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22,704,930
2012-10-30
2017-11-16
1879-2448
Water research
Transport of two naphthoic acids and salicylic acid in soil: experimental study and empirical modeling.
Hanna K, Lassabatere L, Bechet B
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Carboxylic Acids, Naphthalenes, Naphthols, Soil, Soil Pollutants, 1-naphthoic acid, Salicylic Acid, 1-hydroxy-2-naphthoic acid
IM
22704930, S0043-1354(12)00292-8, 10.1016/j.watres.2012.04.037
In contrast to the parent compounds, the mechanisms responsible for the transport of natural metabolites of polycyclic aromatic hydrocarbons (PAH) in contaminated soils have been scarcely investigated. In this study, the sorption of three aromatic acids (1-naphthoic acid (NA), 1-hydroxy-2-naphthoic acid (HNA) and salicylic acid (SA)) was examined on soil, in a batch equilibrium single-system, with varying pH and acid concentrations. Continuous flow experiments were also carried out under steady-state water flow. The adsorption behavior of naphthoic and benzoic acids was affected by ligand functionality and molecular structure. All modeling options (equilibrium, chemical nonequilibrium, i.e. chemical kinetics, physical nonequilibrium, i.e. surface sites in the immobile water fraction, and both chemical and physical nonequilibrium) were tested in order to describe the breakthrough behavior of organic compounds in homogeneously packed soil columns. Tracer experiments showed a small fractionation of flow into mobile and immobile compartments, and the related hydrodynamic parameters were used for the modeling of reactive transport. In all cases, the isotherm parameters obtained from column tests differed from those derived from the batch experiments. The best accurate modeling was obtained considering nonequilibrium for the three organic compounds. Both chemical and physical nonequilibrium led to appropriate modeling for HNA and NA, while chemical nonequilibrium was the sole option for SA. SA sorption occurs mainly in mobile water and results from the concomitancy of instantaneous and kinetically limited sites. For all organic compounds, retention is contact condition dependent and differs between batch and column experiments. Such results show that preponderant mechanisms are solute dependent and kinetically limited, which has important implications for the fate and transport of carboxylated aromatic compounds in contaminated soils.
Adsorption, Batch Cell Culture Techniques, Carboxylic Acids, Hydrogen-Ion Concentration, Models, Chemical, Motion, Naphthalenes, Naphthols, Salicylic Acid, Soil, Soil Pollutants, Temperature
null
22,704,932
2012-10-22
2012-07-17
1090-2104
Biochemical and biophysical research communications
Stimulation of Cryptococcus neoformans isolated from skin lesion of AIDS patient matures dendritic cells and promotes HIV-1 trans-infection.
Qin Yan, Li Yu-Ye, Jiang Ai-Ping, Jiang Jin-Feng, Wang Jian-Hua
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704932, S0006-291X(12)01093-5, 10.1016/j.bbrc.2012.06.020
Dendritic cells (DCs) play a pivotal role in host defense against invaded pathogens including fungi, while DCs are targeted by fungi for deleterious regulation of the host immune response. A few studies have reported fungal modulation of DC function in these immunocompromised AIDS patients. Cryptococcus neoformans (C. neoformans) is referred as one of the opportunistic fungi of AIDS. Here, we isolated native C. neoformans from an AIDS patient and investigated its effects on DC activation and function. Stimulation of C. neoformans matured DCs, and enhanced DC-mediated HIV-1 trans-infection; moreover, C. neoformans-stimulated DCs promoted the activation of resting T cells and provided more susceptible targets for HIV-1 infection. Microbial translocation has been proposed as the cause of systemic immune activation in chronic HIV-1 infection. Understanding the potential effects of pathogens on HIV-1-DC interactions could help elucidate viral pathogenesis and provide a new insight for against the spread of HIV.
AIDS-Related Opportunistic Infections, Bacterial Translocation, Cryptococcosis, Cryptococcus neoformans, Dendritic Cells, HEK293 Cells, HIV-1, Humans, Immunocompromised Host, Lymphocyte Activation, Skin, T-Lymphocytes
null
22,704,931
2012-10-22
2022-12-07
1090-2104
Biochemical and biophysical research communications
Plasma amyloid-β oligomers level is a biomarker for Alzheimer's disease diagnosis.
Zhou L, Chan K H, Chu L W, Kwan J S C, Song Y Q, Chen L H, Ho P W L, Cheng O Y, Ho J W M, Lam K S L
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Amyloid beta-Peptides, Biomarkers, Peptide Fragments, amyloid beta-protein (1-40), amyloid beta-protein (1-42)
IM
22704931, S0006-291X(12)01090-X, 10.1016/j.bbrc.2012.06.017
Amyloid beta (Aβ), especially Aβ oligomers, is important in Alzheimer's disease (AD) pathogenesis. We studied plasma Aβ(40), Aβ(42), and Aβ oligomers levels in 44 AD patients and 22 non-demented controls. Cognitive functions were assessed by Chinese version of mini-mental state examination (MMSE), Abbreviated Metal Test (AMT), Alzheimer's Disease Assessment Scale Cognitive Subscale (ADAS-cog). Plasma Aβ monomers and oligomers levels were measured by ELISA. We found that the median plasma Aβ(40) and Aβ(42) levels were similar between AD and controls, and without significant correlation with cognition. Plasma Aβ oligomers level was higher in AD than controls (642.54 ng/ml [range 103.33-2676.93] versus 444.18 ng/ml [range 150.19-1311.18], p=0.047), and negatively correlated with cognition. In multivariate logistic regression analysis, the highest tertile of Aβ oligomers levels showed an increased risk of AD than the combined group of middle and lowest tertiles (OR=8.85, p=0.013), after adjustment of gender, age and APOE4 genotype. Increased plasma Aβ oligomers level was associated with decreased MMSE and AMT scores (p=0.037, p=0.043, respectively) and increased ADAS-cog score (p=0.036), suggesting negative correlation with cognitive function. We concluded that plasma Aβ oligomers level is an useful biomarker for AD diagnosis.
Aged, Aged, 80 and over, Alzheimer Disease, Amyloid beta-Peptides, Asian People, Biomarkers, China, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Peptide Fragments
null
22,704,934
2012-09-20
2012-07-09
1090-2104
Biochemical and biophysical research communications
Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment.
Fujimura Akiko, Kishimoto Hiroyuki, Yanagisawa Junn, Kimura Keiji
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, ERH protein, human, Transcription Factors, centromere protein E
IM
22704934, S0006-291X(12)01091-1, 10.1016/j.bbrc.2012.06.018
The enhancer of rudimentary homolog (ERH) is a small eukaryotic protein that is highly conserved in animals, plants, and protists but not in fungi. ERH has several binding proteins and has been associated with various cellular processes, such as pyrimidine metabolism, cell cycle progression, and transcription control; however, little is known about the exact role of this protein and the underlying molecular mechanisms. We found that ERH has a critical role in the mitotic phase of the cell cycle. ERH depleted-cells showed severe chromosome misalignment and weakened kinetochore-microtubule attachment. ERH depletion also caused dissociation of centromere-associated protein E (CENP-E), a mitotic kinesin that is involved in stabilizing the kinetochore-microtubule attachment, from kinetochores of mitotic chromosomes. We propose that ERH contributes to chromosome alignment at the metaphase plate by localizing CENP-E at kinetochore regions.
Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosomes, Human, HeLa Cells, Humans, Kinetochores, Metaphase, Mitosis, Transcription Factors
null
22,704,933
2012-10-22
2025-01-03
1090-2104
Biochemical and biophysical research communications
Phosphorylation of the transcriptional regulator MYB44 by mitogen activated protein kinase regulates Arabidopsis seed germination.
Nguyen Xuan Canh, Hoang My Hanh Thi, Kim Ho Soo, Lee Kyunghee, Liu Xiao-Min, Kim Sun Ho, Bahk Sunghwa, Park Hyeong Cheol, Chung Woo Sik
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Arabidopsis Proteins, At5g67300 protein, Arabidopsis, Transcription Factors, AtMPK3 protein, Arabidopsis, MPK6 protein, Arabidopsis, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase Kinases
IM
22704933, S0006-291X(12)01092-3, 10.1016/j.bbrc.2012.06.019
The phytohormones abscisic acid (ABA) and gibberellic acid (GA) have antagonistic roles in the control of seed germination and seedling development. We report here that the transcriptional regulator MYB44 has a role in the control of seed germination in Arabidopsis thaliana. High levels of the MYB44 transcript are found in dry seeds but the transcript levels decrease during germination. The decrease in transcript level during germination is inhibited by the GA biosynthesis inhibitor paclobutrazol (PAC). MYB44 is phosphorylated by both recombinant and native forms of MPK3 and MPK6 at Ser(53) and Ser(145). Transgenic overexpression of MYB44 results in increased sensitivity of seed germination to ABA or PAC treatment. The PAC-insensitive germination phenotype of the myb44 mutant is complemented by overexpression of wild type MYB44 but not by overexpression of a mutant protein that lacks the MPK-target serines indicating that phosphorylation of MYB44 by MPKs is required for its biological function.
Arabidopsis, Arabidopsis Proteins, Gene Expression Regulation, Plant, Germination, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases, Phosphorylation, Seeds, Transcription Factors
null
22,704,936
2012-09-20
2012-07-09
1090-2104
Biochemical and biophysical research communications
Novel DNA damage checkpoint in mitosis: Mitotic DNA damage induces re-replication without cell division in various cancer cells.
Hyun Sun-Yi, Rosen Eliot M, Jang Young-Joo
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704936, S0006-291X(12)01111-4, 10.1016/j.bbrc.2012.06.023
DNA damage induces multiple checkpoint pathways to arrest cell cycle progression until damage is repaired. In our previous reports, when DNA damage occurred in prometaphase, cells were accumulated in 4 N-DNA G1 phase, and mitosis-specific kinases were inactivated in dependent on ATM/Chk1 after a short incubation for repair. We investigated whether or not mitotic DNA damage causes cells to skip-over late mitotic periods under prolonged incubation in a time-lapse study. 4 N-DNA-damaged cells re-replicated without cell division and accumulated in 8 N-DNA content, and the activities of apoptotic factors were increased. The inhibition of DNA replication reduced the 8 N-DNA cell population dramatically. Induction of replication without cell division was not observed upon depletion of Chk1 or ATM. Finally, mitotic DNA damage induces mitotic slippage and that cells enter G1 phase with 4 N-DNA content and then DNA replication is occurred to 8 N-DNA content before completion of mitosis in the ATM/Chk1-dependent manner, followed by caspase-dependent apoptosis during long-term repair.
Apoptosis, Cell Division, Cell Line, Tumor, DNA Damage, DNA Replication, G1 Phase Cell Cycle Checkpoints, HeLa Cells, Humans, Mitosis, Neoplasms
null
22,704,935
2012-11-06
2012-07-23
1090-2104
Biochemical and biophysical research communications
A versatile PCR-based tandem epitope tagging system for Streptomyces coelicolor genome.
Kim Ji-Nu, Yi Jeong Sang, Lee Bo-Rahm, Kim Eun-Jung, Kim Min Woo, Song Yoseb, Cho Byung-Kwan, Kim Byung-Gee
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Bacterial Proteins, Epitopes
IM
22704935, S0006-291X(12)01110-2, 10.1016/j.bbrc.2012.06.022
Epitope tagging approaches have been widely used for the analysis of functions, interactions and subcellular distributions of proteins. However, incorporating epitope sequence into protein loci in Streptomyces is time-consuming procedure due to the absence of the versatile tagging methods. Here, we developed a versatile PCR-based tandem epitope tagging tool for the Streptomyces genome engineering. We constructed a series of template plasmids that carry repeated sequence of c-myc epitope, Flp recombinase target (FRT) sites, and apramycin resistance marker to insert epitope tags into any desired spot of the chromosomal loci. A DNA module which includes the tandem epitope-encoding sequence and a selectable marker was amplified by PCR with primers that carry homologous extensions to the last portion and downstream region of the targeted gene. We fused the epitope tags at the 3' region of global transcription factors of Streptomyces coelicolor to test the validity of this system. The proper insertion of the epitope tag was confirmed by PCR and western blot analysis. The recombinants showed the identical phenotype to the wild-type that proved the conservation of in vivo function of the tagged proteins. Finally, the direct binding targets were successfully detected by chromatin immunoprecipitation with the increase in the signal-to-noise ratio. The epitope tagging system describes here would provide wide applications to study the protein functions in S. coelicolor.
Bacterial Proteins, Drug Resistance, Bacterial, Epitopes, Genetic Engineering, Genome, Bacterial, Plasmids, Polymerase Chain Reaction, Streptomyces coelicolor
null
22,704,937
2012-11-29
2022-03-21
1878-5875
The international journal of biochemistry & cell biology
Selective modulation of different GABAA receptor isoforms by diazepam and etomidate in hippocampal neurons.
Seymour Victoria A L, Curmi John P, Howitt Susan M, Casarotto Marco G, Laver Derek R, Tierney M Louise
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Protein Isoforms, Receptors, GABA-A, Diazepam, Etomidate
IM
22704937, S1357-2725(12)00192-6, 10.1016/j.biocel.2012.06.001
Diazepam modulation of native γ2-containing GABA(A) (γGABA(A)) receptors increases channel conductance by facilitating protein interactions involving the γ2-subunit amphipathic (MA) region, which is found in the cytoplasmic loop between transmembrane domains 3 and 4 (Everitt et al., 2009). However, many drugs, predicted to act on different GABA(A) receptor subtypes, increase channel conductance leading us to hypothesize that conductance variation in GABA(A) receptors may be a general property, mediated by protein interactions involving the cytoplasmic MA stretch of amino acids. In this study we have tested this hypothesis by potentiating extrasynaptic GABA(A) currents with etomidate and examining the ability of peptides mimicking either the γ2- or δ-subunit MA region to affect conductance. In inside-out hippocampal patches from newborn rats the general anesthetic etomidate potentiated GABA currents, producing either an increase in open probability and single-channel conductance or an increase in open probability, as described previously (Seymour et al., 2009). In patches displaying high conductance channels application of a δ((392-422)) MA peptide, but not a scrambled version or the equivalent γ2((381-403)) MA peptide, reduced the potentiating effects of etomidate, significantly reducing single-channel conductance. In contrast, when GABA currents were potentiated by the γ2-specific drug diazepam the δ MA peptide had no effect. These data reveal that diazepam and etomidate potentiate different extrasynaptic GABA(A) receptor subtypes but both drugs modulate conductance similarly. One interpretation of the data is that these drugs elicit potentiation through protein interactions and that the MA peptides compete with these interactions to disrupt this process.
Amino Acid Sequence, Animals, Diazepam, Electric Conductivity, Etomidate, Female, Hippocampus, Hydrophobic and Hydrophilic Interactions, Male, Models, Molecular, Molecular Sequence Data, Neurons, Permeability, Protein Isoforms, Protein Structure, Secondary, Rats, Rats, Wistar, Receptors, GABA-A
null
22,704,941
2013-09-04
2021-10-21
1578-1275
Atencion primaria
[Working with our smoker patients in primary care. Analysis of cost-effectiveness].
Castañal-Canto Xulio, Martín-Miguel María Victoria, Hervés-Beloso Cristina, Pérez-Cachafeiro Santiago, Espinosa-Arévalo María Mercedes, Delgado-Martín José Luis
spa
null
English Abstract, Journal Article
null
IM
22704941, S0212-6567(12)00162-X, 10.1016/j.aprim.2012.02.013, PMC7025639, 15213107, 12106550, 12113733, 12495172, 19674514, 14985603, 2740549, 9707823, 12837716, 11578560, 20619509, 15087348, 9470183, 17419933
The aim of this work is to realize an economic evaluation of the smoking interventions in Primary Care (PC).
Cost-Benefit Analysis, Cross-Sectional Studies, Decision Trees, Direct Service Costs, Family Practice, Humans, Primary Health Care, Sensitivity and Specificity, Smoking, Smoking Cessation, Time Factors
null
22,704,942
2012-12-12
2022-03-10
1532-2777
Medical hypotheses
P75 neurotrophin receptor is a regulatory factor in sudden cardiac death with myocardial infarction.
Yuan Ming-Jie, Huang He, Huang Cong-Xin
eng
null
Journal Article
Receptor, Nerve Growth Factor
IM
22704942, S0306-9877(12)00258-7, 10.1016/j.mehy.2012.05.035
Sudden cardiac death (SCD) is a major cause of morbidity and mortality in patients with coronary artery diseases and myocardial infarction (MI). Sympathetic stimulation and sympathetic neural remodeling are important in the generation of SCD in diseased heart. The balance of nerve growth factor (NGF) and semaphoring 3A determines the sympathetic innervation patterning. Recently studies showed that P75 neurotrophin receptor (P75 NTR) is the main receptor for NGF mediates sympathetic hyperinnervation in the heart, and also interacts with semaphoring 3A. Sympathetic axons lacking P75 NTR are more sensitive to semaphoring 3A in vitro than control neurons, resulting in decreased sympathetic innervation in the left ventricular subendocardium. P75 NTR(-/-) mice had increased sympathetic heterogeneity and more spontaneous ventricular arrhythmias. Based on current studies, we present a hypothesis that P75 NTR plays an important regulatory role in sudden cardiac after myocardial infarction and hope to find new therapeutic target for SCD.
Death, Sudden, Cardiac, Humans, Models, Theoretical, Myocardial Infarction, Receptor, Nerve Growth Factor
null
22,704,943
2012-12-12
2012-08-08
1532-2777
Medical hypotheses
Hygiene hypothesis: why south/north geographical differences in prevalence of asthma and sarcoidosis?
Kurata Atsushi
eng
null
Journal Article
null
IM
22704943, S0306-9877(12)00259-9, 10.1016/j.mehy.2012.05.036
Although asthma is multi-factorial and generally worsens during winter, prevalence of asthma tends to be higher in warm regions. By contrast, sarcoidosis, which like asthma results from immunological abnormalities that disturb the lower respiratory tracts and unlike asthma is characterized by T helper (Th) 1 response, occurs predominantly in colder regions. The hygiene hypothesis proposes that infection by intracellular pathogens such as early childhood viruses promotes Th1 immune phenotype and decreases susceptibility to later occurrence of Th2-associated asthma, since Th1 and Th2 cells are mutually inhibitory. As respiratory viral infections are generally more common under colder conditions, it is hypothesized that more respiratory viral infections in colder climates than one's natural environment during early childhood may promote subsequent occurrence of sarcoidosis, while less infections in warmer environments may promote subsequent occurrence of asthma.
Asthma, Geography, Humans, Models, Theoretical, Prevalence, Sarcoidosis
null
22,704,944
2013-03-05
2022-03-09
1476-5411
Contact lens & anterior eye : the journal of the British Contact Lens Association
Changes in central corneal thickness values after instillation of oxybuprocaine hydrochloride 0.4%.
Ogbuehi Kelechi C, Chijuka John C, Osuagwu Uchechukwu L
eng
null
Journal Article, Randomized Controlled Trial
Anesthetics, Local, Procaine, benoxinate
IM
22704944, S1367-0484(12)00057-4, 10.1016/j.clae.2012.05.004
To assess the variation in central corneal thickness (CCT) following the instillation of oxybuprocaine hydrochloride (0.4%), in normal subjects.
Adult, Anesthetics, Local, Cornea, Corneal Topography, Female, Humans, Male, Microscopy, Ophthalmoscopy, Organ Size, Procaine, Reproducibility of Results, Sensitivity and Specificity, Young Adult
null
22,704,939
2015-06-22
2015-11-19
2340-3284
Revista espanola de anestesiologia y reanimacion
[Miller-Fisher variant of Guillain-Barré syndrome in the Resuscitation Unit].
López Erausquin N, Aguilera Celorrio L
spa
null
Case Reports, Letter
Immunoglobulins, Intravenous, Influenza Vaccines
IM
22704939, S0034-9356(12)00177-6, 10.1016/j.redar.2012.05.003
null
Aged, Combined Modality Therapy, Comorbidity, Critical Care, Female, Humans, Immunoglobulins, Intravenous, Influenza Vaccines, Intensive Care Units, Intubation, Intratracheal, Miller Fisher Syndrome, Plasmapheresis, Respiration, Artificial
null
22,704,938
2015-11-05
2012-08-06
2340-3284
Revista espanola de anestesiologia y reanimacion
[Use of the McGrath® video laryngoscope and paediatric tube exchanger for endotrachial tube replacement in a patient with a difficult airway].
Ramos Costoya J, Añez Simón C, Santos Marqués M L
spa
null
Case Reports, Letter
null
IM
22704938, S0034-9356(12)00178-8, 10.1016/j.redar.2012.05.004
null
Accidents, Traffic, Airway Management, Anthropometry, Bronchoscopy, Fiber Optic Technology, Fracture Fixation, Internal, Humans, Intubation, Intratracheal, Laryngoscopes, Male, Middle Aged, Skull Fractures, Video Recording
null
22,704,946
2012-11-20
2022-03-16
1532-2653
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia
Correlation of leukocytosis with early neurological deterioration following supratentorial intracerebral hemorrhage.
Sun Wei, Peacock Amanda, Becker Jane, Phillips-Bute Barbara, Laskowitz Daniel T, James Michael L
eng
D43 TW008303 (FIC NIH HHS, United States); D43 TW008308 (FIC NIH HHS, United States); L30 NS071908 (NINDS NIH HHS, United States); D43-TW008308-01 (FIC NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
null
IM
22704946, S0967-5868(12)00023-9, 10.1016/j.jocn.2011.11.020, PMC3706635, NIHMS387789, 12383357, 21387381, 20692165, 15304576, 17478736, 16905751, 18830379, 8711791, 19828550, 11283388, 8058133, 21057958, 17502545, 19109539, 19910545, 12805488, 17379820, 20934153, 20948399, 19803787, 8996478, 18556582, 19505208, 3975963, 19251191, 9390653, 19240605
Intracerebral hemorrhage (ICH) is a devastating and common admitting diagnosis to intensive care units in the USA. Despite advances in critical care, patients with ICH often experience early neurological deterioration (END) in the first 72 hours after admission due to a variety of factors, including hematoma and cerebral edema evolution. The purpose of this study was to determine factors associated with END after ICH. Using the Duke University Hospital Neuroscience Critical Care Unit Database, we retrospectively identified patients with an admitting diagnosis of supratentorial ICH from January to December 2010, verified by CT imaging. END was defined as a decrease in the Glasgow Coma Scale score of ≥3 or death within the first 72 hours after hemorrhage. The chi-squared or t-test analysis was used to compare the groups, as appropriate. Multiple logistical regression modeling was performed to test for associations between likely predictors of END. Of the 89 subjects admitted with supratentorial ICH, we included 83 in the analysis based on complete datasets. Of these, 31 experienced END within 72 hours after onset of symptoms. ICH score, presence of midline shift on imaging, and white blood cell (WBC) count were used in a regression model for predicting END. WBC count demonstrated the greatest association with END. Patients with ICH are prone to END within the first few days after hemorrhage. Elevated WBC count appears predictive of deterioration. These data demonstrate that heightened inflammatory state after ICH may be related to early deterioration after injury.
Adult, Aged, Aged, 80 and over, Analysis of Variance, Cerebral Hemorrhage, Female, Glasgow Coma Scale, Humans, Leukocyte Count, Leukocytosis, Male, Middle Aged, Nervous System Diseases, Statistics as Topic, Tomography, X-Ray Computed
null
22,704,945
2012-11-20
2018-12-01
1532-2653
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia
Systematic review of primary intracranial glioblastoma multiforme with symptomatic spinal metastases, with two illustrative patients.
Shahideh Mehdi, Fallah Aria, Munoz David G, Loch Macdonald R
eng
null
Case Reports, Journal Article, Review, Systematic Review
null
IM
22704945, S0967-5868(11)00654-0, 10.1016/j.jocn.2011.09.024
Glioblastoma multiforme is a malignant tumour with a universally fatal diagnosis. We report two patients with glioblastoma with symptomatic metastasis to the spinal cord and perform a systematic review all 35 reports of symptomatic glioblastoma dissemination to the spinal leptomeninges and/or intramedullary spinal axis. Our analysis of the data shows a median time to spinal metastasis of 10 months and a median time of three months from spinal metastasis to death. Treatments described include palliative laminectomies, radiotherapy and chemotherapy. No treatment strategy offered a therapeutic advantage as patients deteriorated rapidly regardless of intervention. Patients who underwent only a biopsy for intracranial glioblastoma had a shorter time to development of spinal metastasis. In addition, there may be an association between intramedullary metastasis and shorter survival. This paper highlights the importance of considering symptomatic spinal dissemination in glioblastoma multiforme. We also review the incidence and postulate mechanisms of tumour dissemination in the central nervous system. Clearly, further research into radiotherapeutic and chemotherapeutic options in this clinical setting is required.
Adult, Brain Neoplasms, Female, Glioblastoma, Humans, Kaplan-Meier Estimate, Magnetic Resonance Imaging, Male, Middle Aged, Spinal Cord Neoplasms, Tomography, X-Ray Computed
null
22,704,947
2013-03-07
2012-10-05
1532-2653
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia
A comparison of the hemodynamic effects of flow diverters on wide-necked and narrow-necked cerebral aneurysms.
Wu Yong-Fa, Yang Peng-Fei, Shen Jie, Huang Qing-Hai, Zhang Xing, Qian Yi, Liu Jian-Min
eng
null
Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704947, S0967-5868(12)00018-5, 10.1016/j.jocn.2011.10.015
Flow diverters (FD), a new generation of intracranial stents with a low porosity mesh, have been applied as an alternative treatment for intracranial aneurysms. However, their efficacy varies among aneurysms of different morphology. In this study, computational fluid dynamic simulations were performed to examine the influence of an FD on the hemodynamics of wide-necked and narrow-necked cerebral aneurysms. An FD with 70% porosity mesh was deployed across the neck of an ideal narrow-necked and wide-neck aneurysm model. The hemodynamics at the aneurysmal sac were changed markedly in both models. At the inflow portion of the aneurysm neck of the narrow-necked aneurysm, the peak velocity and wall shear stress were reduced by 84% and 91%, respectively. By comparison, in the wide-necked aneurysm model, the results were 47% and 21%, respectively. This study demonstrates that the FD markedly altered the hemodynamic conditions inside intracranial aneurysms, depending on aneurysm morphology. Therefore, hemodynamic modifications should be individually designed for aneurysms with different morphology.
Cerebrovascular Circulation, Computer Simulation, Equipment Design, Hemodynamics, Humans, Intracranial Aneurysm, Models, Anatomic, Shear Strength, Stents
null
22,704,948
2012-11-20
2022-04-08
1532-2653
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia
Surgical management of intradural extramedullary tumors located anteriorly to the spinal cord.
Joaquim Andrei Fernandes, Almeida João Paulo, Dos Santos Marcos Juliano, Ghizoni Enrico, de Oliveira Evandro, Tedeschi Helder
eng
null
Case Reports, Journal Article
null
IM
22704948, S0967-5868(12)00037-9, 10.1016/j.jocn.2011.08.044
Meningiomas and nerve sheath tumors are the most common lesions found in the intradural extramedullary compartment of the spine. Some of these lesions can be located anteriorly to the spinal cord, constituting a challenge for spine surgeons. We present a surgical technique that improves the surgical exposure of lesions located anteriorly or antero-laterally to the spinal cord. A microsurgical technique of tenting of the dentate ligament with sutures and rotation of the spinal cord is described in detail and illustrated with surgical cases. This technique increases the small microsurgical operative field and allows spinal cord retraction with the use of a natural cord component, minimizing pressure on the spinal cord delicate tissue, allowing total tumor resection. In conclusion, total resection without new neurological deficit of anterior and antero-lateral tumors can be performed using an isolated posterior approach with rotation of the spinal cord using tenting of the dentate ligament with sutures.
Adult, Female, Humans, Laminectomy, Magnetic Resonance Imaging, Male, Meningeal Neoplasms, Meningioma, Middle Aged, Neurosurgical Procedures, Spinal Cord, Young Adult
null
22,704,949
2012-10-16
2015-11-19
1095-6859
Gynecologic oncology
Laparoscopic lymph node dissection should be performed before fertility preserving treatment of patients with cervical cancer.
Vercellino Giuseppe F, Piek Jurgen M J, Schneider Achim, Köhler Christhardt, Mangler Mandy, Speiser Dorothee, Chiantera Vito
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Paclitaxel, Cisplatin, Ifosfamide
IM
22704949, S0090-8258(12)00406-4, 10.1016/j.ygyno.2012.05.033
The aim of this study is to assess our results of treatment of women with stage I cervical cancer>2 cm in diameter seeking fertility preservation. Treatment consisted of Laparoscopic Pelvic and Paraaortic Lymphadenectomy (LPPLND), and when no nodal metastasis was detected, neoadjuvant chemotherapy (NACT) followed by radical vaginal trachelectomy (RVT). Patients with positive lymph nodes underwent primary chemoradiation.
Adenocarcinoma, Adult, Antineoplastic Combined Chemotherapy Protocols, Brachytherapy, Carcinoma, Squamous Cell, Cervix Uteri, Chemoradiotherapy, Chemotherapy, Adjuvant, Cisplatin, Female, Fertility Preservation, Follow-Up Studies, Humans, Ifosfamide, Laparoscopy, Lymph Node Excision, Lymphatic Metastasis, Neoadjuvant Therapy, Neoplasm Recurrence, Local, Neoplasm Staging, Paclitaxel, Pelvis, Prospective Studies, Uterine Cervical Neoplasms
null
22,704,950
2013-02-06
2015-11-19
1095-6859
Gynecologic oncology
Health related quality of life and symptoms after pelvic lymphadenectomy or radiotherapy vs. no adjuvant regional treatment in early-stage endometrial carcinoma: a large population-based study.
van de Poll-Franse Lonneke V, Pijnenborg Johanna M A, Boll Dorry, Vos M Caroline, van den Berg Hetty, Lybeert Marnix L M, de Winter Karin, Kruitwagen Roy F P M
eng
null
Journal Article, Multicenter Study, Randomized Controlled Trial, Research Support, Non-U.S. Gov't
null
IM
22704950, S0090-8258(12)00445-3, 10.1016/j.ygyno.2012.06.007
Routine lymphadenectomy (LA) in early stage endometrial cancer does not improve survival. However, in the absence of lymph node metastasis, radiotherapy (RT) could be withheld and hence could result in less morbidity. Our aim was to evaluate health related quality of life (HRQL) in endometrial cancer survivors that received routine pelvic LA without RT compared to no LA, but RT in the presence of risk factors.
Aged, Brachytherapy, Endometrial Neoplasms, Female, Humans, Lymph Node Excision, Middle Aged, Neoplasm Staging, Pelvis, Quality of Life, Radiotherapy, Adjuvant, Risk Factors, Surveys and Questionnaires
null
22,704,951
2013-02-06
2012-09-03
1095-6859
Gynecologic oncology
Impact of the new FIGO 2009 staging classification for vulvar cancer on prognosis and stage distribution.
Tabbaa Zaid M, Gonzalez Jesus, Sznurkowski Jacek J, Weaver Amy L, Mariani Andrea, Cliby William A
eng
null
Journal Article, Multicenter Study
null
IM
22704951, S0090-8258(12)00443-X, 10.1016/j.ygyno.2012.06.005
In 2009, FIGO modified staging of vulvar cancer--the performance of the new classification relative to the prior system has not been assessed. We sought to investigate the impact of the 2009 FIGO vulvar cancer staging system on stage distribution and prognostic ability of the 2009 sub-stage classifications in a large cohort of uniformly staged cases with long-term followup.
Cohort Studies, Female, Humans, Neoplasm Staging, Prognosis, Retrospective Studies, Survival Analysis, Vulvar Neoplasms
null
22,704,953
2013-04-02
2022-03-30
1532-1932
Best practice & research. Clinical obstetrics & gynaecology
Management of male-factor infertility.
Tournaye Herman J, Cohlen Ben J
eng
null
Journal Article
null
IM
22704953, S1521-6934(12)00091-0, 10.1016/j.bpobgyn.2012.05.005
For many years, the management of male-factor infertility has been empirical rather than evidence-based. In current clinical practice, assisted reproductive techniques are the most successful methods of alleviating male-factor infertility. To date, it remains unclear what adjuvant actions can be taken to improve the outcome of assisted reproductive techniques for male-factor infertility. Evidence shows that smoking adversely affects sperm quality to some extent, and the genetic make-up of sperm to a greater extent; however, because of the scarcity and heterogeneity of studies, its effect on in-vitro fertilisation outcome remains largely unknown. Although smoking cessation should be part of the assisted reproductive techniques treatment plan, the benefit of antioxidant treatment in either smokers or non-smokers undergoing assisted reproductive techniques is still under scrutiny. Other lifestyle modifications in subfertile men, such as refraining from moderate alcohol and caffeine consumption, are even more controversial. When embarking on assisted reproductive techniques to alleviate male-factor infertility, intrauterine insemination may be considered as a first-line treatment for couples in whom the female partner has a normal fertility status, and at least 0.8 × 10(6) progressively motile spermatozoa are recovered after sperm preparation. If no pregnancy is achieved after three to six cycles of intrauterine insemination, in-vitro fertilisation can be proposed. When too few progressively motile spermatozoa are obtained after sperm processing for in-vitro fertilisation, or when surgically retrieved sperm are to be used, intracytoplasmic sperm injection is preferable. Although the outcome of no other assisted reproductive techniques has been scrutinised so much, and no large-scale 'macro-problems' have yet been observed after intracytoplasmic sperm injection, malformation rates are reported to be higher compared with the general population. Therefore, candidates for intracytoplasmic sperm injection should be rigorously screened before embarking on in-vitro fertilisation or intracytoplasmic sperm injection, and thoroughly informed of the limitations of our knowledge on the hereditary aspects of male infertility and the safety aspects of assisted reproductive techniques.
Azoospermia, Evidence-Based Medicine, Fertilization in Vitro, Humans, Infertility, Male, Insemination, Artificial, Life Style, Male, Reproductive Techniques, Assisted, Smoking Cessation, Sperm Retrieval, Varicocele
null
22,704,952
2013-03-25
2012-10-01
1873-6807
Body image
Exploring the utility of emotional awareness and negative affect in predicting body satisfaction and body distortion.
Manjrekar Eishita, Berenbaum Howard
eng
null
Journal Article
null
IM
22704952, S1740-1445(12)00072-1, 10.1016/j.bodyim.2012.05.005
In a sample of 304 female college students, the present study examined how body image is associated with (a) clarity of emotion; (b) attention to emotion; and (c) negative affect. Two separate facets of body image were examined: body satisfaction and body distortion. Greater clarity of emotion was associated with greater body satisfaction and less body distortion, and these associations could not be accounted for by negative affect. Body satisfaction was significantly predicted by the three-way interaction of clarity of emotion, attention to emotion, and negative affect. Attention to emotion moderated the association between clarity of emotion and body satisfaction only among high negative affect individuals. Specifically, greater clarity of emotion was associated with greater body satisfaction in all participants except those who were high in both negative affect and attention to emotion.
Adolescent, Affective Symptoms, Attention, Awareness, Body Dysmorphic Disorders, Body Image, Emotions, Feedback, Psychological, Female, Humans, Perceptual Distortion, Personal Satisfaction, Risk Factors, Students, Young Adult
null
22,704,954
2013-07-30
2016-11-26
0006-3002
Biochimica et biophysica acta
Thyroid hormone receptors: the challenge of elucidating isotype-specific functions and cell-specific response.
Flamant Frédéric, Gauthier Karine
eng
null
Journal Article, Review
Protein Isoforms, Receptors, Thyroid Hormone, Thyroid Hormones
IM
22704954, S0304-4165(12)00170-5, 10.1016/j.bbagen.2012.06.003
Thyroid hormone receptors TRα1, TRβ1 and TRβ2 are broadly expressed and exert a pleiotropic influence on many developmental and homeostatic processes. Extensive genetic studies in mice precisely defined their respective function.
Animals, Humans, Protein Isoforms, Receptors, Thyroid Hormone, Signal Transduction, Thyroid Hormones
null
22,704,955
2013-05-24
2021-10-21
1879-1506
Archives of oral biology
Identification and characterization of neural crest-derived cells in adult periodontal ligament of mice.
Kaku Masaru, Komatsu Yoshihiro, Mochida Yoshiyuki, Yamauchi Mitsuo, Mishina Yuji, Ko Ching-Chang
eng
R21 AR057451 (NIAMS NIH HHS, United States); Z01 ES071003 (Intramural NIH HHS, United States); K99 DE021054 (NIDCR NIH HHS, United States); R01 DE019527 (NIDCR NIH HHS, United States); K08DE018695 (NIDCR NIH HHS, United States); R01DE020843 (NIDCR NIH HHS, United States); R01 DE020843 (NIDCR NIH HHS, United States); K08 DE018695 (NIDCR NIH HHS, United States); K99DE021054 (NIDCR NIH HHS, United States); ES071003-11 (NIEHS NIH HHS, United States); DE019527 (NIDCR NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't
null
IM
22704955, S0003-9969(12)00146-X, 10.1016/j.archoralbio.2012.04.022, PMC3537832, NIHMS387295, 19161980, 16186259, 16192680, 2387234, 17158956, 10725243, 9916792, 14581408, 10686619, 12147742, 19415111, 16888282, 6297757, 17459343, 15070746, 15246727, 12674330, 12812797, 8660858, 19576204, 15613081, 12941628, 12716973, 16827724, 15366003, 22020075, 11087820, 17286603, 10419695, 9843687, 11105057, 18159238, 18624954, 9362543, 17651131, 17183711, 15517002, 12194866, 16684817, 16186328, 15649466, 19530172
Cells derived from the neural crest (NC) contribute to the development of several adult tissues, including tooth and periodontal tissue. Here, two transgenic lines, Wnt1-Cre/ZEG and P0-Cre/ZEG, were analysed to determine the fate and distribution of neural crest cells (NCCs) in adult mouse periodontal ligament (PDL).
Animals, Cell Differentiation, Cell Separation, Immunohistochemistry, Mice, Mice, Transgenic, Multipotent Stem Cells, Neural Crest, Periodontal Ligament
null
22,704,957
2013-02-05
2012-09-26
1878-5832
Drug discovery today
The status of the art of human malignant glioma management: the promising role of targeting tumor-initiating cells.
Florio Tullio, Barbieri Federica
eng
null
Journal Article, Research Support, Non-U.S. Gov't, Review
null
IM
22704957, S1359-6446(12)00194-8, 10.1016/j.drudis.2012.06.001
Glioblastoma is the most prevalent and malignant form of brain cancer, but the current available multimodality treatments yield poor survival improvement. Thus, innovative therapeutic strategies represent the challenging topic for glioblastoma management. Multidisciplinary advances, supporting current standard of care therapies and investigational trials that reveal potential drug targets for glioblastoma are reviewed. A radical change in glioblastoma therapeutic approaches could arise from the identification of cancer stem cells, putative tumor-initiating cells involved in tumor initiation, progression and resistance, as innovative drug target. Still controversial identification of markers and molecular regulators in glioma tumor-initiating cells and novel approaches targeting these cells are discussed.
Animals, Brain Neoplasms, Glioblastoma, Humans, Neoplastic Stem Cells
null
22,704,958
2012-10-15
2021-12-03
1873-2968
Biochemical pharmacology
Down-regulation of the PTTG1 proto-oncogene contributes to the melanoma suppressive effects of the cyclin-dependent kinase inhibitor PHA-848125.
Caporali Simona, Alvino Ester, Levati Lauretta, Esposito Alessia I, Ciomei Marina, Brasca Maria G, Del Bufalo Donatella, Desideri Marianna, Bonmassar Enzo, Pfeffer Ulrich, D'Atri Stefania
eng
null
Journal Article, Research Support, Non-U.S. Gov't
MAS1 protein, human, N,1,4,4-tetramethyl-8-((4-(4-methylpiperazin-1-yl)phenyl)amino)-4,5-dihydro-1H-pyrazolo(4,3-h)quinazoline-3-carboxamide, Neoplasm Proteins, Protein Kinase Inhibitors, Proto-Oncogene Mas, Pyrazoles, Quinazolines, RNA, Small Interfering, Securin, pituitary tumor-transforming protein 1, human, Cyclin-Dependent Kinases
IM
22704958, S0006-2952(12)00402-9, 10.1016/j.bcp.2012.06.004
We previously demonstrated that PHA-848125, a cyclin-dependent kinase inhibitor presently under Phase II clinical investigation, impairs melanoma cell growth. In this study, gene expression profiling showed that PHA-848125 significantly modulated the expression of 128 genes, predominantly involved in cell cycle control, in the highly drug-sensitive GL-Mel (p53 wild-type) melanoma cells. Up-regulation of 4 selected genes (PDCD4, SESN2, DDIT4, DEPDC6), and down-regulation of 6 selected genes (PTTG1, CDC25A, AURKA, AURKB, PLK1, BIRC5) was confirmed at protein levels. The same protein analysis performed in PHA-848125-treated M10 melanoma cells - p53 mutated and less sensitive to the drug than GL-Mel cells - revealed no DEPDC6 expression and no changes of PTTG1, PDCD4 and BIRC5 levels. Upon PHA-848125 treatment, a marked PTTG1 down-modulation was also observed in A375 cells (p53 wild-type) but not in CN-Mel cells (p53 mutated). PTTG1 silencing significantly inhibited melanoma cell proliferation and induced senescence, with effects less pronounced in p53 mutated cells. PTTG1 silencing increased PHA-848125 sensitivity of p53 mutated cells but not that of A375 or GL-Mel cells. Accordingly, in M10 but not in A375 cells a higher level of senescence was detected in PHA-848125-treated/PTTG1-silenced cells with respect to PHA-848125-treated controls. In A375 and GL-Mel cells, TP53 silencing attenuated PHA-848125-induced down-modulation of PTTG1 and decreased cell sensitivity to the drug. These findings indicate that PHA-848125-induced down-regulation of PTTG1 depends, at least in part, on p53 function and contributes to the antiproliferative activity of the drug. Our study provides further molecular insight into the antitumor mechanism of PHA-848125.
Cell Division, Cell Line, Tumor, Cyclin-Dependent Kinases, Down-Regulation, Gene Regulatory Networks, Humans, Melanoma, Neoplasm Proteins, Protein Kinase Inhibitors, Proto-Oncogene Mas, Proto-Oncogenes, Pyrazoles, Quinazolines, RNA, Small Interfering, Securin
null
22,704,956
2013-02-05
2021-10-21
1878-5832
Drug discovery today
Recent progress in structure-based anti-influenza drug design.
Du Juan, Cross Timothy A, Zhou Huan-Xiang
eng
R01 AI023007 (NIAID NIH HHS, United States); R01 GM058187 (NIGMS NIH HHS, United States); AI23007 (NIAID NIH HHS, United States); GM58187 (NIGMS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Review
Antiviral Agents, Viral Proteins
IM
22704956, S1359-6446(12)00195-X, 10.1016/j.drudis.2012.06.002, PMC3459301, NIHMS387332, 18660801, 16208317, 19557158, 19587036, 16251290, 19469531, 18235503, 3046255, 21894258, 19428595, 8457561, 16571812, 18454157, 978183, 19461581, 11090151, 12183461, 16456087, 20452227, 21930946, 10930642, 18796704, 16189083, 20824086, 17151603, 19914748, 21625478, 1913813, 10725408, 16424859, 19251591, 11726970, 19144639, 19407738, 18476738, 16543414, 11907320, 8502295, 19651904, 20463064, 17384070, 18725644, 20512121, 12970177, 21691418, 11527739, 19419201, 10774473, 20849817, 19234466, 498272, 19428126, 21466220, 7844831, 1438172, 20966252, 14764886, 17494067, 19052087, 18615018, 8619568, 19331731, 21798894, 16915235, 20427241, 11562490, 9164466, 20394375, 12438632, 9721235, 16526129, 18558668, 22482709, 20015013, 11000002, 18596815, 17539643, 18614582, 19656699, 17081640, 11162800, 19004788, 17310249, 17570112, 18701586, 21094565, 18235504, 20130653, 21819109, 18317572, 20974907, 20494579, 10966468, 19117662, 18049471, 21896751, 15784624, 11897583, 18847186, 18813227, 21744829, 20970464, 21750542, 21081911, 16632600, 19905033, 21566186, 11170976, 20383144, 7038875, 2024485, 15006410, 21237476, 17201676
Seasonal and pandemic influenza have caused high morbidity and mortality worldwide. Recent emergence of influenza A H5N1 and H1N1 strains has heightened concern, especially as a result of their drug resistance. The life cycle of influenza viruses has been well studied and nearly all the viral proteins are becoming potential therapeutic targets. In this review, we present an overview of recent progress in structure-based anti-influenza drug design, paying close attention to the increasing role of computation and strategies for overcoming drug resistance.
Antiviral Agents, Drug Design, Humans, Influenza, Human, Molecular Structure, Viral Proteins
null
22,704,959
2013-05-31
2015-11-19
1873-2364
Neuromuscular disorders : NMD
Brody syndrome: a clinically heterogeneous entity distinct from Brody disease: a review of literature and a cross-sectional clinical study in 17 patients.
Voermans N C, Laan A E, Oosterhof A, van Kuppevelt T H, Drost G, Lammens M, Kamsteeg E J, Scotton C, Gualandi F, Guglielmi V, van den Heuvel L, Vattemi G, van Engelen B G
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Sarcoplasmic Reticulum Calcium-Transporting ATPases
IM
22704959, S0960-8966(12)00116-2, 10.1016/j.nmd.2012.03.012
Brody disease is a rare inherited myopathy due to reduced sarcoplasmic reticulum Ca(2+) ATPase (SERCA)1 activity caused by mutations in ATP2A1, which causes delayed muscle relaxation and silent cramps. So far the disease has mostly been diagnosed by measurement of SERCA1 activity. Since mutation analysis became more widely available, it has appeared that not all patients with reduced SERCA1 activity indeed have ATP2A1 mutations, and a distinction between Brody disease (with ATP2A1 mutations) and Brody syndrome (without ATP2A1 mutations) was proposed. We aim to compare the clinical features of patients with Brody disease and those with Brody syndrome and detect clinical features which help to distinguish between the two. In addition, we describe the Brody syndrome phenotype in more detail. We therefore performed a literature review on clinical features of both Brody disease and Brody syndrome and a cross-sectional clinical study consisting of questionnaires, physical examination, and a review of medical files in 17 Brody syndrome patients in our centre. The results showed that Brody disease presents with an onset in the 1st decade, a generalized pattern of muscle stiffness, delayed muscle relaxation after repetitive contraction on physical examination, and autosomal recessive inheritance. Patients with Brody syndrome more often report myalgia and experience a considerable impact on daily life. Future research should focus on the possible mechanisms of reduction of SERCA activity in Brody syndrome and other genetic causes, and on evaluation of treatment options.
Adolescent, Adult, Aged, Child, Cross-Sectional Studies, DNA Mutational Analysis, Female, Humans, Male, Middle Aged, Mutation, Myotonia Congenita, Phenotype, Review Literature as Topic, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Surveys and Questionnaires, Young Adult
null
22,704,960
2013-01-29
2022-03-18
1873-4138
Meat science
Effects of two transport systems on lamb welfare and meat quality.
Miranda-de la Lama G C, Salazar-Sotelo M I, Pérez-Linares C, Figueroa-Saavedra F, Villarroel M, Sañudo C, Maria G A
eng
null
Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't
Biomarkers
IM
22704960, S0309-1740(12)00198-2, 10.1016/j.meatsci.2012.05.026
The aim of this study was to analyse the effect of a direct transport system (DTS) versus transport with a logistic stopover system (TLS) on lamb welfare and meat quality at two seasons. A total of 96 lambs were sampled in a 2×2×2 factorial design, testing two transport systems and two seasons (summer and winter), with two replicates in each season. Significant interactions (P≤0.05) between transport system and season in both welfare and meat quality were found. In general, lambs subjected to direct transport and logistic stopover during winter had a more intense stress response and poorer meat quality than lambs transported during summer. However, direct transport during the cold season seemed to be the most stressful, compared to the rest of the groups, which was reflected in significantly higher levels of cortisol, lactate, glucose, ratio of N/L, higher pH24 and darker and tougher meat.
Animal Husbandry, Animals, Animals, Inbred Strains, Biomarkers, Cold Temperature, Food Quality, Hydrogen-Ion Concentration, Male, Meat, Mechanical Phenomena, Pigmentation, Seasons, Severity of Illness Index, Sheep, Sheep Diseases, Sheep, Domestic, Spain, Stress, Physiological, Stress, Psychological, Transportation
null
22,704,962
2013-02-28
2022-03-21
1095-9157
Journal of autoimmunity
Rheumatoid arthritis: from autoimmunity to synovitis and joint destruction.
Boissier Marie-Christophe, Semerano Luca, Challal Salima, Saidenberg-Kermanac'h Nathalie, Falgarone Géraldine
eng
null
Journal Article, Review
Antirheumatic Agents, Autoantibodies, Cytokines, Rheumatoid Factor
IM
22704962, S0896-8411(12)00088-1, 10.1016/j.jaut.2012.05.021
Rheumatoid arthritis is an autoimmune disease characterized by the production of two known antibodies - rheumatoid factor and anti-citrullinated peptide antibody (ACPA) - against common autoantigens that are widely expressed within and outside the joints. The interactions between genes and environment are crucial in all stages of the disease, involving namely genes from major histocompatibility complex locus, and antigens such as tobacco or microbes (e.g. Porphyromonas gingivalis). T and B cells are activated as soon as the earliest phases of the disease, rheumatoid arthritis appearing as a Th1 and Th17 disease. Inflammatory cytokines have a considerable importance in the hierarchy of the processes involved in RA. The joint destruction seen in RA is caused not only by cytokine imbalances, but also by specific effects of the Wnt system and osteoprotegerin on osteoclasts and by matrix production dysregulation responsible for cartilage damage. Both innate and adaptative immunity demonstrated their respective cornerstone position in rheumatoid arthritis, since targeted treatments has been efficiently developed against TNF-α, IL-6 receptor, IL-1β, CD20 B cells and T-cell/Dendritic cell interactions.
Antirheumatic Agents, Arthritis, Rheumatoid, Autoantibodies, Autoimmunity, B-Lymphocytes, Cytokines, Humans, Joints, Lymphocyte Activation, Osteoclasts, Rheumatoid Factor, Synovitis, Th1 Cells, Th17 Cells
null
22,704,961
2013-02-28
2024-03-22
1095-9157
Journal of autoimmunity
T cells, murine chronic graft-versus-host disease and autoimmunity.
Eisenberg Robert A, Via Charles S
eng
R01 AI063626 (NIAID NIH HHS, United States); R01 AR034156 (NIAMS NIH HHS, United States)
Journal Article, Review
Autoantibodies, Histocompatibility Antigens Class II
IM
22704961, S0896-8411(12)00084-4, 10.1016/j.jaut.2012.05.017, PMC3578438, NIHMS387781, 11544103, 21115734, 12857969, 2957440, 2968502, 6411402, 22525889, 15944260, 8473754, 9510164, 14634088, 10995792, 2148286, 9916752, 7704000, 12515811, 18219309, 2033370, 18725326, 11937575, 15240678, 10352243, 17079865, 6218218, 19699150, 1672343, 3263424, 18467657, 10931159, 2208807, 21531893, 20451460, 20362509, 20424514, 9834067, 9743345, 1431145, 15979610, 20193009, 3931082, 9378961, 14734731, 17182544, 3872756, 6224882, 19926489, 1832281, 8125139, 17135226, 20440287, 3097874, 8120400, 3262942, 16023690, 15829271, 4883742, 11067962, 6167031, 3871450, 2303783, 2521387, 12408055, 6223073, 12594515, 15351311, 18566369, 2104919, 14597762, 12690201, 10430616, 18941180, 22576252, 16972052, 7650373, 17153857, 10924025
The chronic graft-versus-host disease (cGVHD) in mice is characterized by the production of autoantibodies and immunopathology characteristic of systemic lupus erythematosus (lupus). The basic pathogenesis involves the cognate recognition of foreign MHC class II of host B cells by alloreactive CD4 T cells from the donor. CD4 T cells of the host are also necessary for the full maturation of host B cells before the transfer of donor T cells. CD8 T cells play critical roles as well. Donor CD8 T cells that are highly cytotoxic can ablate or prevent the lupus syndrome, in part by killing recipient B cells. Host CD8 T cells can reciprocally downregulate donor CD8 T cells, and thus prevent them from suppressing the autoimmune process. Thus, when the donor inoculum contains both CD4 T cells and CD8 T cells, the resultant syndrome depends on the balance of activities of these various cell populations. For example, in one cGVHD model (DBA/2(C57BL/6xDBA/2)F1, the disease is more severe in females, as it is in several of the spontaneous mouse models of lupus, as well as in human disease. The mechanism of this female skewing of disease appears to depend on the relative inability of CD8 cells of the female host to downregulate the donor CD4 T cells that drive the autoantibody response. In general, then, the abnormal CD4 T cell help and the modulating roles of CD8 T cells seen in cGVHD parallel the participation of T cells in genetic lupus in mice and human lupus, although these spontaneous syndromes are presumably not driven by overt alloreactivity.
Animals, Autoantibodies, Autoimmunity, B-Lymphocytes, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Communication, Disease Models, Animal, Female, Graft vs Host Disease, Histocompatibility Antigens Class II, Lupus Erythematosus, Systemic, Mice, Sex Factors, Tissue Donors
null
22,704,963
2012-10-25
2014-11-20
1943-7811
The Journal of molecular diagnostics : JMD
Intratumoral heterogeneity of microRNA expression in breast cancer.
Raychaudhuri Mithu, Schuster Tibor, Buchner Theresa, Malinowsky Katharina, Bronger Holger, Schwarz-Boeger Ulrike, Höfler Heinz, Avril Stefanie
eng
null
Journal Article, Research Support, Non-U.S. Gov't
MicroRNAs
IM
22704963, S1525-1578(12)00098-0, 10.1016/j.jmoldx.2012.01.016
Profiling studies have identified specific microRNA (miRNA) signatures in malignant tumors including breast cancer. Our aim was to assess intratumoral heterogeneity in miRNA expression levels within primary breast cancers and between axillary lymph node metastases from the same patient. Specimens of 16 primary breast cancers were sampled in 8-10 distinct locations including the peripheral, intermediate, and central tumor zones, as well as two to five axillary lymph node metastases (n = 9). Total RNA was extracted from 132 paraffin-embedded samples, and the expression of miR-10b, miR-210, miR-31, and miR-335 was assessed as well as the reproducibility of RNA extraction and miRNA analysis by quantitative RT-PCR. Considerable intratumoral heterogeneity existed for all four miRNAs within primary breast cancers (CV 40%). No significant differences within (CV 34%) or between different tumor zones (CV 33%) were found. A similar variation in miRNA expression was observed between corresponding lymph node metastases (mean CV 40%). In comparison, the variation among different patients showed a CV of 80% for primary tumors and 103% for lymph node metastases. Both miRNA extraction procedures and quantitative RT-PCR showed high reproducibility (CV ≤ 2%). Thus, the intratumoral heterogeneity of miRNA expression in breast cancers can lead to significant sampling bias. Assessment of breast cancer miRNA profiles may require sampling at several different tumor locations and of several tumor-involved lymph nodes when deriving miRNA expression profiles of metastases.
Breast Neoplasms, Female, Humans, Immunohistochemistry, In Vitro Techniques, MicroRNAs, Reverse Transcriptase Polymerase Chain Reaction
null
22,704,964
2013-05-15
2012-11-29
1872-7646
Human movement science
Multiple representations and mechanisms for visuomotor adaptation in young children.
Tahej Pierre-Karim, Ferrel-Chapus Carole, Olivier Isabelle, Ginhac Dominique, Rolland Jean-Pierre
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704964, S0167-9457(12)00053-X, 10.1016/j.humov.2012.02.016
In this study, we utilized transformed spatial mappings to perturb visuomotor integration in 5-yr-old children and adults. The participants were asked to perform pointing movements under five different conditions of visuomotor rotation (from 0° to 180°), which were designed to reveal explicit vs. implicit representations as well as the mechanisms underlying the visual-motor mapping. Several tests allowed us to separately evaluate sensorimotor (i.e., the dynamic dimension of movement) and cognitive (i.e., the explicit representations of target position and the strategies used by the participants) representations of visuo-proprioceptive distortion. Our results indicate that children do not establish representations in the same manner as adults and that children exhibit multiple visuomotor representations. Sensorimotor representations were relatively precise, presumably due to the recovery of proprioceptive information and efferent copy. Furthermore, a bidirectional mechanism was used to re-map visual and motor spaces. In contrast, cognitive representations were supplied with visual information and followed a unidirectional visual-motor mapping. Therefore, it appears that sensorimotor mechanisms develop before the use of explicit strategies during development, and young children showed impaired visuomotor adaptation when confronted with large distortions.
Adaptation, Psychological, Child, Preschool, Depth Perception, Discrimination Learning, Female, Humans, Male, Orientation, Perceptual Distortion, Problem Solving, Proprioception, Psychomotor Performance, User-Computer Interface, Young Adult
null
22,704,965
2012-11-20
2021-12-03
1873-7544
Neuroscience
GDNF induces mechanical hyperalgesia in muscle by reducing I(BK) in isolectin B4-positive nociceptors.
Hendrich J, Alvarez P, Chen X, Levine J D
eng
P01 NS053709 (NINDS NIH HHS, United States); R01 AR054635 (NIAMS NIH HHS, United States); R01 AR063312 (NIAMS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural
Glial Cell Line-Derived Neurotrophic Factor, Glycoproteins, Lectins, Potassium Channels, Calcium-Activated, Vcan protein, rat, Versicans
IM
22704965, S0306-4522(12)00611-2, 10.1016/j.neuroscience.2012.06.011, PMC3404506, NIHMS393321, 20237269, 2445972, 12876402, 10562340, 14643767, 17303637, 18616564, 9870346, 15525272, 19820205, 12570985, 10893418, 11593232, 11021795, 20180019, 16157274, 20105244, 19679180, 22094329, 9526023, 17523149, 20800357, 8264961, 20861815, 1694175, 3306916, 16914685, 19524560, 9581756, 12482897, 11988777, 9782159, 2436105, 10414978, 18817728, 8137869, 22206923, 16635454, 21786301, 11703454, 19457113, 14561831, 12354627, 1381420, 20945047, 21604994, 12617957, 12904339, 18611207, 16362320, 14678770, 19818033, 16540576, 8114940
We have assessed the mechanism underlying glial cell-derived neurotrophic factor (GDNF)-induced mechanical hyperalgesia in the gastrocnemius muscle, using patch clamp electrophysiology, in vivo electrophysiology and behavioral studies. Cultured isolectin B4-positive (IB4+) dorsal root ganglion neurons that innervated this muscle were held under current clamp; the majority developed an increase in action potential duration (a factor of increase of 2.29±0.24, compared to 1.13±0.17 in control, P<0.01) in response to GDNF (200 ng/ml) by 15 min after application. They also demonstrated a depolarization of resting membrane potential, but without significant changes in rheobase, action potential peak, or after-hyperpolarization. Large-conductance voltage- and calcium-activated potassium (BK) channels, which have recently been shown to play a role in the repolarization of IB4+ nociceptors, were inhibited under voltage clamp, as indicated by a significant reduction in the iberiotoxin-sensitive current. In vivo single-fiber recording from muscle afferents revealed that injection of iberiotoxin into their peripheral nociceptive field caused an increase in nociceptor firing in response to a 60s suprathreshold stimulus (an increase from 392.2±119.8 spikes to 596.1±170.8 spikes, P<0.05). This was observed in the absence of changes in the mechanical threshold. Finally, injection of iberiotoxin into the gastrocnemius muscle produced dose-dependent mechanical hyperalgesia. These data support the suggestion that GDNF induces nociceptor sensitization and mechanical hyperalgesia, at least in part, by inhibiting BK current in IB4+ nociceptors.
Action Potentials, Animals, Ganglia, Spinal, Glial Cell Line-Derived Neurotrophic Factor, Glycoproteins, Hyperalgesia, Lectins, Male, Muscle, Skeletal, Nociceptors, Patch-Clamp Techniques, Potassium Channels, Calcium-Activated, Rats, Rats, Sprague-Dawley, Versicans
null
22,704,966
2012-12-14
2021-10-21
1873-7544
Neuroscience
Valproic acid increases white matter repair and neurogenesis after stroke.
Liu X S, Chopp M, Kassis H, Jia L F, Hozeska-Solgot A, Zhang R L, Chen C, Cui Y S, Zhang Z G
eng
P01 NS023393 (NINDS NIH HHS, United States); R01 AG037506 (NIA NIH HHS, United States); R01 NS075156 (NINDS NIH HHS, United States); P01 NS23393 (NINDS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Histone Deacetylase Inhibitors, Valproic Acid
IM
22704966, S0306-4522(12)00612-4, 10.1016/j.neuroscience.2012.06.012, PMC3412884, NIHMS386639, 16775151, 11447328, 8784142, 6539617, 14556938, 11460777, 12728267, 8370349, 16946032, 15897262, 9600215, 21521169, 17855562, 9070757, 17371805, 19384336, 12451133, 15215303, 11274346, 12580341, 15935060, 19549282, 12411660, 18174372, 19503085, 21853027, 17398106, 22171032, 16870736, 9878831, 15087713, 20552017, 12621301, 16367798, 15569354, 21051629, 20978517, 20360553, 17299453, 9040491, 9359590, 10884481, 16093378, 21411642, 17429000, 15189338, 12447935, 21423191, 21490970, 21423190
Acute treatment of stroke with histone deacetylase (HDAC) inhibitors has been shown to reduce ischemic cell damage; however, it is unclear whether delayed treatment with HDAC inhibitors will contribute to the brain repair and plasticity. In the present study, we investigated the effects of delayed treatment of stroke with a pan HDAC inhibitor, valproic acid (VPA), on white matter injury and neurogenesis during stroke recovery. Administration of VPA at a dose of 100mg/kg for 7 days starting 24h after middle cerebral artery occlusion (MCAo) in rats significantly improved neurological outcome measured 7-28 days post-MCAo. In addition, the VPA treatment significantly increased oligodendrocyte survival and newly generated oligodendrocytes, which was associated with elevation of myelinated axonal density in the ischemic boundary 28 days after MCAo. VPA treatment also increased the expression of glutamate transporter 1 (GLT1) in the ischemic boundary after stroke, and increased acetylated histone H4 expression in neuroblasts and the number of new neurons in striatal ischemic boundary region. This study provides new evidence that the delayed VPA treatment enhances white matter repair and neurogenesis in ischemic brain, which may contribute to improved functional outcome.
Animals, Brain, Histone Deacetylase Inhibitors, Immunohistochemistry, In Situ Nick-End Labeling, Male, Nerve Fibers, Myelinated, Neurogenesis, Rats, Recovery of Function, Stroke, Valproic Acid
null
22,704,967
2012-12-14
2024-11-05
1873-7544
Neuroscience
Increased inflammation and brain injury after transient focal cerebral ischemia in activating transcription factor 3 knockout mice.
Wang L, Deng S, Lu Y, Zhang Y, Yang L, Guan Y, Jiang H, Li H
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Activating Transcription Factor 3, Atf3 protein, mouse
IM
22704967, S0306-4522(12)00610-0, 10.1016/j.neuroscience.2012.06.010
Activating transcription factor 3 (ATF3) is a stress-induced transcription factor that has been shown to repress inflammatory gene expression in multiple cell types and diseases. This study was conducted to investigate the role of ATF3 in the pathological processes of cerebral ischemia and its influence on post-ischemic inflammation.
Activating Transcription Factor 3, Animals, Blotting, Western, Fluorescent Antibody Technique, In Situ Nick-End Labeling, Inflammation, Ischemic Attack, Transient, Mice, Mice, Knockout, Real-Time Polymerase Chain Reaction
null
22,704,968
2012-09-13
2012-07-09
1873-3468
FEBS letters
Synthetic biology - the state of play.
Kitney Richard, Freemont Paul
eng
null
Journal Article, Research Support, Non-U.S. Gov't, Review
null
IM
22704968, S0014-5793(12)00465-6, 10.1016/j.febslet.2012.06.002
Just over two years ago there was an article in Nature entitled "Five Hard Truths for Synthetic Biology". Since then, the field has moved on considerably. A number of economic commentators have shown that synthetic biology very significant industrial potential. This paper addresses key issues in relation to the state of play regarding synthetic biology. It first considers the current background to synthetic biology, whether it is a legitimate field and how it relates to foundational biological sciences. The fact that synthetic biology is a translational field is discussed and placed in the context of the industrial translation process. An important aspect of synthetic biology is platform technology, this topic is also discussed in some detail. Finally, examples of application areas are described.
Computer-Aided Design, Industry, Reference Standards, Synthetic Biology, Technology
null
22,704,970
2013-01-04
2018-12-01
1877-783X
Cancer epidemiology
The racial disparity in breast cancer mortality in the 25 largest cities in the United States.
Freund Karen M
eng
null
Letter, Comment
null
IM
22704970, S1877-7821(12)00068-9, 10.1016/j.canep.2012.05.007
null
Breast Neoplasms, Female, Health Status Disparities, Humans
null
22,704,969
2013-06-04
2012-12-24
1873-5800
The Knee
Intra-articular injections of hyaluronic acid induce positive clinical effects in knees of patients affected by haemophilic arthropathy.
Carulli Christian, Matassi Fabrizio, Civinini Roberto, Morfini Massimo, Tani Massimiliano, Innocenti Massimo
eng
null
Comparative Study, Journal Article
Viscosupplements, Hyaluronic Acid
IM
22704969, S0968-0160(12)00108-1, 10.1016/j.knee.2012.05.006
Haemophilic arthropathy is the most common clinical manifestation of haemophilia, secondary to recurrent haemarthrosis and chronic synovitis, and the knee represents the main target joint. Modern bleeding prevention has significatively limited the incidence of severe arthropathy, and primary approach is usually conservative. Viscosupplementation is felt as one of the most efficient treatments for the early stages of knee haemophilic arthropathy, based on short-term follow-up studies. The aim of this prospective case series study is to assess the clinical effectiveness of intra-articular administration of hyaluronic acid in the knee, evaluating long-term results, and focusing on the necessity of further treatments after viscosupplementation.
Adult, Aged, Female, Follow-Up Studies, Hemarthrosis, Hemophilia A, Humans, Hyaluronic Acid, Injections, Intra-Articular, Knee Joint, Male, Middle Aged, Prospective Studies, Range of Motion, Articular, Time Factors, Treatment Outcome, Viscosupplements, Young Adult
null
22,704,972
2012-12-14
2017-11-16
1879-1298
Chemosphere
Organ-wise accumulation of fluoride in Prosopis juliflora and its potential for phytoremediation of fluoride contaminated soil.
Saini Poonam, Khan Suphiya, Baunthiyal Mamta, Sharma Vinay
eng
null
Journal Article
Soil, Soil Pollutants, Fluorides
IM
22704972, S0045-6535(12)00655-8, 10.1016/j.chemosphere.2012.05.034
Fluoride (F) contamination is a global environmental problem, as there is no cure of fluorosis available yet. Prosopis juliflora is a leguminous perennial, phreatophyte tree, widely distributed in arid and semi-arid regions of world. It extensively grows in F endemic areas of Rajasthan (India) and has been known as a "green" solution to decontaminate cadmium, chromium and copper contaminated soils. This study aims to check the tolerance potential of P. juliflora to accumulate fluoride. For this work, P. juliflora seedlings were grown for 75 d on soilrite under five different concentrations of F viz., control, 25, 50, 75 and 100 mg NaF kg(-1). Organ-wise accumulation of F, bioaccumulation factor (BF), translocation factor (TF), growth ratio (GR) and F tolerance index (TI) were examined. Plant accumulated high amounts of F in roots. The organ-wise distribution showed an accumulation 4.41 mg kg(-1)dw, 12.97 mg kg(-1)dw and 16.75 mg kg(-1)dw F, in stem, leaves and roots respectively. The results indicated significant translocation of F from root into aerial parts. The bioaccumulation and translocation factor values (>1.0) showed high accumulation efficiency and tolerance of P. juliflora to F. It is concluded that P. juliflora is a suitable candidate for phytoremediation purpose and can be explored further for the decontamination of F polluted soils.
Biodegradation, Environmental, Biological Transport, Fluorides, Organ Specificity, Prosopis, Soil, Soil Pollutants
null
22,704,971
2013-01-04
2022-03-30
1877-783X
Cancer epidemiology
The role of hospital-based cancer registries in low and middle income countries-The Nigerian Case Study.
Jedy-Agba Elima E, Curado Maria-Paula, Oga Emmanuel, Samaila Modupeola O, Ezeome Emmanuel R, Obiorah Christopher, Erinomo Olagoke O, Ekanem Ima-Obong A, Uka Cornelius, Mayun Ahmed, Afolayan Enoch A, Abiodun Popoola, Olasode Babatunde J, Omonisi Abidemi, Otu Theresa, Osinubi Patience, Dakum Patrick, Blattner William, Adebamowo Clement A
eng
5D43TW001041-13 (FIC NIH HHS, United States); D43 CA153792 (NCI NIH HHS, United States); D43 TW001041 (FIC NIH HHS, United States); D43CA153792-01 (NCI NIH HHS, United States); U54 HG006947 (NHGRI NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural
null
IM
22704971, S1877-7821(12)00071-9, 10.1016/j.canep.2012.05.010, PMC3438360, NIHMS387668, 20231304, 21675400, 16916463, 19339719, 22098217, 21172092, 19256757, 21095532, 21351269, 20211030, 10735343, 21610859, 19535980, 10585584, 20482860, 20331326, 22075441, 21463833, 18672214, 11588851, 20485489, 21796634, 19123741, 22004990, 18797604, 1894320, 15827554, 11126081, 20229733
The incidence of cancer continues to rise all over the world and current projections show that there will be 1.27 million new cases and almost 1 million deaths by 2030. In view of the rising incidence of cancer in sub-Saharan Africa, urgent steps are needed to guide appropriate policy, health sector investment and resource allocation. We posit that hospital based cancer registries (HBCR) are fundamental sources of information on the frequent cancer sites in limited resource regions where population level data is often unavailable. In regions where population based cancer registries are not in existence, HBCR are beneficial for policy and planning.
Academic Medical Centers, Female, Humans, Incidence, Longitudinal Studies, Male, Neoplasms, Nigeria, Registries, Socioeconomic Factors
null
22,704,974
2013-02-26
2013-11-21
1879-1298
Chemosphere
Fate of citalopram during water treatment with O3, ClO2, UV and Fenton oxidation.
Hörsing Maritha, Kosjek Tina, Andersen Henrik R, Heath Ester, Ledin Anna
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Chlorine Compounds, Fenton's reagent, Oxides, Water Pollutants, Chemical, Water, Citalopram, Ozone, chlorine dioxide, Hydrogen Peroxide, Iron
IM
22704974, S0045-6535(12)00635-2, 10.1016/j.chemosphere.2012.05.024
In the present study we investigate the fate of citalopram (CIT) at neutral pH using advanced water treatment technologies that include O(3), ClO(2) oxidation, UV irradiation and Fenton oxidation. The ozonation resulted in 80% reduction after 30 min treatment. Oxidation with ClO(2) removed>90% CIT at a dosage of 0.1 mg L(-1). During UV irradiation 85% reduction was achieved after 5 min, while Fenton with addition of 14 mg L(-1) (Fe(2+)) resulted in 90% reduction of CIT. During these treatment experiments transformation products (TPs) were formed from CIT, where five compounds were identified by using high resolution and tandem mass spectrometry. Among these desmethyl-citalopram and citalopram N-oxide have been previously identified as human metabolites, while three are novel and published here for the first time. The three TPs are a hydroxylated dimethylamino-side chain derivative, a butyrolactone derivative and a defluorinated derivative of CIT.
Chlorine Compounds, Citalopram, Humans, Hydrogen Peroxide, Hydrogen-Ion Concentration, Iron, Oxidation-Reduction, Oxides, Ozone, Ultraviolet Rays, Waste Disposal, Fluid, Water, Water Pollutants, Chemical
null
22,704,973
2013-02-26
2013-11-21
1879-1298
Chemosphere
Ozonation of azo dye Acid Red 14 in a microporous tube-in-tube microchannel reactor: decolorization and mechanism.
Gao Meiping, Zeng Zequan, Sun Baochang, Zou Haikui, Chen Jianfeng, Shao Lei
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Acid red 14, Azo Compounds, Coloring Agents, Environmental Pollutants, Industrial Waste, Ozone
IM
22704973, S0045-6535(12)00719-9, 10.1016/j.chemosphere.2012.05.083
The ozonation of synthetic wastewater containing azo dye Acid Red 14 (AR 14) was investigated in a high-throughput microporous tube-in-tube microchannel reactor. The effects of design and operating parameters such as micropore size, annular channel width, liquid volumetric flow rate, ozone-containing gas volumetric flow rate, initial pH of the solution and initial AR 14 concentration on decolorization efficiency and ozone utilization efficiency were studied with the aim to optimize the operation conditions. An increase of the ozone-containing gas or liquid flow rate could greatly intensify the gas-liquid mass transfer. Reducing the micropore size and the annular channel width led to a higher mass transfer rate and was beneficial to decolorization. Decolorization efficiency increased with an increasing ozone-containing gas volumetric flow rate, as well as a decreasing liquid volumetric flow rate and initial AR 14 concentration. The optimum initial pH for AR 14 ozonation was determined as 9.0. The degradation kinetics was observed to be a pseudo-first-order reaction with respect to AR 14 concentration. The difference between the decolorization and COD removal efficiency indicated that many intermediates existed in AR 14 ozonation. The formation of six organic intermediates during ozonation was detected by GC/MS, while the concentration of nitrate and sulfate ions was determined by ion chromatography. The possible degradation mechanism of AR 14 in aqueous solution was proposed.
Azo Compounds, Color, Coloring Agents, Environmental Pollutants, Hydrogen-Ion Concentration, Industrial Waste, Kinetics, Microtechnology, Ozone, Porosity
null
22,704,975
2012-11-28
2019-12-10
1879-1298
Chemosphere
Assessing bioaccumulation of polybrominated diphenyl ethers for aquatic species by QSAR modeling.
Mansouri Kamel, Consonni Viviana, Durjava Mojca Kos, Kolar Boris, Öberg Tomas, Todeschini Roberto
eng
null
Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
Environmental Pollutants, Flame Retardants, Halogenated Diphenyl Ethers
IM
22704975, S0045-6535(12)00702-3, 10.1016/j.chemosphere.2012.05.081
Polybrominated diphenyl ethers (PBDEs) are used as flame retardants in textiles, foams and plastics. Highly bioaccumulative with toxic effects including developmental neurotoxicity estrogen and thyroid hormones disruption, they are considered as persistent organic pollutants (POPs) and have been found in human tissues, wildlife and biota worldwide. But only some of them are banned from EU market. For the environmental fate studies of these compounds the bioconcentration factor (BCF) is one of the most important endpoints to start with. We applied quantitative structure-activity relationships techniques to overcome the limited experimental data and avoid more animal testing. The aim of this work was to assess the bioaccumulation of PBDEs by means of QSAR. First, a BCF dataset of specifically conducted experiments was modeled. Then the study was extended by predicting the bioaccumulation and biomagnification factors using some experimental values from the literature. Molecular descriptors were calculated using DRAGON 6. The most relevant ones were selected and resulting models were compared paying attention to the applicability domain.
Animals, Aquatic Organisms, Environmental Exposure, Environmental Monitoring, Environmental Pollutants, Flame Retardants, Halogenated Diphenyl Ethers, Invertebrates, Models, Biological, Quantitative Structure-Activity Relationship, Vertebrates
null
22,704,976
2012-10-23
2016-11-25
1879-1298
Chemosphere
A pilot study for determining the optimal operation condition for simultaneously controlling the emissions of PCDD/Fs and PAHs from the iron ore sintering process.
Chen Yu-Cheng, Tsai Perng-Jy, Mou Jin-Luh, Kuo Yu-Chieh, Wang Shih-Min, Young Li-Hao, Wang Ya-Fen
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Air Pollutants, Industrial Waste, Polychlorinated Dibenzodioxins, Polycyclic Aromatic Hydrocarbons, Iron
IM
22704976, S0045-6535(12)00652-2, 10.1016/j.chemosphere.2012.05.031
In this study, the cost-benefit analysis technique was developed and incorporated into the Taguchi experimental design to determine the optimal operation combination for the purpose of providing a technique solution for controlling both emissions of PCDD/Fs and PAHs, and increasing both the sinter productivity (SP) and sinter strength (SS) simultaneously. Four operating parameters, including the water content, suction pressure, bed height, and type of hearth layer, were selected and all experimental campaigns were conducted on a pilot-scale sinter pot to simulate various sintering operating conditions of a real-scale sinter plant. The resultant optimal combination could reduce the total carcinogenic emissions arising from both emissions of PCDD/Fs and PAHs by 49.8%, and increase the sinter benefit associated with the increase in both SP and SS by 10.1%, as in comparison with the operation condition currently used in the real plant. The ANOVA results indicate that the suction pressure was the most dominant parameter in determining the optimal operation combination. The above result was theoretically plausible since the higher suction pressure provided more oxygen contents leading to the decrease in both PCDD/F and PAH emissions. But it should be noted that the results obtained from the present study were based on pilot scale experiments, conducting confirmation tests in a real scale plant are still necessary in the future.
Air Pollutants, Cost-Benefit Analysis, Environmental Monitoring, Industrial Waste, Iron, Polychlorinated Dibenzodioxins, Polycyclic Aromatic Hydrocarbons
null
22,704,977
2012-11-20
2019-12-10
1873-5177
Pharmacology, biochemistry, and behavior
The investigation of neonatal MK-801 administration and physical environmental enrichment on emotional and cognitive functions in adult Balb/c mice.
Akillioglu Kubra, Babar Melik Emine, Melik Enver, Kocahan Sayad
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Neuroprotective Agents, Dizocilpine Maleate
IM
22704977, S0091-3057(12)00154-2, 10.1016/j.pbb.2012.06.006
N-methyl-D-aspartate (NMDA) receptors play an important role in brain maturation and developmental processes. It is known that growing up in an enriched environment has effects on emotional and cognitive performance. In our study, we evaluated the effects of physically enriched environment on the emotional and cognitive functions of the adult brain in the setting of previous NMDA receptor hypoactivity during the critical developmental period of the nervous system. In this study, NMDA receptor blockade was induced 5-10 days postnatally (PD5-10) using MK-801 in mice Balb/c (twice a day 0.25 mg/kg, for 5 days, intraperitoneal). MK-801 was given to developing mice living in a standard (SE) and an enrichment environment (EE) and once the animals reached adulthood, emotional behaviors were evaluated using an open field test (OF) and an elevated plus maze (EPM) test whereas cognitive processes were evaluated using the Morris water-maze (MWM). The EE group showed decreased locomotor activity (p<0.05) in the OF and increased exploratory behaviour (p<0.01) and decreased fear of heights/anxiety-like behaviour (p<0.05) in the EPM test. The EE had positive effects on spatial learning in the MWM (p<0.05). Blockade of the NMDA receptor increased the fear of height (p<0.05), decreased exploratory behaviour and locomotor activity (p<0.001). Also, it led to decreased spatial learning (p<0.05). The decreases in spatial learning and exploratory behaviours and the increase in fear of heights/anxiety-like behaviour with NMDA receptor blockade was not reversed by EE. NMDA receptor blockade during the critical period of development led to deterioration in the emotional and cognitive processes during adulthood. An enriched environmental did not reverse the deleterious effects of the NMDA receptor blockade on emotional and cognitive functions.
Animals, Animals, Newborn, Anxiety, Body Weight, Brain, Cognition, Critical Period, Psychological, Dizocilpine Maleate, Emotions, Environment, Exploratory Behavior, Growth, Learning, Male, Maze Learning, Memory, Mice, Mice, Inbred BALB C, Motor Activity, Neuroprotective Agents, Swimming
null
22,704,978
2013-01-29
2021-10-21
1096-7206
Molecular genetics and metabolism
Intravenous high-dose enzyme replacement therapy with recombinant palmitoyl-protein thioesterase reduces visceral lysosomal storage and modestly prolongs survival in a preclinical mouse model of infantile neuronal ceroid lipofuscinosis.
Hu Jie, Lu Jui-Yun, Wong Andrew M S, Hynan Linda S, Birnbaum Shari G, Yilmaz Denis S, Streit Barbara M, Lenartowicz Ewelina M, Thompson Thomas C M, Cooper Jonathan D, Hofmann Sandra L
eng
R01 NS036867 (NINDS NIH HHS, United States); R37 NS036867 (NINDS NIH HHS, United States); NS036867 (NINDS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Recombinant Proteins, Thiolester Hydrolases, palmitoyl-protein thioesterase
IM
22704978, S1096-7192(12)00198-9, 10.1016/j.ymgme.2012.05.009, PMC3444630, NIHMS379947, 7757942, 18341399, 20494602, 22310926, 22368049, 22331300, 8816748, 21946346, 4763201, 4121459, 19640925, 15965709, 15649713, 21749451, 21482164, 9870693, 9664077, 17261688, 7315333, 11589001, 16881055, 18443601, 11506414, 21990111, 15193292, 18341400, 2166050, 18362923, 11717424, 17990914, 20036592, 18402812, 16364693, 17046272, 21123810, 10191108, 19416667, 15193291, 16627894, 21575633, 7637805, 15314220, 21784683, 6319015
PPT1-related neuronal ceroid lipofuscinosis (NCL) is a lysosomal storage disorder caused by deficiency in a soluble lysosomal enzyme, palmitoyl-protein thioesterase-1 (PPT1). Enzyme replacement therapy (ERT) has not been previously examined in a preclinical animal model. Homozygous PPT1 knockout mice reproduce the known features of the disease, developing signs of motor dysfunction at 5 months of age and death by around 8 months. In the current study, PPT1 knockout mice were treated with purified recombinant PPT1 (0.3 mg, corresponding to 12 mg/kg or 180 U/kg for a 25 g mouse) administered intravenously weekly either 1) from birth; or 2) beginning at 8 weeks of age. The treatment was surprisingly well tolerated and neither anaphylaxis nor antibody formation was observed. In mice treated from birth, survival increased from 236 to 271 days (p<0.001) and the onset of motor deterioration was similarly delayed. In mice treated beginning at 8 weeks, no increases in survival or motor performance were seen. An improvement in neuropathology in the thalamus was seen at 3 months in mice treated from birth, and although this improvement persisted it was attenuated by 7 months. Outside the central nervous system, substantial clearance of autofluorescent storage material in many tissues was observed. Macrophages in spleen, liver and intestine were especially markedly improved, as were acinar cells of the pancreas and tubular cells of the kidney. These findings suggest that ERT may be an option for addressing visceral storage as part of a comprehensive approach to PPT1-related NCL, but more effective delivery methods to target the brain are needed.
Animals, Brain, Disease Models, Animal, Enzyme Replacement Therapy, Female, Humans, Lysosomes, Male, Mice, Mice, Knockout, Motor Activity, Neuronal Ceroid-Lipofuscinoses, Recombinant Proteins, Rotarod Performance Test, Thiolester Hydrolases, Viscera
null
22,704,979
2012-12-26
2021-10-21
1567-7257
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
The emergence and maintenance of sickle cell hotspots in the Mediterranean.
Penman Bridget S, Gupta Sunetra, Buckee Caroline O
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704979, S1567-1348(12)00216-X, 10.1016/j.meegid.2012.06.001, PMC3438445, 22133230, 9623998, 17504739, 2473806, 5296398, 6134037, 14655880, 15942909, 6584911, 10872472, 12149194, 21427751, 3713863, 21872606, 3957693, 11545326, 5780379, 789227, 18353707, 17519411, 5808784, 18376107, 1687685, 22445352, 21907014, 19955437, 16227994, 14102772, 15247422, 13115700, 11713529, 13969412, 16021928, 2898955, 2680886, 5443168, 22188117, 6297530
Genetic disorders of haemoglobin (haemoglobinopathies), including the thalassaemias and sickle cell anaemia, abound in historically malarious regions, due to the protection they provide against death from severe malaria. Despite the overall spatial correlation between malaria and these disorders, inter-population differences exist in the precise combinations of haemoglobinopathies observed. Greece and Italy present a particularly interesting case study: their high frequencies of beta thalassaemia speak to a history of intense malaria selection, yet they possess very little of the strongly malaria protective mutation responsible for sickle cell anaemia, despite historical migrational links with Africa where high frequencies of sickle cell occur. Twentieth century surveys of beta thalassaemia and sickle cell in Greece, Sicily and Sardinia have revealed striking sickle cell 'hotspots' - places where the frequency of sickle cell approaches that seen in Africa while neighbouring populations remain relatively sickle cell free. It remains unclear how these hotspots have been maintained over time without sickle cell spreading throughout the region. Here we use a metapopulation model to show that (i) epistasis between the alpha and beta forms of thalassaemia can restrict the spread of sickle cell through a network of linked subpopulations and (ii) the emergence of sickle cell hotspots requires relatively low levels of gene flow, but the aforementioned epistasis increases the chances of hotspots forming.
Computer Simulation, Endemic Diseases, Epistasis, Genetic, Gene Flow, Gene Frequency, Greece, Italy, Mediterranean Islands, Mediterranean Region, Models, Genetic, alpha-Thalassemia, beta-Thalassemia
null
22,704,981
2012-12-20
2022-03-18
1879-0402
Current opinion in chemical biology
The XNA world: progress towards replication and evolution of synthetic genetic polymers.
Pinheiro Vitor B, Holliger Philipp
eng
MC_U105178804 (Medical Research Council, United Kingdom); U105178804 (Medical Research Council, United Kingdom)
Journal Article, Research Support, Non-U.S. Gov't, Review
Polymers, RNA, DNA
IM
22704981, S1367-5931(12)00072-5, 10.1016/j.cbpa.2012.05.198
Life's diversity is built on the wide range of properties and functions that can be encoded in natural biopolymers such as polypeptides and nucleic acids. However, despite their versatility, the range of chemical functionalities is limited, particularly in the case of nucleic acids. Chemical modification of nucleic acids can greatly increase their functional diversity but access to the full phenotypic potential of such polymers requires a system of replication. Here we review progress in the chemical and enzymatic synthesis, replication and evolution of unnatural nucleic acid polymers, which promises to enable the exploration of a vast sequence space not accessible to nature and deliver ligands, catalysts and materials based on this new class of biopolymers.
DNA, DNA Replication, Evolution, Molecular, Polymers, RNA
null
22,704,980
2013-12-02
2013-01-14
1578-1968
Neurologia (Barcelona, Spain)
La Salpêtrière Hospital before Charcot: a visit described by Pedro González Velasco.
Giménez-Roldán S
eng
null
Biography, Historical Article, Journal Article
null
IM
22704980, S0213-4853(12)00117-X, 10.1016/j.nrl.2012.03.017
Under Charcot's leadership, La Salpêtrière was transformed into one of the world's top neurology centres. However, there is little information regarding the patient care facilities which Charcot would have encountered upon his arrival in 1862.
France, History, 19th Century, Hospitals, Psychiatric, Humans, Mental Disorders, Patient Care, Spain
null
22,704,982
2012-10-26
2024-01-09
1879-355X
International journal of radiation oncology, biology, physics
International patterns of practice in the management of radiation therapy-induced nausea and vomiting.
Dennis Kristopher, Zhang Liying, Lutz Stephen, van Baardwijk Angela, van der Linden Yvette, Holt Tanya, Arnalot Palmira Foro, Lagrange Jean-Léon, Maranzano Ernesto, Liu Rico, Wong Kam-Hung, Wong Lea-Choung, Vassiliou Vassilios, Corn Benjamin W, De Angelis Carlo, Holden Lori, Wong C Shun, Chow Edward
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Antiemetics, Serotonin 5-HT3 Receptor Antagonists
IM
22704982, S0360-3016(12)00253-2, 10.1016/j.ijrobp.2012.02.031
To investigate international patterns of practice in the management of radiation therapy-induced nausea and vomiting (RINV).
Adult, Aged, Aged, 80 and over, Antiemetics, Disease Management, Female, Guideline Adherence, Health Care Surveys, Humans, Internationality, Male, Middle Aged, Nausea, Practice Guidelines as Topic, Practice Patterns, Physicians', Radiotherapy, Salvage Therapy, Serotonin 5-HT3 Receptor Antagonists, Vomiting
null
22,704,983
2013-03-17
2022-03-31
1879-355X
International journal of radiation oncology, biology, physics
Symptomatic outcomes in relation to tumor expansion after fractionated stereotactic radiation therapy for vestibular schwannomas: single-institutional long-term experience.
Aoyama Hidefumi, Onodera Shunsuke, Takeichi Norihito, Onimaru Rikiya, Terasaka Shunsuke, Sawamura Yutaka, Shirato Hiroki
eng
null
Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704983, S0360-3016(12)00645-1, 10.1016/j.ijrobp.2012.05.003
The effect of transient tumor expansion after conventionally fractionated stereotactic radiation therapy (SRT) on the symptomatic outcomes is not well-known.
Adolescent, Adult, Aged, Aged, 80 and over, Disease Progression, Dizziness, Dose Fractionation, Radiation, Facial Nerve Diseases, Female, Hearing, Hearing Loss, Humans, Hydrocephalus, Male, Middle Aged, Multivariate Analysis, Neoplasm Regression, Spontaneous, Neuroma, Acoustic, Radiosurgery, Salvage Therapy, Tinnitus, Trigeminal Nerve, Trigeminal Nerve Diseases, Tumor Burden, Young Adult
null
22,704,984
2013-03-17
2018-12-02
1879-355X
International journal of radiation oncology, biology, physics
A multi-institutional study of factors influencing the use of stereotactic radiosurgery for brain metastases.
Hodgson David C, Charpentier Anne-Marie, Cigsar Candemir, Atenafu Eshetu G, Ng Angela, Bahl Guarav, Zadeh Gelareh, San Miguel John, Menard Cynthia
eng
null
Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't
null
IM
22704984, S0360-3016(12)00644-X, 10.1016/j.ijrobp.2012.05.002
Stereotactic radiosurgery (SRS) for brain metastases is a relatively well-studied technology with established guidelines regarding patient selection, although its implementation is technically complex. We evaluated the extent to which local availability of SRS affected the treatment of patients with brain metastases.
Adult, Age Factors, Aged, Aged, 80 and over, Analysis of Variance, Brain Neoplasms, Breast Neoplasms, Carcinoma, Non-Small-Cell Lung, Carcinoma, Renal Cell, Colorectal Neoplasms, Cranial Irradiation, Female, Humans, Kidney Neoplasms, Lung Neoplasms, Male, Melanoma, Middle Aged, Odds Ratio, Ontario, Radiosurgery, Regression Analysis
null
22,704,985
2013-02-12
2021-10-21
1879-0445
Current biology : CB
A novel role for Bcl-2 in regulation of cellular calcium extrusion.
Ferdek Pawel E, Gerasimenko Julia V, Peng Shuang, Tepikin Alexei V, Petersen Ole H, Gerasimenko Oleg V
eng
G19/22 (Medical Research Council, United Kingdom); G0700167 (Medical Research Council, United Kingdom); G0300076 (Medical Research Council, United Kingdom); MR/J002771/1 (Medical Research Council, United Kingdom); G8801575 (Medical Research Council, United Kingdom)
Journal Article, Research Support, Non-U.S. Gov't
Inositol 1,4,5-Trisphosphate Receptors, Peptides, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, caloxin 3A1, Bcl2 protein, mouse, Barium, Meglumine, Vitamin K 3, Ca(2+) Mg(2+)-ATPase, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Calcium
IM
22704985, S0960-9822(12)00523-4, 10.1016/j.cub.2012.05.002, PMC3396842, 19789383, 18955969, 21964458, 12765680, 21763611, 11917155, 17924581, 9056075, 8894275, 15263017, 19765991, 10823933, 9298978, 15585581, 11248055, 15536077, 9788433, 19515844, 21818117, 20617337, 18657507, 21415859, 16388593, 11861756, 15670871, 10704437, 17431216, 17343911
The antiapoptotic protein Bcl-2 plays important roles in Ca(2+) signaling by influencing inositol triphosphate receptors and regulating Ca(2+)-induced Ca(2+) release. Here we investigated whether Bcl-2 affects Ca(2+) extrusion in pancreatic acinar cells. We specifically blocked the Ca(2+) pumps in the endoplasmic reticulum and assessed the rate at which the cells reduced an elevated cytosolic Ca(2+) concentration after a period of enhanced Ca(2+) entry. Because external Ca(2+) was removed and endoplasmic reticulum Ca(2+) pumps were blocked, Ca(2+) extrusion was the only process responsible for recovery. Cells lacking Bcl-2 restored the basal cytosolic Ca(2+) level much faster than control cells. The enhanced Ca(2+) extrusion in cells from Bcl-2 knockout (Bcl-2 KO) mice was not due to increased Na(+)/Ca(2+) exchange activity, because removal of external Na(+) did not influence the Ca(2+) extrusion rate. Overexpression of Bcl-2 in the pancreatic acinar cell line AR42J decreased Ca(2+) extrusion, whereas silencing Bcl-2 expression (siRNA) had the opposite effect. Loss of Bcl-2, while increasing Ca(2+) extrusion, dramatically decreased necrosis and promoted apoptosis induced by oxidative stress, whereas specific inhibition of Ca(2+) pumps in the plasma membrane (PMCA) with caloxin 3A1 reduced Ca(2+) extrusion and increased necrosis. Bcl-2 regulates PMCA function in pancreatic acinar cells and thereby influences cell fate.
Acinar Cells, Animals, Apoptosis, Barium, Ca(2+) Mg(2+)-ATPase, Calcium, Calcium Signaling, Cell Membrane, Cytosol, Endoplasmic Reticulum, Inositol 1,4,5-Trisphosphate Receptors, Meglumine, Mice, Mice, Inbred C57BL, Mice, Knockout, Necrosis, Pancreas, Peptides, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Vitamin K 3
null
22,704,986
2012-11-30
2017-11-16
1879-0445
Current biology : CB
Genomic and morphological evidence converge to resolve the enigma of Strepsiptera.
Niehuis Oliver, Hartig Gerrit, Grath Sonja, Pohl Hans, Lehmann Jörg, Tafer Hakim, Donath Alexander, Krauss Veiko, Eisenhardt Carina, Hertel Jana, Petersen Malte, Mayer Christoph, Meusemann Karen, Peters Ralph S, Stadler Peter F, Beutel Rolf G, Bornberg-Bauer Erich, McKenna Duane D, Misof Bernhard
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704986, S0960-9822(12)00571-4, 10.1016/j.cub.2012.05.018
The phylogeny of insects, one of the most spectacular radiations of life on earth, has received considerable attention. However, the evolutionary roots of one intriguing group of insects, the twisted-wing parasites (Strepsiptera), remain unclear despite centuries of study and debate. Strepsiptera exhibit exceptional larval developmental features, consistent with a predicted step from direct (hemimetabolous) larval development to complete metamorphosis that could have set the stage for the spectacular radiation of metamorphic (holometabolous) insects. Here we report the sequencing of a Strepsiptera genome and show that the analysis of sequence-based genomic data (comprising more than 18 million nucleotides from nearly 4,500 genes obtained from a total of 13 insect genomes), along with genomic metacharacters, clarifies the phylogenetic origin of Strepsiptera and sheds light on the evolution of holometabolous insect development. Our results provide overwhelming support for Strepsiptera as the closest living relatives of beetles (Coleoptera). They demonstrate that the larval developmental features of Strepsiptera, reminiscent of those of hemimetabolous insects, are the result of convergence. Our analyses solve the long-standing enigma of the evolutionary roots of Strepsiptera and reveal that the holometabolous mode of insect development is more malleable than previously thought.
Animals, Biological Evolution, Genome, Insect, Genome, Mitochondrial, Insecta, Molecular Sequence Data, Phylogeny, Sequence Alignment, Sequence Analysis, DNA, Sequence Analysis, Protein
null
22,704,987
2012-11-30
2024-06-10
1879-0445
Current biology : CB
The tangential nucleus controls a gravito-inertial vestibulo-ocular reflex.
Bianco Isaac H, Ma Leung-Hang, Schoppik David, Robson Drew N, Orger Michael B, Beck James C, Li Jennifer M, Schier Alexander F, Engert Florian, Baker Robert
eng
1R01DA030304-01 (NIDA NIH HHS, United States); R01 EY002007 (NEI NIH HHS, United States); R01 DA030304 (NIDA NIH HHS, United States); RC2NS069407 (NINDS NIH HHS, United States); 1RC2NS069407-01 (NINDS NIH HHS, United States); RC2 NS069407 (NINDS NIH HHS, United States); R01 HL109525 (NHLBI NIH HHS, United States); R01 GM085357 (NIGMS NIH HHS, United States); EY002007 (NEI NIH HHS, United States); R01GM08535 (NIGMS NIH HHS, United States); HL109525 (NHLBI NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
null
IM
22704987, S0960-9822(12)00579-9, 10.1016/j.cub.2012.05.026, PMC3647252, NIHMS462934, 3217526, 18264094, 1491254, 20975699, 8589427, 9914299, 14784863, 10433906, 10627598, 8821445, 15212435, 10022570, 21124455, 1619042, 16221589, 8694425, 15602884, 15558734, 21857656, 824412, 11718771, 4044944, 10934000, 19705454, 9828048, 19378103, 17981678, 9870961, 20970321, 12106078, 10409097, 17601957, 15269231, 18632885, 18685033, 18338968, 12930818, 11291722, 11477595, 1344070, 20815905, 10861559, 15282606, 12853438, 904774, 6784658, 20484654
Although adult vertebrates sense changes in head position by using two classes of accelerometer, at larval stages zebrafish lack functional semicircular canals and rely exclusively on their otolithic organs to transduce vestibular information.
Animals, Eye Movements, Gravitation, Larva, Laser Therapy, Otolithic Membrane, Reflex, Vestibulo-Ocular, Saccule and Utricle, Signal Transduction, Vestibular Nuclei, Visual Perception, Zebrafish
null
22,704,988
2013-02-12
2021-12-03
1879-0445
Current biology : CB
AtABCG29 is a monolignol transporter involved in lignin biosynthesis.
Alejandro Santiago, Lee Yuree, Tohge Takayuki, Sudre Damien, Osorio Sonia, Park Jiyoung, Bovet Lucien, Lee Youngsook, Geldner Niko, Fernie Alisdair R, Martinoia Enrico
eng
null
Journal Article, Research Support, Non-U.S. Gov't
ATP-Binding Cassette Transporters, Arabidopsis Proteins, Coumaric Acids, Propionates, Lignin, p-coumaric acid
IM
22704988, S0960-9822(12)00520-9, 10.1016/j.cub.2012.04.064
Lignin is the defining constituent of wood and the second most abundant natural polymer on earth. Lignin is produced by the oxidative coupling of three monolignols: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Monolignols are synthesized via the phenylpropanoid pathway and eventually polymerized in the cell wall by peroxidases and laccases. However, the mechanism whereby monolignols are transported from the cytosol to the cell wall has remained elusive. Here we report the discovery that AtABCG29, an ATP-binding cassette transporter, acts as a p-coumaryl alcohol transporter. Expression of AtABCG29 promoter-driven reporter genes and a Citrine-AtABCG29 fusion construct revealed that AtABCG29 is targeted to the plasma membrane of the root endodermis and vascular tissue. Moreover, yeasts expressing AtABCG29 exhibited an increased tolerance to p-coumaryl alcohol by excreting this monolignol. Vesicles isolated from yeasts expressing AtABCG29 exhibited a p-coumaryl alcohol transport activity. Loss-of-function Arabidopsis mutants contained less lignin subunits and were more sensitive to p-coumaryl alcohol. Changes in secondary metabolite profiles in abcg29 underline the importance of regulating p-coumaryl alcohol levels in the cytosol. This is the first identification of a monolignol transporter, closing a crucial gap in our understanding of lignin biosynthesis, which could open new directions for lignin engineering.
ATP-Binding Cassette Transporters, Arabidopsis, Arabidopsis Proteins, Biological Transport, Coumaric Acids, Gene Expression Regulation, Plant, Lignin, Mutation, Promoter Regions, Genetic, Propionates, Protein Transport, Yeasts
null
22,704,989
2012-12-26
2021-10-21
1879-0445
Current biology : CB
Tropomyosin is essential for processive movement of a class V myosin from budding yeast.
Hodges Alex R, Krementsova Elena B, Bookwalter Carol S, Fagnant Patricia M, Sladewski Thomas E, Trybus Kathleen M
eng
R01 GM078097 (NIGMS NIH HHS, United States); GM078097 (NIGMS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural
Actins, MYO2 protein, S cerevisiae, Protein Isoforms, Saccharomyces cerevisiae Proteins, TPM1 protein, S cerevisiae, Tropomyosin, Myosin Type V, Myosin Heavy Chains
IM
22704989, S0960-9822(12)00588-X, 10.1016/j.cub.2012.05.035, PMC3570268, NIHMS380859, 6452159, 19478066, 10570140, 11509238, 11781333, 16378722, 20705471, 14535950, 15473852, 12791999, 15980429, 19209828, 20005107, 17640878, 11740494, 21757693, 18201966, 22084309, 6118372, 16190472, 10448864, 11381095, 7615669, 20858426, 15953552, 18599451, 17178912, 17293871, 11457840, 18079121, 15764654, 18239852, 9864365, 7844152, 12660775, 20364131, 7820856, 16799566, 10986121
Myosin V is an actin-based motor protein involved in intracellular cargo transport [1]. Given this physiological role, it was widely assumed that all class V myosins are processive, able to take multiple steps along actin filaments without dissociating. This notion was challenged when several class V myosins were characterized as nonprocessive in vitro, including Myo2p, the essential class V myosin from S. cerevisiae [2-6]. Myo2p moves cargo including secretory vesicles and other organelles for several microns along actin cables in vivo. This demonstrated cargo transporter must therefore either operate in small ensembles or behave processively in the cellular context. Here we show that Myo2p moves processively in vitro as a single motor when it walks on an actin track that more closely resembles the actin cables found in vivo. The key to processivity is tropomyosin: Myo2p is not processive on bare actin but highly processive on actin-tropomyosin. The major yeast tropomyosin isoform, Tpm1p, supports the most robust processivity. Tropomyosin slows the rate of MgADP release, thus increasing the time the motor spends strongly attached to actin. This is the first example of tropomyosin switching a motor from nonprocessive to processive motion on actin.
Actins, Myosin Heavy Chains, Myosin Type V, Protein Isoforms, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Tropomyosin
null
22,704,991
2012-12-26
2021-10-21
1879-0445
Current biology : CB
Endocytosis of G protein-coupled receptors is regulated by clathrin light chain phosphorylation.
Ferreira Filipe, Foley Matthew, Cooke Alex, Cunningham Margaret, Smith Gemma, Woolley Robert, Henderson Graeme, Kelly Eamonn, Mundell Stuart, Smythe Elizabeth
eng
G0300452 (Medical Research Council, United Kingdom); G0600943 (Medical Research Council, United Kingdom); FS/11/49/28751 (British Heart Foundation, United Kingdom); PG/09/091/28074 (British Heart Foundation, United Kingdom); GR077544AIA (Wellcome Trust, United Kingdom)
Journal Article, Research Support, Non-U.S. Gov't
Clathrin Light Chains, Receptors, G-Protein-Coupled, G-Protein-Coupled Receptor Kinase 2
IM
22704991, S0960-9822(12)00587-8, 10.1016/j.cub.2012.05.034
Signaling by transmembrane receptors such as G protein-coupled receptors (GPCRs) occurs at the cell surface and throughout the endocytic pathway, and signaling from the cell surface may differ in magnitude and downstream output from intracellular signaling. As a result, the rate at which signaling molecules traverse the endocytic pathway makes a significant contribution to downstream output. Modulation of the core endocytic machinery facilitates differential uptake of individual cargoes. Clathrin-coated pits are a major entry portal where assembled clathrin forms a lattice around invaginating buds that have captured endocytic cargo. Clathrin assembles into triskelia composed of three clathrin heavy chains and associated clathrin light chains (CLCs). Despite the identification of clathrin-coated pits at the cell surface over 30 years ago, the functions of CLCs in endocytosis have been elusive.
Animals, Clathrin Light Chains, Clathrin-Coated Vesicles, Endocytosis, G-Protein-Coupled Receptor Kinase 2, Phosphorylation, Receptors, G-Protein-Coupled
null
22,704,990
2012-12-26
2022-01-29
1879-0445
Current biology : CB
Stochastic, adaptive sampling of information by microvilli in fly photoreceptors.
Song Zhuoyi, Postma Marten, Billings Stephen A, Coca Daniel, Hardie Roger C, Juusola Mikko
eng
BB/H013849/1 (Biotechnology and Biological Sciences Research Council, United Kingdom); E19850 (Biotechnology and Biological Sciences Research Council, United Kingdom); BB/F012071/1 (Biotechnology and Biological Sciences Research Council, United Kingdom); BB/D001900/1 (Biotechnology and Biological Sciences Research Council, United Kingdom); BB/G006865/1 (Biotechnology and Biological Sciences Research Council, United Kingdom); BB/D007585/1 (Biotechnology and Biological Sciences Research Council, United Kingdom)
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704990, S0960-9822(12)00634-3, 10.1016/j.cub.2012.05.047, PMC3420010, 9425553, 2892201, 11086990, 20648395, 7350542, 19180196, 7807062, 19180195, 18653755, 1486129, 11134229, 7441194, 2362184, 21957252, 8218908, 11931743, 21368135, 18478123, 1902848, 12860926, 11369048, 16249881, 16525044, 12571596, 7108487, 17923089, 21703451, 16636201, 418142, 12554647, 17522302, 16005297, 1314617, 11134228, 10747191, 20144772
In fly photoreceptors, light is focused onto a photosensitive waveguide, the rhabdomere, consisting of tens of thousands of microvilli. Each microvillus is capable of generating elementary responses, quantum bumps, in response to single photons using a stochastically operating phototransduction cascade. Whereas much is known about the cascade reactions, less is known about how the concerted action of the microvilli population encodes light changes into neural information and how the ultrastructure and biochemical machinery of photoreceptors of flies and other insects evolved in relation to the information sampling and processing they perform.
Adaptation, Physiological, Animals, Drosophila, Feedback, Physiological, Microvilli, Models, Biological, Photoreceptor Cells, Invertebrate, Stochastic Processes
null
22,704,992
2013-02-12
2017-11-16
1879-0445
Current biology : CB
Intraspecific directed deterrence by the mustard oil bomb in a desert plant.
Samuni-Blank Michal, Izhaki Ido, Dearing M Denise, Gerchman Yoram, Trabelcy Beny, Lotan Alon, Karasov William H, Arad Zeev
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Plant Oils, mustard oil
IM
22704992, S0960-9822(12)00471-X, 10.1016/j.cub.2012.04.051
Plant secondary metabolites (SMs) acting as defensive chemicals in reproductive organs such as fruit tissues play roles in both mutualistic and antagonistic interactions between plants and seed dispersers/predators. The directed-deterrence hypothesis states that SMs in ripe fruits deter seed predators but have little or no effect on seed dispersers. Indeed, studies have demonstrated that birds are able to cope with fruit SMs whereas rodents are deterred by them. However, this mechanism was only demonstrated at the class level, i.e., between birds and mammals, based on differences in the vanilloid receptors. Here we present experimental and behavioral data demonstrating the use of the broad-range, class-independent "mustard oil bomb" mechanism in Ochradenus baccatus fruits to force a behavioral change at an ecological timescale, converting rodents from seed predators to seed dispersers. This is achieved by a unique compartmentalization of the mustard oil bomb, causing activation of the system only upon seed and pulp coconsumption, encouraging seed dispersal via seed spitting by rodents. Our findings demonstrate the power of SMs to shift the animal-plant relationship from predation to mutualism and provide support for the directed-deterrence hypothesis at the intraspecific level, in addition to the interspecific level.
Animals, Desert Climate, Fruit, Magnoliopsida, Mustard Plant, Plant Oils, Rodentia, Seed Dispersal, Seeds
null
22,704,993
2012-11-30
2012-07-27
1879-0445
Current biology : CB
A specialized area in limbic cortex for fast analysis of peripheral vision.
Yu Hsin-Hao, Chaplin Tristan A, Davies Amanda J, Verma Richa, Rosa Marcello G P
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22704993, S0960-9822(12)00582-9, 10.1016/j.cub.2012.05.029
In primates, prostriata is a small area located between the primary visual cortex (V1) and the hippocampal formation. Prostriata sends connections to multisensory and high-order association areas in the temporal, parietal, cingulate, orbitofrontal, and frontopolar cortices. It is characterized by a relatively simple histological organization, alluding to an early origin in mammalian evolution. Here we show that prostriata neurons in marmoset monkeys exhibit a unique combination of response properties, suggesting a new pathway for rapid distribution of visual information in parallel with the traditionally recognized dorsal and ventral streams. Whereas the location and known connections of prostriata suggest a high-level association area, its response properties are unexpectedly simple, resembling those found in early stages of the visual processing: neurons have robust, nonadapting responses to simple stimuli, with latencies comparable to those found in V1, and are broadly tuned to stimulus orientation and spatiotemporal frequency. However, their receptive fields are enormous and form a unique topographic map that emphasizes the far periphery of the visual field. These results suggest a specialized circuit through which stimuli in peripheral vision can bypass the elaborate hierarchy of extrastriate visual areas and rapidly elicit coordinated motor and cognitive responses across multiple brain systems.
Animals, Brain Mapping, Callitrichinae, Limbic System, Visual Pathways, Visual Perception
null
22,704,994
2012-09-28
2022-04-08
1872-7786
Chemico-biological interactions
Curcumin encapsulated in chitosan nanoparticles: a novel strategy for the treatment of arsenic toxicity.
Yadav Abhishek, Lomash Vinay, Samim M, Flora Swaran J S
eng
null
Journal Article
Biogenic Amines, Reactive Oxygen Species, Thiobarbituric Acid Reactive Substances, Glutathione, Curcumin, Arsenic
IM
22704994, S0009-2797(12)00099-3, 10.1016/j.cbi.2012.05.011
Water-soluble nanoparticles of curcumin were synthesized, characterized and applied as a stable detoxifying agent for arsenic poisoning. Chitosan nanoparticles of less than 50 nm in diameter containing curcumin were prepared. The particles were characterized by TEM, DLS and FT-IR. The therapeutic efficacy of the encapsulated curcumin nanoparticles (ECNPs) against arsenic-induced toxicity in rats was investigated. Sodium arsenite (2mg/kg) and ECNPs (1.5 or 15 mg/kg) were orally administered to male Wistar rats for 4 weeks to evaluate the therapeutic potential of ECNPs in blood and soft tissues. Arsenic significantly decreased blood δ-aminolevulinic acid dehydratase (δ-ALAD) activity, reduced glutathione (GSH) and increased blood reactive oxygen species (ROS). These changes were accompanied by increases in hepatic total ROS, oxidized glutathione, and thiobarbituric acid-reactive substance levels. By contrast, hepatic GSH, superoxide dismutase and catalase activities significantly decreased on arsenic exposure, indicative of oxidative stress. Brain biogenic amines (dopamine, norepinephrine and 5-hydroxytryptamine) levels also showed significant changes on arsenic exposure. Co-administration of ECNPs provided pronounced beneficial effects on the adverse changes in oxidative stress parameters induced by arsenic. The results indicate that ECNPs have better antioxidant and chelating potential (even at the lower dose of 1.5 mg/kg) compared to free curcumin at 15 mg/kg. The significant neurochemical and immunohistochemical protection afforded by ECNPs indicates their neuroprotective efficacy. The formulation provides a novel therapeutic regime for preventing arsenic toxicity.
Animals, Arsenic, Arsenic Poisoning, Biogenic Amines, Brain, Curcumin, Glutathione, Lipid Peroxidation, Liver, Male, Nanoparticles, Oxidative Stress, Rats, Rats, Wistar, Reactive Oxygen Species, Thiobarbituric Acid Reactive Substances, Tissue Distribution
null
22,704,995
2012-09-28
2013-11-21
1872-7786
Chemico-biological interactions
Anticancer effect of tert-butyl-2(4,5-dihydrogen-4,4,5,5-tetramethyl-3-O-1H-imidazole-3-cationic-1-oxyl-2)-pyrrolidine-1-carboxylic ester on human hepatoma HepG2 cell line.
Guo Juan, Zhang Yanjun, Zhang Jie, Liang Jun, Zeng Lihua, Guo Guozhen
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Antineoplastic Agents, BAX protein, human, Imidazoles, Pyrrolidines, Reactive Oxygen Species, bcl-2-Associated X Protein, tert-butyl-2-(4,5-dihydrogen-4,4,5,5-tetramethyl-3-O-1H-imidazole-3-cationic-1-oxyl-2-pyrrolidine-1-carboxylic ester), Malondialdehyde, Glutathione
IM
22704995, S0009-2797(12)00100-7, 10.1016/j.cbi.2012.06.001
Tert-butyl-2(4,5-dihydrogen-4,4,5,5-tetramethyl-3-O-1H-imidazole-3-cationic-1-oxyl-2)-pyrrolidine-1-carboxylic ester (L-NNP) is a stable nitroxyl nitroxide radical, which have displayed cytotoxicity on human breast cancer MCF-7 and MDA-MB-231 cell lines. In the present study, we investigated the selective cytotoxicity of L-NNP on isogenetic human hepatoma HepG2 and normal L-02 cell lines. Cell growth inhibition, intracellular reactive oxygen species production, the mitochondrial membrane potential loss, malondialdehyde generation and glutathione levels were analyzed. The expression of Bax, Bcl-2 and NF-κBp65 proteins was also examined. The anticancer activity was evaluated in a HepG2 cell xenograft nude mice model. The results showed that 10, 20, 40 μg/ml L-NNP exposure for 48 h caused 52%, 82% and 91% cell growth inhibition of HepG2 cells, compared with 5%, 10% and 15% that of L-02 cells (p < 0.01). Concentrations of 10, 20, 40 μg/ml L-NNP induced cell death by increasing the generation of intracellular reactive oxygen species and MDA, by depolarizing the mitochondrial membrane potential, and by decreasing intracellular GSH levels in HepG2 cells. Western blot assay showed that Bax, Bcl-2 and NF-κBp65 might be implicated in L-NNP-induced selective HepG2 cell death. L-NNP was also found to inhibit HepG2 hepatoma growth and extend the life span of nude mice model (p < 0.01). The pretreatment and co-treatment of 10 mM N-acetyl-cysteine alleviated L-NNP exposure induced intracellular reactive oxygen species increase and cell growth inhibition demonstrated that L-NNP exhibited neoplasm-selective cytotoxicity and pro-apoptotic activities via reactive oxygen species mediated oxidative damage in HepG2 cells. It might be promising for developing a new class of anticancer agent for liver cancer.
Animals, Antineoplastic Agents, Carcinoma, Hepatocellular, Cell Line, Cell Proliferation, Drug Screening Assays, Antitumor, Glutathione, Hep G2 Cells, Humans, Imidazoles, Male, Malondialdehyde, Membrane Potential, Mitochondrial, Mice, Mice, Inbred BALB C, Pyrrolidines, Reactive Oxygen Species, Xenograft Model Antitumor Assays, bcl-2-Associated X Protein
null
22,704,997
2012-12-20
2012-07-30
1525-5069
Epilepsy & behavior : E&B
Charles Dickens (1812-1870) and epilepsy.
Larner A J
eng
null
Biography, Case Reports, Historical Article, Journal Article
null
IM
22704997, S1525-5050(12)00359-9, 10.1016/j.yebeh.2012.05.006
To coincide with the bicentenary of the birth of Charles Dickens (1812-1870), accounts of epilepsy found in his novels and journalism have been collated and analyzed. From these, it may be inferred that Dickens was clearly aware of the difference between epilepsy and syncope and recognized different types of epilepsy and that seizures could be fatal. Speculations that Dickens himself suffered from epilepsy are not corroborated. Dickens's novelistic construction of epilepsy as a marker of criminality, as in the characters of Monks in Oliver Twist and Bradley Headstone in Our Mutual Friend, and perhaps of mental abnormality, was in keeping with conventional contemporary views of epilepsy, but his journalistic descriptions of individuals with epilepsy confined in the workhouse system indicate an awareness of the inadequacy of their care.
Epilepsy, History, 19th Century, Humans, Journalism, Literature, Modern, Male, Middle Aged
null
22,704,996
2012-12-07
2022-04-10
1573-2509
Schizophrenia research
Determinants of treatment satisfaction of schizophrenia patients: results from the ESPASS study.
Nordon Clementine, Rouillon Frederic, Barry Caroline, Gasquet Isabelle, Falissard Bruno
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Antipsychotic Agents
IM
22704996, S0920-9964(12)00317-9, 10.1016/j.schres.2012.05.024
Knowing the determinants of treatment satisfaction can provide better understanding of patient expectations in schizophrenia. The aim of this study was to determine which treatment-related factors were associated with treatment satisfaction, independently of patient-related or illness-related factors, in schizophrenia patients.
Adolescent, Adult, Antipsychotic Agents, Cross-Sectional Studies, Female, Follow-Up Studies, France, Humans, Male, Patient Satisfaction, Psychiatric Status Rating Scales, Psychometrics, Retrospective Studies, Schizophrenia, Schizophrenic Psychology, Time Factors, Treatment Outcome, Young Adult
null
22,705,000
2012-12-07
2012-07-30
1768-3254
European journal of medicinal chemistry
Synthesis and in vitro stability of nucleoside 5'-phosphonate derivatives.
Vertuani Silvia, Baldisserotto Anna, Varani Katia, Borea Pier Andrea, De Marcos Maria Cruz Bonache, Ferraro Luca, Manfredini Stefano, Dalpiaz Alessandro
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Nucleosides, Organophosphonates, Platelet Aggregation Inhibitors
IM
22705000, S0223-5234(12)00298-X, 10.1016/j.ejmech.2012.04.045
Nucleoside derivatives are largely synthesized and tested to investigate their influence on platelet aggregation. It's well known that P2Y receptors play an important role in the regulation of platelet function and, as consequence, in controlling atherothrombotic events. The research of compounds that antagonize P2Y(1) and, in particular, P2Y(12) receptors is of great interest in the aim to obtain platelet aggregation inhibitors that are effective in the prevention and treatment of arterial thrombosis. In this study we present the synthesis and in vitro metabolic stability in human blood and rat liver homogenate of a new class of nucleoside derivatives, in particular 5'-phosphonate adenosine, inosine, guanosine and thioadenosine analogues also modified at the ribose moiety. On the basis of the results obtained we can hypothesize compounds 4 and 18 to have in vivo a relatively high stability.
Animals, Chemistry Techniques, Synthetic, Drug Stability, Humans, Liver, Male, Nucleosides, Organophosphonates, Platelet Aggregation Inhibitors, Rats, Rats, Wistar
null
22,704,998
2012-12-20
2015-11-19
1525-5069
Epilepsy & behavior : E&B
Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: an EEG wavelet analysis and retrograde neuronal tracer approach.
Rossetti Franco, Rodrigues Marcelo Cairrão Araújo, Marroni Simone S, Fernandes Artur, Foresti Maira Licia, Romcy-Pereira Rodrigo N, de Araújo Dráulio Barros, Garcia-Cairasco Norberto
eng
null
Journal Article, Research Support, Non-U.S. Gov't
2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt, GABA Agents, Stilbamidines, Muscimol, gamma-Aminobutyric Acid, Bicuculline
IM
22704998, S1525-5050(12)00341-1, 10.1016/j.yebeh.2012.04.133
The role of the substantia nigra pars reticulata (SNPr) and superior colliculus (SC) network in rat strains susceptible to audiogenic seizures still remain underexplored in epileptology. In a previous study from our laboratory, the GABAergic drugs bicuculline (BIC) and muscimol (MUS) were microinjected into the deep layers of either the anterior SC (aSC) or the posterior SC (pSC) in animals of the Wistar audiogenic rat (WAR) strain submitted to acoustic stimulation, in which simultaneous electroencephalographic (EEG) recording of the aSC, pSC, SNPr and striatum was performed. Only MUS microinjected into the pSC blocked audiogenic seizures. In the present study, we expanded upon these previous results using the retrograde tracer Fluorogold (FG) microinjected into the aSC and pSC in conjunction with quantitative EEG analysis (wavelet transform), in the search for mechanisms associated with the susceptibility of this inbred strain to acoustic stimulation. Our hypothesis was that the WAR strain would have different connectivity between specific subareas of the superior colliculus and the SNPr when compared with resistant Wistar animals and that these connections would lead to altered behavior of this network during audiogenic seizures. Wavelet analysis showed that the only treatment with an anticonvulsant effect was MUS microinjected into the pSC region, and this treatment induced a sustained oscillation in the theta band only in the SNPr and in the pSC. These data suggest that in WAR animals, there are at least two subcortical loops and that the one involved in audiogenic seizure susceptibility appears to be the pSC-SNPr circuit. We also found that WARs presented an increase in the number of FG+ projections from the posterior SNPr to both the aSC and pSC (primarily to the pSC), with both acting as proconvulsant nuclei when compared with Wistar rats. We concluded that these two different subcortical loops within the basal ganglia are probably a consequence of the WAR genetic background.
Acoustic Stimulation, Animals, Behavior, Animal, Bicuculline, Brain Waves, Disease Models, Animal, Electric Stimulation, Epilepsy, Reflex, GABA Agents, Male, Microinjections, Muscimol, Neural Pathways, Rats, Rats, Mutant Strains, Rats, Wistar, Stilbamidines, Substantia Nigra, Superior Colliculi, gamma-Aminobutyric Acid
null
22,705,001
2012-12-07
2022-03-16
1768-3254
European journal of medicinal chemistry
Withaferin A-related steroids from Withania aristata exhibit potent antiproliferative activity by inducing apoptosis in human tumor cells.
Llanos Gabriel G, Araujo Liliana M, Jiménez Ignacio A, Moujir Laila M, Bazzocchi Isabel L
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Antineoplastic Agents, Plant Extracts, Withanolides, Caspase 3, withaferin A
IM
22705001, S0223-5234(12)00343-1, 10.1016/j.ejmech.2012.05.032
Six new withanolides (1-6) along with eleven known ones (7-17) were isolated from the leaves of Withania aristata. Their structures were elucidated on the basis of spectroscopic analysis, including 1D and 2D NMR techniques. Semisynthesis of the minority metabolites 7 and 15 from compounds 6 and 9, respectively, as starting material, was performed. The isolated compounds as well as three derivatives (7a, 9a and 9b) of withaferin A were evaluated for cytotoxicity against HeLa (carcinoma of the cervix), A-549 (lung carcinoma) and MCF-7 (breast adenocarcinoma) human cancer cell lines, and against normal Vero cells (African green monkey kidney). Five compounds from this series (8, 9a, 9b, 11 and 13) exhibited potent antiproliferative effects on the tumor cells, even higher than the well known anticancer agent, withaferin A (9). Phosphatidylserine externalization, chromatin condensation, and caspase-3 activation clearly indicated apoptosis as a mechanism of action. The structure-activity relationship revealed valuable information on the pharmacophore for withanolide-type compounds.
Animals, Antineoplastic Agents, Apoptosis, Caspase 3, Cell Line, Tumor, Chlorocebus aethiops, Humans, Inhibitory Concentration 50, Plant Extracts, Structure-Activity Relationship, Vero Cells, Withania, Withanolides
null
22,704,999
2012-12-07
2016-11-25
1768-3254
European journal of medicinal chemistry
3-Hydroxy-1H-quinazoline-2,4-dione derivatives as new antagonists at ionotropic glutamate receptors: molecular modeling and pharmacological studies.
Colotta Vittoria, Lenzi Ombretta, Catarzi Daniela, Varano Flavia, Squarcialupi Lucia, Costagli Chiara, Galli Alessandro, Ghelardini Carla, Pugliese Anna Maria, Maraula Giovanna, Coppi Elisabetta, Pellegrini-Giampietro Domenico E, Pedata Felicita, Sabbadin Davide, Moro Stefano
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Anticonvulsants, Quinazolinones, Receptors, AMPA, Receptors, Kainic Acid, Glucose, Oxygen
IM
22704999, S0223-5234(12)00347-9, 10.1016/j.ejmech.2012.05.036
Based on our 3-hydroxy-7-chloroquinazoline-2,4-dione derivatives, previously reported as antagonists at ionotropic glutamate receptors, we synthesized new 3-hydroxyquinazoline-2,4-diones bearing a trifluoromethyl group at the 7-position and different groups at position 6. Glycine/NMDA, AMPA and kainate receptor binding data showed that the 7-trifluoromethyl residue increased AMPA and kainate receptor affinity and selectivity, with respect to the 7-chlorine atom. Among the probed 6-substituents, the 6-(1,2,4-triazol-4-yl) group (compound 8) was the most advantageous for AMPA receptor affinity and selectivity. Derivative 8 demonstrated to be effective in decreasing neuronal damage produced by oxygen and glucose deprivation in organotypic rat hippocampal slices and also showed anticonvulsant effects in pentylenetetrazole-induced convulsions. The previously reported kainate receptor antagonist 6-(2-carboxybenzoyl)-amino-7-chloro-3-hydroxyquinazoline-2,4-dione 3 prevented the failure of neurotransmission induced by oxygen and glucose deprivation in the CA1 region of rat hippocampal slices.
Animals, Anticonvulsants, Electrophysiological Phenomena, Glucose, Hippocampus, Humans, In Vitro Techniques, Male, Mice, Molecular Docking Simulation, Oxygen, Quinazolinones, Rats, Rats, Wistar, Receptors, AMPA, Receptors, Kainic Acid, Structure-Activity Relationship, Substrate Specificity
null
22,705,002
2012-11-23
2021-12-03
1873-2747
Brain research bulletin
Anti-nociceptive effects of Tanshinone IIA (TIIA) in a rat model of complete Freund's adjuvant (CFA)-induced inflammatory pain.
Sun Shukai, Yin Yue, Yin Xin, Cao Fale, Luo Daoshu, Zhang Ting, Li Yunqing, Ni Longxing
eng
null
Journal Article
Abietanes, Analgesics, Non-Narcotic, Anti-Inflammatory Agents, Non-Steroidal, Cytokines, Drugs, Chinese Herbal, NF-kappa B, RNA, Messenger, TRPV Cation Channels, Trpv1 protein, rat, tanshinone, dan-shen root extract, Freund's Adjuvant
IM
22705002, S0361-9230(12)00128-1, 10.1016/j.brainresbull.2012.06.002
Inflammatory pain is an important clinical symptom. The levels of extracellular signal-regulated kinases (ERKs) and the levels of cytokines such as interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) play important roles in inflammatory pain. Tanshinone IIA (TIIA) is an important component of Danshen, a traditional Chinese medicine that has been commonly used to treat cardiovascular disease. In this study, we investigated the potential anti-inflammatory nociceptive effects of TIIA on complete Freund's adjuvant (CFA)-induced inflammation and inflammatory pain in rats.
Abietanes, Analgesics, Non-Narcotic, Animals, Anti-Inflammatory Agents, Non-Steroidal, Arthritis, Experimental, Cytokines, Drug Evaluation, Preclinical, Drugs, Chinese Herbal, Freund's Adjuvant, Ganglia, Spinal, Gene Expression Regulation, Hot Temperature, Hyperalgesia, Lumbosacral Region, MAP Kinase Signaling System, Male, NF-kappa B, RNA, Messenger, Random Allocation, Rats, Rats, Sprague-Dawley, Salvia miltiorrhiza, Single-Blind Method, Stress, Mechanical, TRPV Cation Channels
null
22,705,003
2013-02-05
2021-12-03
1532-8392
Human pathology
Clinicopathologic and biological analysis of PIK3CA mutation in ovarian clear cell carcinoma.
Rahman Munmun, Nakayama Kentaro, Rahman Mohammed Tanjimur, Nakayama Naomi, Ishikawa Masako, Katagiri Atsuko, Iida Kouji, Nakayama Satoru, Otsuki Yoshiro, Shih Ie-Ming, Miyazaki Kohji
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Class I Phosphatidylinositol 3-Kinases, PIK3CA protein, human, Proto-Oncogene Proteins c-akt, TOR Serine-Threonine Kinases
IM
22705003, S0046-8177(12)00091-3, 10.1016/j.humpath.2012.03.011
Somatic mutations of PIK3CA (phosphoinositide-3-kinase) have recently been shown playing an important role in the pathogenesis of ovarian clear cell carcinoma. In this study, the frequency of PIK3CA mutations and the relationship of PIK3CA mutations with clinicopathologic and biological variables were investigated in ovarian clear cell carcinomas from Japanese patients. Mutational analysis of PIK3CA was performed in 56 primary ovarian clear cell carcinomas from Japanese women. The relationship of these mutations with various clinicopathologic and biological variables (phosphorylated AKT and phosphorylated mTOR (mammalian target of rapamycin) expression by immunohistochemistry) was determined. To clarify the roles of PI3K/AKT activation in ovarian clear cell carcinomas harboring PIK3CA mutations, we inactivated the PI3K/AKT/mTOR pathway in ovarian carcinoma cells with LY294002, temsirolimus and NVP-BEZ235. Missense mutations of PIK3CA were found in 16 (28.6%) of 56 ovarian clear cell carcinomas, but no mutation was found in 15 ovarian high-grade serous carcinomas. PIK3CA mutations were significantly associated with a favorable overall survival of patients with ovarian clear cell carcinoma (P < .05). There was no significant association between PIK3CA mutations and phosphorylated AKT or phosphorylated mTOR immunointensity status. No relationship was found between PIK3CA mutation status and sensitivity to PI3K/AKT/mTOR inhibitors in ovarian clear cell carcinoma cells. No association of PIK3CA mutations was found between positive phosphorylated AKT and positive phosphorylated mTOR, which suggests that the PI3K/AKT/mTOR pathway may be activated by other molecular mechanisms. Although PIK3CA mutations were associated with a more favorable prognosis, they did not predict the sensitivity of ovarian clear cell carcinoma cells to PI3K/AKT/mTOR inhibitors.
Adenocarcinoma, Clear Cell, Adult, Aged, 80 and over, Cell Line, Tumor, Class I Phosphatidylinositol 3-Kinases, Female, Humans, Middle Aged, Mutation, Ovarian Neoplasms, Phosphatidylinositol 3-Kinases, Phosphorylation, Prognosis, Proto-Oncogene Proteins c-akt, TOR Serine-Threonine Kinases
null
22,705,004
2013-02-05
2017-11-16
1532-8392
Human pathology
Mucinous breast carcinomas lack PIK3CA and AKT1 mutations.
Kehr Elizabeth L, Jorns Julie M, Ang Daphne, Warrick Andrea, Neff Tanaya, Degnin Michelle, Lewis Rebecca, Beadling Carol, Corless Christopher L, Troxell Megan L
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Phosphatidylinositol 3-Kinases, Class I Phosphatidylinositol 3-Kinases, PIK3CA protein, human, Receptor, ErbB-2, AKT1 protein, human, Proto-Oncogene Proteins c-akt
IM
22705004, S0046-8177(12)00092-5, 10.1016/j.humpath.2012.03.012
Activating point mutations in the phosphatidylinositol-3-kinase catalytic subunit (PIK3CA) are among the most common molecular defects in invasive breast cancer. Point mutations in the downstream kinase AKT1 are seen in a minority of carcinomas. These mutations are found preferentially in estrogen receptor-positive and Her2-positive breast carcinomas; however, special morphologic types of breast cancer have not been well studied. Twenty-nine cases of pure invasive mucinous carcinoma and 9 cases of ductal carcinoma with mucinous differentiation were screened for a panel of point mutations (>321 mutations in 30 genes) using a multiplex polymerase chain reaction panel with mass spectroscopy readout. In addition, associated ductal carcinoma in situ, hyperplasia, or columnar cell lesions were separately tested where available (25 lesions). In 3 invasive cases and 15 ductal carcinoma in situ/proliferative lesions, PIK3CA hotspot mutations were, instead, tested by direct sequencing. No point mutations were identified in invasive mucinous breast carcinoma. This contrasts with the 35% frequency of PIK3CA mutations in a comparative group of invasive ductal carcinomas of no special type. Interestingly, PIK3CA hotspot point mutations were identified in associated ductal carcinoma in situ (3/14) and hyperplasia (atypical ductal hyperplasia [2/3], usual ductal hyperplasia [2/3], columnar cell change [1/5]), suggesting that PIK3CA mutations may play a role in breast epithelial proliferation. This series represents the largest study, to date, of PIK3CA genotyping in mucinous carcinoma and supports the unique pathogenetics of invasive mucinous breast carcinoma.
Adenocarcinoma, Mucinous, Adult, Aged, Aged, 80 and over, Breast Neoplasms, Carcinoma, Ductal, Breast, Class I Phosphatidylinositol 3-Kinases, DNA Mutational Analysis, Female, Humans, Middle Aged, Mutation, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Receptor, ErbB-2
null
22,705,005
2013-02-05
2015-11-19
1532-8392
Human pathology
Immunohistochemical characteristics and malignant progression of hepatic cystic neoplasms in comparison with pancreatic counterparts.
Matsubara Takashi, Sato Yasunori, Sasaki Motoko, Harada Kenichi, Nomoto Kazuhiro, Tsuneyama Koichi, Nakamura Koichi, Gabata Toshifumi, Matsui Osamu, Nakanuma Yasuni
eng
null
Comparative Study, Journal Article
Biomarkers, Tumor, Keratin-7, Mucins, Amylases, Trypsin
IM
22705005, S0046-8177(12)00087-1, 10.1016/j.humpath.2012.03.007
The recent World Health Organization classification for tumors of the digestive system defined grossly and histologically hepatic mucinous cystic neoplasms and intraductal papillary neoplasms of the bile duct separately. In this study, the immunohistochemical features of intraductal papillary neoplasm of the bile duct (19 cases) and hepatic mucinous cystic neoplasm (5 cases) were characterized and compared with those of similar pancreatic lesions, intraductal papillary mucinous neoplasm of the pancreas (12 cases), and pancreatic mucinous cystic neoplasm (6 cases) and with those of other biliary cystic lesions, peribiliary cysts (10 cases). Intraductal papillary neoplasm of the bile duct and intraductal papillary mucinous neoplasm of the pancreas frequently expressed cytokeratin 7; mucin core proteins 1, 2, 5AC, and 6; trypsin; and amylase. Hepatic and pancreatic mucinous cystic neoplasms frequently expressed cytokeratin 7, mucin core proteins 1 and 5AC, estrogen receptor, progesterone receptor, trypsin, and amylase. Estrogen and progesterone receptors were expressed in the subepithelial stromal cells. The groups with intraductal papillary neoplasm of the bile duct and intraductal papillary mucinous neoplasm of the pancreas were different from the groups with hepatic and pancreatic mucinous cystic neoplasm with respect to several phenotypes reflecting gastric and intestinal metaplasia and also the lack of expression of estrogen and progesterone receptors. The Ki-67 and p53 labeling indexes increased significantly with the malignant progression of intraductal papillary neoplasm of the bile duct and intraductal papillary mucinous neoplasm of the pancreas. The p16 labeling index decreased and EZH2 labeling index increased significantly with the malignant progression of intraductal papillary neoplasm of the bile duct and intraductal papillary mucinous neoplasm of the pancreas. In conclusion, intraductal papillary neoplasm of the bile duct and hepatic mucinous cystic neoplasm might be regarded as biliary counterparts of intraductal papillary mucinous neoplasm of the pancreas and pancreatic mucinous cystic neoplasm, respectively, and the mucinous cystic neoplasm and intraductal papillary neoplasm groups differed from each other. Labeling indexes of Ki-67, p53, p16, and EZH2 were comparable in intraductal papillary neoplasm of the bile duct and intraductal papillary mucinous neoplasm of the pancreas along with their malignant progression, suggesting a common carcinogenic process of the tumors.
Amylases, Biomarkers, Tumor, Disease Progression, Female, Humans, Immunohistochemistry, Keratin-7, Liver Neoplasms, Male, Mucins, Neoplasms, Cystic, Mucinous, and Serous, Pancreatic Neoplasms, Trypsin
null
22,705,007
2012-11-06
2022-12-07
1528-0012
Gastroenterology
Levels of gemcitabine transport and metabolism proteins predict survival times of patients treated with gemcitabine for pancreatic adenocarcinoma.
Maréchal Raphaël, Bachet Jean-Baptiste, Mackey John R, Dalban Cécile, Demetter Pieter, Graham Kathryn, Couvelard Anne, Svrcek Magali, Bardier-Dupas Armelle, Hammel Pascal, Sauvanet Alain, Louvet Christophe, Paye François, Rougier Philippe, Penna Christophe, André Thierry, Dumontet Charles, Cass Carol E, Jordheim Lars Petter, Matera Eva-Laure, Closset Jean, Salmon Isabelle, Devière Jacques, Emile Jean-François, Van Laethem Jean-Luc
eng
null
Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't
Antimetabolites, Antineoplastic, Equilibrative Nucleoside Transporter 1, SLC29A1 protein, human, Tumor Suppressor Proteins, Deoxycytidine, RRM1 protein, human, Ribonucleoside Diphosphate Reductase, Deoxycytidine Kinase, Gemcitabine
IM
22705007, S0016-5085(12)00822-0, 10.1053/j.gastro.2012.06.006
Patients who undergo surgery for pancreatic ductal adenocarcinoma (PDAC) frequently receive adjuvant gemcitabine chemotherapy. Key determinants of gemcitabine cytotoxicity include the activities of the human equilibrative nucleoside transporter 1 (hENT1), deoxycytidine kinase (dCK), and ribonucleotide reductase subunit 1 (RRM1). We investigated whether tumor levels of these proteins were associated with efficacy of gemcitabine therapy following surgery.
Antimetabolites, Antineoplastic, Biological Transport, Biotransformation, Carcinoma, Pancreatic Ductal, Chemotherapy, Adjuvant, Chi-Square Distribution, Deoxycytidine, Deoxycytidine Kinase, Equilibrative Nucleoside Transporter 1, Female, France, Humans, Immunohistochemistry, Kaplan-Meier Estimate, Male, Multivariate Analysis, Pancreatectomy, Pancreatic Neoplasms, Proportional Hazards Models, Retrospective Studies, Ribonucleoside Diphosphate Reductase, Risk Assessment, Risk Factors, Time Factors, Tissue Array Analysis, Treatment Outcome, Tumor Suppressor Proteins, Gemcitabine
null
22,705,006
2012-10-01
2022-03-30
1528-0012
Gastroenterology
A liver full of JNK: signaling in regulation of cell function and disease pathogenesis, and clinical approaches.
Seki Ekihiro, Brenner David A, Karin Michael
eng
R01AA020172 (NIAAA NIH HHS, United States); R01 AA020172 (NIAAA NIH HHS, United States); R01GM041804 (NIGMS NIH HHS, United States); P42ES010337 (NIEHS NIH HHS, United States); P42 ES010337 (NIEHS NIH HHS, United States); R01 DK085252 (NIDDK NIH HHS, United States); R01DK085252 (NIDDK NIH HHS, United States); R01 GM041804 (NIGMS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Review
Analgesics, Non-Narcotic, Biomarkers, Protein Kinase Inhibitors, Acetaminophen, JNK Mitogen-Activated Protein Kinases
IM
22705006, S0016-5085(12)00820-7, 10.1053/j.gastro.2012.06.004, PMC3523093, NIHMS393916, 19053047, 9755232, 17983584, 15793284, 16955144, 21953113, 15316358, 18393321, 15486293, 20478530, 17133482, 11148566, 21844199, 16912279, 11713531, 19041150, 18337250, 19243309, 17050683, 18700144, 8945519, 20080763, 20303879, 20683948, 20144759, 18182393, 20043871, 16374858, 19167327, 18691550, 19418558, 19220657, 17940019, 17468757, 19060338, 15885356, 20637208, 18922779, 18497693, 17158707, 16818881, 15989949, 19524512, 21145844, 19734538, 21406557, 11875461, 15537542, 19106147, 12887920, 21147505, 17366662, 19841069, 20354226, 17292824, 2114348, 17570222, 11927562, 15987843, 19638343, 22128176, 16831600, 21703174, 20080940, 12736142, 11679966, 1415718, 14766793, 21654829, 16571730, 19249395, 19900593, 18773087, 19629069, 19667931, 20141834, 17952090, 15766528, 21488081, 11283855, 10352021, 19524513, 21333379, 14614859, 19549522, 17300814, 19945406, 19782079, 21270260, 21962514, 16469705, 22244937, 12447443, 19498109, 11915022, 20679483, 17470478, 21216243, 19033664, 16807293, 8650258, 17570221, 15350216, 12711737, 11057897, 15545623, 14767992, 15329734, 12668975, 19883619, 18671679, 12553907, 17560373, 18382767, 17906064, 7860764
c-Jun-N-terminal kinase (JNK) is a mitogen-activated protein kinase family member that is activated by diverse stimuli, including cytokines (such as tumor necrosis factor and interleukin-1), reactive oxygen species (ROS), pathogens, toxins, drugs, endoplasmic reticulum stress, free fatty acids, and metabolic changes. Upon activation, JNK induces multiple biologic events through the transcription factor activator protein-1 and transcription-independent control of effector molecules. JNK isozymes regulate cell death and survival, differentiation, proliferation, ROS accumulation, metabolism, insulin signaling, and carcinogenesis in the liver. The biologic functions of JNK are isoform, cell type, and context dependent. Recent studies using genetically engineered mice showed that loss or hyperactivation of the JNK pathway contributes to the development of inflammation, fibrosis, cancer growth, and metabolic diseases that include obesity, hepatic steatosis, and insulin resistance. We review the functions and pathways of JNK in liver physiology and pathology and discuss findings from preclinical studies with JNK inhibitors.
Acetaminophen, Analgesics, Non-Narcotic, Animals, Biomarkers, Carcinoma, Hepatocellular, Cell Death, Chemical and Drug Induced Liver Injury, Fatty Liver, Humans, JNK Mitogen-Activated Protein Kinases, Liver, Liver Diseases, Liver Neoplasms, Liver Regeneration, Liver Transplantation, MAP Kinase Signaling System, Metabolic Syndrome, Mice, Primary Graft Dysfunction, Protein Kinase Inhibitors
null
22,705,008
2012-12-17
2024-12-15
1528-0012
Gastroenterology
Successful vaccination induces multifunctional memory T-cell precursors associated with early control of hepatitis C virus.
Park Su-Hyung, Shin Eui-Cheol, Capone Stefania, Caggiari Laura, De Re Valli, Nicosia Alfredo, Folgori Antonella, Rehermann Barbara
eng
ZIA DK054508-12 (Intramural NIH HHS, United States); ZIA DK054508-13 (Intramural NIH HHS, United States); Z01 DK054508-11 (Intramural NIH HHS, United States); Z01 DK054508 (Intramural NIH HHS, United States); Z01 DK054508-10 (Intramural NIH HHS, United States)
Comparative Study, Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't
B7-H1 Antigen, DNA, Viral, Interleukin-7 Receptor alpha Subunit, Programmed Cell Death 1 Receptor, RNA, Messenger, Tumor Necrosis Factor-alpha, Viral Hepatitis Vaccines, Interferon-gamma, 2',5'-Oligoadenylate Synthetase
IM
22705008, S0016-5085(12)00821-9, 10.1053/j.gastro.2012.06.005, PMC3458177, NIHMS389706, 17039255, 16876901, 8671665, 17485666, 17258731, 16941702, 12663785, 14625547, 14576438, 16462801, 19587449, 21604986, 10229187, 12810686, 17326154, 18981091, 21699897, 18667501, 18753204, 12021342, 14671100, 14722311, 17058243, 17376899, 12615904, 16729320, 11714747, 18667516, 10790425, 16698427, 12441397, 16799989, 7519785, 15220475, 10802716
T cells are an important component for development of a vaccine against hepatitis C virus (HCV), but little is known about the features of successful vaccine-induced T cells.
2',5'-Oligoadenylate Synthetase, Analysis of Variance, Animals, B7-H1 Antigen, CD8-Positive T-Lymphocytes, DNA, Viral, Hepacivirus, Hepatitis C, Immunologic Memory, Interferon-gamma, Interleukin-7 Receptor alpha Subunit, Liver, Mice, Mice, Inbred C57BL, Pan troglodytes, Phenotype, Precursor Cells, T-Lymphoid, Programmed Cell Death 1 Receptor, RNA, Messenger, Statistics, Nonparametric, Tumor Necrosis Factor-alpha, Vaccination, Viral Hepatitis Vaccines, Viral Load
null
22,705,010
2012-12-13
2012-07-30
1873-569X
Journal of dermatological science
Tyrosinase-related protein1 in mouse melanocytes at early embryonic stage.
Kawakami Tamihiro, Soma Yoshinao
eng
null
Letter, Research Support, Non-U.S. Gov't
Membrane Glycoproteins, Microphthalmia-Associated Transcription Factor, Mitf protein, mouse, Oxidoreductases, Tyrp1 protein, mouse, Monophenol Monooxygenase
IM
22705010, S0923-1811(12)00175-2, 10.1016/j.jdermsci.2012.05.004
null
Animals, Female, Gene Expression Regulation, Developmental, Male, Melanocytes, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Microphthalmia-Associated Transcription Factor, Monophenol Monooxygenase, Neural Crest, Oxidoreductases, Skin
null
22,705,009
2012-11-06
2017-07-10
1528-0012
Gastroenterology
Identification of PTK6, via RNA sequencing analysis, as a suppressor of esophageal squamous cell carcinoma.
Ma Stephanie, Bao Jessie Y J, Kwan Pak Shing, Chan Yuen Piu, Tong Carol M, Fu Li, Zhang Na, Tong Amy H Y, Qin Yan-Ru, Tsao Sai Wah, Chan Kwok Wah, Lok Si, Guan Xin-Yuan
eng
null
Journal Article, Research Support, Non-U.S. Gov't
CTNNB1 protein, human, Histones, Neoplasm Proteins, RNA, Messenger, Tumor Suppressor Proteins, beta Catenin, Protein-Tyrosine Kinases, PTK6 protein, human, GSK3B protein, human, Glycogen Synthase Kinase 3 beta, Gsk3b protein, mouse, Proto-Oncogene Proteins c-akt, Glycogen Synthase Kinase 3
IM
22705009, S0016-5085(12)00823-2, 10.1053/j.gastro.2012.06.007
Esophageal squamous cell carcinoma (ESCC) is the most commonly observed histologic subtype of esophageal cancer. ESCC is believed to develop via accumulation of numerous genetic alterations, including inactivation of tumor suppressor genes and activation of oncogenes. We searched for transcripts that were altered in human ESCC samples compared with nontumor tissues.
Acetylation, Adult, Animals, Carcinoma, Squamous Cell, Cell Line, Tumor, Cell Movement, Cell Proliferation, DNA Methylation, Epigenesis, Genetic, Esophageal Neoplasms, Extracellular Matrix, Female, G1 Phase Cell Cycle Checkpoints, Gene Expression Profiling, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Glycogen Synthase Kinase 3, Glycogen Synthase Kinase 3 beta, Histones, Humans, Male, Mice, Mice, Nude, Middle Aged, Neoplasm Invasiveness, Neoplasm Proteins, Phosphorylation, Promoter Regions, Genetic, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-akt, RNA Interference, RNA, Messenger, Sequence Analysis, RNA, Signal Transduction, Transcription, Genetic, Transfection, Tumor Suppressor Proteins, beta Catenin
null
22,705,011
2013-07-22
2012-12-03
1878-1519
Respiratory physiology & neurobiology
Postnatal maturation of breathing stability and loop gain: the role of carotid chemoreceptor development.
Edwards Bradley A, Sands Scott A, Berger Philip J
eng
null
Journal Article, Research Support, Non-U.S. Gov't, Review
null
IM
22705011, S1569-9048(12)00144-9, 10.1016/j.resp.2012.06.003
Any general model of respiratory control must explain a puzzling array of breathing patterns that are observed during the course of a lifetime. Particular challenges are to understand why periodic breathing is rarely seen in the first few days after birth, reaches a peak at 2-4 weeks postnatal age, and disappears by 6 months, why it is prevalent in preterm infants, and why it reappears in adults at altitude or with heart failure. In this review we use the concept of loop gain to obtain quantitative insight into the genesis of unstable breathing patterns with a particular focus on how changes in carotid body function could underlie the age-related dependence of periodic breathing.
Carotid Body, Humans, Infant, Newborn, Respiration, Respiratory Mechanics, Respiratory System
null
22,705,012
2013-04-15
2013-11-21
1878-1519
Respiratory physiology & neurobiology
Carbon monoxide exposure in the urban environment: an insidious foe for the heart?
Reboul C, Thireau J, Meyer G, André L, Obert P, Cazorla O, Richard S
eng
null
Journal Article, Review
Air Pollutants, Carbon Monoxide
IM
22705012, S1569-9048(12)00151-6, 10.1016/j.resp.2012.06.010
Since Claude Bernard first demonstrated in the 19th century that carbon monoxide (CO) poisoning occurs through hemoglobin binding, CO has proven to be more than simply a toxic gas, and to possess complex biological properties. In this review, we highlight the dual nature of CO in cardiovascular function, from endogenous and therapeutic properties to harmful aspects. Focussing on exposure to low environmental CO levels, the most common but least studied form of exposure, we summarize the pathophysiological effects of CO in vivo and in vitro, from cardiac disorders to phenotypic remodelling of cardiomyocytes, based on clinical observations and experimental studies. While acute exposure to low CO levels is considered beneficial and cardioprotective, prolonged exposure appears deleterious, mainly due to alterations in redox status, ion homeostasis, intracellular Ca(2+) handling, and sympathovagal balance. We emphasize that, despite its fascinating therapeutic potential at low levels, regular exposure to CO may have significant consequences on cardiovascular health and must be considered a cardiovascular risk factor.
Air Pollutants, Animals, Carbon Monoxide, Electrophysiological Phenomena, Environmental Exposure, Heart Diseases, Humans, Urban Population
null
22,705,014
2013-05-29
2012-11-16
1878-1780
Diabetes & metabolism
Type 1 diabetes: dealing with physical activity.
Franc S, Dardari D, Biedzinski M, Requeda E, Canipel L, Hochberg G, Boucherie B, Charpentier G
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Blood Glucose, Hypoglycemic Agents, Insulin
IM
22705014, S1262-3636(12)00076-6, 10.1016/j.diabet.2012.03.009
For patients with type 1 diabetes (T1D) using multiple insulin injections (MII), there are currently no guidelines for insulin dose adjustments in the event of physical activity (PA) and no simple algorithms that can be applied directly. Thus, the objective of this study was to assess the relevance of simple algorithms based on assessments of PA intensity by T1D patients themselves.
Adult, Algorithms, Blood Glucose, Diabetes Mellitus, Type 1, Drug Administration Schedule, Exercise, Female, Humans, Hypoglycemia, Hypoglycemic Agents, Insulin, Male, Postprandial Period, Time Factors
null
22,705,013
2013-03-17
2022-03-09
1878-1519
Respiratory physiology & neurobiology
Recovery of inspiratory intercostal muscle activity following high cervical hemisection.
Dougherty B J, Lee K Z, Gonzalez-Rothi E J, Lane M A, Reier P J, Fuller D D
eng
1R01-NS-054025 (NINDS NIH HHS, United States); T-32 HD043730 (NICHD NIH HHS, United States); R21 HL104294 (NHLBI NIH HHS, United States); 1F31NS063659-01A2 (NINDS NIH HHS, United States); 1R21-HL104294-01 (NHLBI NIH HHS, United States); F31 NS063659 (NINDS NIH HHS, United States); R01 NS054025 (NINDS NIH HHS, United States); T32 HD043730 (NICHD NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural
null
IM
22705013, S1569-9048(12)00147-4, 10.1016/j.resp.2012.06.006, PMC4288928, NIHMS429428, 18308305, 18775573, 19150658, 19560562, 11160996, 14764434, 6707715, 10963770, 12657710, 4078753, 21106900, 15871925, 7836225, 20043908, 12486024, 12228053, 8594024, 18924146, 3598633, 3411478, 15037862, 7932235, 17974589, 15788709, 16556657, 3956619, 16647702, 6747851, 12531916, 11606656, 8101520, 15531560, 9490849, 14699417, 9379420, 19698805, 22033536, 19539790, 16269524
Anatomical and neurophysiological evidence indicates that thoracic interneurons can serve a commissural function and activate contralateral motoneurons. Accordingly, we hypothesized that respiratory-related intercostal (IC) muscle electromyogram (EMG) activity would be only modestly impaired by a unilateral cervical spinal cord injury. Inspiratory tidal volume (VT) was recorded using pneumotachography and EMG activity was recorded bilaterally from the 1st to 2nd intercostal space in anesthetized, spontaneously breathing rats. Studies were conducted at 1-3 days, 2 wks or 8 wks following C2 spinal cord hemisection (C2HS). Data were collected during baseline breathing and a brief respiratory challenge (7% CO(2)). A substantial reduction in inspiratory intercostal EMG bursting ipsilateral to the lesion was observed at 1-3 days post-C2HS. However, a time-dependent return of activity occurred such that by 2 wks post-injury inspiratory intercostal EMG bursts ipsilateral to the lesion were similar to age-matched, uninjured controls. The increases in ipsilateral intercostal EMG activity occurred in parallel with increases in VT following the injury (R=0.55; P<0.001). We conclude that plasticity occurring within a "crossed-intercostal" circuitry enables a robust, spontaneous recovery of ipsilateral intercostal activity following C2HS in rats.
Animals, Cervical Vertebrae, Inhalation, Intercostal Muscles, Male, Neuronal Plasticity, Rats, Rats, Sprague-Dawley, Recovery of Function, Respiratory Mechanics, Spinal Cord Injuries
null
22,705,015
2012-12-03
2021-10-21
1879-3088
Trends in cell biology
Multi-tasking: nuclear transcription factors with novel roles in the mitochondria.
Szczepanek Karol, Lesnefsky Edward J, Larner Andrew C
eng
R01 AI059710 (NIAID NIH HHS, United States); R01 GM101677 (NIGMS NIH HHS, United States); R21 AI088487 (NIAID NIH HHS, United States); R01 AI059710-01 (NIAID NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Review
Transcription Factors
IM
22705015, S0962-8924(12)00084-0, 10.1016/j.tcb.2012.05.001, PMC3516366, NIHMS387756, 21715323, 18391940, 16207717, 18056423, 21036145, 21930250, 19556508, 16728594, 17460192, 12567188, 10821866, 20360684, 19411846, 18988672, 15963355, 21393861, 15867370, 21747053, 11522775, 11900485, 21980124, 12867595, 9832167, 11287411, 12839966, 20407820, 19628817, 22157762, 11950925, 12667443, 21346730, 10349835, 15347644, 10679241, 21968997, 20224768, 11927553, 19938943, 15231833, 12235142, 2176148, 14566054, 20960209, 12433922, 18279015, 8662694, 20628368, 19131594, 17268548, 21329348, 12191768, 21742773, 16051147, 16513254, 12379849, 7517985, 19614745, 18846505, 20807804, 16169904, 14963330, 17289576, 15367666, 21738764, 11733493, 19007744, 16862141, 20962592, 12628925, 15077116, 22393233
Coordinated responses between the nucleus and mitochondria are essential for the maintenance of homeostasis. For over 15 years, pools of nuclear transcription factors (TFs), such as p53 and nuclear hormone receptors, have been observed in the mitochondria. The contribution of the mitochondrial pool of these TFs to their well-defined biological actions is in some cases clear and in others not well understood. Recently, a small mitochondrial pool of the TF signal transducer and activator of transcription factor 3 (STAT3) was shown to modulate the activity of the electron transport chain (ETC). The mitochondrial function of STAT3 encompasses both its biological actions in the heart as well as its oncogenic effects. This review highlights advances in our understanding of how mitochondrial pools of nuclear TFs may influence the function of this organelle.
Animals, Cell Nucleus, Cell Respiration, Gene Expression Regulation, Humans, Mitochondria, Signal Transduction, Transcription Factors
null
22,705,017
2012-11-13
2022-03-31
1096-0260
Preventive medicine
Increased risk of chronic kidney disease among users of non-prescribed Chinese herbal medicine in Taiwan.
Hsieh Chuan-Fa, Huang Song-Lih, Chen Chien-Lung, Chen Wei-Ta, Chang Huan-Cheng, Wu Ming-Ling, Yang Chen-Chang
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Drugs, Chinese Herbal
IM
22705017, S0091-7435(12)00249-6, 10.1016/j.ypmed.2012.06.003
Taiwan has the highest incidence of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide. Chinese herbal medicine (CHM) has been linked to CKD/ESRD in Taiwan. The specific effects and frequency of CHM on the risk of CKD are unknown.
Adult, Aged, Case-Control Studies, Chronic Disease, Comorbidity, Confounding Factors, Epidemiologic, Drugs, Chinese Herbal, Exercise, Female, Health Knowledge, Attitudes, Practice, Humans, Kidney Failure, Chronic, Life Style, Male, Middle Aged, Multivariate Analysis, Proportional Hazards Models, Renal Insufficiency, Chronic, Retrospective Studies, Risk Factors, Smoking, Socioeconomic Factors, Surveys and Questionnaires, Taiwan
null
22,705,016
2013-02-08
2024-08-08
1096-0260
Preventive medicine
Parent-focused change to prevent obesity in preschoolers: results from the KAN-DO study.
Østbye Truls, Krause Katrina M, Stroo Marissa, Lovelady Cheryl A, Evenson Kelly R, Peterson Bercedis L, Bastian Lori A, Swamy Geeta K, West Deborah G, Brouwer Rebecca J N, Zucker Nancy L
eng
K23 MH070418 (NIMH NIH HHS, United States); P30 ES010126 (NIEHS NIH HHS, United States); 1-K23-MH-070-01 (NIMH NIH HHS, United States); R01-DK-07549 (NIDDK NIH HHS, United States); R01 DK075439 (NIDDK NIH HHS, United States)
Journal Article, Randomized Controlled Trial, Research Support, N.I.H., Extramural
null
IM
22705016, S0091-7435(12)00251-4, 10.1016/j.ypmed.2012.06.005, PMC3439558, NIHMS386646, 20633234, 16611394, 14702458, 20883981, 17572310, 19413705, 17467752, 9813653, 9724677, 15047683, 10799366, 21300177, 14640806, 15488428, 16864353, 16678304, 10719403, 14084769, 11817924, 12729175, 16895873, 18463121, 15466068, 17907317, 17524711, 10600433, 14717257, 9302300, 20406416, 17332205, 12055321, 15066873, 21657977, 18949660, 17391806, 11886937, 18483480, 15354047, 21424979, 14640827, 16203629, 20188297, 20534744, 16394968, 10916515
The study presents the immediate post-intervention results of Kids and Adults Now - Defeat Obesity!, a randomized controlled trial to enhance healthy lifestyle behaviors in mother-preschooler (2-5 years old) dyads in North Carolina (2007-2011). The outcomes include change from baseline in the child's diet, physical activity and weight, and in the mother's parenting behaviors, diet, physical activity, and weight.
Adult, Child, Preschool, Diet, Emotions, Exercise, Female, Humans, Male, Maternal Behavior, North Carolina, Obesity, Parenting
null
22,705,018
2012-12-11
2012-08-27
1872-6232
Early human development
Altered placental development in pregnancies resulting in sudden infant death syndrome (SIDS).
Widdows Kate, O'Malley Aiveen, O'Neill Bill, Kingdom John, Gillan John, Ansari Tahera
eng
null
Journal Article, Research Support, Non-U.S. Gov't
null
IM
22705018, S0378-3782(12)00134-X, 10.1016/j.earlhumdev.2012.05.006
Sudden infant death syndrome (SIDS) is postulated to be a developmental disorder originating during fetal life in utero. Knowledge regarding the intrauterine environment in which SIDS infants develop is, however, inadequate and how the placenta develops prior to a SIDS event has not been studied.
Adolescent, Adult, Chorionic Villi, Female, Humans, Infant, Newborn, Infant, Small for Gestational Age, Male, Placenta Diseases, Pregnancy, Sudden Infant Death, Trophoblasts
null
22,705,019
2012-11-02
2012-07-03
1464-3391
Bioorganic & medicinal chemistry
Synthesis of 6-substituted 9-methoxy-11H-indeno[1,2-c]quinoline-11-one derivatives as potential anticancer agents.
Tseng Chih-Hua, Chen Yeh-Long, Yang Chiao-Li, Cheng Chih-Mei, Han Chein-Hwa, Tzeng Cherng-Chyi
eng
null
Journal Article, Research Support, Non-U.S. Gov't
6-(3-(dimethylamino)propylamino)-9-methoxy-11H-indeno(1,2-c)quinolin-11-one, Antineoplastic Agents, Indenes, Quinolizidines, Quinolones, Poly(ADP-ribose) Polymerases, Caspase 3, Caspase 8
IM
22705019, S0968-0896(12)00409-9, 10.1016/j.bmc.2012.05.035
We have synthesized certain 6-substituted 9-methoxy-11H-indeno[1,2-c]quinolin-11-ones for antiproliferative evaluation. Results indicated that 6-alkylamine derivatives, 6-[2-(dimethylamino)ethylamino]-9-methoxy-11H-indeno[1,2-c]quinolin-11-one (5a) and its 6-[3-(dimethylamino)propylamino] derivative, 5b, were able to inhibit cells growth completely at a concentration of 100 μM while most of the 6-arylamine derivatives 6b-6h were inactive at the same concentration. Comparable mean GI(50) (drug molar concentration causing 50% cell growth inhibition) values for 5a (3.47 μM) and 5b (3.39 μM) indicated the cytotoxicity may not be affected by the length of alkyl substituents at C-6 position. Compound 5b, with a mean GI(50) value of 3.39 μM, was the most active and therefore was selected for further evaluation on its effects of H460 lung cancer cell cycle distribution. Results indicated that 5b induced cell cycle arrest in G2/M phase after 24h treatment, while the hypodiploid (sub-G0/G1 phase) cells increased. We found that H460 cell became shrinked after the treatment of 5b, indicating that apoptosis may be a mechanism by which 5b kills the cancer cells. DNA unwinding assay indicated that 5b may bind to DNA through intercalation. Our results have also demonstrated that PARP was cleaved while caspase-3 and caspase-8 activities were induced after the treatment of 5b at 10 μM for 24h. Thus, compound 5b intercalates DNA, induces cell cycle arrest at G2/M phase via cleavage of PARP, induces caspase-3 and caspase-8 activities, and consequently causes the cell death.
Antineoplastic Agents, Apoptosis, Caspase 3, Caspase 8, Cell Line, Tumor, G2 Phase Cell Cycle Checkpoints, Humans, Indenes, M Phase Cell Cycle Checkpoints, Poly(ADP-ribose) Polymerases, Quinolizidines, Quinolones
null
22,705,020
2012-11-02
2024-06-10
1464-3391
Bioorganic & medicinal chemistry
Myxobacteria versus sponge-derived alkaloids: the bengamide family identified as potent immune modulating agents by scrutiny of LC-MS/ELSD libraries.
Johnson Tyler A, Sohn Johann, Vaske Yvette M, White Kimberly N, Cohen Tanya L, Vervoort Helene C, Tenney Karen, Valeriote Frederick A, Bjeldanes Leonard F, Crews Phillip
eng
U01 TW008160 (FIC NIH HHS, United States); R01 CA047135 (NCI NIH HHS, United States); 1U01TW008160-01 (FIC NIH HHS, United States); R01 CA 47135 (NCI NIH HHS, United States); T34 GM007910 (NIGMS NIH HHS, United States)
Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.
Alkaloids, Azepines, Chemokine CCL2, Immunologic Factors, Interleukin-6, Lipopolysaccharides, NF-kappa B, Tumor Necrosis Factor-alpha, bengamide E, Nitric Oxide Synthase Type II, I-kappa B Kinase
IM
22705020, S0968-0896(12)00417-8, 10.1016/j.bmc.2012.05.043, PMC3417756, NIHMS388161, 16879738, 11809076, 15332835, 19046569, 17072334, 11438690, 12656172, 9790549, 17622130, 18657422, 8557566, 20866076, 18060028, 20179242, 11997170, 20520915, 11671930, 7568127, 17072321, 11170681, 10048565, 21465667, 11606134, 16651429, 12049530, 14534293, 22118830, 16603636, 12866845, 12039983, 16278381, 9032318, 21170424, 10602465, 18161752, 11514225, 20121114, 17159608, 11169761, 16793524, 14708018, 20030364, 17227230, 17893868, 22129061, 19755514, 20704428, 21402700, 11262120, 12469307, 18624308, 11134171, 17656313, 15013524
A nuclear factor-κB (NF-κB) luciferase assay has been employed to identify the bengamides, previously known for their anti-tumor activity, as a new class of immune modulators. A unique element of this study was that the bengamide analogs were isolated from two disparate sources, Myxococcus virescens (bacterium) and Jaspis coriacea (sponge). Comparative LC-MS/ELSD and NMR analysis facilitated the isolation of M. viriscens derived samples of bengamide E (8) and two congeners, bengamide E' (13) and F' (14) each isolated as an insperable mixture of diastereomers. Additional compounds drawn from the UC, Santa Cruz repository allowed expansion of the structure activity relationship (SAR) studies. The activity patterns observed for bengamide A (6), B (7), E (8), F (9), LAF 389 (12) and 13-14 gave rise to the following observations and conclusions. Compounds 6 and 7 display potent inhibition of NF-κB (at 80 and 90 nM, respectively) without cytotoxicity to RAW264.7 macrophage immune cells. Western blot and qPCR analysis indicated that 6 and 7 reduce the phosphorylation of IκBα and the LPS-induced expression of the pro-inflammatory cytokines/chemokines TNFα, IL-6 and MCP-1 but do not effect NO production or the expression of iNOS. These results suggest that the bengamides may serve as therapeutic leads for the treatment of diseases involving inflammation, that their anti-tumor activity can in part be attributed to their ability to serve as immune modulating agents, and that their therapeutic potential against cancer merits further consideration.
Alkaloids, Animals, Azepines, Chemokine CCL2, Chromatography, High Pressure Liquid, HCT116 Cells, Humans, I-kappa B Kinase, Immunologic Factors, Interleukin-6, Lipopolysaccharides, Macrophages, Mass Spectrometry, Mice, Myxococcales, NF-kappa B, Nitric Oxide Synthase Type II, Phosphorylation, Porifera, Tumor Necrosis Factor-alpha
null
22,705,021
2012-11-02
2018-12-01
1464-3391
Bioorganic & medicinal chemistry
Functional characterization of recombinant hyoscyamine 6β-hydroxylase from Atropa belladonna.
Li Jing, van Belkum Marco J, Vederas John C
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Oxygen Isotopes, Plant Proteins, Recombinant Proteins, Scopolamine, Mixed Function Oxygenases, hyoscyamine (6S)-dioxygenase
IM
22705021, S0968-0896(12)00416-6, 10.1016/j.bmc.2012.05.042
(-)-Hyoscyamine, the enantiomerically pure form of atropine, and its derivative scopolamine are tropane alkaloids that are extensively used in medicine. Hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), a monomeric α-ketoglutarate dependent dioxygenase, converts (-)-hyoscyamine to its 6,7-epoxy derivative, scopolamine, in two sequential steps. In this study, H6H of Atropa belladonna (AbH6H) was cloned, heterologously expressed in Escherichia coli, purified and characterized. The catalytic efficiency of AbH6H, especially for the second oxidation, was found to be low, and this may be one of the reasons why Atropa belladonna produces less scopolamine than other species in the same family. 6,7-Dehydrohyoscyamine, a potential precursor for the last step of epoxidation, was shown not to be an obligatory intermediate in the biosynthesis of scopolamine using purified AbH6H with an in vitro (18)O labeling experiment. Moreover, the nitrogen atom in the tropane ring of (-)-hyoscyamine was found to play an important role in substrate recognition.
Atropa belladonna, Kinetics, Mixed Function Oxygenases, Oxidation-Reduction, Oxygen Isotopes, Plant Proteins, Recombinant Proteins, Scopolamine, Stereoisomerism, Substrate Specificity
null
22,705,022
2012-11-02
2012-11-15
1464-3391
Bioorganic & medicinal chemistry
Synthesis, biological evaluation and molecular docking studies of 3-(1,3-diphenyl-1H-pyrazol-4-yl)-N-phenylacrylamide derivatives as inhibitors of HDAC activity.
Li Xi, Liu Jia-Lin, Yang Xian-Hui, Lu Xiang, Zhao Ting-Ting, Gong Hai-Bin, Zhu Hai-Liang
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Acrylamides, Antineoplastic Agents, Histone Deacetylase Inhibitors, N-(4-bromophenyl)-3-(3-(4-nitrophenyl)-1-phenyl-1H-pyrazol-4-yl)acrylamide, N-phenylacrylamide, Pyrazoles, pyrazole, Histone Deacetylases
IM
22705022, S0968-0896(12)00405-1, 10.1016/j.bmc.2012.05.031
In present study, a series of 3-(1,3-diphenyl-1H-pyrazol-4-yl)-N-phenylacrylamide derivatives (5a-8d) were designed, synthesized, and evaluated for HDAC inhibition and tumor cell antiproliferation. All of these compounds are reported for the first time, the chemical structures of these compounds were confirmed by means of (1)H NMR, ESI-MS and elemental analyzes. Among the compounds, compound 8c showed the most potent biological activity against HCT116 cancer cell line (IC(50) of 0.42 ± 0.02 μM for HDAC-1 and IC(50)=0.62 ± 0.02 for HCT116). Docking simulation was performed to position compound 8c into the HDAC active site to determine the probable binding model. The results of antiproliferative assay and western-blot demonstrated that compound 8c with potent inhibitory activity in tumor growth inhibition may be a potential anticancer agent against HCT116 cancer cell.
Acrylamides, Antineoplastic Agents, Binding Sites, Cell Line, Tumor, Cell Proliferation, Computer Simulation, Drug Screening Assays, Antitumor, HCT116 Cells, Histone Deacetylase Inhibitors, Histone Deacetylases, Humans, Protein Structure, Tertiary, Pyrazoles, Structure-Activity Relationship
null
22,705,024
2012-12-21
2024-01-09
1879-0356
Current opinion in plant biology
Plant pattern recognition receptor complexes at the plasma membrane.
Monaghan Jacqueline, Zipfel Cyril
eng
null
Journal Article, Research Support, Non-U.S. Gov't, Review
Membrane Proteins, Receptors, Pattern Recognition
IM
22705024, S1369-5266(12)00087-8, 10.1016/j.pbi.2012.05.006
A key feature of innate immunity is the ability to recognize and respond to potential pathogens in a highly sensitive and specific manner. In plants, the activation of pattern recognition receptors (PRRs) by pathogen-associated molecular patterns (PAMPs) elicits a defense programme known as PAMP-triggered immunity (PTI). Although only a handful of PAMP-PRR pairs have been defined, all known PRRs are modular transmembrane proteins containing ligand-binding ectodomains. It is becoming clear that PRRs do not act alone but rather function as part of multi-protein complexes at the plasma membrane. Recent studies describing the molecular interactions and protein modifications that occur between PRRs and their regulatory proteins have provided important mechanistic insight into how plants avoid infection and achieve immunity.
Cell Membrane, Disease Resistance, Host-Pathogen Interactions, Membrane Proteins, Plant Diseases, Plant Immunity, Plants, Receptors, Pattern Recognition, Signal Transduction
null
22,705,023
2012-11-02
2016-11-25
1464-3391
Bioorganic & medicinal chemistry
Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation.
Kashida Hiromu, Yamaguchi Kyohei, Hara Yuichi, Asanuma Hiroyuki
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Amino Alcohols, Butylene Glycols, Intercalating Agents, Protons, Umbelliferones, threoninol, 7-hydroxycoumarin, DNA
IM
22705023, S0968-0896(12)00439-7, 10.1016/j.bmc.2012.05.052
In this study, we synthesized a simple but efficient quencher-free molecular beacon tethering 7-hydroxycoumarin on D-threoninol based on its pK(a) change. The pK(a) of 7-hydroxycoumarin in a single strand was determined as 8.8, whereas that intercalated in the duplex was over 10. This large pK(a) shift (more than 1.2) upon hybridization could be attributed to the anionic and hydrophobic microenvironment inside the DNA duplex. Because 7-hydroxycoumarin quenches its fluorescence upon protonation, the emission intensity of the duplex at pH 8.5 was 1/15 that of the single strand. We applied this quenching mechanism to the preparation of a quencher-free molecular beacon by introducing the dye into the middle of the stem part. In the absence of the target, the stem region formed a duplex and fluorescence was quenched. However, when the target was added, the molecular beacon opened and the dye was deprotonated. As a result, the emission intensity of the molecular beacon with the target was 10 times higher than that without the target. Accordingly, a quencher-free molecular beacon utilizing the pK(a) change was successfully developed.
Amino Alcohols, Butylene Glycols, DNA, Hydrogen-Ion Concentration, Intercalating Agents, Kinetics, Nucleic Acid Denaturation, Nucleic Acid Hybridization, Protons, Umbelliferones
null
22,705,025
2013-05-20
2012-12-03
2210-741X
Clinics and research in hepatology and gastroenterology
Evaluation of preoperative predictors of development of pouchitis after ileal-pouch-anastomosis in ulcerative colitis.
Kalkan I H, Dağli Ü, Önder F O, Tunç B, Öztaş E, Ülker A, Şaşmaz N
eng
null
Journal Article
null
IM
22705025, S2210-7401(12)00131-3, 10.1016/j.clinre.2012.04.012
In this retrospective study, we aimed to evaluate preoperative predictive risk factors for development of pouchitis in the ulcerative colitis (UC) patients with ileal pouch-anal anastomosis (IPAA).
Adult, Anastomosis, Surgical, Colitis, Ulcerative, Colonic Pouches, Female, Humans, Ileum, Male, Pouchitis, Preoperative Period, Retrospective Studies, Risk Assessment
null
22,705,026
2012-09-25
2016-11-25
1879-0038
Gene
Molecular characterization and concerted evolution of two genes encoding RING-C2 type proteins in rice.
Jung Chang Gyo, Lim Sung Don, Hwang Sun-Goo, Jang Cheol Seong
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Carrier Proteins, Plant Proteins, Ubiquitin-Protein Ligases
IM
22705026, S0378-1119(12)00679-8, 10.1016/j.gene.2012.05.060
RING (Really Interesting New Gene) finger proteins are believed to play a critical role in mediating the transfer of ubiquitin to heterogeneous substrate(s). While the two canonical types, RING-H2 and RING-HC, have been well-characterized, the molecular functions of the modified types, particularly the RING-C2 types, remain elusive. We isolated two rice genes harboring the RING-C2 domain on the distal parts of rice chromosomes 11 and 12, termed OsRINGC2-1 and OsRINGC2-2, respectively. A comparison of sequence divergences between 10 duplicate pairs on the distal parts of rice chromosomes 11 and 12 and randomly selected duplicate pairs suggested that OsRINGC2-1 and OsRINGC2-2 have evolved in concert via gene conversion. An in vitro ubiquitination assay revealed that both proteins possess E3 ligase activity, suggesting that the innate functions of these RING domains have not been affected by their modifications during evolution. Subcellular localizations were strikingly different; OsRINGC2-1 was found only in the cytoplasm with many punctate complexes, whereas OsRINGC2-2 was observed in both the nucleus and cytoplasm. The expression patterns of both genes showed striking differences in response to salt stress, whereas plants heterogeneous for both genes mediated salt tolerance in Arabidopsis, supporting the notion of concerted evolution. These results shed light on the molecular functions of OsRINGC2-1 and OsRINGC2-2 and provide insight into their molecular evolution.
Arabidopsis, Carrier Proteins, Chromosomes, Plant, Cytoplasm, Evolution, Molecular, Genes, Plant, Oryza, Plant Proteins, Protein Structure, Tertiary, Ubiquitin-Protein Ligases
null
22,705,027
2012-10-11
2012-07-24
1879-0038
Gene
Variable intron/exon structure in the oligochaete lombricine kinase gene.
Doumen Chris
eng
null
Journal Article, Research Support, Non-U.S. Gov't
Phosphotransferases (Nitrogenous Group Acceptor), lombricine kinase
IM
22705027, S0378-1119(12)00700-7, 10.1016/j.gene.2012.06.007
Lombricine kinase is an annelid enzyme that belongs to the phosphagen kinase family of which creatine kinase and arginine kinase are the typical representatives. The enzymes play important roles in the cellular energy metabolism of animals. Biochemical, physiological and molecular information with respect to lombricine kinase is limited compared to other phosphagen kinases. This study presents data on the cDNA sequences of lombricine kinase from two smaller oligochaetes, Enchytraeus sp. and Stylaria sp. The deduced amino acid sequences are analyzed and compared with other selected phosphagen kinases. The intron/exon structure of the lombricine kinase gene was determined for these two species as well as two additional oligochaetes, Lumbriculus variegatus and Tubifex tubifex, and compared with available data for annelid phosphagen kinases. The data indicate the existence of a variable organization of the proposed 8-intron/9-exon gene structure. The results provide further insights in the evolution and position of these enzymes within the phosphagen kinase family.
Amino Acid Sequence, Animals, Base Sequence, Evolution, Molecular, Exons, Genetic Variation, Introns, Molecular Sequence Data, Oligochaeta, Phosphotransferases (Nitrogenous Group Acceptor), Phylogeny, Sequence Alignment
null
22,705,028
2013-04-08
2022-03-10
1873-2933
Clinical biochemistry
Performance evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D assays.
Chen Yu, Kinney Lois, Božović Andrea, Smith Hilary, Tarr Heather, Diamandis Eleftherios P, LeBlanc Adrien
eng
Z99 CL999999 (Intramural NIH HHS, United States); ZIA CL010355-01 (Intramural NIH HHS, United States)
Comparative Study, Evaluation Study, Journal Article
Ergocalciferols, Cholecalciferol
IM
22705028, S0009-9120(12)00271-8, 10.1016/j.clinbiochem.2012.06.002, PMC4193681, NIHMS632834, 22247500, 22244986, 19563791, 20026023, 22238254, 18325178, 20601202, 21566493, 19109939, 22391026, 21348404, 20302938, 21646368, 21601563, 22411495, 17846391, 20795940, 19631201, 22334482, 22141317, 21646873, 18179991, 20142068, 21593503
To evaluate the newly developed Roche MODULAR Analytics E170 Total Vitamin D and the Siemens ADVIA Centaur Vitamin D Total assays.
Blood Chemical Analysis, Cholecalciferol, Ergocalciferols, Humans, Limit of Detection, Linear Models, Reference Standards, Signal-To-Noise Ratio, Tandem Mass Spectrometry
null
22,705,029
2013-03-12
2019-12-10
1768-3122
La Revue de medecine interne
[Crushing drugs in geriatric units: an "handicraft" practice with frequent errors which imposed recommendations].
Caussin M, Mourier W, Philippe S, Capet C, Adam M, Reynero N, Jouini C, Colombier A-S, Kadri K, Landrin I, Gréboval E, Rémy E, Marc F, Touflet M, Wirotius F, Delabre N, Le Hiress C, Rorteau V, Vimard M, Dufour M, Tharasse C, Dieu B, Varin R, Doucet J
fre
null
Evaluation Study, Journal Article
Capsules, Dosage Forms, Pharmaceutical Preparations
IM
22705029, S0248-8663(12)00530-9, 10.1016/j.revmed.2012.05.014
Swallowing disorders or psycho-behavioural distress frequently interfere on drug administration in elderly inpatients. Crushing drugs is a common although non validated practice. The objective of this first prospective study, performed in all geriatric units of the Rouen university hospital by a multidisciplinary group, was to assess the crushing practice, from the prescription to the administration of the drugs in order to elaborate corrective measures.
Administration, Oral, Aged, Aged, 80 and over, Capsules, Deglutition Disorders, Dosage Forms, Female, Geriatrics, Humans, Iatrogenic Disease, Incidence, Male, Medication Errors, Pharmaceutical Preparations, Practice Guidelines as Topic, Professional Practice
null
22,705,030
2013-05-17
2016-10-18
1768-3122
La Revue de medecine interne
[Epidemiology and management of isolated distal deep venous thrombosis].
Galanaud J-P, Kahn S R, Khau Van Kien A, Laroche J-P, Quéré I
fre
null
English Abstract, Journal Article, Review
Anticoagulants
IM
22705030, S0248-8663(12)00528-0, 10.1016/j.revmed.2012.05.012
Isolated distal deep-vein thromboses (DVT) are infra-popliteal DVT without involvement of proximal veins or pulmonary embolism (PE). They can affect deep calf (tibial anterior, tibial posterior, or peroneal) or muscular (gastrocnemius or soleal) veins. They represent half of all lower limbs DVT. Proximal and distal DVTs differ in terms of risk factor profile, proximal DVT being more frequently associated with chronic risk factors and distal DVT with transient ones. Their natural history (rate of spontaneous proximal extension) is debated leading to uncertainties on the need to diagnose and treat them with anticoagulant drugs. In the long term, the risk of venous thromboembolic recurrence is lower than that of proximal DVT and their absolute risk of post-thrombotic syndrome is unknown. French national guidelines suggest treating with anticoagulants for 6 weeks a first episode of isolated distal DVT provoked by a transient risk factor and treating for at least 3 months unprovoked or recurrent or active cancer-related distal DVT. The use of compression stockings use is suggested in case of deep calf vein thrombosis. Ongoing therapeutic trials should provide important data necessary to establish an evidence-based mode of care, especially about the need to treat distal DVT at low risk of extension with anticoagulants.
Anticoagulants, Humans, Leg, Lower Extremity, Models, Biological, Prevalence, Risk Factors, Venous Thrombosis
null