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22,705,333 | 2013-03-22 | 2013-11-21 | 1950-6007 | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Thalidomide attenuates mammary cancer associated-inflammation, angiogenesis and tumor growth in mice. | Maria de Souza Cristina, Fonseca de Carvalho Luciana, da Silva Vieira Tamara, Cândida Araújo E Silva Ana, Teresa Paz Lopes Miriam, Alves Neves Diniz Ferreira Mônica, Passos Andrade Silvia, Dantas Cassali Geovanni | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Angiogenesis Inhibitors, Tumor Necrosis Factor-alpha, Vascular Endothelial Growth Factor A, Fibroblast Growth Factor 1, Thalidomide | IM | 22705333, S0753-3322(12)00054-6, 10.1016/j.biopha.2012.04.005 | Thalidomide has proven to exert anti-inflammatory, anti-proliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on key components (blood vessel formation, inflammatory cell recruitment/activation, cytokine production) of 4T1 mammary tumor in mice. In addition, tumor growth and lung metastasis were evaluated. 4T1 cells were injected subcutaneously into Balb/c mice. After tumor engraftment (5days), thalidomide (150mg/kg) was administered to the treated group for 7days. Tumors of control (saline) and treated groups were sized regularly, removed 12days after inoculation and processed for biochemical and immunohistological parameters to assess neovascularization, inflammation and proliferative activity. Daily oral dose of thalidomide was able to reduce in 46% the tumor volume. The number of metastasis in the lungs was less in the thalidomide-treated group compared with the control animals. Assessment of tumor vascularization revealed a significant decrease in blood vessels formation by thalidomide. Likewise, the expression of FGF-1 showed weaker cytoplasmic positivity in the group treated with thalidomide compared with the control group. The levels of two cytokines, VEGF (pro-angiogenic) and TNF-α (pro-inflammatory) were decreased in tumor samples of thalidomide-treated group compared with the control group. Accumulation of neutrophils or macrophages in the 4T1 tumor measured by the activities of inflammatory enzymes, myeloperoxidase (MPO) for neutrophils and N-acetyl-β-D-glucosaminidase (NAG) for macrophages was inhibited by the treatment. By targeting key components of 4T1 tumor simultaneously, thalidomide was effective in attenuating tumor growth and metastasis. This approach, suppression of inflammation and angiogenesis may provide further insights for both prevention and treatment of cancer. | Administration, Oral, Angiogenesis Inhibitors, Animals, Female, Fibroblast Growth Factor 1, Gene Expression Regulation, Neoplastic, Inflammation, Lung Neoplasms, Macrophages, Mammary Neoplasms, Experimental, Mice, Mice, Inbred BALB C, Neovascularization, Pathologic, Neutrophils, Thalidomide, Tumor Necrosis Factor-alpha, Vascular Endothelial Growth Factor A | null |
22,705,335 | 2013-01-04 | 2017-11-16 | 1950-6007 | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | The feature of Metabolic Syndrome in HIV naive patients is not the same of those treated: results from a prospective study. | Bonfanti Paolo, De Socio Giuseppe Vittorio, Ricci Elena, Antinori Andrea, Martinelli Canio, Vichi Francesca, Penco Giovanni, Madeddu Giordano, Orofino Giancarlo, Valsecchi Laura, Rusconi Stefano, Menzaghi Barbara, Pocaterra Daria, Quirino Tiziana | eng | null | Journal Article | Anti-HIV Agents, Blood Glucose, Cholesterol, HDL, Triglycerides | IM | 22705335, S0753-3322(12)00023-6, 10.1016/j.biopha.2012.01.005 | Metabolic Syndrome (MS) is a common disorder combining obesity, dyslipidemia, hypertension, and insulin resistance. Its prevalence among HIV-infected people is still debated. Besides, how antiretroviral therapy and HIV infection per se are related to MS is still unclear. All treatment-naïve patients attending scheduled visits at CISAI group hospitals between January and December 2007 were eligible for the study. Patients without MS at enrolment were followed-up for 3 years or until they developed MS, diagnosed according to the National Cholesterol Education Program (NCEP) definition. The main objective was to assess the 3-years incidence of MS. MS was evaluated for 188 subjects. Out of them, 62 (33.0%) had started HAART at enrolment, whereas 67 (35.6%) more started during the observation. 59 (31.4%) were still treatment-naive at the study end. MS was newly diagnosed in 14 patients. The incidence was 2.60 cases/100 person-years (95% CI 1.47-4.51), 2.75 (1.11-5.72) among HAART-naïve patients and 2.65 (1.23-5.03) in subjects on HAART. Blood pressure did not change in the study period, whereas in naive patients the HDL level significantly lowered (median -6.0 vs. 4.0, P<0.0001) compared to HAART-treated patients. Triglicerides increased significantly in HAART subjects (median 12.0 vs. 1.0, P=0.02), as well as blood glucose (median 6.0 vs. 1.0, P=0.01). In our population, the overall MS incidence was low and largely similar in patients who started HAART or remained naive. However, the feature of MS was different in the two groups, suggesting that in untreated and treated patients MS developed through different metabolic pathways. | Adult, Anti-HIV Agents, Antiretroviral Therapy, Highly Active, Blood Glucose, Blood Pressure, Cholesterol, HDL, Cohort Studies, Female, Follow-Up Studies, HIV Infections, Humans, Incidence, Male, Metabolic Syndrome, Middle Aged, Prospective Studies, Triglycerides | null |
22,705,337 | 2012-11-30 | 2021-10-21 | 1474-4465 | The Lancet. Neurology | Primary or secondary prevention for AD: who cares? | Frisoni Giovanni B, Trojanowski John Q | eng | P30 AG010124 (NIA NIH HHS, United States) | Letter, Comment | Antibodies, Monoclonal, Presenilins | IM | 22705337, S1474-4422(12)70143-6, 10.1016/S1474-4422(12)70143-6, PMC4533993, NIHMS713232, 22229117, 22710749, 17553989, 17293358, 21514248 | null | Alzheimer Disease, Antibodies, Monoclonal, Humans, Presenilins | null |
22,705,336 | 2012-10-19 | 2023-11-04 | 1096-0953 | Environmental research | Relationship between birth weight and exposure to airborne fine particulate potassium and titanium during gestation. | Bell Michelle L, Belanger Kathleen, Ebisu Keita, Gent Janneane F, Leaderer Brian P | eng | R01ES016317 (NIEHS NIH HHS, United States); R01 ES015028 (NIEHS NIH HHS, United States); R01 ES019560 (NIEHS NIH HHS, United States); R01ES015028 (NIEHS NIH HHS, United States); R01 ES019587 (NIEHS NIH HHS, United States); R01ES019587 (NIEHS NIH HHS, United States); R01 ES016317 (NIEHS NIH HHS, United States); R01ES019560 (NIEHS NIH HHS, United States) | Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | Air Pollutants, Particulate Matter, Titanium, Potassium | IM | 22705336, S0013-9351(12)00173-9, 10.1016/j.envres.2012.05.004, PMC3418490, NIHMS388427, 15471726, 17618747, 16805397, 19337525, 14644658, 7936899, 3880598, 12573907, 16466348, 24634604, 17637932, 17938740, 21306972, 284865, 20664652, 21981989, 17687714, 19590690, 18766434, 8559516, 18560536, 15068936, 11368231, 8342728, 22617723, 21193387, 19215915, 18417555, 21172759, 20064787, 21463978, 16450204, 22145497, 3417989, 16039875, 15629991, 17637911, 20811286, 19654929, 19496481, 20025755, 20392805, 17366813, 15811825, 19299499, 17803614, 8484367, 21835727, 23930137, 20123621 | Airborne particles are linked to numerous health impacts, including adverse pregnancy outcomes. Most studies of particles examined total mass, although the chemical structure of particles varies widely. We investigated whether mother's exposure to potassium (K) and titanium (Ti) components of airborne fine particulate matter (PM(2.5)) during pregnancy was associated with birth weight or risk of low birth weight (<2500 g) for term infants. The study population was 76,788 infants born in four counties in Connecticut and Massachusetts, US, for August 2000-February 2004. Both K and Ti were associated with birth weight. An interquartile range (IQR) increase K was associated with an 8.75% (95% confidence interval (CI): 1.24-16.8%) increase in risk of low birth weight. An IQR increase in Ti was associated with a 12.1% (95% CI: 3.55-21.4%) increase in risk of low birth weight, with an estimate of 6.41% (95% CI: -5.80-20.2%) for males and 16.4% (95% CI: 5.13-28.9%) for females. Results were robust to sensitivity analysis of first births only, but not adjustment by co-pollutants. Disentangling the effects of various chemical components is challenging because of the covariance among some components due to similar sources. Central effect estimates for infants of African-American mothers were higher than those of white mothers, although the confidence intervals overlapped. Our results indicate that exposure to airborne potassium and titanium during pregnancy is associated with lower birth weight. Associations may relate to chemical components of sources producing K and Ti. | Adult, Black or African American, Air Pollutants, Birth Weight, Connecticut, Female, Humans, Infant, Newborn, Linear Models, Male, Massachusetts, Particulate Matter, Potassium, Pregnancy, Prenatal Exposure Delayed Effects, Risk Assessment, Titanium, White People | null |
22,705,338 | 2012-11-29 | 2012-07-16 | 1879-3134 | Mathematical biosciences | A kinetic model of ERK cyclic pathway on substrate control. | Hirashima Tsuyoshi | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Extracellular Signal-Regulated MAP Kinases | IM | 22705338, S0025-5564(12)00127-7, 10.1016/j.mbs.2012.05.011 | Extracellular signal-regulated kinase (ERK) is a key factor in the widely used signaling cascade of phosphorylation-dephosphorylation cycles and plays pivotal roles in many aspects of biological processes. Experimental studies in yeast and in Drosophila embryo have suggested that the phosphorylation and spatial localization of ERK are influenced by the level of its downstream substrates. However, the mechanism, through which these substrates control properties of ERK signaling, has been unclear. I propose a mass-action kinetic model of ERK cycle with its substrate, and demonstrate that the substrate can modulate the ERK activity by directly interacting with ERK. The model shows that the addition of substrate controls the level of ERK phosphorylation positively or negatively, depending on the balance between dissociation constants of ERK-substrate interaction and properties of ERK cyclic signaling in the absence of the substrate. In addition, by considering cellular compartments, cytosol and nucleus, the substrate can lead to nuclear accumulation of ERK, suggesting that the substrate can act as a nuclear anchor of ERK. The model gives a possible mechanism that can account for substrate-mediated modulation of ERK signaling. | Animals, Computer Simulation, Drosophila, Extracellular Signal-Regulated MAP Kinases, Kinetics, MAP Kinase Signaling System, Models, Biological, Phosphorylation, Signal Transduction | null |
22,705,339 | 2013-01-07 | 2012-08-06 | 1879-3134 | Mathematical biosciences | Monitoring and prediction of an epidemic outbreak using syndromic observations. | Skvortsov Alex, Ristic Branko | eng | null | Journal Article | null | IM | 22705339, S0025-5564(12)00126-5, 10.1016/j.mbs.2012.05.010 | The paper presents a method for syndromic surveillance of an epidemic outbreak due to an emerging disease, formulated in the context of stochastic nonlinear filtering. The dynamics of the epidemic is modeled using a stochastic compartmental epidemiological model with inhomogeneous mixing. The syndromic (typically non-medical) observations of the number of infected people (e.g. visits to pharmacies, sale of certain products, absenteeism from work/study, etc.) are assumed available for monitoring and prediction of the epidemic. The state of the epidemic, including the number of infected people and the unknown parameters of the model, are estimated via a particle filter. The numerical results indicate that the proposed framework can provide useful early prediction of the epidemic peak if the uncertainty in prior knowledge of model parameters is not excessive. | Algorithms, Communicable Diseases, Data Collection, Disease Outbreaks, Epidemiologic Methods, Humans, Models, Statistical, Stochastic Processes | null |
22,705,341 | 2012-12-10 | 2012-08-03 | 1095-9947 | Fish & shellfish immunology | Edwardsiella tarda sialidase: pathogenicity involvement and vaccine potential. | Jin Ren-ping, Hu Yong-hua, Sun Bo-guang, Zhang Xiao-hua, Sun Li | eng | null | Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | Bacterial Vaccines, Fish Proteins, Recombinant Proteins, Vaccines, Subunit, Vaccines, Synthetic, Oxo-Acid-Lyases, N-acetylneuraminate lyase | IM | 22705341, S1050-4648(12)00205-7, 10.1016/j.fsi.2012.06.002 | Bacterial sialidases are a group of glycohydrolases that are known to play an important role in invasion of host cells and tissues. In this study, we examined in a model of Japanese flounder (Paralichthys olivaceus) the potential function of NanA, a sialidase from the fish pathogen Edwardsiella tarda. NanA is composed of 670 residues and shares low sequence identities with known bacterial sialidases. In silico analysis indicated that NanA possesses a sialidase domain and an autotransporter domain, the former containing five Asp-boxes, a RIP motif, and the conserved catalytic site of bacterial sialidases. Purified recombinant NanA (rNanA) corresponding to the sialidase domain exhibited glycohydrolase activity against sialic acid substrate in a manner that is pH and temperature dependent. Immunofluorescence microscopy showed binding of anti-rNanA antibodies to E. tarda, suggesting that NanA was localized on cell surface. Mutation of nanA caused drastic attenuation in the ability of E. tarda to disseminate into and colonize fish tissues and to induce mortality in infected fish. Likewise, cellular study showed that the nanA mutant was significantly impaired in the infectivity against cultured flounder cells. Immunoprotective analysis showed that rNanA in the form of a subunit vaccine conferred effective protection upon flounder against lethal E. tarda challenge. rNanA vaccination induced the production of specific serum antibodies, which enhanced complement-mediated bactericidal activity and reduced infection of E. tarda into flounder cells. Together these results indicate that NanA plays an important role in the pathogenesis of E. tarda and may be exploited for the control of E. tarda infection in aquaculture. | Animals, Bacterial Vaccines, Cell Culture Techniques, Edwardsiella tarda, Enterobacteriaceae Infections, Enzyme-Linked Immunosorbent Assay, Fish Diseases, Fish Proteins, Flounder, Injections, Intraperitoneal, Microscopy, Fluorescence, Molecular Sequence Data, Oxo-Acid-Lyases, Phylogeny, Recombinant Proteins, Sequence Alignment, Sequence Analysis, Protein, Vaccines, Subunit, Vaccines, Synthetic, Virulence | null |
22,705,340 | 2013-07-02 | 2021-10-21 | 1873-7064 | Neuropharmacology | Potentiation of mGlu5 receptors with the novel enhancer, VU0360172, reduces spontaneous absence seizures in WAG/Rij rats. | D'Amore V, Santolini I, van Rijn C M, Biagioni F, Molinaro G, Prete A, Conn P J, Lindsley C W, Zhou Y, Vinson P N, Rodriguez A L, Jones C K, Stauffer S R, Nicoletti F, van Luijtelaar G, Ngomba R T | eng | R01 MH062646 (NIMH NIH HHS, United States); R01 NS031373 (NINDS NIH HHS, United States) | Journal Article | 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, N-cyclobutyl-6-((3-fluorophenyl)ethynyl)nicotinamide, Phosphatidylinositol Phosphates, Pyridines, Receptor, Metabotropic Glutamate 5, Receptors, Metabotropic Glutamate, Thiazoles, Niacinamide | IM | 22705340, S0028-3908(12)00252-3, 10.1016/j.neuropharm.2012.05.044, PMC3787880, NIHMS514010, 16725200, 14960010, 7588592, 21146957, 11008175, 11181420, 2122483, 12565928, 10328888, 2869493, 19112153, 9429128, 14574120, 17643266, 19864572, 12786977, 12438526, 21036182, 18022649, 15950993, 16302873, 2169562, 16487682, 18242586, 20055706, 17409230, 11487615, 19052194, 16005030, 11591458, 14586030, 10934271, 10530818, 1630600, 3095713, 11050106, 11850474, 21569017, 9619499, 7636025, 3143564, 12684257, 16513683, 10433269, 15767501, 20923853, 20132294, 10490445, 7084360, 19836978, 10854901, 10751563, 9144665, 10784111, 1743184, 21993209, 16051747, 19244358, 21277877, 3143552, 19276326, 11734603, 1650272 | Absence epilepsy is generated by the cortico-thalamo-cortical network, which undergoes a finely tuned regulation by metabotropic glutamate (mGlu) receptors. We have shown previously that potentiation of mGlu1 receptors reduces spontaneous occurring spike and wave discharges (SWDs) in the WAG/Rij rat model of absence epilepsy, whereas activation of mGlu2/3 and mGlu4 receptors produces the opposite effect. Here, we have extended the study to mGlu5 receptors, which are known to be highly expressed within the cortico-thalamo-cortical network. We used presymptomatic and symptomatic WAG/Rij rats and aged-matched ACI rats. WAG/Rij rats showed a reduction in the mGlu5 receptor protein levels and in the mGlu5-receptor mediated stimulation of polyphosphoinositide hydrolysis in the ventrobasal thalamus, whereas the expression of mGlu5 receptors was increased in the somatosensory cortex. Interestingly, these changes preceded the onset of the epileptic phenotype, being already visible in pre-symptomatic WAG/Rij rats. SWDs in symptomatic WAG/Rij rats were not influenced by pharmacological blockade of mGlu5 receptors with MTEP (10 or 30 mg/kg, i.p.), but were significantly decreased by mGlu5 receptor potentiation with the novel enhancer, VU0360172 (3 or 10 mg/kg, s.c.), without affecting motor behaviour. The effect of VU0360172 was prevented by co-treatment with MTEP. These findings suggest that changes in mGlu5 receptors might lie at the core of the absence-seizure prone phenotype of WAG/Rij rats, and that mGlu5 receptor enhancers are potential candidates to the treatment of absence epilepsy. This article is part of a Special Issue entitled 'Metabotropic Glutamate Receptors'. | Age Factors, Animals, Brain Waves, Cerebral Cortex, Disease Models, Animal, Electroencephalography, Epilepsy, Absence, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Hydrolysis, Male, Motor Activity, Niacinamide, Phosphatidylinositol Phosphates, Pyridines, Rats, Rats, Inbred Strains, Receptor, Metabotropic Glutamate 5, Receptors, Metabotropic Glutamate, Somatosensory Cortex, Thiazoles, Ventral Thalamic Nuclei | null |
22,705,342 | 2012-12-10 | 2012-08-03 | 1095-9947 | Fish & shellfish immunology | Expression pattern, promoter activity and bactericidal property of β-defensin from the mandarin fish Siniperca chuatsi. | Wang Gailing, Li Junhua, Zou Pengfei, Xie Haixia, Huang Bei, Nie Pin, Chang Mingxian | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fish Proteins, RNA, Messenger, Recombinant Proteins, beta-Defensins | IM | 22705342, S1050-4648(12)00206-9, 10.1016/j.fsi.2012.06.003 | β-Defensin (BD) are cysteine-rich, cationic antimicrobial peptides which play an important role in innate immune system against invading microbes. In the present study, the cDNA cloning, expression analysis, transcriptional regulation and antimicrobial activity of β-defensin (ScBD) from mandarin fish (Siniperca chuatsi) were characterized. The cDNA sequence of ScBD is 596 bp which encodes a protein of 63 amino acids (aa). The ScBD gene comprises three exons and two introns. The signal peptide is located in the first exon. ScBD contains 6 cysteines, and belongs to fish defensin 2 group based on phylogenetic analysis. Real-time quantitative PCR results showed that the mRNA transcripts of ScBD were distributed mainly in mucosal and lymphoid organs/tissues including intestine, gill, head kidney, kidney and spleen, with the highest level observed in spleen. Western blotting analysis revealed that the ScBD protein was abundant in head kidney, gill and spleen. A total of 3268 bp 5' flanking region of the ScBD gene promoter was sequenced, which contained a number of putative transcriptional binding sites for transcription factors. These transcription factors were analyzed using in vitro luciferase assay. The DNA region from position of -705 to -498 bp contains positive regulatory elements and that of -227 to +54 bp harbors the TATA which is essential for initiating gene expression. In addition, the ScBD peptide showed antibacterial activity against Escherichia coli M15, Staphylococcus aureus and Aeromonas hydrophila, whilst no effect on Edwardsiella tarda. These data suggest that the ScBD is importantly involved in host immune responses to invasion of bacterial pathogens. | Amino Acid Sequence, Animals, Bacteria, Bacterial Infections, Cloning, Molecular, Fish Diseases, Fish Proteins, Gene Expression Profiling, Gene Expression Regulation, Molecular Sequence Data, Organ Specificity, Perciformes, Polymerase Chain Reaction, RNA, Messenger, Recombinant Proteins, Sequence Alignment, Sequence Analysis, DNA, beta-Defensins | null |
22,705,343 | 2012-12-19 | 2016-11-25 | 1549-4713 | Ophthalmology | Cognitive impairment in age-related macular degeneration and geographic atrophy. | Woo Se Joon, Park Kyu Hyung, Ahn Jeeyun, Choe Jin Yeong, Jeong Hyeon, Han Ji Won, Kim Tae Hui, Kim Ki Woong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705343, S0161-6420(12)00398-3, 10.1016/j.ophtha.2012.04.026 | To investigate cognitive function in patients with early and late age-related macular degeneration (AMD) compared with an elderly, community-dwelling Korean population without AMD. | Aged, Case-Control Studies, Cognition, Cognitive Dysfunction, Educational Status, Female, Geographic Atrophy, Geriatric Assessment, Humans, Infant, Intelligence Tests, Macular Degeneration, Male, Odds Ratio, Risk Factors, Visual Acuity | null |
22,705,345 | 2012-12-19 | 2022-03-31 | 1549-4713 | Ophthalmology | Descemet's stripping endothelial keratoplasty: long-term graft survival and risk factors for failure in eyes with preexisting glaucoma. | Anshu Arundhati, Price Marianne O, Price Francis W | eng | null | Journal Article | null | IM | 22705345, S0161-6420(12)00403-4, 10.1016/j.ophtha.2012.04.031 | To evaluate long-term corneal graft survival and risk factors for graft failure after Descemet's stripping endothelial keratoplasty (DSEK) in eyes with preexisting glaucoma. | Adult, Aged, Aged, 80 and over, Case-Control Studies, Corneal Diseases, Corneal Endothelial Cell Loss, Descemet Stripping Endothelial Keratoplasty, Endothelium, Corneal, Female, Follow-Up Studies, Glaucoma, Graft Survival, Humans, Intraocular Pressure, Male, Middle Aged, Retrospective Studies, Risk Factors, Tissue Donors, Trabeculectomy, Treatment Failure, Young Adult | null |
22,705,344 | 2012-11-23 | 2023-12-13 | 1549-4713 | Ophthalmology | Heritability and genome-wide association study to assess genetic differences between advanced age-related macular degeneration subtypes. | Sobrin Lucia, Ripke Stephan, Yu Yi, Fagerness Jesen, Bhangale Tushar R, Tan Perciliz L, Souied Eric H, Buitendijk Gabriëlle H S, Merriam Joanna E, Richardson Andrea J, Raychaudhuri Soumya, Reynolds Robyn, Chin Kimberly A, Lee Aaron Y, Leveziel Nicolas, Zack Donald J, Campochiaro Peter, Smith R Theodore, Barile Gaetano R, Hogg Ruth E, Chakravarthy Usha, Behrens Timothy W, Uitterlinden André G, van Duijn Cornelia M, Vingerling Johannes R, Brantley Milam A, Baird Paul N, Klaver Caroline C W, Allikmets Rando, Katsanis Nicholas, Graham Robert R, Ioannidis John P A, Daly Mark J, Seddon Johanna M | eng | R01 EY013435 (NEI NIH HHS, United States); U01 MH046276 (NIMH NIH HHS, United States); R01 MH59588 (NIMH NIH HHS, United States); R01-EY11309 (NEI NIH HHS, United States); R01 MH59587 (NIMH NIH HHS, United States); K12-EY16335 (NEI NIH HHS, United States); U01 MH46289 (NIMH NIH HHS, United States); R01 MH059587 (NIMH NIH HHS, United States); R01 EY011309 (NEI NIH HHS, United States); U01 MH79469 (NIMH NIH HHS, United States); R01 MH059566 (NIMH NIH HHS, United States); R01 MH60870 (NIMH NIH HHS, United States); R01 MH81800 (NIMH NIH HHS, United States); R01 MH061675 (NIMH NIH HHS, United States); R01 MH60879 (NIMH NIH HHS, United States); R01 MH59566 (NIMH NIH HHS, United States); R01-EY13435 (NEI NIH HHS, United States); U01 MH79470 (NIMH NIH HHS, United States); K12 EY016335 (NEI NIH HHS, United States); U01 MH079469 (NIMH NIH HHS, United States); R01 MH59586 (NIMH NIH HHS, United States); R01 MH59565 (NIMH NIH HHS, United States); R01 MH067257 (NIMH NIH HHS, United States); EY012261 (NEI NIH HHS, United States); R01 MH060870 (NIMH NIH HHS, United States); R01 MH081800 (NIMH NIH HHS, United States); R01 MH61675 (NIMH NIH HHS, United States); R01 MH059571 (NIMH NIH HHS, United States); R01 MH059565 (NIMH NIH HHS, United States); R24 EY017404 (NEI NIH HHS, United States); U01 MH079470 (NIMH NIH HHS, United States); R01 MH59571 (NIMH NIH HHS, United States); EY012279 (NEI NIH HHS, United States); R01 MH059586 (NIMH NIH HHS, United States); R01 MH67257 (NIMH NIH HHS, United States); EY012211 (NEI NIH HHS, United States); R01 MH059588 (NIMH NIH HHS, United States); U01 MH046318 (NIMH NIH HHS, United States); R24-EY017404 (NEI NIH HHS, United States); R01 MH060879 (NIMH NIH HHS, United States) | Comparative Study, Journal Article, Meta-Analysis, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | ARMS2 protein, human, Proteins, High-Temperature Requirement A Serine Peptidase 1, HTRA1 protein, human, Serine Endopeptidases | IM | 22705344, S0161-6420(12)00239-4, 10.1016/j.ophtha.2012.03.014, PMC3899891, NIHMS364375, 19198609, 15761120, 9439359, 9869796, 18685559, 15078679, 18502512, 16458093, 21122828, 17053109, 16080115, 17767156, 21844367, 16862161, 20385819, 15870199, 19844262, 15761122, 19026761, 20385826, 20207970, 21917140, 9186125, 16818082, 17634448, 18061132, 16174643, 19117936, 17021323, 12945014, 16518403, 21670343, 15761121, 16281465, 17053108, 8827966, 21959373, 10721957, 17456821, 18776909, 21665990, 17728769, 16936732 | To investigate whether the 2 subtypes of advanced age-related macular degeneration (AMD), choroidal neovascularization (CNV), and geographic atrophy (GA) segregate separately in families and to identify which genetic variants are associated with these 2 subtypes. | Choroidal Neovascularization, Female, Genetic Predisposition to Disease, Genome-Wide Association Study, Genotype, Geographic Atrophy, High-Temperature Requirement A Serine Peptidase 1, Humans, Macular Degeneration, Male, Polymorphism, Single Nucleotide, Proteins, Risk Factors, Serine Endopeptidases, Siblings | null |
22,705,346 | 2012-12-19 | 2022-03-17 | 1549-4713 | Ophthalmology | Visual function after correction of distance refractive error with ready-made and custom spectacles: a randomized clinical trial. | Brady Christopher J, Villanti Andrea C, Gandhi Monica, Friedman David S, Keay Lisa | eng | null | Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | null | IM | 22705346, S0161-6420(12)00319-3, 10.1016/j.ophtha.2012.03.051 | To evaluate patient-reported outcome measures with the use of ready-made spectacles (RMS) and custom spectacles (CS) in an adult population in India with uncorrected refractive error (URE). | Adolescent, Adult, Double-Blind Method, Eyeglasses, Female, Follow-Up Studies, Humans, Male, Manufactured Materials, Middle Aged, Patient Satisfaction, Prescriptions, Prospective Studies, Quality of Life, Refraction, Ocular, Refractive Errors, Visual Acuity, Young Adult | null |
22,705,347 | 2012-10-08 | 2013-11-21 | 1873-2968 | Biochemical pharmacology | Fetal exposure to high isoflurane concentration induces postnatal memory and learning deficits in rats. | Kong Fei-Juan, Ma Lei-Lei, Hu Wen-Wen, Wang Wen-Na, Lu Hui-Shun, Chen Shu-Ping | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anesthetics, Inhalation, Isoflurane, Caspase 3 | IM | 22705347, S0006-2952(12)00398-X, 10.1016/j.bcp.2012.06.001 | We developed a maternal fetal rat model to study the effects of isoflurane-induced neurotoxicity on the fetuses of pregnant rats exposed in utero. Pregnant rats at gestational day 14 were exposed to 1.3 or 3% isoflurane for 1h. At postnatal day 28, spatial learning and memory of the offspring were examined using the Morris Water Maze. The apoptosis was evaluated by caspase-3 immunohistochemistry in the hippocampal CA1 region. Simultaneously, the ultrastructure changes of synapse in the hippocampal CA1 and dentate gyrus region were observed by transmission electron microscopy (TEM). The 3% isoflurane treatment group showed significantly longer escape latency, less time spent in the third quadrant and fewer original platform crossings in the Morris Water Maze test, significantly increased number and optical densities of caspase-3 neurons. This treatment also produced remarkable changes in synaptic ultrastructure compared with the control and the 1.3% isoflurane groups. There were no differences in the Morris Water Maze test, densities of caspase-3 positive cells, or synaptic ultrastructure between the control and 1.3% isoflurane groups. High isoflurane concentration (3%) exposure during pregnancy caused spatial memory and learning impairments and more neurodegeneration in the offspring rats compared with control or lower isoflurane concentrations. | Anesthetics, Inhalation, Animals, CA1 Region, Hippocampal, Caspase 3, Dose-Response Relationship, Drug, Female, Isoflurane, Learning, Maternal-Fetal Exchange, Maze Learning, Memory, Neurons, Pregnancy, Prenatal Exposure Delayed Effects, Rats | null |
22,705,350 | 2012-11-19 | 2022-03-10 | 0006-3002 | Biochimica et biophysica acta | Lysine 394 is a novel Rad6B-induced ubiquitination site on beta-catenin. | Gerard Brigitte, Sanders Matthew A, Visscher Daniel W, Tait Larry, Shekhar Malathy P V | eng | P30 CA022453 (NCI NIH HHS, United States) | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Armadillo Domain Proteins, CTNNB1 protein, human, Ubiquitin, beta Catenin, UBE2B protein, human, Ubiquitin-Conjugating Enzymes, Lysine | IM | 22705350, S0167-4889(12)00162-0, 10.1016/j.bbamcr.2012.05.032, PMC3915360, NIHMS519786, 15300008, 17081971, 3306404, 8548773, 19722269, 15472907, 16707478, 16293619, 1717990, 15473860, 10921899, 2850263, 15102669, 11280772, 21767405, 12000790, 9312064, 1658333, 10642555, 15060161, 1559696, 2157209, 10656974, 17456761, 17050667, 9233789, 10759547, 16849322, 2483723, 14739782, 8031302, 10228155, 11955436, 15048125, 9891886, 15476853, 8087846, 9407023, 3881753, 11929833, 11737884, 19914164, 18339854, 15064719, 10966653, 17172446, 13679587, 17121933 | The ubiquitin conjugating enzyme Rad6B is overexpressed in breast cancer and induces β-catenin transcriptional activation and stabilization via K63-linked polyubiquitination. Here we identify β-catenin and Rad6B interacting regions, identify potential Rad6B ubiquitination sites in β-catenin, and characterize their breast cancer tissue expression. β-catenin and Rad6B colocalize in breast carcinoma and coimmunoprecipitate from MDA-MB-231 cells. Pull-down assays using GST-β-catenin and His-Rad6B deletion mutants identified amino acids 131-181 and 50-116, respectively, as necessary for their interaction. Ubiquitination assays using β-catenin deletion mutants mapped Rad6B-induced ubiquitination within β-catenin 181-422 encompassing Armadillo repeats 2-7. Lysine to arginine mutations within repeats 5-7 identified K394 as the major Rad6B ubiquitination site in vitro and in vivo, and confirmed by Rad6B ubiquitination of a β-catenin peptide encompassing K394. Ubiquitination of wild type- but not K394R-β-catenin was decreased by Rad6B silencing. Compared to wild type-, K312R-, K335R-, K345R-, or K354R-β-catenin, K394R mutation caused ~50% drop in TOP/Flash activity in Wnt-silent MCF-7 cells. Consistent with these data, expression of Rad6B, itself a β-catenin/TCF transcriptional target, was also reduced in K394R-β-catenin transfected cells. Steady-state K394R-β-catenin levels are decreased compared to wild type-β-catenin. The decreased expression is not due to proteasomal degradation as treatment with MG132 failed to rescue its levels. Lymph node-positive breast carcinomas express higher levels of Rad6 protein and Rad6 activity, and K63-linked ubiquitinated β-catenin than reduction mammoplasties. These data suggest that K394 is a novel site of β-catenin ubiquitination that may be important for the stability and activity of β-catenin in breast cancer. | Armadillo Domain Proteins, Breast Neoplasms, Catalytic Domain, Cell Line, Tumor, Female, Gene Silencing, Humans, Lysine, Mutation, Neoplasm Invasiveness, Protein Binding, Transcription, Genetic, Ubiquitin, Ubiquitin-Conjugating Enzymes, Ubiquitination, Up-Regulation, beta Catenin | null |
22,705,351 | 2012-11-19 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | Molecular remodeling mechanisms of the neural somatodendritic compartment. | Moore Fletcher B, Baleja James D | eng | null | Journal Article, Review | Clathrin | IM | 22705351, S0167-4889(12)00161-9, 10.1016/j.bbamcr.2012.06.006 | Neuronal cells use the process of vesicle trafficking to manipulate the populations of neurotransmitter receptors and other membrane proteins. Long term potentiation (LTP) is a long-lived increase in synaptic strength between neurons and increases postsynaptic dendritic spine size and the concentration of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate-type glutamate receptor (AMPAR) located in the postsynaptic density. AMPAR is removed from the cell surface via clathrin-mediated endocytosis. While the adaptor protein 2 (AP2) complex of endocytosis seems to have the components needed to allow temporal and spatial regulations of internalization, many accessory proteins are involved, such as epidermal growth factor receptor phosphorylation substrate 15 (Eps15). A sequence of repeats in the Eps15 protein is known as the Eps15 homology (EH) domain. It has affinity for asparagine-proline-phenylalanine (NPF) sequences that are contained within vesicle trafficking proteins such as epsin, Rab11 family interacting protein 2 (Rab11-FIP2), and Numb. After endocytosis, a pool of AMPAR is stored in the endosomal recycling compartment that can be transported to the dendritic spine surface upon stimulation during LTP for lateral diffusion into the postsynaptic density. Rab11 and the Eps15 homologue EHD1 are involved in receptor recycling. EHD family members are also involved in transcytosis of the neuronal cell adhesion molecule neuron-glia cell adhesion molecule (NgCAM) from the somatodendritic compartment to the axon. Neurons have a unique morphology comprising many projections of membrane that is constructed in part by the effects of the Eps15 homologue, intersectin. Morphogenesis in the somatodendritic compartment is becoming better understood, but there is still much exciting territory to explore, especially regarding the roles of various EH domain-NPF interactions in endocytic and recycling processes. | Animals, Cell Compartmentation, Clathrin, Dendrites, Endocytosis, Humans, Models, Biological, Transport Vesicles | null |
22,705,348 | 2012-12-03 | 2012-07-30 | 1096-7206 | Molecular genetics and metabolism | A new variant of phosphoglycerate kinase deficiency (p.I371K) with multiple tissue involvement: molecular and functional characterization. | Fermo Elisa, Bianchi Paola, Chiarelli Laurent Roberto, Maggi Maristella, Mandarà Giuseppa Maria Luana, Vercellati Cristina, Marcello Anna Paola, Barcellini Wilma, Cortelezzi Agostino, Valentini Giovanna, Zanella Alberto | eng | null | Case Reports, Journal Article, Research Support, Non-U.S. Gov't | Isoenzymes, Mutant Proteins, RNA, Messenger, Phosphoglycerate Kinase, phosphoglycerate kinase, testis specific | IM | 22705348, S1096-7192(12)00204-1, 10.1016/j.ymgme.2012.05.015 | Phosphoglycerate kinase (PGK) is a key glycolytic enzyme that catalyzes the reversible phosphotransfer reaction from 1,3-bisphosphoglycerate to MgADP, to form 3-phosphoglycerate and MgATP. Two isozymes encoded by distinct genes are present in humans: PGK-1, located on Xq-13.3, encodes a ubiquitous protein of 417 amino acids, whereas PGK-2 is testis-specific. PGK1 deficiency is characterized by mild to severe hemolytic anemia, neurological dysfunctions and myopathy; patients rarely exhibit all three clinical features. Nearly 40 cases have been reported, 27 of them characterized at DNA or protein level, and 20 different mutations were described. Here we report the first Italian case of PGK deficiency characterized at a molecular and biochemical level. The patient presented during infancy with hemolytic anemia, increased CPK values, and respiratory distress; the study of red blood cell enzymes showed a drastic reduction in PGK activity. In adulthood he displayed mild hemolytic anemia, mental retardation and severe myopathy. PGK-1 gene sequencing revealed the new missense mutation c.1112T>A (p.Ile371Lys). The mutation was not found among 100 normal alleles, and even if located in the third to the last nucleotide of exon 9, it did not alter mRNA splicing. The p.Ile371Lys mutation falls in a conserved region of the enzyme, near the nucleotide binding site. The mutant enzyme shows reduced catalytic rates toward both substrates (apparent k(cat) values, 12-fold lower than wild-type) and a decreased affinity toward MgATP (apparent K(m), 6-fold higher than wild-type). Moreover, it lost half of activity after nearly 9-min incubation at 45°C, a temperature that did not affect the wild-type enzyme (t(1/2)>1 h). The possible compensatory expression of PGK2 isoenzyme was investigated in the proband and in the heterozygote healthy sisters, and found to be absent. Therefore, the highly perturbed catalytic properties of the new variant p.Ile371Lys, combined with protein instability, account for the PGK deficiency found in the patient and correlate with the clinical expression of the disease. | Adult, Amino Acid Sequence, Child, Preschool, Enzyme Activation, Enzyme Stability, Female, Gene Expression Regulation, Enzymologic, Genetic Diseases, X-Linked, Humans, Infant, Infant, Newborn, Isoenzymes, Kinetics, Male, Metabolism, Inborn Errors, Molecular Sequence Data, Mutant Proteins, Mutation, Organ Specificity, Pedigree, Phosphoglycerate Kinase, RNA, Messenger | null |
22,705,349 | 2012-12-04 | 2012-08-06 | 1873-6254 | Acta tropica | The genetic diversity of Plasmodium malariae and Plasmodium brasilianum from human, simian and mosquito hosts in Brazil. | Guimarães L O, Bajay M M, Wunderlich G, Bueno M G, Röhe F, Catão-Dias J L, Neves A, Malafronte R S, Curado I, Kirchgatter K | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705349, S0001-706X(12)00226-4, 10.1016/j.actatropica.2012.05.016 | Plasmodium malariae is a protozoan parasite that causes malaria in humans and is genetically indistinguishable from Plasmodium brasilianum, a parasite infecting New World monkeys in Central and South America. P. malariae has a wide and patchy global distribution in tropical and subtropical regions, being found in South America, Asia, and Africa. However, little is known regarding the genetics of these parasites and the similarity between them could be because until now there are only a very few genomic sequences available from simian Plasmodium species. This study presents the first molecular epidemiological data for P. malariae and P. brasilianum from Brazil obtained from different hosts and uses them to explore the genetic diversity in relation to geographical origin and hosts. By using microsatellite genotyping, we discovered that of the 14 human samples obtained from areas of the Atlantic forest, 5 different multilocus genotypes were recorded, while in a sample from an infected mosquito from the same region a different haplotype was found. We also analyzed the longitudinal change of circulating plasmodial genetic profile in two untreated non-symptomatic patients during a 12-months interval. The circulating genotypes in the two samples from the same patient presented nearly identical multilocus haplotypes (differing by a single locus). The more frequent haplotype persisted for almost 3 years in the human population. The allele Pm09-299 described previously as a genetic marker for South American P. malariae was not found in our samples. Of the 3 non-human primate samples from the Amazon Region, 3 different multilocus genotypes were recorded indicating a greater diversity among isolates of P. brasilianum compared to P. malariae and thus, P. malariae might in fact derive from P. brasilianum as has been proposed in recent studies. Taken together, our data show that based on the microsatellite data there is a relatively restricted polymorphism of P. malariae parasites as opposed to other geographic locations. | Alleles, Animals, Brazil, Culicidae, Genetic Variation, Genotype, Haplorhini, Humans, Malaria, Microsatellite Repeats, Molecular Epidemiology, Molecular Sequence Data, Multilocus Sequence Typing, Plasmodium, Primate Diseases | null |
22,705,352 | 2012-12-11 | 2021-10-21 | 0006-3002 | Biochimica et biophysica acta | Bioluminescence assay platform for selective and sensitive detection of Ub/Ubl proteases. | Orcutt Steven J, Wu Jian, Eddins Michael J, Leach Craig A, Strickler James E | eng | R43 GM090511 (NIGMS NIH HHS, United States); 1R43GM090511-01A1 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | NEDD8 Protein, NEDD8 protein, human, SUMO2 protein, human, Small Ubiquitin-Related Modifier Proteins, Ubiquitin, Ubiquitins, Endopeptidases, Proteasome Endopeptidase Complex | IM | 22705352, S0167-4889(12)00159-0, 10.1016/j.bbamcr.2012.06.004, PMC3465522, NIHMS386724, 16905103, 19007433, 18482987, 16338347, 18424514, 19543231, 16964971, 19626045, 9485312, 17381419, 21955996, 15799957, 19948475, 9759494, 17355205, 10838414, 12507430, 15567417, 18729020, 8533602, 19489724, 10882122, 15296745, 17869210, 21133675, 20829789, 16325574, 18852458, 19345192 | As the importance of ubiquitylation in certain disease states becomes increasingly apparent, the enzymes responsible for removal of ubiquitin (Ub) from target proteins, deubiquitylases (DUBs), are becoming attractive targets for drug discovery. For rapid identification of compounds that alter DUB function, in vitro assays must be able to provide statistically robust data over a wide dynamic range of both substrate and enzyme concentrations during high throughput screening (HTS). The most established reagents for HTS are Ubs with a quenched fluorophore conjugated to the C-terminus; however, a luciferase-based strategy for detecting DUB activity (DUB-Glo™, Promega) provides a wider dynamic range than traditional fluorogenic reagents. Unfortunately, this assay requires high enzyme concentrations and lacks specificity for DUBs over other isopeptidases (e.g. desumoylases), as it is based on an aminoluciferin (AML) derivative of a peptide derived from the C-terminus of Ub (Z-RLRGG-). Conjugation of aminoluciferin to a full-length Ub (Ub-AML) yields a substrate that has a wide dynamic range, yet displays detection limits for DUBs 100- to 1000-fold lower than observed with DUB-Glo™. Ub-AML was even a sensitive substrate for DUBs (e.g. JosD1 and USP14) that do not show appreciable activity with DUB-Glo™. Aminoluciferin derivatives of hSUMO2 and NEDD8 were also shown to be sensitive substrates for desumoylases and deneddylases, respectively. Ub/Ubl-AML substrates are amenable to HTS (Z'=0.67) yielding robust signal, and providing an alternative drug discovery platform for Ub/Ubl isopeptidases. This article is part of a Special Issue entitled: Ubiquitin Drug Discovery and Diagnostics. | Endopeptidases, High-Throughput Screening Assays, Humans, Luminescent Measurements, NEDD8 Protein, Proteasome Endopeptidase Complex, Sensitivity and Specificity, Small Ubiquitin-Related Modifier Proteins, Ubiquitin, Ubiquitins | null |
22,705,353 | 2013-03-19 | 2016-11-26 | 0006-3002 | Biochimica et biophysica acta | Chaperone-protease networks in mitochondrial protein homeostasis. | Voos Wolfgang | eng | null | Journal Article, Review | Molecular Chaperones, Proteins, Peptide Hydrolases | IM | 22705353, S0167-4889(12)00160-7, 10.1016/j.bbamcr.2012.06.005 | As essential organelles, mitochondria are intimately integrated into the metabolism of a eukaryotic cell. The maintenance of the functional integrity of the mitochondrial proteome, also termed protein homeostasis, is facing many challenges both under normal and pathological conditions. First, since mitochondria are derived from bacterial ancestor cells, the proteins in this endosymbiotic organelle have a mixed origin. Only a few proteins are encoded on the mitochondrial genome, most genes for mitochondrial proteins reside in the nuclear genome of the host cell. This distribution requires a complex biogenesis of mitochondrial proteins, which are mostly synthesized in the cytosol and need to be imported into the organelle. Mitochondrial protein biogenesis usually therefore comprises complex folding and assembly processes to reach an enzymatically active state. In addition, specific protein quality control (PQC) processes avoid an accumulation of damaged or surplus polypeptides. Mitochondrial protein homeostasis is based on endogenous enzymatic components comprising a diverse set of chaperones and proteases that form an interconnected functional network. This review describes the different types of mitochondrial proteins with chaperone functions and covers the current knowledge of their roles in protein biogenesis, folding, proteolytic removal and prevention of aggregation, the principal reactions of protein homeostasis. This article is part of a Special Issue entitled: Protein Import and Quality Control in Mitochondria and Plastids. | Homeostasis, Mitochondria, Molecular Chaperones, Peptide Hydrolases, Proteins, Quality Control | null |
22,705,355 | 2013-01-24 | 2022-03-30 | 1479-666X | The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland | Transforaminal endoscopic spinal surgery: the future 'gold standard' for discectomy? - A review. | Gibson J N Alastair, Cowie Jonathan G, Iprenburg Menno | eng | null | Journal Article, Review | null | IM | 22705355, S1479-666X(12)00055-8, 10.1016/j.surge.2012.05.001 | Lumbar disc prolapse is common and the primary method of care in most centres is still open discectomy facilitated by microscope or loupe magnification and illumination. Hospitalisation may be less than 24 h, but post-operative pain usually requires an overnight stay. This review describes transforaminal endoscopic spinal surgery (TESS) using HD-video technology, that is generally performed as a day case procedure under sedation or light general anaesthesia, and collates the evidence comparing the technique to microdiscectomy. | Cost Savings, Diskectomy, Endoscopy, Humans, Intervertebral Disc Displacement, Learning Curve, Lumbar Vertebrae, Operating Rooms, Patient Satisfaction, Return to Work, Treatment Outcome | null |
22,705,356 | 2013-05-29 | 2016-11-25 | 1879-0593 | Oral oncology | Novel MDM2 splice variants identified from oral squamous cell carcinoma. | Sam Kin Kit, Gan Chai Phei, Yee Pei San, Chong Chan Eng, Lim Kue Peng, Karen-Ng Lee Peng, Chang Wei Sern, Nathan Sheila, Rahman Zainal Ariff Abdul, Ismail Siti Mazlipah, Cheong Sok Ching | eng | null | Journal Article, Research Support, Non-U.S. Gov't | MDM2 protein, human, Proto-Oncogene Proteins c-mdm2 | IM | 22705356, S1368-8375(12)00180-7, 10.1016/j.oraloncology.2012.05.016 | The presence of a variety of MDM2 splice variants has been reported in a range of different tumor types and is associated with poor patient prognosis. Furthermore, several MDM2 variants have been shown to have oncogenic properties. Despite this, MDM2 splice variants have not been comprehensively characterized in oral squamous cell carcinoma (OSCC). | Carcinoma, Squamous Cell, Cell Transformation, Neoplastic, Genes, p53, Humans, Lymphatic Metastasis, Mouth Mucosa, Mouth Neoplasms, Mutation, Polymerase Chain Reaction, Proto-Oncogene Proteins c-mdm2, RNA Splicing | null |
22,705,357 | 2012-12-27 | 2018-12-01 | 1879-0593 | Oral oncology | Zinc and vitamin A as a low cost management of oral submucous fibrosis: comment on Chole RH et al. "Review of drug treatment of oral submucous fibrosis. Oral Oncol 2012; 48(5):393-398". | Mukherjee Sanjit, Ray Jay Gopal, Chaudhuri Keya | eng | null | Letter, Comment | null | IM | 22705357, S1368-8375(12)00183-2, 10.1016/j.oraloncology.2012.05.018 | null | Clinical Trials as Topic, Female, Humans, Male, Oral Submucous Fibrosis | null |
22,705,358 | 2013-07-12 | 2021-12-03 | 1873-4758 | The International journal on drug policy | Travel distance to outpatient substance use disorder treatment facilities for Spanish-speaking clients. | Guerrero Erick G, Kao Dennis, Perron Brian E | eng | R24 DA019798 (NIDA NIH HHS, United States) | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705358, S0955-3959(12)00073-4, 10.1016/j.drugpo.2012.04.004, PMC4121956, NIHMS609098, 14987337, 19284669, 18432387, 15939838, 17325113, 12668754, 21680111, 17499958, 18234088, 20727703, 12042604, 17207101, 14693257, 7484987, 18528760, 11729020, 21835041, 20602756, 19019434, 10857471, 21057070, 20441453, 15234946, 12834661 | Travel distance and English proficiency skills are widely recognized factors associated with service access and treatment engagement. As Latino populations represent one of the most rapidly growing populations in the United States, methods are needed to better understand availability of linguistically appropriate services in Latino communities. Given regional variability in the density of Latino communities, the current study examines treatment access as travel distance to outpatient substance use disorder treatment facilities in one of the largest and most rapidly changing Latino communities in the United States - Los Angeles County, CA. | Ambulatory Care, California, Censuses, Cluster Analysis, Cultural Characteristics, Geographic Information Systems, Health Behavior, Health Services Accessibility, Health Services Needs and Demand, Hispanic or Latino, Humans, Language, Poverty, Residence Characteristics, Substance Abuse Treatment Centers, Substance-Related Disorders, Travel | null |
22,705,359 | 2012-11-26 | 2015-11-19 | 1878-1705 | International immunopharmacology | Molecular mechanisms of anti-inflammatory action of the flavonoid, tricin from Njavara rice (Oryza sativa L.) in human peripheral blood mononuclear cells: possible role in the inflammatory signaling. | Shalini V, Bhaskar Shobha, Kumar Kavitha S, Mohanlal Smitha, Jayalekshmy Ananthasankaran, Helen Antony | eng | null | Journal Article | Anti-Inflammatory Agents, Non-Steroidal, Flavonoids, Inflammation Mediators, Interleukin-6, Lipopolysaccharides, NF-kappa B, Tumor Necrosis Factor-alpha, Nitric Oxide, tricin, Dinoprostone | IM | 22705359, S1567-5769(12)00174-9, 10.1016/j.intimp.2012.06.005 | Flavonoids are a group of natural substances that are located in sources of vegetal origin and are able to regulate acute and chronic inflammatory responses. The anti-oxidant and anti-inflammatory effects corroborate with the preferential use of Njavara, a rice variety in indigenous medicine and the phytochemical investigations revealed the occurrence of a flavonoid, tricin at significantly higher levels compared to staple varieties. This study describes the new aspects of inflammatory suppression by the Njavara rice by evaluating the role of active constituent, tricin in the regulation of production of various pro-inflammatory markers by human peripheral blood mononuclear cells stimulated with lipopolysaccharide. Treatment with tricin resulted in significant down-regulation of LPS-elicited production of TNF-α, IL-6, PGE(2) and NO. Tricin was found to be a potential blocker of the expression of isoforms of nitric oxide synthase, cyclooxygenase and matrix metalloproteinases. Modulation of the cascade of molecular events in lipopolysaccharide signaling also includes inhibition of transcription factor NF-κB evidenced by the detection of enhanced p65 subunit in the nuclear extracts on tricin supplementation. The present study summarizes the role of the flavonoid, tricin in the modulation of the expression of different inflammatory mediators and revealed that the inhibitory effects on cell signaling pathways are responsible for its anti-inflammatory activity. | Anti-Inflammatory Agents, Non-Steroidal, Cells, Cultured, Dinoprostone, Enzyme Activation, Flavonoids, Gene Expression Regulation, Humans, Inflammation Mediators, Interleukin-6, Leukocytes, Mononuclear, Lipopolysaccharides, NF-kappa B, Nitric Oxide, Oryza, Signal Transduction, Tumor Necrosis Factor-alpha | null |
22,705,360 | 2013-02-06 | 2013-11-21 | 1095-6859 | Gynecologic oncology | Treatment of high-risk gestational trophoblastic neoplasia with weekly high-dose methotrexate-etoposide. | Han Sileny N, Amant Frédéric, Leunen Karin, Devi Uma K, Neven Patrick, Berteloot Patrick, Vergote Ignace | eng | null | Journal Article | Etoposide, Methotrexate | IM | 22705360, S0090-8258(12)00452-0, 10.1016/j.ygyno.2012.06.014 | To assess toxicity and efficacy of weekly high-dose methotrexate-etoposide (HD MTX-ETO) in high-risk gestational trophoblastic neoplasia (GTN). | Adult, Antineoplastic Combined Chemotherapy Protocols, Dose-Response Relationship, Drug, Etoposide, Female, Gestational Trophoblastic Disease, Humans, Methotrexate, Pregnancy, Retrospective Studies | null |
22,705,354 | 2013-12-23 | 2014-07-07 | 2254-8874 | Revista clinica espanola | [Secondary cardiac tamponade to spontaneous hemomediastinum in relation with low molecular weight heparin]. | Ruiz-Salas A, Navarro-Arce I, Morcillo-Hidalgo L, Melero-Tejedor J M | spa | null | Case Reports, Letter | Anticoagulants, Heparin, Low-Molecular-Weight | IM | 22705354, S0014-2565(12)00214-7, 10.1016/j.rce.2012.04.013 | null | Anticoagulants, Cardiac Tamponade, Hemothorax, Heparin, Low-Molecular-Weight, Humans, Male, Mediastinal Diseases, Middle Aged | null |
22,705,361 | 2012-12-03 | 2022-03-17 | 1095-9319 | Microvascular research | On the importance of the submicrovascular network in a computational model of tumour growth. | Lesart Anne-Cécile, van der Sanden Boudewijn, Hamard Lauriane, Estève François, Stéphanou Angélique | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Vascular Endothelial Growth Factor A, vascular endothelial growth factor A, mouse, Oxygen | IM | 22705361, S0026-2862(12)00112-4, 10.1016/j.mvr.2012.06.001 | A computational model is potentially a powerful tool to apprehend complex phenomena like solid tumour growth and to predict the outcome of therapies. To that end, the confrontation of the model with experiments is essential to validate this tool. In this study, we develop a computational model specifically dedicated to the interpretation of tumour growth as observed in a mouse model with a dorsal skinfold chamber. Observation of the skin vasculature at the dorsal window scale shows a sparse network of a few main vessels of several hundreds micrometers in diameter. However observation at a smaller scale reveals the presence of a dense and regular interconnected network of capillaries about ten times smaller. We conveniently designate this structure as the submicrovascular network (SMVN).(1) The question that we wish to answer concerns the necessity of explicitly taking into account the SMVN in the computational model to describe the tumour evolution observed in the dorsal chamber. For that, simulations of tumour growth realised with and without the SMVN are compared and lead to two distinct scenarios. Parameters that are known to strongly influence the tumour evolution are then tested in the two cases to determine to which extent those parameters can be used to compensate the observed differences between these scenarios. Explicit modelling of the smallest vessels appears mandatory although not necessarily under the form of a regular grid. A compromise between the two investigated cases can thus be reached. | Animals, Apoptosis, Capillaries, Cell Hypoxia, Cell Proliferation, Computer Simulation, Glioma, Mice, Mice, Nude, Models, Cardiovascular, Necrosis, Neovascularization, Pathologic, Oxygen, Reproducibility of Results, Skin, Time Factors, Tumor Burden, Vascular Endothelial Growth Factor A | null |
22,705,362 | 2012-12-03 | 2015-11-19 | 1095-9319 | Microvascular research | The radiosensitivity of endothelial cells isolated from human breast cancer and normal tissue in vitro. | Park Moon-Taek, Oh Eun-Taex, Song Min-Jeong, Kim Woo-Jean, Cho Young Up, Kim Sei Joong, Han Jee-Young, Suh Jun-Kyu, Choi Eun Kyung, Lim Byung Uk, Song Chang Won, Park Heon Joo | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Biomarkers | IM | 22705362, S0026-2862(12)00113-6, 10.1016/j.mvr.2012.06.002 | We developed a novel method for harvesting endothelial cells from blood vessels of freshly obtained cancer and adjacent normal tissue of human breast, and compared the response of the cancer-derived endothelial cells (CECs) and normal tissue-derived endothelial cells (NECs) to ionizing radiation. In brief, when tissues were embedded in Matrigel and cultured in endothelial cell culture medium (ECM) containing growth factors, endothelial cells grew out of the tissues. The endothelial cells were harvested and cultured as monolayer cells in plates coated with gelatin, and the cells of 2nd-5th passages were used for experiments. Both CECs and NECs expressed almost the same levels of surface markers CD31, CD105 and TEM-8 (tumor endothelial marker-8), which are known to be expressed in angiogenic endothelial cells, i.e., mitotically active endothelial cells. Furthermore, both CECs and NECs were able to migrate into experimental wound in the monolayer culture, and also to form capillary-like tubes on Matrigel-coated plates. However, the radiation-induced suppressions of migration and capillary-like tube formations were greater for CECs than NECs from the same patients. In addition, in vitro clonogenic survival assays demonstrated that CECs were far more radiosensitive than NECs. In summary, we have developed a simple and efficient new method for isolating endothelial cells from cancer and normal tissue, and demonstrated for the first time that endothelial cells of human breast cancer are significantly more radiosensitive than their normal counterparts from the same patients. | Biomarkers, Breast, Breast Neoplasms, Cell Movement, Cell Separation, Cell Survival, Cells, Cultured, Dose-Response Relationship, Radiation, Endothelial Cells, Female, Humans, Neovascularization, Physiologic, Radiation Tolerance, Time Factors, Tissue Culture Techniques | null |
22,705,363 | 2013-05-17 | 2021-10-21 | 1872-7123 | Psychiatry research | Upregulation of adenosine A2A receptors induced by atypical antipsychotics and its correlation with sensory gating in schizophrenia patients. | Zhang Jie, Abdallah Chadi G, Wang Junqing, Wan Xiaona, Liang Chunlian, Jiang Liyun, Liu Yuzhen, Huang Haixing, Hong Xiaohong, Huang Qingjun, Wu Renhua, Xu Chongtao | eng | T32 DA022975 (NIDA NIH HHS, United States) | Journal Article, Research Support, Non-U.S. Gov't | Antipsychotic Agents, Dibenzothiazepines, Piperazines, Quinolones, Receptor, Adenosine A2A, Benzodiazepines, Quetiapine Fumarate, Aripiprazole, Risperidone, Olanzapine | IM | 22705363, S0165-1781(12)00206-5, 10.1016/j.psychres.2012.04.021, PMC3449024, NIHMS374250, 484737, 1932393, 9262169, 7972604, 21437008, 7910754, 12145486, 10784470, 2375945, 12827647, 12900046, 15329307, 1645590, 16278075, 10501634, 11137880, 8393482, 12634474, 11490180, 16431220, 9160120, 16580767, 18762587, 9342776, 6134559, 9885784, 8238642, 10790742, 8481748, 9767181, 9789908, 18395700, 690092, 11592813, 21315743, 7112520, 11872740, 10849191, 14645502, 7595490, 20363872, 18241306, 19106034, 1848997, 12377399, 15465979, 10330379, 11350540, 14515197, 9613620, 12469867, 2920190 | Sensory gating deficits have been found in patients with schizophrenia and their unaffected relatives. However, the underlying neurobiological mechanism of this deficit remains unclear. Pre-clinical studies have implicated adenosine in sensory gating deficits in schizophrenia. Therefore, the current study investigated a possible relationship between peripheral adenosine A2A receptor (ADORA2A) and sensory gating indices (P50 measures) in medication-free schizophrenia (n=31) and healthy (n=21) groups. The effects of six-week antipsychotic treatment were examined. At baseline, schizophrenia patients showed impaired sensory gating compared to healthy controls. However, there was no significant difference in ADORA2A gene expression among groups. In addition, ADORA2A expression was not correlated with sensory gating at any time point. Following treatment, we found a significant upregulation of ADORA2A expression. Intriguingly, we observed a significant positive association between ADORA2A upregulation and baseline P50 amplitudes in the schizophrenia group. A main finding of the current pilot study is the upregulation of ADORA2A expression following treatment with antipsychotics. In addition, this upregulation was predicted by baseline P50 amplitude, an observation that awaits replication in an expanded sample. | Acoustic Stimulation, Adolescent, Adult, Antipsychotic Agents, Aripiprazole, Auditory Cortex, Benzodiazepines, Dibenzothiazepines, Electroencephalography, Evoked Potentials, Auditory, Female, Humans, Male, Olanzapine, Piperazines, Quetiapine Fumarate, Quinolones, Reaction Time, Receptor, Adenosine A2A, Risperidone, Schizophrenia, Sensory Gating, Up-Regulation | null |
22,705,364 | 2012-12-21 | 2019-12-10 | 1873-4596 | Free radical biology & medicine | Improvement of iron-mediated oxidative DNA damage in patients with transfusion-dependent myelodysplastic syndrome by treatment with deferasirox. | Kikuchi Shohei, Kobune Masayoshi, Iyama Satoshi, Sato Tsutomu, Murase Kazuyuki, Kawano Yutaka, Takada Kohichi, Ono Kaoru, Kaneko Yumiko, Miyanishi Koji, Sato Yasushi, Hayashi Tsuyoshi, Takimoto Rishu, Kato Junji | eng | null | Journal Article | Antigens, CD, Benzoates, Iron Chelating Agents, Reactive Oxygen Species, Triazoles, 8-Hydroxy-2'-Deoxyguanosine, Ferritins, Deoxyguanosine, Deferasirox | IM | 22705364, S0891-5849(12)00341-3, 10.1016/j.freeradbiomed.2012.06.006 | Myelodysplastic syndrome (MDS) is characterized by dysplastic and ineffective hematopoiesis, peripheral blood cytopenias, and a risk of leukemic transformation. Most MDS patients eventually require red blood cell (RBC) transfusions for anemia and consequently develop iron overload. Excess free iron in cells catalyzes generation of reactive oxygen species that cause oxidative stress, including oxidative DNA damage. However, it is uncertain how iron-mediated oxidative stress affects the pathophysiology of MDS. This study included MDS patients who visited our university hospital and affiliated hospitals (n=43). Among them, 13 patients received iron chelation therapy when their serum ferritin (SF) level was greater than 1000 ng/mL or they required more than 20 RBC transfusions (or 100 mL/kg of RBC). We prospectively analyzed 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in peripheral blood mononuclear cells (PBMC) obtained from MDS patients before and after iron chelator, deferasirox, administration. We showed that the 8-OHdG levels in MDS patients were significantly higher than those in healthy volunteers and were positively correlated with SF and chromosomal abnormalities. Importantly, the 8-OHdG levels in PBMC of MDS patients significantly decreased after deferasirox administration, suggesting that iron chelation reduced oxidative DNA damage. Thus, excess iron could contribute to the pathophysiology of MDS and iron chelation therapy could improve the oxidative DNA damage in MDS patients. | 8-Hydroxy-2'-Deoxyguanosine, Antigens, CD, Benzoates, Case-Control Studies, DNA Damage, Deferasirox, Deoxyguanosine, Erythrocyte Transfusion, Ferritins, Genome, Human, Humans, Iron Chelating Agents, Iron Overload, Leukocytes, Mononuclear, Myelodysplastic Syndromes, Prospective Studies, Reactive Oxygen Species, Statistics, Nonparametric, Triazoles | null |
22,705,366 | 2012-12-21 | 2013-11-21 | 1873-4596 | Free radical biology & medicine | Carbonylation of the cytoskeletal protein actin leads to aggregate formation. | Castro Jose Pedro, Ott Christiane, Jung Tobias, Grune Tilman, Almeida Henrique | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Actins, Oxidants, Hydrogen Peroxide, Proteasome Endopeptidase Complex | IM | 22705366, S0891-5849(12)00340-1, 10.1016/j.freeradbiomed.2012.06.005 | Protein carbonylation is a common feature in cells exposed to oxidants, leading to protein dysfunction and protein aggregates. Actin, which is involved in manifold cellular processes, is a sensitive target protein to this oxidative modification. T-cell proteins have been widely described to be sensitive targets to oxidative modifications. The aim of this work was to test whether the formation of protein aggregates contributes to the impaired proliferation of Jurkat cells after oxidative stress and to test whether actin as a major oxidation-prone cytoskeletal protein is an integral part of such protein aggregates. We used Jurkat cells, an established T-cell model, showing the formation of actin aggregates along with the decrease of proteasome activity. The presence of these protein aggregates inhibits Jurkat proliferation even under conditions not influencing viability. As a conclusion, we propose that an oxidative environment leads to actin aggregates contributing to T-cell cellular functional impairment. | Actins, Cell Cycle Checkpoints, Cell Proliferation, Cell Survival, Humans, Hydrogen Peroxide, Jurkat Cells, Oxidants, Oxidative Stress, Proteasome Endopeptidase Complex, Protein Carbonylation, Protein Stability, Protein Transport, T-Lymphocytes | null |
22,705,365 | 2012-12-21 | 2022-03-30 | 1873-4596 | Free radical biology & medicine | Oxidative stress and metal carcinogenesis. | Lee Jeong-Chae, Son Young-Ok, Pratheeshkumar Poyil, Shi Xianglin | eng | 1R01ES015518 (NIEHS NIH HHS, United States); 1R01ES017244 (NIEHS NIH HHS, United States); 2R01AA015407 (NIAAA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Review | Environmental Pollutants, Metals, Reactive Oxygen Species | IM | 22705365, S0891-5849(12)00337-1, 10.1016/j.freeradbiomed.2012.06.002 | Occupational and environmental exposures to metals are closely associated with an increased risk of various cancers. Although carcinogenesis caused by metals has been intensively investigated, the exact mechanisms of action are still unclear. Accumulating evidence indicates that reactive oxygen species (ROS) generated by metals play important roles in the etiology of degenerative and chronic diseases. This review covers recent advances in (1) metal-induced generation of ROS and the related mechanisms; (2) the relationship between metal-mediated ROS generation and carcinogenesis; and (3) the signaling proteins involved in metal-induced carcinogenesis, especially intracellular reduction-oxidation-sensitive molecules. | Animals, Cell Transformation, Neoplastic, DNA Damage, Environmental Pollutants, Humans, Metals, Neoplasms, Oxidation-Reduction, Oxidative Stress, Reactive Oxygen Species, Signal Transduction | null |
22,705,367 | 2012-12-21 | 2021-10-21 | 1873-4596 | Free radical biology & medicine | Small amounts of isotope-reinforced polyunsaturated fatty acids suppress lipid autoxidation. | Hill Shauna, Lamberson Connor R, Xu Libin, To Randy, Tsui Hui S, Shmanai Vadim V, Bekish Andrei V, Awad Agape M, Marbois Beth N, Cantor Charles R, Porter Ned A, Clarke Catherine F, Shchepinov Mikhail S | eng | R01 GM045952 (NIGMS NIH HHS, United States); R01 HD064727 (NICHD NIH HHS, United States); HD064727 (NICHD NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Antioxidants, Oxidants, Ubiquinone, Arachidonic Acid, Copper, Linoleic Acid, Eicosapentaenoic Acid, Deuterium | IM | 22705367, S0891-5849(12)00339-5, 10.1016/j.freeradbiomed.2012.06.004, PMC3437768, NIHMS386954, 21848345, 3023384, 20955788, 20353821, 21861450, 9266513, 2687232, 16027161, 17378752, 21942677, 21906664, 19500666, 21766791, 15966710, 3541567, 12160930, 19705847, 13889855, 18285329, 8755509, 14680206, 8434935, 18489912, 21936577, 21600979, 20423158, 16626136, 9433901, 6351312, 16491182, 18158180, 590513, 17643134, 19543446, 21708248, 19224349, 17395012, 1937131, 16798075, 20647757, 17753813, 19166334, 8899983 | Polyunsaturated fatty acids (PUFAs) undergo autoxidation and generate reactive carbonyl compounds that are toxic to cells and associated with apoptotic cell death, age-related neurodegenerative diseases, and atherosclerosis. PUFA autoxidation is initiated by the abstraction of bis-allylic hydrogen atoms. Replacement of the bis-allylic hydrogen atoms with deuterium atoms (termed site-specific isotope-reinforcement) arrests PUFA autoxidation due to the isotope effect. Kinetic competition experiments show that the kinetic isotope effect for the propagation rate constant of Lin autoxidation compared to that of 11,11-D(2)-Lin is 12.8 ± 0.6. We investigate the effects of different isotope-reinforced PUFAs and natural PUFAs on the viability of coenzyme Q-deficient Saccharomyces cerevisiae coq mutants and wild-type yeast subjected to copper stress. Cells treated with a C11-BODIPY fluorescent probe to monitor lipid oxidation products show that lipid peroxidation precedes the loss of viability due to H-PUFA toxicity. We show that replacement of just one bis-allylic hydrogen atom with deuterium is sufficient to arrest lipid autoxidation. In contrast, PUFAs reinforced with two deuterium atoms at mono-allylic sites remain susceptible to autoxidation. Surprisingly, yeast treated with a mixture of approximately 20%:80% isotope-reinforced D-PUFA:natural H-PUFA are protected from lipid autoxidation-mediated cell killing. The findings reported here show that inclusion of only a small fraction of PUFAs deuterated at the bis-allylic sites is sufficient to profoundly inhibit the chain reaction of nondeuterated PUFAs in yeast. | Antioxidants, Arachidonic Acid, Copper, Deuterium, Eicosapentaenoic Acid, Kinetics, Linoleic Acid, Lipid Peroxidation, Oxidants, Oxidation-Reduction, Oxidative Stress, Saccharomyces cerevisiae, Ubiquinone | null |
22,705,368 | 2012-12-21 | 2022-04-09 | 1873-4596 | Free radical biology & medicine | Effects of sperm DNA damage on the levels of RAD51 and p53 proteins in zygotes and 2-cell embryos sired by golden hamsters without the major accessory sex glands. | Chen Hong, Liao Su-Bin, Cheung May P L, Chow Pak Ham, Cheung Annie L M, O Wai Sum | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Tumor Suppressor Protein p53, Rad51 Recombinase | IM | 22705368, S0891-5849(12)00342-5, 10.1016/j.freeradbiomed.2012.06.007 | We previously reported that the male accessory sex gland (ASG) secretion is the main source of antioxidants to safeguard sperm genomic integrity and functional competence. Removal of all ASGs in the golden hamster can reduce male fertility by increasing embryo wastage. This study aims to investigate whether the oxidative DNA-damaged sperm from hamsters without all ASGs (TX) could successfully fertilize oocytes and to qualify the status of DNA repair by the expression of RAD51 and p53 proteins. Here we demonstrated a significantly higher DNA-base adduct formation (8-hydroxy-2'-deoxyguanosine) in sperm from TX males than those from sham-operated males. Comet assays demonstrated that all female pronuclei in both zygotes were intact, but single- and double-strand DNA damage was found in decondensed sperm in TX males only. DNA damage could also be detected in both nuclei of the TX 2-cell embryos. RAD51, a DNA repair enzyme, was found to be evenly distributed in the cytoplasm and nuclei in oocytes/zygotes, while at the 2-cell stage, a strong expression of p53 protein and a larger clear perinuclear area without RAD51 expression were found in TX embryos. In conclusion, we demonstrated for the first time DNA damage in decondensed sperm of zygotes and blastomeres of 2-cell stage embryos sired by TX males, resulting in the activation of DNA repair. Sperm DNA damage could induce the increase in p53 expression and the reduction of RAD51 expression in the TX 2-cell stage embryos. | Animals, Blastomeres, Cell Nucleus, Cricetinae, DNA Damage, Embryo, Mammalian, Female, Genitalia, Male, Male, Mesocricetus, Rad51 Recombinase, Spermatozoa, Tumor Suppressor Protein p53, Zygote | null |
22,705,369 | 2012-12-21 | 2024-04-12 | 1873-4596 | Free radical biology & medicine | Desferrioxamine inhibits protein tyrosine nitration: mechanisms and implications. | Adgent Margaret A, Squadrito Giuseppe L, Ballinger Carol A, Krzywanski David M, Lancaster Jack R, Postlethwait Edward M | eng | P01ES11617 (NIEHS NIH HHS, United States); R01 HL054696 (NHLBI NIH HHS, United States); R01CA131653 (NCI NIH HHS, United States); P01 ES011617 (NIEHS NIH HHS, United States); R01HL54696 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Free Radical Scavengers, Oxidants, Photochemical, Proteins, Siderophores, 3-nitrotyrosine, Tyrosine, Peroxidase, Deferoxamine, Nitrogen Dioxide | IM | 22705369, S0891-5849(12)00338-3, 10.1016/j.freeradbiomed.2012.06.003, PMC3462664, NIHMS399248, 14583336, 12633749, 16236025, 19540354, 9450756, 18771952, 15931769, 9409547, 2695408, 2825650, 14975450, 16052507, 20410298, 18828639, 1474071, 20170094, 18423230, 10652282, 6312841, 2318771, 11179131, 19852560, 8381512, 2820803, 11179096, 16040185, 2840860, 9482858, 12361810, 12134039, 15670581, 19126597, 11848913, 14659696, 7532647, 8529914, 7902116, 9435583, 8023330, 3135299, 4062299, 10339593, 8937563, 16734418, 15657065, 19350240, 8167347, 12033430, 8594022, 12057769, 11328670, 7635352, 11687295, 10777476, 18460345, 6947276, 16627692, 1847381, 12115836, 1757376, 11697033, 3149948 | Tissues are exposed to exogenous and endogenous nitrogen dioxide ((·)NO(2)), which is the terminal agent in protein tyrosine nitration. Besides iron chelation, the hydroxamic acid (HA) desferrioxamine (DFO) shows multiple functionalities including nitration inhibition. To investigate mechanisms whereby DFO affects 3-nitrotyrosine (3-NT) formation, we utilized gas-phase (·)NO(2) exposures, to limit introduction of other reactive species, and a lung surface model wherein red cell membranes (RCM) were immobilized under a defined aqueous film. When RCM were exposed to ()NO(2) covered by +/- DFO: (i) DFO inhibited 3-NT formation more effectively than other HA and non-HA chelators; (ii) 3-NT inhibition occurred at very low[DFO] for prolonged times; and (iii) 3-NT formation was iron independent but inhibition required DFO present. DFO poorly reacted with (·)NO(2) compared to ascorbate, assessed via (·)NO(2) reactive absorption and aqueous-phase oxidation rates, yet limited 3-NT formation at far lower concentrations. DFO also inhibited nitration under aqueous bulk-phase conditions, and inhibited 3-NT generated by active myeloperoxidase "bound" to RCM. Per the above and kinetic analyses suggesting preferential DFO versus (·)NO(2) reaction within membranes, we conclude that DFO inhibits 3-NT formation predominantly by facile repair of the tyrosyl radical intermediate, which prevents (·)NO(2) addition, and thus nitration, and potentially influences biochemical functionalities. | Cell Membrane, Deferoxamine, Erythrocytes, Free Radical Scavengers, Humans, Lung, Nitrogen Dioxide, Oxidants, Photochemical, Peroxidase, Proteins, Siderophores, Tyrosine | null |
22,705,370 | 2012-10-26 | 2023-04-06 | 1097-4164 | Molecular cell | The HARP-like domain-containing protein AH2/ZRANB3 binds to PCNA and participates in cellular response to replication stress. | Yuan Jingsong, Ghosal Gargi, Chen Junjie | eng | P30 CA016672 (NCI NIH HHS, United States); R01 CA092312 (NCI NIH HHS, United States); R01 CA100109 (NCI NIH HHS, United States); CA016672 (NCI NIH HHS, United States); R01 CA089239 (NCI NIH HHS, United States); CA092312 (NCI NIH HHS, United States); CA100109 (NCI NIH HHS, United States); CA089239 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | Proliferating Cell Nuclear Antigen, RNA, Small Interfering, DNA Helicases, ZRANB3 protein, human | IM | 22705370, S1097-2765(12)00441-8, 10.1016/j.molcel.2012.05.025, PMC3601832, NIHMS387803, 19482564, 19793863, 7651832, 1408766, 19793862, 11484057, 10653321, 19793861, 19841479, 12829735, 2066860, 18367739, 4106927, 17512402, 15780937, 19793864, 21525954, 15549093, 12612652, 16377561, 21078962, 12142524, 16862129, 20965415, 16738128, 12453424, 9508763, 18974355, 10857751, 8861913 | Proteins with annealing activity are newly identified ATP-dependent motors that can rewind RPA-coated complementary single-stranded DNA bubbles. AH2 (annealing helicase 2, also named as ZRANB3) is the second protein with annealing activity, the function of which is still unknown. Here, we report that AH2 is recruited to stalled replication forks and that cells depleted of AH2 are hypersensitive to replication stresses. Furthermore, AH2 binds to PCNA, which is crucial for its function at stalled replication forks. Interestingly, we identified a HARP-like (HPL) domain in AH2 that is indispensible for its annealing activity in vitro and its function in vivo. Moreover, searching of HPL domain in SNF2 family of proteins led to the identification of SMARCA1 and RAD54L, both of which possess annealing activity. Thus, this study not only demonstrates the in vivo functions of AH2, but also reveals a common feature of this new subfamily of proteins with annealing activity. | Amino Acid Sequence, Binding Sites, Conserved Sequence, DNA Damage, DNA Helicases, DNA Replication, HEK293 Cells, HeLa Cells, Humans, Molecular Sequence Data, Proliferating Cell Nuclear Antigen, Protein Binding, Protein Structure, Tertiary, RNA, Small Interfering, Stress, Physiological, Structure-Activity Relationship | null |
22,705,371 | 2012-10-26 | 2021-10-21 | 1097-4164 | Molecular cell | A ubiquitin-binding protein, FAAP20, links RNF8-mediated ubiquitination to the Fanconi anemia DNA repair network. | Yan Zhijiang, Guo Rong, Paramasivam Manikandan, Shen Weiping, Ling Chen, Fox David, Wang Yucai, Oostra Anneke B, Kuehl Julia, Lee Duck-Yeon, Takata Minoru, Hoatlin Maureen E, Schindler Detlev, Joenje Hans, de Winter Johan P, Li Lei, Seidman Michael M, Wang Weidong | eng | Z01 AG000688 (Intramural NIH HHS, United States); Z01 AG000688-07 (Intramural NIH HHS, United States); AG000688-07 (NIA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Intramural | DNA-Binding Proteins, FAAP20 protein, human, Fanconi Anemia Complementation Group D2 Protein, Fanconi Anemia Complementation Group Proteins, RNF8 protein, human, Ubiquitin, Green Fluorescent Proteins, Ubiquitin-Protein Ligases, Lysine | IM | 22705371, S1097-2765(12)00442-X, 10.1016/j.molcel.2012.05.026, PMC3398238, NIHMS379928, 21056014, 20603016, 22343915, 19748363, 19797077, 21558560, 18006705, 12724401, 19684342, 19622405, 21464321, 19965384, 19748364, 20347428, 22396592, 18995830, 11239454, 18256278, 21911360, 18001825, 20670894, 17768402, 20551964, 21615334, 19033441, 22266823, 16789823, 20603073, 20603015, 17304240, 18001824, 20671156, 22266820, 20039786, 18077395, 17349954, 19109555, 17296736, 20347429 | The Fanconi anemia (FA) protein network is necessary for repair of DNA interstrand crosslinks (ICLs), but its control mechanism remains unclear. Here we show that the network is regulated by a ubiquitin signaling cascade initiated by RNF8 and its partner, UBC13, and mediated by FAAP20, a component of the FA core complex. FAAP20 preferentially binds the ubiquitin product of RNF8-UBC13, and this ubiquitin-binding activity and RNF8-UBC13 are both required for recruitment of FAAP20 to ICLs. Both RNF8 and FAAP20 are required for recruitment of FA core complex and FANCD2 to ICLs, whereas RNF168 can modulate efficiency of the recruitment. RNF8 and FAAP20 are needed for efficient FANCD2 monoubiquitination, a key step of the FA network; RNF8 and the FA core complex work in the same pathway to promote cellular resistance to ICLs. Thus, the RNF8-FAAP20 ubiquitin cascade is critical for recruiting FA core complex to ICLs and for normal function of the FA network. | Amino Acid Sequence, Animals, Cell Line, Tumor, DNA Repair, DNA-Binding Proteins, Fanconi Anemia, Fanconi Anemia Complementation Group D2 Protein, Fanconi Anemia Complementation Group Proteins, Green Fluorescent Proteins, HEK293 Cells, HeLa Cells, Humans, Immunoblotting, Lysine, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Protein Binding, Protein Structure, Tertiary, RNA Interference, Sequence Homology, Amino Acid, Signal Transduction, Ubiquitin, Ubiquitin-Protein Ligases, Ubiquitination | null |
22,705,375 | 2012-12-10 | 2012-07-30 | 1095-9572 | NeuroImage | Modeling the outcome of structural disconnection on resting-state functional connectivity. | Cabral Joana, Hugues Etienne, Kringelbach Morten L, Deco Gustavo | eng | null | Journal Article | null | IM | 22705375, S1053-8119(12)00584-8, 10.1016/j.neuroimage.2012.06.007 | A growing body of experimental evidence suggests that functional connectivity at rest is shaped by the underlying anatomical structure. Furthermore, the organizational properties of resting-state functional networks are thought to serve as the basis for an optimal cognitive integration. A disconnection at the structural level, as occurring in some brain diseases, would then lead to functional and presumably cognitive impairments. In this work, we propose a computational model to investigate the role of a structural disconnection (encompassing putative local/global and axonal/synaptic mechanisms) on the organizational properties of emergent functional networks. The brain's spontaneous neural activity and the corresponding hemodynamic response were simulated using a large-scale network model, consisting of local neural populations coupled through white matter fibers. For a certain coupling strength, simulations reproduced healthy resting-state functional connectivity with graph properties in the range of the ones reported experimentally. When the structural connectivity is decreased, either globally or locally, the resultant simulated functional connectivity exhibited a network reorganization characterized by an increase in hierarchy, efficiency and robustness, a decrease in small-worldness and clustering and a narrower degree distribution, in the same way as recently reported for schizophrenia patients. Theoretical results indicate that most disconnection-related neuropathologies should induce the same qualitative changes in resting-state brain activity. | Brain, Humans, Models, Neurological, Nerve Net, Neural Pathways, Schizophrenia | null |
22,705,374 | 2012-12-10 | 2012-07-30 | 1095-9572 | NeuroImage | Anatomically-constrained tractography: improved diffusion MRI streamlines tractography through effective use of anatomical information. | Smith Robert E, Tournier Jacques-Donald, Calamante Fernando, Connelly Alan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705374, S1053-8119(12)00582-4, 10.1016/j.neuroimage.2012.06.005 | Diffusion MRI streamlines tractography suffers from a number of inherent limitations, one of which is the accurate determination of when streamlines should be terminated. Use of an accurate streamlines propagation mask from segmentation of an anatomical image confines the streamlines to the volume of the brain white matter, but does not take full advantage of all of the information available from such an image. We present a modular addition to streamlines tractography, which makes more effective use of the information available from anatomical image segmentation, and the known properties of the neuronal axons being reconstructed, to apply biologically realistic priors to the streamlines generated; we refer to this as "Anatomically-Constrained Tractography". Results indicate that some of the known false positives associated with tractography algorithms are prevented, such that the biological accuracy of the reconstructions should be improved, provided that state-of-the-art streamlines tractography methods are used. | Brain, Brain Mapping, Diffusion Tensor Imaging, Humans, Image Interpretation, Computer-Assisted | null |
22,705,373 | 2012-10-26 | 2021-10-21 | 1097-4164 | Molecular cell | Cyclic di-GMP sensing via the innate immune signaling protein STING. | Yin Qian, Tian Yuan, Kabaleeswaran Venkataraman, Jiang Xiaomo, Tu Daqi, Eck Michael J, Chen Zhijian J, Wu Hao | eng | R01 AI045937 (NIAID NIH HHS, United States); R01 AI050872 (NIAID NIH HHS, United States) | Journal Article | Membrane Proteins, STING1 protein, human, bis(3',5')-cyclic diguanylic acid, Cyclic GMP | IM | 22705373, S1097-2765(12)00445-5, 10.1016/j.molcel.2012.05.029, PMC3697849, NIHMS476755, 19433799, 21239155, 12791997, 16681834, 15569936, 18034161, 20124702, 15952891, 22394562, 15170814, 21098106, 10922424, 20197136, 19679088, 18818105, 20890285, 21813776, 19536266, 19158676, 17480182, 20075244, 11130078, 19898478, 15299926, 9757107, 17277122, 19926846, 17618271, 27754618, 21884169, 21892174, 19898477, 19158675, 19776740, 16286919, 8578593, 15572765, 19131592, 20124692, 22579474, 15229469, 18724357, 18559423, 22000019, 21947006 | Detection of foreign materials is the first step of successful immune responses. Stimulator of interferon genes (STING) was shown to directly bind cyclic diguanylate monophosphate (c-di-GMP), a bacterial second messenger, and to elicit strong interferon responses. Here we elucidate the structural features of the cytosolic c-di-GMP binding domain (CBD) of STING and its complex with c-di-GMP. The CBD exhibits an α + β fold and is a dimer in the crystal and in solution. Surprisingly, one c-di-GMP molecule binds to the central crevice of a STING dimer, using a series of stacking and hydrogen bonding interactions. We show that STING is autoinhibited by an intramolecular interaction between the CBD and the C-terminal tail (CTT) and that c-di-GMP releases STING from this autoinhibition by displacing the CTT. The structures provide a remarkable example of pathogen-host interactions in which a unique microbial molecule directly engages the innate immune system. | Amino Acid Sequence, Binding Sites, Cyclic GMP, Dimerization, Humans, Hydrogen Bonding, Immunity, Innate, Membrane Proteins, Molecular Sequence Data, Protein Structure, Tertiary, Signal Transduction | null |
22,705,372 | 2012-10-26 | 2024-03-22 | 1097-4164 | Molecular cell | Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65. | Singh Mahavir, Wang Zhonghua, Koo Bon-Kyung, Patel Anooj, Cascio Duilio, Collins Kathleen, Feigon Juli | eng | R01 GM048123 (NIGMS NIH HHS, United States); R01 GM054198 (NIGMS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | Holoenzymes, Protozoan Proteins, RNA, Protozoan, Ribonucleoproteins, telomerase RNA, RNA, Telomerase | IM | 22705372, S1097-2765(12)00402-9, 10.1016/j.molcel.2012.05.018, PMC3398246, NIHMS378757, 8703216, 20660025, 17322903, 15899850, 15004549, 12741831, 20124702, 16202234, 19246988, 18249148, 16552146, 20057045, 15299374, 20457744, 15254251, 22009680, 16387655, 21212361, 21168327, 19548092, 10742179, 19941821, 19299548, 21653550, 3907856, 16507983, 16713250, 16756500, 16809815, 14976553, 2482765, 20363756, 22015685, 22093366, 12557191, 15318003, 16682180, 12832755, 12842046, 18515081, 22187457, 15696174, 15572765, 9008363, 15082312, 20124692, 12045101, 20713447, 19208095, 19887512, 22315458, 20138158, 14511933, 20106956, 11118222, 19461840, 18547518, 9110970, 15131081, 19287396, 16778765, 7984237, 8602269 | Telomerase is a ribonucleoprotein complex essential for maintenance of telomere DNA at linear chromosome ends. The catalytic core of Tetrahymena telomerase comprises a ternary complex of telomerase RNA (TER), telomerase reverse transcriptase (TERT), and the essential La family protein p65. NMR and crystal structures of p65 C-terminal domain and its complex with stem IV of TER reveal that RNA recognition is achieved by a combination of single- and double-stranded RNA binding, which induces a 105° bend in TER. The domain is a cryptic, atypical RNA recognition motif with a disordered C-terminal extension that forms an α helix in the complex necessary for hierarchical assembly of TERT with p65-TER. This work provides the first structural insight into biogenesis and assembly of TER with a telomerase-specific protein. Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets. | Amino Acid Sequence, Base Sequence, Binding Sites, Crystallography, X-Ray, Electrophoretic Mobility Shift Assay, Holoenzymes, Models, Molecular, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Protozoan Proteins, RNA, RNA, Protozoan, Ribonucleoproteins, Sequence Homology, Amino Acid, Telomerase, Tetrahymena thermophila | null |
22,705,376 | 2012-12-10 | 2012-07-30 | 1095-9572 | NeuroImage | Neural networks related to pro-saccades and anti-saccades revealed by independent component analysis. | Domagalik Aleksandra, Beldzik Ewa, Fafrowicz Magdalena, Oginska Halszka, Marek Tadeusz | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705376, S1053-8119(12)00583-6, 10.1016/j.neuroimage.2012.06.006 | The saccadic eye movement system provides an excellent model for investigating basic cognitive processes and flexible control over behaviour. While the mechanism of pro-saccades (PS) is well known, in the case of the anti-saccade task (AS) it is still not clear which brain regions play a role in the inhibition of reflexive saccade to the target, nor what is the exact mechanism of vector inversion (i.e. orienting in the opposite direction). Independent component analysis (ICA) is one of the methods being used to establish temporally coherent brain regions, i.e. neural networks related to the task. In the present study ICA was applied to fMRI data from PS and AS experiments. The study revealed separate networks responsible for saccade generation into the desired direction, the inhibition of automatic responses, as well as vector inversion. The first function is accomplished by the eye fields network. The inhibition of automatic responses is associated with the executive control network. Vector inversion seems to be accomplished by the network comprising a large set of areas, including intraparietal sulcus, precuneus/posterior cingulate cortices, retrosplenial and parahippocampal. Those regions are associated with the parieto-medial temporal pathway, so far linked only to navigation. These results provide a new insight into understanding of the processes of the inhibition and vector inversion. | Adult, Brain, Brain Mapping, Humans, Image Interpretation, Computer-Assisted, Magnetic Resonance Imaging, Male, Nerve Net, Photic Stimulation, Saccades | null |
22,705,377 | 2012-12-10 | 2012-07-30 | 1095-9572 | NeuroImage | Architectural configuration and microstructural properties of the sacral plexus: a diffusion tensor MRI and fiber tractography study. | van der Jagt Pasquelle K N, Dik Pieter, Froeling Martijn, Kwee Thomas C, Nievelstein Rutger A J, ten Haken Bennie, Leemans Alexander | eng | null | Journal Article | null | IM | 22705377, S1053-8119(12)00578-2, 10.1016/j.neuroimage.2012.06.001 | The ability to investigate microstructural properties of the central nervous system with diffusion tensor imaging (DTI) has been shown in many studies. More recently, DTI is being applied outside the brain showing promising results, for instance, for investigating muscle tissue. In this work, we demonstrate the feasibility of diffusion tensor imaging (DTI) and fiber tractography to study the nerves of the sacral plexus in humans in vivo and to assess the architectural configuration and microstructural properties of these peripheral nerves. For this research goal we optimized the acquisition parameters of a DTI sequence and acquired data from 10 healthy adults and one 12-year patient having spina bifida and neurogenic bladder dysfunction. For the healthy volunteers, we estimated the fractional anisotropy (FA) and mean (MD), axial (AD), and radial diffusivities (RD) of the sacral plexus nerves which may serve as a baseline for future studies. We demonstrated that tractography of the sacral plexus on a 3 Tesla MR scanner is feasible, giving 3D insight in the general anatomy and organization of the nerves L4 to S3. In addition, branches to the pudendal nerve were also found in 4 volunteers. There were no significant differences in any of the estimated diffusion measures between the right and left sided nerves or between the nerves L4 to S3 on an intra-subject basis. Furthermore, clinical feasibility of DTI and tractography in a child having spina bifida and neurogenic bladder dysfunction is demonstrated. The architectural configuration of the child's sacral plexus was comparable with the healthy volunteers and no significant disrupted nerve fibers were observed. However, there are strong indications that abnormal diffusion characteristics are present at the level of the neural tube defect due to incomplete segments of the nerves that are close to the vertebrae. These findings are encouraging for using DTI as a means to investigate changes in microstructural properties of the nerves of the sacral plexus. Moreover, this new methodology may provide a new avenue to a better analysis and diagnosis of neurogenic bladder dysfunctions. | Adult, Diffusion Magnetic Resonance Imaging, Diffusion Tensor Imaging, Female, Humans, Image Interpretation, Computer-Assisted, Lumbosacral Plexus, Male, Young Adult | null |
22,705,378 | 2012-11-07 | 2017-11-16 | 1096-0309 | Analytical biochemistry | The Dac-tag, an affinity tag based on penicillin-binding protein 5. | Lee David Wei, Peggie Mark, Deak Maria, Toth Rachel, Gage Zoe Olivia, Wood Nicola, Schilde Christina, Kurz Thimo, Knebel Axel | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Buffers, Escherichia coli Proteins, PBP5 protein, E coli, Penicillin-Binding Proteins, Recombinant Fusion Proteins, Green Fluorescent Proteins | IM | 22705378, S0003-2697(12)00312-0, 10.1016/j.ab.2012.06.007 | Penicillin-binding protein 5 (PBP5), a product of the Escherichia coli gene dacA, possesses some β-lactamase activity. On binding to penicillin or related antibiotics via an ester bond, it deacylates and destroys them functionally by opening the β-lactam ring. This process takes several minutes. We exploited this process and showed that a fragment of PBP5 can be used as a reversible and monomeric affinity tag. At ambient temperature (e.g., 22°C), a PBP5 fragment binds rapidly and specifically to ampicillin Sepharose. Release can be facilitated either by eluting with 10mM ampicillin or in a ligand-free manner by incubation in the cold (1-10°C) in the presence of 5% glycerol. The "Dac-tag", named with reference to the gene dacA, allows the isolation of remarkably pure fusion protein from a wide variety of expression systems, including (in particular) eukaryotic expression systems. | Animals, Buffers, Chromatography, Affinity, Dictyostelium, Escherichia coli Proteins, Green Fluorescent Proteins, HEK293 Cells, Humans, Insecta, Penicillin-Binding Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae | null |
22,705,380 | 2013-01-28 | 2022-04-09 | 1873-1570 | Resuscitation | Characteristics of lifesaving from drowning as reported by the Swedish Fire and Rescue Services 1996-2010. | Claesson Andreas, Lindqvist Jonny, Ortenwall Per, Herlitz Johan | eng | null | Journal Article | null | IM | 22705380, S0300-9572(12)00303-6, 10.1016/j.resuscitation.2012.05.025 | We aimed to describe characteristics associated with rescue from drowning as reported by the Swedish Fire and Rescue Services (SFARS) and their association with survival from the Out of Hospital Cardiac Arrest (OHCA) registry. | Adult, Aged, Emergency Treatment, Female, Firefighters, Humans, Male, Middle Aged, Near Drowning, Retrospective Studies, Sweden, Young Adult | null |
22,705,379 | 2012-11-06 | 2012-11-15 | 1872-7786 | Chemico-biological interactions | Pimaric acid from Aralia cordata has an inhibitory effect on TNF-α-induced MMP-9 production and HASMC migration via down-regulated NF-κB and AP-1. | Suh Seok-Jong, Kwak Choong-Hwan, Chung Tae-Wook, Park Soon Jin, Cheeeei Meijuan, Park Sung-Soo, Seo Chang-Seob, Son Jong-Keun, Chang Young-Chae, Park Young-Guk, Lee Young-Choon, Chang Hyeun Wook, Kim Cheorl-Ho | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Inflammatory Agents, Diterpenes, NF-kappa B, Transcription Factor AP-1, Tumor Necrosis Factor-alpha, pimaric acid, Matrix Metalloproteinase 9 | IM | 22705379, S0009-2797(12)00102-0, 10.1016/j.cbi.2012.06.003 | Many studies have indicated that activation of matrix metalloproteinase (MMP)-9 and smooth muscle cell (SMC) migration are involved in neointimal formation and atherosclerosis. In this study, we revealed that pimaric acid (PiMA) purified from Aralia cordata had an inhibitory effect on MMP-9 production and migration of human aortic smooth muscle cells (HASMCs) induced by tumor necrosis factor (TNF)-α. Down-regulated MMP-9 mRNA transcription was detected in PiMA-treated cells using RT-PCR and the luciferase-tagged MMP-9 promoter assay. Results of an electrophoretic mobility shift assay indicated that PiMA-treated HASMCs showed decreased binding activity of nuclear factor (NF)-κB and activator protein-1 transcription factors. A Western-blot analysis using nuclear extract demonstrated that PiMA reduced the levels of NF-κB p65, c-Fos, p-c-Jun, Jun-D, and p-ATF2 proteins in the nucleus. In addition, TNF-α stimulated mitogen activated protein kinase (MAPK) containing extracellular signal regulated kinase 1 and 2, p38, and c-Jun N-terminal kinase was inhibited by PiMA. Using the Transwell system, we found that PiMA inhibited TNF-α stimulated HASMC migration/invasion in a dose-dependent manner. To confirm whether MAPK mediated MMP-9 expression, we used MAPK inhibitors including U0126, SB253580, and SP600125 and found that those inhibitors reduced MMP-9 expression and HASMC migration/invasion. These results suggest that PiMA has potent anti-atherosclerotic activity with inhibitory action on MMP-9 production and cell migration in TNF-α-induced HASMCs. | Anti-Inflammatory Agents, Aorta, Aralia, Cell Line, Cell Movement, Diterpenes, Down-Regulation, Humans, MAP Kinase Signaling System, Matrix Metalloproteinase 9, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, NF-kappa B, Phosphorylation, Transcription Factor AP-1, Tumor Necrosis Factor-alpha | null |
22,705,381 | 2012-11-26 | 2012-07-24 | 1872-8359 | Journal of microbiological methods | Development of species-, strain- and antibiotic biosynthesis-specific quantitative PCR assays for Pantoea agglomerans as tools for biocontrol monitoring. | Braun-Kiewnick Andrea, Lehmann Andreas, Rezzonico Fabio, Wend Chris, Smits Theo H M, Duffy Brion | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Biological Control Agents, DNA Primers, Glycopeptides, pantocin A | IM | 22705381, S0167-7012(12)00217-5, 10.1016/j.mimet.2012.06.004 | Pantoea agglomerans is a cosmopolitan plant epiphytic bacterium that includes some of the most effective biological antagonists against the fire blight pathogen Erwinia amylovora, a major threat to pome fruit production worldwide. Strain E325 is commercially available as Bloomtime Biological™ in the USA and Canada. New quantitative PCR (qPCR) assays were developed for species- and strain -specific detection in the environment, and for detection of indigenous strains carrying the biocontrol antibacterial peptide biosynthesis gene paaA. The qPCR assays were highly specific, efficient and sensitive, detecting fewer than three cells per reaction or 700 colony forming units per flower, respectively. The qPCR assays were tested on field samples, giving first indications to the incidence of P. agglomerans E325 related strains, total P. agglomerans and pantocin A producing bacteria in commercial orchards. These assays will facilitate monitoring the environmental behavior of biocontrol P. agglomerans after orchard application for disease protection, proprietary strain-tracking, and streamlined screening for discovery of new biocontrol strains. | Anti-Bacterial Agents, Biological Control Agents, DNA Primers, Flowers, Glycopeptides, Limit of Detection, Malus, Molecular Typing, Pantoea, Real-Time Polymerase Chain Reaction, Species Specificity | null |
22,705,383 | 2013-01-07 | 2018-12-01 | 1873-7560 | European urology | Robot-assisted radical prostatectomy - fake innovation or the real deal? | Albertsen Peter C | eng | null | Editorial, Comment | null | IM | 22705383, S0302-2838(12)00693-8, 10.1016/j.eururo.2012.06.002 | null | Benchmarking, Erectile Dysfunction, Humans, Laparoscopy, Male, Penile Erection, Prostatectomy, Prostatic Neoplasms, Robotics, Surgery, Computer-Assisted, Urinary Bladder, Urinary Incontinence | null |
22,705,382 | 2013-03-06 | 2022-04-10 | 1873-7560 | European urology | Standardized analysis of frequency and severity of complications after robot-assisted radical cystectomy. | Yuh Bertram E, Nazmy Michael, Ruel Nora H, Jankowski Jason T, Menchaca Anita R, Torrey Robert R, Linehan Jennifer A, Lau Clayton S, Chan Kevin G, Wilson Timothy G | eng | null | Journal Article | null | IM | 22705382, S0302-2838(12)00708-7, 10.1016/j.eururo.2012.06.007 | Comprehensive and standardized reporting of adverse events after robot-assisted radical cystectomy (RARC) and urinary diversion for bladder cancer is necessary to evaluate the magnitude of morbidity for this complex operation. | Aged, Blood Transfusion, Chi-Square Distribution, Comorbidity, Cystectomy, Female, Humans, Incidence, Logistic Models, Lymph Node Excision, Male, Middle Aged, Multivariate Analysis, Postoperative Complications, Research Design, Retrospective Studies, Risk Assessment, Risk Factors, Robotics, Severity of Illness Index, Surgery, Computer-Assisted, Time Factors, Treatment Outcome, Urinary Bladder Neoplasms, Urinary Diversion | null |
22,705,384 | 2012-11-05 | 2018-12-01 | 1090-2430 | Experimental neurology | Alteration in the progression of dopamine neuron degeneration: may caffeine offer new perspective? | Morelli Micaela, Frau Lucia, Simola Nicola | eng | null | Journal Article, Comment | Caffeine | IM | 22705384, S0014-4886(12)00234-8, 10.1016/j.expneurol.2012.05.023 | null | Animals, Caffeine, Dopaminergic Neurons, Male, Nerve Degeneration, Parkinson Disease, Substantia Nigra | null |
22,705,385 | 2012-11-05 | 2018-12-01 | 1090-2430 | Experimental neurology | Neural development, a risky period. | Sladek John R | eng | null | Journal Article, Comment | Dopamine | IM | 22705385, S0014-4886(12)00235-X, 10.1016/j.expneurol.2012.05.024 | null | Animals, Corpus Striatum, Dopamine, MPTP Poisoning, Substantia Nigra | null |
22,705,386 | 2013-03-05 | 2012-10-12 | 1638-6183 | Biochimie | A pipeline for the identification and characterization of chromatin modifications derived from ChIP-Seq datasets. | Kaspi Antony, Ziemann Mark, Rafehi Haloom, Lazarus Ross, El-Osta Assam | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Chromatin | IM | 22705386, S0300-9084(12)00236-2, 10.1016/j.biochi.2012.06.002 | The advent of massive parallel sequencing of immunopurified chromatin and its determinants has provided new avenues for researchers to map epigenome-wide changes and there is tremendous interest to uncover regulatory signatures to understand fundamental questions associated with chromatin structure and function. Indeed, the rapid development of large genome annotation projects has seen a resurgence in chromatin immunoprecipitation (ChIP) based protocols which are used to distinguish protein interactions coupled with large scale sequencing (Seq) to precisely map epigenome-wide interactions. Despite some of the great advances in our understanding of chromatin modifying complexes and their determinants, the development of ChIP-Seq technologies also pose specific demands on the integration of data for visualization, manipulation and analysis. In this article we discuss some of the considerations for experimental design planning, quality control, and bioinformatic analysis. The key aspects of post sequencing analysis are the identification of regions of interest, differentiation between biological conditions and the characterization of sequence differences for chromatin modifications. We provide an overview of best-practise approaches with background information and considerations of integrative analysis from ChIP-Seq experiments. | Animals, Chromatin, Chromatin Immunoprecipitation, Computational Biology, Humans, Mice, Molecular Sequence Annotation, Sequence Analysis, DNA | null |
22,705,387 | 2013-05-23 | 2022-12-07 | 1638-6183 | Biochimie | Jasmonates in flower and seed development. | Wasternack Claus, Forner Susanne, Strnad Miroslav, Hause Bettina | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Cyclopentanes, Fatty Acids, Unsaturated, Oxylipins, Plant Growth Regulators, jasmonoyl-isoleucine, Isoleucine, 12-oxophytodienoic acid, jasmonic acid | IM | 22705387, S0300-9084(12)00239-8, 10.1016/j.biochi.2012.06.005 | Jasmonates are ubiquitously occurring lipid-derived signaling compounds active in plant development and plant responses to biotic and abiotic stresses. Upon environmental stimuli jasmonates are formed and accumulate transiently. During flower and seed development, jasmonic acid (JA) and a remarkable number of different metabolites accumulate organ- and tissue specifically. The accumulation is accompanied with expression of jasmonate-inducible genes. Among these genes there are defense genes and developmentally regulated genes. The profile of jasmonate compounds in flowers and seeds covers active signaling molecules such as JA, its precursor 12-oxophytodienoic acid (OPDA) and amino acid conjugates such as JA-Ile, but also inactive signaling molecules occur such as 12-hydroxy-JA and its sulfated derivative. These latter compounds can occur at several orders of magnitude higher level than JA. Metabolic conversion of JA and JA-Ile to hydroxylated compounds seems to inactivate JA signaling, but also specific functions of jasmonates in flower and seed development were detected. In tomato OPDA is involved in embryo development. Occurrence of jasmonates, expression of JA-inducible genes and JA-dependent processes in flower and seed development will be discussed. | Cyclopentanes, Fatty Acids, Unsaturated, Flowers, Gene Expression Regulation, Plant, Isoleucine, Solanum lycopersicum, Oxylipins, Plant Growth Regulators, Plant Leaves, Seeds, Signal Transduction, Stress, Physiological | null |
22,705,388 | 2013-02-05 | 2021-10-21 | 1878-5832 | Drug discovery today | Does transbilayer diffusion have a role in membrane transport of drugs? | Balaz Stefan | eng | P41 RR006009 (NCRR NIH HHS, United States); R01 GM080508 (NIGMS NIH HHS, United States) | Journal Article, Review | Lipid Bilayers, Pharmaceutical Preparations | IM | 22705388, S1359-6446(12)00196-1, 10.1016/j.drudis.2012.06.003, PMC3459300, NIHMS387681, 13417090, 15950595, 1334228, 21800872, 16250332, 22468611, 3905031, 8703982, 9874698, 7381427, 17384063, 12598370, 9271493, 7547874, 718892, 16859285, 14367406, 21624498, 857018, 10903312, 20671764, 836824, 1784187, 9449332, 14476933, 3067757, 19200003, 12551728, 6525345, 6478029, 1260054, 18504571, 9348427, 21262843, 11334238, 10667915, 15041656, 19265398, 2427233, 9778346, 9512032, 16460120, 8770210, 10878238, 18309312, 3207838 | The existing consensus on coexistence of transbilayer diffusion and carrier-mediated transport as two main mechanisms for drugs crossing biological membranes was recently challenged by a systems biology group. Their transporters-only hypothesis is examined in this article using published experimental evidence. The main focus is on the key claim of their hypothesis, stating that 'the drug molecules cross pure phospholipid bilayers through transient pores that cannot form in the bilayers of cell membranes, and thus transbilayer drug transport does not exist in cells'. The analysis shows that the prior consensus remains a valid scientific view of the membrane transport of drugs. | Animals, Biological Transport, Cell Membrane, Diffusion, Humans, Lipid Bilayers, Pharmaceutical Preparations | null |
22,705,389 | 2012-11-12 | 2021-10-21 | 1879-1646 | Journal of health economics | The value of medical and pharmaceutical interventions for reducing obesity. | Michaud Pierre-Carl, Goldman Dana P, Lakdawalla Darius N, Zheng Yuhui, Gailey Adam H | eng | P30 AG024968 (NIA NIH HHS, United States); P30 AG043073 (NIA NIH HHS, United States); R01 AG030824 (NIA NIH HHS, United States); R01AG030824 (NIA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | null | null | 22705389, S0167-6296(12)00054-9, 10.1016/j.jhealeco.2012.04.006, PMC3600147, NIHMS388145, 12427499, 15840860, 18212316, 9481598, 11191537, 16232005, 15616203, 9321534, 22253363, 19748839, 12000688, 18006966, 17717337, 10167341, 19762651, 10527295, 19726018, 18778174, 18513809 | This paper attempts to quantify the social, private, and public-finance values of reducing obesity through pharmaceutical and medical interventions. We find that the total social value of bariatric surgery is large for treated patients, with incremental social cost-effectiveness ratios typically under $10,000 per life-year saved. On the other hand, pharmaceutical interventions against obesity yield much less social value with incremental social cost-effectiveness ratios around $50,000. Our approach accounts for: competing risks to life expectancy; health care costs; and a variety of non-medical economic consequences (pensions, disability insurance, taxes, and earnings), which account for 20% of the total social cost of these treatments. On balance, bariatric surgery generates substantial private value for those treated, in the form of health and other economic consequences. The net public fiscal effects are modest, primarily because the size of the population eligible for treatment is small. The net social effect is large once improvements in life expectancy are taken into account. | Bariatric Surgery, Cost-Benefit Analysis, Drug Therapy, Humans, Models, Econometric, Obesity, Treatment Outcome | null |
22,705,391 | 2012-11-21 | 2013-11-21 | 1873-3514 | Neuropsychologia | Motor cortex excitability is tightly coupled to observed movements. | Sartori Luisa, Bucchioni Giulia, Castiello Umberto | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705391, S0028-3932(12)00240-0, 10.1016/j.neuropsychologia.2012.06.002 | Although facilitation of the corticospinal (CS) system during action observation is a widely accepted phenomenon, it is still controversial if facilitation reflects the replica of observed movement kinematics or the tension to achieve a particular goal. In this study, transcranial magnetic stimulation (TMS)-induced motor evoked potentials (MEPs) were recorded from the abductor digiti minimi (ADM) and the first dorsal interosseus (FDI) muscles while participant-volunteers observed a model grasping a small target eliciting a precision grip or a large target eliciting a whole hand grasp directed toward an isolated object or flanked by different sized objects (i.e., distractor). A detailed movement analysis revealed that the model's kinematics were influenced by the distractor's size. Video clips filming the scene were edited in such a way that the distractor was removed from the scene. Participant-volunteers were asked to observe actions characterized by the same goal but performed using different kinematical patterns. Although the differences in movement kinematics were not noticed by the participant-volunteers, they nonetheless elicited distinct configurations of corticospinal activation. Detailed motor matching seems to recruit the same muscles in the onlooker as in the person actually carrying out the action during observation of grasping actions. These effects appear to be elicited by very subtle, imperceptible aspects of observed actions pointing to a finely tuned mechanism that specifically encodes body parts. | Adult, Biomechanical Phenomena, Data Interpretation, Statistical, Evoked Potentials, Motor, Female, Fingers, Hand Strength, Humans, Male, Motion Perception, Motor Cortex, Movement, Photic Stimulation, Psychomotor Performance, Transcranial Magnetic Stimulation, Visual Perception, Young Adult | null |
22,705,390 | 2012-11-12 | 2017-11-16 | 1879-1646 | Journal of health economics | Does staying in school (and not working) prevent teen smoking and drinking? | Jensen Robert, Lleras-Muney Adriana | eng | null | Journal Article, Randomized Controlled Trial | null | null | 22705390, S0167-6296(12)00058-6, 10.1016/j.jhealeco.2012.05.004 | Previous work suggests but cannot prove that education improves health behaviors. We exploit a randomized intervention that increased schooling (and reduced working) among male students in the Dominican Republic, by providing information on the returns to schooling. We find that treated youths were much less likely to smoke at age 18 and had delayed onset of daily or regular drinking. The effects appear to be due to changes in peer networks and disposable income. We find no evidence of a direct impact of schooling on rates of time preference, attitudes towards risk or perceptions that drinking or smoking are harmful to health, though our measures of these factors are more limited. | Adolescent, Alcohol Drinking, Dominican Republic, Educational Status, Employment, Humans, Male, Smoking Prevention, Students | null |
22,705,392 | 2013-01-02 | 2012-08-20 | 1873-5177 | Pharmacology, biochemistry, and behavior | The novel adenosine A(2A) antagonist prodrug MSX-4 is effective in animal models related to motivational and motor functions. | Santerre Jessica L, Nunes Eric J, Kovner Rotem, Leser Chelsea E, Randall Patrick A, Collins-Praino Lyndsey E, Lopez Cruz Laura, Correa Merce, Baqi Younis, Müller Christa E, Salamone John D | eng | MH078023 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Adenosine A2 Receptor Antagonists, Prodrugs | IM | 22705392, S0091-3057(12)00164-5, 10.1016/j.pbb.2012.06.009 | Adenosine A(2A) and dopamine D2 receptors interact to regulate diverse aspects of ventral and dorsal striatal functions related to motivational and motor processes, and it has been suggested that adenosine A(2A) antagonists could be useful for the treatment of depression, parkinsonism and other disorders. The present experiments were performed to characterize the effects of MSX-4, which is an amino acid ester prodrug of the potent and selective adenosine A(2A) receptor antagonist MSX-2, by assessing its ability to reverse pharmacologically induced motivational and motor impairments. In the first group of studies, MSX-4 reversed the effects of the D2 antagonist eticlopride on a concurrent lever pressing/chow feeding task that is used as a measure of effort-related choice behavior. MSX-4 was less potent after intraperitoneal administration than the comparison compound, MSX-3, though both were equally efficacious. With this task, MSX-4 was orally active in the same dose range as MSX-3. MSX-4 also reversed the locomotor suppression induced by eticlopride in the open field, but did not induce anxiogenic effects as measured by the relative amount of interior activity. Behaviorally active doses of MSX-4 also attenuated the increase in c-Fos and pDARPP-32(Thr34) expression in nucleus accumbens core that was induced by injections of eticlopride. In addition, MSX-4 suppressed the oral tremor induced by the anticholinesterase galantamine, which is consistent with an antiparkinsonian profile. These actions of MSX-4 indicate that this compound could have potential utility as a treatment for parkinsonism, as well as some of the motivational symptoms of depression and other disorders. | Adenosine A2 Receptor Antagonists, Animals, Locomotion, Male, Models, Animal, Motivation, Prodrugs, Rats, Rats, Sprague-Dawley | null |
22,705,393 | 2012-10-09 | 2019-04-04 | 1878-1551 | Developmental cell | Identification and regulation of a molecular module for bleb-based cell motility. | Goudarzi Mehdi, Banisch Torsten U, Mobin Mehrpouya B, Maghelli Nicola, Tarbashevich Katsiaryna, Strate Ina, van den Berg Jana, Blaser Heiko, Bandemer Sabine, Paluch Ewa, Bakkers Jeroen, Tolić-Nørrelykke Iva M, Raz Erez | eng | null | Journal Article, Research Support, Non-U.S. Gov't | MicroRNAs, Mirn430 microRNA, zebrafish, RNA-Binding Proteins, Zebrafish Proteins, dnd1 protein, zebrafish, Myosins | IM | 22705393, S1534-5807(12)00234-1, 10.1016/j.devcel.2012.05.007 | Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment. | Animals, Animals, Genetically Modified, Base Sequence, Cell Adhesion, Cell Movement, Cell Polarity, Germ Cells, Homeostasis, Hydrostatic Pressure, MicroRNAs, Molecular Sequence Data, Myosins, RNA-Binding Proteins, Zebrafish, Zebrafish Proteins | null |
22,705,395 | 2013-01-16 | 2012-09-03 | 1769-7123 | Research in microbiology | Synergistic effect of antimicrobial peptide arenicin-1 in combination with antibiotics against pathogenic bacteria. | Choi Hyemin, Lee Dong Gun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Helminth Proteins, arenicin-1, Arenicola marina, Hydroxyl Radical | IM | 22705395, S0923-2508(12)00080-0, 10.1016/j.resmic.2012.06.001 | Arenicin-1, a 21-mer antimicrobial peptide, has been known to exert broad bactericidal activity. In this study, the combination effect of arenicin-1 with conventional antibiotics was investigated and all combinations showed synergistic effects against bacterial strains (fractional inhibitory concentration index ≤ 0.75). In an assay using fluorescent dye 3'-(p-hydroxyphenyl) fluorescein (HPF) and hydroxyl radical scavenger thiourea, we demonstrated that combined treatments of arenicin-1 and antibiotics caused synergistic effects by producing hydroxyl radicals, particularly in bacterial strains treated with the peptide and ampicillin. The oxidative stress induced by arenicin-1 was stimulated by transient depletion of NADH. Flow cytometric analysis with propidium iodide (PI) indicated that MIC of arenicin-1 for combination continued to increase the permeability of the bacterial cytoplasmic membrane, enhancing the entry of erythromycin and chloramphenicol which act as protein synthesis inhibitors. Therefore, arenicin-1 synergizes with antibiotics by means of hydroxyl radical formation or membrane-active mechanisms. This combination therapy allows the use of lower concentrations of arenicin-1 and restores the effectiveness of antibiotics. | Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Cell Membrane Permeability, Drug Synergism, Gram-Negative Bacteria, Gram-Positive Bacteria, Helminth Proteins, Hydroxyl Radical, Microbial Sensitivity Tests, Oxidative Stress | null |
22,705,394 | 2012-10-09 | 2021-12-03 | 1878-1551 | Developmental cell | A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery. | Burgo Andrea, Proux-Gillardeaux Véronique, Sotirakis Emmanuel, Bun Philippe, Casano Alessandra, Verraes Agathe, Liem Ronald K H, Formstecher Etienne, Coppey-Moisan Maïté, Galli Thierry | eng | null | Journal Article, Research Support, Non-U.S. Gov't | KIF5A protein, human, R-SNARE Proteins, RNA, Small Interfering, Tubulin Modulators, VAMP7 protein, human, Kinesins, Nocodazole | IM | 22705394, S1534-5807(12)00196-7, 10.1016/j.devcel.2012.04.019 | The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface. | Animals, COS Cells, Chlorocebus aethiops, Golgi Apparatus, Growth Cones, HeLa Cells, Humans, Kinesins, Nocodazole, Protein Transport, R-SNARE Proteins, RNA, Small Interfering, Tubulin Modulators | null |
22,705,396 | 2013-02-11 | 2018-12-01 | 1873-7064 | Neuropharmacology | The potential of nicotinic enhancement of cognitive remediation training in schizophrenia. | Hahn Britta, Gold James M, Buchanan Robert W | eng | null | Journal Article | Nerve Tissue Proteins, Nicotinic Agonists, Nootropic Agents, Receptors, Nicotinic | IM | 22705396, S0028-3908(12)00258-4, 10.1016/j.neuropharm.2012.05.050 | Cognitive deficits in schizophrenia are critically important predictors of long-term psychosocial outcome and are not significantly ameliorated by currently available medications. Cognitive remediation training has shown promise for alleviating cognitive symptoms of schizophrenia, but the clinical significance has often been limited by small effect sizes. Approaches that achieve larger improvement involve time requirements that can be cost-prohibitive within the current clinical care system. This mini-review evaluates the theoretical potential of a pharmacological enhancement strategy of cognitive remediation training with nicotinic acetylcholine receptor (nAChR) agonists. nAChR agonists can facilitate sensory processing, alertness, attention, learning and memory. While these effects may be too subtle and short-lasting to be of clinical relevance as a primary treatment of cognitive deficits, they constitute an ideal effects profile for enhancing training benefits. Several mechanisms are described through which repeated coupling of cognitive training challenges with nAChR stimulation may enhance and accelerate cognitive remediation training effects, advancing such interventions into more effective and practicable treatments of some of the most debilitating symptoms of schizophrenia. This article is part of a Special Issue entitled 'Cognitive Enhancers'. | Animals, Brain, Cognition Disorders, Cognitive Behavioral Therapy, Combined Modality Therapy, Humans, Molecular Targeted Therapy, Nerve Tissue Proteins, Neurons, Nicotinic Agonists, Nootropic Agents, Receptors, Nicotinic, Schizophrenia | null |
22,705,397 | 2014-01-13 | 2013-06-03 | 1724-191X | Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB) | Novel radio-chromic solution dosimeter for radiotherapy treatment planning. | Rabaeh Khalid A, Moussa Akram A, Basfar Ahmed A, Msalam Rashed I | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Coloring Agents, Solutions, Nitroblue Tetrazolium | IM | 22705397, S1120-1797(12)00044-0, 10.1016/j.ejmp.2012.05.006 | Nitro blue tetrazolium (NBT) solution dosimeters were prepared and investigated based on radiation-induced reduction of NBT(2+). NBT solution dosimeters containing different concentrations of NBT dye from 1 to 5 mM were prepared in a solution of ethanol. The dosimeters were irradiated with 6 MV X-ray beam at doses up to 30 Gy. The dose sensitivity of NBT solution increases strongly with increase of concentrations of NBT dye. The dose response of NBT dosimeters increases remarkably by addition of various concentrations of sodium formate (0.5, 2.5 and 5 mM). It becomes more remarkable with increasing pH value of NBT-sodium formate dosimeters. The sensitivity of the solution increased fairly with increase of irradiation temperature, therefore, the response of the solutions has to be corrected under actual processing conditions. The stability of solution dosimeters after irradiation was very high up to 30 days. | Coloring Agents, Hydrogen-Ion Concentration, Nitroblue Tetrazolium, Radiometry, Radiotherapy Dosage, Radiotherapy Planning, Computer-Assisted, Solutions, Temperature, X-Rays | null |
22,705,398 | 2012-10-16 | 2013-11-21 | 1096-0384 | Archives of biochemistry and biophysics | Stimulation of KatG catalase activity by peroxidatic electron donors. | Ndontsa Elizabeth N, Moore Robert L, Goodwin Douglas C | eng | null | Journal Article, Research Support, U.S. Gov't, Non-P.H.S. | Escherichia coli Proteins, Peroxides, Hydrogen Peroxide, Iron, Peroxidases, Catalase, katG protein, E coli, Oxygen | IM | 22705398, S0003-9861(12)00248-2, 10.1016/j.abb.2012.06.003 | Catalase-peroxidases (KatGs) use a peroxidase scaffold to support robust catalase activity, an ability no other member of its superfamily possesses. Because catalase turnover requires H(2)O(2) oxidation, whereas peroxidase turnover requires oxidation of an exogenous electron donor, it has been anticipated that the latter should inhibit catalase activity. To the contrary, we report peroxidatic electron donors stimulated catalase activity up to 14-fold, particularly under conditions favorable to peroxidase activity (i.e., acidic pH and low H(2)O(2) concentrations). We observed a "low-" and "high-K(M)" component for catalase activity at pH 5.0. Electron donors increased the apparent k(cat) for the "low-K(M)" component. During stimulated catalase activity, less than 0.008 equivalents of oxidized donor accumulated for every H(2)O(2) consumed. Several classical peroxidatic electron donors were effective stimulators of catalase activity, but pyrogallol and ascorbate showed little effect. Stopped-flow evaluation showed that a Fe(III)-O(2)(·-)-like intermediate dominated during donor-stimulated catalatic turnover, and this intermediate converted directly to the ferric state upon depletion of H(2)O(2). In this respect, the Fe(III)-O(2)(·-) -like species was more prominent and persistent than in the absence of the donor. These results point toward a much more central role for peroxidase substrates in the unusual catalase mechanism of KatG. | Catalase, Dose-Response Relationship, Drug, Electrons, Escherichia coli, Escherichia coli Proteins, Hydrogen Peroxide, Hydrogen-Ion Concentration, Iron, Kinetics, Oxygen, Peroxidases, Peroxides, Ultraviolet Rays | null |
22,705,399 | 2012-11-26 | 2012-08-07 | 1095-9513 | Molecular phylogenetics and evolution | A molecular phylogeny of the Lepidozia generic complex supports re-circumscription of the Lepidozioideae. | Cooper Endymion D, Henwood Murray J, Brown Elizabeth A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Chloroplast, DNA, Mitochondrial, DNA, Plant, DNA, Ribosomal Spacer | IM | 22705399, S1055-7903(12)00174-1, 10.1016/j.ympev.2012.05.008 | Five molecular markers (chloroplast rbcL and trnL-trnF, mitochondrial nad5-nad4, and nuclear ITS1 and ITS2) were used to investigate membership of the Lepidoziaceae, subfamily Lepidozioideae and relationships between its constituent species. The Lepidozioideae (comprising Lepidozia, Telaranea, Kurzia, Sprucella, Psiloclada) are polyphyletic as are two of its five constituent genera (Telaranea and Kurzia). We find strong support for a monophyletic lineage comprising Lepidozia, Sprucella (nested within Lepidozia), and part of Telaranea. Within this lineage we find partial support for four main clades. Three clades of Telaranea species form consecutive sister relationships to a monophyletic Lepidozia. Relationships within Lepidozia are incompletely resolved. We provide a re-circumscription of the Lepidozioideae that excludes Kurzia, Psiloclada and elements of Telaranea. | Australia, Bayes Theorem, Biological Evolution, Cell Nucleus, DNA, Chloroplast, DNA, Mitochondrial, DNA, Plant, DNA, Ribosomal Spacer, Hepatophyta, New Zealand, Phylogeny, Sequence Analysis, DNA | null |
22,705,400 | 2012-11-28 | 2013-11-21 | 1873-3492 | Clinica chimica acta; international journal of clinical chemistry | Mucin genes in gallstone disease. | Chuang Shih-Chang, Hsi Edward, Lee King-Teh | eng | null | Journal Article, Review | Bile Acids and Salts, Mucins, Protein Isoforms, Cholesterol | IM | 22705400, S0009-8981(12)00317-8, 10.1016/j.cca.2012.06.015 | Gallstone disease is a complex disorder that can be caused by environmental influences, common genetic factors and their interactions. Three major pathogenic abnormalities are considered to involve in gallstone formation: cholesterol supersaturation in bile, precipitation and nucleation of excess cholesterol, and gallbladder hypomotility, while, mucin takes part in the cholesterol nucleation process. Up to date, more than 20 mucin genes have been reported, 9 of them are identified at the mRNA and/or protein level in native gallbladder and its associated diseases. In the gallbladder, mucin is essential for best protection against detergent effect of high concentration of bile acids. Over the past decade, the properties, expressions and functions of the gallbladder mucins are delineated in animal and human studies. Alteration expressions of mucins are thought to response during the pathogenesis of gallstone formation. Moreover, recent genetic association study demonstrated mucin gene polymorphisms may also influence susceptibility to gallstone disease. This review is not to provide a complete coverage of all the aspects of mucin glycoproteins, but focus on the role and expression of mucins involve in the regulation of cholelithogenesis. | Bile, Bile Acids and Salts, Cholesterol, Gallbladder, Gallstones, Gene Expression, Genetic Predisposition to Disease, Humans, Mucins, Multigene Family, Polymorphism, Genetic, Protein Isoforms | null |
22,705,401 | 2012-11-28 | 2021-12-03 | 1873-3492 | Clinica chimica acta; international journal of clinical chemistry | A novel multiplex analysis of filaggrin polymorphisms: a universally applicable method for genotyping. | Meldgaard Michael, Szecsi Pal B, Carlsen Berit C, Thyssen Jacob P, Johansen Jeanne D, Menné Torkil, Stender Steen | eng | null | Journal Article | DNA Primers, DNA, Single-Stranded, FLG protein, human, Filaggrin Proteins, Intermediate Filament Proteins, Protein Isoforms | IM | 22705401, S0009-8981(12)00316-6, 10.1016/j.cca.2012.06.014 | The filaggrin protein is expressed as profilaggrin mainly in stratum granulosum cells of the epidermis. The profilaggrin gene codes for 10-12 filaggrin repeats. The filaggrin protein is important for skin barrier function. Filaggrin deficiency due to functional null-polymorphisms affects 8-10% of the people in Northern Europe and is a strong risk factor for several diseases. Here, we describe a novel method for efficient, multiplexed genotyping of variations in the profilaggrin gene. | Alleles, Biotinylation, DNA Primers, DNA, Single-Stranded, Filaggrin Proteins, Genotyping Techniques, Humans, Intermediate Filament Proteins, Microspheres, Multiplex Polymerase Chain Reaction, Nucleic Acid Hybridization, Polymorphism, Single Nucleotide, Protein Isoforms, Sensitivity and Specificity | null |
22,705,406 | 2013-04-25 | 2012-11-12 | 1873-4235 | Biosensors & bioelectronics | Label-free, needle-type biosensor for continuous glucose monitoring based on competitive binding. | Paek Sung-Ho, Cho Il-Hoon, Kim Dong-Hyung, Jeon Jin-Woo, Lim Guei-Sam, Paek Se-Hwan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Blood Glucose | IM | 22705406, S0956-5663(12)00350-8, 10.1016/j.bios.2012.05.038 | With the goal of developing a method for the continuous monitoring of blood glucose, an implantable sensor was developed by placing an optical fiber probe within the internal hollow space of a syringe needle. A glucose binder, concanavalin A (Con A), was immobilized on the probe tip and a protein (e.g., bovine serum albumin) chemically coupled with a sugar ligand (e.g., mannose) was loaded as a solution inside of the needle, which were then closed using a semi-permeable membrane. Upon immersion in the glucose sample, small molecules were able to freely pass through the membrane and compete with the ligand conjugate for Con A binding. This changed the molecular layer thickness on the probe surfaces depending on the glucose concentration, which shifted the wavelength of the guided light along the fiber. Such interference in the wavelength pattern was measured using a commercial sensor system, Octet, without employing a label. Using this analytical approach, two major steps controlling the performance of glucose detection were overcome: permeation of glucose (optimum with 50 nm-porous polycarbonate membrane under the experimental conditioned used) and molecular diffusion of the ligand conjugate within the sensor compartment (19 gauge-needle, offering minimal demensions for the probe). Under optimal conditions, the sensor was able to monitor glucose fluctuations, even in serum medium, with a response time of less than 15 min in a range 10-500 mg/dL. This, however, could be further shortened down to about 5 min in principle by miniaturizing the sensor dimensions. | Biosensing Techniques, Blood Glucose, Equipment Design, Equipment Failure Analysis, Humans, Monitoring, Ambulatory, Needles, Prostheses and Implants, Refractometry, Reproducibility of Results, Sensitivity and Specificity, Staining and Labeling | null |
22,705,410 | 2013-02-28 | 2013-11-21 | 1878-5867 | Steroids | Counteraction of early circulatory derangement by administration of low dose steroid treatment at the onset of established endotoxemic shock is not directly mediated by TNF-α and IL-6. | Söderberg Ewa, Lipcsey Miklós, Sjölin Jan, Larsson Anders, Eriksson Mats B | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Anti-Inflammatory Agents, Endotoxins, Interleukin-6, Placebos, Tumor Necrosis Factor-alpha, Hydrocortisone | IM | 22705410, S0039-128X(12)00184-5, 10.1016/j.steroids.2012.06.001 | Once a septic condition is progressing, administration of steroids in the pro-inflammatory phase of septic shock ought to yield maximal effect on the subsequent, devastating inflammatory response. Recently, a retrospective study showed that early initiation of corticosteroid therapy improved survival in septic shock. We aimed to prospectively evaluate effects of early administrated hydrocortisone therapy on physiologic variables in a porcine model of septic shock. | Animals, Anti-Inflammatory Agents, Arterial Pressure, Body Temperature, Drug Administration Schedule, Endotoxins, Heart Rate, Hydrocortisone, Inflammation, Injections, Intravenous, Interleukin-6, Male, Placebos, Random Allocation, Secondary Prevention, Shock, Septic, Swine, Tumor Necrosis Factor-alpha | null |
22,705,403 | 2012-12-10 | 2019-12-10 | 1873-4235 | Biosensors & bioelectronics | Scano-magneto immunoassay based on carbon nanotubes/gold nanoparticles nanocomposite for Salmonella enterica serovar Typhimurium detection. | Amaro Moegiratul, Oaew Sukunya, Surareungchai Werasak | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | Membranes, Artificial, Nanotubes, Carbon, Gold, Collodion | IM | 22705403, S0956-5663(12)00311-9, 10.1016/j.bios.2012.05.018 | To improve sensitivity of S. enterica serovar Typhimurium detection, multiwalled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) were combined and used as a label to amplify signal in a scanometric based assay. In this study, the MWCNTs/AuNPs nanocomposite was fabricated by directly assemble of Au(3+) to MWCNTs and allowed growing of AuNPs along the MWCNTs surface. This MWCNTs/AuNPs nanocomposite was then attached to anti-S. typhimurium antibody (MWCNTs/AuNPs/Ab(1)) and used as a detecting molecule. Upon binding to Salmonella, they were pre-concentrated by magenetic beads/antibody (MBs/Ab(2)) forming a sandwich immuno-complex which is later spotted on a nitrocellulose membrane coated slide. Silver reduction was applied to amplify signal. The detection limit of 42CFU/ml was achieved when 2% BSA was used as a blocking agent. Given different types of real samples testing, chicken broth was found to give lowest detection limit, followed by orange juice low fat and whole milk. Selectivity testing was performed by using Escherichia coli as interference and found slightly cross-reactivity which could be due to specificity of the Ab used. By virtue of using a slide for multi-samples spotting and a flatbed scanner for signal-read out acquisition, this scano-magneto immunoassay could enable low-cost detection as well as high throughput screening. | Animals, Biosensing Techniques, Collodion, Food Analysis, Food Microbiology, Gold, Humans, Limit of Detection, Membranes, Artificial, Nanocomposites, Nanoparticles, Nanotubes, Carbon, Oxidation-Reduction, Salmonella Infections, Salmonella typhimurium | null |
22,705,402 | 2012-12-10 | 2012-08-03 | 1873-4235 | Biosensors & bioelectronics | Real time monitoring of the impedance characteristics of Staphylococcal bacterial biofilm cultures with a modified CDC reactor system. | Paredes J, Becerro S, Arizti F, Aguinaga A, Del Pozo J L, Arana S | eng | null | Journal Article | null | IM | 22705402, S0956-5663(12)00339-9, 10.1016/j.bios.2012.05.027 | Detection of device-associated infectious processes is still an important clinical challenge. Bacteria grow adhered to the device surfaces creating biofilms that are resistant to antimicrobial agents, increasing mortality and morbidity. Thus there is need of a surgical procedure to remove the indwelling infected device. The elevated cost of these procedures, besides patients discomfort and increased risks, highlights the need to develop more efficient, accurate and rapid detection methods. Biosensors integrated with implantable devices will provide an effective diagnostic tool. In vivo, rapid and sensitive detection of bacteria attached to the device surfaces will allow efficient treatments. Impedance spectroscopy technique would be an adequate tool to detect the adherence and the growth of the microorganism by monitoring the impedance characteristics. In this work a label-free interdigitated microelectrode (IDAM) biosensor has been developed to be integrated with implantable devices. Impedance characterization of Staphylococcus epidermidis biofilms has been performed achieving electrical monitoring of the bacterial growths in a few hours from the onset of the infection. This pathogen represents the most common microorganism related to intravascular catheters associated infections. The experimental setup presented in this work, a modified CDC biofilm reactor, simulates the natural environment conditions for bacterial biofilm development. The results prove that the low range of frequency is the most suitable setting for monitoring biofilm development. Our findings prove the effectiveness of this technique which shows variations of 59% in the equivalent serial capacitance component of the impedance. | Bacterial Adhesion, Biofilms, Bioreactors, Biosensing Techniques, Catheters, Dielectric Spectroscopy, Electric Impedance, Equipment Design, Humans, Microelectrodes, Staphylococcal Infections, Staphylococcus epidermidis | null |
22,705,405 | 2012-12-10 | 2019-12-10 | 1873-4235 | Biosensors & bioelectronics | Electrospun phospholipid polymer substrate for enhanced performance in immunoassay system. | Chantasirichot Surasak, Ishihara Kazuhiko | eng | null | Evaluation Study, Journal Article | Antibodies, Immobilized, Immunoglobulin G, Methacrylates, Phosphorylcholine | IM | 22705405, S0956-5663(12)00341-7, 10.1016/j.bios.2012.05.029 | A functional polymer bearing both phosphorylcholine and active ester groups, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-N-succinimidyloxycarbonyl di(ethylene glycol) methacrylate (PENHS)] (PMBS), provided a highly biomolecule-friendly platform for immunoassays. The nonbiofouling property of the PMBS remarkably reduces the background noise from nonspecific adsorption of proteins in the analyte in an enzyme-linked immunosorbent assay (ELISA), which improves the specificity and signal-to-noise ratio. Electrospinning deposition, a versatile and cost-effective technique, was employed to fabricate nanofibrous PMBS. This nanostructure increased the surface area of the polymer, allowing more antibodies to bind to the polymer interface and enhancing the sensitivity of the biosensing system. The electrospun PMBS fibers were stable and retained their unique morphologies after contact with an aqueous solution for 4.0 h. The ability of PMBS to reduce background noise without blocking by protein-based reagents was verified by comparison with an immunoassay conducted on a polystyrene substrate. The ELISA of human immunoglobulin-G with the electrospun PMBS substrate showed a good sigmoidal relationship with a linear detection range from 1.0 to 100 ng/mL. The detection time was 25% shorter than the conventional assay as the blocking step was omitted. The immobilized primary antibodies exhibited high stability on the electrospun PMBS; 60% and 25% of the residual bioactivity remained after storage in dry conditions for 2 and 4 weeks, respectively. Thus, for further development of biosensors, nanostructured PMBS can improve lifetime of immobilized biomolecules, and also contribute to an enhanced reliability and signal-to-noise ratio of immunoassay. | Antibodies, Immobilized, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G, Methacrylates, Nanofibers, Phosphorylcholine, Protein Stability, Signal-To-Noise Ratio | null |
22,705,412 | 2013-04-29 | 2018-12-01 | 1873-1570 | Resuscitation | Does the bispectral index (BIS) during haemorrhagic shock and resuscitation reflect a change in cerebral perfusion or simply reflect the anaesthetic depth? | Kurita Tadayoshi, Sato Shigehito | eng | null | Letter, Comment | null | IM | 22705412, S0300-9572(12)00302-4, 10.1016/j.resuscitation.2011.08.035 | null | Animals, Brain Ischemia, Electroencephalography, Shock, Hemorrhagic | null |
22,705,411 | 2013-07-08 | 2022-03-30 | 1873-1570 | Resuscitation | Emergent pediatric thoracotomy following traumatic arrest. | Easter Joshua S, Vinton Deborah T, Haukoos Jason S | eng | null | Journal Article, Research Support, U.S. Gov't, P.H.S. | null | IM | 22705411, S0300-9572(12)00300-0, 10.1016/j.resuscitation.2012.05.024 | Emergent thoracotomy is a potentially life-saving procedure following traumatic cardiac arrest. The procedure has been studied extensively in adults, but its role in pediatric traumatic cardiac arrest remains unclear. We aimed to determine the prevalence of survival following emergent resuscitative thoracotomy in children. | Adolescent, Child, Child, Preschool, Cohort Studies, Emergency Treatment, Female, Heart Arrest, Humans, Male, Resuscitation, Retrospective Studies, Thoracic Injuries, Thoracotomy | null |
22,705,409 | 2012-12-10 | 2019-12-10 | 1873-4235 | Biosensors & bioelectronics | A glucose biosensor based on TiO2-Graphene composite. | Jang Hee Dong, Kim Sun Kyung, Chang Hankwon, Roh Ki-Min, Choi Jeong-Woo, Huang Jiaxing | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | titanium dioxide, Graphite, Titanium, Glucose Oxidase, Glucose | IM | 22705409, S0956-5663(12)00345-4, 10.1016/j.bios.2012.05.033 | A novel glucose biosensor was developed based on the adsorption of glucose oxidase at a TiO(2)-Graphene (GR) nanocomposite electrode. A TiO(2)-GR composite was synthesized from a colloidal mixture of TiO(2) nanoparticles and graphene oxide (GO) nanosheets by an aerosol assisted self-assembly (AASA). The particle morphology of all TiO(2)-GR composites was spherical in shape. It was observed that micron-sized TiO(2) particles were encapsulated by GR nanosheets and that the degree of encapsulation was proportional to the ratio of GO/TiO(2). The amperometric response of the glucose biosensor fabricated by the TiO(2)-GR composite was linear against a concentration of glucose ranging from 0 to 8mM at -0.6 V. The highest sensitivity was noted at about 6.2 μA/mMcm(2). The as prepared glucose biosensor based on the TiO(2)-GR composite showed higher catalytic performance for glucose redox than a pure TiO(2) and GR biosensor. | Biosensing Techniques, Electrochemical Techniques, Glucose, Glucose Oxidase, Graphite, Nanocomposites, Sensitivity and Specificity, Titanium | null |
22,705,404 | 2012-12-10 | 2019-12-10 | 1873-4235 | Biosensors & bioelectronics | Ambient temperature detection of PCR amplicons with a novel sequence-specific nucleic acid lateral flow biosensor. | Ang Geik Yong, Yu Choo Yee, Yean Chan Yean | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | DNA, Bacterial, Reagent Strips, Gold, Cholera Toxin | IM | 22705404, S0956-5663(12)00312-0, 10.1016/j.bios.2012.05.019 | In the field of diagnostics, molecular amplification targeting unique genetic signature sequences has been widely used for rapid identification of infectious agents, which significantly aids physicians in determining the choice of treatment as well as providing important epidemiological data for surveillance and disease control assessment. We report the development of a rapid nucleic acid lateral flow biosensor (NALFB) in a dry-reagent strip format for the sequence-specific detection of single-stranded polymerase chain reaction (PCR) amplicons at ambient temperature (22-25°C). The NALFB was developed in combination with a linear-after-the-exponential PCR assay and the applicability of this biosensor was demonstrated through detection of the cholera toxin gene from diarrheal-causing toxigenic Vibrio cholerae. Amplification using the advanced asymmetric PCR boosts the production of fluorescein-labeled single-stranded amplicons, allowing capture probes immobilized on the NALFB to hybridize specifically with complementary targets in situ on the strip. Subsequent visual formation of red lines is achieved through the binding of conjugated gold nanoparticles to the fluorescein label of the captured amplicons. The visual detection limit observed with synthetic target DNA was 0.3 ng and 1 pg with pure genomic DNA. Evaluation of the NALFB with 164 strains of V. cholerae and non-V. cholerae bacteria recorded 100% for both sensitivity and specificity. The whole procedure of the low-cost NALFB, which is performed at ambient temperature, eliminates the need for preheated buffers or additional equipment, greatly simplifying the protocol for sequence-specific PCR amplicon analysis. | Base Sequence, Biosensing Techniques, Cholera, Cholera Toxin, DNA, Bacterial, Gold, Humans, Limit of Detection, Molecular Sequence Data, Nanoparticles, Polymerase Chain Reaction, Reagent Strips, Sensitivity and Specificity, Temperature, Vibrio cholerae | null |
22,705,414 | 2012-11-19 | 2013-11-21 | 1879-1700 | Journal of environmental radioactivity | Chemical studies on polyaniline titanotungstate and its uses to reduction cesium from solutions and polluted milk. | El-Naggar I M, Zakaria E S, Ali I M, Khalil M, El-Shahat M F | eng | null | Journal Article | Aniline Compounds, Metals, Oxides, Radioactive Pollutants, Tungsten Compounds, Water Pollutants, Chemical, polyaniline, tungsten titanate, Cesium | IM | 22705414, S0265-931X(12)00126-9, 10.1016/j.jenvrad.2012.05.012 | Polyaniline titanotungstate (PATiW) was synthesized by the sol-gel method. Adsorption isotherm studies of Cs(+) from aqueous solution are described. Elemental Composition, chemical solubility, ion-exchange capacity (IEC) and pH-titration curve are studied. Distribution coefficients (K(d)) for 10 metal ions were determined. It was found that the polyaniline titanotungstate is highly selective to Cs(+) and the selectivity order is Cs(+)>>>Zr(4+)>Mo(6+)>V(5+)>As(5+)>Cr(3+)>Co(2+)>Cu(2+)>Zn(2+)>Cd(2+). The adsorbent capacity was determined using the Freundlich and Langmuir adsorption isotherm models. The Cs(+) adsorption isotherm data fit best to the Freundlich isotherm model. The maximum Cs(+) uptake of polyaniline titanotungstate was found to be 217 mg g(-1). Column tests were performed to determine the breakthrough curves with varying bed depths and flow rates in different solutions. The results showed that the half breakthrough time increases proportionally with increasing bed depths. Kinetic studies for removal of cesium from milk were also investigated using a scintillation detector head (NaI). | Adsorption, Aniline Compounds, Animals, Cattle, Cesium, Half-Life, Ion Exchange, Kinetics, Metals, Milk, Oxides, Radioactive Pollutants, Tungsten Compounds, Water Pollutants, Chemical | null |
22,705,407 | 2012-12-10 | 2021-12-03 | 1873-4235 | Biosensors & bioelectronics | An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum. | Shiddiky Muhammad J A, Kithva Prakash H, Kozak Darby, Trau Matt | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | Acrylamides, Acrylic Resins, Antibodies, Immobilized, Antigens, Neoplasm, Blood Proteins, Cadmium Compounds, GPI-Linked Proteins, MSLN protein, human, Polymers, Selenium Compounds, Single-Chain Antibodies, poly-N-isopropylacrylamide, cadmium selenide, Mesothelin | IM | 22705407, S0956-5663(12)00294-1, 10.1016/j.bios.2012.05.014 | An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) 'brush' layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying 'bionanoconjugate' comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards MSLN, is used to 'physically' amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD=4.6%, n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics. | Acrylamides, Acrylic Resins, Adsorption, Antibodies, Immobilized, Antigens, Neoplasm, Biosensing Techniques, Blood Proteins, Cadmium Compounds, Electrochemical Techniques, Female, GPI-Linked Proteins, Humans, Immunoassay, Mesothelin, Ovarian Neoplasms, Polymerization, Polymers, Quantum Dots, Selenium Compounds, Sensitivity and Specificity, Single-Chain Antibodies | null |
22,705,408 | 2012-12-10 | 2019-12-10 | 1873-4235 | Biosensors & bioelectronics | SU-8-carbon composite as conductive photoresist for biochip applications. | Benlarbi Mouhssine, Blum Loïc J, Marquette Christophe A | eng | null | Evaluation Study, Journal Article | DNA Probes, Polymers, Carbon, DNA | IM | 22705408, S0956-5663(12)00338-7, 10.1016/j.bios.2012.05.026 | A composite photoresist has been developed for the direct photopatterning of electrodes useful as biochip substrates. The material is composed of SU-8 polymer added with graphite carbon filler which enables patterning of conductive thin films (22 μm) on both glass substrate and transparency flexible film with a standard UV photolithography protocol. The resolution obtained using the conductive composite compared well with the bare resist, with lateral resolutions of 5 and 10 μm for bare and conductive resists, respectively. The obtained electrodes, after an electrochemical pre-treatment, exhibited very good electrochemical behaviors, opening the path to various electrochemical detections and grafting possibilities. In order to demonstrate the potentialities of the developed material in the biosensors and biochips field, DNA probes were electrografted, using diazonium chemistry, directly at the composite photoresist surface. Target oligonucleotide interactions were detected using chemiluminescent labeling and a satisfactory detection limit of 0.25 nM target sequence was demonstrated with a detection ranging over three orders of magnitude. | Carbon, DNA, DNA Probes, Electric Conductivity, Electrochemical Techniques, Electrodes, Equipment Design, Limit of Detection, Luminescent Measurements, Nanocomposites, Oligonucleotide Array Sequence Analysis, Photochemistry, Polymers | null |
22,705,415 | 2012-11-19 | 2013-11-21 | 1879-1700 | Journal of environmental radioactivity | Determination of dose rate from Chernobyl-derived radiocaesium in Estonian soil. | Lust M, Realo E | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cesium Radioisotopes, Radioactive Fallout, Soil Pollutants, Radioactive, Cesium | IM | 22705415, S0265-931X(12)00135-X, 10.1016/j.jenvrad.2012.05.021 | The deposition and vertical depth distribution of 134Cs and 137Cs in the natural undisturbed soil profiles down to 20-25 cm were studied at locations in the North Eastern Estonia, which were most strongly affected by the Chernobyl fallout in 1986. The total depositions were estimated based on summing the sampled and measured activities of 134Cs and 137Cs in all sections of soil profiles. The Chernobyl 137Cs deposition values varied considerably from site to site and the range was from 3.9 kBq m(-2) to 50.2 kBq m(-2), with the average of 22.8 kBq m(-2) for the region (reference data May 1, 1986). The ratio of total activities, A(134Cs)/A(137Cs), varied in the range from 0.47 to 0.55. Using a compartment model and the observed data on the 134Cs and 137Cs activity concentrations (Bq m(-2)) in four compartments, 0-1 cm, 1-5 cm, 5-15 cm and 15-30 cm, of soil collected in 1991-2003, the approximate residence half-times of radiocaesium in soil were determined. The latter increased from 3.7 y in the top-most compartment to 8.6 y and 36.4 y in the deeper compartments, respectively. The time dependence of the external gamma-dose rate at the height of 1 m above a flat ground area arising from the deposited and migrating radiocaesium was calculated using the modeled data on the 134Cs and 137Cs activity in soil compartments. Considering the total depositions of radiocaesium in soil from the Chernobyl 1986 accident, the 50 year effective doses caused by external gamma exposure varied in the range from 0.13 mSv to 1.74 mSv, with the mean of 0.79 mSv in the region. | Cesium, Cesium Radioisotopes, Chernobyl Nuclear Accident, Environmental Exposure, Estonia, Humans, Models, Theoretical, Radiation Dosage, Radiation Monitoring, Radioactive Fallout, Seasons, Soil Pollutants, Radioactive | null |
22,705,413 | 2013-07-10 | 2024-05-12 | 1873-5118 | Progress in neurobiology | CACNA1C (Cav1.2) in the pathophysiology of psychiatric disease. | Bhat Shambhu, Dao David T, Terrillion Chantelle E, Arad Michal, Smith Robert J, Soldatov Nikolai M, Gould Todd D | eng | L30 MH091729 (NIMH NIH HHS, United States); R21 MH093967 (NIMH NIH HHS, United States); MH093967 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | CACNA1C protein, human, Calcium Channels, L-Type | IM | 22705413, S0301-0082(12)00099-8, 10.1016/j.pneurobio.2012.06.001, PMC3459072, NIHMS395345, 11041323, 22614287, 22012475, 12742871, 10813800, 15465978, 10341236, 19388002, 21519340, 18195452, 8210181, 11598293, 15299022, 12140781, 21248242, 21897434, 20169575, 21940447, 19781653, 20595427, 3037387, 19360908, 20194825, 21926972, 16566899, 19065143, 9832975, 19621016, 17248194, 11007898, 16458265, 18395805, 19665524, 11281512, 16988657, 7612157, 15152039, 18348202, 1386279, 11572963, 4531429, 12163037, 12617940, 9210749, 2173707, 18441296, 2664242, 16252380, 16154277, 20190743, 17898207, 17681086, 11252650, 20638048, 12724155, 2735813, 21926974, 2160087, 20636642, 16099045, 17916557, 14677076, 18317468, 3622617, 7623135, 19215628, 8062423, 15329393, 20016080, 2174714, 8629888, 20616314, 17823125, 19900610, 14665691, 9892254, 20351721, 20098439, 18427557, 21159975, 16275756, 21037240, 12183620, 7959794, 9435762, 19150704, 14512831, 19952088, 8392192, 22614292, 19029287, 22665259, 20679588, 10764893, 21767209, 22464935, 19648911, 21685391, 18516516, 20819988, 15146240, 11861774, 12716953, 16369047, 10973973, 8980227, 21383011, 15090038, 12077219, 20351715, 17554300, 15454078, 22665262, 19840220, 1653763, 14506311, 20573883, 7907431, 15140941, 9790159, 14601037, 11288158, 21746798, 16355250, 19559597, 18250309, 21078228, 22499782, 21857009, 20732952, 21450352, 15261117, 16251435, 15863612, 21430174, 8200343, 12668349, 18698231, 17912248, 8888378, 12388619, 19564422, 12832067, 20886543, 10748141, 8032872, 20929969, 19047462, 1316612, 18174367, 17071743, 2725849, 21292451, 20723887, 22085483, 11751866, 17689567, 15285847, 10335846, 11356668, 3224810, 20843874, 11346797, 19594501, 20125088, 18815371, 2474130, 17001293, 9515967, 21676128, 19065144, 18045623, 18711365, 21878566 | One of the most consistent genetic findings to have emerged from bipolar disorder genome wide association studies (GWAS) is with CACNA1C, a gene that codes for the α(1C) subunit of the Ca(v)1.2 voltage-dependent L-type calcium channel (LTCC). Genetic variation in CACNA1C have also been associated with depression, schizophrenia, autism spectrum disorders, as well as changes in brain function and structure in control subjects who have no diagnosable psychiatric illness. These data are consistent with a continuum of shared neurobiological vulnerability between diverse-Diagnostic and Statistical Manual (DSM) defined-neuropsychiatric diseases. While involved in numerous cellular functions, Ca(v)1.2 is most frequently implicated in coupling of cell membrane depolarization to transient increase of the membrane permeability for calcium, leading to activation and, potentially, changes in intracellular signaling pathway activity, gene transcription, and synaptic plasticity. Ca(v)1.2 is involved in the proper function of numerous neurological circuits including those involving the hippocampus, amygdala, and mesolimbic reward system, which are strongly implicated in psychiatric disease pathophysiology. A number of behavioral effects of LTCC inhibitors have been described including antidepressant-like behavioral actions in rodent models. Clinical studies suggest possible treatment effects in a subset of patients with mood disorders. We review the genetic structure and variation of CACNA1C, discussing relevant human genetic and clinical findings, as well as the biological actions of Ca(v)1.2 that are most relevant to psychiatric illness. | Calcium Channels, L-Type, Genetic Variation, Humans, Mental Disorders | null |
22,705,416 | 2012-11-19 | 2017-11-16 | 1879-1700 | Journal of environmental radioactivity | Using 137Cs to study spatial patterns of soil erosion and soil organic carbon (SOC) in an agricultural catchment of the typical black soil region, Northeast China. | Fang Haiyan, Li Qiuyan, Sun Liying, Cai Qiangguo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cesium Radioisotopes, Soil, Soil Pollutants, Radioactive, Cesium, Carbon | IM | 22705416, S0265-931X(12)00132-4, 10.1016/j.jenvrad.2012.05.018 | Understanding the spatial pattern of soil organic carbon (SOC) is of great importance because of global environmental concerns. Soil erosion and its subsequent redistribution contribute significantly to the redistribution of SOC in agricultural ecosystems. This study investigated the relationships between (137)Cs and SOC over an agricultural landscape, and SOC redistribution was conducted for an agricultural catchment of the black soil region in Northeast China. The spatial patterns of (137)Cs and SOC were greatly affected by the established shelterbelts and the developed ephemeral gullies. (137)Cs were significantly correlated with SOC when (137)Cs were >2000 Bq m(-2), while no relation was observed between them when (137)Cs were <2000 Bq m(-2). Factors other than soil erosion such as vegetative productivity, mineralization of SOC, landscape position and management induced their spatial difference of (137)Cs and SOC. Using (137)Cs technique to directly study SOC dynamics must be cautious in the black soils. The net SOC loss rate across the entire catchment during 1954-2010 was 92.8 kg ha(-1) yr(-1), with around 42% of the eroded SOC being redeposited within the catchment. Such information can help guide shelterbelt establishment or other land management to reduce SOC loss in the agricultural ecosystems. | Agriculture, Carbon, Cesium, Cesium Radioisotopes, China, Radiation Monitoring, Soil, Soil Pollutants, Radioactive | null |
22,705,418 | 2012-11-29 | 2013-11-21 | 1872-7492 | Virus research | Mechanical properties of viruses analyzed by atomic force microscopy: a virological perspective. | Mateu Mauricio G | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | null | IM | 22705418, S0168-1702(12)00205-5, 10.1016/j.virusres.2012.06.008 | The advent of nanoscience and nanotechnology and the development of atomic force microscopy and other single-molecule techniques are leading to a renewed look at viruses from the point of view of the physical sciences. As any other solid-state object, virus particles are endowed with mechanical properties such as elasticity or brittleness. Emerging studies on virus mechanics may facilitate the engineering of the physical properties of viruses to improve their potential application in nanotechnology, and may be also relevant to understand virus biology. Viruses are subject to internal and external forces, and as evolving entities they may have selectively adapted their mechanical behavior to resist, or even use, those forces. This article adopts the perspective of structural and molecular virology to review the results obtained to date, using the atomic force microscope, on the mechanical properties of virus particles, their molecular determinants, and possible biological implications. | Animals, Biomechanical Phenomena, Elasticity, Humans, Microscopy, Atomic Force, Virology, Viruses | null |
22,705,419 | 2012-09-18 | 2019-12-10 | 0027-5107 | Mutation research | Use of β-galactosidase (lacZ) gene α-complementation as a novel approach for assessment of titanium oxide nanoparticles induced mutagenesis. | Ahmad Javed, Dwivedi Sourabh, Alarifi Saud, Al-Khedhairy Abdulaziz A, Musarrat Javed | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't | titanium dioxide, Titanium, beta-Galactosidase | IM | 22705419, S1383-5718(12)00222-7, 10.1016/j.mrgentox.2012.06.002 | The mutagenic potential of titanium dioxide nanoparticles (TiO(2)-NPs) of an average size 30.6nm was investigated using β-galactosidase (lacZ) gene complementation in plasmid pUC19/lacZ(-)Escherichia coli DH5α system. Plasmid pUC19 was treated with varying concentrations of TiO(2)-NPs and allowed to transfect the CaCl(2)-induced competent DH5α cells. The data revealed loss in transformation efficiency of TiO(2)-NPs treated plasmids as compared to untreated plasmid DNA in DH5α host cells. Induction of multiple mutations in α-fragment of lacZ gene caused synthesis of non-functional β-galactosidase enzyme, which resulted in a significant number of white (mutant) colonies of transformed E. coli cells. Screening of mutant transformants based on blue:white colony assay and DNA sequence analysis of lacZ gene fragment clearly demonstrated TiO(2)-NPs induced mutagenesis. Multiple alignment of selectable marker lacZ gene sequences from randomly selected mutants and control cells provided a gene specific map of TiO(2)-NPs induced mutations. Mutational analysis suggested that all nucleotide changes were point mutations, predominantly transversions (TVs) and transitions (TSs). A total of 32 TVs and 6 TSs mutations were mapped within 296 nucleotides (nt) long partial sequence of lacZ gene. The region between 102 and 147nt within lacZ gene sequence was found to be most susceptible to mutations with nine detectable point mutations (8 TVs and 1 TSs). Guanine base was determined to be more prone to TiO(2)-NPs induced mutations. This study suggested the pUC19/E. coli DH5αlacZ gene α-complementation system, as a novel genetic approach for determining the mutagenic potential, and specificity of manufactured NPs and nanomaterials. | Cell Survival, Escherichia coli, Genetic Complementation Test, Mutagenicity Tests, Nanoparticles, Titanium, Transfection, Transformation, Bacterial, beta-Galactosidase | null |
22,705,421 | 2012-08-30 | 2018-12-01 | 0964-7058 | Asia Pacific journal of clinical nutrition | Sasang types may differ in eating rate, meal size, and regular appetite: a systematic literature review. | Pham Duong Duc, Lee Jae Chul, Lee Myeong Soo, Kim Jong Yeol | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review, Systematic Review | null | IM | 22705421 | Eating behaviours may be implicated in the increasing prevalence of obesity and metabolic disorders. The Sasang typology, a unique form of traditional Korean medicine, classifies individuals into four constitutional types that differ in a distinctive complex of external manifestations and innate natures, including eating behaviours. Our aim is to portray a picture of the distinguishing characteristics of eating behaviours across Sasang types and to provide suggestions for future studies. Six Korean and one English database were searched to acquire relevant articles. Ten peer-reviewed relevant research articles were found. The extracted data were categorised into the domains of i) food preferences; ii) eating rate; iii) eating initiation and termination; iv) meal size; v) regularity of eating; vi) regular appetite; vii) eating disorders; and viii) psychological factors. Eating rate and meal size were the issues of most concern that more frequently were different among Sasang types. The TaeEum type seemed to have obesity-linked eating behaviours, including a rapid eating rate, large meal sizes, and a strong appetite, whereas those attitudes seemed to be in contrast with those of the SoEum type. The SoYang type shared similarities with both the TaeEum and SoEum types. Future studies should be conducted with more reliable, objective, and quantitative assessment tools such as the Three Eating Factor Questionnaire or the Dutch Eating Behaviour Questionnaire. | Adolescent, Adult, Aged, Appetite, Child, Democratic People's Republic of Korea, Diet, Eating, Feeding Behavior, Food Preferences, Humans, Medicine, Korean Traditional, Metabolic Diseases, Overweight, Republic of Korea, Young Adult | null |
22,705,420 | 2012-08-30 | 2021-12-03 | 0964-7058 | Asia Pacific journal of clinical nutrition | An original discovery: selenium deficiency and Keshan disease (an endemic heart disease). | Chen Junshi | eng | null | Journal Article, Review | Selenium, Sodium Selenite | IM | 22705420 | This is a review article telling a 50-years old story about the studies on selenium deficiency and Keshan disease in China, an endemic heart disease with high case-fatality, as an example of translational research. Extensive cross-sectional epidemiological studies showed that low selenium concentrations in cereal grains and low selenium status of local residents were associated with the occurrence of Keshan disease. Several large population based intervention trials using oral administration of sodium selenite tablets showed significant reduction of Keshan disease incidence. Based on the above evidence, it was concluded that selenium deficiency is the major cause of Keshan disease, although other etiological factors could not be ruled out. The implications of the findings include: provided critical scientific evidence for selenium being an essential trace element for humans; as scientific basis for identifying minimum requirement and RDA/RNI for selenium; and as solid reference for the formulation of effective preventive measures for Keshan disease in China. | Adult, Cardiomyopathies, Child, China, Diet, Dietary Supplements, Edible Grain, Endemic Diseases, Enterovirus Infections, Female, Humans, Male, Nutritional Requirements, Selenium, Sodium Selenite, Translational Research, Biomedical | null |
22,705,417 | 2013-01-07 | 2021-10-21 | 1873-2534 | Veterinary immunology and immunopathology | Virus-like particles: the new frontier of vaccines for animal viral infections. | Crisci Elisa, Bárcena Juan, Montoya María | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Vaccines, Virus-Like Particle | IM | 22705417, S0165-2427(12)00181-X, 10.1016/j.vetimm.2012.04.026, PMC7112581, 19707432, 17542753, 9062924, 10710215, 8113722, 21784230, 21492483, 17434554, 18983932, 17630337, 18588935, 1313899, 18083549, 18378364, 20923881, 10393897, 10196348, 16485473, 8752296, 19157482, 12034099, 15063122, 15819426, 16904154, 10051649, 10051569, 9015109, 19198568, 18251693, 17259949, 22491457, 8856024, 8811015, 909009, 11356839, 16690856, 18927950, 8847514, 16997708, 7483793, 21721420, 11044077, 9143688, 12438590, 21895749, 11974620, 19889768, 20047040, 10915608, 21858228, 15877604, 20872839, 12400999, 17989184, 9167911, 17126499, 16730104, 10640566, 2973187, 1696684, 8084017, 12163261, 16997719, 10596801, 20961433, 22306796, 15613299, 18842943, 18706958, 20352110, 16997714, 21762752, 21314901, 19327809, 21554858, 11530699, 16336922, 21784144, 8524802, 18022293, 19476953, 14505922, 21987741, 21864590, 17546687, 21816199, 13678860, 20923267, 19773133, 8957677, 10067709, 1371541, 18511159, 17931113, 16632527, 21651936, 21813755, 19374929, 19348559, 9207121, 19733890, 1781157, 20433802, 11884458, 19344926, 16784246, 9157156, 15542679, 10753814, 20181713, 16122848, 16375716, 10741972, 18953718, 18438104, 1369994, 7541982, 21784228, 21449023, 18304705, 19768411, 19167843, 11395200, 18237827, 20199612, 10502819, 21539868, 16797803, 14499247, 12804866, 15207617, 1658362, 10982326, 15322175, 21806400, 17277142, 12922123, 2446137, 22265860, 21216540, 1311487, 17227681, 8957674, 20948547, 11115693, 19184804, 15812483, 15560977, 21316403, 2157868, 8411366, 19379787, 20378136, 9689105, 12748380, 21339837, 20455698, 9491505, 17005697, 19100779, 8248784, 12741758, 7745754 | Vaccination continues to be the main approach to protect animals from infectious diseases. Until recently, all licensed vaccines were developed using conventional technologies. Subunit vaccines are, however, gaining attention from researchers in the field of veterinary vaccinology, and among these, virus-like particles (VLPs) represent one of the most appealing approaches. VLPs are robust protein cages in the nanometer range that mimic the overall structure of the native virions but lack the viral genome. They are often antigenically indistinguishable from the virus from which they were derived and present important advantages in terms of safety. VLPs can stimulate strong humoral and cellular immune responses and have been shown to exhibit self-adjuvanting abilities. In addition to their suitability as a vaccine for the homologous virus from which they are derived, VLPs can also be used as vectors for the multimeric presentation of foreign antigens. VLPs have therefore shown dramatic effectiveness as candidate vaccines. Here, we review the current status of VLPs as a vaccine technology in the veterinary field, and discuss the potential advantages and challenges of this technology. | Animals, Chickens, Poultry Diseases, Swine, Swine Diseases, Vaccines, Virus-Like Particle, Virus Diseases | null |
22,705,423 | 2012-08-30 | 2019-12-10 | 0964-7058 | Asia Pacific journal of clinical nutrition | Height prediction from anthropometric length parameters in Thai people. | Chittawatanarat Kaweesak, Pruenglampoo Sakda, Trakulhoon Vibul, Ungpinitpong Winai, Patumanond Jayanton | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study | null | IM | 22705423 | Height is an important clinical parameter. However, there were no specific measurements available for particular clinical situations. Although many anthropometric measurements were suggested, no formula was recommended in Thailand. The objective of this study was to develop a formula for height prediction with acceptable validity. Two thousand volunteers were included and were divided consecutively according to both age and gender. Model and validation groups were further separated independently. Linear regression was analyzed to create a predictive formula. Ten parameters were included and analyzed. Of these, demispan, sitting height and knee height were selected with a correlation coefficient of more than 0.5 and significant F test in all age groups and genders. All single parameters and the highest predictive value of double (sitting and knee height) and triple regression models (demispan, sitting and knee height) were proposed and these were modified into a simple formula. After validation of both formulas the correlation, quantitative error and relative error were comparable. The simple formula had more than 90% precision with an error of up to 10 cm in the validation group (89.7 to 99.0% in range). Of these, knee height had the least predictive error in all subgroups. The double and triple models had decreased error only in the younger group. In summary, anthropometric parameters with demispan, sitting height, knee height and combination could be applied to height prediction in the adult Thai with acceptable error. These formulas should be applied only in people who could not be directly measured. | Adolescent, Adult, Age Factors, Aged, Algorithms, Anthropometry, Body Height, Body Weights and Measures, Female, Humans, Linear Models, Male, Middle Aged, Models, Biological, Sex Characteristics, Thailand, Young Adult | null |
22,705,422 | 2012-08-30 | 2022-03-21 | 0964-7058 | Asia Pacific journal of clinical nutrition | Comparison between measured and predicted resting energy expenditure in mechanically ventilated patients with COPD. | Rao Zhi-Yong, Wu Xiao-Ting, Wang Mao-Yun, Hu Wen | eng | null | Comparative Study, Journal Article, Randomized Controlled Trial | null | IM | 22705422 | The aim of this study was to compare resting energy expenditure (REE) obtained by indirect calorimetry (IC) and Harris-Benedict (H-B) equations, and to examine whether hypocaloric nutrition support could improve protein nutritional status in mechanically ventilated patients with chronic obstructive pulmonary disease (COPD). Thirtythree COPD patients (20 males, 13 females) were recruited and REE was measured by IC. Measured REE (REEm) was compared to predictive REE by H-B equations (REEH-B) and its corrected values. Correlation between REEm and APACHE II score was also analyzed. Patients were randomly divided into hypocaloric energy group (50%-90% of REEm, En-low) and general energy group (90%-130% of REEm, En-gen) for nutrition support. The differences of albumin, prealbumin, transferrin, hemoglobin, and lymphocyte count before and after 7 days nutrition support were observed. Results show that REEH-B and REEH-B×1.2 were significantly lower than REEm (p<0.01). REEm positively correlated with APACHE II score (p<0.05 or p<0.01). After nutrition support, hemoglobin decreased significantly in En-gen group (p<0.05); lymphocyte count in both groups, and transferrin and prealbumin in the En-low group increased significantly (p<0.05 or p<0.01). Our data suggest that 1) these patients' REE were increased; 2) since IC is the best method to determine REE, in the absence of IC, H-B equations (with standard body weight) can be used to calculate REE, but the value should be adjusted by correction coefficients derived from APACHE II; 3) low energy nutrition support during mechanical ventilation in COPD patients might have better effects on improving protein nutritional status than high energy support. | APACHE, Aged, Aged, 80 and over, Algorithms, Basal Metabolism, Body Weight, Caloric Restriction, Calorimetry, Indirect, Female, Humans, Male, Malnutrition, Middle Aged, Nutritional Requirements, Nutritional Status, Pulmonary Disease, Chronic Obstructive, Respiration, Artificial, Stress, Physiological | null |
22,705,424 | 2012-08-30 | 2021-10-21 | 0964-7058 | Asia Pacific journal of clinical nutrition | Development and evaluation of a semi-quantitative food frequency questionnaire for use in urban and rural India. | Bowen Liza, Bharathi Ankalmadagu Venkatsubbareddy, Kinra Sanjay, Destavola Bianca, Ness Andy, Ebrahim Shah | eng | 084674 (Wellcome Trust, United Kingdom) | Evaluation Study, Journal Article | null | IM | 22705424 | The objective of this study was to develop and evaluate a semi-quantitative food frequency questionnaire (FFQ) for use in urban and rural India. A single FFQ was developed for use in cities and rural areas of four regions of India. To assess validity, the FFQ was administered to 530 factory workers and rural dwellers, and subsequently three 24 hour recalls were administered on different days. Nutrient and food group intake calculated from these two methods were compared using medians, kappa statistics, Spearman's correlation coefficients and Bland- Altman plots. Dietary intake was overestimated by the FFQ compared to the 24 hour recalls (mean difference in energy intake = 1743 kJ), with kappa statistics ranging from 0.07 (egg) to 0.51 (carbohydrate). The results showed acceptable validity for measuring intakes of groups, and demonstrated that it is feasible to measure dietary intake in diverse regions of India with a single FFQ. | Adolescent, Adult, Diet, Energy Intake, Female, Humans, India, Male, Middle Aged, Nutrition Surveys, Rural Health, Surveys and Questionnaires, Urban Health, Young Adult | null |
22,705,426 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | Supplementary feeding with locally-produced Ready-to-Use Food (RUF) for mildly wasted children on Nias Island, Indonesia: comparison of daily and weekly program outcomes. | Purwestri Ratna C, Scherbaum Veronika, Inayati Dyah A, Wirawan Nia N, Suryantan Julia, Bloem Maurice A, Pangaribuan Rosnani V, Stuetz Wolfgang, Hoffmann Volker, Qaim Matin, Biesalski Hans K, Bellows Anne C | eng | null | Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | null | IM | 22705426 | Ready-to-Use Foods (RUFs) in the form of fortified cereal/nut/legume-based biscuits (±500 kcal and 8-10% protein per 100 g) were tested among mildly wasted children from October 2007 to June 2008, and were labelled as RUF-Nias biscuits. This study reports on a comparison of supplementary feeding program outcomes of mildly wasted children with weight-for-height z-score (WHZ) >=-2 to <-1.5 SD aged >=6 to <60 months old given locally produced RUF-Nias biscuits within daily (in semi-urban areas) and weekly (in rural remote regions) distribution and supervision program settings. In the Church World Service project area, all eligible children were recruited continuously from monthly community-based screening programs and admitted into existing nutrition centers managed by the community on Nias Island, Indonesia. Individual discharge criterion of the programs was WHZ >=-1.5 SD. Of the index children admitted in daily programs (n=51), 80.4% reached target WHZ, which was higher than in weekly programs (72.9%; n=48) by a similar length of stay of about 6 weeks. Weight gain of the children in daily programs was higher (3.1±3.6 g/kg body weight/day) than in weekly programs (2.0±2.1 g/kg body weight/day), and they achieved significantly higher WHZ at discharge. However, the majority of caretakers preferred weekly programs due to lower time constraints. Locally produced RUF in the form of biscuits for treatment of mild wasting among children demonstrated promising results both in daily and weekly community-based intervention programs. | Bread, Caregivers, Child Development, Child, Preschool, Community Health Services, Dairy Products, Diet, Fast Foods, Feeding Methods, Female, Humans, Indonesia, Infant, Infant Food, Longitudinal Studies, Male, Patient Preference, Poverty Areas, Severity of Illness Index, Wasting Syndrome, Weight Gain | null |
22,705,425 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | Combined intensive nutrition education and micronutrient powder supplementation improved nutritional status of mildly wasted children on Nias Island, Indonesia. | Inayati Dyah A, Scherbaum Veronika, Purwestri Ratna C, Wirawan Nia N, Suryantan Julia, Hartono Susan, Bloem Maurice A, Pangaribuan Rosnani V, Biesalski Hans K, Hoffmann Volker, Bellows Anne C | eng | null | Journal Article, Randomized Controlled Trial | Hemoglobins, Iron, Dietary, Micronutrients | IM | 22705425 | To assess the impact of intensive nutrition education (INE) with or without the provision of micronutrient powder (MNP) on the nutritional status of mildly wasted children in Nias, Indonesia, two groups of mildly wasted (>=-1.5 to <-1.0 WHZ) children aged >=6 to <60 months in the Church World Service (CWS) project areas were assigned by village randomization to receive INE (n=64) or INE+MNP (n=51) in a weekly program. Another two groups of mildly wasted children who were living at a clear distance from INE and INE+MNP villages were selected to receive a monthly non-intensive nutrition education program (NNE) with or without MNP (n=50 both respectively). WHZ, weight, height, haemoglobin (Hb) level, and morbidity data were assessed at admission, during the study, and at individual discharge. Children's weight gain (g/kg body weight/day) was highest in INE+MNP group (2.2±2.1), followed by INE (1.1±0.9), NNE+MNP (0.3±0.5) and NNE (0.3±0.4) group. In both MNP intervention groups (INE+MNP, NNE+MNP), supplements significantly increased Hb value (g/L) of respective children (10.0±10.0; p<0.001 and 3.0±8.0; p<0.05 respectively). Proportion of children who reached discharge criterion was highest among the INE+MNP (70.6%; n=36), followed by INE (64.1%; n=41), NNE+MNP (26.0%; n=13), and NNE (20.0%; n=10) groups (p<0.001). Shortest length of stay until recovery was observed among children in the INE+MNP group (29.9 days), followed by INE (40.0 days), NNE+MNP (80.6 days), and NNE (86.2 days) respectively (p<0.001). Weekly intensive nutrition education supported by MNP supplementation produced the best results regarding weight gain and haemoglobin status of mildly wasted children. | Anemia, Iron-Deficiency, Child Development, Child, Preschool, Dietary Supplements, Female, Follow-Up Studies, Hemoglobins, Humans, Indonesia, Infant, Iron, Dietary, Male, Micronutrients, Nutritional Sciences, Nutritional Status, Patient Education as Topic, Poverty Areas, Severity of Illness Index, Wasting Syndrome, Weight Gain | null |
22,705,428 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | The complexity of treating wasting in ambulatory rehabilitation: Is it starvation, sarcopenia, cachexia or a combination of these conditions? | Yaxley Alison, Miller Michelle D, Fraser Robert J, Cobiac Lynne, Crotty Maria | eng | null | Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | C-Reactive Protein | IM | 22705428 | Nutritional status is often impaired in ambulatory rehabilitation patients. Wasting conditions can be classified as starvation, sarcopenia or cachexia but differences between these are not well defined, and misdiagnosis may lead to inappropriate intervention. A secondary analysis of data from 187 ambulatory rehabilitation patients aged >=60 years aimed to identify patients with one or more wasting condition, and investigate the impact on common rehabilitation outcomes. Starvation was defined by fat-free mass index and the Council on Nutrition Appetite Questionnaire score; sarcopenia by fat-free mass index and quadriceps strength; and cachexia by fat-free mass index and serum C-reactive protein. Selected rehabilitation outcomes were compared for those who were, and those who were not, identified as having one or more wasting condition. Of those identified with starvation (n=30), all were also identified as sarcopenic and 20 as cachectic; of those identified as sarcopenic (n=75), 30 had starvation and 37 were cachectic; and of those identified as cachectic (n=37), 20 had starvation and all were sarcopenic. Twenty participants were identified as having all three conditions. Those with starvation had higher level of depression (p=0.003), lower self-rated health (p=0.032), and lower levels of physical function (motor p=0.006; process p=0.004) than those with no evidence of a wasting condition. Those who had sarcopenia had lower physical function (motor p=0.012; process p=0.003) as did those with cachexia (motor p=0.025; process p=0.042). Results suggest problems in operationalising definitions in an ambulatory clinical setting. The overlap identified in this analysis suggests that up to 40% (75/187) of patients could be misidentified and prescribed inappropriate nutritional support. | Aged, Aged, 80 and over, Ambulatory Care Facilities, Attitude to Health, Body Composition, Body Mass Index, C-Reactive Protein, Cachexia, Cross-Sectional Studies, Depression, Diagnosis, Differential, Female, Humans, Male, Middle Aged, Muscle Strength, Quadriceps Muscle, Rehabilitation Centers, Sarcopenia, South Australia, Starvation, Wasting Syndrome | null |
22,705,427 | 2012-08-30 | 2013-11-21 | 0964-7058 | Asia Pacific journal of clinical nutrition | Glutamine for chemotherapy induced diarrhea: a meta-analysis. | Sun Juxian, Wang Hui, Hu Heping | eng | null | Journal Article, Meta-Analysis | Antidiarrheals, Antineoplastic Agents, Glutamine | IM | 22705427 | The clinical efficacy of glutamine in the control of chemotherapy-induced diarrhea remains controversial. We conducted a meta-analysis, including as many randomized control trails (RCTs) as possible, to clarify the effectiveness of prophylactic glutamine in patients requiring chemotherapy. | Antidiarrheals, Antineoplastic Agents, Diarrhea, Enteral Nutrition, Glutamine, Humans, Neoplasms, Parenteral Nutrition, Randomized Controlled Trials as Topic | null |
22,705,429 | 2012-08-30 | 2013-11-21 | 0964-7058 | Asia Pacific journal of clinical nutrition | Plasma phospholipid polyunsaturated fatty acids and homocysteine in Chinese type 2 diabetes patients. | Huang Tao, Asimi Sailimuhan, Lou Dajun, Li Duo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fatty Acids, Omega-3, Fatty Acids, Omega-6, Fatty Acids, Unsaturated, Phospholipids, Homocysteine, Folic Acid, Vitamin B 12 | IM | 22705429 | The main aim of the present study was to investigate the plasma phospholipids (PL) fatty acids status and its association with plasma Hcy in patients with type 2 diabetes mellitus (T2DM). One hundred and four T2DM (aged 57.3±13.4 y) and 150 healthy subjects (aged 48.4±8.7 y) were recruited. Plasma Hcy and PL fatty acids were determined by standard methods. Plasma Hcy concentration in T2DM was significantly higher than that in healthy subjects (p<0.001). The prevalence of hyperhomocysteinemia was significantly higher in T2DM (36.54%) than that in healthy subjects (17.32%) (p=0.012). Plasma PL 20:4n-6 (r=0.303, p=0.012), 22:5n-3 (r=0.312, p=0.01), total PUFA (r=0.303, p=0.012), n-6 PUFA (r=0.261, p=0.032) were significantly positively associated with plasma Hcy concentration in T2DM. While, plasma PL n-3:n-6 PUFA (r=-0.400, p=0.046) was negatively associated with plasma Hcy in T2DM. In healthy subjects, plasma PL 22:6n-3 (r=-0.201, p=0.042) was negatively associated with plasma Hcy. In addition, plasma PL 22:6n-3 (r=0.193, p=0.044) and 22:5n-6 (r=0.234, p=0.038) were significantly negatively associated with plasma vitamin B-12 in healthy subjects. Our results suggested that increased plasma Hcy levels in T2DM associated with low n-3:n-6 ratio intake. We suggest that T2DM increase their long chain n-3 PUFA intake from fish or fish oil while decrease n-6 PUFA intake. | Adult, Aged, Algorithms, China, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Fatty Acids, Omega-3, Fatty Acids, Omega-6, Fatty Acids, Unsaturated, Female, Folic Acid, Homocysteine, Humans, Hyperhomocysteinemia, Male, Middle Aged, Outpatient Clinics, Hospital, Phospholipids, Prevalence, Vitamin B 12 | null |
22,705,430 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | Assessing current nutritional status of patients with HCV-related liver cirrhosis in the compensated stage. | Yasutake Kenichiro, Bekki Machiko, Ichinose Masako, Ikemoto Michiko, Fujino Tatsuya, Ryu Tomoki, Wada Yoshiyuki, Takami Yuko, Saitsu Hideki, Kohjima Motoyuki, Fukuizumi Kunitaka, Nakashima Manabu, Nakamuta Makoto, Enjoji Munechika | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705430 | Nutritional states of Japanese patients with liver cirrhosis have recently shown great diversity, some show protein energy malnutrition and others excessive nutrition and obesity. For there to be adequate guidance regarding dietary treatment, it is important that a patient's current nutritional state be clarified. | Academic Medical Centers, Aged, Cross-Sectional Studies, Diabetes Complications, Diet, Female, Hepacivirus, Hepatitis C, Hepatitis C, Chronic, Humans, Japan, Liver, Liver Cirrhosis, Male, Malnutrition, Middle Aged, Nutritional Status, Overnutrition, Prevalence, Severity of Illness Index | null |
22,705,431 | 2012-08-30 | 2013-11-21 | 0964-7058 | Asia Pacific journal of clinical nutrition | Evaluation of taste acuity by the filter-paper disc in Japanese young women: the relationship with micronutrients status. | Nagai Ayako, Kubota Masaru, Katayama Yuriko, Kojima Chiaki | eng | null | Journal Article | Dietary Sucrose, Iron, Dietary, Micronutrients, Copper, Selenium, Zinc | IM | 22705431 | The aim of the present study is to investigate the taste acuity in Japanese young women in relation to their micronutrient status. Thirty-eight healthy young women (mean age; 21.3, range; 19-27 years) were enrolled. Gustatory thresholds were estimated for four basic tastes: sweet (sucrose), salty (sodium chloride), sour (tartaric acid), and bitter (quinine hydrochloride) by a filter-paper disk method. Various concentrations at each taste were serially scored from disc number 1 (lowest) to number 5 (highest). The lowest concentration at which the quality of the taste was correctly identified was defined as the recognition threshold. The mean of three measurements for each test on three different days was calculated. We divided our participants into normal taste and hypogeusia groups based on the mean threshold disc numbers, <=3.5 and >3.5, respectively, according to previous literature using the same method. We also measured serum concentrations and dietary intakes of micronutrients including zinc, iron, copper, and selenium. The numbers of participants belonging to the hypogeusia group were 24 (63.2%) for sweet, 19 (50.0%) for sour, 17 (44.7%) for bitter, and 16 (42.1%) for salty taste. Although the hypogeusia group exhibited significantly lower serum iron concentrations, except for the salty taste, the other three micronutrients concentrations did not show any association with the four taste acuities. Dietary micronutrient intake did not show any association with the four taste acuities. This study indicates that in addition to zinc status, iron status should be considered in the study of taste acuity. | Adult, Ageusia, Anemia, Iron-Deficiency, Copper, Diet, Diet Records, Dietary Sucrose, Female, Humans, Iron, Dietary, Japan, Micronutrients, Nutritional Status, Selenium, Taste Threshold, Young Adult, Zinc | null |
22,705,433 | 2012-08-30 | 2022-03-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | High prevalence of anemia with lack of iron deficiency among women in rural Bangladesh: a role for thalassemia and iron in groundwater. | Merrill Rebecca D, Shamim Abu Ahmed, Ali Hasmot, Labrique Alain B, Schulze Kerry, Christian Parul, West Keith P | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Drinking Water, Iron, Dietary, Hemoglobin E, Iron, Arsenic | IM | 22705433 | Iron deficiency was absent in a recent population assessment of rural Bangladeshi women exhibiting anemia (57%), suggesting other causes of low hemoglobin. We assessed the relative influence on anemia of thalassemia, groundwater arsenic and iron, and diet among women of reproductive age living in rural Bangladesh. Women (n=207) sampled from a previous antenatal nutrient intervention trial in rural Bangladesh were visited during two seasons in 2008. Collected data included 7-day dietary and 24-hour drinking water intake recalls, 7-day morbidity recall, anthropometry, and drinking water arsenic and iron concentrations. Capillary blood was analyzed for iron (plasma ferritin, soluble transferrin receptor), inflammation (C-reactive protein) and thalassemia (β thalassemia and Hb E) status. In stratified, adjusted analyses, only parity was associated with anemia (odds ratio, OR (95% CI): 11.34 (1.94, 66.15)) for those with thalassemia (28% prevalent). In contrast, groundwater iron intake (>30 mg/d, 0.48 (0.24, 0.96)) and wasting (2.32 (1.17, 4.62)) were associated with anemia among those without thalassemia. Elevated groundwater arsenic (>50 μg/L, 12% of tubewells) and a diverse diet were unrelated to anemia regardless of thalassemia diagnosis (p>0.70 and >0.47, respectively). Among women in this typical rural Bangladeshi area, the prevalence of thalassemia was high and, unlike iron deficiency which was absent most likely due to high iron intake from groundwater, contributed to the risk of anemia. In such settings, the influence of environmental sources of iron and the role of thalassemias in contributing to anemia at the population level may be underappreciated. | Adult, Anemia, Anemia, Iron-Deficiency, Arsenic, Bangladesh, Diet, Drinking Water, Female, Groundwater, Hemoglobin E, Humans, Iron, Iron, Dietary, Parity, Prevalence, Risk, Rural Health, Thalassemia, Wasting Syndrome, Young Adult | null |
22,705,432 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | Microcytic anaemia predominates in adolescent school girls in the delta region of Myanmar. | Htet Min Kyaw, Dillon Drupadi, Akib Arwin, Utomo Budi, Fahmida Umi, Thurnham David I | eng | null | Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Hemoglobin E | IM | 22705432 | Anaemia is one of major nutritional problems in Myanmar affecting all age groups. However, there is lack of recent information and a study was conducted to acquire information on the current status of anaemia among adolescent schoolgirls in Nyaung Done township, Ayeyarwady division where an intervention study was planned. | Adolescent, Adolescent Development, Anemia, Anemia, Hypochromic, Anemia, Iron-Deficiency, Child, Cross-Sectional Studies, Erythrocyte Indices, Female, Growth Disorders, Hemoglobin E, Hemoglobinopathies, Humans, Myanmar, Prevalence, Wasting Syndrome | null |
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