collagen type I was also significantly suppressed in keloid fibroblasts. These results suggest that SMAD3 ASOs complexed with cLNs have a therapeutic potential to suppress collagen deposition in fibrotic diseases. Therefore, this strategy might be developed to lead to anti-fibrotic therapies. | Cells, Cultured, Collagen Type I, Drug Stability, Drug Storage, Fibroblasts, Fibrosis, Humans, Keloid, Light, Lipids, Nanoparticles, Oligonucleotides, Antisense, Particle Size, Scattering, Radiation, Signal Transduction, Smad3 Protein, Transforming Growth Factor beta | null |
22,705,643 | 2013-02-05 | 2013-11-21 | 1873-3441 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V | Transglycosylated rutin-specific non-surface-active nanostructure affects absorption enhancement of flurbiprofen. | Tozuka Yuichi, Higashi Kenjirou, Morita Takeshi, Nishikawa Masahiro, Uchiyama Hiromasa, Zhang Junying, Moribe Kunikazu, Nishikawa Keiko, Takeuchi Hirofumi, Yamamoto Keiji | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Excipients, Rutin, Flurbiprofen | IM | 22705643, S0939-6411(12)00172-5, 10.1016/j.ejpb.2012.05.005 | Transglycosylated rutin (Rutin-G), a newly developed transglycosylated food additive, was used as a novel excipient for improving the dissolution and absorption properties of flurbiprofen. No surface activity was found up to 100mg/mL of Rutin-G concentration. No cytotoxicity to Caco-2 cells was observed even at a high level of 100mg/mL Rutin-G solution. (1)H NMR study with concentration variation revealed that Rutin-G formed small aggregates in water, with the aggregation number of Rutin-G above the critical aggregation concentration of about 5.0mg/mL being 4. Structural analyses by small-angle X-ray scattering determined the aggregate to be several nanometers in maximum length. A solubility test of flurbiprofen in the presence of Rutin-G showed that the amount of dissolved flurbiprofen increased in proportion to the amount of Rutin-G loaded. This finding indicated a stoichiometric relationship between flurbiprofen and Rutin-G. The spray-dried particles of flurbiprofen/Rutin-G showed a significantly higher dissolution rate and greater absorption profile compared with the commercial flurbiprofen powder. Taken together, the results indicate the potential application of Rutin-G in the formation of a novel nanostructure of drug/transglycosylated material. | Animals, Caco-2 Cells, Dose-Response Relationship, Drug, Excipients, Flurbiprofen, Glycosylation, Humans, Magnetic Resonance Spectroscopy, Male, Nanostructures, Particle Size, Rats, Rats, Wistar, Rutin, Solubility | null |
22,705,644 | 2012-10-15 | 2016-11-25 | 1873-2968 | Biochemical pharmacology | CIL-102 binds to tubulin at colchicine binding site and triggers apoptosis in MCF-7 cells by inducing monopolar and multinucleated cells. | Gireesh K K, Rashid Aijaz, Chakraborti Soumyananda, Panda Dulal, Manna Tapas | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Tetrazolium Salts, Thiazoles, Tubulin, thiazolyl blue | IM | 22705644, S0006-2952(12)00406-6, 10.1016/j.bcp.2012.06.008 | A plant dictamine analog, 1-[4-(furo[2,3-b]quinolin-4-ylamino)phenyl]ethanone (CIL-102) has been shown to exert potent anti-tumor activity. In this study, we examined the mode of interaction of CIL-102 with tubulin and unraveled the cellular mechanism responsible for its anti-tumor activity. CIL-102 bound to tubulin at a single site with a dissociation constant ~0.4 μM. Isothermal titration calorimetry revealed that CIL-102-tubulin interaction is highly enthalpy driven and that the binding affords a large negative heat capacity change (ΔC(p) = -790 cal mol(-1) K(-1)) with an enthalpy-entropy compensation. An analysis of the modified Dixon plot suggested that CIL-102 competitively inhibited the binding of podophyllotoxin, a colchicine-binding site agent, to tubulin. Computational modeling indicated that CIL-102 binds exclusively at the β-subunit of tubulin and that CIL-102 and colchicine partially share their binding sites on tubulin. It bound to tubulin reversibly and the binding was estimated to be ~1000 times faster than that of colchicine. CIL-102 potently inhibited the proliferation of MCF-7 cells, induced monopolar spindle formation and multi-nucleation. At half-maximal inhibitory concentration, the spindle microtubules were visibly depolymerized and disorganized. CIL-102 reduced the inter-polar distances of bipolar mitotic cells indicating that it impaired microtubule-kinetochore attachments. CIL-102-treatment induced apoptosis in MCF-7 cells in association with increased nuclear accumulation of p53 and p21 suggesting that apoptosis is triggered through a p53-p21 dependent pathway. The results indicated that CIL-102 exerted anti-proliferative activity by disrupting microtubule functions through tubulin binding and provided important insights into the differential mode of tubulin binding by CIL-102 and colchicine. | Apoptosis, Binding Sites, Breast Neoplasms, Calorimetry, Cell Line, Tumor, Cell Proliferation, Humans, Kinetics, Spectrometry, Fluorescence, Tetrazolium Salts, Thermodynamics, Thiazoles, Tubulin | null |
22,705,645 | 2012-10-15 | 2021-01-03 | 1873-2968 | Biochemical pharmacology | Suppression of Akt/Foxp3-mediated miR-183 expression blocks Sp1-mediated ADAM17 expression and TNFα-mediated NFκB activation in piceatannol-treated human leukemia U937 cells. | Liu Wen-Hsin, Chang Long-Sen | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA Primers, FOXP3 protein, human, Forkhead Transcription Factors, MIRN183 microRNA, human, MicroRNAs, NF-kappa B, Sp1 Transcription Factor, Stilbenes, Tumor Necrosis Factor-alpha, 3,3',4,5'-tetrahydroxystilbene, Proto-Oncogene Proteins c-akt, ADAM Proteins, ADAM17 Protein, ADAM17 protein, human | IM | 22705645, S0006-2952(12)00405-4, 10.1016/j.bcp.2012.06.007 | To address the mechanism of piceatannol in inhibiting TNFα-mediated pathway, studies on piceatannol-treated human leukemia U937 cells were conducted. Piceatannol treatment reduced TNFα shedding and NFκB activation and decreased the release of soluble TNFα into the culture medium of U937 cells. Moreover, ADAM17 expression was down-regulated in piceatannol-treated cells. Over-expression of ADAM17 abrogated the ability of piceatannol to suppress TNFα-mediated NFκB activation. Piceatannol-evoked β-TrCP up-regulation promoted Sp1 degradation, thus reducing transcriptional level of ADAM17 gene in U937 cells. Piceatannol treatment induced p38 MAPK phosphorylation but inactivation of Akt and ERK. In contrast to p38 MAPK inhibitor or restoration of ERK activation, transfection of constitutive active Akt abolished the effect of piceatannol on β-TrCP, Sp1 and ADAM17 expression. Piceatannol-elicited down-regulation of miR-183 expression was found to cause β-TrCP up-regulation. Inactivation of Akt resulted in Foxp3 down-regulation and reduced miR-183 expression in piceatannol-treated cells. Knock-down of Foxp3 and chromatin immunoprecipitating revealed that Foxp3 genetically regulated transcription of miR-183 gene. Taken together, our data indicate that suppression of Akt/Foxp3-mediated miR-183 expression blocks Sp1-mediated ADAM17 expression in piceatannol-treated U937 cells. Consequently, piceatannol suppresses TNFα shedding, leading to inhibition of TNFα/NFκB pathway. | ADAM Proteins, ADAM17 Protein, Base Sequence, Chromatin Immunoprecipitation, DNA Primers, Down-Regulation, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Forkhead Transcription Factors, Humans, Leukemia, MicroRNAs, NF-kappa B, Proto-Oncogene Proteins c-akt, RNA Interference, Sp1 Transcription Factor, Stilbenes, Tumor Necrosis Factor-alpha, U937 Cells | null |
22,705,646 | 2012-10-15 | 2012-11-15 | 1873-2968 | Biochemical pharmacology | The vascular targeting agent Combretastatin-A4 directly induces autophagy in adenocarcinoma-derived colon cancer cells. | Greene Lisa M, O'Boyle Niamh M, Nolan Derek P, Meegan Mary J, Zisterer Daniela M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Stilbenes, Caspase 3, Caspase 7, fosbretabulin | IM | 22705646, S0006-2952(12)00403-0, 10.1016/j.bcp.2012.06.005 | Recent clinical data demonstrated that the vascular targeting agent Combretastatin-A4 phosphate (CA-4P) prolonged survival of patients with advanced anaplastic thyroid cancer without any adverse side effects. However, as a single agent CA-4 failed to reduce tumour growth in the murine CT-26 adenocarcinoma colon cancer model. Furthermore, the molecular mechanism of the innate resistance of HT-29 human adenocarcinoma cells to CA-4 is largely unknown. In this report, we demonstrate for the first time that prolonged exposure to CA-4 and an azetidinone cis-restricted analogue, CA-432 (chemical name; 4-(3-Hydroxy-4-methoxyphenyl)-3-phenyl-1-(3,4,5-trimethoxyphenyl)-azetidin-2-one) induced autophagy in adenocarcinoma-derived CT-26, Caco-2 and HT-29 cells but not in fibrosarcoma-derived HT-1080 cells. Autophagy is a fundamental self-catabolic process which can facilitate a prolonged cell survival in spite of adverse stress by generating energy via lysosomal degradation of cytoplasmic constituents. Autophagy was confirmed by acridine orange staining of vesicle formation, electron microscopy and increased expression of LC3-II. Combretastatin-induced autophagy was associated with a loss of mitochondrial membrane potential and elongation of the mitochondria. Furthermore, inhibition of autophagy by the vacuolar H(+)ATPase inhibitor Bafilomycin-A1 (BAF-A1) significantly enhanced CA-432 induced HT-29 cell death. Both CA-4 and its synthetic derivative, CA-432 induced the formation of large hyperdiploid cells in Caco-2 and CT-26 cells. The formation of these polyploid cells was significantly inhibited by autophagy inhibitor, BAF-A1. Results presented within demonstrate that autophagy is a novel response to combretastatin exposure and may be manipulated to enhance the therapeutic efficacy of this class of vascular targeting agents. | Adenocarcinoma, Autophagy, Blotting, Western, Caspase 3, Caspase 7, Cell Line, Tumor, Colonic Neoplasms, Flow Cytometry, Humans, Membrane Potentials, Microscopy, Electron, Mitochondria, Stilbenes | null |
22,705,647 | 2012-10-15 | 2012-07-30 | 1873-2968 | Biochemical pharmacology | MurD enzymes from different bacteria: evaluation of inhibitors. | Barreteau Hélène, Sosič Izidor, Turk Samo, Humljan Jan, Tomašić Tihomir, Zidar Nace, Hervé Mireille, Boniface Audrey, Peterlin-Mašič Lucija, Kikelj Danijel, Mengin-Lecreulx Dominique, Gobec Stanislav, Blanot Didier | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Enzyme Inhibitors, Peptide Synthases, UDP-N-acetylmuramoylalanine-D-glutamate ligase | IM | 22705647, S0006-2952(12)00404-2, 10.1016/j.bcp.2012.06.006 | D-Glutamic acid-adding enzyme (MurD ligase) catalyses the addition of D-glutamic acid to UDP-N-acetylmuramoyl-L-alanine, an essential cytoplasmic step in the pathway for bacterial cell-wall peptidoglycan synthesis. As such, it represents an important antibacterial drug-discovery target enzyme. Recently, several series of compounds have been synthesised and found to inhibit MurD from Escherichia coli, the best one having an IC(50) value of 8 μM. In the present work, we have tested 20 of these compounds against the MurD enzymes from Staphylococcus aureus, Streptococcus pneumoniae, Borrelia burgdorferi and Mycobacterium tuberculosis. Most of the E. coli MurD inhibitors appeared less efficient against the four other orthologues. This divergent result can be explained by the differences in amino acid sequences and topologies of the active sites of the MurD ligases studied. | Drug Evaluation, Preclinical, Enzyme Inhibitors, Escherichia coli, Inhibitory Concentration 50, Models, Molecular, Peptide Synthases, Polymerase Chain Reaction | null |
22,705,650 | 2012-11-05 | 2018-12-01 | 1941-7225 | American journal of hypertension | Retinopathy assessment in hypertension is still puzzling. | Triantafyllou Areti, Gavriilaki Eleni, Douma Stella | eng | null | Letter, Comment | null | IM | 22705650, ajh201246, 10.1038/ajh.2012.46 | null | Animals, Female, Humans, Hypertension, Male, Retinal Artery, Retinal Diseases | null |
22,705,648 | 2013-01-14 | 2022-03-18 | 1090-2147 | Brain and cognition | Factors affecting medial temporal lobe engagement for past and future episodic events: an ALE meta-analysis of neuroimaging studies. | Viard Armelle, Desgranges Béatrice, Eustache Francis, Piolino Pascale | eng | null | Journal Article, Meta-Analysis | null | IM | 22705648, S0278-2626(12)00085-1, 10.1016/j.bandc.2012.05.004 | Remembering the past and envisioning the future are at the core of one's sense of identity. Neuroimaging studies investigating the neural substrates underlying past and future episodic events have been growing in number. However, the experimental paradigms used to select and elicit episodic events vary greatly, leading to disparate results, especially with respect to the laterality and antero-posterior localization of hippocampal and adjacent medial temporal activations (i.e., parahippocampal, entorhinal and perirhinal cortices, amygdala). Although a central concern in today's literature, the issue of hippocampal and medial temporal lobe laterality and antero-posterior segregation in past and future episodic events has not yet been addressed extensively. Using the activation likelihood estimation (ALE) procedure (Turkeltaub, Eden, Jones, & Zeffiro, 2002), we performed a meta-analysis of hippocampal and adjacent medial temporal coordinates extracted from neuroimaging studies examining past remembering and future envisioning. We questioned whether methodological choices could influence the laterality of activations, namely (1) the type of cue used (generic vs. specific), (2) the type of task performed (recognition vs. recall/imagine), (3) the nature of the information retrieved (episodic vs. "strictly" episodic events) and (4) the age of participants. We consider "strictly" episodic events as events which are not only spatio-temporally unique and personal like episodic events, but are also associated with contextual and phenomenological details. These four factors were compared two-by-two, generating eight whole-brain statistical maps. Results indicate that (1) specific cues tend to activate more the right anterior hippocampus compared to the use of generic cues, (2) recall/imagine tasks tend to recruit more the left posterior parahippocampal gyrus compared to recognition tasks, (3) (re/pre)experiencing strictly episodic events tends to activate more the bilateral posterior hippocampus compared to episodic events and (4) older subjects tend to activate more the right anterior hippocampus compared to younger subjects. Importantly, our results stress that strictly episodic events triggered by specific cues elicit greater left posterior hippocampal activation than episodic events triggered by specific cues. These findings suggest that such basic methodological choices have an impact on the conclusions reached regarding past and future (re/pre)experiencing and their neural substrates. | Functional Neuroimaging, Humans, Memory, Episodic, Mental Recall, Temporal Lobe | null |
22,705,653 | 2012-12-11 | 2019-12-10 | 1470-2738 | Journal of epidemiology and community health | Structural stigma, sex work and HIV: contradictions and lessons learnt from a community-led structural intervention in southern India. | Biradavolu Monica Rao, Blankenship Kim M, Jena Asima, Dhungana Nimesh | eng | null | Journal Article | null | IM | 22705653, jech-2011-200508, 10.1136/jech-2011-200508 | Recent theorisation has pushed stigma research in new directions, arguing for a need to challenge the unequal power relations that impact groups most at risk for HIV-related stigma rather than locate stigma in the individual. Such a conceptualisation resonates with the growing emphasis on structural interventions for HIV prevention that attempt to alter the social context of risk. | Community Networks, Cross-Sectional Studies, Female, HIV Infections, Humans, India, Interviews as Topic, Organizations, Power, Psychological, Program Evaluation, Qualitative Research, Self Efficacy, Sex Work, Sex Workers, Sexually Transmitted Diseases, Social Stigma, Social Support, Socioeconomic Factors | null |
22,705,652 | 2013-01-23 | 2014-11-20 | 1532-2742 | The Journal of infection | Intra-articular injection of voriconazole for Fusarium solani arthritis after bone marrow transplantation. | Kawashima Nozomu, Yoshida Nao, Matsushita Naoya, Ito Masafumi, Matsumoto Kimikazu, Kato Koji | eng | null | Case Reports, Letter | Antifungal Agents, Pyrimidines, Triazoles, Voriconazole | IM | 22705652, S0163-4453(12)00158-2, 10.1016/j.jinf.2012.06.001 | null | Adolescent, Antifungal Agents, Arthritis, Bone Marrow Transplantation, Fusariosis, Fusarium, Histocytochemistry, Humans, Immunocompromised Host, Injections, Intra-Articular, Male, Microscopy, Pyrimidines, Skin, Triazoles, Voriconazole | null |
22,705,656 | 2012-12-03 | 2022-01-29 | 1470-2738 | Journal of epidemiology and community health | New perspectives on cardiovascular risk in individuals and in populations. | O'Flaherty Martin, Capewell Simon | eng | G0900847 (Medical Research Council, United Kingdom) | Editorial, Research Support, Non-U.S. Gov't | null | IM | 22705656, jech-2012-201409, 10.1136/jech-2012-201409 | null | Algorithms, Cardiovascular Diseases, Humans, Population Surveillance, Risk Assessment, Risk Factors | null |
22,705,655 | 2013-02-20 | 2012-11-07 | 1470-2738 | Journal of epidemiology and community health | The challenge of monitoring employment-related health inequalities. | Benach Joan, Puig-Barrachina Vanessa, Vives Alejandra, Tarafa Gemma, Muntaner Carles | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705655, jech-2012-201103, 10.1136/jech-2012-201103 | null | Employment, Health Status Disparities, Health Status Indicators, Humans, Occupational Health, Population Surveillance, Public Health, Public Policy, Socioeconomic Factors, Workplace | null |
22,705,654 | 2013-02-20 | 2016-11-25 | 1470-2738 | Journal of epidemiology and community health | Opening the black box of record linkage. | Harron Katie, Wade Angie, Muller-Pebody Berit, Goldstein Harvey, Gilbert Ruth | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705654, jech-2012-201376, 10.1136/jech-2012-201376 | null | Electronic Health Records, Humans, Medical Record Linkage, United Kingdom | null |
22,705,657 | 2013-04-15 | 2022-03-17 | 1470-2738 | Journal of epidemiology and community health | Distribution and determinants of risk of teenage motherhood in three British longitudinal studies: implications for targeted prevention interventions. | Kneale Dylan, Fletcher Adam, Wiggins Richard, Bonell Chris | eng | null | Journal Article | null | IM | 22705657, jech-2011-200867, 10.1136/jech-2011-200867 | In order to consider the potential contribution of universal versus targeted prevention interventions, the authors examined what is the distribution of established risk variables for teenage motherhood? from where in these distributions do births arise? and how does this distribution/determination of risk vary between studies? | Adolescent, Adolescent Behavior, Adult, Area Under Curve, England, Female, Humans, Logistic Models, Longitudinal Studies, Maternal Age, Middle Aged, Mothers, Pregnancy, Pregnancy in Adolescence, Prevalence, ROC Curve, Risk Factors, Socioeconomic Factors, Young Adult | null |
22,705,658 | 2013-04-15 | 2012-12-05 | 1470-2738 | Journal of epidemiology and community health | The changing relationship between income and mortality in Finland, 1988-2007. | Tarkiainen Lasse, Martikainen Pekka, Laaksonen Mikko | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705658, jech-2012-201097, 10.1136/jech-2012-201097 | Socioeconomic mortality differences have increased in many high-income countries in recent decades mainly because of slower mortality decline among the lower social groups. The aim of this study was to investigate whether the changing socio-demographic composition explains the increasing disparity in mortality by income and the stagnation of mortality in the lowest income group. | Adult, Data Collection, Family Characteristics, Female, Finland, Humans, Income, Life Expectancy, Logistic Models, Male, Middle Aged, Mortality, Registries, Sex Factors, Social Class, Socioeconomic Factors | null |
22,705,659 | 2012-08-22 | 2021-10-21 | 1471-0064 | Nature reviews. Genetics | Chromosome biology: Pairing up for the genetic exchange. | Stower Hannah | eng | null | Comment, Journal Article | null | null | 22705659, nrg3265, 10.1038/nrg3265, 22582262, 22654677, 22660327 | null | null | null |
22,705,670 | 2012-11-19 | 2015-11-19 | 1460-4744 | Chemical Society reviews | Elementary reactions of N atoms with hydrocarbons: first steps towards the formation of prebiotic N-containing molecules in planetary atmospheres. | Balucani Nadia | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Hydrocarbons, Nitrogen | IM | 22705670, 10.1039/c2cs35113g | Gas-phase reactions involving atomic nitrogen in the ground (4)S and first excited (2)D electronic states with simple hydrocarbons or hydrocarbon radicals lead to the formation of prebiotic N-containing organic molecules. These reactions are now active in the upper atmosphere of Titan (a massive moon of Saturn) and might have played an important role in nitrogen fixation in the primitive upper terrestrial atmosphere, assuming that it is similar to the present atmosphere of Titan. The products of these reactions (nitriles, imines and radicals) are the precursors of larger N-containing molecules, which form the dense haze aerosols that completely cover the moon of Saturn. If anything similar to Titan's haze has ever existed on our planet, it is reasonable to imagine that, once deposited on the surface of the oceans, further chemical evolution might have transformed these molecules into aminoacids and nucleobases, the molecular building blocks of living entities. The experimental techniques necessary to investigate these reactions in detail are presented and the main results reviewed. | Atmosphere, Evolution, Chemical, Extraterrestrial Environment, Hydrocarbons, Models, Molecular, Nitrogen, Origin of Life, Planets | null |
22,705,667 | 2012-08-22 | 2022-03-16 | 1471-0064 | Nature reviews. Genetics | Genomic approaches towards finding cis-regulatory modules in animals. | Hardison Ross C, Taylor James | eng | R01 DK065806 (NIDDK NIH HHS, United States); RC2 HG005573 (NHGRI NIH HHS, United States); U01 HG004695 (NHGRI NIH HHS, United States); U54 HG004695 (NHGRI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | null | IM | 22705667, nrg3242, 10.1038/nrg3242, PMC3541939, NIHMS429527, 21486936, 17984227, 9331366, 22153082, 17053094, 17846119, 20378774, 12529301, 10753117, 19305407, 19815776, 15340490, 17989251, 19563753, 20944595, 22072382, 8995271, 21152003, 17571346, 12097338, 12566409, 15630479, 17997594, 17086198, 12136103, 18436892, 17913488, 21187896, 2828161, 1090005, 22426492, 20887958, 21177974, 15297614, 15131651, 15716910, 19474294, 6277502, 2720468, 11752406, 19055709, 2704733, 19887574, 18243117, 19171877, 1406669, 17038566, 6308429, 22392219, 18165803, 21106903, 16953792, 2834649, 3199442, 20075146, 11125145, 8996792, 16606849, 6308460, 20729851, 14962988, 12837695, 19371447, 16269442, 20412780, 16645617, 9111322, 3296191, 17994087, 18818370, 17873874, 11997350, 20208536, 21179089, 17320500, 12107285, 8348617, 15357878, 17529977, 19853570, 19443739, 15649946, 6379641, 19295514, 10973062, 18250624, 20438361, 17277777, 22265404, 17446898, 18176564, 11253049, 19561604, 20363979, 21385855, 19029883, 16719718, 11825869, 21795386, 21441907, 8565831, 15153998, 17558387, 15706033, 15057822, 20299548, 21177976, 17540862, 21526222, 19317649, 12011446, 19212405, 20118932, 16606704, 17382889, 11805330, 1093168, 9571041, 17053093, 17567996, 6409417, 8464710, 15735639, 16469913, 20531367, 15494394, 20501695, 21307941, 19680443, 10458613, 20024537, 12676793, 19858363, 17994088, 19941826, 15545496, 19767611, 17241466, 15757364, 19636342, 18243105 | Differential gene expression is the fundamental mechanism underlying animal development and cell differentiation. However, it is a challenge to identify comprehensively and accurately the DNA sequences that are required to regulate gene expression: namely, cis-regulatory modules (CRMs). Three major features, either singly or in combination, are used to predict CRMs: clusters of transcription factor binding site motifs, non-coding DNA that is under evolutionary constraint and biochemical marks associated with CRMs, such as histone modifications and protein occupancy. The validation rates for predictions indicate that identifying diagnostic biochemical marks is the most reliable method, and understanding is enhanced by the analysis of motifs and conservation patterns within those predicted CRMs. | Animals, Chromatin Immunoprecipitation, Epigenesis, Genetic, Gene Expression Regulation, Genomics, High-Throughput Screening Assays, Regulatory Elements, Transcriptional | null |
22,705,671 | 2012-10-23 | 2012-06-18 | 1536-3708 | Annals of plastic surgery | When N = 1: the role of the case report. | Shale Christopher M, Weber Robert A | eng | null | Editorial | null | IM | 22705671, 10.1097/SAP.0b013e31825df77f, 00000637-201207000-00001 | null | Editorial Policies, Evidence-Based Medicine, Periodicals as Topic, Sample Size | null |
22,705,668 | 2012-08-22 | 2024-06-10 | 1471-0064 | Nature reviews. Genetics | Human aneuploidy: mechanisms and new insights into an age-old problem. | Nagaoka So I, Hassold Terry J, Hunt Patricia A | eng | T32 GM008336 (NIGMS NIH HHS, United States); R01 HD021341 (NICHD NIH HHS, United States); R56 ES013527 (NIEHS NIH HHS, United States); R01 ES013527 (NIEHS NIH HHS, United States); R37 HD021341 (NICHD NIH HHS, United States); ES013527 (NIEHS NIH HHS, United States); HD21341 (NICHD NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Endocrine Disruptors | IM | 22705668, nrg3245, 10.1038/nrg3245, PMC3551553, NIHMS423265, 12646133, 10385520, 11576729, 19763179, 22033395, 22366948, 20971813, 21493685, 12077403, 9285774, 21795248, 11106739, 21813122, 22513370, 17696610, 19249208, 20817533, 18096426, 9159442, 20971462, 15240554, 1740312, 17509208, 20817531, 21059909, 18239089, 8944019, 13662687, 21093264, 16322543, 19696121, 6241455, 1867285, 18083607, 22095483, 14504407, 17312021, 18369452, 17222059, 20627979, 20941357, 17204525, 10856933, 9412457, 17329371, 22002499, 18663141, 21282942, 15657431, 8222760, 18664475, 20833308, 20956811, 20634189, 21957193, 8908177, 4230650, 7887421, 17901045, 16530707, 11934988, 20817534, 1954032, 20484246, 11207209, 8432196, 10819752, 21617258, 19409550, 14668819, 21908463, 87796, 15485352, 17292877, 17945219, 13642857, 14525526, 21093783, 16546077, 16824959, 7573048, 12522562, 9499419, 21743440, 22552228, 19805400, 18252055, 11134360, 21122836, 1279409, 2401885, 11283700, 19188923, 2369835, 881666, 8943533, 18093867, 21782164, 17618286, 8643515, 21487227, 15471934, 3906118, 19300349, 12052900, 8046027, 11014823, 1999340, 16258540, 20044539, 22513372, 1437215, 19770126, 12730498, 21730149, 21497085, 11980735, 9512249, 21122835, 17425408, 15580272, 17891138, 19965510, 8944020, 15879462, 19865085, 9914385, 17426725, 13678590, 12676084 | Trisomic and monosomic (aneuploid) embryos account for at least 10% of human pregnancies and, for women nearing the end of their reproductive lifespan, the incidence may exceed 50%. The errors that lead to aneuploidy almost always occur in the oocyte but, despite intensive investigation, the underlying molecular basis has remained elusive. Recent studies of humans and model organisms have shed new light on the complexity of meiotic defects, providing evidence that the age-related increase in errors in the human female is not attributable to a single factor but to an interplay between unique features of oogenesis and a host of endogenous and exogenous factors. | Age Factors, Aneuploidy, Chromosome Segregation, Endocrine Disruptors, Female, Humans, M Phase Cell Cycle Checkpoints, Meiosis, Oogenesis, Reproductive Techniques, Assisted, Sex Factors | null |
22,705,672 | 2012-10-23 | 2018-12-01 | 1536-3708 | Annals of plastic surgery | Re: A controlled clinical trial with pirfenidone in the treatment of pathological skin scarring caused by burns in pediatric patients. | Freshwater M Felix | eng | null | Letter, Comment | Anti-Inflammatory Agents, Non-Steroidal, Pyridones | IM | 22705672, 10.1097/SAP.0b013e31824ba4fd, 00000637-201207000-00025 | null | Anti-Inflammatory Agents, Non-Steroidal, Burns, Cicatrix, Hypertrophic, Compression Bandages, Female, Humans, Male, Pyridones | null |
22,705,669 | 2012-08-22 | 2021-10-21 | 1471-0064 | Nature reviews. Genetics | Comparative studies of gene expression and the evolution of gene regulation. | Romero Irene Gallego, Ruvinsky Ilya, Gilad Yoav | eng | P50 GM081892 (NIGMS NIH HHS, United States); GM077959 (NIGMS NIH HHS, United States); GM084996 (NIGMS NIH HHS, United States); R01 GM077959 (NIGMS NIH HHS, United States); R01 GM084996 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Review | Transcription Factors | IM | 22705669, nrg3229, 10.1038/nrg3229, PMC4034676, NIHMS575834, 20625544, 16525476, 22005986, 5713805, 20378774, 22046143, 19252487, 28556213, 17492956, 19478187, 20941394, 21908772, 20106546, 20617162, 19164747, 11923494, 15085123, 19506578, 22022286, 21593416, 22102826, 17304246, 16143608, 20351773, 15809270, 18682548, 18787134, 9533962, 19307593, 20007865, 20194736, 1090005, 19023414, 22398555, 11951044, 21119629, 11275748, 16921347, 21390129, 22162950, 17943131, 19411596, 15138501, 20461072, 16494531, 28563044, 12192408, 22012392, 19307592, 12548287, 18986212, 20369021, 10539941, 19490899, 19720867, 21721942, 18616572, 5160087, 16121257, 17542651, 19937974, 18550803, 16980977, 20836039, 16567645, 19015660, 22069375, 17530920, 18772437, 21383968, 21811398, 20009012, 22307276, 21655084, 21350176, 5789433, 16281035, 18597885, 12610534, 19818800, 17277777, 21946353, 22207615, 21924514, 21914852, 19407207, 18278046, 21321133, 21441907, 16687733, 19451592, 16094311, 21974994, 15101252, 21925323, 20007773, 20299548, 15549674, 14661024, 21896781, 21946354, 16915236, 16642024, 21778360, 16179473, 18172436, 16806568, 20501695, 16825572, 8536981, 21122937, 824182, 22291600, 15792233, 18687876, 21850043, 18614008, 15757364 | The hypothesis that differences in gene regulation have an important role in speciation and adaptation is more than 40 years old. With the advent of new sequencing technologies, we are able to characterize and study gene expression levels and associated regulatory mechanisms in a large number of individuals and species at an unprecedented resolution and scale. We have thus gained new insights into the evolutionary pressures that shape gene expression levels and have developed an appreciation for the relative importance of evolutionary changes in different regulatory genetic and epigenetic mechanisms. The current challenge is to link gene regulatory changes to adaptive evolution of complex phenotypes. Here we mainly focus on comparative studies in primates and how they are complemented by studies in model organisms. | Adaptation, Biological, Animals, Biological Evolution, Epigenesis, Genetic, Gene Expression Regulation, Genetic Speciation, Models, Genetic, Physiology, Comparative, Primates, Selection, Genetic, Species Specificity, Transcription Factors | null |
22,705,674 | 2013-04-18 | 2016-11-25 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Electron paramagnetic resonance, optical absorption and Raman spectral studies on a pyrite/chalcopyrite mineral. | Udayabhaskar Reddy G, Seshamaheswaramma K, Nakamura Yoshinobu, Lakshmi Reddy S, Frost Ray L, Endo Tamio | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Minerals, Powders, Sulfides, chalcopyrite, pyrite, Copper, Iron | IM | 22705674, S1386-1425(12)00446-5, 10.1016/j.saa.2012.04.093 | Pyrite and chalcopyrite mineral samples from Mangampet barite mine, Kadapa, Andhra Pradesh, India are used in the present study. XRD data indicate that the pyrite mineral has a face centered cubic lattice structure with lattice constant 5.4179 Å. Also it possesses an average particle size of 91.9 nm. An EPR study on the powdered samples confirms the presence of iron in pyrite and iron and Mn(II) in chalcopyrite. The optical absorption spectrum of chalcopyrite indicates presence of copper which is in a distorted octahedral environment. NIR results confirm the presence of water fundamentals and Raman spectrum reveals the presence of water and sulfate ions. | Absorption, Copper, Electron Spin Resonance Spectroscopy, Iron, Minerals, Optical Phenomena, Powders, Spectroscopy, Near-Infrared, Spectrum Analysis, Raman, Sulfides, Temperature, X-Ray Diffraction | null |
22,705,675 | 2013-04-18 | 2012-09-24 | 1873-3557 | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | The investigation of the binding behavior between ethyl maltol and human serum albumin by multi-spectroscopic methods and molecular docking. | Yue Yuanyuan, Liu Jianming, Yao Meihuan, Yao Xiaojun, Fan Jing, Ji Hanxuan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Ligands, Pyrones, Serum Albumin, ethyl maltol | IM | 22705675, S1386-1425(12)00497-0, 10.1016/j.saa.2012.05.041 | This paper was designed to investigate the interaction of ethyl maltol with human serum albumin (HSA) under physiological condition by fluorescence, synchronous fluorescence, three-dimensional fluorescence, Fourier transformation infrared spectra, and molecular docking method. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of HSA by ethyl maltol was static quenching mechanism. The binding constants of ethyl maltol-HSA complexes were observed to be 2.59, 1.88, 1.54, 1.13×10(4) M(-1) at 289, 296, 303 and 310 K, respectively. The thermodynamic parameters, ΔH(0) and ΔS(0) were calculated to be -28.61 kJ mol(-1) and -14.59 J mol(-1) K(-1). Energy transfer from tryptophan to ethyl maltol occurred by a FRET mechanism, and the donor-acceptor distance (3.04 nm) had been determined according to Förster's theory. Molecular docking studies revealed that ethyl maltol situated within subdomain IIA (site I) of HSA. Fluorescence displacement experiments also proved the binding sites between ethyl maltol and HSA. | Energy Transfer, Humans, Ligands, Molecular Docking Simulation, Protein Binding, Pyrones, Serum Albumin, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Spectrum Analysis, Thermodynamics | null |
22,705,677 | 2013-03-12 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | The only exoribonuclease present in Haloferax volcanii has an unique response to temperature changes. | Matos Rute G, López-Viñas Eduardo, Goméz-Puertas Paulino, Arraiano Cecília M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | RNA, Double-Stranded, Exoribonucleases, ribonuclease R | IM | 22705677, S0304-4165(12)00176-6, 10.1016/j.bbagen.2012.06.009 | Little is known regarding mRNA degradation mechanisms in archaea. In some of these single-cell organisms the existence of a complex of exoribonucleases called the exosome has been demonstrated. However, in halophilic archaea the RNase R homologue is essential since it is the only enzyme described with exoribonucleolytic activity. | Amino Acid Sequence, Catalysis, Enzyme Activation, Exoribonucleases, Haloferax volcanii, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, RNA Stability, RNA, Double-Stranded, Sequence Homology, Amino Acid, Temperature | null |
22,705,679 | 2012-08-14 | 2012-06-18 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Current topics on neoadjuvant chemotherapy for resectable esophageal cancer]. | Mori Kazuhiko, Yamagata Yukinori, Seto Yasuyuki | jpn | null | English Abstract, Journal Article | null | IM | 22705679 | Chemoradiotherapy is considered a standard neoadjuvant therapy for advanced esophageal cancer in western countries. However, in Japan, experts usually do not indicate preoperative radiotherapy for resectable diseases, considering the excellent local disease control with surgery alone and postoperative morbidity related to radiation. The benefit of neoadjuvant chemotherapy using the FP regimen has been demonstrated by the latest trial named JCOG9907, and preoperative use of the FP regimen is now increasing in Japan. Although the FP regimen in a neoadjuvant setting may become a standard care, the optimal stage selection for neoadjuvant chemotherapy is still controversial. In addition, the DCF regimen, known as a stronger and more effective regimen for advanced disease, is reported as a more attractive regimen, also in neoadjuvant settings. | Esophageal Neoplasms, Humans, Meta-Analysis as Topic, Neoadjuvant Therapy, Neoplasm Staging | null |
22,705,678 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [The mechanisms of resistance to EGFR-TKIs and challenges to overcome resistance in EGFR mutant non-small cell lung cancer]. | Yamaoka Toshimitsu, Ohmori Tohru | jpn | null | Journal Article, Review | Protein Kinase Inhibitors, ErbB Receptors | IM | 22705678 | Somatic activating mutations in the epidermal growth factor receptor (EGFR) gene were first identified in 2004 from tumor tissues of non-small cell lung cancer (NSCLC) patients and pulmonary adenocarcinoma cell lines. Although pulmonary adenocarcinoma patients harboring EGFR mutations have increased sensitivity to EGFR tyrosine kinase inhibitors (TKIs), gefitinib and erlotinib, the primary and acquired resistant cases remain major clinical problems. Therapeutic strategies for such oncogene-driven carcinomas were intensively investigated at both the clinical and preclinical levels. In this review, we focused on one particular molecularly-defined subset of NSCLC that harbors activating mutations in the EGFR gene. We summarized the rational dissection of the mechanisms of drug sensitivity and resistance to EGFR-TKIs, and the promising molecular-centric strategies for further improving the outcomes of NSCLC patients with EGFR activating mutations. | Carcinoma, Non-Small-Cell Lung, Drug Resistance, Neoplasm, ErbB Receptors, Humans, Lung Neoplasms, Mutation, Protein Kinase Inhibitors | null |
22,705,680 | 2012-08-14 | 2012-06-18 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Neoadjuvant chemotherapy for gastric cancer--evidence from the world and future strategy in Japan]. | Yoshikawa Takaki, Aoyama Toru, Hayashi Tsutomu, Kuwabara Hiroshi, Mikayama Yo, Ogata Takashi, Cho Haruhiko, Tsuburaya Akira | jpn | null | English Abstract, Journal Article | Antineoplastic Agents | IM | 22705680 | Adjuvant chemotherapy is aimed at eradicating residual micro-metastatic tumor cells existing at the distant site outside of the surgical field. From evidence gathered from around the world, several courses of intensive neoadjuvant chemotherapy are promising even in Japan, where D2 surgery is the standard for local control. The Japan Clinical Oncology Group (JCOG) plays a central role in the development of neoadjuvant chemotherapy in Japan. Two JCOG Phase II trials clarified that neoadjuvant chemotherapy was effective for bulky nodal disease. A JCOG Phase III is now on-going to confirm the efficacy of neoadjuvant chemotherapy of S-1+CDDP for cancer of the schirrhous type. In another group, two randomized Phase II trials are also ongoing to compare different regimens and courses of neoadjuvant chemotherapy. These studies will set the direction of neoadjuvant chemotherapy development in the future. | Antineoplastic Agents, Clinical Trials as Topic, Humans, Neoadjuvant Therapy, Stomach Neoplasms | null |
22,705,682 | 2012-08-14 | 2014-11-20 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [The development of neoadjuvant chemotherapy in breast cancer]. | Taguchi Tetsuya | jpn | null | English Abstract, Journal Article, Review | Antineoplastic Agents, ERBB2 protein, human, Receptor, ErbB-2 | IM | 22705682 | In the beginning, neoadjuvant chemotherapy (NAC) is part of the multidisciplinary treatment of locally advanced breast cancer. Many clinical trials in the past have developed the usefulness of anthracycline containing NAC for operable breast cancer. The majority of trials have proven survival rates equivalent to adjuvant chemotherapy with increased breast conservation rates, and have also shown that a pathological complete response (pCR) after NAC is associated with improved survival. Taxanes have been introduced into clinical trials of NAC with increased pCR rates. However, there was no significant difference in overall survival among the regimens which added taxanes to neoadjuvant anthracycline. In this paper, the problems of NAC up to the present and the development of new regimens are discussed. We also discuss the new concept of NAC which might revolutionize what we know about NAC. | Antineoplastic Agents, Breast Neoplasms, Female, Humans, Neoadjuvant Therapy, Receptor, ErbB-2 | null |
22,705,681 | 2012-08-14 | 2012-06-18 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Neoadjuvant chemotherapy for colorectal cancer]. | Sato Takeo, Naito Masanori, Ikeda Atsushi, Ogura Naoto, Miura Hirohisa, Tsutsui Atsuko, Nakamura Takatoshi, Watanabe Masahiko | jpn | null | English Abstract, Journal Article | Antineoplastic Agents | IM | 22705681 | Surgery continues to play an important role in the curative treatment of gastrointestinal cancer. Recently, considerable progress has been made in chemotherapy and radiotherapy. In particular, chemotherapy with FOLFIRI and FOLFOX has prolonged survival in patients with colorectal cancer. Molecular-targeted agents have also enhanced the effectiveness of chemotherapy. However, radical resection offers the potential for a cure and is unsurpassed by any other treatments. Nonetheless, further improvement in survival is unlikely to be achieved by surgery alone. Studying how treatment regimens highly effective against unresectable or recurrent colorectal cancer can be adapted to patients with resectable disease is thus an important issue. | Antineoplastic Agents, Chemoradiotherapy, Colorectal Neoplasms, Humans, Neoadjuvant Therapy, Neoplasm Metastasis | null |
22,705,683 | 2012-08-14 | 2012-06-18 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Neoadjuvant chemotherapy for ovarian cancer]. | Onda Takashi | jpn | null | English Abstract, Journal Article, Review | Antineoplastic Agents | IM | 22705683 | The current standard treatment for advanced ovarian cancer is primary debulking surgery (PDS) followed by postsurgical chemotherapy. We can expect better prognosis in cases where optimal debulking (residual diseases<1 cm) can be achieved. Neoadjuvant chemotherapy (NAC) has been recognized as an alternative treatment to primary surgical debulking for patients with poor performance status or apparently unresectable bulky tumors. Retrospective analyses and non-randomized comparative studies revealed that overall survival was comparable between patients treated with NAC followed by interval debulking surgery (IDS) and those treated with PDS, though the former group had more advanced disease and poorer performance status. Two reports of meta-analyses of these studies revealed that the NAC setting treatment does not compromise the treatment outcome of the patients with advanced ovarian cancer. Until now, at least four phase III studies comparing NAC setting treatment with standard treatment for advanced müllerian cancer have been conducted. The results of the first study conducted by the European Organization for Research and Treatment of Cancer (EORTC) were published in 2010. They revealed a comparative outcome of NAC setting treatment with standard treatment (median survival 30 M vs 29 M) with less common surgery-related adverse effects. NAC setting treatment is now expected to become a standard treatment or one of the effective treatment options for advanced ovarian cancer in cases when other phase III studies reproduce similar results. | Antineoplastic Agents, Clinical Trials, Phase III as Topic, Female, Humans, Meta-Analysis as Topic, Neoadjuvant Therapy, Ovarian Neoplasms | null |
22,705,685 | 2012-08-14 | 2016-11-25 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Protocol for administration of trastuzumab based on cardiac function]. | Okada Yoshinobu, Kaneko Koji, Kanbayashi Chizuko, Sato Nobuaki | jpn | null | Journal Article | Antibodies, Monoclonal, Humanized, Trastuzumab | IM | 22705685 | We analyzed 7 patients with breast cancer who suffered from trastuzumab-associated cardiac dysfunction. Left ventricular ejection fraction (EF) was calculated by M-mode echocardiography. We propose a protocol for the administration of trastuzumab based on EF in the clinical courses of the 7 patients and the literatures concernig trastuzumab. If the EF is ≥60%, an echocardiography is performed every 3 months during trastuzumab therapy. If the EF is ≥53% and ≤60%, an echocardiography is performed every 2 months. If the EF is ≤53%, an echocardiography is performed more frequently. If the EF decreases by ≥10% from its previous level, an echocardiography is performed after 3 weeks. Also, trastuzumab is withheld when the EF falls to ≤45%. When the EF falls to ≤40%, the standard treatment for congestive heart failure is initiated. An echocardiography is performed every 2 weeks after trastuzumab is withheld. Trastuzumab therapy can be resumed if the EF improves to ≥50%. | Adult, Aged, 80 and over, Antibodies, Monoclonal, Humanized, Breast Neoplasms, Female, Humans, Middle Aged, Trastuzumab, Ultrasonography, Ventricular Dysfunction, Left | null |
22,705,676 | 2013-03-14 | 2021-10-21 | 0006-3002 | Biochimica et biophysica acta | Bioreactors to influence stem cell fate: augmentation of mesenchymal stem cell signaling pathways via dynamic culture systems. | Yeatts Andrew B, Choquette Daniel T, Fisher John P | eng | R01 AR061460 (NIAMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Culture Media | IM | 22705676, S0304-4165(12)00174-2, 10.1016/j.bbagen.2012.06.007, PMC3461086, NIHMS386547, 10734116, 20345129, 17925003, 18841496, 20929287, 15486964, 10609520, 15709706, 17303404, 22029448, 18314188, 18366089, 19686060, 19308769, 12124795, 11783438, 22247071, 15604272, 17318529, 11641401, 16982090, 19280635, 19226211, 19820164, 10319868, 17185377, 22422570, 22097912, 15132997, 15254968, 11255176, 21810479, 18220880, 16579680, 12831733, 21953870, 15121013, 20682444, 12893825, 16331674, 18086670, 21992078, 17969035, 12471242, 14657343, 19198002, 12857422, 15573378, 21396709, 17925579, 14517865, 19401766, 21608139, 15189819, 15869916, 19426107, 19834955, 9069255, 15133024, 15250049, 21902622, 21360689, 14606520, 16479590, 16023419, 14964727, 11278290, 15579652, 14960578, 19232075, 18431785, 17620285, 17521616, 15694410, 17276151, 21067807, 16041628, 19197334, 22056755, 21450070, 19272346, 11319758, 21495897, 9121483, 11744620, 9732876, 15786511, 20504065, 19708077, 18384159, 16864785, 10794673, 15165456, 18601588, 21895493, 15686620, 11336300, 5580029, 16905540, 10102814, 17477447, 21726624, 17081517, 17316462, 21171934, 19283726, 20626350, 17720811, 1126089, 20952688, 17081601, 16565087, 11792318, 17916040, 15623506, 22528808, 12242339, 15895382, 20799909, 17518749, 15657936, 12454419, 15094297, 17516855, 16948169, 19349579, 19432813, 21809383, 22224439, 18352827, 10753864, 20528664, 16133930, 21931630, 16080711, 11057897, 11790549, 20932947, 11854297, 19698058, 12743126, 20556515, 18246193, 18855520, 18204048, 16267109, 16125223, 18491951, 18620487, 20557888 | Mesenchymal stem cells (MSCs) are a promising cell source for bone and cartilage tissue engineering as they can be easily isolated from the body and differentiated into osteoblasts and chondrocytes. A cell based tissue engineering strategy using MSCs often involves the culture of these cells on three-dimensional scaffolds; however the size of these scaffolds and the cell population they can support can be restricted in traditional static culture. Thus dynamic culture in bioreactor systems provides a promising means to culture and differentiate MSCs in vitro. | Bioreactors, Cell Lineage, Culture Media, Stem Cells | null |
22,705,684 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Gastrojejunostomy followed by chemotherapy with S-1 in unresectable gastric cancer with pyloric stenosis]. | Matsushima Yuko, Kawabata Ryohei, Imamura Hiroshi, Kishimoto Tomono, Hachino Yoshimi, Yasui Yukako, Fujino Misako, Fujii Chika, Fukunaga Mutsumi, Ohzato Hiroki, Furukawa Hiroshi | jpn | null | English Abstract, Journal Article | Antimetabolites, Antineoplastic, Drug Combinations, S 1 (combination), Tegafur, Oxonic Acid | IM | 22705684 | We investigated the efficacy of gastrojejunostomy followed by S-1-based chemotherapy for unresectable gastric cancer with pyloric stenosis. We performed gastrojejunostomy and S-1-based chemotherapy in 14 unresectable gastric cancer patients with gastric outlet obstructions between April 2006 and June 2010. Although there were two complications after surgery, no treatment-related deaths were observed. The response rate of the S-1-based chemotherapy was 41.7%, and the median survival after surgery was 12.3 months. All patients were tolerating a regular diet and a significant improvement in oral intake lasted for at least 6 months. In conclusion, gastrojejunostomy followed by chemotherapy with S-1 appears to be an effective treatment modality for unresectable gastric cancer with pyloric stenosis. It enables us to practice S-1-based standard chemotherapy for advanced gastric cancer and improve the quality of life of patients. | Adult, Aged, Aged, 80 and over, Antimetabolites, Antineoplastic, Combined Modality Therapy, Drug Combinations, Female, Gastric Bypass, Humans, Male, Middle Aged, Oxonic Acid, Pyloric Stenosis, Stomach Neoplasms, Tegafur | null |
22,705,686 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Concurrent chemoradiotherapy with either CDDP or CDGP, plus 5-FU for squamous cell carcinoma of the oropharynx and hypopharynx]. | Tomita Masahiko, Matsuyama Hiroshi, Yamazaki Keisuke, Sato Katsuro, Takahashi Sugata | jpn | null | English Abstract, Journal Article | Organoplatinum Compounds, nedaplatin, Cisplatin, Fluorouracil | IM | 22705686 | Clinical outcomes of 53 patients with oropharyngeal and hypopharyngeal squamous cell carcinoma, treated from January, 2000 to December, 2008 by concurrent chemoradiotherapy of either CDDP or CDGP, plus 5-FU were investigated. Patients were treated with either CDDP (70 mg/m2) or CDGP (100 mg/m2) on day 1 of the chemotherapy regime, with 5-FU (700 mg/m2/day) as a continuous infusion for 5 days. Each regimen was administered as two courses in the first and final weeks of radiotherapy. Radiotherapy was administered at a daily dose of 2 Gy for five days a week, with patients receiving a total of 70 Gy by the end of seven weeks. The primary cancer was located in the oropharynx and hypopharynx in 21 and 32 patients, respectively. Twenty-six patients (49.1%) had stage IVA disease and 10 patients (18.9%) had overall stage I to II disease. Acute adverse events such as pharyngeal mucositis and leucopenia occurred in 49.1% and 43.4% of patients, respectively, and second round chemotherapy was not commenced in 56.6% of patients (n=30) due to significant adverse events. Mean weight loss following treatment was 4.1 kg. After a median follow-up of 30.0 months, 3-year overall survival was 53.0% for advanced carcinoma of the oropharynx and hypopharynx. Five-year overall survival was 46.4%. Patients receiving two courses of chemotherapy had an improved 5-year survival compared to patients receiving one course (67.0% vs 32.8%). Results indicate a significant benefit from two courses of chemotherapy. As such, minimizing the incidence of adverse effects and thereby reducing treatment discontinuation will likely improve overall treatment outcomes. | Adult, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols, Carcinoma, Squamous Cell, Chemoradiotherapy, Cisplatin, Female, Fluorouracil, Humans, Hypopharyngeal Neoplasms, Male, Middle Aged, Neoplasm Staging, Organoplatinum Compounds, Oropharyngeal Neoplasms, Stomatitis, Weight Loss | null |
22,705,687 | 2012-08-14 | 2022-04-08 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Adverse events during pemetrexed administration caused by concomitant nonsteroid anti-inflammatory therapy]. | Sakata Yasuhiko, Iwamoto Yasuo, Inata Junya, Matsumoto Shunji, Miyamori Shinichi, Nakashima Kazutoshi, Sakamoto Shinjiro, Kanehara Masashi, Kitaguchi Souichi, Hiraki Kouichi | jpn | null | English Abstract, Journal Article | Anti-Inflammatory Agents, Non-Steroidal, Antimetabolites, Antineoplastic, Glutamates, Pemetrexed, Guanine, Creatinine | IM | 22705687 | Pemetrexed, a folate metabolic antagonist, is considered to be effective against plural mesotheliomas, non-small cell lung cancer, and especially for non-squamous cell cancer. However, it has been reported to have adverse interactions with nonsteroid anti-inflammatory drugs(NSAIDs). In the present study, we compared the incidence of adverse events between patients receiving pemetrexed therapy with and without concomitant NSAID administration. No significant difference in the incidence of hematotoxic events of Grade 3 or worse was observed. As for the incidence of non-hematotoxic events, the increase in the amount of creatinine, namely a severe adverse effect of Grade 2 or more, was significantly higher in the combined therapy group (p=0.018). No other significant differences were noted for other adverse events. A creatinine increase to Grade 2 or greater developed significantly earlier in the combined group(median value, 12.7 courses; p=0.0063). Our results suggest that renal dysfunction may easily develop as a result of continued pemetrexed administration combined with NSAID therapy. Therefore, it is necessary to take precautions against adverse side effects such as renal dysfunction when combining pemetrexed with NSAID therapy, by conducting periodic examinations. | Aged, Anti-Inflammatory Agents, Non-Steroidal, Antimetabolites, Antineoplastic, Creatinine, Female, Glutamates, Guanine, Humans, Kidney Diseases, Male, Middle Aged, Neoplasms, Pemetrexed, Retrospective Studies | null |
22,705,688 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Efficacy of aprepitant for quality of life of outpatients receiving moderately emetogenic chemotherapy]. | Mochinaga Sakiko, Sumi Shintaro, Yakabe Tomomi, Aragane Naoko, Noshiro Hirokazu, Kimura Shinya, Fujito Hiroshi | jpn | null | Journal Article | Antiemetics, Antineoplastic Agents, Morpholines, Aprepitant | IM | 22705688 | We examined the antiemetic effect and impact of aprepitant on the quality of life (QOL) of outpatients receiving moderately emetogenic chemotherapy (MEC). Data were compared between patients who received aprepitant (aprepitant group, n=30, treated May to September 2010) and those who did not (control group, n=14, treated February to April 2010). Controls received antiemetic treatment with a serotonin receptor antagonist and dexamethasone on Day 1. The aprepitant group received oral aprepitant (125 mg) concomitantly with these drugs on Day 1, and aprepitant (80 mg) on Days 2 and 3. The percentages of subjects without vomiting and nausea during the overall phase (0-96 h), acute phase (0-24 h), and delayed phase (24-96 h), and without loss of appetite during the entire period and delayed phase, were significantly higher in the aprepitant group. A QOL evaluation using the Functional Living Index-Emesis (FLIE) questionnaire showed a significantly higher percentage of subjects without the impact of nausea and vomiting disturbing their daily lives during the overall phase, and a significant decrease in QOL disturbance in the aprepitant group. Our results suggest that nausea, vomiting, loss of appetite, and QOL disturbance occur in many outpatients undergoing MEC, and that concomitant therapy with 3 drugs including aprepitant can improve symptoms and QOL of these patients. | Antiemetics, Antineoplastic Agents, Appetite, Aprepitant, Female, Humans, Male, Middle Aged, Morpholines, Nausea, Neoplasms, Outpatients, Quality of Life, Vomiting | null |
22,705,689 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [The importance of bone management for cancer bone disease--positioning of zoledronic acid for multiple myeloma, genitor-urinary cancers (renal cancer and prostate cancer), lung cancer and breast cancer]. | Tokuhashi Yasuaki, Abe Masahiro, Shinohara Nobuo, Takeda Koji, Takahashi Shunji | jpn | null | Journal Article | Bone Density Conservation Agents, Diphosphonates, Imidazoles, Zoledronic Acid | IM | 22705689 | With the advances in early diagnosis and treatment of cancer, prognosis of cancer patients has been improved significantly. Therefore improvement of QOL for cancer patients has become an important issue. Prevention of skeletal related event is one of the major factors to improve QOL in patients with bone metastasis. Since the clinical introduction of zoledronic acid, management of bone lesions has been improved and decreased SRE significantly. The specialists from four disease area which is multiple myeloma, urological cancer, lung cancer and breast cancer have discussed the benefits (and risks?) of the zoledronic acid with the current status of bone management in each cancer. | Bone Density Conservation Agents, Bone Neoplasms, Diphosphonates, Humans, Imidazoles, Quality of Life, Zoledronic Acid | null |
22,705,690 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of thyroid medullary carcinoma with multiple painful bone metastases successfully treated with strontium-89 chloride]. | Oshiro Chiya, Kamigaki Shunji, Arai Takashi, Nakamura Yukio, Fukunaga Mutsumi, Ichida Wakako, Ikeda Hiroshi | jpn | null | Case Reports, English Abstract, Journal Article | strontium chloride, Strontium | IM | 22705690 | A 70-year-old man was diagnosed as thyroid medullary carcinoma with multiple bone metastases. He underwent total thyroidectomy and cervical lymph node dissection. After one year, the pain from his bone lesions was becoming severe. To relieve the pain, he was administered opioids and external-beam radiation therapy. However, he continued to have substantial multiple bone pain. We used combination therapy of strontium-89 chloride for the treatment of widespread multiple bone pain and external-beam radiation therapy for localized pain. That combination therapy was effective and improved the QOL of the patient. We used strontium-89 chloride four times within one year, and no serious side effects occurred during therapy. Our thoroughly investigated case suggests that strontium-89 therapy is one of the effective and safe therapies for patients with painful bone metastases of thyroid medullary carcinoma. | Aged, Back Pain, Bone Neoplasms, Carcinoma, Neuroendocrine, Humans, Male, Neoplasm Staging, Palliative Care, Quality of Life, Strontium, Thyroid Neoplasms | null |
22,705,691 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of drug-induced pulmonary injury showing organizing pneumonia pattern due to S-1]. | Nakata Hiroaki, Shinano Hideki, Kuraya Daisuke, Fujioka Yasunori | jpn | null | Case Reports, English Abstract, Journal Article | Antimetabolites, Antineoplastic, Drug Combinations, S 1 (combination), Tegafur, Oxonic Acid | IM | 22705691 | Reported here is the case of a 76-year-old male with gastric cancer. Distal gastrectomy was performed after his admission to our hospital. Histopathologically, the cancer was determined to be in the advanced stage. Combination chemotherapy with CDDP and S-1 was administered for 6 courses, after which S-1 was used alone. Chest X-ray and CT showed multiple dispersed lesions in the lung. Further examination by bronchoscope was performed. Histopathological examination of a biopsy specimen revealed the lesion to be organizing pneumonia. A drug-induced lymphocyte stimulation test (DLST) for S-1 proved to be positive. Discontinuation of S-1 administration led to natural improvement of the pneumonia. These results suggest that S-1 had induced the organizing pneumonia. | Aged, Antimetabolites, Antineoplastic, Biopsy, Drug Combinations, Humans, Lung Injury, Male, Oxonic Acid, Pneumonia, Stomach Neoplasms, Tegafur, Tomography, X-Ray Computed | null |
22,705,692 | 2012-08-14 | 2025-01-03 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of hepatic metastases from uveal melanoma treated effectively by transcatheter arterial chemoembolization with cisplatin and degradable starch microspheres]. | Sakane Makoto, Sato Yozo, Yamaura Hidekazu, Kato Mina, Kanamoto Takaaki, Tomozawa Yuki, Inaba Yoshitaka, Muro Kei | jpn | null | Case Reports, English Abstract, Journal Article | Antineoplastic Agents, Starch, Cisplatin | IM | 22705692 | A 37-year-old male presented with hepatic metastases from uveal melanoma after the enucleation of an affected eye. Hepatic metastases were thought to be the critical factors determining prognosis, so transcatheter arterial chemoembolization (TACE) was performed for local control of the hepatic metastases. The first TACE with cisplatin (CDDP) and gelatin sponge (GS) did not have much success because fine feeding arteries to the main hepatic tumor on the caudate lobe branched out from the hepatic artery, and GS particles were not distributed in the tumor vessels. We used degradable starch microspheres (DSM) as finer obstructing material for the next treatment, and hepatic metastases were treated effectively with repeated CDDP/DSM-TACE. | Adult, Antineoplastic Agents, Chemoembolization, Therapeutic, Cisplatin, Fatal Outcome, Hepatic Artery, Humans, Liver Neoplasms, Male, Melanoma, Microspheres, Neovascularization, Pathologic, Starch, Tomography, X-Ray Computed, Uveal Neoplasms, Uveal Melanoma | null |
22,705,693 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of advanced hepatocellular carcinoma with portal vein invasion successfully treated by sorafenib]. | Endo Yoshikatsu, Miyamoto Manabu, Koujima Takeshi, Watanabe Yusuke, Kawai Takashi, Toda Keisuke, Nobuhisa Tetsuji, Watanabe Takanori, Matsumoto Yusuke, Watanabe Naoki, Kai Kyohei, Sato Shizou | jpn | null | Case Reports, Journal Article | Antineoplastic Agents, Benzenesulfonates, Phenylurea Compounds, Pyridines, Niacinamide, Sorafenib | IM | 22705693 | A 73-year-old man was followed up for HCV-associated chronic hepatitis and hepatocellular carcinoma (HCC), developed in segment 8 of the liver. Radiofrequency ablation (P-RFA) was used to treat the tumor in June 2004. Afterwards, the patient underwent repetitive transcatheter arterial chemoembolization (TACE) against recurrent tumors 5 times. An abdominal computed tomogram (CT) showed an infiltrative mass in the left liver with tumor thrombus invading into the umbilical portion. Transarterial infusion (TAI) therapy of cisplatin (CDDP) was performed 2 times, in January and June of 2010. The size of the main tumor was decreased according to CT, and tumor marker levels such as AFP and PIVKA-II also decreased, but tumor thrombus of the portal vein developed into the main trunk (Vp4). We started therapy with sorafenib in July, 2010. Two months later, an abdominal CT revelaed further reduction of the main tumor and a shrunken tumor thrombus of the portal vein back to the left lobe. The therapeutic effect of sorafenib against HCC with tumor thrombus of the portal vein continued for 9 months. | Aged, Antineoplastic Agents, Benzenesulfonates, Carcinoma, Hepatocellular, Hepatitis C, Chronic, Humans, Liver Neoplasms, Male, Neoplasm Invasiveness, Niacinamide, Phenylurea Compounds, Portal Vein, Pyridines, Sorafenib | null |
22,705,694 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of response to panitumumab as third-line chemotherapy for multiple liver metastases and portal venal tumor embolus of rectal cancer]. | Kimura Youhei, Goi Takanori, Sawai Katsuji, Iida Atsushi, Katayama Kanji, Yamaguchi Akio | jpn | null | Case Reports, Journal Article | Antibodies, Monoclonal, Antineoplastic Agents, Organoplatinum Compounds, Panitumumab, Leucovorin, Fluorouracil | IM | 22705694 | A 64-year-old man who underwent rectal amputation for rectal cancer was diagnosed with multiple liver metastases and tumor embolus in the portal vein 6 months after operation. Though the patient underwent chemotherapy, mFOLFOX6, and bevacizumab+FOLFIRI, liver metastases were diagnosed as progressive disease (PD). After panitumumab+FOLFIRI was administered for three months as third-line chemotherapy, the tumor embolus completely disappeared, and liver metastases became cytoreductive on CT. The patient was judged to have achieved a partial response (PR). This case indicated that panitumumab was effective as third-line chemotherapy for unresectable recurrent rectal cancer. | Antibodies, Monoclonal, Antineoplastic Agents, Antineoplastic Combined Chemotherapy Protocols, Embolism, Fluorouracil, Humans, Leucovorin, Liver Neoplasms, Male, Middle Aged, Neoplasm Invasiveness, Neoplasm Staging, Organoplatinum Compounds, Panitumumab, Portal Vein, Rectal Neoplasms, Salvage Therapy | null |
22,705,695 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case report of advanced gastric cancer with peritoneal dissemination effectively treated by combination chemotherapy of S-1 and docetaxel]. | Enomoto Koji, Takayama Tomoyoshi, Matsumoto Sohei, Wakatsuki Kohei, Tanaka Tetsuya, Migita Kazuhiro, Ito Masahiro, Nakajima Yoshiyuki | jpn | null | Case Reports, Journal Article | Drug Combinations, Taxoids, S 1 (combination), Tegafur, Docetaxel, Oxonic Acid | IM | 22705695 | The present patient was a 69-year-old male diagnosed as gastric cancer with peritoneal dissemination by staging laparoscopy. He was treated with chemotherapy using S-1 (120 mg/body/day) and docetaxel (70 mg/body/day 1) administered for 2 weeks, followed by one drug-free week in three-week courses. After 4 courses of treatment, the primary tumor regressed, but only slightly. Because of an adverse event, we continued with a lower dose. After 4 more courses of treatment, the primary tumor and dissemination were undetectable on abdominal CT scan but were endoscopically detected. The patient has been followed on an outpatient basis without surgical treatment for 2 years. | Aged, Antineoplastic Combined Chemotherapy Protocols, Docetaxel, Drug Combinations, Humans, Male, Neoplasm Staging, Oxonic Acid, Peritoneal Neoplasms, Stomach Neoplasms, Taxoids, Tegafur, Tomography, X-Ray Computed | null |
22,705,696 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of advanced gastric cancer with multiple liver metastases that achieved long-term survival on monotherapy of S-1]. | Masuishi Toshiki, Sakai Yoshinori, Soma Tomoko, Nagayama Kazunori, Kusano Fumihiko, Tazawa Junichi | jpn | null | Case Reports, English Abstract, Journal Article | Antimetabolites, Antineoplastic, Drug Combinations, S 1 (combination), Tegafur, Oxonic Acid | IM | 22705696 | A 74-year-old woman was referred to us for patterns of liver metastases found in abdominal ultrasonography. Upper gastrointestinal endoscopy revealed advanced gastric cancer, and the subsequent abdominal CT examination confirmed multiple liver metastases. After the first regimen, a combination of 5-FU and CDDP failed due to adverse events, so monotherapy of S-1 was introduced. An abdominal CT examination in the 21st month showed disappearance of liver metastases. She was judged to have a complete response and continued with this treatment. She survived five years and eight months on monotherapy of S-1 before she died of bronchiectasis. | Aged, Antimetabolites, Antineoplastic, Bronchiectasis, Drug Combinations, Fatal Outcome, Female, Humans, Liver Neoplasms, Oxonic Acid, Stomach Neoplasms, Tegafur, Tomography, X-Ray Computed | null |
22,705,697 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Long-term progression-free survival after reduction surgery and postoperative low-dose imatinib administration for multiple liver metastases of duodenal gastrointestinal stromal tumor]. | Nishitai Ryuta, Manaka Dai, Uehara Masahiro, Hamasu Shinya, Konishi Sayuri, Sakamoto Katsunori, Yoshino Kenji, Kanto Satoshi, Yokoyama Daiju, Kobayashi Aki, Jinzai Yuki, Yasuhara Yumiko | jpn | null | Case Reports, English Abstract, Journal Article | Antineoplastic Agents, Benzamides, Piperazines, Pyrimidines, Imatinib Mesylate | IM | 22705697 | A fifty-six year-old woman visited our institute, suffering from lower abdominal pain. A tumor was palpable in the pelvic cavity, having the diameter of 9.7 cm, as measured by transvaginal ultrasonography (US). Computed tomography and magnetic resonance imaging (MRI) revealed a high contrast-enhancement and the central necrosis of the tumor. Surgical resection was performed, and the tumor was found to have originated in the duodenum. Immunohistochemistry confirmed positive KIT, and the mitotic index was 4 per 50 high power field, so that the final diagnosis was a gastrointestinal stromal tumor of intermediate risk. After two years of observation, multiple liver metastases were found. Hepatectomy was performed as a volume reduction surgery, leaving three small lesions in the remnant liver. Imatinib administration was initiated at 400 mg a day two weeks after the surgery, but was interrupted two weeks later because of severe anorexia and a body weight gain of 7 kg due to the increased ascites and edema. Imatinib was resumed at 200 mg/day after a one-month interval. She has been enjoying relapse-free survival for 8 years since the recurrence was diagnosed. Although neither reduction surgery nor dose reduction of imatinib below 300 mg/day is recommended, there may be a possibility that a smaller tumor might be controlled by a lower dose of imatinib. | Antineoplastic Agents, Benzamides, Combined Modality Therapy, Disease-Free Survival, Duodenal Neoplasms, Female, Gastrointestinal Stromal Tumors, Humans, Imatinib Mesylate, Liver Neoplasms, Magnetic Resonance Imaging, Middle Aged, Piperazines, Pyrimidines, Tomography, X-Ray Computed | null |
22,705,698 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [FOLFIRI with bevacizumab chemotherapy for a patient with recurrence of rectal cancer under haemodialysis for chronic renal failure]. | Hoshino Hiroki, Ishii Yoshiyuki, Hasegawa Hirotoshi, Endo Takashi, Ochiai Hiroki, Hoshino Yoshinori, Matsunaga Atsushi, Shigeta Kohei, Seo Yuki, Hoshino Go, Kitagawa Yuko | jpn | null | Case Reports, English Abstract, Journal Article, Review | Antibodies, Monoclonal, Humanized, Bevacizumab, Leucovorin, Fluorouracil, Camptothecin | IM | 22705698 | As pharmacokinetics in patients undergoing haemodialysis is different from patients with normal renal function, it remains unclear whether chemotherapy can be performed safely for patients with haemodialysis as well as those who have normal renal function. Here, we report a case with recurrence of rectal cancer who received FOLFIRI with bevacizumab chemotherapy under haemodialysis, and obtained good tumor control. A 47-year-old woman had undergone haemodialysis for 10 years due to chronic renal failure. At 45 years of age, she received abdominoperineal resection due to rectal cancer (pStage II). Four months after the surgery, liver metastasis was found, for which partial resection of the liver and adjuvant chemotherapy [UFT (400 mg/body)/UZEL (75 mg/body)] were performed. Eighteen months after the liver resection, multiple lung metastases were found. Therefore, intensive chemotherapy using FOLFIRI (CPT-11: 90 mg/m2) with bevacizumab (2.5 mg/m2) was performed. Severe neutropenia (grade 3, 4), but not non-hematologic adverse events such as diarrhea and bevacizumab-specific adverse events, was observed. As she did not recover easily from neutropenia in spite of treatment with G-CSF, a dose reduction of the FOLFIRI regimen was gradually performed. Although chemotherapy was conducted approximately monthly, the tumor response reflected a stable disease 8 months after 8 courses of chemotherapy. We suggest that it is important to investigate the pharmacokinetics of toxic agents such as CPT-11, (SN38) for dose modification, and for the safe and continuous chemotherapy of patients receiving haemodialysis. | Antibodies, Monoclonal, Humanized, Antineoplastic Combined Chemotherapy Protocols, Bevacizumab, Camptothecin, Female, Fluorouracil, Humans, Kidney Failure, Chronic, Leucovorin, Liver Neoplasms, Lung Neoplasms, Middle Aged, Rectal Neoplasms, Recurrence | null |
22,705,699 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of pathologically by complete response in advanced sigmoid colon cancer with multiple metastases of lung and Liver, left hydronephrosis after chemotherapy including bevacizumab/FOLFOX6]. | Kubo Hidefumi, Nishiyama Mitsuo, Tada Kosuke, Miyahara Makoto, Hasegawa Hiroyasu, Yamashita Yoshimi | jpn | null | Case Reports, English Abstract, Journal Article | Antibodies, Monoclonal, Humanized, Organoplatinum Compounds, Bevacizumab, Leucovorin, Fluorouracil | IM | 22705699 | A 61-year-old complaining of anorexia and general fatigue was admitted to our hospital for further examination. She was diagnosed as advanced sigmoid colon cancer with multiple metastases of lung, liver, and left hydronephrosis. Since curative surgery was not deemed possible, we started chemotherapy with bevacizumab/FOLFOX6 (bi-weekly drip infusion). After the 6th course, colonoscopy revealed a significant tumor reduction and changes to the scar tissues. CT did not reveal a complete disappearance, but found some reductions in metastases of lung and liver. Sigmoidectomy and lymph node resection (D1) were performed. We did not disappeared any dissemination and the histological diagnosis revealed a complete disappearance of cancer cells in the main tumor. She was discharged 13 days after surgery, following chemotherapy which included bevacizumab and XELOX. The chemotherapy using bevacizumab/FOLFOX6 is a candidate for the standard treatment strategy for inoperable advanced colon cancer. Herein we report this rare case with a review of the literature. | Antibodies, Monoclonal, Humanized, Antineoplastic Combined Chemotherapy Protocols, Bevacizumab, Female, Fluorouracil, Humans, Hydronephrosis, Leucovorin, Liver Neoplasms, Lung Neoplasms, Middle Aged, Organoplatinum Compounds, Sigmoid Neoplasms, Tomography, X-Ray Computed | null |
22,705,700 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of advanced colon cancer with peritoneal dissemination effectively treated with modified FOLFOX6 chemotherapy]. | Tanaka Kazumi, Hirai Keitaro, Ogawa Hiroomi, Toya Hiroyuki, Totsuka Osamu, Yoshinari Daisuke, Sunose Yutaka, Takeyoshi Izumi | jpn | null | Case Reports, English Abstract, Journal Article | Organoplatinum Compounds, Leucovorin, Fluorouracil | IM | 22705700 | A 50-year-old woman was diagnosed with ascending colon cancer with bilateral ovarian metastases, carcinomatous peritonitis, and carcinomatous pleurisy. Nine courses of mFOLFOX6 treatment resulted in the disappearance of her ascites and pleural effusion and a marked decrease in her serum CEA and CA19-9 levels. Additionally, the primary tumor and ovarian metastases became smaller. Therefore, a right hemicolectomy with D3 lymph node dissection, total hysterectomy, and bilateral salpingo-oophorectomy were performed. Postoperatively, we changed the chemotherapy from mFOLFOX6 to bevacizumab+FOLFIRI because the patient had an allergic reaction to oxaliplatin, and we suspected lung metastasis. Because the lung metastasis grew after ten courses of bevacizumab+FOLFIRI, we changed to cetuximab+FOLFIRI. Unfortunately, 28 months after her diagnosis, the patient died of carcinomatous pleurisy. | Antineoplastic Combined Chemotherapy Protocols, Colonic Neoplasms, Fatal Outcome, Female, Fluorouracil, Humans, Leucovorin, Middle Aged, Neoplasm Grading, Organoplatinum Compounds, Ovarian Neoplasms, Peritoneal Neoplasms | null |
22,705,702 | 2012-08-14 | 2020-12-09 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case report of pathologically complete response of locally advanced rectal cancer after neoadjuvant chemoradiotherapy with XELOX and bevacizumab]. | Miyazawa Tomonori, Ebe Kazuyu, Fujita Nobuhiro, Koide Norihiko, Honma Kenji, Ikarashi Toshihiko | jpn | null | Case Reports, Journal Article | Antibodies, Monoclonal, Humanized, Oxaloacetates, Deoxycytidine, Bevacizumab, Capecitabine, Fluorouracil | IM | 22705702 | A 70-year-old man was admitted to our hospital for constipation. A clinical examination showed locally advanced rectal cancer with possible invasion to the prostate gland and pelvic wall. After performing colostomy, he underwent neoadjuvant radiation therapy (40 Gy) and six courses of a XELOX and bevacizumab regimen. A subsequent examination demonstrated significant reduction of the tumor, so we performed super low anterior resection and colo-anal anastomosis. Pathological examination revealed no residual cancer cells and showed pathological CR. Neoadjuvant chemoradiotherapy with XELOX and bevacizumab were useful for down staging and function-preserving surgery in patients with locally advanced rectal cancer. | Aged, Antibodies, Monoclonal, Humanized, Antineoplastic Combined Chemotherapy Protocols, Bevacizumab, Capecitabine, Deoxycytidine, Fluorouracil, Humans, Male, Neoadjuvant Therapy, Oxaloacetates, Rectal Neoplasms, Tomography, X-Ray Computed | null |
22,705,701 | 2012-08-14 | 2013-11-21 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [Complete disappearance of pulmonary metastases in a case of rectal cancer treated with oral tegafur-uracil (UFT) and leucovorin (LV)]. | Takada Atsushi, Kawahara Masaki, Kobayashi Hiroaki, Takino Youko, Kodama Takashi, Sugishita Takeo, Ohno Yasuo, Oka Teruaki, Tsukamoto Fumihito | jpn | null | Case Reports, English Abstract, Journal Article | Tegafur, Uracil, Leucovorin | IM | 22705701 | We report a case of complete remission after treatment with tegafur-uracil (UFT)/Leucovorin (LV) therapy for pulumonary metastasis of rectal cancer. A 56-year-old male was admitted to our hospital with a diagnosis of rectal cancer (Ra, type2). Chest CT on admission demonstrated bilateral lung metastases (rt S2 and lt S4). After anterior resection of the primary tumor, oral UFT/LV was administered (UFT 400 mg/LV 75 mg, 4-week administration and 1-week no-administration period) on an outpatient basis. After 2 courses, chest CT revealed reduction of both metastases, and complete resection of the metastases by video assisted thoracic surgery (VATS) was planned. Pathological findings of a specimen revealed no residual cancer cells, indicating a complete response to UFT/LV therapy. After these treatments, combined therapy of UFT/LV was continued for 3 months, and the single administration of UFT was continued for 1 year. The patient experienced no adverse reactions, and has had no recurrent disease in 4 years. Oral UFT/LV therapy is considered to be a promising regimen for patients with resectable metastatic lesion from a standpoint of clinical efficacy and safety. | Administration, Oral, Antineoplastic Combined Chemotherapy Protocols, Humans, Leucovorin, Lung Neoplasms, Male, Middle Aged, Rectal Neoplasms, Remission Induction, Tegafur, Tomography, X-Ray Computed, Uracil | null |
22,705,703 | 2012-08-14 | 2018-12-01 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of elderly locally-advanced breast cancer with skin ulcer responding to letrozole]. | Sasaki Yasuhiro, Okuyama Manabu, Hibino Masanori, Tenma Kazuo, Nakamura Masaaki | jpn | null | Case Reports, Journal Article | Antineoplastic Agents, Nitriles, Triazoles, Letrozole | IM | 22705703 | We report an 84-year-old woman with an ulcerated tumor of her right breast. The histological examination showed invasive ductal carcinoma, ER (allred-score 8), PgR (allred-score 0), and HER2 (0). Computed tomography and bone scintigraphy showed no distant metastases. She was diagnosed with right locally advanced breast cancer (cT4bN2aM0, stageIIIB) and received hormone therapy with letrozole. One year after, the carcinoma was reduced in size and the skin ulcer had disappeared. Two years later, the carcinoma had grown back slightly, but the skin ulcer was still undetectable. Hormone therapy alone was considered useful for treatment of elderly locally advanced breast cancer patients. | Aged, 80 and over, Antineoplastic Agents, Biopsy, Breast Neoplasms, Carcinoma, Ductal, Breast, Female, Humans, Letrozole, Neoplasm Grading, Neoplasm Staging, Nitriles, Skin Ulcer, Triazoles | null |
22,705,704 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A long survival of a patient with poor performance status who suffered from advanced right breast cancer with multiple lung metastases controlled by trastuzumab as a key drug]. | Fujikawa Takahisa, Mukai Kumiko, Tanaka Akira, Abe Toshihiro, Yoshimoto Yasunori, Tada Seiichiro, Maekawa Hisatsugu, Shimoike Norihiro, Tanaka Hironori, Kawashima Takashi, Yokota Tadaaki | jpn | null | Case Reports, English Abstract, Journal Article | Antibodies, Monoclonal, Humanized, Antineoplastic Agents, Trastuzumab | IM | 22705704 | We report a case of a 60-year-old woman with poor performance status (PS). She suffered from advanced right breast cancer with multiple lung metastases, which was controlled by chemotherapy with trastuzumab as the key drug. The patient presented with a 4 cm-sized large right breast mass. Her PS was poor due to progressive spinocerebellar degeneration. The biopsy specimen of the breast mass showed scirrhous type of the invasive ductal carcinoma (ER+, HER2 2+). Multiple lung metastases were also detected by computed tomography. Considering her poor PS, the patient was treated with mild systemic therapy using trastuzumab as the key drug. A different drug response was achieved between the breast mass and lung metastatic lesions, and the tumors were maintained as stable disease (SD) during first 18 months. However, she finally passed away due to respiratory failure resulting from lung metastasis, 33 months after starting treatment. The autopsy findings showed a difference of HER2 expression between the breast tumor and lung metastatic lesions. It must be recognized that differences of HER2 expression between the primary tumor and metastatic lesions are sometimes demonstrated in patients with breast cancer, and that trastuzumab can be used as a key drug in some patients as in the current case. | Antibodies, Monoclonal, Humanized, Antineoplastic Agents, Antineoplastic Combined Chemotherapy Protocols, Autopsy, Bone Neoplasms, Breast Neoplasms, Carcinoma, Ductal, Breast, Fatal Outcome, Female, Humans, Lung Neoplasms, Middle Aged, Trastuzumab | null |
22,705,705 | 2012-08-14 | 2015-11-19 | 0385-0684 | Gan to kagaku ryoho. Cancer & chemotherapy | [A case of primary breast cancer responding to pre-operative chemotherapy with the combination of paclitaxel and carboplatin for ovarian cancer]. | Kitagawa Dai, Nako Yurie, Honda Yayoi, Shigekawa Takashi, Horiguchi Kazumi, Aruga Tomoyuki, Yamashita Toshinari, Kuroi Katsumasa | jpn | null | Case Reports, English Abstract, Journal Article | Carboplatin, Paclitaxel | IM | 22705705 | A 62-year-old woman was diagnosed with primary left breast cancer during a follow-up for an ovarian tumor. She had at first undergone surgical resection of an ovarian tumor, and a pathological examination had revealed ovarian cancer. Gynecologists decided to treat her ovarian cancer with chemotherapy, and we were initially planning to provide treatment for breast cancer after that was completed. Sentinel lymph node biopsy performed before chemotherapy revealed no axillary metastases. The patient received six courses of intravenous PTX (175 mg/m2 on day 1, every 3 weeks) and intravenous CBDCA (AUC6 on day 1, every 3 weeks) as combination therapy. Abdominal lymph node dissection was performed between chemotherapy courses 3 and 4. The lump in the left breast showed partial clinical response, and partial resection of the left breast was performed after completion of chemotherapy. In Japan, few cases of primary breast cancer treated preoperatively using carboplatin-containing regimens have been described. | Adenocarcinoma, Papillary, Antineoplastic Combined Chemotherapy Protocols, Breast Neoplasms, Carboplatin, Combined Modality Therapy, Female, Humans, Middle Aged, Neoplasms, Multiple Primary, Ovarian Neoplasms, Paclitaxel, Sentinel Lymph Node Biopsy | null |
22,705,706 | 2013-01-23 | 2014-12-01 | 1532-2742 | The Journal of infection | Multicentre evaluation of central nervous system infections due to Flavi and Phleboviruses in Turkey. | Ergunay Koray, Sayiner A Arzu, Litzba Nadine, Lederer Sabine, Charrel Remi, Kreher Petra, Us Durdal, Niedrig Matthias, Ozkul Aykut, Hascelik Gulsen | eng | null | Case Reports, Journal Article, Multicenter Study | RNA, Viral | IM | 22705706, S0163-4453(12)00156-9, 10.1016/j.jinf.2012.05.010 | Flavi- and Phleboviruses associated with central nervous system (CNS) infections including West Nile Virus (WNV), Tick-borne Encephalitis Virus (TBEV) and Toscana Virus (TOSV) cause significant morbidity and mortality in humans. In this study, the impact of these agents have been investigated in CNS infections at referral hospitals in two provinces in Turkey, where circulation of these viruses have previously been recognized. | Adult, Aged, Bunyaviridae Infections, Encephalitis Viruses, Tick-Borne, Encephalitis, Tick-Borne, Female, Hospitals, Humans, Immunoassay, Male, Meningoencephalitis, Middle Aged, Neutralization Tests, Polymerase Chain Reaction, RNA, Viral, Sandfly fever Naples virus, Turkey, West Nile Fever, West Nile virus, Young Adult | null |
22,705,710 | 2013-09-05 | 2023-11-20 | 1095-9327 | Molecular and cellular neurosciences | Mitochondria and ALS: implications from novel genes and pathways. | Cozzolino Mauro, Ferri Alberto, Valle Cristiana, Carrì Maria Teresa | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | DNA-Binding Proteins, RNA-Binding Protein FUS | IM | 22705710, S1044-7431(12)00105-4, 10.1016/j.mcn.2012.06.001 | Evidence from patients with sporadic and familiar amyotrophic lateral sclerosis (ALS) and from models based on the overexpression of mutant SOD1 found in a small subset of patients, clearly point to mitochondrial damage as a relevant facet of this neurodegenerative condition. In this mini-review we provide a brief update on the subject in the light of newly discovered genes (such as TDP-43 and FUS/TLS) associated to familial ALS and of a deeper knowledge of the mechanisms of derangement of mitochondria. This article is part of a Special Issue entitled 'Mitochondrial function and dysfunction in neurodegeneration'. | Amyotrophic Lateral Sclerosis, Animals, DNA-Binding Proteins, Genes, Mitochondrial, Humans, Mitochondria, RNA-Binding Protein FUS | null |
22,705,708 | 2013-01-08 | 2013-11-21 | 1096-0295 | Regulatory toxicology and pharmacology : RTP | Assessment of genotoxic potential of Cr(VI) in the mouse duodenum: an in silico comparison with mutagenic and nonmutagenic carcinogens across tissues. | Thompson Chad M, Gregory Hixon J, Proctor Deborah M, Haws Laurie C, Suh Mina, Urban Jonathan D, Harris Mark A | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Carcinogens, Environmental, Chromates, Mutagens, Chromium, chromium hexavalent ion, sodium bichromate | IM | 22705708, S0273-2300(12)00113-4, 10.1016/j.yrtph.2012.05.019 | In vitro studies on hexavalent chromium [Cr(VI)] indicate that reduced forms of this metal can interact with DNA and cause mutations. Recently, Cr(VI) was shown to induce intestinal tumors in mice; however, Cr(VI) elicited redox changes, cytotoxicity and hyperplasia - suggesting involvement of tissue injury rather than direct mutagenesis. Moreover, toxicogenomic analyses indicated limited evidence for DNA damage responses. Herein, we extend these toxicogenomic analyses by comparing the gene expression patterns elicited by Cr(VI) with those of four mutagenic and four nonmutagenic carcinogens. To date, toxicogenomic profiles for mutagenic and nonmutagenic duodenal carcinogens do not exist, thus duodenal gene changes in mice were compared to those elicited by hepatocarcinogens. Specifically, duodenal gene changes in mice following exposure to Cr(VI) in drinking water were compared to hepatic gene changes previously identified as potentially discriminating mutagenic and nonmutagenic hepatocarcinogens. Using multivariate statistical analyses (including logistic regression classification), the Cr(VI) gene responses clustered apart from mutagenic carcinogens and closely with nonmutagenic carcinogens. These findings are consistent with other intestinal data supporting a nonmutagenic mode of action (MOA). These findings may be useful as part of a full weight of evidence MOA evaluation for Cr(VI)-induced intestinal carcinogenesis. Limitations to this analysis will also be discussed. | Animals, Carcinogens, Environmental, Chromates, Chromium, Computer Simulation, DNA Damage, Duodenum, Female, Gene Expression Regulation, Neoplastic, Genome-Wide Association Study, Intestinal Mucosa, Liver, Logistic Models, Mice, Mice, Inbred Strains, Mutagenicity Tests, Mutagens, Oligonucleotide Array Sequence Analysis, Principal Component Analysis, Risk Assessment, Toxicogenetics | null |
22,705,707 | 2013-01-08 | 2012-08-20 | 1096-0295 | Regulatory toxicology and pharmacology : RTP | The threshold of toxicological concern for prenatal developmental toxicity in rabbits and a comparison to TTC values in rats. | van Ravenzwaay B, Dammann M, Buesen R, Flick B, Schneider S | eng | null | Comparative Study, Journal Article | Teratogens, Xenobiotics | IM | 22705707, S0273-2300(12)00114-6, 10.1016/j.yrtph.2012.06.004 | The Threshold Toxicological Concern (TTC) is based on the concept that reasonable assurance of safety can be given if exposure is sufficiently low. We report on the evaluation of BASF's data for oral developmental toxicity studies in rabbits with 48 NOAEL values for maternal and developmental toxicity. The 5th percentile of the NOAEL distributions was calculated to be 5mg/kgbw/d for both maternal and developmental toxicity. From literature 56 compounds tested in rabbits were taken and combined with values from BASF's studies. The 5th percentile value for developmental toxicity of these 104 studies (mostly active ingredients) was 2mg/kgbw/d. Thus, a TTC value of 4μg/kgbw/d was calculated using a safety factor of 500 to account for relatively small database. This value is in the same range as the TTC value for developmental toxicity in rats of 8μg/kgbw/d. The lower value may serve as guidance to determine whether further evaluation is needed or whether to rely on a TTC value for industrial chemicals or low concentration (environmental) contaminants if exposure is sufficiently low. A comparison of 30 compounds tested at BASF in both species, suggests that rabbits are not more sensitive than rats. We encourage others to publish data on rabbit developmental toxicity. | Abnormalities, Drug-Induced, Animals, Dose-Response Relationship, Drug, Embryo, Mammalian, Female, Fetal Death, Fetal Development, Maternal Exposure, No-Observed-Adverse-Effect Level, Rabbits, Rats, Risk Assessment, Species Specificity, Teratogens, Toxicity Tests, Xenobiotics | null |
22,705,709 | 2012-10-25 | 2019-12-10 | 1361-6560 | Physics in medicine and biology | Auto-masked 2D/3D image registration and its validation with clinical cone-beam computed tomography. | Steininger P, Neuner M, Weichenberger H, Sharp G C, Winey B, Kametriser G, Sedlmayer F, Deutschmann H | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Validation Study | null | IM | 22705709, 10.1088/0031-9155/57/13/4277 | Image-guided alignment procedures in radiotherapy aim at minimizing discrepancies between the planned and the real patient setup. For that purpose, we developed a 2D/3D approach which rigidly registers a computed tomography (CT) with two x-rays by maximizing the agreement in pixel intensity between the x-rays and the corresponding reconstructed radiographs from the CT. Moreover, the algorithm selects regions of interest (masks) in the x-rays based on 3D segmentations from the pre-planning stage. For validation, orthogonal x-ray pairs from different viewing directions of 80 pelvic cone-beam CT (CBCT) raw data sets were used. The 2D/3D results were compared to corresponding standard 3D/3D CBCT-to-CT alignments. Outcome over 8400 2D/3D experiments showed that parametric errors in root mean square were <0.18° (rotations) and <0.73 mm (translations), respectively, using rank correlation as intensity metric. This corresponds to a mean target registration error, related to the voxels of the lesser pelvis, of <2 mm in 94.1% of the cases. From the results we conclude that 2D/3D registration based on sequentially acquired orthogonal x-rays of the pelvis is a viable alternative to CBCT-based approaches if rigid alignment on bony anatomy is sufficient, no volumetric intra-interventional data set is required and the expected error range fits the individual treatment prescription. | Automation, Cone-Beam Computed Tomography, Humans, Imaging, Three-Dimensional, Male, Prostatic Neoplasms, Radiotherapy Planning, Computer-Assisted | null |
22,705,711 | 2013-01-15 | 2022-03-16 | 1873-2194 | Progress in lipid research | Acyl-CoA:diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology. | Liu Qin, Siloto Rodrigo M P, Lehner Richard, Stone Scot J, Weselake Randall J | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Bacterial Proteins, Fungal Proteins, Triglycerides, Diacylglycerol O-Acyltransferase | IM | 22705711, S0163-7827(12)00038-0, 10.1016/j.plipres.2012.06.001 | Triacylglycerol (TG) is a storage lipid which serves as an energy reservoir and a source of signalling molecules and substrates for membrane biogenesis. TG is essential for many physiological processes and its metabolism is widely conserved in nature. Acyl-CoA:diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) catalyzes the final step in the sn-glycerol-3-phosphate pathway leading to TG. DGAT activity resides mainly in two distinct membrane bound polypeptides, known as DGAT1 and DGAT2 which have been identified in numerous organisms. In addition, a few other enzymes also hold DGAT activity, including the DGAT-related acyl-CoA:monoacylglycerol acyltransferases (MGAT). Progress on understanding structure/function in DGATs has been limited by the lack of detailed three-dimensional structural information due to the hydrophobic properties of theses enzymes and difficulties associated with purification. This review examines several aspects of DGAT and MGAT genes and enzymes, including current knowledge on their gene structure, expression pattern, biochemical properties, membrane topology, functional motifs and subcellular localization. Recent progress in probing structural and functional aspects of DGAT1 and DGAT2, using a combination of molecular and biochemical techniques, is emphasized. Biotechnological applications involving DGAT enzymes ranging from obesity therapeutics to oilseed engineering are also discussed. | Animals, Bacteria, Bacterial Proteins, Diacylglycerol O-Acyltransferase, Fungal Proteins, Fungi, Plants, Protein Structure, Tertiary, Substrate Specificity, Triglycerides | null |
22,705,712 | 2012-09-28 | 2021-12-03 | 1872-7786 | Chemico-biological interactions | Novel molecular mechanisms of antitumor action of dichloroacetate against T cell lymphoma: Implication of altered glucose metabolism, pH homeostasis and cell survival regulation. | Kumar Ajay, Kant Shiva, Singh Sukh Mahendra | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, Culture Media, Conditioned, Cytokines, Enzyme Inhibitors, Glucose Transporter Type 1, Monocarboxylic Acid Transporters, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, Reactive Oxygen Species, Slc2a1 protein, mouse, Symporters, monocarboxylate transport protein 1, Lactic Acid, Dichloroacetic Acid, Protein Serine-Threonine Kinases, Caspase 3, Glucose | IM | 22705712, S0009-2797(12)00104-4, 10.1016/j.cbi.2012.06.005 | Pyruvate dehydrogenase kinase (PDK) inhibits pyruvate dehydrogenase (PDH) activity and thus promotes energetic switch from mitochondrial glucose oxidation to cytoplasmic glycolysis in cancerous cells (a phenomenon known as the 'Warburg effect') for their energy need, which facilitates the cancer progression by resisting induction of apoptosis and promoting tumor metastasis. Thus, in the present investigation, we explored the molecular mechanisms of the tumoricidal action of dichloroacetate (DCA), a pyruvate dehydrogenase kinase inhibitor, on cells of a murine T cell lymphoma, designated as Dalton's lymphoma (DL). In vitro treatment of tumor cells with DCA inhibited their survival accompanied by a modulation of the biophysical composition of tumor-conditioned medium with respect to pH, glucose and lactate. DCA treatment also altered expression of HIF1-α and pH regulators: VATPase and MCT1 and production of cytokines: IL-10, IL-6 and IFN-γ. Moreover, we also observed an alteration in the expression of other apoptosis and cell survival regulatory molecules: PUMA, GLUT1, Bcl2, p53, CAD, caspase-3 and HSP70. The study discusses the role of novel molecular mechanisms underlying DCA-dependent inhibition of tumor cell survival. This study shows for the first time that DCA-dependent alteration of tumor cell survival involves altered pH homeostasis and glucose metabolism. Thus, these findings will provide a new insight for therapeutic applications of DCA as a novel antineoplastic agent against T cell lymphoma. | Animals, Antineoplastic Agents, Apoptosis, Caspase 3, Cell Line, Tumor, Cell Survival, Culture Media, Conditioned, Cytokines, Dichloroacetic Acid, Drug Screening Assays, Antitumor, Enzyme Inhibitors, Gene Expression Regulation, Glucose, Glucose Transporter Type 1, Hydrogen-Ion Concentration, Lactic Acid, Lymphoma, T-Cell, Male, Mice, Mice, Inbred BALB C, Monocarboxylic Acid Transporters, Protein Serine-Threonine Kinases, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, Reactive Oxygen Species, Symporters, Thymocytes | null |
22,705,714 | 2013-02-14 | 2016-05-18 | 1876-7737 | Journal of proteomics | The temporal analysis of yeast exponential phase using shotgun proteomics as a fermentation monitoring technique. | Huang Eric L, Orsat Valérie, Shah Manesh B, Hettich Robert L, VerBerkmoes Nathan C, Lefsrud Mark G | eng | null | Journal Article, Research Support, U.S. Gov't, Non-P.H.S. | Fungal Proteins, Proteome, Saccharomyces cerevisiae Proteins | IM | 22705714, S1874-3919(12)00430-7, 10.1016/j.jprot.2012.06.005 | System biology and bioprocess technology can be better understood using shotgun proteomics as a monitoring system during the fermentation. We demonstrated a shotgun proteomic method to monitor the temporal yeast proteome in early, middle and late exponential phases. Our study identified a total of 1389 proteins combining all 2D-LC-MS/MS runs. The temporal Saccharomyces cerevisiae proteome was enriched with proteolysis, radical detoxification, translation, one-carbon metabolism, glycolysis and TCA cycle. Heat shock proteins and proteins associated with oxidative stress response were found throughout the exponential phase. The most abundant proteins observed were translation elongation factors, ribosomal proteins, chaperones and glycolytic enzymes. The high abundance of the H-protein of the glycine decarboxylase complex (Gcv3p) indicated the availability of glycine in the environment. We observed differentially expressed proteins and the induced proteins at mid-exponential phase were involved in ribosome biogenesis, mitochondria DNA binding/replication and transcriptional activator. Induction of tryptophan synthase (Trp5p) indicated the abundance of tryptophan during the fermentation. As fermentation progressed toward late exponential phase, a decrease in cell proliferation was implied from the repression of ribosomal proteins, transcription coactivators, methionine aminopeptidase and translation-associated proteins. | Amino Acid Sequence, Cell Division, Cell Proliferation, Cluster Analysis, Fermentation, Fungal Proteins, Metabolic Networks and Pathways, Proteome, Proteomics, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors | null |
22,705,713 | 2012-11-06 | 2013-11-21 | 1872-7786 | Chemico-biological interactions | Involvement of the p38 MAPK and ERK signaling pathway in the anti-melanogenic effect of methyl 3,5-dicaffeoyl quinate in B16F10 mouse melanoma cells. | Shen Ting, Heo Seong-Il, Wang Myeong-Hyeon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, Phytogenic, Melanins, Microphthalmia-Associated Transcription Factor, Plant Extracts, methyl 3, 5-O-dicaffeoylquinate, Quinic Acid, Monophenol Monooxygenase, Extracellular Signal-Regulated MAP Kinases, p38 Mitogen-Activated Protein Kinases | IM | 22705713, S0009-2797(12)00103-2, 10.1016/j.cbi.2012.06.004 | Methyl 3,5-dicaffeoyl quinate (MDQ), an active compound present in Kalopanax pictus, Salicornia herbacea L., Aster oharai and Solidago virga-aurea var. gigantean, is a dicaffeoylquinic acid derivative esterified by methanol. Recent studies have revealed that MDQ possesses multiple pharmacological activities, such as antitumor, antioxidative and cytoprotective activities. To date, there has been no attempt to test the action of MDQ in melanocytes. In this study, we investigated the effect of MDQ on melanogenesis in B16F10 mouse melanoma cells. MDQ inhibited melanin production and tyrosinase activity in B16F10 mouse melanoma cells without a direct inhibitory effect on mushroom tyrosinase activity. Furthermore, we also found that MDQ decreased protein expression levels of microphthalmia-associated transcription factor (MITF) and tyrosinase in B16F10 melanin cells. Meanwhile, phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) was significantly reduced after 6h MDQ treatment, and this expression recovered at 48 h. The phosphorylation of extracellular signal-regulated kinase (ERK) was significantly enhanced at 12-48 h, whereas no effect was observed in the phosphorylation of Akt. In addition, MDQ treatment did not significantly alter the expression levels of total p38 MAPK, ERK, and Akt. Thus, it seems that inhibition of phospho-p38 MAPK and activation of phospho-ERK may lead to the suppression of melanogenesis in MDQ-treated B16F10 mouse melanoma cells. | Agaricales, Animals, Antineoplastic Agents, Phytogenic, Cell Line, Tumor, Cell Survival, Extracellular Signal-Regulated MAP Kinases, Kalopanax, MAP Kinase Signaling System, Melanins, Melanocytes, Melanoma, Mice, Microphthalmia-Associated Transcription Factor, Monophenol Monooxygenase, Phosphorylation, Plant Extracts, Quinic Acid, p38 Mitogen-Activated Protein Kinases | null |
22,705,715 | 2014-05-12 | 2024-11-27 | 1468-3318 | Tobacco control | 'The times are changing': New Zealand smokers' perceptions of the tobacco endgame. | Maubach Ninya, Hoek Janet A, Edwards Richard, Gifford Heather, Erick Stephanie, Newcombe Rhiannon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705715, tobaccocontrol-2011-050398, 10.1136/tobaccocontrol-2011-050398 | The New Zealand government's goal of achieving a smoke-free society by 2025 reflects growing interest in 'endgame' solutions to tobacco smoking. However, tobacco companies have framed 'endgame' strategies as contrary to individual freedoms and 'choice'; these claims heighten politicians' sensitivity to 'nanny state' allegations and may undermine tobacco control policies. Public support for stronger policies could strengthen political will; however, little is known about how smokers perceive endgame scenarios or the factors underlying their support or opposition to these. | Adolescent, Adult, Attitude to Health, Female, Health Policy, Health Promotion, Humans, Interviews as Topic, Male, Middle Aged, New Zealand, Perception, Personal Autonomy, Pregnancy, Smoking, Smoking Cessation, Social Values, Tobacco Industry, Young Adult, Tobacco Products | Tobacco endgame, advertising and promotion, advocacy, cessation, endgame, environmental tobacco smoke, litigation, packaging and labelling, prevalence, primary healthcare, priority populations, priority/special populations, public policy, qualitative research, smoking-caused disease, tobacco control in Africa |
22,705,716 | 2014-05-12 | 2024-05-18 | 1468-3318 | Tobacco control | The Gold Standard Programme: smoking cessation interventions for disadvantaged smokers are effective in a real-life setting. | Neumann Tim, Rasmussen Mette, Ghith Nermin, Heitmann Berit L, Tønnesen Hanne | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705716, tobaccocontrol-2011-050194, 10.1136/tobaccocontrol-2011-050194, PMC3812829, 21694504, 18936053, 15733243, 19588322, 20298324, 19638397, 15846616, 17158850, 18188745, 11809253, 17499846, 19149828, 15846610, 15533541, 18425860, 21212379, 17947786, 15755262, 16816946, 21178184, 1932883, 17253581, 18646105, 15844289 | To evaluate the real-life effect of an evidence-based Gold Standard Programme (GSP) for smoking cessation interventions in disadvantaged patients and to identify modifiable factors that consistently produce the highest abstinence rates. | Adolescent, Adult, Aged, Aged, 80 and over, Denmark, Educational Status, Female, Health Behavior, Humans, Male, Middle Aged, Program Evaluation, Prospective Studies, Smoking, Smoking Cessation, Tobacco Use Disorder, Treatment Outcome, Unemployment, Vulnerable Populations, Young Adult | Smoking, health disparities, health inequalities, nationwide database, smoking cessation intervention |
22,705,717 | 2012-10-30 | 2024-06-10 | 1524-4040 | Neurosurgery | Mapping the human connectome. | Toga Arthur W, Clark Kristi A, Thompson Paul M, Shattuck David W, Van Horn John Darrell | eng | P41 EB015922 (NIBIB NIH HHS, United States); U01 MH093765 (NIMH NIH HHS, United States); K99 HD065832 (NICHD NIH HHS, United States); R01 MH094343 (NIMH NIH HHS, United States); 9P41EB015922 (NIBIB NIH HHS, United States); P41 RR013642 (NCRR NIH HHS, United States); P41RR013642 (NCRR NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705717, 10.1227/NEU.0b013e318258e9ff, 00006123-201207000-00001, PMC3555558, NIHMS377780, 9623998, 19683061, 16247738, 18982600, 19212449, 8019776, 22500773, 22903354, 20016083, 17271286, 21497128, 25346947, 25404993, 17396125, 16685847, 18219621, 12778119, 15319512, 14645885, 11771995, 22903274, 21850668, 18597554, 10571934, 19190637, 6586380, 21376655, 22903203, 19694286, 20426114, 12480486, 15906294, 19784379, 11025519, 20950689, 19515930, 23286144, 19492086, 19063977, 18037310, 17481925, 9989633, 15850749, 25404992, 18801435, 22903411, 18041271, 12489094, 18446160, 17931890 | Knowledge of the properties of white matter fiber tracts isa crucial and necessary step toward a precise understanding of the functional architecture of the living human brain. Previously, this knowledge was severely limited, as it was difficult to visualize these structures or measure their functions in vivo. The HCP has recently generated considerable interest because of its potential to explore connectivity and its relationship with genetics and behavior. For neuroscientists and the lay public alike, the ability to assess, measure, and explore this wealth of layered information concerning how the brain is wired is a much sought after prize.The navigation of the human connectome and the discovery of how it is affected through genetics, and in a range of neurological and psychiatric diseases, have far reaching implications. From a range of ongoing connectomics related activities, the systematic characterization of brain connectedness and the resulting functional aspects of such connectivity will not only realize the work of Ramón y Cajal and others, but will also greatly expand our understanding of the brain, the mind, and what it is to be truly human. The similarities and differences that mark normal diversity will help us to understand variation among people and set the stage to chart genetic influences on typical brain development and decline during aging. What is more, an understanding of how brains might become disordered will shed light on autism, schizophrenia, Alzheimer’s, and other diseases that exact a tremendous and terrible social and economic toll. | Brain, Brain Mapping, Humans, Image Processing, Computer-Assisted, Nerve Fibers, Myelinated, Neural Pathways, Neuroimaging | null |
22,705,718 | 2012-10-30 | 2012-06-18 | 1524-4040 | Neurosurgery | Preuntethering and postuntethering courses of syringomyelia associated with tethered spinal cord. | Lee Ji Yeoun, Phi Ji Hoon, Cheon Jung-Eun, Kim Seung-Ki, Kim In-One, Cho Byung-Kyu, Wang Kyu-Chang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705718, 10.1227/NEU.0b013e31824cebc6, 00006123-201207000-00004 | There has been controversy regarding the management of syringomyelia associated with tethered spinal cord. Previous reports on the topic have included only a small number of patients, considered open/closed spinal dysraphism together, or had a short follow-up. | Adolescent, Chi-Square Distribution, Child, Child, Preschool, Disability Evaluation, Disease Progression, Female, Follow-Up Studies, Humans, Infant, Infant, Newborn, Magnetic Resonance Imaging, Male, Neural Tube Defects, Retrospective Studies, Syringomyelia, Tomography Scanners, X-Ray Computed, Treatment Outcome | null |
22,705,719 | 2012-10-30 | 2012-06-18 | 1524-4040 | Neurosurgery | Interrater and intrarater reliability of the Kuntz et al new deformity classification system. | Thaler Martin, Lechner Ricarda, Gstöttner Michaela, Luegmair Matthias, Liebensteiner Michael, Nogler Michael, Bach Christian | eng | null | Journal Article | null | IM | 22705719, 10.1227/NEU.0b013e31824f4e58, 00006123-201207000-00010 | Kuntz et al recently introduced a new system for classifying spinal deformities. This classification of spinal deformity was developed from age-dependent deviations from the neutral upright spinal alignment. | Adolescent, Adult, Age Factors, Aged, Child, Female, Humans, Male, Middle Aged, Reproducibility of Results, Spinal Diseases, Spine, Young Adult | null |
22,705,720 | 2012-10-30 | 2021-12-09 | 1524-4040 | Neurosurgery | Subdural hematoma in patients with cancer. | Reichman Jordan, Singer Samuel, Navi Babak, Reiner Anne, Panageas Kathy, Gutin Philip H, Deangelis Lisa M | eng | null | Journal Article | null | IM | 22705720, 10.1227/NEU.0b013e3182517938, 00006123-201207000-00013 | Subdural hematoma (SDH) in patients with cancer is poorly described, and its frequency and causes may have changed with recent oncologic advances. | Adolescent, Adult, Aged, Aged, 80 and over, Confidence Intervals, Female, Hematoma, Subdural, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Neoplasms, Proportional Hazards Models, Retrospective Studies, Tomography Scanners, X-Ray Computed, Treatment Outcome, Young Adult | null |
22,705,724 | 2012-12-14 | 2013-11-21 | 1873-7544 | Neuroscience | Mechanisms mediating effects of nitric oxide on perifornical lateral hypothalamic neurons. | Kostin A, McGinty D, Szymusiak R, Alam M N | eng | MH63323 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | Neurotransmitter Agents, Nitric Oxide | IM | 22705724, S0306-4522(12)00614-8, 10.1016/j.neuroscience.2012.06.014 | The perifornical-lateral hypothalamic area (PF-LHA) is a major wake-promoting structure. It predominantly contains neurons that are active during behavioral and cortical activation. Nitric oxide (NO) is a gaseous neurotransmitter that has been implicated in the regulation of sleep. Recently we found that NO levels in the PF-LHA are higher during sustained waking and that NO exerts predominantly inhibitory effects, especially on PF-LHA neurons excited by tactile stimulation. The mechanisms underlying this NO-evoked inhibitory action on the PF-LHA neurons were assessed in the present study. We investigated the contributions of adenosinergic, GABAergic, and sGC-cGMP signaling mechanisms in mediating nitrergic influences on the PF-LHA neurons. The extracellular discharge activity of PF-LHA neurons was recorded in combination with microdialytic delivery of pharmacological agents adjacent to the recorded neurons in urethane-anesthetized rats. First, we quantified changes in the discharge activity of the PF-LHA neurons during the blockade of the adenosine A(1) receptor, GABA(A) receptor, and sGC-cGMP pathway. Then, we determined the efficacy of blocking adenosine A(1) receptor, GABA(A) receptor, and sGC signaling mechanisms in attenuating the inhibitory influences of 3,3-bis(aminoethyl)-1-hydroxy-1-oxo-1-triazene (a NO donor) (NOC-18), a NO donor, on the discharge activity of the PF-LHA neurons. We found that NOC-18-induced suppression in the discharge activity of PF-LHA neurons was significantly attenuated during the blockade of adenosine A(1) receptor-, GABA(A) receptor-, and sGC-cGMP-mediated signaling. These findings suggest that NO-evoked inhibition of PF-LHA neurons involves a complex mechanism including, but may not be limited to, adenosinergic, GABAergic and sGC-cGMP signaling pathways. The findings are consistent with a generalized sleep-promoting role of NO within the PF-LHA and, given the sleep-promoting roles of adenosinergic and GABAergic systems in this area, further suggest that this effect may be mediated through nitrergic interactions with other neurotransmitters and neuromodulators. | Action Potentials, Animals, Electroencephalography, Hypothalamic Area, Lateral, Male, Neurons, Neurotransmitter Agents, Nitric Oxide, Rats, Rats, Sprague-Dawley, Signal Transduction | null |
22,705,725 | 2012-10-23 | 2022-12-07 | 1536-3708 | Annals of plastic surgery | Management of tattoos in the operative field: how to save a gecko. | Tenna Stefania, Delle Femmine Pietro Francesco, Filoni Angela, Cagli Barbara, Brunetti Beniamino, Persichetti Paolo | eng | null | Letter, Comment | null | IM | 22705725, 10.1097/SAP.0b013e31824ca564 | null | Dermatologic Surgical Procedures, Humans, Plastic Surgery Procedures, Tattooing | null |
22,705,726 | 2012-10-23 | 2012-06-20 | 1536-3708 | Annals of plastic surgery | Surgical technique of a partial tibial nerve transfer to the tibialis anterior motor branch for the treatment of peroneal nerve injury. | Giuffre Jennifer L, Bishop Allen T, Spinner Robert J, Shin Alexander Y | eng | null | Journal Article | null | IM | 22705726, 10.1097/SAP.0b013e31824c94e5 | Injuries to the common peroneal nerve can be functionally debilitating with few treatment options. Traditionally, tendon transfers and ankle-foot orthotics have been the standard treatment of foot drop with satisfactory patient outcomes. The purpose of this manuscript is to describe an alternative surgical technique option to obtain ankle dorsiflexion in patients with foot drop using a partial nerve transfer from the tibial nerve to the motor branch of the tibialis anterior. | Gait Disorders, Neurologic, Humans, Nerve Transfer, Peripheral Nerve Injuries, Peroneal Nerve, Tibial Nerve | null |
22,705,727 | 2012-10-30 | 2021-10-21 | 1745-7254 | Acta pharmacologica Sinica | Tissue cholesterol content alterations in streptozotocin-induced diabetic rats. | Wang Xin-ting, Li Jia, Liu Li, Hu Nan, Jin Shi, Liu Can, Mei Dan, Liu Xiao-dong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters, Apolipoproteins E, Insulin, Liver X Receptors, Orphan Nuclear Receptors, RNA, Messenger, Cholesterol | IM | 22705727, aps201250, 10.1038/aps.2012.50, PMC4011154, 15589694, 11881745, 20835267, 20713354, 15716586, 14938387, 17575085, 18559657, 17034661, 10585467, 21467629, 21426923, 15831500, 2066673, 15855566, 16020748, 16182288, 3904728, 20170744, 21402379, 17982015, 6177997, 3989391, 16107507, 18804448, 17074306, 14424351, 18752266, 19489738, 20172862, 9763528, 19729450, 16936198, 19965614, 19426784, 16154115, 21447785, 18390541, 17884740, 10918070, 18401018, 12351428, 19837721, 21109190, 10886564, 16198427, 18772361, 11846609, 19893012, 9519937, 10570128, 18815436, 4986214 | Diabetes is associated with elevated serum total cholesterol level and disrupted lipoprotein subfractions. The aim of this study was to examine alterations in the tissue cholesterol contents closely related to diabetic complications. | ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters, Animals, Apolipoproteins E, Cholesterol, Diabetes Mellitus, Experimental, Gene Expression, Insulin, Liver X Receptors, Male, Orphan Nuclear Receptors, RNA, Messenger, Rats, Rats, Sprague-Dawley | null |
22,705,728 | 2012-10-30 | 2021-10-21 | 1745-7254 | Acta pharmacologica Sinica | Excess genistein suppresses the synthesis of extracellular matrix in female rat mandibular condylar cartilage. | Yu Shi-bin, Xing Xiang-hui, Dong Guang-ying, Weng Xi-li, Wang Mei-qing | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Estrogen Receptor alpha, Estrogen Receptor beta, Phytoestrogens, Estradiol, Genistein | IM | 22705728, aps201249, 10.1038/aps.2012.49, PMC4011151, 19264293, 10543025, 10329332, 16213646, 15501350, 15636169, 9492349, 2662920, 12847685, 8862099, 10670598, 22120966, 12973717, 14664520, 14723875, 17368882, 17433519, 9458216, 19782341, 16546639, 19234664, 19238303, 20347979, 9486006, 18509682, 16708833, 9631171, 10428321, 9409268 | To investigate the effect of excess genistein on the extracellular matrix in mandibular condylar cartilage of female rats in vivo. | Animals, Cartilage, Cell Proliferation, Chondrocytes, Estradiol, Estrogen Receptor alpha, Estrogen Receptor beta, Extracellular Matrix, Female, Genistein, Mandibular Condyle, Phytoestrogens, Rats, Rats, Sprague-Dawley | null |
22,705,729 | 2012-10-30 | 2021-12-03 | 1745-7254 | Acta pharmacologica Sinica | Arctigenin alleviates ER stress via activating AMPK. | Gu Yuan, Sun Xiao-xiao, Ye Ji-ming, He Li, Yan Shou-sheng, Zhang Hao-hao, Hu Li-hong, Yuan Jun-ying, Yu Qiang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Furans, Lignans, Palmitates, Protein Kinases, AMP-Activated Protein Kinase Kinases, arctigenin | IM | 22705729, aps201260, 10.1038/aps.2012.60, PMC4011149, 15486293, 15705855, 18285556, 17268797, 9519740, 21922249, 14709557, 20232300, 11500364, 14651849, 19429312, 12438433, 8386634, 20981575, 15313439, 15241937, 20717870, 11779464, 4692841, 18481955, 10677345, 14970221, 16452235, 20606313, 12960015, 19043451, 12110171, 18048764, 17497122, 17041626, 18367660, 15509864, 21887385, 12086935, 15759047, 9834179, 20383171, 17565364, 16227605, 16148948, 20038265, 10828822 | To investigate the protective effects of arctigenin (ATG), a phenylpropanoid dibenzylbutyrolactone lignan from Arctium lappa L (Compositae), against ER stress in vitro and the underlying mechanisms. | AMP-Activated Protein Kinase Kinases, Animals, Arctium, Endoplasmic Reticulum Stress, Enzyme Activation, Furans, Hep G2 Cells, Humans, Insulin-Secreting Cells, Lignans, Male, Palmitates, Protein Biosynthesis, Protein Kinases, Rats, Rats, Wistar, Signal Transduction, Unfolded Protein Response | null |
22,705,731 | 2012-10-30 | 2021-10-21 | 1745-7254 | Acta pharmacologica Sinica | Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy. | Shi Ji, Yin Ning, Xuan Ling-ling, Yao Chun-suo, Meng Ai-min, Hou Qi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antioxidants, FOXO3 protein, human, Forkhead Box Protein O3, Forkhead Transcription Factors, Stilbenes, Sirtuin 1, Resveratrol | IM | 22705731, aps201273, 10.1038/aps.2012.73, PMC4011152, 20214495, 15023334, 20105289, 21721950, 17224625, 21766606, 21351489, 20493163, 19648934, 19001599, 21912480, 18830406, 22463380, 11551564, 20450879, 19200883, 21439932, 14976264, 21669942, 20801891, 20064603, 20160144, 20078221, 20618067, 20335654, 17041012, 22349133, 21778238, 15691653, 25269161, 18769149, 16723093, 21690325, 18054315, 19249351, 21238503, 20060806, 18174544, 19701188 | To appraise the efficacy of Vam3 (Amurensis H), a dimeric derivative of resveratrol, at inhibiting cigarette smoke-induced autophagy. | Animals, Antioxidants, Autophagy, Bronchi, Cell Line, Epithelial Cells, Forkhead Box Protein O3, Forkhead Transcription Factors, Gene Expression Regulation, Humans, Lung, Male, Mice, Mice, Inbred BALB C, Oxidative Stress, Pulmonary Disease, Chronic Obstructive, Resveratrol, Sirtuin 1, Smoking, Stilbenes | null |
22,705,732 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Isolation and genetic characterization of Japanese encephalitis virus from equines in India. | Gulati Baldev R, Singha Harisankar, Singh Birendra K, Virmani Nitin, Kumar Sanjay, Singh Raj K | eng | null | Journal Article | Antibodies, Monoclonal, RNA, Viral | IM | 22705732, 201206111, 10.4142/jvs.2012.13.2.111, PMC3386335, 10790108, 8866989, 19787040, 8525395, 17377815, 16637405, 17135501, 1322071, 10810598, 1321546, 12584335, 2273391, 19776238, 8702029, 17073095, 14205897, 11561712, 15332484, 7975862, 13677370, 13571577, 17325361, 15239304, 16598178, 680915, 10993935, 15166447, 1667406, 9914682, 19552100, 17488738, 12449242, 21291622, 20132860, 15528700, 16085723, 6263798 | Japanese encephalitis (JE) is an important vector-borne viral disease of humans and horses in Asia. JE outbreaks occur regularly amongst humans in certain parts of India and sporadic cases occur among horses. In this study, JE seroprevalence and evidence of JE virus (JEV) infection among horses in Haryana (India) is described. Antibodies against JEV were detected in 67 out of 637 (10.5%) horses screened between 2006 and 2010. Two foals exhibiting neurological signs were positive for JEV RNA by RT-PCR; JEV was isolated from the serum of one of the foals collected on the second day of illness. This is the first report of JEV isolation from a horse in India. Furthermore, a pool of mosquitoes collected from the premises housing these foals was positive for JEV RNA by RT-PCR. Three structural genes, capsid (C), premembrane (prM), and envelope (E) of the isolated virus (JE/eq/India/H225/2009) spanning 2,500 nucleotides (from 134 to 2,633) were cloned and sequenced. BLAST results showed that these genes had a greater than 97% nucleotide sequence identity with different human JEV isolates from India. Phylogenetic analysis based on E-and C/prM genes indicated that the equine JEV isolate belonged to genotype III and was closely related to the Vellore group of JEV isolates from India. | Animals, Antibodies, Monoclonal, Cloning, Molecular, Culex, Encephalitis Virus, Japanese, Encephalitis, Japanese, Enzyme-Linked Immunosorbent Assay, Female, Genes, Viral, Genotype, Horse Diseases, Horses, India, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Seroepidemiologic Studies | null |
22,705,730 | 2012-10-30 | 2021-12-03 | 1745-7254 | Acta pharmacologica Sinica | Atorvastatin enhances neurite outgrowth in cortical neurons in vitro via up-regulating the Akt/mTOR and Akt/GSK-3β signaling pathways. | Jin Ying, Sui Hai-juan, Dong Yan, Ding Qi, Qu Wen-hui, Yu Sheng-xue, Jin Ying-xin | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Heptanoic Acids, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Pyrroles, Atorvastatin, Glycogen Synthase Kinase 3 beta, Gsk3b protein, rat, Proto-Oncogene Proteins c-akt, TOR Serine-Threonine Kinases, Glycogen Synthase Kinase 3 | IM | 22705730, aps201259, 10.1038/aps.2012.59, PMC4011150, 11691836, 9218810, 15314020, 11489932, 16339025, 11799119, 11296263, 9636226, 16765377, 16168661, 16221682, 18266936, 18260796, 15748157, 12783420, 19224573, 10579915, 9405468, 10973320, 12906785, 11796756, 10504342, 15650333, 16280363, 17308353, 12504836, 11057898, 17179158, 16573653, 20615870, 8599949, 15194109, 19504044, 15951198, 20851742, 11527574, 8052307, 18988856, 16958588, 12820960, 15716855, 19782682, 8978681, 9465032, 16339024, 11756682, 15718470, 19574841, 16361255, 12150915, 8524413, 15647781, 15030386 | To investigate whether atorvastatin can promote formation of neurites in cultured cortical neurons and the signaling mechanisms responsible for this effect. | Animals, Atorvastatin, Cells, Cultured, Cerebral Cortex, Enzyme Activation, Glycogen Synthase Kinase 3, Glycogen Synthase Kinase 3 beta, Heptanoic Acids, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Neurites, Neurons, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Pyrroles, Rats, Rats, Sprague-Dawley, Signal Transduction, TOR Serine-Threonine Kinases | null |
22,705,733 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay. | Cha Sang-Ho, Kim Sung-Hee, Bischoff Karyn, Kim Hyun-Jeong, Son Seong-Wan, Kang Hwan-Goo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antibodies, Monoclonal, Aminooxyacetic Acid, Serum Albumin, Bovine, Zearalenone | IM | 22705733, 201206119, 10.4142/jvs.2012.13.2.119, PMC3386336, 20873582, 6218782, 11962219, 19575188, 18036606, 8407669, 15853369, 18608510, 7819759, 4143109, 16758977, 17045381, 17454110, 19190413, 6158484, 2961013, 2948167, 19825386, 7664933, 19345763 | A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to have a higher affinity for ZEN compared to a commerciall ZEN antibody. We developed two direct competitive ELISA systems using the selected antibody (ZEN-coated and anti-ZEN antibody-coated ELISA). Quantitative ranges for the anti-ZEN antibody coated ELISA and ZEN-coated ELISA were from 25 to 750 ppb and from 12.5 to 100 ppb, respectively. The detection limit of both methods as measured with standard solutions was 10 ppb. The intra-plate and inter-well variation of both ELISAs were less than 10%. The IC(50) values for α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol compared to ZEN were 108.1, 119.3, 114.1, and 130.3% for the ZEN-coated ELISA. These values were 100.7, 120.7, 121.6, and 151.6% for the anti-ZEN antibody-coated ELISA. According to the anti-ZEN antibody-coated ELISA, the average recovery rates of ZEN from spiked animal feed containing 150 to 600 ng/mL of ZEN ranged from 106.07 to 123.00% with 0.93 to 2.28% coefficients of variation. Our results demonstrate that the mAb developed in this study could be used to simultaneously screen for ZEN and its metabolites in feed. | Aminooxyacetic Acid, Animals, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Female, Inhibitory Concentration 50, Mice, Mice, Inbred BALB C, Reproducibility of Results, Serum Albumin, Bovine, Zearalenone | null |
22,705,734 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle. | Choi Sangho, Woo Hee-Jong, Lee Jeongmin | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Prions, DNA | IM | 22705734, 201206127, 10.4142/jvs.2012.13.2.127, PMC3386337, 18808703, 21120616, 18698343, 9811807, 14727152, 15890824, 9101476, 17014722, 6801762, 17709775, 8098922, 16141216, 20140032, 18625065, 17437640, 12004059, 15520885, 16253245, 15539564, 16860868, 19875887, 17426768, 8750875, 9333239, 10611351, 10051591, 7909834, 19417551, 10582288, 15354869, 16391566, 9748215, 16284424, 19917050, 10547693, 16462113 | Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native breed in Korea. We identified mutations and polymorphisms in the coding region of PRNP, determined their frequency, and evaluated their significance. We identified four synonymous polymorphisms and two non-synonymous mutations in PRNP, but found no novel polymorphisms. The sequence and number of octapeptide repeats were completely conserved, and the haplotype frequency of the coding region was similar to that of other B. taurus strains. When we examined the 23-bp and 12-bp insertion/deletion (indel) polymorphisms in the non-coding region of PRNP, Hanwoo cattle had a lower deletion allele and 23-bp del/12-bp del haplotype frequency than healthy and BSE-affected animals of other strains. Thus, Hanwoo are seemingly less susceptible to BSE than other strains due to the 23-bp and 12-bp indel polymorphisms. | Animals, Base Sequence, Cattle, DNA, Encephalopathy, Bovine Spongiform, Genetic Variation, Haplotypes, Prions, Republic of Korea | null |
22,705,737 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents. | El Behiry Ayman, Schlenker Gerd, Szabo Istvan, Roesler Uwe | eng | null | Journal Article | Anti-Bacterial Agents, Disinfectants, Nonoxynol, Iodine, Chlorhexidine | IM | 22705737, 201206153, 10.4142/jvs.2012.13.2.153, PMC3386340, 17855722, 712607, 19273638, 12682941, 9722471, 18832320, 10877387, 10791803, 19246121, 20557495, 16978433, 15328265, 16614254, 19462641, 11500230, 8107066, 9149974, 17897935, 11432424, 12000619, 18848410, 17699035, 9818977, 15860550, 10639342, 3774870, 2229606, 18665227, 18416704, 2745808, 17582107, 9880479 | Sensitivity to commercial teat dips (nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance induction of S. aureus to chemical disinfectants. Ten different strains were repeatedly passed ten times in growth media with sub-lethal concentrations of disinfectants. Nine strains showed a significant reduction in susceptibility to the nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G. Based on these results, resistance of S. aureus to chemical disinfectants may be more likely to develop if the chemicals are used at concentrations lower than that required for an optimal biocidal effect. | Animals, Anti-Bacterial Agents, Cattle, Chlorhexidine, Disinfectants, Dose-Response Relationship, Drug, Drug Resistance, Bacterial, Female, Germany, Iodine, Mastitis, Bovine, Nonoxynol, Staphylococcal Infections, Staphylococcus aureus | null |
22,705,735 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions. | Regua-Mangia Adriana Hamond, Gonzalez Alice Gonçalves M, Cerqueira Aloysio M F, Andrade João Ramos C | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Genetic Markers, Shiga Toxin 1, Shiga Toxin 2 | IM | 22705735, 201206139, 10.4142/jvs.2012.13.2.139, PMC3386338, 1569356, 17506902, 19273635, 11211268, 18366637, 11432813, 11684782, 10894541, 11756984, 8202027, 7682992, 2644022, 9284155, 11971785, 12554103, 9199437, 9986802, 17576759, 14720492, 14760970, 8795238, 8877329, 12354854, 14987734, 20806245 | Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and infection mainly occurs after ingestion of contaminated water and food. Virulence genetic markers of 28 O157:H7 strains were investigated and multilocus enzyme electrophoresis (MLEE) was used to evaluate the clonal structure. O157:H7 strains from several countries were isolated from food, human and bovine feces. According to MLEE, O157:H7 strains clustered into two main clonal groups designated A and B. Subcluster A1 included 82% of the O157:H7 strains exhibiting identical MLEE pattern. Most enterohemorrhagic E. coli (EHEC)O157:H7 strains from Brazil and Argentina were in the same MLEE subgroup. Bovine and food strains carried virulence genes associated with EHEC pathogenicity in humans. | Animals, Argentina, Brazil, Cattle, Cattle Diseases, Enterohemorrhagic Escherichia coli, Escherichia coli O157, Food Microbiology, Gene Expression Regulation, Bacterial, Genetic Markers, Humans, Polymerase Chain Reaction, Shiga Toxin 1, Shiga Toxin 2, Virulence | null |
22,705,736 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Pathotyping avian pathogenic Escherichia coli strains in Korea. | Jeong Yong-Wun, Kim Tae-Eun, Kim Jae-Hong, Kwon Hyuk-Joon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Bacterial Proteins | IM | 22705736, 201206145, 10.4142/jvs.2012.13.2.145, PMC3386339, 3539802, 18820066, 14330179, 1382824, 9683502, 9515912, 18597955, 9188088, 12933851, 15530743, 17687022, 9498443, 8757842, 18770374, 10858231, 18622850, 1427004, 160892, 16385064, 9533087, 16150559, 1699928, 20228098, 11257548, 10367360, 2241678, 9453599, 12506201, 15992275, 11010916, 10737641, 8132344, 8104335, 12781478, 18939625, 16094833, 12243519, 18842938 | To examine the genetic background of avian pathogenic Escherichia coli (APEC) that affects virulence of this microorganism, we characterized the virulence genes of 101 APEC strains isolated from infected chickens between 1985 ˜ 2005. Serotypes were determined with available anti-sera and median lethal doses were determined in subcutaneously inoculated chicks. The virulence genes we tested included ones encoding type 1 fimbriae (fimC), iron uptake-related (iroN, irp2, iucD, and fyuA), toxins (lt, st, stx1, stx2, and vat), and other factors (tsh, hlyF, ompT, and iss). Twenty-eight strains were found to be O1 (2.0%), O18 (3.0%), O20 (1.0%), O78 (19.8%), and O115 (2.0%) serotypes. The iroN (100%) gene was observed most frequently followed by ompT (94.1%), fimC (90.1%), hlyF (87.1%), iss (78.2%), iucD (73.3%), tsh (61.4%), fyuA (44.6%), and irp2 (43.6%). The strains were negative for all toxin genes except for vat (10.9%). All the strains were classified into 27 molecular pathotypes (MPs). The MP25, MP19, and MP10 pathotypes possessing iroN-fimC-ompT-hlyF-iucD-tsh-iss-irp2-fyuA (22.8%), iroN-fimC-ompT-hlyF-iucD-tsh-iss (21.8%), and iroN-fimC-ompT-hlyF-iss (11.9%) genotypes, respectively, were predominant. Redundancy of iron uptake-related genes was clearly observed and some strains were associated with higher mortality than others. Therefore, strains with the predominant genotypes can be used for diagnosis and vaccine. | Animals, Bacterial Proteins, Chickens, Escherichia coli, Escherichia coli Infections, Gene Expression Regulation, Bacterial, Phylogeny, Poultry Diseases, Republic of Korea, Virulence | null |
22,705,738 | 2012-10-22 | 2021-12-03 | 1976-555X | Journal of veterinary science | Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types. | Theerawatanasirikul Sirin, Suriyaphol Gunnaporn, Thanawongnuwech Roongroje, Sailasuta Achariya | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Complementary, Filaggrin Proteins, Intermediate Filament Proteins, Keratin-5, Protein Precursors, Keratin-10, involucrin, RNA | IM | 22705738, 201206163, 10.4142/jvs.2012.13.2.163, PMC3386341, 19659537, 7687298, 16029326, 2385171, 19043850, 2353419, 18824265, 1688904, 18166499, 21414049, 18408746, 20178492, 11553388, 19386895, 15175033, 18177286, 20187918, 20626465, 15803139, 7134655, 20178493, 10805985, 16417229, 11217052, 18927952, 18620132, 16844072, 20109730, 1601889, 9292070, 9740250, 21373745 | The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5), and their mRNA expression. This was done in the skin of five breeds of dogs including seven poodles, six golden retrievers, six Shih Tzus, four pugs, and four Labrador retrievers. Epidermal thickness of the stratum corneum and nucleated epidermal layer was significantly different. The greatest thickness was observed in the digital web area and the thinnest epidermis was in the axilla. Epidermal thickness was also significantly different between the breeds (p < 0.05). Immunohistochemical staining scores revealed significant decreases of involucrin, filaggrin, and keratin 10 in the ventral and weight-bearing sites, and a relative increase of keratin 5 (p < 0.05). q-PCR analysis showed that their the levels of mRNA were positively correlated with expression of the corresponding proteins in skin samples (p < 0.05). The present study is the first to report the relationship between epidermal gene expression and histologic morphology of the skin in normal dogs. Further studies will be essential to fully understand the pathogenesis of skin barrier dysfunctions in canines. | Animals, DNA, Complementary, Dogs, Filaggrin Proteins, Gene Expression Regulation, Intermediate Filament Proteins, Keratin-10, Keratin-5, Polymerase Chain Reaction, Protein Precursors, RNA, Skin | null |
22,705,739 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | The role of inflammation and matrix metalloproteinases in equine endometriosis. | Aresu Luca, Benali Silvia, Giannuzzi Diana, Mantovani Roberto, Castagnaro Massimo, Falomo Maria Elena | eng | null | Journal Article | Matrix Metalloproteinases | IM | 22705739, 201206171, 10.4142/jvs.2012.13.2.171, PMC3386342, 20540754, 19101039, 16405877, 21333362, 9357997, 7026016, 18468817, 12730128, 11757910, 11277878, 21726449, 1060802, 621166, 16822550, 22520817, 19022687, 15363807, 21210343, 1884699, 15137052, 16136493, 11544020, 10221716 | Equine endometriosis is a multifactorial disease considered to be a major cause of equine infertility. The purpose of this study was to evaluate the reliability of histomorphological grading for biopsy-like samples compared to entire uterine wall samples, to examine the association between the degree of endometriosis with animal age, and to investigate the role of inflammation in endometriosis and the expression of different matrix metalloproteinases in equine endometrium. Histomorphological lesions in 35 uterine samples were examined while comparing biopsy-like samples and entire-wall samples. Seventeen uterine samples were stained with antibodies against MMP-2, MMP-9, MMP-14, and TIMP-2. The morphologic evaluation results of the biopsy-like tissue and entire-wall samples were significantly correlated. Endometriosis in older mares (>12 years of age) was more severe than in young mares (2 ~ 4 years of age), confirming the positive correlation between animal age and disease severity, while inflammation was poorly related to the degree of endometriosis. MMP-2 and MMP-14 were detected in stromal cells, while MMP-9 and TIMP-2 were both found in stromal and glandular epithelial cells. There were no significant differences in MMPs expression between the two groups (young vs. old mares). Additional studies on the activity of MMPs could further define the role of these enzymes in equine endometriosis. | Animals, Endometriosis, Female, Gene Expression Regulation, Enzymologic, Horse Diseases, Horses, Immunohistochemistry, Inflammation, Matrix Metalloproteinases, Uterus | null |
22,705,740 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | C-reactive protein as an indicator of inflammatory responses to experimentally induced cystitis in dogs. | Seo Kyoung-won, Lee Jong-bok, Ahn Jin-Ok, Lee Hee-woo, Hwang Cheol-yong, Youn Hwa-young, Lee Chang-woo | eng | null | Journal Article | Anti-Bacterial Agents, Amoxicillin-Potassium Clavulanate Combination, C-Reactive Protein | IM | 22705740, 201206179, 10.4142/jvs.2012.13.2.179, PMC3386343, 8067607, 12197798, 15902658, 16962655, 8711864, 7524694, 7041546, 11731648, 16734101, 17213695, 10525124, 19298608, 11072866, 2464844, 9685461, 12774968, 15347619, 1093351, 11131585, 18196731, 18087632, 7046579, 17375337, 12471137, 19377227, 8212527, 3800121, 7024224 | The aim of this study was to demonstrate and assess C-reactive protein (CRP) changes in dogs with induced bacterial cystitis with or without antibiotics. We also evaluated availability of CRP levels to serve as an indicator for monitoring or diagnosing bacterial cystitis. Serial CRP concentrations in dogs with induced bacterial cystitis were higher than those of controls (p < 0.001). CRP concentrations peaked on day 7 and gradually decreased thereafter. In the treatment group, CRP concentrations decreased after medication compared to the untreated group (p = 0.032). CRP levels had a linear correlation with urine white blood cell counts among all groups (r = 0.837, p < 0.001, n = 140). Compared to the negative urine culture group, dogs with positive urine culture results had higher CRP concentrations (median 43.8 mg/L vs. 5.9 mg/L; p < 0.001). Area under the receiver operating characteristic curve was 0.955; when cut-off value was 12.2 mg/L, CRP measurements were found to have a sensitivity of 92.3% and specificity of 86.4%. This result indicates that rapid increases of CRP occurred after inducing bacterial cystitis and CRP may be a useful indicator for monitoring or diagnosing canine bacterial cystitis together with sediment urinalysis and urine bacterial culture. | Amoxicillin-Potassium Clavulanate Combination, Animals, Anti-Bacterial Agents, C-Reactive Protein, Cystitis, Dogs, Gene Expression Regulation, Inflammation, Male, Proteus Infections, Proteus mirabilis | null |
22,705,741 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Evaluation of the effect of a 0.0584% hydrocortisone aceponate spray on clinical signs and skin barrier function in dogs with atopic dermatitis. | Nam Eui-Hwa, Park Seol-Hee, Jung Ji-Young, Han Seung-Hee, Youn Hwa-Young, Chae Jun-Seok, Hwang Cheol-Yong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Inflammatory Agents, hydrocortisone aceponate, Hydrocortisone | IM | 22705741, 201206187, 10.4142/jvs.2012.13.2.187, PMC3386344, 20178491, 20187914, 19171021, 2723540, 18772562, 18620132, 12709124, 19374721, 11553378, 17298428, 16796631, 2007790, 18477326, 16092003, 16364034, 19659537, 11817968, 7490367, 18034533, 21174234, 15284402, 11553388, 16409433, 18249438, 11553394, 9076903, 19573156, 11553382, 16815133, 19343096, 12791047, 19757562, 19796162, 20456716, 17409646, 11553393, 11553375 | The purpose of this study was to evaluate the effects of a topical spray containing 0.0584% hydrocortisone aceponate (HCA) on canine atopic dermatitis (CAD) and to evaluate the skin barrier function during the treatment of CAD. Twenty-one dogs that fulfilled the diagnostic criteria for CAD were included in this study. The HCA spray was applied once a day to the lesions of all dogs for 7 or 14 days. Clinical assessment was performed before (day 0) and after treatment (day 14), and clinical responses were correlated with changes in skin barrier function. CAD severity significantly decreased after 14 days of HCA treatment based on the lesion scores (p < 0.0001), which were determined using the CAD extent and severity index (CADESI-03) and pruritus scores (p < 0.0001) calculated using a pruritus visual analog scale. Transepidermal water loss, a biomarker of skin barrier function, was significantly reduced compared to baseline (day 0) measurements (p = 0.0011). HCA spray was shown to be effective for significantly improving the condition of dogs suffering from CAD. This treatment also significantly improved cut. | Administration, Topical, Animals, Anti-Inflammatory Agents, Dermatitis, Atopic, Dog Diseases, Dogs, Female, Hydrocortisone, Male | null |
22,705,742 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature. | Kim Se Eun, Lee Eui Ri, Lee Yesran, Jeong Manbok, Park Young Woo, Ahn Jae Sang, Ahn Jeong Taek, Seo Kangmoon | eng | null | Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | Analgesics, Opioid, Tramadol | IM | 22705742, 201206193, 10.4142/jvs.2012.13.2.193, PMC3386345, 10807121, 11442735, 17214846, 14121956, 415276, 14158464, 16128833, 19228267, 21086009, 20947064, 1056098, 4272068, 11842933, 3477576, 9509781, 2222256, 15367187, 20593554, 1066091 | This study was designed to assess the effectiveness of a modified silk ligature twisted with wire for inducing advanced periodontitis. Periodontitis was induced in five premolars and one molar of 20 healthy dogs over a 60-day period. The dogs were divided into four groups according to the ligature-inducing materials used: soft moistened food only, wire ligature (WL), silk ligature (SL) and twisted ligature with silk and wire (SWL). Periodontal indices were recorded, and dental radiographs were taken before and after 60 days of ligation. The ligatures were checked daily and the day the ligature fell out was noted. The period during which the ligatures were maintained was significantly shorter for the SL group compared to the SWL group (p < 0.05). Results of the clinical examination showed that almost all periodontal status parameters including the plaque index, gingival index, clinical attachment level, and bleeding on probing were significantly exacerbated in the SWL group compared to the other groups (p < 0.05). Radiographic evaluation demonstrated that alveolar bone levels were significantly lower in the SWL group than the other groups on day 60 (p < 0.05). These results suggested that experimental periodontitis induced by SWL could be an effective method for investigating periodontitis in canine models. | Alveolar Bone Loss, Analgesics, Opioid, Animals, Dog Diseases, Dogs, Ligation, Materials Testing, Pain, Periodontitis, Tramadol | null |
22,705,743 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens. | Zanetti Flavia Adriana, Del Médico Zajac María Paula, Taboga Oscar Alberto, Calamante Gabriela | eng | null | Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Antibodies, Viral, VP2 protein, infectious bursal disease virus, Viral Structural Proteins, Viral Vaccines | IM | 22705743, 201206199, 10.4142/jvs.2012.13.2.199, PMC3386346, 10869768, 16194586, 17987847, 8178574, 21501642, 6285882, 15149796, 8718576, 7000445, 10982331 | A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our results suggest that VP2 protein expressed from MVA-VP2 maintained the correct conformational structure. To our knowledge, this is the first report on the usefulness of MVA-based vectors for developing recombinant vaccines for poultry. | Animals, Antibodies, Viral, Birnaviridae Infections, Cells, Cultured, Chick Embryo, Chickens, Fibroblasts, Infectious bursal disease virus, Poultry Diseases, Specific Pathogen-Free Organisms, Vaccinia virus, Viral Structural Proteins, Viral Vaccines | null |
22,705,744 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV. | Balamurugan Vinayagamurthy, Sen Arnab, Venkatesan Gnanavel, Bhanot Vandana, Yadav Vineeta, Bhanuprakash Veerakyathappa, Singh Raj Kumar | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705744, 201206203, 10.4142/jvs.2012.13.2.203, PMC3386347, 15967242, 20707980, 15062954, 18541325, 15503204, 22105906, 17488738, 8837881, 10523566, 20642492, 15030603, 11742649, 16838207, 3424615 | In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and phylogenetic analyses of the structural genes of the isolated virus confirmed that the virus belonged to Asian lineage IV and was closely related to PPRV circulating in India. | Animals, Cloning, Molecular, Lions, Peste-des-petits-ruminants virus, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction | null |
22,705,745 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Sertoli cell tumors associated with feminizing syndrome and spermatic cord torsion in two cryptorchid dogs. | Quartuccio Marco, Marino Gabriele, Garufi Giuseppe, Cristarella Santo, Zanghì Antonina | eng | null | Case Reports, Journal Article | null | IM | 22705745, 201206207, 10.4142/jvs.2012.13.2.207, PMC3386348, 1162870, 6138340, 3372340, 7340923, 2863879, 4391618, 12201446 | The association of cryptorchidism, functional Sertoli cell tumors, and spermatic cord torsion has been rarely reported in the literature. Two dogs were admitted for bilateral skin alopecia and weight loss. Both animals were cryptorchid and displayed a pendulous preputial sheath, prostate hypertrophy, and increased levels of circulating oestrogen. Transabdominal palpation and ultrasonography revealed the presence of neoplastic retained gonads. During surgery, spermatic cord torsion was also detected in the enlarged neoplastic testes of both dogs. Histologic examination confirmed the presence of Sertoli cell tumors that were primarily responsible for the feminizing syndrome. Complete remission of all symptoms occurred within 3 months after orchiectomy. | Animals, Cryptorchidism, Dog Diseases, Dogs, Male, Sertoli Cell Tumor, Spermatic Cord Torsion, Treatment Outcome | null |
22,705,746 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer. | Kang Jung Taek, Kim Hyung Jin, Oh Hyun Ju, Hong So Gun, Park Jung Eun, Kim Min Jung, Kim Min Kyu, Jang Goo, Kim Dae Yong, Lee Byeong Chun | eng | null | Case Reports, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705746, 201206211, 10.4142/jvs.2012.13.2.211, PMC3386349, 17275081, 16079832, 18577875, 18534672, 15997188, 18524504, 16466782, 2374589, 19287029, 20537823 | Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study, we conducted further clinical, histological, and genetic analyses. This cloned wolf had a normal uterus but developed ovotestis. Through molecular analysis of the SRY gene, a mutation in the coding sequence of SRY gene could be excluded as a cause of intersexuality. This is the first report of a cloned wolf with a 78, XY ovotesticular disorder affecting sexual development characterized by bilateral ovotestes. | Animals, Cloning, Organism, Female, Karyotyping, Mutation, Nuclear Transfer Techniques, Ovotesticular Disorders of Sex Development, Wolves | null |
22,705,747 | 2012-10-22 | 2021-10-21 | 1976-555X | Journal of veterinary science | Ultrasonographic findings of pylorogastric intussusceptions in two dogs. | Choi Jihye, Keh Seoyeon, Kim Taeeun, Jang Jaeyoung, Kim Hyunwook, Yoon Junghee | eng | null | Case Reports, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705747, 201206215, 10.4142/jvs.2012.13.2.215, PMC3386350, 16229433, 17285402, 11361115, 1778746, 12549615 | A Yorkshire terrier (case 1) and a Miniature Schnauzer (case 2) were diagnosed with pylorogastric intussusceptions (PGIs). Both cases showed acute vomiting and had previous histories of laparotomy. In case 1, the invaginated pyloric wall was thickened unevenly containing multiple hypoechoic areas and had indistinct wall layering on ultrasonography. PGI with diffuse gastric edema and necrosis was confirmed on laparotomy. The dog recovered completely after gastrectomy and a Y-U plasty. Case 2 had uniformly thickened walls of invaginated gastric pylorus with the distinct wall layering. PGI was reduced spontaneously the next day. | Animals, Dog Diseases, Dogs, Female, Intussusception, Pylorus, Ultrasonography | null |
22,705,749 | 2013-01-28 | 2016-11-25 | 1873-2941 | Chemistry and physics of lipids | Biophysical properties of ergosterol-enriched lipid rafts in yeast and tools for their study: characterization of ergosterol/phosphatidylcholine membranes with three fluorescent membrane probes. | Bastos André E P, Marinho H Susana, Cordeiro André M, de Soure António M, de Almeida Rodrigo F M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fluorescent Dyes, Phosphatidylcholines, Ergosterol | IM | 22705749, S0009-3084(12)00072-2, 10.1016/j.chemphyslip.2012.06.002 | In this work, binary mixtures of phospholipid/ergosterol (erg) were studied using three fluorescent membrane probes. The phospholipid was either saturated (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) or monounsaturated (1-palmitoyl-2-dioleoyl-sn-glycero-3-phosphocholine, POPC) phosphatidylcholine, to evaluate the fluorescence properties of the probes in gel, liquid ordered (l(o)) and liquid disordered (l(d)) phases. The probes have been used previously to study cholesterol-enriched domains, but their photophysical properties in erg-enriched membranes have not been characterized. N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (NBD-DPPE) presents modest blue-shifts upon erg addition, and the changes in the fluorescence lifetime are mainly due to differences in the efficiency of its fluorescence dynamic self-quenching. However, the steady-state fluorescence anisotropy of NBD-DPPE presents well-defined values in each lipid phase. N-(lissamine rhodamine B sulfonyl)-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (Rhod-DOPE) presents a close to random distribution in erg-rich membranes. There are no appreciable spectral shifts and the steady-state fluorescence anisotropy presents complex behavior, as a result of different photophysical processes. The probe is mostly useful to label l(d) domains in yeast membranes. 4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-pyridinium (di-4-ANEPPS) is an electrochromic dye with excitation spectra largely insensitive to the presence of erg, but presenting a strong blue-shift of its emission with increasing concentrations of this sterol. Its partition coefficient is favorable to l(o) domains in POPC/erg mixtures. Although the fluorescence properties of di-4-ANEPPS are less sensitive to erg than to chol, in both cases the fluorescence lifetime responds monotonically to sterol mole fraction, becoming significantly longer in the presence of sterol as compared to pure POPC or DPPC bilayers. The probe displays a unique sensitivity to sterol-lipid interaction due to the influence of hydration and H-bonding patterns at the membrane/water interface on its fluorescence properties. This makes di-4-ANEPPS (and possibly similar probes) potentially useful in the study of erg-enriched domains in more complex lipid mixtures and in the membranes of living yeast cells. | Biophysical Phenomena, Ergosterol, Fluorescent Dyes, Membrane Microdomains, Phosphatidylcholines, Saccharomyces cerevisiae, Spectrometry, Fluorescence | null |
22,705,748 | 2012-10-25 | 2021-10-21 | 1361-6560 | Physics in medicine and biology | Development of a patient-specific two-compartment anthropomorphic breast phantom. | Prionas Nicolas D, Burkett George W, McKenney Sarah E, Chen Lin, Stern Robin L, Boone John M | eng | R01 EB002138 (NIBIB NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705748, 10.1088/0031-9155/57/13/4293, PMC3404613, NIHMS386860, 22380376, 19175091, 21815391, 21452702, 21397528, 18975714, 15339900, 21626952, 21624698, 21422591, 19673211, 16106024, 21606556, 18195383, 2333054, 21452712, 11339738, 22149826, 19556683, 18491511, 17822008, 20095256, 760167, 21452726 | The purpose of this paper is to develop a technique for the construction of a two-compartment anthropomorphic breast phantom specific to an individual patient's pendant breast anatomy. Three-dimensional breast images were acquired on a prototype dedicated breast computed tomography (bCT) scanner as part of an ongoing IRB-approved clinical trial of bCT. The images from the breast of a patient were segmented into adipose and glandular tissue regions and divided into 1.59 mm thick breast sections to correspond to the thickness of polyethylene stock. A computer-controlled water-jet cutting machine was used to cut the outer breast edge and the internal regions corresponding to glandular tissue from the polyethylene. The stack of polyethylene breast segments was encased in a thermoplastic 'skin' and filled with water. Water-filled spaces modeled glandular tissue structures and the surrounding polyethylene modeled the adipose tissue compartment. Utility of the phantom was demonstrated by inserting 200 µm microcalcifications as well as by measuring point dose deposition during bCT scanning. Affine registration of the original patient images with bCT images of the phantom showed similar tissue distribution. Linear profiles through the registered images demonstrated a mean coefficient of determination (r(2)) between grayscale profiles of 0.881. The exponent of the power law describing the anatomical noise power spectrum was identical in the coronal images of the patient's breast and the phantom. Microcalcifications were visualized in the phantom at bCT scanning. The real-time air kerma rate was measured during bCT scanning and fluctuated with breast anatomy. On average, point dose deposition was 7.1% greater than the mean glandular dose. A technique to generate a two-compartment anthropomorphic breast phantom from bCT images has been demonstrated. The phantom is the first, to our knowledge, to accurately model the uncompressed pendant breast and the glandular tissue distribution for a specific patient. The modular design of the phantom allows for studies of a single breast segment and the entire breast volume. Insertion of other devices, materials and tissues of interest into the phantom provide a robust platform for future breast imaging and dosimetry studies. | Breast, Female, Humans, Mammography, Middle Aged, Phantoms, Imaging, Precision Medicine | null |
22,705,750 | 2012-11-23 | 2015-11-19 | 1872-678X | Journal of neuroscience methods | Introducing Amylo-Glo, a novel fluorescent amyloid specific histochemical tracer especially suited for multiple labeling and large scale quantification studies. | Schmued Larry, Raymick James, Tolleson William, Sarkar Sumit, Zhang Yi-Hong, Bell-Cohn Ashlee | eng | null | Journal Article, Research Support, U.S. Gov't, P.H.S. | Fluorescent Dyes | IM | 22705750, S0165-0270(12)00188-4, 10.1016/j.jneumeth.2012.05.019 | One of the hallmark pathologies associated with Alzheimer's disease (AD) is the conspicuous deposition of extracellular amyloid plaques within the forebrain. These plaques are primarily composed of fibrular aggregates of the A-beta peptide. Traditional methods for the histological localization of these plaques typically rely on the use of the tracers Congo Red or Thioflavin S. This study describes the characterization of a novel fluorescent histochemical probe, Amylo-Glo, for the high resolution and contrast localization of amyloid plaques in brain tissue sections. Potential advantages over conventional amyloid plaque stains such as Congo Red or Thioflavin S can be attributed to its unique chemical and spectral properties. Specifically, it results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination with multiple immunofluorescent labeling studies. | Animals, Brain, Fluorescent Antibody Technique, Fluorescent Dyes, Immunohistochemistry, Mice, Mice, Transgenic, Plaque, Amyloid | null |
22,705,751 | 2014-02-04 | 2018-12-02 | 1878-8769 | World neurosurgery | Intravascular ultrasound: a plus in the management of cerebral venous intravascular problems? | Raftopoulos Christian | eng | null | Journal Article, Comment | null | IM | 22705751, S1878-8750(12)00614-6, 10.1016/j.wneu.2012.06.003 | null | Cerebral Veins, Cerebrovascular Disorders, Female, Humans, Intracranial Hypertension, Male, Ultrasonography, Interventional, Venous Thrombosis | Cerebral venous stenosis, Cerebral venous thrombosis, IVUS, Intravascular ultrasound, Intravascular ultrasound imaging, MRI, Magnetic resonance imaging, Tissue plasminogen activator, Venous flow obstruction, tPA |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.