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22,705,534 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | Glycerine and levulinic acid: renewable co-substrates for the fermentative synthesis of short-chain poly(hydroxyalkanoate) biopolymers. | Ashby Richard D, Solaiman Daniel K Y, Strahan Gary D, Zhu Chengjun, Tappel Ryan C, Nomura Christopher T | eng | null | Journal Article | Biopolymers, Levulinic Acids, Polyhydroxyalkanoates, Glycerol, levulinic acid | IM | 22705534, S0960-8524(12)00845-0, 10.1016/j.biortech.2012.05.092 | Glycerine (a biodiesel co-product) and levulinic acid (a pulp and paper co-product) were used as co-substrates for the fermentative synthesis of short-chain polyhydroxyalkanoate (sc-PHA) biopolymers with tunable monomer and molecular weight characteristics. Pseudomonas oleovorans NRRL B-14682 utilized glycerine alone to produce poly(3-hydroxybutyrate) (PHB). When levulinic acid was added to the media at shake-flask scale in concentrations ≤0.6 wt.%, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V) copolymers were produced with 3-HV contents ranging from 37 to 97 mol%; a glycerine:levulinic acid ratio of 0.2%:0.8% (w/v) resulted in poly(3-hydroxyvalerate) (PHV). Ten-liter batch fermentations using glycerine:levulinic acid ratios of 1%:0, 0.75%:0.25%, 0.5%:0.5% and 0.25%:0.75% (w/v) resulted in PHB, P(73%-3HB-co-27%-3HV), P(30%-3HB-co-70%-3HV) and PHV with increasing number average molecular weights (×10(3) g/mol) of 328, 511, 728 and 1330, respectively, owing to glycerine-based chain termination. These results provide a novel means by which glycerine and levulinic acid can be used collectively to produce an array of distinct sc-PHA biopolymers. | Batch Cell Culture Techniques, Biomass, Biopolymers, Bioreactors, Calorimetry, Differential Scanning, Fermentation, Glycerol, Levulinic Acids, Molecular Weight, Polyhydroxyalkanoates, Pseudomonas, Recycling, Renewable Energy, Tensile Strength | null |
22,705,536 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Harvesting fresh water and marine algae by magnetic separation: screening of separation parameters and high gradient magnetic filtration. | Cerff Martin, Morweiser Michael, Dillschneider Robert, Michel Aymeé, Menzel Katharina, Posten Clemens | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705536, S0960-8524(12)00763-8, 10.1016/j.biortech.2012.05.020 | In this study, the focus is on magnetic separation of fresh water algae Chlamydomonas reinhardtii and Chlorella vulgaris as well as marine algae Phaeodactylum tricornutum and Nannochloropsis salina by means of silica-coated magnetic particles. Due to their small size and low biomass concentrations, harvesting algae by conventional methods is often inefficient and cost-consuming. Magnetic separation is a powerful tool to capture algae by adsorption to submicron-sized magnetic particles. Hereby, separation efficiency depends on parameters such as particle concentration, pH and medium composition. Separation efficiencies of >95% were obtained for all algae while maximum particle loads of 30 and 77 g/g were measured for C. reinhardtii and P. tricornutum at pH 8 and 12, respectively. This study highlights the potential of silica-coated magnetic particles for the removal of fresh water and marine algae by high gradient magnetic filtration and provides critical discussion on future improvements. | Adsorption, Aquatic Organisms, Eukaryota, Filtration, Fresh Water, Magnetics, Seawater, Static Electricity, Temperature | null |
22,705,535 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Effect of pretreatment by a microbial consortium on methane production of waste paper and cardboard. | Yuan Xufeng, Cao Yanzhuan, Li Jiajia, Wen Boting, Zhu Wanbin, Wang Xiaofen, Cui Zongjun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Polysaccharides, Volatile Organic Compounds, Waste Products, hemicellulose, Cellulose, Methane | IM | 22705535, S0960-8524(12)00802-4, 10.1016/j.biortech.2012.05.058 | A microbial consortium MC1 was used to pretreat filter paper, office paper, newspaper, and cardboard to enhance methane production. The results of pretreatment indicated that sCOD of hydrolysates of the four substrates increased significantly in the early stage, and peaked on day 7. During pretreatment, ethanol, acetic acid, propionic acid, butyric acid, and glycerol were the predominant volatile organic products in hydrolysates. MC1 had strong degradation ability on the four substrates, and the weight loss of filter paper, office paper, newspaper, and cardboard reached 78.3%, 80.5%, 39.7%, and 49.7%, respectively. The results of anaerobic digestion showed that methane production yields and rates of the four substrates significantly increased after pretreatment. This study is the first attempt to explore the microbial pretreatment method for anaerobic digestion of waste paper and cardboard. Microbial consortium pretreatment could be an effective method for enhancing methane production of waste paper and cardboard into bioenergy. | Anaerobiosis, Biological Oxygen Demand Analysis, Cellulose, Gas Chromatography-Mass Spectrometry, Hydrogen-Ion Concentration, Hydrolysis, Methane, Microbial Consortia, Paper, Polysaccharides, Volatile Organic Compounds, Waste Products | null |
22,705,537 | 2012-11-05 | 2017-11-16 | 1873-2976 | Bioresource technology | Composting versus vermicomposting: a comparative study of organic matter evolution through straight and combined processes. | Fornes Fernando, Mendoza-Hernández Daicy, García-de-la-Fuente Rosana, Abad Manuel, Belda Rosa M | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Organic Chemicals, Soil, Nitrogen | IM | 22705537, S0960-8524(12)00772-9, 10.1016/j.biortech.2012.05.028 | Changes in physical and chemical characteristics of an organic waste (tomato crop waste) throughout composting (COM), vermicomposting (VER) and the combination of both processes (C+V) were assessed at five selected stages. COM consisted of a combination of the static Rutgers system with forced aeration and pile turning. For VER Eisenia worms were fed with the raw material. For C+V pre-composted material was added to the worms. Particle size decreased during COM, yet it increased during VER and C+V due to the amalgamation of small particles. pH was alkaline throughout the processes. Heavy irrigation during vermicomposting resulted in greater decrease of EC and greater leaching of organic matter, total nitrogen and most macronutrients in VER and C+V than in COM. Final materials were not phytotoxic but compost could have salinity related problems. Thus, COM, VER and C+V produced treated organic material, which could be suitable for horticultural purposes. | Animals, Biodegradation, Environmental, Chemical Phenomena, Nitrogen, Oligochaeta, Organic Chemicals, Refuse Disposal, Soil | null |
22,705,538 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Combination of pyrolysis and hydroliquefaction of CCB-treated wood for energy recovery: optimization and products characterization. | Kinata Silao Espérance, Loubar Khaled, Paraschiv Maria, Belloncle Christophe, Tazerout Mohand | eng | null | Journal Article | Elements, Gases, Solvents, Water, Chromium, Charcoal, Copper, Hydrogen, Boron | IM | 22705538, S0960-8524(12)00760-2, 10.1016/j.biortech.2012.05.017 | In this paper, pyrolysis and hydroliquefaction processes were successively used to convert CCB-treated wood into bio-oil with respect to environment. Pyrolysis temperature has been optimized to produce maximum yield of charcoal with a high metal content (Cu, Cr, and B). The results obtained indicate that the pyrolysis at 300 °C and 30 min are the optimal conditions giving high yield of charcoal about 45% which contains up to 94% of Cu, 100% of Cr and 88% of B. After pyrolysis process, the charcoal has been converted into bio-oil using hydroliquefaction process. The optimization approach for the yield of bio-oil using a complete factorial design with three parameters: charcoal/solvent, temperature and hydrogen pressure was discussed. It is observed that the temperature is the most significant parameter and the optimum yield of bio-oil is around 82%. The metal analysis shows that the metals present in the bio-oil is very negligible. | Biotechnology, Boron, Charcoal, Chromium, Copper, Elements, Energy-Generating Resources, Gases, Hydrogen, Pressure, Recycling, Solvents, Temperature, Water, Wood | null |
22,705,539 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Numerical analysis of the impact of structural changes in cellulosic substrates on enzymatic saccharification. | Seo Dong-June, Fujita Hirotaka, Sakoda Akiyoshi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cellulose, Cellulase | IM | 22705539, S0960-8524(12)00783-3, 10.1016/j.biortech.2012.05.039 | Here, a simple cellulose conversion model that considers the cellulose surface area and surface density of adsorbed cellulase as substrate-derived and cellulase-derived factors controlling reaction rates is provided. Microcrystalline cellulose (Avicel) and delignifed softwood were used as controls, and structure-modified samples were prepared. It was shown that the initial cellulose conversion rate is largely controlled by the cellulose surface area. Moreover, the proposed model demonstrates that increases in cellulose surface area reduce retardation of the cellulase reaction. The proposed model was used to estimate the impact of structural changes in a substrate (i.e., cellulose surface area) by pre-treatment on enzymatic saccharification. It was found that increasing the cellulose surface area is the most effective way to optimize enzymatic saccharification of cellulose substrates. | Adsorption, Biotechnology, Carbohydrate Metabolism, Cellulase, Cellulose, Models, Biological, Numerical Analysis, Computer-Assisted, Reproducibility of Results, Substrate Specificity, Temperature, Time Factors | null |
22,705,542 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | Catalytic etherification of glycerol to produce biofuels over novel spherical silica supported Hyflon® catalysts. | Frusteri Francesco, Frusteri Leone, Cannilla Catia, Bonura Giuseppe | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Alkenes, Biofuels, Ethers, Gasoline, Silicon Dioxide, Glycerol, isobutylene | IM | 22705542, S0960-8524(12)00727-4, 10.1016/j.biortech.2012.04.103 | Etherification of glycerol (GLY) with isobutylene (IB) to produce biofuels was investigated in liquid phase using spherical silica supported Hyflon® catalysts (SSHC). As reference catalyst, Amberlyst® 15 (A-15) acid ion-exchange resin was used. Experiments were carried out in batch mode at a reaction temperature ranging from 323 to 343 K. SSHC were found to be very effective systems in etherification of glycerol with IB, providing cumulative di- and tri-ethers yields higher than that obtained by using A-15 catalyst. Furthermore, such catalysts were stable and easily reusable; no leaching of active phase was observed. The formation of poly-substituted ethers, suitable additives for conventional fuels, was favored by operating at an isobutylene/glycerol molar ratio >3 and low reaction time (<6 h); however, the concentration of mono-ether reached values lower than 3 wt.% only when SSHC catalyst was used. Turnover frequency of glycerol (TOF(GLY)) highlighted that SSHC systems were much more active than A-15 catalyst: the accessibility and nature of active sites and the surface properties of catalysts were indicated as the main factors affecting the catalytic behavior. A lower acid site density of SSHC than that of A-15 catalyst was decisive in preventing the occurrence of oligomerization reaction which leads to the formation of di-isobutylene (DIB), precursors of gummy products. | Alkenes, Biofuels, Catalysis, Chemical Phenomena, Ethers, Gasoline, Glycerol, Porosity, Recycling, Reference Standards, Silicon Dioxide, Structure-Activity Relationship, Temperature, Time Factors | null |
22,705,540 | 2012-11-05 | 2022-01-29 | 1873-2976 | Bioresource technology | Mineral concentrations in solid fuels from European semi-natural grasslands after hydrothermal conditioning and subsequent mechanical dehydration. | Hensgen Frank, Bühle Lutz, Donnison Iain, Frasier Mariecia, Vale Jim, Corton John, Heinsoo Katrin, Melts Indrek, Wachendorf Michael | eng | BBS/E/W/00003134E (Biotechnology and Biological Sciences Research Council, United Kingdom) | Journal Article, Research Support, Non-U.S. Gov't | Biofuels, Elements, Minerals, Soil, Water, Magnesium, Nitrogen | IM | 22705540, S0960-8524(12)00779-1, 10.1016/j.biortech.2012.05.035 | The integrated generation of solid fuel and biogas from biomass (IFBB) is particularly designed for the conversion of semi-natural and high biodiversity grassland biomass into energy. This biomass is problematic in common energy conversion techniques, e.g. biogas conversion or combustion, because of its chemical composition. The IFFB process separates the material into a fibre rich solid fuel and a fluid, which is rich in minerals and highly digestible constituents and is used for anaerobic digestion. Biomasses from 18 European semi-natural grassland sites have been processed in an IFBB prototype. The impact of different chemical and botanical parameters on mass flow of mineral plant compounds and their concentrations in the fuel has been investigated. Fuel quality was significantly influenced by chemical and botanical parameters and the quality could be significantly improved during processing. Biomass with a high grass proportion and fibre content showed the best fuel qualities after IFBB treatment. | Biofuels, Biomass, Desiccation, Ecosystem, Elements, Europe, Magnesium, Mechanical Phenomena, Minerals, Nitrogen, Poaceae, Rheology, Silage, Soil, Temperature, Water | null |
22,705,541 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Comparison of mixed-acid fermentations inoculated with six different mixed cultures. | Forrest Andrea K, Hollister Emily B, Gentry Terry J, Wilkinson Heather H, Holtzapple Mark T | eng | null | Comparative Study, Journal Article | Carboxylic Acids | IM | 22705541, S0960-8524(12)00787-0, 10.1016/j.biortech.2012.05.043 | The MixAlco™ process biologically converts biomass to carboxylate salts that may be converted to a variety of chemicals and fuels. This study examines the fermentation performance of six different mixed cultures, and how the performance was affected by the bacterial composition of each community. All six countercurrent fermentations had very similar performance, but were dissimilar in microbial community composition. The acid concentrations varied by only 12% between fermentation trains and the conversions varied only by 6%. The microbial communities were profiled using 16S rRNA tag-pyrosequencing, which revealed the presence of dynamic communities that were dominated by bacteria resembling Clostridia, but they shared few taxa in common. Yue-Clayton similarity calculations of the communities revealed that they were extremely different. The presence of different but functionally similar microbial communities in this study suggests that it is the operating parameters that determine the fermentation end-products. | Bacteria, Bioreactors, Carboxylic Acids, Countercurrent Distribution, Fermentation, Soil Microbiology | null |
22,705,543 | 2012-11-19 | 2012-07-17 | 1096-0325 | Theoretical population biology | Coexistence and community structure in a consumer resource model with implicit stoichiometry. | Orlando Paul A, Brown Joel S, Wise David H | eng | null | Journal Article | null | IM | 22705543, S0040-5809(12)00061-5, 10.1016/j.tpb.2012.05.005 | We combine stoichiometry theory and optimal foraging theory into the MacArthur consumer-resource model. This generates predictions for diet choice, coexistence, and community structure of heterotroph communities. Tradeoffs in consumer resource-garnering traits influence community outcomes. With scarce resources, consumers forage opportunistically for complementary resources and may coexist via tradeoffs in resource encounter rates. In contrast to single currency models, stoichiometry permits multiple equilibria. These alternative stable states occur when tradeoffs in resource encounter rates are stronger than tradeoffs in elemental conversion efficiencies. With abundant resources consumers exhibit partially selective diets for essential resources and may coexist via tradeoffs in elemental conversion efficiencies. These results differ from single currency models, where adaptive diet selection is either opportunistic or selective. Interestingly, communities composed of efficient consumers share many of the same properties as communities based on substitutable resources. However, communities composed of relatively inefficient consumers behave similarly to plant communities as characterized by Tilman's consumer resource theory. The results of our model indicate that the effects of stoichiometry theory on community ecology are dependent upon both consumer foraging behavior and the nature of resource garnering tradeoffs. | Competitive Behavior, Ecosystem, Feeding Behavior, Models, Theoretical, Population Dynamics | null |
22,705,544 | 2012-12-03 | 2018-12-01 | 1421-976X | Caries research | 59th ORCA Congress. June 27-30, 2012, Cabo Frio, Brazil. | null | eng | null | Congress, Overall | null | IM | 22705544, 000338545, 10.1159/000338545 | null | Dental Caries, Humans, Research | null |
22,705,545 | 2013-05-01 | 2023-11-20 | 1872-8294 | Advanced drug delivery reviews | On the roles of polyvalent binding in immune recognition: perspectives in the nanoscience of immunology and the immune response to nanomedicines. | Vorup-Jensen Thomas | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Cell Adhesion Molecules, Receptors, Cell Surface | IM | 22705545, S0169-409X(12)00200-1, 10.1016/j.addr.2012.06.003 | Immunology often conveys the image of large molecules, either in the soluble state or in the membrane of leukocytes, forming multiple contacts with a target for actions of the immune system. Avidity names the ability of a polyvalent molecule to form multiple connections of the same kind with ligands tethered to the same surface. Polyvalent interactions are vastly stronger than their monovalent equivalent. In the present review, the functional consequences of polyvalent interactions are explored in a perspective of recent theoretical advances in understanding the thermodynamics of such binding. From insights on the structural biology of soluble pattern recognition molecules as well as adhesion molecules in the cell membranes or in their proteolytically shed form, this review documents the prominent role of polyvalent interactions in making the immune system a formidable barrier to microbial infection as well as constituting a significant challenge to the application of nanomedicines. | Animals, Cell Adhesion Molecules, Cell Membrane, Humans, Immune System, Leukocytes, Nanomedicine, Nanoparticles, Receptors, Cell Surface, Thermodynamics | null |
22,705,546 | 2013-09-27 | 2013-05-06 | 1872-8294 | Advanced drug delivery reviews | Controlled delivery for neuro-bionic devices. | Yue Zhilian, Moulton Simon E, Cook Mark, O'Leary Stephen, Wallace Gordon G | eng | null | Journal Article, Review | Polymers | IM | 22705546, S0169-409X(12)00199-8, 10.1016/j.addr.2012.06.002 | Implantable electrodes interface with the human body for a range of therapeutic as well as diagnostic applications. Here we provide an overview of controlled delivery strategies used in neuro-bionics. Controlled delivery of bioactive molecules has been used to minimise reactive cellular and tissue responses and/or promote nerve preservation and neurite outgrowth toward the implanted electrode. These effects are integral to establishing a chronically stable and effective electrode-neural communication. Drug-eluting bioactive coatings, organic conductive polymers, or integrated microfabricated drug delivery channels are strategies commonly used. | Bioengineering, Drug Delivery Systems, Electrodes, Implanted, Humans, Polymers | null |
22,705,547 | 2013-05-01 | 2012-11-19 | 1872-8294 | Advanced drug delivery reviews | The influence of nanostructured materials on biointerfacial interactions. | Koegler Peter, Clayton Andrew, Thissen Helmut, Santos Gil Nonato C, Kingshott Peter | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Biocompatible Materials, Proteins | IM | 22705547, S0169-409X(12)00198-6, 10.1016/j.addr.2012.06.001 | Control over biointerfacial interactions in vitro and in vivo is the key to many biomedical applications: from cell culture and diagnostic tools to drug delivery, biomaterials and regenerative medicine. The increasing use of nanostructured materials is placing a greater demand on improving our understanding of how these new materials influence biointerfacial interactions, including protein adsorption and subsequent cellular responses. A range of nanoscale material properties influence these interactions, and material toxicity. The ability to manipulate both material nanochemistry and nanotopography remains challenging in its own right, however, a more in-depth knowledge of the subsequent biological responses to these new materials must occur simultaneously if they are ever to be affective in the clinic. We highlight some of the key technologies used for fabrication of nanostructured materials, examine how nanostructured materials influence the behavior of proteins and cells at surfaces and provide details of important analytical techniques used in this context. | Adsorption, Animals, Biocompatible Materials, Cell Culture Techniques, Drug Delivery Systems, Humans, Nanostructures, Nanotechnology, Proteins, Regenerative Medicine | null |
22,705,548 | 2012-10-22 | 2012-07-17 | 1090-2104 | Biochemical and biophysical research communications | Inhibition of erythropoiesis by Smad6 in human cord blood hematopoietic stem cells. | Kang Young-Ju, Shin Ji-woong, Yoon Jeong-Hwan, Oh Il-Hwan, Lee Soon-Pyo, Kim Suk-Young, Park Seok Hee, Mamura Mizuko | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antigens, CD34, BMP2 protein, human, BMP4 protein, human, BMP6 protein, human, BMP7 protein, human, Bone Morphogenetic Protein 2, Bone Morphogenetic Protein 4, Bone Morphogenetic Protein 6, Bone Morphogenetic Protein 7, GATA2 Transcription Factor, GATA2 protein, human, Kruppel-Like Transcription Factors, SMAD6 protein, human, Smad6 Protein, erythroid Kruppel-like factor, Bone Morphogenetic Protein Receptors, Type I | IM | 22705548, S0006-291X(12)01119-9, 10.1016/j.bbrc.2012.06.031 | Bone morphogenetic proteins (BMPs) that belong to the transforming growth factor-β (TGF-β) superfamily cytokines, play crucial roles in hematopoiesis. However, roles of Smad6 in hematopoiesis remained unknown in contrast to the other inhibitory Smad (I-Smad), Smad7. Here we show that Smad6 inhibits erythropoiesis in human CD34(+) cord blood hematopoietic stem cells (HSCs). Smad6 was specifically expressed in CD34(+) cord blood HSCs, which was correlated with the expression of BMP2/4/6/7 and BMP type I receptor (BMPRI). BMP-specific receptor-regulated Smads (R-Smads), Smad1 and Smad5 in cooperation with Smad4 induced transcription of the Smad6 gene. Instead of affecting cell cycle, apoptosis, self-renewal, and stemness of CD34(+) cells, Smad6 knockdown enhanced, whereas Smad6 overexpression suppressed erythropoiesis in stem cell culture and colony formation assay. Consistently, Smad6 suppressed the expression of the genes essential for erythropoiesis, such as Kruppel-like factor 1 (erythroid) (KLF1/EKLF) and GATA binding protein 2 (GATA-2). Promoter analyses showed that Smad6 repressed Smad5/4-induced transcription of the Klf1 gene. Thus, our data suggest that Smad6 indirectly maintains stemness by preventing spontaneous erythropoiesis in HSCs. | Antigens, CD34, Bone Morphogenetic Protein 2, Bone Morphogenetic Protein 4, Bone Morphogenetic Protein 6, Bone Morphogenetic Protein 7, Bone Morphogenetic Protein Receptors, Type I, Cells, Cultured, Erythropoiesis, Fetal Blood, GATA2 Transcription Factor, Gene Expression Regulation, Gene Knockdown Techniques, Hematopoietic Stem Cells, Humans, Kruppel-Like Transcription Factors, Promoter Regions, Genetic, Smad6 Protein, Transcription, Genetic | null |
22,705,550 | 2012-10-22 | 2012-07-17 | 1090-2104 | Biochemical and biophysical research communications | Differential polyubiquitin recognition by tandem ubiquitin binding domains of Rabex-5. | Shin Donghyuk, Lee Sei Young, Han Seungsoo, Ren Shuo, Kim Soyoun, Aikawa Yoshikatsu, Lee Sangho | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Guanine Nucleotide Exchange Factors, Polyubiquitin | IM | 22705550, S0006-291X(12)01120-5, 10.1016/j.bbrc.2012.06.032 | Linkage-specific polyubiquitination regulates many cellular processes. The N-terminal fragment of Rabex-5 (Rabex-5(9-73)) contains tandem ubiquitin binding domains: A20_ZF and MIU. The A20_ZF-MIU of Rabex-5 is known to bind monoubiquitin but molecular details of polyubiquitin binding affinity and linkage selectivity by Rabex-5(9-73) remain elusive. Here we report that Rabex-5(9-73) binds linear, K63- and K48-linked tetraubiquitin (Ub(4)) chains with K(d) of 0.1-1 μM, determined by biolayer interferometry. Mutational analysis of qualitative and quantitative binding data reveals that MIU is more important than A20_ZF in linkage-specific polyubiquitin recognition. MIU prefers binding to linear and K63-linked Ub(4) with sub μM affinities. However, A20_ZF recognizes the three linkage-specific Ub(4) with similar affinities with K(d) of 3-4 μM, unlike ZnF4 of A20. Taken together, our data suggest differential physiological roles of the two ubiquitin binding domains in Rabex-5. | Amino Acid Sequence, Animals, Cattle, Guanine Nucleotide Exchange Factors, Humans, Molecular Sequence Data, Polyubiquitin, Protein Structure, Tertiary | null |
22,705,549 | 2012-10-22 | 2021-10-21 | 1090-2104 | Biochemical and biophysical research communications | Hydrogen peroxide induces stress granule formation independent of eIF2α phosphorylation. | Emara Mohamed M, Fujimura Ken, Sciaranghella Daniele, Ivanova Victoria, Ivanov Pavel, Anderson Paul | eng | R56 AI033600 (NIAID NIH HHS, United States); AI065858 (NIAID NIH HHS, United States); P01 AI065858 (NIAID NIH HHS, United States); R01 AI033600 (NIAID NIH HHS, United States); AI033600 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Eukaryotic Initiation Factor-2, RNA, Small Interfering, RNA, Untranslated, Hydrogen Peroxide | IM | 22705549, S0006-291X(12)01121-7, 10.1016/j.bbrc.2012.06.033, PMC3399031, NIHMS387344, 10613902, 21126188, 18794846, 17030858, 20142804, 16532013, 20156963, 18417611, 12613655, 16849329, 11121440, 18356035, 19823042, 18034160, 21172806, 11691836, 21979918, 20129916, 17908917, 19467203, 16337595, 9712873, 22012617, 15743837, 19239892, 21855800, 11809833, 18836437, 16951406, 12071973, 11430820, 21911475, 18291657, 16520386, 17923231, 19332886, 19111193, 19333389 | In cells exposed to environmental stress, inhibition of translation initiation conserves energy for the repair of cellular damage. Untranslated mRNAs that accumulate in these cells move to discrete cytoplasmic foci known as stress granules (SGs). The assembly of SGs helps cells to survive under adverse environmental conditions. We have analyzed the mechanism by which hydrogen peroxide (H(2)O(2))-induced oxidative stress inhibits translation initiation and induces SG assembly in mammalian cells. Our data indicate that H(2)O(2) inhibits translation and induces the assembly of SGs. The assembly of H(2)O(2)-induced SGs is independent of the phosphorylation of eIF2α, a major trigger of SG assembly, but requires remodeling of the cap-binding eIF4F complex. Moreover, H(2)O(2)-induced SGs are compositionally distinct from canonical SGs, and targeted knockdown of eIF4E, a protein required for canonical translation initiation, inhibits H(2)O(2)-induced SG assembly. Our data reveal new aspects of translational regulation induced by oxidative insults. | Animals, Cell Line, Tumor, Cytoplasmic Granules, Eukaryotic Initiation Factor-2, Gene Knockdown Techniques, Humans, Hydrogen Peroxide, Mice, Oxidative Stress, Phosphorylation, Protein Biosynthesis, RNA, Small Interfering, RNA, Untranslated | null |
22,705,551 | 2012-11-20 | 2013-11-21 | 1530-0315 | Medicine and science in sports and exercise | Predictors of three-dimensional breast kinematics during bare-breasted running. | Wood Louise Ellen, White Jennifer, Milligan Alexandra, Ayres Bessie, Hedger Wendy, Scurr Joanna | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705551, 10.1249/MSS.0b013e31824bd62c, 00005768-201207000-00020 | This study aimed to analyze differences in breast kinematics between breast cup sizes during running and the ability of breast and body size measurements to explain these differences. | Adult, Biomechanical Phenomena, Body Mass Index, Breast, Exercise Test, Female, Humans, Organ Size, Running, Young Adult | null |
22,705,552 | 2012-11-20 | 2018-12-01 | 1530-0315 | Medicine and science in sports and exercise | A critical examination of the Douglas bag technique. | Shephard Roy J | eng | null | Letter, Comment | null | IM | 22705552, 10.1249/MSS.0b013e318253b1c3, 00005768-201207000-00028 | null | Exercise Test, Humans, Male, Spirometry | null |
22,705,555 | 2012-12-11 | 2012-09-14 | 1879-0089 | Developmental and comparative immunology | Diversification of IFNγ-inducible CXCb chemokines in cyprinid fish. | van der Aa Lieke M, Chadzinska Magdalena, Derks Wouter, Scheer Marleen, Levraud Jean-Pierre, Boudinot Pierre, Lidy Verburg-van Kemenade B M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Chemokines, CXC, Lipopolysaccharides, Interferon-gamma, Zymosan | IM | 22705555, S0145-305X(12)00112-7, 10.1016/j.dci.2012.05.005 | We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-γ-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-γ2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9-11. | Animals, Carps, Chemokines, CXC, Cloning, Molecular, Evolution, Molecular, Interferon-gamma, Leukocytes, Lipopolysaccharides, Peritoneum, Peritonitis, Promoter Regions, Genetic, Transcriptome, Zebrafish, Zymosan | null |
22,705,556 | 2012-11-26 | 2013-11-21 | 1531-4332 | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology | Myosin heavy chain and parvalbumin expression in swimming and feeding muscles of centrarchid fishes: the molecular basis of the scaling of contractile properties. | Campion L A, Choi S, Mistry H L, Coughlin D J | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fish Proteins, Parvalbumins, Protein Isoforms, Succinate Dehydrogenase, Myosin Heavy Chains | IM | 22705556, S1095-6433(12)00345-5, 10.1016/j.cbpa.2012.06.001 | In centrarchid fishes, such as bluegill (Lepomis macrochirus, Rafinesque) and largemouth bass (Micropterus salmoides, Lacepède), the contractile properties of feeding and swimming muscles show different scaling patterns. While the maximum shortening velocity (V(max)) and rate of relaxation from tetanus of swimming or myotomal muscle slow with growth, the feeding muscle shows distinctive scaling patterns. Cranial epaxial muscle, which is used to elevate the head during feeding strikes, retains fast contractile properties across a range of fish sizes in both species. In bass, the sternohyoideous muscle, which depresses the floor of the mouth during feeding strikes, shows faster contractile properties with growth. The objective of this study was to determine the molecular basis of these different scaling patterns. We examined the expression of two muscle proteins, myosin heavy chain (MyHC) and parvalbumin (PV), that affect contractile properties. We hypothesized that the relative contribution of slow and fast MyHC isoforms will modulate V(max) in these fishes, while the presence of PV in muscle will enhance rates of muscle relaxation. Myotomal muscle displays an increase in sMyHC expression with growth, in agreement with its physiological properties. Feeding muscles such as epaxial and sternohyoideus show no change or a decrease in sMyHC expression with growth, again as predicted from contractile properties. PV expression in myotomal muscle decreases with growth in both species, as has been seen in other fishes. The feeding muscles again show no change or an increase in PV expression with growth, contributing to faster contractile properties in these fishes. Both MyHC and PV appear to play important roles in modulating muscle contractile properties of swimming and feeding muscles in centrarchid fishes. | Animals, Fish Proteins, Mitochondria, Muscle, Muscle Contraction, Muscle Development, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myosin Heavy Chains, Parvalbumins, Perciformes, Protein Isoforms, Succinate Dehydrogenase, Swimming | null |
22,705,557 | 2012-11-26 | 2014-11-20 | 1531-4332 | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology | Morpho-functional characterization of the goldfish (Carassius auratus L.) heart. | Garofalo F, Imbrogno S, Tota B, Amelio D | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fish Proteins, Nitric Oxide Synthase Type III | IM | 22705557, S1095-6433(12)00346-7, 10.1016/j.cbpa.2012.05.206 | Using morphological and physiological approaches we provided, for the first time, a structural and functional characterization of Carassius auratus L. heart. Besides to the classical four chambers, i.e. sinus venosus, atrium, ventricle, bulbus, we described two distinct structures corresponding to the atrio-ventricular (AV) region and the conus arteriosus. The atrium is very large and highly trabeculated; the ventricle shows an outer compacta, vascularized by coronary vessels, and an inner spongiosa; the bulbus wall is characterized by a high elastin/collagen ratio, which makes it extremely compliant. Immunolocalization revealed a strong expression of activated "eNOS-like" isoforms both at coronary endothelium and, to a lesser extent, in the myocardiocytes and the endocardial endothelium (EE). The structural design of the heart appears to comply with its mechanical function. Using an in vitro working heart preparation, cardiac performance was evaluated at different filling and afterload pressures. The hearts were very sensitive to filling pressure increases. Maximum Stroke volume (SV=1.08 ± 0.09 mL/kg body mass) was obtained with an input pressure of 0.4 kPa. The heart was not able to sustain afterload increases, values higher than 1.5 kPa impairing its performance. These morpho-functional features are consistent with a volume pump mechanical performance. | Animals, Coronary Vessels, Fish Proteins, Goldfish, Heart, Heart Rate, In Vitro Techniques, Myocardium, Nitric Oxide Synthase Type III, Stroke Volume | null |
22,705,558 | 2012-10-26 | 2013-11-21 | 1361-6528 | Nanotechnology | Application of in situ measurement of photo-induced variations in electron work function for in-depth understanding of the photocatalytic activity of TiO2 nanotubes. | Fu Ning, Tang Xinhu, Li Dongyang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | titanium dioxide, Titanium | IM | 22705558, 10.1088/0957-4484/23/27/275704 | The electron work function (EWF) is the minimum energy required to move an electron at the Fermi level from inside a conducting material to its surface with zero kinetic energy. This fundamental parameter is directly related to many chemical, physical, and mechanical properties of materials. In this work, variations in EWF of TiO(2) nanotube arrays under light illumination were monitored in situ using a Kelvin probe in order to study the photon-induced electron excitation in the TiO(2) nanotubular arrays upon illumination. It was shown that the EWF could be used to investigate the electron-hole separation and recombination, helping us to better understand the photo-activity of the photocatalytic material. This study has demonstrated that EWF provides an effective parameter for understanding of semiconductors' photo-activities with different views that may not be achieved using traditional techniques, such as diffuse reflection spectroscopy and photoelectrochemical measurement. | Catalysis, Electron Transport, Light, Materials Testing, Nanotubes, Particle Size, Titanium | null |
22,705,559 | 2012-12-04 | 2012-07-03 | 1364-5528 | The Analyst | Attenuated total reflection infrared microspectroscopy combined with multivariate analysis: a novel tool to study the presence of cocoa polyphenol metabolites in urine samples. | Sánchez-González Claudia, Nigussie Worku, Estruch Ramon, Lamuela-Raventós Rosa M, Izquierdo-Pulido Maria, de Lamo-Castellví Silvia | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Polyphenols | IM | 22705559, 10.1039/c2an35286a | The detection and quantification of polyphenols in biological samples is mainly performed by liquid chromatography in tandem with mass spectrometry (HPLC-MS/MS). This technique requires the use of organic solvents and needs control and maintenance of several MS/MS parameters, which makes the method expensive and time consuming. The main objective of this study was to evaluate, for the first time, the potential of using attenuated total reflection infrared microspectroscopy (ATR-IRMS) coupled with multivariate analysis to detect and quantify phenolic compounds excreted in human urine. Samples were collected from 5 healthy volunteers before and 6, 12 and 24 h after ingestion of 40 g cocoa powder with 250 mL of water or whole milk, and stored at -80 °C. Each sample was centrifuged at 5000 rpm for 10 min and at 4 °C and applied onto grids of a hydrophobic membrane. Spectra were collected in the attenuated total reflection (ATR) mode in the mid-infrared region (4000-800 cm(-1)) and were analyzed by a multivariate analysis technique, soft independent modeling of class analogy (SIMCA). Spectral models showed that IR bands responsible for chemical differences among samples were related to aromatic rings. Therefore, ATR-IRMS could be an interesting and straightforward technique for the detection of phenolic compounds excreted in urine. Moreover, it could be a valuable tool in studies aimed to identify biomarkers of consumption of polyphenol-rich diets. | Cacao, Humans, Multivariate Analysis, Polyphenols, Reference Values, Spectroscopy, Fourier Transform Infrared | null |
22,705,560 | 2013-01-09 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | MetaLocGramN: A meta-predictor of protein subcellular localization for Gram-negative bacteria. | Magnus Marcin, Pawlowski Marcin, Bujnicki Janusz M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Bacterial Proteins, Protein Sorting Signals | IM | 22705560, S1570-9639(12)00118-5, 10.1016/j.bbapap.2012.05.018 | Subcellular localization is a key functional characteristic of proteins. It is determined by signals encoded in the protein sequence. The experimental determination of subcellular localization is laborious. Thus, a number of computational methods have been developed to predict the protein location from sequence. However predictions made by different methods often disagree with each other and it is not always clear which algorithm performs best for the given cellular compartment. We benchmarked primary subcellular localization predictors for proteins from Gram-negative bacteria, PSORTb3, PSLpred, CELLO, and SOSUI-GramN, on a common dataset that included 1056 proteins. We found that PSORTb3 performs best on the average, but is outperformed by other methods in predictions of extracellular proteins. This motivated us to develop a meta-predictor, which combines the primary methods by using the logistic regression models, to take advantage of their combined strengths, and to eliminate their individual weaknesses. MetaLocGramN runs the primary methods, and based on their output classifies protein sequences into one of five major localizations of the Gram-negative bacterial cell: cytoplasm, plasma membrane, periplasm, outer membrane, and extracellular space. MetaLocGramN achieves the average Matthews correlation coefficient of 0.806, i.e. 12% better than the best individual primary method. MetaLocGramN is a meta-predictor specialized in predicting subcellular localization for proteins from Gram-negative bacteria. According to our benchmark, it performs better than all other tools run independently. MetaLocGramN is a web and SOAP server available for free use by all academic users at the URL http://iimcb.genesilico.pl/MetaLocGramN. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction. | Bacterial Proteins, Databases, Protein, Gram-Negative Bacteria, Internet, Protein Sorting Signals, Sequence Analysis, Protein, Subcellular Fractions | null |
22,705,561 | 2013-03-08 | 2022-12-07 | 1879-0720 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | Post-expansile hydrogel foam aerosol of PG-liposomes: a novel delivery system for vaginal drug delivery applications. | Li Wei-Ze, Zhao Ning, Zhou Yong-Qiang, Yang Li-Bin, Xiao-Ning Wang, Bao-Hua Hao, Peng Kong, Chun-Feng Zhang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Aerosols, Alkaloids, Liposomes, Quinolizines, Vaginal Creams, Foams, and Jellies, Hydrogel, Polyethylene Glycol Dimethacrylate, Propylene Glycol, Matrines | IM | 22705561, S0928-0987(12)00245-X, 10.1016/j.ejps.2012.06.001 | The purpose of this work was to develop and characterize a novel delivery system of post-expansile hydrogel foam aerosol of propylene glycol-embodying liposomes (PG-liposomes) (PEHFL) for vaginal drug delivery applications. Matrine (MT) was used as a model drug to investigate the vaginal mucous membrane permeation behavior of MT from PEHFL versus PG-liposomes foam aerosol (PLFA), hydrogel foam aerosol (HFA) and hydrogel (HYG). The MT loaded PG-liposomes were characterized for shape, particle size, polydispersity, zeta potential and encapsulation efficiency; the foams of PEHFL were also characterized for swelling behavior, mucoadhesive force and duration. Results revealed that: (i) the MT loaded PG-liposomes had a mean size of 122 ± 16 nm, a good polydispersity index of 0.147 ± 0.023, and exhibited a negative charge of -47.5 ± 0.4 mV, the MT entrapment capacity in PG-liposomes (was calculated as percentages of total drug) was 80.8 ± 2.6%; (ii) the foams of PEHFL had a laggingly swelling process after spurted from a sealed container, and the higher the temperature of the surrounding environment, the greater the degree of swelling, this swelling state of foams would greatly enhance drug spread uniformly in vaginal canal and contact the vaginal walls tightly; (iii) the foams of PEHFL had a mucoadhesive force about 1460 ± 123 mN/cm(2), and could sustain 85 ± 11 min in vitro; (iv) the overall mean permeated MT through unit mass of porcine vaginal tissue from PEHFL was 2.64, 2.34 and 7.59 times higher than that from PLFA, HFA and HYG, respectively (t-test, P<0.05); and the quantity of MT remaining in the vaginal tissue at the end of the 12h experiment was also significantly greater (t-test, P<0.05) from the PEHFL than from PLFA, HFA and HYG. All of these results indicate that the main advantages of PEHFL over conventional dosage forms are the ability to enhance the vaginal mucosa permeability of MT, spread uniformly in vaginal canal especially the highly folded epithelial surfaces, prolong the residence time at the site of administration and induce MT delayed release. In conclusion, the PEHFL may be a promising delivery system for vaginal delivery of medication. | Aerosols, Alkaloids, Animals, Drug Delivery Systems, Drug Stability, Female, Humans, Hydrogel, Polyethylene Glycol Dimethacrylate, Liposomes, Mucous Membrane, Particle Size, Permeability, Propylene Glycol, Quinolizines, Swine, Vagina, Vaginal Creams, Foams, and Jellies, Matrines | null |
22,705,564 | 2013-01-22 | 2021-10-21 | 1745-7262 | Asian journal of andrology | Pumilio 1 control of spermatogenesis: a roadmap for future research. | Huszar Gabor | eng | null | Comment, Journal Article | null | null | 22705564, aja201238, 10.1038/aja.2012.38, PMC3735002, 15044602, 17509211, 17572691, 22342750 | null | null | null |
22,705,563 | 2013-01-22 | 2022-04-08 | 1745-7262 | Asian journal of andrology | Vascular endothelial growth factor A, secreted in response to transforming growth factor-β1 under hypoxic conditions, induces autocrine effects on migration of prostate cancer cells. | Darrington Eric, Zhong Miao, Vo Bao-Han, Khan Shafiq A | eng | G12 RR003062 (NCRR NIH HHS, United States); P20 MD002285 (NIMHD NIH HHS, United States); 1P20MD002285 (NIMHD NIH HHS, United States); 5G12RR003062 (NCRR NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | DNA Primers, RNA, Messenger, Transforming Growth Factor beta1, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1 | IM | 22705563, aja2011197, 10.1038/aja.2011.197, PMC3476842, NIHMS394657, 18849330, 12171905, 21057546, 16691538, 11421617, 20065063, 20663860, 16904831, 16267000, 10475365, 9720590, 12885781, 19363294, 7833842, 9529250, 20141840, 7474936, 19727403, 15743179, 19821979, 7479819, 20385358, 15035619, 11485923, 12778165, 20085644, 12065755, 1417831, 1312256, 19171888, 9223322, 9188862, 11486006 | Hypoxia and transforming growth factor-β1 (TGF-β1) increase vascular endothelial growth factor A (VEGFA) expression in a number of malignancies. This effect of hypoxia and TGF-β1 might be responsible for tumor progression and metastasis of advanced prostate cancer. In the present study, TGF-β1 was shown to induce VEGFA(165) secretion from both normal cell lines (HPV7 and RWPE1) and prostate cancer cell lines (DU145 and PC3). Conversely, hypoxia-stimulated VEGFA(165) secretion was observed only in prostate cancer cell lines. Hypoxia induced TGF-β1 expression in PC3 prostate cancer cells, and the TGF-β type I receptor (ALK5) kinase inhibitor partially blocked hypoxia-mediated VEGFA(165) secretion. This effect of hypoxia provides a novel mechanism to increase VEGFA expression in prostate cancer cells. Although autocrine signaling of VEGFA has been implicated in prostate cancer progression and metastasis, the associated mechanism is poorly characterized. VEGFA activity is mediated via VEGF receptor (VEGFR) 1 (Flt-1) and 2 (Flk-1/KDR). Whereas VEGFR-1 mRNA was detected in normal prostate epithelial cells, VEGFR-2 mRNA and VEGFR protein were expressed only in PC3 cells. VEGFA(165) treatment induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) in PC3 cells but not in HPV7 cells, suggesting that the autocrine function of VEGFA may be uniquely associated with prostate cancer. Activation of VEGFR-2 by VEGFA(165) was shown to enhance migration of PC3 cells. A similar effect was also observed with endogenous VEGFA induced by TGF-β1 and hypoxia. These findings illustrate that an autocrine loop of VEGFA via VEGFR-2 is critical for the tumorigenic effects of TGF-β1 and hypoxia on metastatic prostate cancers. | Base Sequence, Blotting, Western, Cell Hypoxia, DNA Primers, Humans, Male, Phosphorylation, Polymerase Chain Reaction, Prostate, Prostatic Neoplasms, RNA, Messenger, Signal Transduction, Transforming Growth Factor beta1, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1 | null |
22,705,565 | 2013-07-08 | 2013-11-21 | 1528-1175 | Anesthesiology | A boring Thanksgiving. | Black Ian H | eng | null | Journal Article | null | IM | 22705565, 10.1097/ALN.0b013e31825fae09 | null | Anesthesiology, Holidays, Humans, Iraq War, 2003-2011, Medicine in Literature, Physicians | null |
22,705,567 | 2012-10-15 | 2018-12-01 | 1528-1175 | Anesthesiology | What are we looking for? The question of resident selection. | Fleisher Lee A, Evers Alex S, Wiener-Kronish Jeanine, Ulatowski John A | eng | null | Editorial, Comment | null | IM | 22705567, 10.1097/ALN.0b013e31825fb1d5 | null | Anesthesiology, Female, Humans, Internship and Residency, Male, Personnel Selection, School Admission Criteria | null |
22,705,568 | 2013-05-20 | 2013-03-21 | 1528-1175 | Anesthesiology | Images in anesthesiology: Severe posterior thigh abscess as a complication of popliteal sciatic nerve catheter. | Maheshwari Ankit, George John Edward, Esa Wael Ali-Sakr, Turan Alparslan, Mounir-Soliman Loran | eng | null | Case Reports, Journal Article | null | IM | 22705568, 10.1097/ALN.0b013e318260ce02 | null | Abscess, Anesthesiology, Female, Humans, Middle Aged, Nerve Block, Sciatic Nerve, Staphylococcal Infections, Staphylococcus aureus, Thigh | null |
22,705,570 | 2012-10-15 | 2018-12-01 | 1528-1175 | Anesthesiology | Ultrasound versus fluoroscopy in image-guided pain treatment: use caution. | Buvanendran Asokumar, Rathmell James P | eng | null | Editorial, Comment | null | IM | 22705570, 10.1097/ALN.0b013e3182605e2a | null | Female, Humans, Male, Nerve Block, Ultrasonography, Interventional, Zygapophyseal Joint | null |
22,705,569 | 2012-10-15 | 2021-10-21 | 1528-1175 | Anesthesiology | Trigeminal nerve injury ErbB3/ErbB2 promotes mechanical hypersensitivity. | Ma Fei, Zhang Liping, Westlund Karin N | eng | P20 RR020145 (NCRR NIH HHS, United States); 2P20RR020145 (NCRR NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Protein Kinase Inhibitors, Quinazolines, Lapatinib, Receptor, ErbB-2, Receptor, ErbB-3 | IM | 22705569, 10.1097/ALN.0b013e3182604b2b, PMC3406246, NIHMS387255, 17321718, 19046966, 18501433, 11252954, 17557177, 16554459, 21319222, 15800203, 11056475, 17099708, 11883839, 21604994, 18845940, 20957456, 14555954, 15936882, 20122287, 16426998, 16481445, 18946301, 12648463, 11701590, 20214527, 19850946, 8182437, 20392965, 17348025, 2837713, 15936881, 2451683, 21092742, 9030624 | Chronic constriction injury of the trigeminal infraorbital nerve results in transient analgesia followed by whisker pad mechanical allodynia in rats. Neuregulin 1 expressed on axonal membranes binds receptor tyrosine kinase ErbB, promoting Schwann cell development and remyelination. This study investigated whether orofacial mechanical allodynia is signaled by ErbB3-ErbB2 heterodimers in injured nerves. | Animals, Blotting, Western, Disease Models, Animal, Hyperalgesia, Lapatinib, Male, Neuralgia, Pain Threshold, Protein Kinase Inhibitors, Quinazolines, Rats, Rats, Inbred F344, Receptor, ErbB-2, Receptor, ErbB-3, Trigeminal Nerve Injuries, Vibrissae | null |
22,705,571 | 2013-01-14 | 2012-09-03 | 1879-0003 | International journal of biological macromolecules | Study of polyacrylamide grafted starch based algal flocculation towards applications in algal biomass harvesting. | Banerjee Chiranjib, Gupta Pratibha, Mishra Sumit, Sen Gautam, Shukla Pratyoosh, Bandopadhyay Rajib | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acrylic Resins, polyacrylamide, Starch | IM | 22705571, S0141-8130(12)00227-9, 10.1016/j.ijbiomac.2012.06.011 | Microalgae may be the source of high amount of lipid and protein. It has the property for carbon dioxide sequestration, recycling and also can remove pollutants from wastewater. Using traditional methods, collection of algal biomass is either cost effective, time consuming or may be toxic due to use of chemical salts. The aim of this study is to harvest freshwater microalgae (Chlorella sp. CB4) biomass by using polymer. Polyacrylamide grafted starch (St-g-PAM) has been synthesized by microwave assisted method involving a synergism of microwave radiation and ceric ammonium nitrate (CAN) to initiate the grafting reaction. The synthesis was optimized in terms of CAN and monomer (acrylamide) concentration. The algal flocculation efficacy of all the grades of this graft copolymer was studied through standard 'Jar test' procedure. Effects of percentage grafting, pH and zeta potential on percentage recovery of algal biomass were thoroughly investigated. | Acrylic Resins, Biomass, Chlorella, Flocculation, Fresh Water, Microwaves, Starch | null |
22,705,572 | 2013-01-14 | 2015-11-19 | 1879-0003 | International journal of biological macromolecules | Development of an amperometric sulfite biosensor based on SO(x)/PBNPs/PPY modified ITO electrode. | Rawal Rachna, Pundir C S | eng | null | Journal Article | Ferrocyanides, Polymers, Pyrroles, Sulfites, Tin Compounds, polypyrrole, indium tin oxide, Sulfite Oxidase, ferric ferrocyanide | IM | 22705572, S0141-8130(12)00224-3, 10.1016/j.ijbiomac.2012.06.008 | A sulfite oxidase (SO(x)) (EC 1.8.3.1) purified from Syzygium cumini leaves was immobilized onto prussian blue nanoparticles/polypyrrole composite (PBNPs/PPY) electrodeposited onto the surface of indium tin oxide (ITO) electrode. An amperometric sulfite biosensor was fabricated using SO(x)/PBNPs/PPY/ITO electrode as working electrode, Ag/AgCl as standard and Pt wire as auxiliary electrode connected through a potentiostat. The working electrode was characterized by Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) before and after immobilization of SO(x). The biosensor showed optimum response within 2s, when operated at 20 mV s⁻¹ in 0.1M Tris-HCl buffer, pH 8.5 and at 35 °C. Linear range and minimum detection limit were 0.5-1000 μM and 0.12 μM (S/N=3) respectively. There was good correlation (r=0.99) between red wine samples sulfite value by standard DTNB method and the present method. The sensor was evaluated with 97% recovery of added sulfite in red wine samples and 2.2% and 4.3% within and between batch coefficients of variation respectively. The sensor was employed for determination of sulfite level in red and white wine samples. The enzyme electrode was used 200 times over a period of 3 months when stored at 4 °C. | Biosensing Techniques, Dielectric Spectroscopy, Electrochemistry, Electrodes, Electroplating, Ferrocyanides, Nanoparticles, Plant Leaves, Polymers, Pyrroles, Sulfite Oxidase, Sulfites, Surface Properties, Syzygium, Tin Compounds, Wine | null |
22,705,573 | 2013-01-14 | 2022-03-10 | 1879-0003 | International journal of biological macromolecules | Efficacy of tetracycline encapsulated O-carboxymethyl chitosan nanoparticles against intracellular infections of Staphylococcus aureus. | Maya S, Indulekha S, Sukhithasri V, Smitha K T, Nair Shantikumar V, Jayakumar R, Biswas Raja | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Biocompatible Materials, Capsules, Drug Carriers, Gels, O-carboxymethylchitosan, Chitosan, Tetracycline | IM | 22705573, S0141-8130(12)00225-5, 10.1016/j.ijbiomac.2012.06.009 | Intracellular bacterial infections are recurrent, persistent and are difficult to treat because of poor penetration and limited availability of antibiotics within macrophages and epithelial cells. We developed biocompatible, 200 nm sized tetracycline encapsulated O-carboxymethyl chitosan nanoparticles (Tet-O-CMC Nps) via ionic gelation for its sustained delivery of Tet into cells. S. aureus binds and aggregates with Tet-O-CMC Nps increasing drug concentrations at the infection site. Tet-O-CMC Nps were sixfold more effective in killing intracellular S. aureus compared to Tet alone in HEK-293 and differentiated THP1 macrophage cells proving it to be an efficient nanomedicine to treat intracellular S. aureus infections. | Anti-Bacterial Agents, Biocompatible Materials, Capsules, Chitosan, Drug Carriers, Endocytosis, Gels, HEK293 Cells, Hemolysis, Humans, Intracellular Space, Nanoparticles, Particle Size, Platelet Activation, Staphylococcus aureus, Tetracycline | null |
22,705,574 | 2014-02-24 | 2012-10-26 | 1879-0003 | International journal of biological macromolecules | Adsorption properties of gold onto a chitosan derivative. | Wang Lin, Peng Haiqing, Liu Song, Yu Huahua, Li Pengcheng, Xing Ronge | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Water, Gold, Chitosan | IM | 22705574, S0141-8130(12)00226-7, 10.1016/j.ijbiomac.2012.06.010 | In order to find a material which can be used for the recovery of Au(III), a chitosan derivative was synthesized by carboxymethylation and grafting sulfur groups onto cross-linked chitosan backbone. Adsorption studies were carried out at different pH values to optimize the pH condition. Batch method was conducted to study the effects of parameters such as reaction time, initial metal concentration and temperature on Au(III) sorption. The maximum adsorption affinity for Au(III) was found to be 8.32 mmol/g at pH 4.0, 25 °C. The results of kinetic study showed that the adsorption reaction followed the pseudo second order model. The derivative showed high adsorption ability and reusability toward Au(III). All results suggested that the chitosan derivative had potential to be utilized in the recovery of Au(III) from aqueous medium. | Adsorption, Chitosan, Gold, Hydrogen-Ion Concentration, Kinetics, Thermodynamics, Water | null |
22,705,575 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | Intraneural lipomatous tumor of the median nerve: Three case reports with a review of literature. | Okubo Taketo, Saito Tsuyoshi, Mitomi Hiroyuki, Takagi Tatsuya, Torigoe Tomoaki, Suehara Yoshiyuki, Katagiri Hirohisa, Murata Hideki, Takahashi Mitsuru, Ito Ichiro, Yao Takashi, Kaneko Kazuo | eng | null | Journal Article | null | null | 22705575, S2210-2612(12)00101-0, 10.1016/j.ijscr.2012.05.007, PMC3397293, 16248139, 3970300, 4301740, 893987, 3998425, 19554233, 18508348, 21119833, 7936133, 5077083, 7252115, 14251460, 18070639, 3351231, 9108225, 14251461 | Intraneural lipoma and fibrolipomatous hamartoma of the nerve are rare soft tissue tumors that most commonly occur in the forearm and the wrist, and particularly within the median nerve. When the lesions are large enough, they may cause progressive compression neuropathy. They are distinct entities each other with different clinical and radiological findings and thereby need different surgical treatments. | null | null |
22,705,576 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | Palmar metastasis of an adenocarcinoma of the esophago-gastric-junction: First case report. | Kamolz Lars-Peter, Stiglbauer Wolfgang, Längle Friedrich | eng | null | Journal Article | null | null | 22705576, S2210-2612(12)00099-5, 10.1016/j.ijscr.2012.05.005, PMC3397285, 16531705, 15990309, 3543107, 6654944, 12239679, 275346, 166784 | Hand metastasis represents only approximately 0.007-0.2% of all metastatic lesions. The most common origin of hand metastasis is the lung, which is approximately 50% of all cases, followed by breast and kidney. Hand metastasis from gastric or esophagic cancer is even much more rare. | null | null |
22,705,577 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | A case of thoracolithiasis diagnosed thoracoscopically. | Komatsu Teruya, Sowa Terumasa, Fujinaga Takuji | eng | null | Journal Article | null | null | 22705577, S2210-2612(12)00100-9, 10.1016/j.ijscr.2012.05.006, PMC3397284, 20160605, 22349539, 16247140, 10860288, 5663123, 21445827, 16977300 | Thoracolithiasis is quite rare with only 18 cases reported in the literature. | null | null |
22,705,578 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | Subtle mesenteric avulsion in a traumatic abdominal wall hernia: A case report. | Kordzadeh Ali, Devanesan Arjun, Parkinson Tim, Rahim Kiran, Panayiotopoulos Yiannis | eng | null | Journal Article | null | null | 22705578, S2210-2612(12)00098-3, 10.1016/j.ijscr.2012.04.020, PMC3397292, 19153644, 7762898, 2190330, 8331706, 22084611, 10697099, 21633587, 14011403, 17099509, 12575786, 6712323, 20440527 | Traumatic abdominal wall hernias (TAWHs) are uncommon and to date less than 50 cases have been reported in the literature. Furthermore mesenteric avulsion is a rare complication of TAWHs and occurs in less than 5% of all cases. | null | null |
22,705,580 | 2012-10-02 | 2021-10-21 | 2210-2612 | International journal of surgery case reports | Mesocaval shunt for portal hypertensive small bowel bleeding documented with intraoperative enteroscopy. | Symeonidis Dimitrios, Koukoulis Georgios, Christodoulidis Grigorios, Mamaloudis Ioannis, Chatzinikolaou Ioannis, Tepetes Konstantinos | eng | null | Journal Article | null | null | 22705580, S2210-2612(12)00096-X, 10.1016/j.ijscr.2012.05.004, PMC3397289, 20712058, 9219775, 18592149, 19651060, 20695721, 10213130, 19528878, 16936448, 14722853, 15920326, 18709480, 18435460, 18026837, 16644456, 1553940, 3528543 | Besides upper gastrointestinal tract, small bowel has also been implicated as a potential source of hemorrhage in patients with portal hypertension. | null | null |
22,705,579 | 2012-10-02 | 2024-04-17 | 2210-2612 | International journal of surgery case reports | Bronchial leiomyoma in a chemical warfare victim-a causative agent or an incidental finding: A case report. | Behesthirouy Samad, Kakaei Farzad, Azhough Ramin, Fakhrjou Ashraf | eng | null | Journal Article | null | null | 22705579, S2210-2612(12)00092-2, 10.1016/j.ijscr.2012.05.001, PMC3397295, 22031233, 19486919, 17646466, 21783698, 2153371, 23168470, 21335423, 8239818, 20085862, 19559099, 7955036 | Bronchial leiomyoma is one of the rarest benign tumors of the lower respiratory system, compromising less than 2% of reported benign pulmonary tumors. Chemical warfare is a known cause of chronic pulmonary diseases in soldiers who survives of the chemical wars. Most of these patients are chronically under treatment by respiratory drugs and acute exacerbations of their symptoms prompt for investigations for diagnosis of a new complication in these patients. | null | null |
22,705,582 | 2012-11-05 | 2013-11-21 | 1872-7972 | Neuroscience letters | Renal sympathetic nerve activity is increased in monosodium glutamate induced hyperadipose rats. | da Silva Mattos Alexandro Márcio, Xavier Carlos Henrique, Karlen-Amarante Marlusa, da Cunha Natália Veronez, Fontes Marco Antonio Peliky, Martins-Pinge Marli Cardoso | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Sodium Glutamate | IM | 22705582, S0304-3940(12)00817-8, 10.1016/j.neulet.2012.06.021 | The literature suggests that both obesity and hypertension are associated with increased sympathetic nerve activity. In the present study we evaluated the renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) in hyperadipose rats induced by neonatal administration of monosodium glutamate (MSG). Neonatal Wistar male rats were injected with MSG (4 mg/g body weight ID) or equimolar saline (control) for 5 days. At 90th day, all rats were anesthetized (urethane 1.4 g/kg) and prepared for MAP, HR and renal sympathetic nerve activity recordings. The anesthetized MSG rats presented baseline hypertension and increased baseline RSNA compared with control. Our results suggest the involvement of the renal sympathetic nervous system in the physiopathology of the MSG obesity. | Adiposity, Animals, Animals, Newborn, Blood Pressure, Heart Rate, Hypertension, Kidney, Male, Obesity, Rats, Rats, Wistar, Sodium Glutamate, Sympathetic Nervous System | null |
22,705,583 | 2013-01-23 | 2012-08-15 | 1873-7811 | Clinical psychology review | The potential of telemental health applications for obsessive-compulsive disorder. | Herbst Nirmal, Voderholzer Ulrich, Stelzer Nicola, Knaevelsrud Christine, Hertenstein Elisabeth, Schlegl Sandra, Nissen Christoph, Külz Anne Katrin | eng | null | Journal Article, Review | null | IM | 22705583, S0272-7358(12)00068-2, 10.1016/j.cpr.2012.04.005 | Only a small percentage of patients with obsessive-compulsive disorder (OCD) receive adequate treatment. Reasons include a high level of shame and stigmatisation and the paucity of specialised treatment services. Telemental health (TMH) treatment could improve the therapeutic situation as has been shown for various mental disorders. This review critically evaluates the current body of evidence on TMH applications for OCD patients. The review focuses on studies that include exposure therapy with response prevention as the best validated treatment component. | Bibliotherapy, Humans, Implosive Therapy, Obsessive-Compulsive Disorder, Self-Help Groups, Telemedicine, Therapy, Computer-Assisted | null |
22,705,581 | 2013-01-04 | 2021-10-21 | 1872-8057 | Molecular and cellular endocrinology | Glucocorticoid-induction of hypothalamic aromatase via its brain-specific promoter. | Brooks D C, Zhao H, Yilmaz M B, Coon V J S, Bulun S E | eng | R01 HD038691 (NICHD NIH HHS, United States); U54 HD040093 (NICHD NIH HHS, United States) | Journal Article, Research Support, American Recovery and Reinvestment Act, Research Support, N.I.H., Extramural | Glucocorticoids, Receptors, Glucocorticoid, Dexamethasone, Luciferases, Renilla, Aromatase | IM | 22705581, S0303-7207(12)00306-1, 10.1016/j.mce.2012.05.012, PMC3434699, NIHMS390401, 12604651, 8871783, 7608217, 2881624, 11600509, 17270396, 8923826, 8597600, 9238254, 2891134, 17406303, 14623533, 19393092, 9365213, 17341559, 14698071, 15083376, 3390233, 15288182, 6321146, 16191621, 21393445, 19605792, 21628418, 11744073, 16109840, 2494035, 2587988, 17120292, 10095084, 11606422, 18182446 | In the brain, a 36-kb distal promoter (I.f) regulates the Cyp19a1 gene that encodes aromatase, the key enzyme for estrogen biosynthesis. Local estrogen production in the brain regulates critical functions such as gonadotropin secretion and sexual behavior. The mechanisms that control brain aromatase production are not well understood. Here we show that the glucocorticoid dexamethasone robustly increases aromatase mRNA and protein by up to 98-fold in mouse hypothalamic cell lines in a dose- and time-dependent fashion. Using deletion mutants of the brain-specific promoter I.f and chromatin immunoprecipitation-PCR, we isolated a distinct region (-500/-200 bp) which becomes enriched in bound glucocorticoid receptor upon dexamethasone stimulation. A glucocorticoid antagonist or siRNA based knockdown of glucocorticoid receptor ablated dexamethasone stimulation of aromatase expression. Our findings demonstrate how glucocorticoids alter aromatase expression in the hypothalamus and might indicate a mechanism whereby glucocorticoid action modifies gonadotropin pulses and the menstrual cycle. | Animals, Aromatase, Cell Line, Dexamethasone, Enzyme Induction, Gene Knockdown Techniques, Genes, Reporter, Glucocorticoids, Hypothalamus, Luciferases, Renilla, Mice, Organ Specificity, Promoter Regions, Genetic, RNA Interference, Receptors, Glucocorticoid, Transcriptional Activation, Up-Regulation | null |
22,705,588 | 2012-11-30 | 2020-09-30 | 1873-2690 | Plant physiology and biochemistry : PPB | Oil body mobilization in sunflower seedlings is potentially regulated by thioredoxin h. | Babazadeh Nahid, Poursaadat Maral, Sadeghipour Hamid Reza, Colagar Abasalt Hossein Zadeh | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Plant Oils, Thioredoxins, Ascorbic Acid | IM | 22705588, S0981-9428(12)00127-1, 10.1016/j.plaphy.2012.05.013 | Thioredoxins are believed to mediate starch and protein mobilization in germinating cereals and dicotyledons. Nothing is known about redox regulation of lipid mobilization in plants. The possible redox regulation by thioredoxin h (Trx h) of a thiol-protease which degrades the oleosin coat of the oil body and its impacts on lipid mobilization was investigated in sunflower (Helianthus annuus L.) seedlings. An alkaline proteolytic activity stimulated by light was detected in seedlings. In vitro, the activity of this alkaline protease increased after reduction by NADPH-thiordoxin reductase system (NTS). The expression pattern of an alkaline 65 kDa thiol protease detected by gelatin SDS-PAGE technique, corresponded to the activity profile of the NTS-activated protease. The thiol-specific fuorochrome monobromobimane (mBBr) showed that a 65 kDa protein was also in a reduced state in vivo and becomes reduced in vitro by NTS. Except for 17-20 kDa oleosins, other oil body associated mBBr-labeled proteins were disappeared within three days following germination. Treatments of sunflower oil bodies by the NTS-activated alkaline protease made them more susceptible to maize lipase action. Ascorbate application enhanced lipid mobilization of seedlings. A model for seedling oil body mobilization was proposed according to which Trx h or other Trx isoforms, reductively activates an oleosin degrading thiol-protease and some oil body proteins, thus renders the organelle more susceptible to subsequent lipolytic actions. For the first time the potential role of Trx in the mobilization of lipid reserves in plants has been shown. | Ascorbic Acid, Electrophoresis, Polyacrylamide Gel, Helianthus, Lipid Metabolism, Plant Oils, Seedlings, Thioredoxins | null |
22,705,585 | 2012-09-26 | 2020-12-09 | 1090-2422 | Experimental cell research | Beneficial effects of curcumin on GFAP filament organization and down-regulation of GFAP expression in an in vitro model of Alexander disease. | Bachetti Tiziana, Di Zanni Eleonora, Balbi Pietro, Ravazzolo Roberto, Sechi GianPietro, Ceccherini Isabella | eng | null | Journal Article, Research Support, Non-U.S. Gov't | CRYAB protein, human, DNA Primers, Glial Fibrillary Acidic Protein, HSP27 Heat-Shock Proteins, HSPB1 protein, human, Heat-Shock Proteins, Molecular Chaperones, Mutant Proteins, Recombinant Fusion Proteins, alpha-Crystallin B Chain, Curcumin | IM | 22705585, S0014-4827(12)00287-X, 10.1016/j.yexcr.2012.06.008 | Heterozygous mutations of the GFAP gene are responsible for Alexander disease, a neurodegenerative disorder characterized by intracytoplasmic Rosenthal fibers (RFs) in dystrophic astrocytes. In vivo and in vitro models have shown co-localization of mutant GFAP proteins with the small heat shock proteins (sHSPs) HSP27 and alphaB-crystallin, ubiquitin and proteasome components. Results reported by several recent studies agree on ascribing an altered cytoskeletal pattern to mutant GFAP proteins, an effect which induces mutant proteins accumulation, leading to impaired proteasome function and autophagy induction. On the basis of the protective role shown by both these small heat shock proteins (sHSPs), and on the already well established neuroprotective effects of curcumin in several diseases, we have investigated the effects of this compound in an in vitro model of Alexander disease, consisting in U251-MG astrocytoma cells transiently transfected with a construct encoding for GFAP carrying the p.R239C mutation in frame with the reporter green fluorescent protein (GFP). In particular, depending on the dose used, we have observed that curcumin is able to induce both HSP27 and alphaB-crystallin, to reduce expression of both RNA and protein of endogenous GFAP, to induce autophagy and, finally, to rescue the filamentous organization of the GFAP mutant protein, thus suggesting a role of this spice in counteracting the pathogenic effects of GFAP mutations. | Alexander Disease, Base Sequence, Cell Line, Curcumin, DNA Primers, Down-Regulation, Glial Fibrillary Acidic Protein, HSP27 Heat-Shock Proteins, Heat-Shock Proteins, Humans, Molecular Chaperones, Mutant Proteins, Protein Multimerization, Recombinant Fusion Proteins, Up-Regulation, alpha-Crystallin B Chain | null |
22,705,586 | 2012-10-18 | 2018-12-01 | 1090-2422 | Experimental cell research | Comparative genomic and proteomic analysis of high grade glioma primary cultures and matched tumor in situ. | Howley R, Kinsella P, Buckley P G, Alcock L, Jansen M, Heffernan J, Stallings R L, Brett F M, Amberger-Murphy V, Farrell M A | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Biomarkers, Tumor, EGFR protein, human, ErbB Receptors, Receptor, Platelet-Derived Growth Factor alpha, Receptor, Platelet-Derived Growth Factor beta, PTEN Phosphohydrolase, PTEN protein, human | IM | 22705586, S0014-4827(12)00286-8, 10.1016/j.yexcr.2012.06.007 | Developing targeted therapies for high grade gliomas (HGG), the most common primary brain tumor in adults, relies largely on glioma cultures. However, it is unclear if HGG tumorigenic signaling pathways are retained under in-vitro conditions. Using array comparative genomic hybridization and immunohistochemical profiling, we contrasted the epidermal and platelet-derived growth factor receptor (EGFR/PDGFR) in-vitro pathway status of twenty-six primary HGG cultures with the pathway status of their original HGG biopsies. Genomic gains or amplifications were lost during culturing while genomic losses were more likely to be retained. Loss of EGFR amplification was further verified immunohistochemically when EGFR over expression was decreased in the majority of cultures. Conversely, PDGFRα and PDGFRβ were more abundantly expressed in primary cultures than in the original tumor (p<0.05). Despite these genomic and proteomic differences, primary HGG cultures retained key aspects of dysregulated tumorigenic signaling. Both in-vivo and in-vitro the presence of EGFR resulted in downstream activation of P70s6K while reduced downstream activation was associated with the presence of PDGFR and the tumor suppressor, PTEN. The preserved pathway dysregulation make this glioma model suitable for further studies of glioma tumorigenesis, however individual culture related differences must be taken into consideration when testing responsiveness to chemotherapeutic agents. | Adult, Aged, Biomarkers, Tumor, Brain Neoplasms, Comparative Genomic Hybridization, ErbB Receptors, Female, Genomics, Glioma, Humans, Immunoenzyme Techniques, In Situ Hybridization, Fluorescence, Male, Middle Aged, Neoplasm Grading, PTEN Phosphohydrolase, Proteomics, Receptor, Platelet-Derived Growth Factor alpha, Receptor, Platelet-Derived Growth Factor beta, Signal Transduction, Tumor Cells, Cultured, Young Adult | null |
22,705,584 | 2012-10-18 | 2021-10-21 | 1090-2422 | Experimental cell research | Changes in mitochondrial DNA alter expression of nuclear encoded genes associated with tumorigenesis. | Jandova Jana, Janda Jaroslav, Sligh James E | eng | P30 CA023074 (NCI NIH HHS, United States); R01 AR051552 (NIAMS NIH HHS, United States); R01AR0501552 (NIAMS NIH HHS, United States) | Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | 3-(4-methylphenylsulfonyl)-2-propenenitrile, DNA, Mitochondrial, Dipeptides, Matrix Metalloproteinase Inhibitors, N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide, NF-kappa B, Nitriles, Protease Inhibitors, Sulfones, Matrix Metalloproteinase 9 | IM | 22705584, S0014-4827(12)00285-6, 10.1016/j.yexcr.2012.06.006, PMC3418480, NIHMS393083, 11260590, 11861366, 10820020, 7569999, 17532310, 18362051, 9194590, 20564628, 20204677, 19352385, 20732728, 21912660, 19086058, 12844422, 12772087, 17517629, 18388260, 10863156, 17119872, 20602761, 22011905, 20950454, 18413815, 20725776, 9806551, 9012815, 8643997, 16374452, 15647368, 18421211, 12209135, 15728653, 16020738, 11865300, 16716161, 22119597, 12094550, 8834172, 11004456, 20028790, 10720328, 17761298, 18695616, 19796712 | We previously reported the presence of a mtDNA mutation hotspot in UV-induced premalignant and malignant skin tumors in hairless mice. We have modeled this change (9821insA) in murine cybrid cells and demonstrated that this alteration in mtDNA associated with mtBALB haplotype can alter the biochemical characteristics of cybrids and subsequently can contribute to significant changes in their behavioral capabilities. This study shows that changes in mtDNA can produce differences in expression levels of specific nuclear-encoded genes, which are capable of triggering the phenotypes such as seen in malignant cells. From a potential list of differentially expressed genes discovered by microarray analysis, we selected MMP-9 and Col1a1 for further studies. Real-time PCR confirmed up-regulation of MMP-9 and down-regulation of Col1a1 in cybrids harboring the mtDNA associated with the skin tumors. These cybrids also showed significantly increased migration and invasion abilities compared to wild type. The non-specific MMP inhibitor, GM6001, was able to inhibit migratory and invasive abilities of the 9821insA cybrids confirming a critical role of MMPs in cellular motility. Nuclear factor-κB (NF-κB) is a key transcription factor for production of MMPs. An inhibitor of NF-κB activation, Bay 11-7082, was able to inhibit the expression of MMP-9 and ultimately decrease migration and invasion of mutant cybrids containing 9821insA. These studies confirm a role of NF-κB in the regulation of MMP-9 expression and through this regulation modulates the migratory and invasive capabilities of cybrids with mutant mtDNA. Enhanced migration and invasion abilities caused by up-regulated MMP-9 may contribute to the tumorigenic phenotypic characteristics of mutant cybrids. | Animals, Apoptosis, Cell Movement, Cell Nucleus, Cell Transformation, Neoplastic, Cells, Cultured, DNA, Mitochondrial, Dipeptides, Matrix Metalloproteinase 9, Matrix Metalloproteinase Inhibitors, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mitochondria, Mutation, NF-kappa B, Nitriles, Phenotype, Protease Inhibitors, Real-Time Polymerase Chain Reaction, Skin Neoplasms, Sulfones | null |
22,705,587 | 2012-10-24 | 2021-10-21 | 1942-0080 | Circulation. Cardiovascular imaging | Normal left ventricular myocardial thickness for middle-aged and older subjects with steady-state free precession cardiac magnetic resonance: the multi-ethnic study of atherosclerosis. | Kawel Nadine, Turkbey Evrim B, Carr J Jeffrey, Eng John, Gomes Antoinette S, Hundley W Gregory, Johnson Craig, Masri Sofia C, Prince Martin R, van der Geest Rob J, Lima João A C, Bluemke David A | eng | ZIA EB000072-02 (Intramural NIH HHS, United States); N01HC95169 (NHLBI NIH HHS, United States); N01-HC-95159 (NHLBI NIH HHS, United States); N01-HC-95169 (NHLBI NIH HHS, United States); N01HC95159 (NHLBI NIH HHS, United States); ZIA CL090019-02 (Intramural NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural | Contrast Media, Gadolinium DTPA | IM | 22705587, CIRCIMAGING.112.973560, 10.1161/CIRCIMAGING.112.973560, PMC3412148, NIHMS394310, 2949575, 12450604, 17008985, 10595407, 3873849, 8496547, 1753157, 21193690, 18446346, 10756992, 7634452, 16516701, 9291041, 2305059, 16714609, 8133657, 12412033, 12397006, 11599059, 19589434, 2297833, 12594722, 15939807, 18581356, 11897450, 11815441 | Increased left ventricular myocardial thickness (LVMT) is a feature of several cardiac diseases. The purpose of this study was to establish standard reference values of normal LVMT with cardiac magnetic resonance and to assess variation with image acquisition plane, demographics, and left ventricular function. | Aged, Aged, 80 and over, Atherosclerosis, Cardiac-Gated Imaging Techniques, Contrast Media, Female, Gadolinium DTPA, Heart Ventricles, Humans, Image Interpretation, Computer-Assisted, Longitudinal Studies, Magnetic Resonance Imaging, Cine, Male, Middle Aged, Reference Values, Sex Factors, Ventricular Function, Left | null |
22,705,589 | 2012-11-30 | 2020-09-30 | 1873-2690 | Plant physiology and biochemistry : PPB | 24-epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: a shotgun approach. | Yusuf M, Fariduddin Q, Ahmad A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antioxidants, Brassinosteroids, Leghemoglobin, Steroids, Heterocyclic, Nickel, Catalase, Peroxidase, Superoxide Dismutase, Nitrogenase, Nitrate Reductase, Carbonic Anhydrases, brassinolide | IM | 22705589, S0981-9428(12)00109-X, 10.1016/j.plaphy.2012.05.004 | The objective of this study was to explore the response of 24-epibrassinolide to improve the biological yield of Ni-tolerant and Ni-sensitive varieties of Vigna radiata and also to test the propositions that 24-epibrassinolide induced up-regulation of antioxidant system protects the efficiency of V. radiata, grown under Ni-stress. Surface sterilized seeds of var. T-44 (Ni-tolerant) and PDM-139 (Ni-sensitive) were soaked in DDW (control), 10(-10), 10(-8), or 10(-6) M of 24-epibrassinolide for 8 h (shotgun approach). These treated seeds were then inoculated with specific Rhizobium grown in sandy loam soil supplemented with different levels of Ni 0, 50, 100, or 150 mg Ni kg(-1) of soil and were allowed to grow for 45-days. At this stage of growth, plants were sampled to assess the various growths and nodule related traits as well as selected biochemical characteristics. The remaining plants were allowed to grow to maturity to study the yield characteristics. The results indicated that plant-fresh and dry mass, number of nodules, their fresh and dry mass, leghemoglobin content, nitrogen and carbohydrate content in the nodules, leaf chlorophyll content, activities of nitrate reductase and carbonic anhydrase decreased proportionately with the increasing concentrations of soil nickel. However, the application of 24-epibrassinolide as shotgun approach (pre-sowing seed soaking) to the nickel-stressed or non-stressed plants improved growth, nodulation and enhanced the activity of various antioxidant enzymes (viz. catalase, peroxidase and superoxide dismutase) and also the content of proline. The up-regulation of antioxidant enzymes as well as proline (osmolyte) triggered by 24-epibrassinolide could have conferred tolerance to the Ni-stressed plants resulting in improved growth, nodulation and yield attributes. | Antioxidants, Brassinosteroids, Carbonic Anhydrases, Catalase, Fabaceae, Leghemoglobin, Nickel, Nitrate Reductase, Nitrogenase, Peroxidase, Plant Root Nodulation, Rhizobium, Root Nodules, Plant, Steroids, Heterocyclic, Superoxide Dismutase | null |
22,705,590 | 2012-11-30 | 2020-09-30 | 1873-2690 | Plant physiology and biochemistry : PPB | How do galactoglucomannan oligosaccharides regulate cell growth in epidermal and cortical tissues of mung bean seedlings? | Richterová-Kučerová Danica, Kollárová Karin, Zelko Ivan, Vatehová Zuzana, Lišková Desana | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Indoles, Mannans, galactoglucomannan, indolebutyric acid | IM | 22705590, S0981-9428(12)00128-3, 10.1016/j.plaphy.2012.05.014 | Biologically active galactoglucomannan oligosaccharides (GGMOs) alone or in combination with IBA stimulate primary root elongation and inhibit hypocotyl elongation in mung bean (Vigna radiata (L.) Wilczek) seedlings. For a more detailed view of GGMOs effect in these processes, the present work is focused on cell growth in selected tissues (epidermis and primary cortex) and on xylem formation. The GGMOs effect on tissue level has not been studied so far. The results show that GGMOs-induced stimulation of primary root growth is mainly caused by enhancing cell elongation (and in less extent by cell production rate) in all tissues observed. Xylem elements were formed at longer distance from the root tip than in the control. In hypocotyl GGMOs reduced cell elongation. IBA in roots caused decrease of cell elongation and cell production rate and acceleration of xylem maturation; in hypocotyls IBA strongly stimulated cell elongation. Application of GGMOs with IBA resulted in increase of cell elongation, cell production rate and delay of xylem maturation in roots. In GGMOs + IBA treated hypocotyls, cell length was decreased to 50% compared to IBA. Based on our results it can be concluded that GGMOs induced elongation growth in mung bean seedlings was caused by increased cell production rate and cell elongation and was accompanied with delay of xylem maturation. | Fabaceae, Hypocotyl, Indoles, Mannans, Plant Epidermis, Seedlings, Xylem | null |
22,705,592 | 2012-11-30 | 2020-09-30 | 1873-2690 | Plant physiology and biochemistry : PPB | Application of the split root technique to study iron uptake in cucumber plants. | De Nisi Patrizia, Vigani Gianpiero, Dell'Orto Marta, Zocchi Graziano | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Iron, Proton-Translocating ATPases, Phosphoenolpyruvate Carboxylase | IM | 22705592, S0981-9428(12)00136-2, 10.1016/j.plaphy.2012.05.022 | The regulation exerted by the Fe status in the plant on Fe deficiency responses was investigated in Cucumis sativus L. roots at both biochemical and molecular levels. Besides the two activities strictly correlated with Fe deficiency response, those of the Fe(III)-chelate reductase and the high affinity Fe transporter, we considered also H(+)-ATPase (EC 3.6.3.6) and phosphoenolpyruvate carboxylase (EC 4.1.1.31), that have been shown to be involved in this response. Both enzymatic activities and gene expression were monitored using a split root system. Absence of Fe induced the expression of the four transcripts, accompanied by an increase in the corresponding enzymatic activities. The application of the split root technique gave some information about the regulation of Fe uptake. In fact, 24 h after split root application, transcripts were still high and comparable to those of the -Fe control in the Fe-supplied half side, while in the -Fe side there was a drop in the expression and the relative enzymatic activities. Major changes occurred after 48 and 72 h. The coordinated regulation of these responses is discussed. | Cucumis sativus, Gene Expression Regulation, Plant, Iron, Phosphoenolpyruvate Carboxylase, Plant Roots, Proton-Translocating ATPases | null |
22,705,591 | 2012-11-30 | 2020-09-30 | 1873-2690 | Plant physiology and biochemistry : PPB | Thiamine primed defense provides reliable alternative to systemic fungicide carbendazim against sheath blight disease in rice (Oryza sativa L.). | Bahuguna Rajeev Nayan, Joshi Rohit, Shukla Alok, Pandey Mayank, Kumar J | eng | null | Journal Article | Benzimidazoles, Carbamates, Fungicides, Industrial, carbendazim, Thiamine | IM | 22705591, S0981-9428(12)00108-8, 10.1016/j.plaphy.2012.05.003 | A novel pathogen defense strategy by thiamine priming was evaluated for its efficacy against sheath blight pathogen, Rhizoctonia solani AG-1A, of rice and compared with that of systemic fungicide, carbendazim (BCM). Seeds of semidwarf, high yielding, basmati rice variety Vasumati were treated with thiamine (50 mM) and BCM (4 mM). The pot cultured plants were challenge inoculated with R. solani after 40 days of sowing and effect of thiamine and BCM on rice growth and yield traits was examined. Higher hydrogen peroxide content, total phenolics accumulation, phenylalanine ammonia lyase (PAL) activity and superoxide dismutase (SOD) activity under thiamine treatment displayed elevated level of systemic resistance, which was further augmented under challenging pathogen infection. High transcript level of phenylalanine ammonia lyase (PAL) and manganese superoxide dismutase (MnSOD) validated mode of thiamine primed defense. Though minimum disease severity was observed under BCM treatment, thiamine produced comparable results, with 18.12 per cent lower efficacy. Along with fortifying defense components and minor influence on photosynthetic pigments and nitrate reductase (NR) activity, thiamine treatment significantly reduced pathogen-induced loss in photosynthesis, stomatal conductance, chlorophyll fluorescence, NR activity and NR transcript level. Physiological traits affected under pathogen infection were found signatory for characterizing plant's response under disease and were detectable at early stage of infection. These findings provide a novel paradigm for developing alternative, environmentally safe strategies to control plant diseases. | Benzimidazoles, Carbamates, Fungicides, Industrial, Gene Expression Regulation, Plant, Oryza, Plant Diseases, Rhizoctonia, Thiamine | null |
22,705,595 | 2012-10-25 | 2022-03-10 | 1528-0020 | Blood | Clinical and prognostic role of annexin A2 in multiple myeloma. | Seckinger Anja, Meissner Tobias, Moreaux Jérôme, Depeweg Daniela, Hillengass Jens, Hose Katja, Rème Thierry, Rösen-Wolff Angela, Jauch Anna, Schnettler Reinhard, Ewerbeck Volker, Goldschmidt Hartmut, Klein Bernard, Hose Dirk | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | ANXA2 protein, human, Annexin A2, Biomarkers, Tumor, Receptors, Peptide, annexin II receptor, human | IM | 22705595, S0006-4971(20)46558-9, 10.1182/blood-2012-03-415588 | Annexin A2 (ANXA2) promotes myeloma cell growth, reduces apoptosis in myeloma cell lines, and increases osteoclast formation. ANXA2 has been described in small cohorts of samples as expressed by myeloma cells and cells of the BM microenvironment. To investigate its clinical role, we assessed 1148 samples including independent cohorts of 332 and 701 CD138-purified myeloma cell samples from previously untreated patients together with clinical prognostic factors, chromosomal aberrations, and gene expression-based high-risk scores, along with expression of ANXA2 in whole BM samples, stromal cells, osteoblasts, osteoclasts, and BM sera. ANXA2 is expressed in all normal and malignant plasma cell samples. Higher ANXA2 expression in myeloma cells is associated with significantly inferior event-free and overall survival independently of conventional prognostic factors and is associated with gene expression-determined high risk and high proliferation. Within the BM, all cell populations, including osteoblasts, osteoclasts, and stromal cells, express ANXA2. ANXA2 expression is increased significantly in myelomatous versus normal BM serum. ANXA2 exemplifies an interesting class of targetable bone-remodeling factors expressed by normal and malignant plasma cells and the BM microenvironment that have a significant impact on survival of myeloma patients. | Annexin A2, Biomarkers, Tumor, Bone Diseases, Case-Control Studies, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Genetic Association Studies, Humans, Multiple Myeloma, Prognosis, Receptors, Peptide, Risk Factors, Survival Analysis, Tumor Microenvironment, Validation Studies as Topic | null |
22,705,593 | 2012-10-16 | 2013-11-21 | 1096-0333 | Toxicology and applied pharmacology | Cytotoxic effects in 3T3-L1 mouse and WI-38 human fibroblasts following 72 hour and 7 day exposures to commercial silica nanoparticles. | Stępnik Maciej, Arkusz Joanna, Smok-Pieniążek Anna, Bratek-Skicki Anna, Salvati Anna, Lynch Iseult, Dawson Kenneth A, Gromadzińska Jolanta, De Jong Wim H, Rydzyński Konrad | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Silicon Dioxide, L-Lactate Dehydrogenase, Glutathione | IM | 22705593, S0041-008X(12)00261-X, 10.1016/j.taap.2012.06.002 | The potential toxic effects in murine (3T3-L1) and human (WI-38) fibroblast cell lines of commercially available silica nanoparticles (NPs), Ludox CL (nominal size 21 nm) and CL-X (nominal size of 30 nm) were investigated with particular attention to the effect over long exposure times (the tests were run after 72 h exposure up to 7 days). These two formulations differed in physico-chemical properties and showed different stabilities in the cell culture medium used for the experiments. Ludox CL silica NPs were found to be cytotoxic only at the higher concentrations to the WI-38 cells (WST-1 and LDH assays) but not to the 3T3-L1 cells, whereas the Ludox CL-X silica NPs, which were less stable over the 72 h exposure, were cytotoxic to both cell lines in both assays. In the clonogenic assay both silica NPs induced a concentration dependent decrease in the surviving fraction of 3T3-L1 cells, with the Ludox CL-X silica NPs being more cytotoxic. Cell cycle analysis showed a trend indicating alterations in both cell lines at different phases with both silica NPs tested. Buthionine sulfoximine (γ-glutamylcysteine synthetase inhibitor) combined with Ludox CL-X was found to induce a strong decrease in 3T3-L1 cell viability which was not observed for the WI-38 cell line. This study clearly indicates that longer exposure studies may give important insights on the impact of nanomaterials on cells. However, and especially when investigating nanoparticle effects after such long exposure, it is fundamental to include a detailed physico-chemical characterization of the nanoparticles and their dispersions over the time scale of the experiment, in order to be able to interpret eventual impacts on cells. | 3T3-L1 Cells, Animals, Cell Survival, Fibroblasts, Glutathione, Humans, L-Lactate Dehydrogenase, Mice, Nanoparticles, Oxidative Stress, Particle Size, Silicon Dioxide | null |
22,705,594 | 2012-10-16 | 2024-06-10 | 1096-0333 | Toxicology and applied pharmacology | Phototoxicity of nano titanium dioxides in HaCaT keratinocytes--generation of reactive oxygen species and cell damage. | Yin Jun-Jie, Liu Jun, Ehrenshaft Marilyn, Roberts Joan E, Fu Peter P, Mason Ronald P, Zhao Baozhong | eng | ZIA ES048012-13 (Intramural NIH HHS, United States); ZIA ES050139 (Intramural NIH HHS, United States); ZIA ES050139-17 (Intramural NIH HHS, United States) | Journal Article, Research Support, N.I.H., Intramural, Research Support, U.S. Gov't, P.H.S. | Reactive Oxygen Species, titanium dioxide, Titanium | IM | 22705594, S0041-008X(12)00260-8, 10.1016/j.taap.2012.06.001, PMC3407290, NIHMS391361, 20884039, 7809170, 21523480, 21121359, 21988075, 19501137, 8511786, 11778850, 19204862, 18554790, 16843823, 19695317, 21485823, 21121358, 18635669, 19618281, 12781626, 18491228, 2450098, 20528972, 17197137, 1559237, 18312389, 20348042, 21871579, 18986699, 10415838, 3002470, 16117799, 9703059, 21425910, 22115978, 9804429, 19223243, 972689, 21392107, 20183505, 9717713, 11522444 | Nano-sized titanium dioxide (TiO(2)) is among the top five widely used nanomaterials for various applications. In this study, we determine the phototoxicity of TiO(2) nanoparticles (nano-TiO(2)) with different molecular sizes and crystal forms (anatase and rutile) in human skin keratinocytes under UVA irradiation. Our results show that all nano-TiO(2) particles caused phototoxicity, as determined by the MTS assay and by cell membrane damage measured by the lactate dehydrogenase (LDH) assay, both of which were UVA dose- and nano-TiO(2) dose-dependent. The smaller the particle size of the nano-TiO(2) the higher the cell damage. The rutile form of nano-TiO(2) showed less phototoxicity than anatase nano-TiO(2). The level of photocytotoxicity and cell membrane damage is mainly dependent on the level of reactive oxygen species (ROS) production. Using polyunsaturated lipids in plasma membranes and human serum albumin as model targets, and employing electron spin resonance (ESR) oximetry and immuno-spin trapping as unique probing methods, we demonstrated that UVA irradiation of nano-TiO(2) can induce significant cell damage, mediated by lipid and protein peroxidation. These overall results suggest that nano-TiO(2) is phototoxic to human skin keratinocytes, and that this phototoxicity is mediated by ROS generated during UVA irradiation. | Blood Gas Monitoring, Transcutaneous, Blotting, Western, Cell Line, Dermatitis, Phototoxic, Electron Spin Resonance Spectroscopy, Enzyme-Linked Immunosorbent Assay, Humans, Keratinocytes, Lipid Peroxidation, Metal Nanoparticles, Reactive Oxygen Species, Titanium, Ultraviolet Rays | null |
22,705,599 | 2013-03-18 | 2017-11-16 | 1468-3318 | Tobacco control | The impact of Michigan's Dr Ron Davis smoke-free air law on levels of cotinine, tobacco-specific lung carcinogen and severity of self-reported respiratory symptoms among non-smoking bar employees. | Wilson Teri, Shamo Farid, Boynton Katherine, Kiley Janet | eng | 3U58DP001973-01S2 (NCCDPHP CDC HHS, United States) | Journal Article, Research Support, U.S. Gov't, P.H.S. | Nitrosamines, Pyridines, Tobacco Smoke Pollution, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butan-1-ol, Cotinine | IM | 22705599, tobaccocontrol-2011-050328, 10.1136/tobaccocontrol-2011-050328 | To determine the impact on bar employee's health and exposure to secondhand smoke (SHS) before and after the implementation of Michigan's Dr Ron Davis smoke-free air law that went into effect on 1 May 2010, prohibiting smoking in places of work, including bars. | Adult, Aged, Cotinine, Female, Follow-Up Studies, Humans, Male, Michigan, Middle Aged, Nitrosamines, Occupational Diseases, Occupational Exposure, Occupational Health, Pyridines, Respiratory Tract Diseases, Restaurants, Severity of Illness Index, Smoking, Smoking Prevention, Surveys and Questionnaires, Tobacco Smoke Pollution, Young Adult | null |
22,705,597 | 2012-12-03 | 2021-02-02 | 1528-0020 | Blood | Eyeing central neurons in vascular growth and reparative angiogenesis. | Sapieha Przemyslaw | eng | 221478 (Canadian Institutes of Health Research, Canada) | Journal Article, Research Support, Non-U.S. Gov't, Review | null | IM | 22705597, S0006-4971(20)46399-2, 10.1182/blood-2012-04-396846 | The generation of blood vessels is a highly synchronized process requiring the coordinated efforts of several vascular and nonvascular cell populations as well as a stringent orchestration by the tissue being vascularized. Stereotyped angiogenesis is vital for both developmental growth and to restore tissue metabolic supply after ischemic events. Central neurons such as those found in the brain, spinal cord, and retina are vast consumers of oxygen and nutrients and therefore require high rates of perfusion by functional vascular networks to ensure proper sensory transmission. During a metabolic mismatch, such as that occurring during a cerebrovascular infarct or in ischemic retinopathies, there is increasing evidence that central neurons have an inherent ability to influence the vascular response to injury. With a focus on the retina and retinal ischemic disorders, this review explores the ever-growing evidence suggesting that central neurons have the propensity to impact tissue vascularization and reparative angiogenesis. Moreover, it addresses the paradoxical ability of severely ischemic neurons to hinder vascular regrowth and thus segregate the most severely injured zones of nervous tissue. The topics covered here are pertinent for future therapeutic strategies because promoting and steering vascular growth may be beneficial for ischemic disorders. | Animals, Central Nervous System, Embryonic Development, Humans, Ischemia, Microvessels, Models, Biological, Neovascularization, Physiologic, Nerve Net, Nerve Regeneration, Neurons, Retina, Retinal Neovascularization, Retinal Vessels | null |
22,705,600 | 2014-05-12 | 2022-01-29 | 1468-3318 | Tobacco control | Knowledge of the health impacts of smoking and public attitudes towards tobacco control in the former Soviet Union. | Roberts Bayard, Stickley Andrew, Gilmore Anna B, Danishevski Kirill, Kizilova Kseniya, Bryden Anna, Rotman David, Haerpfer Christian, McKee Martin | eng | MR/K023195/1 (Medical Research Council, United Kingdom); R01 CA160695 (NCI NIH HHS, United States) | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705600, 10.1136/tobaccocontrol-2011-050249, tobaccocontrol-2011-050249, PMC3919033, NIHMS536739, 11850361, 9681906, 17565141, 20063489, 16046703, 21676953, 15175530, 10813022, 15175532, 16565462, 12612368, 12777433, 17897987, 18048621, 22594739, 18653793 | To describe levels of knowledge on the harmful effects of tobacco and public support for tobacco control measures in nine countries of the former Soviet Union and to examine the characteristics associated with this knowledge and support. | Adolescent, Adult, Comprehension, Cross-Sectional Studies, Female, Government, Health Knowledge, Attitudes, Practice, Health Policy, Humans, Logistic Models, Male, Middle Aged, Public Opinion, Smoking, Smoking Cessation, Socioeconomic Factors, Tobacco Use Disorder, USSR, Young Adult | Tobacco, commonwealth of independent states, low-/middle-income countries, public opinion, public policy |
22,705,601 | 2014-05-12 | 2013-10-17 | 1468-3318 | Tobacco control | Partner cigarette smoking and risk of neural tube defects among infants of non-smoking women in northern China. | Li Zhiwen, Zhang Le, Ye Rongwei, Liu Jianmeng, Pei Lijun, Zheng Xiaoying, Ren Aiguo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Tobacco Smoke Pollution | IM | 22705601, tobaccocontrol-2011-050384, 10.1136/tobaccocontrol-2011-050384 | To investigate the effect of secondhand smoke exposure from a partner on the risk of having a newborn baby with neural tube defects (NTDs) in Chinese non-smoking women. | Adolescent, Adult, Case-Control Studies, China, Female, Fetus, Humans, Infant, Infant, Newborn, Interviews as Topic, Male, Maternal Exposure, Neural Tube Defects, Odds Ratio, Pregnancy, Risk Factors, Smoking, Spouses, Tobacco Smoke Pollution, Young Adult | Partner smoking, low/middle-income country, neural tube defects, secondhand smoke, smoking-caused disease, socioeconomic status |
22,705,596 | 2012-11-27 | 2021-02-02 | 1528-0020 | Blood | CD86+ or HLA-G+ can be transferred via trogocytosis from myeloma cells to T cells and are associated with poor prognosis. | Brown Ross, Kabani Karieshma, Favaloro James, Yang Shihong, Ho P Joy, Gibson John, Fromm Phillip, Suen Hayley, Woodland Narelle, Nassif Najah, Hart Derek, Joshua Douglas | eng | null | Journal Article, Research Support, Non-U.S. Gov't | B7-2 Antigen, Biomarkers, HLA-G Antigens | IM | 22705596, S0006-4971(20)46433-X, 10.1182/blood-2012-03-416792 | The transfer of membrane proteins between cells during contact, known as trogocytosis, can create novel cells with a unique phenotype and altered function. We demonstrate that trogocytosis is more common in multiple myeloma (MM) than chronic lymphocytic leukemia and Waldenstrom macroglobulinaemia; that T cells are more probable to be recipients than B or natural killer cells; that trogocytosis occurs independently of either the T-cell receptor or HLA compatibility; and that after trogocytosis, T cells with acquired antigens can become novel regulators of T-cell proliferation. We screened 168 patients with MM and found that CD86 and human leukocyte antigen G (HLA-G) were antigens commonly acquired by T cells from malignant plasma cells. CD3+ CD86acq+ and CD3+ HLA-Gacq+ cells were more prevalent in bone marrow than peripheral blood samples. The presence of either CD86 or HLA-G on malignant plasma cells was associated with a poor prognosis. CD38++ side population cells expressed HLA-G, suggesting that these putative myeloma stem cells could generate immune tolerance. HLA-G+ T cells had a regulatory potency similar to natural Tregs, thus providing another novel mechanism for MM to avoid effective immune surveillance. | B-Lymphocytes, B7-2 Antigen, Biomarkers, Cell Membrane, Cell Proliferation, HLA-G Antigens, Humans, Immune Tolerance, Killer Cells, Natural, Leukemia, Lymphocytic, Chronic, B-Cell, Multiple Myeloma, Organ Specificity, Plasma Cells, Prognosis, Protein Transport, Survival Rate, T-Lymphocytes, Waldenstrom Macroglobulinemia | null |
22,705,598 | 2012-10-31 | 2021-02-02 | 1528-0020 | Blood | How I manage venous thromboembolism risk in hospitalized medical patients. | Dobromirski Mark, Cohen Alexander T | eng | null | Case Reports, Journal Article, Review | Anticoagulants, Estrogens, Fibrin Fibrinogen Degradation Products, fibrin fragment D | IM | 22705598, S0006-4971(20)49094-9, 10.1182/blood-2012-03-378901 | Venous thromboembolism is a significant cause of illness and death worldwide. Large bodies of evidence support the heightened risk status of hospitalized medical patients, and that prophylactic measures significantly reduce the risk of thrombosis, yet these patients often fail to receive adequate prophylactic therapy. This failure may be accounted for by a lack of awareness of the relevant indications, poorly designed implementation systems, and clinical concerns over the side effects of anticoagulant medications. This article briefly summarizes our understanding of the clinical factors relevant to the evaluation of venous thromboembolism risk in hospitalized medical patients. We describe our approach to the use of thromboprophylaxis, through which we aim to minimize the disease burden of this under-recognized and preventable pathology. | Adult, Age Factors, Aged, Anticoagulants, Contraindications, Estrogens, Female, Fibrin Fibrinogen Degradation Products, Humans, Immobilization, Kidney Failure, Chronic, Neoplasms, Obesity, Risk Factors, Thrombophilia, Varicose Veins, Venous Thromboembolism | null |
22,705,602 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | A molecular analysis of familial Mediterranean fever disease in a cohort of Turkish patients. | Dundar Munis, Kiraz Aslihan, Emirogullari Elif Funda, Saatci Ccedil Etin, Taheri Serpil, Baskol Mevlut, Polat Seher, Ozkul Yusuf | eng | null | Journal Article | Cytoskeletal Proteins, MEFV protein, human, Pyrin, DNA | IM | 22705602, 12-343, 10.5144/0256-4947.2012.343, PMC6081005, 19466978, 11085810, 18662100, 16283319, 19489440, 19115056, 12077709, 10799634, 16564365, 11464238, 10905662, 18353061, 16931648, 11053071, 15643295, 19091621, 19449169, 11470495, 19641922, 18266121, 10234504, 20485448, 15854197, 17568393, 11528510, 15020340, 16627024, 18000697, 10662876 | Familial Mediterranean fever (FMF) is an autosomal recessive disorder caused by mutations in MEFV gene, which encodes pyrin. FMF is especially prevalent among Turks, Armenians, non-Ashkenazi Jews, and Arabs. The aim of this study was to determine the frequency and spectrum of 12 MEFV mutations of these patients and any genotype-phenotype correlation in this large Turkish group. | Adolescent, Adult, Aged, Amyloidosis, Child, Child, Preschool, Cohort Studies, Consanguinity, Cytoskeletal Proteins, DNA, Familial Mediterranean Fever, Female, Gene Frequency, Genotype, Heterozygote, Homozygote, Humans, Infant, Male, Middle Aged, Mutation, Nucleic Acid Hybridization, Polymerase Chain Reaction, Pyrin, Retrospective Studies, Turkey, Young Adult | null |
22,705,603 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Association of interleukin 4 (-590 T/C) and interleukin 4 receptor (Q551R A/G) gene polymorphisms with acne vulgaris. | Al Robaee Ahmad A, AlZolibani Abdullateef, Al Shobaili Hani, Settin Ahmad | eng | null | Journal Article | Receptors, Interleukin-4, Interleukin-4 | IM | 22705603, 12-349, 10.5144/0256-4947.2012.349, PMC6081023, 10358772, 18624881, 17295710, 18615253, 15098062, 12485434, 9620765, 16702603, 15556722, 9034722, 10586888, 11390419, 14626815, 2018830, 15679586, 9579234, 9557256, 9856949, 20725545, 19218788, 10946814, 16484821, 8590350, 15634931, 10590262, 17900677, 8893001, 9392697, 12566802, 10677312, 9070874, 7642750, 20861605, 12689929, 11719504, 9575675, 8543789, 17033191, 10233717, 12695491, 10652462, 20650774, 18263811 | Acne vulgaris is a common skin disorder. The complete etiology of this disease remains to be identified; however, it seems that aberrant expression of cytokine genes might be a contributing factor. This study aimed to investigate the association of genetic polymorphisms related to interleukin 4 (IL-4) promotor and receptor (IL-4R) genes as inflammatory modulators with acne vulgaris. | Acne Vulgaris, Adolescent, Case-Control Studies, Female, Genetic Predisposition to Disease, Genotype, Humans, Interleukin-4, Male, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Receptors, Interleukin-4, Saudi Arabia, Severity of Illness Index, Young Adult | null |
22,705,607 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Incidence of ventricular arrhythmia and associated patient outcomes in hospitalized acute coronary syndrome patients in Saudi Arabia: findings from the registry of the Saudi Project for Assessment of Acute Coronary Syndrome (SPACE). | Hersi Ahmad S, Alhabib Khalid F, AlFaleh Hussam F, AlNemer Khalid, AlSaif Shukri, Taraben Amir, Kashour Tarek, Abuosa Ahmed M, Al-Murayeh Mushabab A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705607, 12-372, 10.5144/0256-4947.2012.372, PMC6081010, 20376186, 12119245, 83793, 16084178, 14631474, 12383588, 17933995, 18431887, 19584982, 8239250, 19417195, 19064008, 15660036, 19952468, 19420808, 19843923, 9843464, 15358045 | Mortality in acute coronary syndrome (ACS) patients with ventricular arrhythmia (VA) has been shown to be higher than those without VA. However, there is a paucity of data on VA among ACS patients in the Middle Eastern countries. | Acute Coronary Syndrome, Adult, Aged, Blood Pressure, Female, Hospital Mortality, Hospitalization, Hospitals, Public, Humans, Hyperlipidemias, Incidence, Male, Middle Aged, Myocardial Infarction, Prognosis, Prospective Studies, Registries, Risk Factors, Saudi Arabia, Sex Factors, Tachycardia, Ventricular, Treatment Outcome, Ventricular Fibrillation | null |
22,705,605 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Intracranial germ cell tumors: a single-institution experience. | Khafaga Yasser, El Weshi Amr, Nazmy Mohamed, Hassounah Maher, Alshail Esam, Moussa Emad, Allam Ayman, Alkofide Amani, Jamshed Arif, ELhusseiny Gamal, Ezzat Ibrahim, Jenkin Derek | eng | null | Journal Article | Antineoplastic Agents, Biomarkers, Tumor | IM | 22705605, 12-359, 10.5144/0256-4947.2012.359, PMC6081024, 9624246, 10561326, 11821471, 10234702, 2991485, 16970236, 17906212, 14990640, 9308946, 10098759, 8208347, 15992700, 11767296, 12046745, 3403311, 18513888, 8952565, 12738328, 11352956, 9053482, 7947108 | Intracranial germ cell tumors (GCTs) are not a common disease. We reviewed the experience of a single institution to determine the variables that affect treatment outcome. | Adolescent, Adult, Age Factors, Antineoplastic Agents, Biomarkers, Tumor, Brain Neoplasms, Child, Child, Preschool, Combined Modality Therapy, Disease-Free Survival, Female, Follow-Up Studies, Humans, Male, Neoplasm Recurrence, Local, Neoplasms, Germ Cell and Embryonal, Radiation Dosage, Retrospective Studies, Saudi Arabia, Survival Rate, Time Factors, Treatment Outcome, Young Adult | null |
22,705,604 | 2012-11-07 | 2021-12-03 | 0975-4466 | Annals of Saudi medicine | De novo malignancies after liver transplantation: a single-center experience. | Hegab Bassem, Khalaf Hatem, Allam Naglaa, Azzam Ayman, Al Khail Faisal Aba, Al-hamoudi Waleed, Kamel Yasser, Al Bahili Hamad, Al Sofayan Mohammed, Al-Sebayel Mohammed | eng | null | Journal Article | Antibodies, Monoclonal, Antigens, CD20, Immunosuppressive Agents | IM | 22705604, 12-355, 10.5144/0256-4947.2012.355, PMC6081011, 11959293, 19766646, 12004350, 9825817, 12570889, 11689783, 11552217, 12004349, 18283144, 15690541, 11211912, 6142304, 15094218, 11150415, 19663894, 16968488, 19460546, 9346628, 10083569, 7725508, 19741469, 19877025, 19715947, 11923703, 15023152, 7922092 | The recipients of liver transplantation (LT) are subjected to lifelong immunosuppression with its many drawbacks. De novo and recurrent malignancy in transplant recipients are attributed to attenuation of immunosurveillance. In the present study, we present our experience with de novo malignancies encountered after both deceased and living donor liver transplantations. | Adolescent, Adult, Aged, Antibodies, Monoclonal, Antigens, CD20, Child, Child, Preschool, Epstein-Barr Virus Infections, Female, Humans, Immunosuppression Therapy, Immunosuppressive Agents, Infant, Liver Transplantation, Lymphoproliferative Disorders, Male, Middle Aged, Neoplasms, Retrospective Studies, Survival Rate, Young Adult | null |
22,705,606 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Are acute coronary syndrome patients admitted during off-duty hours treated differently? An analysis of the Saudi Project for Assessment of Acute Coronary Syndrome (SPACE) study. | Al Faleh Hussam F, Thalib Lukman, AlHabib Khalid F, Ullah Anhar, AlNemer Khalid, AlSaif Shukri M, Taraben Amir N, Malik Asif, Abuosa Ahmed M, Mimish Layth A, Hersia Ahmad Salah | eng | null | Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't | Fibrinolytic Agents | IM | 22705606, 12-366, 10.5144/0256-4947.2012.366, PMC6081017, 16844875, 12777318, 16106005, 12070105, 19584982, 17360988, 10577442, 12777317, 18312751, 11738323, 15342297 | It is often suggested that acute coronary syndrome (ACS) patients admitted during off-duty hours (OH) have a worse clinical outcome than those admitted during regular working hours (RH). Our objective was to compare the management and hospital outcomes of ACS patients admitted during OH with those admitted during RH. | Acute Coronary Syndrome, Adult, After-Hours Care, Aged, Angioplasty, Balloon, Coronary, Delivery of Health Care, Female, Fibrinolytic Agents, Heart Failure, Hospital Mortality, Hospitalization, Humans, Male, Middle Aged, Myocardial Infarction, Personnel Staffing and Scheduling, Prospective Studies, Saudi Arabia, Time Factors, Treatment Outcome | null |
22,705,608 | 2012-11-07 | 2022-03-10 | 0975-4466 | Annals of Saudi medicine | The relationship between coronary artery calcification and myocardial perfusion in asymptomatic women. | Fathala Ahmed, Al Amer Ali, Shukri Mohamed, Abouzied Mohei M, Alsugair Abdulaziz | eng | null | Journal Article | null | IM | 22705608, 12-378, 10.5144/0256-4947.2012.378, PMC6081006, 11591627, 15120828, 10645919, 9180117, 10879414, 12975245, 4835188, 9014977, 14650385, 12569338, 11815441, 9315532, 10908208, 9091522, 5505449, 9741510, 11788211, 6980062, 21304416, 20552312, 10479662, 2407762, 15312881, 453039, 15687114, 11345357, 9426030, 11472697, 7554196, 10483960 | No data are available in Saudi Arabia on the relationship between coronary artery calcification (CAC) and myocardial perfusion scintigraphy (MPS) in asymptomatic women, for determining subclinical coronary artery disease (CAD). The main objective of this study was to investigate the relationship between the presence of CAC and stress-induced myocardial ischemia by MPS in asymptomatic women. | Adult, Aged, Calcinosis, Coronary Artery Disease, Exercise Test, Female, Humans, Middle Aged, Myocardial Ischemia, Myocardial Perfusion Imaging, Prevalence, Retrospective Studies, Risk Factors, Severity of Illness Index | null |
22,705,610 | 2012-11-07 | 2024-11-11 | 0975-4466 | Annals of Saudi medicine | The relationship between markedly elevated glucose challenge test results and the rate of gestational diabetes mellitus and gestational impaired glucose tolerance. | Karcaaltincaba Deniz, Altinbas Sadiman, Akyol Mesut, Ensari Tugba, Yalvac Serdar | eng | null | Journal Article | Blood Glucose | IM | 22705610, 12-391, 10.5144/0256-4947.2012.391, PMC6081012, 12017686, 17127496, 16157108, 17192378, 17376185, 19465234, 510803, 12576264, 8355957, 18067985, 7148898, 12027921, 12196438, 8972407, 8377974, 19464683, 16647890, 1680070, 14166677, 17403413, 15280108, 8299450, 11547793 | Even though the 50 g oral glucose challenge test (GCT) is the most commonly used screening modality for gestational diabetes mellitus (GDM), no consensus for the diagnostic approach is available to patients with a markedly elevated GCT result. We aimed to evaluate the diagnostic utility of markedly elevated GCT results and the impact of age using the oral glucose tolerance test (OGTT) as gold standard. | Adult, Age Factors, Blood Glucose, Diabetes, Gestational, Female, Glucose Intolerance, Glucose Tolerance Test, Humans, Predictive Value of Tests, Pregnancy, Retrospective Studies, Turkey, Young Adult | null |
22,705,611 | 2012-11-07 | 2024-03-30 | 0975-4466 | Annals of Saudi medicine | Health-related quality of life of Saudi hepatitis B and C patients. | Abdo Ayman A | eng | null | Journal Article | null | IM | 22705611, 12-397, 10.5144/0256-4947.2012.397, PMC6081013, 8749620, 7542854, 18318043, 12375144, 10421667, 1653818, 17845541, 9862876, 8596551, 9305658, 9672326, 9425939, 9862878, 10622597, 15565610, 19568517 | Although usually asymptomatic, many chronic hepatitis C patients have extrahepatic manifestations and impaired health-related quality of life (HRQOL), while hepatitis B virus (HBV) patients have normal or nearly normal HRQOL. The aim of this study was to investigate HRQOL in Saudi patients infected with hepatitis C virus (HCV) in comparison with patients infected with HBV in an effort to document the prevalence of and find factors associated with reduced HRQOL in these patients. | Adult, Female, Hepatitis B, Hepatitis C, Chronic, Humans, Male, Middle Aged, Prevalence, Prospective Studies, Quality of Life, Saudi Arabia, Surveys and Questionnaires, Young Adult | null |
22,705,616 | 2012-11-07 | 2022-03-18 | 0975-4466 | Annals of Saudi medicine | Anti-NMDA-receptor encephalitis presenting as postpartum psychosis in a young woman, treated with rituximab. | Shaaban Hamid Salim, Choo Hoo Feng, Sensakovic John W | eng | null | Case Reports, Journal Article | Antibodies, Monoclonal, Murine-Derived, Antigens, CD20, Immunologic Factors, Rituximab | IM | 22705616, 12-421, 10.5144/0256-4947.2012.421, PMC6081009, 18442127, 17479076, 19833974, 19364930, 17308294, 17262855, 21060097, 19324868, 19718525, 18851928, 20016378, 17431543, 19670433, 17898324, 18299525, 19047564, 16178029 | A severe paraneoplastic form of acute encephalitis associated with antibodies against the N-methyl D-aspartate (NMDA) receptor typically occurs in young individuals and is associated, but not always, with an underlying tumor. If diagnosed early, initiation of immunotherapy and tumor removal (if present) may result in recovery. We report a case in a 25-year-old young woman who presented to our medical center with postpartum psychosis. Treatment with rituximab (a chimeric monoclonal antibody against the protein CD20) resulted in gradual improvement in mental status and resolution of seizure activity episodes. A year after diagnosis and treatment, the patient was doing well without recurrences, and no tumors appeared. This is the first described case of anti-NMDA-receptor antibodies encephalitis that presented initially as a postpartum psychosis disorder and was successfully treated with rituximab. | Adult, Anti-N-Methyl-D-Aspartate Receptor Encephalitis, Antibodies, Monoclonal, Murine-Derived, Antigens, CD20, Female, Follow-Up Studies, Humans, Immunologic Factors, Postpartum Period, Psychotic Disorders, Rituximab, Treatment Outcome | null |
22,705,618 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Revisiting fetus-in-fetu. | Gude Dilip, Rayudu Batta Ramasubba, Bansal Dharam, Sashidhar Chennamsetty | eng | null | Case Reports, Journal Article, Review | null | IM | 22705618, 12-427, 10.5144/0256-4947.2012.427, PMC6081007, 13799033, 10835078, 5528247, 2869477, 12783257, 5891759, 6526492, 15405669, 14825068, 8233707, 5010721, 4673613, 3045784, 1151583, 19881090 | Fetus-in-fetu is a rare congenital anomaly in which a malformed parasitic twin is found within the body of its partner. Less than 100 cases have been reported in published studies. Although it is a relatively benign condition, clinicians need to have a high index of suspicion for the associated complications that may arise. We report the case of an infant presenting with jaundice and steadily growing abdominal mass, who was diagnosed with fetus-in-fetu syndrome. We review the published studies and discuss the pathophysiology, complexities, and management options. | Female, Fetus, Humans, Infant, Jaundice, Male, Pregnancy | null |
22,705,617 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Takotsubo cardiomyopathy resembling acute high lateral myocardial infarction. | Ilhan Erkan, Gurkan Ufuk, Yilmaz Hale Yaka, Guvenc Tolga Sinan, Canga Yigit, Bolca Osman | eng | null | Case Reports, Journal Article | null | IM | 22705617, 12-424, 10.5144/0256-4947.2012.424, PMC6081026, 16901561, 18045705, 15276100, 20093944, 11451258, 19712987, 19462813, 20859840, 19998697, 17175045, 15687136, 15703419, 21131373, 17067626 | A 65-year-old female patient admitted to the emergency department was diagnosed with acute high lateral myocardial infarction, but later Takotsubo cardiomyopathy (TC) was discovered. She had squeezing chest pain that started shortly after an emotional stress. The electrocardiogram revealed a loss of R wave voltage in leads V1 to V4 and an ST-segment elevation in I and aVL. After an urgent coronary angiography and ventriculography, TC was considered, and supportive anti-ischemic treatment was started. The severe left ventricular systolic dysfunction improved and normalized during the follow-up. She was discharged without any complications. TC is a new entity of acute cardiac events, and patients usually recover completely without sequelae with proper diagnosis and management. An exact diagnosis may also prevent an inappropriate application in the setting of recurrences. | Aged, Chest Pain, Electrocardiography, Female, Follow-Up Studies, Humans, Myocardial Infarction, Severity of Illness Index, Stress, Psychological, Takotsubo Cardiomyopathy, Ventricular Dysfunction, Left | null |
22,705,609 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Relationship between obstructive sleep apnea severity index and left ventricular function and volume. | Altintas Nejat, Aslan Ekrem, Helvaci Aysen, Malhotra Atul | eng | K24 HL093218 (NHLBI NIH HHS, United States); P01 HL095491 (NHLBI NIH HHS, United States); R01 HL085188 (NHLBI NIH HHS, United States); R01 HL090897 (NHLBI NIH HHS, United States) | Journal Article | null | IM | 22705609, 12-384, 10.5144/0256-4947.2012.384, PMC3821963, NIHMS399943, 2698218, 1503353, 9083973, 8558414, 14551166, 7592221, 9543265, 7781343, 10678860, 10908222, 8418995, 19413140, 11040224, 18725495, 7396006, 16055877, 11751180, 11399711, 17563527, 20308745, 19101028, 20423584, 10805822, 21104045, 12034649, 15589639, 12377833, 20381666, 19786163, 10940788, 12581679, 9488212, 1798888, 10450601, 17356087, 11208615, 7560081 | Obstructive sleep apnea (OSA) causes increased cardiovascular morbidity and mortality, including systemic arterial hypertension, coronary heart disease, heart rhythm and conduction disorders, heart failure and stroke. In our study, we aimed to assess left ventricular mass and myocardial performance index (MPI) in OSA patients. | Adult, Cross-Sectional Studies, Echocardiography, Doppler, Female, Humans, Male, Middle Aged, Myocardial Contraction, Polysomnography, Severity of Illness Index, Sleep Apnea, Obstructive, Turkey, Ventricular Dysfunction, Left, Ventricular Function, Left | null |
22,705,613 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Puberty development among children and adolescents with chronic disease in Saudi Arabia. | AlBuhairan Fadia, Tamimi Waleed, Tamim Hani, Al Mutair Angham, Felimban Naila, Altwaijri Yasmin, Shoukri Mohamed, Al Alwan Ibrahim | eng | null | Journal Article | null | IM | 22705613, 12-408, 10.5144/0256-4947.2012.408, PMC6081014, 8366901, 8464668, 11987904, 23761992, 20117793, 20159870, 12065928, 7506774, 11483799, 1832223, 10757202, 11987900, 3098185, 18340463, 1536351 | Increasing numbers of children with chronic health conditions are now surviving into adolescence and adulthood because of advancing health care. These chronic health conditions are generally known to impact a child's growth and development, including pubertal development. In Saudi Arabia, chronic diseases are prevalent, yet no reports of pubertal onset and its relation to chronic illness are available. The aim of this study was to explore pubertal development among Saudi children and adolescents with a chronic illness. | Adolescent, Child, Chronic Disease, Cross-Sectional Studies, Female, Humans, Male, Obesity, Overweight, Puberty, Saudi Arabia, Sex Characteristics | null |
22,705,624 | 2012-12-28 | 2013-11-21 | 1873-5169 | Peptides | Importance of the disulfide bridges in the antibacterial activity of human hepcidin. | Hocquellet Agnès, le Senechal Caroline, Garbay Bertrand | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Disulfides, HAMP protein, human, Hepcidins | IM | 22705624, S0196-9781(12)00262-8, 10.1016/j.peptides.2012.06.001 | Hepcidin was first identified as an antimicrobial peptide present in human serum and urine. It was later demonstrated that hepcidin is the long sought hormone that regulates iron homeostasis in mammals. The native peptide of 25 amino acids (Hepc25) contains four disulfide bridges that maintain a β-hairpin motif. The aim of the present study was to assess whether the intramolecular disulfide bridges are necessary for Hepc25 antimicrobial activity. We show that a synthetic peptide corresponding to human Hepc25, and which contains the four disulfide bridges, has an antibacterial activity against several strains of Gram-positive and Gram-negative bacteria. On the contrary, a synthetic peptide where all cysteines were replaced by alanines (Hepc25-Ala) had no detectable activity against the same strains of bacteria. In a further step, the mode of action of Hepc25 on Escherichia coli was studied. SYTOX Green uptake was used to assess bacterial membrane integrity. No permeabilization of the membrane was observed with Hepc25, indicating that this peptide does not kill bacteria by destroying their membranes. Gel retardation assay showed that the Hepc25 binds to DNA with high efficiency, and that this binding ability is dependent on the presence of the intramolecular disulfide bridges. Reduction of Hepc25 or replacement of the eight cysteines by alanine residues led to peptides that were no longer able to bind DNA in the in vitro assay. Altogether, these results demonstrate that Hepc25 should adopt a three-dimensional structure stabilized by the intramolecular disulfide bridges in order to have antibacterial activity. | Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Disulfides, Electrophoretic Mobility Shift Assay, Escherichia coli, Gram-Negative Bacteria, Gram-Positive Bacteria, Hepcidins, Humans, Structure-Activity Relationship | null |
22,705,612 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Pattern of patch test reactivity among patients with clinical diagnosis of contact dermatitis: a hospital-based study. | Almogren Adel, Shakoor Zahid, GadEl Rab Mohammad Osman, Adam Mustafa Hussein | eng | null | Journal Article | Allergens, nickel sulfate, Nickel | IM | 22705612, 12-404, 10.5144/0256-4947.2012.404, PMC6081020, 20565501, 20609142, 19470301, 20967194, 17273160, 21137633, 19688151, 16029338, 15246935, 11736749, 11811923, 15319150, 16310529, 8879920, 8565485, 20136878, 11966696, 1633708, 8400900, 1362844, 9417366, 2282794, 11331700, 21294394, 14749021, 10735956, 15128314, 16128752, 14738721 | Contact allergy is associated with a significant morbidity all over the world. This study was performed to investigate the pattern of sensitization by contact allergens in the local population. | Adult, Allergens, Dermatitis, Allergic Contact, Female, Hospitals, University, Humans, Male, Middle Aged, Nickel, Patch Tests, Retrospective Studies, Saudi Arabia, Young Adult | null |
22,705,614 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Current evidence in diagnosis and treatment of proliferative verrucous leukoplakia. | Kharma Mohamed Yasser, Tarakji Basal | eng | null | Journal Article, Review | null | IM | 22705614, 12-412, 10.5144/0256-4947.2012.412, PMC6081025, 12846783, 1864068, 17944749, 280847, 10369289, 12846784, 62332, 6537892, 11518355, 9708924, 11323946, 8060064, 19186968, 12798406, 3862042, 10645398, 3480564, 1742990, 9039227, 9269017, 17448134, 17174763, 10218036, 14561360, 11113818, 20173704, 3458148, 7616456, 3467893, 12764683, 12767875 | Proliferative verrucous leukoplakia is multifocal and progressive lesion with a high rate of malignant transformation. This short review highlights the diagnosis, traditional treatment and the current management of the disease. A MEDLINE search for the specific English word including proliferative verrucous leukoplakia in the last two years (2009-2010). This study indicates the current evidence for the diagnosis and the management of proliferative verrucous leukoplakia to help the specialist in this domain for early diagnosis of proliferative verrucous leukoplakia according to the new criteria of diagnosis to prevent the recurrence and any malignant transformation. | Disease Progression, Early Diagnosis, Humans, Leukoplakia, Oral, Secondary Prevention | null |
22,705,615 | 2012-11-07 | 2024-03-30 | 0975-4466 | Annals of Saudi medicine | Borderline mucinous cystic ovarian tumor with mural nodules (carcinosarcoma). | Chang Juei-Shan, Chua Ching-Chuang, Lee Chin-Cheng | eng | null | Case Reports, Journal Article, Review | Antineoplastic Agents | IM | 22705615, 12-415, 10.5144/0256-4947.2012.415, PMC6081021, 1709621, 19597831, 17903197, 1917278, 1646153, 7083135, 1656803, 498013, 2982704, 8112957, 15882184, 3429109, 3192749, 18300813, 498014, 12409723 | Carcinosarcoma mural nodules arising form a mucinous ovarian neoplasm is very rare and only two published cases have been reported. We report a case of a 29-year-old female patient who suffered from severe lower abdominal pain unrelated to menstruation for 1 year. She came to our (Shin-Kong Hospital) gynecology outpatient department in February 2010. The CT scan revealed a large cystic tumor, measuring approximately 36 cm in greatest dimension and at least 2 solid foci were noted. The patient underwent left salpingo-oophorectomy. A carcinosarcoma mural nodule arising within a mucinous ovarian neoplasm was diagnosed. The patient was treated by further chemotherapy and was free of the disease at time of publication. We reviewed the published studies, and in particular looked at the histology and immunohistochemistry of tumors, in which sarcomatoid carcinoma and carcinosarcoma like nodules were diagnosed. We also discussed the differential diagnosis of the mural nodule in a mucinous cystic neoplasm. | Abdominal Pain, Adult, Antineoplastic Agents, Carcinosarcoma, Combined Modality Therapy, Diagnosis, Differential, Female, Humans, Neoplasms, Cystic, Mucinous, and Serous, Ovarian Neoplasms, Ovariectomy, Salpingectomy | null |
22,705,619 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Lethal Legionella infection in an immunocompromised child: first reported case in the Middle East. | Taha Sami A, Al-Ayed Tareq M, Al-Haider Sami A, Frayha Husn H | eng | null | Case Reports, Journal Article | Antigens, Bacterial | IM | 22705619, 12-430, 10.5144/0256-4947.2012.430, PMC6081022, 18419436, 14712368, 7800428, 335244, 8328493, 9643390, 1139040, 8879767, 2604513, 11864350, 12668578, 1424896, 3060246, 12089674 | Legionnaires disease continues to be underreported in the Middle East--a reflection of underdiagnosis, both clinically and by laboratory investigations. We draw the attention to this unusual cause of occasionally fatal, yet severe, pneumonia by reporting an immunocompromised infant who succumbed to Legionella pneumophila pneumonia. The urinary test for Legionella antigen was positive, and this was then confirmed by a bronchoalveolar fluid culture. Moreover we have reviewed the incidence, pathophysiology, association with immunodeficiency, diagnostic tools, and treatment in this case report. | Antigens, Bacterial, Bronchoalveolar Lavage Fluid, Fatal Outcome, Female, Humans, Immunocompromised Host, Infant, Legionella pneumophila, Legionnaires' Disease, Pneumonia, Bacterial, Saudi Arabia, Severity of Illness Index | null |
22,705,621 | 2012-11-07 | 2022-04-19 | 0975-4466 | Annals of Saudi medicine | Ruptured intracranial dermoid cyst. | Nittala Pramad Philip, Thomas Vinay V | eng | null | Case Reports, Journal Article | null | IM | 22705621, 12-438, 10.5144/0256-4947.2012.438, PMC6081015, 17507446 | null | Adult, Brain Neoplasms, Dermoid Cyst, Female, Headache, Humans, Magnetic Resonance Imaging, Rupture, Spontaneous, Severity of Illness Index | null |
22,705,625 | 2013-01-07 | 2012-08-24 | 1873-5169 | Peptides | Tremendous intron length differences of the BmKBT and a novel BmKBT-like peptide genes provide a mechanical basis for the rapid or constitutive expression of the peptides. | Nie Yao, Zeng Xian-Chun, Luo Xuesong, Wu Shifen, Zhang Lei, Cao Hanjun, Zhou Jianping, Zhou Lingli | eng | null | Journal Article, Research Support, Non-U.S. Gov't | KBT toxin, Buthus martensii, Scorpion Venoms | IM | 22705625, S0196-9781(12)00263-X, 10.1016/j.peptides.2012.06.002 | The cDNA sequence encoding a novel BmKBT-like peptide (referred to as BmKBy) was cloned and sequenced from the scorpion Mesobuthus martensii Karsch. Functional analysis indicated that both BmKBT and BmKBy possess strong toxicity in mice, but very weak toxicity in cotton bollworm. Phylogenetic analysis showed that BmKBy and BmKBT represent evolutionary intermediates between the α- and β-toxins from scorpions. The genomic sequences of BmKBT and BmKBy were also obtained. It is interesting to see that two genes, which contain an intron of 225 and 1529bp, respectively, exactly code for the BmKBT peptide. One gene, which contains an intron of 1312bp, codes for BmKBy. Given that genes with long introns favor constitutive expression, whereas those with short introns are rapidly regulated in response to stimulations, the BmKBT_a and BmKBT_b genes provide a mechanical basis for either constitutive expression or rapid generation of the toxic peptides in response to different signals. | Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Introns, Larva, Lethal Dose 50, Mice, Molecular Sequence Data, Moths, Phylogeography, Protein Biosynthesis, Scorpion Venoms, Scorpions, Sequence Analysis, DNA, Sequence Homology, Amino Acid | null |
22,705,622 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Re: Glycogen hepatopathy in a 13-year-old male with type 1 diabetes. | Al-Mendalawi Mahmood | eng | null | Letter, Comment | Liver Glycogen | IM | 22705622, 12-437a, 10.5144/0256-4947.2012.437a, PMC6081018, 14635736, 15223230, 21727748 | null | Diabetes Mellitus, Type 1, Hepatomegaly, Humans, Liver Glycogen, Male | null |
22,705,627 | 2012-10-25 | 2012-06-20 | 1361-6560 | Physics in medicine and biology | Tumour control probability derived from dose distribution in homogeneous and heterogeneous models: assuming similar pharmacokinetics, (125)Sn-(177)Lu is superior to (90)Y-(177)Lu in peptide receptor radiotherapy. | Walrand Stephan, Hanin François-Xavier, Pauwels Stanislas, Jamar François | eng | null | Journal Article | Radioisotopes | IM | 22705627, 10.1088/0031-9155/57/13/4263 | Clinical trials on (177)Lu-(90)Y therapy used empirical activity ratios. Radionuclides (RN) with larger beta maximal range could favourably replace (90)Y. Our aim is to provide RN dose-deposition kernels and to compare the tumour control probability (TCP) of RN combinations. Dose kernels were derived by integration of the mono-energetic beta-ray dose distributions (computed using Monte Carlo) weighted by their respective beta spectrum. Nine homogeneous spherical tumours (1-25 mm in diameter) and four spherical tumours including a lattice of cold, but alive, spheres (1, 3, 5, 7 mm in diameter) were modelled. The TCP for (93)Y, (90)Y and (125)Sn in combination with (177)Lu in variable proportions (that kept constant the renal cortex biological effective dose) were derived by 3D dose kernel convolution. For a mean tumour-absorbed dose of 180 Gy, 2 mm homogeneous tumours and tumours including 3 mm diameter cold alive spheres were both well controlled (TCP > 0.9) using a 75-25% combination of (177)Lu and (90)Y activity. However, (125)Sn-(177)Lu achieved a significantly better result by controlling 1 mm-homogeneous tumour simultaneously with tumours including 5 mm diameter cold alive spheres. Clinical trials using RN combinations should use RN proportions tuned to the patient dosimetry. (125)Sn production and its coupling to somatostatin analogue appear feasible. Assuming similar pharmacokinetics (125)Sn is the best RN for combination with (177)Lu in peptide receptor radiotherapy justifying pharmacokinetics studies in rodent of (125)Sn-labelled somatostatin analogues. | Humans, Models, Biological, Neoplasms, Probability, Radiation Dosage, Radioisotopes, Radiotherapy Dosage, Treatment Outcome | null |
22,705,626 | 2012-11-21 | 2016-11-25 | 1473-558X | Neuroreport | Rapid recovery of tissue hypoxia by cotransplantation of endothelial cells. | Oh Jinsoo, Kim Keung Nyun, Yoon Do Heum, Han Seong Rok, Shin Dong Ah, Ha Yoon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705626, 10.1097/WNR.0b013e3283555d1b | Disruption of blood vessels caused by a spinal cord injury leads to tissue hypoxia. This hypoxic condition reduces the survival of transplanted stem cells, consequentially decreasing the effectiveness of stem cell therapy. In this study, we investigated the correlation between angiogenesis and the survival of transplanted neural stem cells in a spinal cord injury model. Hypoxia-specific luciferase-expressing neural stem cells (EpoSV-Luc NSC) were used as a tool for the detection of hypoxia caused by a spinal cord injury. In vivo, angiogenesis by cotransplantation of endothelial cells quickly recovered tissue hypoxia caused by a spinal cord injury. As a result, cotransplantation of endothelial cells improved the survival of neural stem cells transplanted into the injured spinal cord. | Animals, Human Umbilical Vein Endothelial Cells, Hypoxia, Neovascularization, Physiologic, Neural Stem Cells, Rats, Spinal Cord Injuries, Stem Cell Transplantation | null |
22,705,620 | 2012-11-07 | 2021-10-21 | 0975-4466 | Annals of Saudi medicine | Life-saving percutaneous intervention in young patient with massive pulmonary embolism. | Yaakob Zul Hilmi, Undok Abdul Wahab, Abidin Imran Zainal, Wan Ahmad Wan Azman | eng | null | Case Reports, Journal Article | null | IM | 22705620, 12-433, 10.5144/0256-4947.2012.433, PMC6081019, 16264048, 18198243, 18757870 | Massive pulmonary embolism (PE) is not an uncommon condition, and it usually carries a high risk of mortality. It is one of the fatal conditions that commonly affect young patients. A definitive treatment for patients with massive PE is still lacking, and surgical intervention carries a substantial mortality risk. Thus, percutaneous intervention (clot fragmentation and/or aspiration) remains an option in some patients, specifically in those with a risk of bleeding, contraindicating the use of thrombolysis. There have been no randomized trials to validate percutaneous intervention in massive PE. A sufficient level of evidence is still lacking, and its use depends upon the expert committee's opinion and study of previous case reports. We present a 23-year-old man with first onset massive PE secondary to protein C deficiency, who was treated successfully with the combination of systemic thrombolysis and percutaneous interventions. | Angiography, Catheterization, Combined Modality Therapy, Humans, Male, Protein C Deficiency, Pulmonary Embolism, Thrombolytic Therapy, Young Adult | null |
22,705,628 | 2013-03-21 | 2014-11-20 | 1872-9711 | Neurotoxicology | Exposure of young rats to diphenyl ditelluride during lactation affects the homeostasis of the cytoskeleton in neural cells from striatum and cerebellum. | Heimfarth Luana, Reis Karina Pires, Loureiro Samanta Oliveira, de Lima Bárbara Ortiz, da Rocha João Batista Teixeira, Pessoa-Pureur Regina | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Benzene Derivatives, Glial Fibrillary Acidic Protein, Neurofilament Proteins, Organometallic Compounds, Phosphates, Phosphorus Isotopes, diphenylditelluride | IM | 22705628, S0161-813X(12)00135-0, 10.1016/j.neuro.2012.06.003 | In the present report we examined the effect of maternal exposure to diphenyl ditelluride (PhTe)(2) (0.01 mg/kg body weight) during the first 14 days of lactational period on the activity of some protein kinases targeting the cytoskeleton of striatum and cerebellum of their offspring. We analyzed the phosphorylating system associated with glial fibrillary acidic protein (GFAP), and neurofilament of low, medium and high molecular weight (NF-L, NF-M and NF-H, respectively) of pups on PND 15, 21, 30 and 45. We found that (PhTe)(2) induced hyperphosphorylation of all the proteins studied on PND 15 and 21, recovering control values on PND 30 and 45. The immunocontent of GFAP, NF-L, NF-M and NF-H in the cerebellum of 15-day-old pups was increased. Western blot assays showed activation/phosphorylation of Erk1/2 on PND 21 and activation/phosphorylation of JNK on PND 15. Otherwise, p38MAPK was not activated in the striatum of (PhTe)(2) exposed pups. On the other hand, the cerebellum of pups exposed to (PhTe)(2) presented activated/phosphorylated Erk1/2 on PND 15 and 21 as well as activated/phosphorylated p38MAPK on PND 21, while JNK was not activated. Western blot assays showed that both in the striatum and in the cerebellum of (PhTe)(2) exposed pups, the immunocontent of the catalytic subunit of PKA (PKAcα) was increased on PND 15. Western blot showed that the phosphorylation level of NF-L Ser55 and NF-M/NF-H KSP repeats was increased in the striatum and cerebellum of both 15- and 21-day-old pups exposed to (PhTe)(2). Diphenyl diselenide (PhSe)(2), the selenium analog of (PhTe)(2), prevented (PhTe)(2)-induced hyperphosphorylation of striatal intermediate filament (IF) proteins but it failed to prevent the action of (PhTe)(2) in cerebellum. Western blot assay showed that the (PhSe)(2) prevented activation/phosphorylation of Erk1/2, JNK and PKAcα but did not prevent the stimulatory effect of (PhTe)(2) on p38MAPK in cerebellum at PND 21. In conclusion, this study demonstrated that dam exposure to low doses of (PhTe)(2) can alter cellular signaling targeting the cytoskeleton of striatum and cerebellum in the offspring in a spatiotemporal manner, which can be related to the neurotoxic effects of (PhTe)(2). | Age Factors, Analysis of Variance, Animals, Animals, Newborn, Benzene Derivatives, Body Weight, Cerebellum, Corpus Striatum, Cytoskeleton, Female, Gene Expression Regulation, Developmental, Glial Fibrillary Acidic Protein, Homeostasis, In Vitro Techniques, Neurofilament Proteins, Neurons, Organometallic Compounds, Phosphates, Phosphorus Isotopes, Pregnancy, Prenatal Exposure Delayed Effects, Rats, Rats, Wistar, Signal Transduction, Time Factors | null |
22,705,629 | 2012-10-18 | 2012-06-25 | 1873-6297 | Acta psychologica | Differentiating the differential rotation effect. | Boyarskaya Evgenia, Hecht Heiko | eng | null | Journal Article | null | IM | 22705629, S0001-6918(12)00079-0, 10.1016/j.actpsy.2012.05.008 | As an observer views a picture from different viewing angles, objects in the picture appear to maintain their orientation relative to the observer. For instance, the eyes of a portrait appear to follow the observer as he or she views the image from different angles. We have explored this rotation effect, often called the Mona Lisa effect. We report three experiments that used portrait photographs to test variations of the Mona Lisa effect. The first experiment introduced picture displacements relative to the observer in directions beyond the horizontal plane. The Mona Lisa effect remained robust for vertical and/or diagonal observer displacements. The experiment also included conditions in which the portrait had averted gaze directions. An interaction between picture position relative to the observer and gaze direction was found. The second experiment followed up on very pronounced individual differences, suggesting that the Mona Lisa effect is even stronger than it should be for half of all observers (over-rotators). These individual differences do not correlate with any of the standard personality dimensions (Big Five) or with spatial intelligence. In the third experiment, we extended the experiment to virtual 3D heads using the same gaze directions and picture displacements as for the 2D portrait faces. Besides the picture displacements relative to the observer, we also added observer displacements relative to the picture. 3D pictures showed the Mona Lisa effect, but to a smaller extent than did 2D pictures. | Adult, Female, Humans, Individuality, Male, Personality, Rotation, Space Perception, Visual Perception | null |
22,705,630 | 2012-10-18 | 2019-12-10 | 1873-6297 | Acta psychologica | Offline improvement occurs for temporal stability but not accuracy following practice of integer and non-integer rhythms. | Wright David L, Rhee Joohyun, Blischke Klaus, Erlacher Daniel, Brueckner Sebastian | eng | null | Journal Article | null | IM | 22705630, S0001-6918(12)00081-9, 10.1016/j.actpsy.2012.05.010 | Procedural learning benefits from memory processes occurring outside practice resulting in offline learning. Offline gains have been demonstrated almost exclusively for the ordinal structure of sequential motor tasks. Many skills also demand that the correct serial order of events be appropriately timed. Evidence indicates that the temporal aspect of a procedural skill can be encoded independent of serial order knowledge and governed by at least two distinct neural circuits. The present experiment determined if (a) offline gains emerge for temporal learning, and (b) if such gains occur for timing supervised by distinct timing systems. Participants experienced 216 practice trials of a 7-key press sequence that involved integer- or non-integer timing rhythms. Twenty-four hours after training 30 test trials were administered. Results revealed robust offline enhancement for timing performance of the non-integer based temporal sequences. This improvement was localized to stabilization of the required relative but not absolute time profiles. The neural circuitry central to supporting the performance of non-integer timing sequences is also a principal constituent of what is described as the "cognitive" timing system. Timing governed by this system appears most susceptible to offline gains via consolidation. | Adult, Humans, Learning, Motor Skills, Practice, Psychological, Time Factors | null |
22,705,631 | 2012-10-18 | 2019-12-10 | 1873-6297 | Acta psychologica | Distinct modes of executing movement sequences: reacting, associating, and chunking. | Verwey Willem B, Abrahamse Elger L | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705631, S0001-6918(12)00078-9, 10.1016/j.actpsy.2012.05.007 | Responding to individual key-specific stimuli in entirely unfamiliar keying sequences is said to involve a reaction mode. With practice, short keying sequences can be executed in the chunking mode. This is indicated by the first stimulus sufficing for rapid execution of the entire sequence. The present study explored whether an associative mode develops also in participants who practice short keying sequences. This associative mode would involve priming by earlier events of responses to external stimuli, and is believed to be responsible for skill in the Serial Reaction Time task. In the present study participants practiced two discrete 6-key sequences. In the ensuing test phase, participants were prevented from using the chunking mode by including two deviant stimuli in most sequences. The results from the remaining - unchanged - familiar sequences confirmed that participants no longer used the chunking mode, but as predicted by associative learning these sequences were executed faster than unfamiliar sequences. | Adolescent, Adult, Female, Humans, Male, Motor Skills, Practice, Psychological, Reaction Time, Repetition Priming, Serial Learning | null |
22,705,632 | 2012-10-26 | 2012-06-26 | 1364-548X | Chemical communications (Cambridge, England) | Efficient synthesis of optically active α-quaternary amino acids by highly diastereoselective [2,3]-rearrangement of allylic ammonium ylides. | Zhu Ting-Shun, Xu Ming-Hua | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Amino Acids, Quaternary Ammonium Compounds, Solvents | IM | 22705632, 10.1039/c2cc33027j | A pincer-like chiral auxiliary strategy for synthesizing various optically active α,α-disubstituted amino acids in high yields with excellent enantioselectivities is described. | Amino Acids, Quaternary Ammonium Compounds, Solvents, Stereoisomerism, Temperature | null |
22,705,633 | 2013-01-07 | 2013-11-21 | 1878-7568 | Acta biomaterialia | Impact of plasma chemistry versus titanium surface topography on osteoblast orientation. | Rebl Henrike, Finke Birgit, Lange Regina, Weltmann Klaus-Dieter, Nebe J Barbara | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Coated Materials, Biocompatible, Plasma Gases, Water, Allylamine, Titanium | IM | 22705633, S1742-7061(12)00263-2, 10.1016/j.actbio.2012.06.015 | Topographical and chemical modifications of biomaterial surfaces both influence tissue physiology, but unfortunately little knowledge exists as to their combined effect. There are many indications that rough surfaces positively influence osteoblast behavior. Having determined previously that a positively charged, smooth titanium surface boosts osteoblast adhesion, we wanted to investigate the combined effects of topography and chemistry and elucidate which of these properties is dominant. Polished, machined and corundum-blasted titanium of increasing microroughness was additionally coated with plasma-polymerized allylamine (PPAAm). Collagen I was then immobilized using polyethylene glycol diacid and glutar dialdehyde. On all PPAAm-modified surfaces (i) adhesion of human MG-63 osteoblastic cells increased significantly in combination with roughness, (ii) cells resemble the underlying structure and melt with the surface, and (iii) cells overcome the restrictions of a grooved surface and spread out over a large area as indicated by actin staining. Interestingly, the cellular effects of the plasma-chemical surface modification are predominant over surface topography, especially in the initial phase. Collagen I, although it is the gold standard, does not improve surface adhesion features comparably. | Actin Cytoskeleton, Allylamine, Cell Adhesion, Cell Line, Cell Membrane, Cell Nucleolus, Cell Shape, Coated Materials, Biocompatible, Humans, Microscopy, Electron, Scanning, Osteoblasts, Plasma Gases, Polymerization, Surface Properties, Titanium, Water | null |
22,705,634 | 2012-12-07 | 2022-03-18 | 1878-7568 | Acta biomaterialia | Collagen for bone tissue regeneration. | Ferreira Ana Marina, Gentile Piergiorgio, Chiono Valeria, Ciardelli Gianluca | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Biocompatible Materials, Collagen | IM | 22705634, S1742-7061(12)00262-0, 10.1016/j.actbio.2012.06.014 | In the last decades, increased knowledge about the organization, structure and properties of collagen (particularly concerning interactions between cells and collagen-based materials) has inspired scientists and engineers to design innovative collagen-based biomaterials and to develop novel tissue-engineering products. The design of resorbable collagen-based medical implants requires understanding the tissue/organ anatomy and biological function as well as the role of collagen's physicochemical properties and structure in tissue/organ regeneration. Bone is a complex tissue that plays a critical role in diverse metabolic processes mediated by calcium delivery as well as in hematopoiesis whilst maintaining skeleton strength. A wide variety of collagen-based scaffolds have been proposed for different tissue engineering applications. These scaffolds are designed to promote a biological response, such as cell interaction, and to work as artificial biomimetic extracellular matrices that guide tissue regeneration. This paper critically reviews the current understanding of the complex hierarchical structure and properties of native collagen molecules, and describes the scientific challenge of manufacturing collagen-based materials with suitable properties and shapes for specific biomedical applications, with special emphasis on bone tissue engineering. The analysis of the state of the art in the field reveals the presence of innovative techniques for scaffold and material manufacturing that are currently opening the way to the preparation of biomimetic substrates that modulate cell interaction for improved substitution, restoration, retention or enhancement of bone tissue function. | Biocompatible Materials, Bone Regeneration, Collagen, Microscopy, Electron, Scanning, Protein Conformation, Tissue Engineering | null |
22,705,635 | 2013-01-07 | 2012-08-28 | 1878-7568 | Acta biomaterialia | Comparison of properties between NIPAAm-based simultaneously physically and chemically gelling polymer systems for use in vivo. | Bearat Hanin H, Lee Bae Hoon, Vernon Brent L | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Acrylamides, Cross-Linking Reagents, Gels, Polymers, poly(N-isopropylacrylamide-co-hydroxyethyl methacrylate-acrylate), Polyhydroxyethyl Methacrylate | IM | 22705635, S1742-7061(12)00260-7, 10.1016/j.actbio.2012.06.012 | In this work, a comparison between two different physical-chemical gels, poly(NIPAAm-co-cysteamine) with poly(NIPAAm-co-cysteamine-vinylsulfone) and poly(NIPAAm-co-cysteamine) with poly(NIPAAm-co-HEMA-acrylate), is made. These hydrogels undergo gelation via dual mechanisms: temperature sensitivity (physical gelation) and chemical crosslinking (chemical gelation). The advantages of using both gelation mechanisms are to reduce the creep experienced by purely physical gels and to increase the elastic modulus of purely chemical gels. Here, the physical-chemical gels were synthesized and characterized for their chemical, structural, thermal, mechanical and morphological properties. The gels were also tested for their gelation kinetics, swelling, degradation and cytotoxicity. The copolymers were successfully synthesized and their phase transition temperatures fall in a feasible range (29-34°C) for use in vivo. With rheology, it was shown that use of simultaneous physical and chemical gelation resulted in improved properties, with increased elastic moduli and reduced frequency dependence. The rates of reaction of thiols to vinyls differ between the two systems, demonstrating a greater effect of chemical gelation in one gelling system over the other, due to the faster rate of thiols consumed into reaction. The morphology of the gels proved to be quite different when analyzed by scanning electron microscopy, showing differences in swelling behaviors. Cell studies illustrated good growth of cells exposed to the gels. Both hydrogels, although possessing slight differences, demonstrate the capability of being injected in vivo for use as embolic agents for occlusion of aneurysms. | 3T3 Cells, Acrylamides, Animals, Calorimetry, Differential Scanning, Cell Death, Cell Survival, Cross-Linking Reagents, Gels, Kinetics, Magnetic Resonance Spectroscopy, Materials Testing, Mice, Microscopy, Electron, Scanning, Molecular Weight, Polyhydroxyethyl Methacrylate, Polymers, Rheology, Spectroscopy, Fourier Transform Infrared, Temperature, Time Factors | null |
22,705,636 | 2013-01-07 | 2016-11-25 | 1878-7568 | Acta biomaterialia | A crimp-like microarchitecture improves tissue production in fibrous ligament scaffolds in response to mechanical stimuli. | Surrao Denver C, Fan Jackie C Y, Waldman Stephen D, Amsden Brian G | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Collagen Type I, Collagen Type III, Polyesters, poly(lactide) | IM | 22705636, S1742-7061(12)00264-4, 10.1016/j.actbio.2012.06.016 | The aim of this study was to determine the influence of a crimp-like microarchitecture within electrospun polymer scaffolds on fibroblast extracellular matrix (ECM) production when cultured under dynamic conditions. Electrospun poly(L-lactide-co-D,L-lactide) scaffolds possessing a wave pattern similar to collagen crimp (amplitude: 5 μm and wavelength: 46 μm) were seeded with bovine fibroblasts and mechanically stimulated under dynamic uniaxial tension. The effect of strain amplitude (5%, 10% and 20%) was investigated in a short-term stimulation study. The 10% strain amplitude in the stimulated crimp-like fibre scaffold increased only collagen synthesis, while the 20% strain amplitude increased both collagen and sulphated proteoglycan synthesis compared to stimulated uncrimped (straight) fibre scaffolds and unloaded controls (crimp-like static fibre scaffolds). Alternatively, mechanical stimulation of fibroblasts seeded on uncrimped fibre scaffolds induced significant fibroblast proliferation compared to the stimulated crimp-like fibre scaffolds and no-load controls. Long-term, dynamic mechanical stimulation of fibroblasts seeded on crimp-like fibre scaffolds at 10% strain amplitude resulted in significantly up-regulated collagen accumulation and down-regulated sulphated proteoglycan accumulation. Additionally, the fibroblasts seeded on dynamically stimulated crimp-like fibre scaffolds appeared to form bundles that resembled fascicles, a characteristic hierarchical feature of the native ligament. Our findings demonstrate that fibroblasts seeded on crimp-like fibrous scaffolds respond more favourably (increased ECM synthesis and fascicle formation) to dynamic mechanical loading compared to those grown on scaffolds containing uncrimped (straight) fibres. | Animals, Cattle, Cell Proliferation, Collagen Type I, Collagen Type III, Extracellular Matrix, Fibroblasts, Immunohistochemistry, Ligaments, Microscopy, Electron, Scanning, Polyesters, Stress, Mechanical, Tissue Engineering, Tissue Scaffolds | null |
22,705,637 | 2013-04-03 | 2018-12-02 | 1878-7568 | Acta biomaterialia | Transfection of macrophages by collagen hollow spheres loaded with polyplexes: a step towards modulating inflammation. | Helary Christophe, Browne Shane, Mathew Asha, Wang Wenxin, Pandit Abhay | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Methacrylates, Nylons, Polymers, poly(2-(dimethylamino)ethyl methacrylate), Polyethylene Glycols, Collagen, DNA | IM | 22705637, S1742-7061(12)00265-6, 10.1016/j.actbio.2012.06.017 | Macrophages are key orchestrators of inflammation as they secrete proteases and inflammatory cytokines. To date, therapies aimed at modulating macrophage phenotype have failed due to the short half-life of biomolecules in the body. Therefore, inhibition of inflammation by gene therapy constitutes a new hope. In the present study, we have assessed collagen hollow spheres as a reservoir system for polyplexes in order to transfect human macrophages while preserving cell viability. Polyplexes were formed by complexing G-Luc plasmid with a poly(2-dimethylaminoethyl methacrylate) poly(ethylene glycol) based hyperbranched polymer. Several ratios of polymer/pDNA (5:1, 8:1, 10:1w/w) complexes in two different sphere sizes (1.24 and 4.5μm) were tested. Collagen hollow spheres were loaded with polyplexes up to 80μg of pDNA per mg of microspheres. The release of polyplexes from the spheres was delayed and prolonged i.e. 20% of the initial amount released in 5days. Following incubation with polyplex-loaded microspheres, macrophages were transfected (polyplex pDNA:polymer ratio 1:10w/w). In addition, collagen hollow spheres maintained cell viability as more than 80% of cells were viable after 4days in culture. In contrast, when used alone, polyplexes were seen to be toxic, while there was no transfection detected. Taken together, these results show that collagen hollow spheres may be used as a reservoir for controlled gene delivery to macrophages. Unlike existing gene delivery systems, this system allows for macrophage transfection with minimal toxicity. Hence, this system has a potential for the delivery of a therapeutic gene in order to modulate inflammation. | Cell Line, Tumor, Collagen, DNA, Humans, Inflammation, Macrophages, Methacrylates, Microspheres, Nylons, Plasmids, Polyethylene Glycols, Polymers, Transfection | null |
22,705,638 | 2012-10-24 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | The oxidized phospholipid PazePC modulates interactions between Bax and mitochondrial membranes. | Wallgren Marcus, Lidman Martin, Pham Quoc Dat, Cyprych Konrad, Gröbner Gerhard | eng | null | Journal Article, Research Support, Non-U.S. Gov't | 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine, Recombinant Proteins, bcl-2-Associated X Protein, Phosphorylcholine | IM | 22705638, S0005-2736(12)00193-9, 10.1016/j.bbamem.2012.06.005 | Activation of the pro-apoptotic protein Bax under intracellular oxidative stress is closely related to its association with the mitochondrial outer membrane (MOM) system, ultimately resulting in cell death. The precise mechanism by which this activation and the subsequent structural changes in the protein occur is currently unknown. In addition to triggering the onset of apoptosis, oxidative stress generates oxidized lipids whose impact on mitochondrial membrane integrity and the activity of membrane-associated Bax is unclear. We therefore devised a model system that mimics oxidative stress conditions by incorporating oxidized phospholipids (OxPls) into mitochondria-like liposomes, and studied the OxPls' impact on Bax-membrane interactions. Differential scanning calorimetry (DSC) was used to study membrane organization and protein stability, while conformational changes in the protein upon contact with lipid vesicles were monitored using far-UV circular dichroism (CD) spectroscopy. The thermograms for liposomes containing the OxPl 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC) differed dramatically from those for unmodified liposomes. Moreover, Bax exhibited enhanced thermal stability in the presence of the modified liposomes, indicating that it interacted strongly with PazePC-containing membranes. The presence of PazePC also increased the α-helical character of Bax compared to the protein alone or with PazePC-free vesicles, at 10°C, 20°C, and 37°C. Presumably, the presence of PazePC-like OxPls a) increases the population of membrane-associated Bax and b) facilitates the protein's insertion into the membrane by distorting the bilayer's organization, as seen by solid-state high-resolution (1)H and (31)P magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. | Apoptosis, Calorimetry, Differential Scanning, Circular Dichroism, Electrophoresis, Polyacrylamide Gel, Humans, Intracellular Membranes, Magnetic Resonance Spectroscopy, Mitochondria, Oxidative Stress, Phosphorylcholine, Protein Binding, Recombinant Proteins, bcl-2-Associated X Protein | null |
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