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22,705,434 | 2012-08-30 | 2022-03-30 | 0964-7058 | Asia Pacific journal of clinical nutrition | Sugary beverage intakes and obesity prevalence among junior high school students in Beijing - a cross-sectional research on SSBs intake. | Jia Meng, Wang Chao, Zhang Yumei, Zheng Yingdong, Zhang Long, Huang Yanjie, Wang Peiyu | eng | null | Journal Article | Dietary Sucrose | IM | 22705434 | Excessive consumption of sugar-sweetened beverages (SSBs) may increase the risk of obesity. Data in regards to the consumption of SSBs is insufficient in the Chinese population, especially in middle school students experiencing rapid nutritional transition. We aimed to describe the consumption of SSBs among junior high school students and explore the relationship between SSB intake and adolescents' overweight/obesity in Beijing. | Adolescent, Adolescent Behavior, Beverages, Body Mass Index, Child, Child Behavior, China, Cross-Sectional Studies, Diet, Dietary Sucrose, Family Health, Female, Humans, Male, Obesity, Overweight, Parents, Prevalence, Sex Characteristics, Urban Health | null |
22,705,435 | 2012-08-30 | 2024-11-27 | 0964-7058 | Asia Pacific journal of clinical nutrition | Lack of folate improvement in high risk indigenous Australian adults over an average of 6.5 years: a cohort study. | D'Onise Katina, McDermott Robyn Anne, Leonard Dympna, Campbell Sandra Kaye | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Folic Acid | IM | 22705435 | Socioeconomically vulnerable groups in developed countries suffer excess chronic disease due in large part to an energy dense but nutrient poor diet. Low folate can be a marker of poor dietary quality and is also affected by smoking and chronic alcohol intake, all of which cluster in groups with a low socioeconomic position. A 4.5 to 9 year follow-up study of 567 indigenous adults from remote communities in far north Queensland, Australia, from 1998 to 2007 was conducted. Analysis of the effects of demographic factors, smoking, risky alcohol drinking, fruit and vegetable intake and waist circumference on changes in red cell folate (RCF) status was conducted. Prevalence of low red cell folate doubled in the cohort from a high baseline over this seven year period: 36.9% deficient in 2007, 15.9% at baseline (p<0.001). Smoking was associated with lower folate levels. People with a normal RCF were less likely to be smokers, and were more likely to have a greater number of serves of vegetables (RR 1.06, 95% CI 1.02-1.10) than those who were deficient at follow-up. The introduction of voluntary folate fortification since 1995 does not appear to have impacted on the already poor folate status of this cohort of adults. The increased prevalence of low folate has occurred despite improvements in the food supply, indicating the need for nutrition promotion, and subsidies for healthy food in remote communities. The impact of mandatory folate fortification of flour since 2009 should be assessed in this high risk population. | Adult, Cohort Studies, Developed Countries, Diet, Erythrocytes, Female, Folic Acid, Folic Acid Deficiency, Follow-Up Studies, Fruit, Health Transition, Humans, Male, Middle Aged, Prevalence, Queensland, Risk, Rural Health, Smoking, Vegetables | null |
22,705,436 | 2012-08-30 | 2024-12-05 | 0964-7058 | Asia Pacific journal of clinical nutrition | Folate knowledge and consumer behaviour among pregnant New Zealand women prior to the potential introduction of mandatory fortification. | Mallard Simonette R, Houghton Lisa A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Folic Acid | IM | 22705436 | To reduce the risk of neural tube defects, the New Zealand Ministry of Health recommends women take supplemental folic acid from at least one month preconception until the end of the twelfth week of pregnancy, as well as consume folate-rich foods. A postpartum survey was conducted to describe folate knowledge and consumer behaviour among pregnant New Zealand women prior to the potential implementation of mandatory folic acid fortification of bread in May 2012. Increasing knowledge of folic acid recommendations was associated with higher supplement uptake among women who planned their pregnancies (p=0.001 for linear trend). Folic acid information failed to adequately reach some socio-demographic subgroups before conception, even when pregnancy was planned, including: indigenous Maori, Pacific and Asian women, younger women, women with large families, and women with lower educational attainment and income. Only half of all women surveyed knew some bread contained added folic acid, and among these women, less than 2% consistently chose voluntarily fortified bread during the periconceptional period by inspecting labels. Sixty-one percent of women indicated they were either in favour of mandatory fortification, or held no opinion on the matter, while 4% were opposed to the addition of folic acid to bread. Approximately one-third (35%) of women agreed with voluntary fortification. Future health promotion initiatives should be tailored toward women who are younger, less educated, with lower income, multiparous or of minority ethnicity status. Nonetheless, mandatory folic acid fortification may be required to attain the desired degree of equity. | Adult, Bread, Consumer Health Information, Diet, Dietary Supplements, Female, Folic Acid, Food, Fortified, Health Behavior, Health Knowledge, Attitudes, Practice, Health Promotion, Humans, Neural Tube Defects, New Zealand, Nutrition Surveys, Nutritional Sciences, Postpartum Period, Pregnancy, Young Adult | null |
22,705,437 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | Food group intake among adolescents in Guangzhou city compared with the Chinese dietary guidelines. | Zhang Cai-Xia, Chen Yu-Ming, Chen Wei-Qing, Su Yi-Xiang, Wang Cui-Ling, Wu Jiang-Nan | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705437 | Food group intake in adolescents has many health implications. However, no study has been conducted to assess food group intake among Chinese adolescents. | Adolescent, Adolescent Behavior, Adolescent Development, Child, China, Cross-Sectional Studies, Diet, Female, Food, Health Behavior, Health Knowledge, Attitudes, Practice, Health Promotion, Humans, Male, Nutrition Policy, Nutrition Surveys, Sex Characteristics, Urban Health | null |
22,705,439 | 2012-08-30 | 2012-06-18 | 0964-7058 | Asia Pacific journal of clinical nutrition | High output enterocutaneous fistula: a literature review and a case study. | Tong Chung Yan, Lim Li Lin, Brody Rebecca A | eng | null | Case Reports, Journal Article, Review | Anti-Bacterial Agents | IM | 22705439 | An enterocutaneous (EC) fistula is referred to as a channel between the gut and the skin. Effluent of an EC fistula of more than 500 ml per day is considered as high output. Patients with high output EC fistulae have a high morbidity and mortality rate. No evidence-based guidelines are available for this condition and more research is required to evaluate the effectiveness of treatment. Nevertheless, patients with fistulae should be managed based on the available evidence, detailed clinical and nutrition assessment, and close monitoring. Management of high output EC fistula is complex and challenging. It involves nutrition, medical, skin care and psychological treatment, which is best managed by a multidisciplinary team. It requires an individualized nutrition and clinical treatment plan to maximize patient outcomes. Up to 70% of patients with fistulae have malnutrition and it is a significant prognostic factor of spontaneous fistula closure. Nutrition therapies including macronutrient and micronutrient delivery, enteral nutrition and parenteral nutrition are discussed in this review. A case study of a patient with multiple EC fistulae is presented to illustrate the management of high output EC fistulae. | Adult, Anti-Bacterial Agents, Female, Humans, Intestinal Fistula, Intraabdominal Infections, Parenteral Nutrition, Postoperative Complications, Sepsis, Severity of Illness Index, Treatment Outcome, Young Adult | null |
22,705,438 | 2012-08-30 | 2022-12-07 | 0964-7058 | Asia Pacific journal of clinical nutrition | Association between insulin receptor gene polymorphism and the metabolic syndrome in Han and Yi Chinese. | Wang Chunxiu, Wang Bin, He Huijing, Li Xiaotong, Wei Daying, Zhang Jianhua, Ma Mingju, Pan Li, Yu Tao, Xue Fang, Li Lin, Shan Guangliang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antigens, CD, INSR protein, human, Receptor, Insulin | IM | 22705438 | Insulin resistance has been a possible underlying pathophysiologic defect inducing the metabolic syndrome (MS). However association studies regarding Insulin receptor gene in different ethnic groups are scarce in literature. Here we conduct an association study between MS and genetic polymorphism of the INSR gene in Yi and Han Chinese. In a cross-sectional study, 3,436 Yi and Han people were investigated. Ethnicity-specific case-control studies were designed, with MS patients diagnosed as cases and non-MS people as controls matched on gender and age. Polymerase chain reaction-restriction fragment length polymorphism was used to detect the genotypes of the exon 8 of the INSR gene. Data were analyzed using one-way analysis of variance, chi-square test, and logistic regression where appropriate. Systolic blood pressure (SBP) was significantly higher in MS patients with the N₁N₂/N₂N₂ genotypes than that in those with the N₁N₁ genotype of both ethnic population (p<0.05). Frequency of the N₂ allele was significantly higher in MS patients than that in controls of ethnic Han (p=0.020). Multivariable logistic regression analysis showed that the NsiI polymorphism of the exon 8 of the INSR was an independent predictor for MS in Han people adjusted for total cholesterol, sex, physical activity, educational level, family income, alcohol intake and smoking (OR=2.55, 95% CI: 1.31-4.94, p=0.006). The results indicated that NsiI polymorphism of the INSR gene is associated with SBP in these two different ethnic groups, and significantly associate with MS in Han Chinese. These findings contribute to our better understanding on the genetic basis of MS. | Adult, Aged, Aged, 80 and over, Alleles, Antigens, CD, Asian People, Case-Control Studies, China, Cross-Sectional Studies, Exons, Female, Gene Frequency, Genetic Association Studies, Humans, Hypertension, Male, Metabolic Syndrome, Middle Aged, Polymorphism, Genetic, Receptor, Insulin, Young Adult | null |
22,705,440 | 2013-02-15 | 2017-11-16 | 0006-3002 | Biochimica et biophysica acta | Peroxisome biogenesis disorders: molecular basis for impaired peroxisomal membrane assembly: in metabolic functions and biogenesis of peroxisomes in health and disease. | Fujiki Yukio, Yagita Yuichi, Matsuzaki Takashi | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Lipoproteins, Membrane Proteins, PEX16 protein, human, Peroxins, Pex3 protein, human, PEX19 protein, human | IM | 22705440, S0925-4439(12)00138-X, 10.1016/j.bbadis.2012.06.004 | Peroxisome is a single-membrane organelle in eukaryotes. The functional importance of peroxisomes in humans is highlighted by peroxisome-deficient peroxisome biogenesis disorders (PBDs) such as Zellweger syndrome (ZS). Gene defects of peroxins required for both membrane assembly and matrix protein import are identified: ten mammalian pathogenic peroxins for ten complementation groups of PBDs, are required for matrix protein import; three, Pex3p, Pex16p and Pex19p, are shown to be essential for peroxisome membrane assembly and responsible for the most severe ZS in PBDs of three complementation groups 12, 9, and 14, respectively. Patients with severe ZS with defects of PEX3, PEX16, and PEX19 tend to carry severe mutation such as nonsense mutations, frameshifts and deletions. With respect to the function of these three peroxins in membrane biogenesis, two distinct pathways have been proposed for the import of peroxisomal membrane proteins in mammalian cells: a Pex19p- and Pex3p-dependent class I pathway and a Pex19p- and Pex16p-dependent class II pathway. In class II pathway, Pex19p also forms a soluble complex with newly synthesized Pex3p as the chaperone for Pex3p in the cytosol and directly translocates it to peroxisomes. Pex16p functions as the peroxisomal membrane receptor that is specific to the Pex3p-Pex19p complexes. A model for the import of peroxisomal membrane proteins is suggested, providing new insights into the molecular mechanisms underlying the biogenesis of peroxisomes and its regulation involving Pex3p, Pex19p, and Pex16p. Another model suggests that in Saccharomyces cerevisiae peroxisomes likely emerge from the endoplasmic reticulum. | Animals, Genetic Heterogeneity, Health, Humans, Intracellular Membranes, Lipoproteins, Membrane Proteins, Mutation, Peroxins, Peroxisomal Disorders, Peroxisomes | null |
22,705,441 | 2012-11-21 | 2021-10-21 | 1873-3514 | Neuropsychologia | Memorial familiarity remains intact for pictures but not for words in patients with amnestic mild cognitive impairment. | Embree Lindsay M, Budson Andrew E, Ally Brandon A | eng | AG013846 (NIA NIH HHS, United States); P30 AG013846 (NIA NIH HHS, United States); K23 AG031925 (NIA NIH HHS, United States); AG038471 (NIA NIH HHS, United States); AG025815 (NIA NIH HHS, United States); AG031925 (NIA NIH HHS, United States); R01 AG038471 (NIA NIH HHS, United States); R01 AG025815 (NIA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22705441, S0028-3932(12)00239-4, 10.1016/j.neuropsychologia.2012.06.001, PMC3399023, NIHMS390197, 20727943, 11849301, 17207639, 9621828, 17425893, 15250777, 15450686, 12374325, 19101064, 21946012, 18999344, 1202204, 16594780, 11204105, 1573197, 2771064, 17481940, 19467355, 21620877, 16009248, 9560154, 15324362, 1502275, 15705615, 18400793, 18263627, 17981307, 20362596, 16060779, 14732623, 7983467, 16594795, 17723031, 11949708, 18328509, 10855417, 21775732, 6457080, 1449404, 16479105, 16550852, 2966230, 17475539, 20882548, 15975947, 19996875, 3670049, 20032154, 20350169, 22229341, 11204095, 8901341, 9673991, 10946380, 18331160, 18992266, 19250730, 11394676, 1003125, 11209950, 19467356 | Understanding how memory breaks down in the earliest stages of Alzheimer's disease (AD) process has significant implications, both clinically and with respect to intervention development. Previous work has highlighted a robust picture superiority effect in patients with amnestic mild cognitive impairment (aMCI). However, it remains unclear as to how pictures improve memory compared to words in this patient population. In the current study, we utilized receiver operating characteristic (ROC) curves to obtain estimates of familiarity and recollection for pictures and words in patients with aMCI and healthy older controls. Analysis of accuracy shows that even when performance is matched between pictures and words in the healthy control group, patients with aMCI continue to show a significant picture superiority effect. The results of the ROC analysis showed that patients demonstrated significantly impaired recollection and familiarity for words compared controls. In contrast, patients with aMCI demonstrated impaired recollection, but intact familiarity for pictures, compared to controls. Based on previous work from our lab, we speculate that patients can utilize the rich conceptual information provided by pictures to enhance familiarity, and perceptual information may allow for post-retrieval monitoring or verification of the enhanced sense of familiarity. Alternatively, the combination of enhanced conceptual and perceptual fluency of the test item might drive a stronger or more robust sense of familiarity that can be accurately attributed to a studied item. | Aged, Amnesia, Cognitive Dysfunction, Female, Humans, Male, Memory, Mental Recall, Neuropsychological Tests, Photic Stimulation, Psychomotor Performance, ROC Curve, Reading, Recognition, Psychology, Reproducibility of Results, Trail Making Test, Visual Perception | null |
22,705,442 | 2012-11-21 | 2024-06-10 | 1873-3514 | Neuropsychologia | Memory monitoring performance and PFC activity are associated with 5-HTTLPR genotype in older adults. | Pacheco Jennifer, Beevers Christopher G, McGeary John E, Schnyer David M | eng | R99 AG999999 (NIA NIH HHS, United States); S10 RR023457 (NCRR NIH HHS, United States); Z99 AG999999 (Intramural NIH HHS, United States) | Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | SLC6A4 protein, human, Serotonin Plasma Membrane Transport Proteins, DNA | IM | 22705442, S0028-3932(12)00232-1, 10.1016/j.neuropsychologia.2012.05.030, PMC3400137, NIHMS386741, 18061310, 16989546, 16011544, 16303318, 16710715, 9210796, 15788702, 16093315, 8852732, 19035823, 2505291, 17598730, 14766185, 19439600, 17353910, 14566344, 12414279, 12185399, 19634035, 6544432, 17328371, 15654286, 8987764, 11014708, 21463057, 17385989, 12061414, 12457268, 18394488, 17201560, 11584878, 18221797, 10385578, 12372291, 11870007, 15050513, 12130784, 16725162, 12217177, 12869766, 11533730, 21616984, 15887056, 16054861, 15584793, 11707089, 17675110, 14998710, 17114303, 3502895, 12537035, 19586165, 9189980, 20183099, 21680847, 12367516, 15383004, 20599283, 16158065, 16774962, 1594723, 19396166, 20039945, 16846860, 12764084, 15028641, 9100268, 11931290, 17364379, 15831406, 20604613, 12061407, 19290745, 19847066, 15904549, 8831298, 2803603, 7485180, 12725759, 18757724, 9421570, 17585888, 2898042 | Older adults show extensive variability in cognitive performance, including episodic memory. A portion of this variability could potentially be explained by genetic factors. Recent literature shows that the neurotransmitter serotonin plays an important role in memory processes, as enhancements of brain serotonin have led to memory improvement. Here, we have begun to explore genetic contributions to the performance and underlying brain activity associated with source memory monitoring. Using a source recognition memory task during fMRI scanning, this study offers evidence that older adults who carry a short allele (S-car) of the serotonin transporter linked polymorphic region (5-HTTLPR) in the SLC6A4 gene show specific deficits in source memory monitoring relative to older adults who are homozygous for the long allele (LL). These deficits are accompanied by less neural activity in regions of prefrontal cortex that have been shown to support accurate memory monitoring. Moreover, while the older adult LL group's behavioral performance does not differ from younger adults, their brain activation reveals evidence of compensatory activation that likely supports their higher performance level. These results provide preliminary evidence that the long-allele homozygous profile is cognitively beneficial to older adults, particularly for memory functioning. | Adult, Aged, Aged, 80 and over, Aging, Alleles, Analysis of Variance, DNA, Female, Genotype, Homozygote, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Memory, Middle Aged, Prefrontal Cortex, Psychomotor Performance, Reaction Time, Serotonin Plasma Membrane Transport Proteins, Young Adult | null |
22,705,447 | 2013-01-09 | 2012-08-21 | 1095-9564 | Neurobiology of learning and memory | Learning strategy is influenced by trait anxiety and early rearing conditions in prepubertal male, but not prepubertal female rats. | Grissom Elin M, Hawley Wayne R, Bromley-Dulfano Sarah S, Marino Sarah E, Stathopoulos Nicholas G, Dohanich Gary P | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705447, S1074-7427(12)00076-7, 10.1016/j.nlm.2012.06.001 | Rodents solve dual-solution tasks that require navigation to a goal by adopting either a hippocampus-dependent place strategy or a striatum-dependent stimulus-response strategy. A variety of factors, including biological sex and emotional status, influence the choice of learning strategy. In these experiments, we investigated the relationship between learning strategy and anxiety level in male and female rats prior to the onset of puberty, before the activational effects of gonadal hormones influence these processes. In the first experiment, prepubertal male rats categorized as high in trait anxiety at 26days of age exhibited a bias toward stimulus-response strategy at 28days of age, whereas age-matched females exhibited no preference in strategy regardless of anxiety level. In the second experiment, male and female rats were separated from their dams for either 15 or 180min per day during the first 2weeks of life and tested on a battery of anxiety and cognitive tasks between 25 and 29days of age. Prolonged maternal separations for 180min were associated with impaired spatial memory on a Y-maze task in both prepubertal males and females. Furthermore, prolonged maternal separations were linked to elevated anxiety and a bias for stimulus-response strategy in prepubertal males but not females. Alternatively, brief separations from dams for 15min were associated with intact spatial memory, lower levels of anxiety, and no preference for either learning strategy in both sexes. These results provide evidence of sex-specific effects of trait anxiety and early maternal separation on the choice of learning strategy used by prepubertal rodents. | Animals, Anxiety, Choice Behavior, Female, Hippocampus, Learning, Male, Maternal Deprivation, Maze Learning, Memory, Rats, Sex Factors, Spatial Behavior | null |
22,705,448 | 2013-02-06 | 2021-10-21 | 1095-6859 | Gynecologic oncology | Pair Box 8 (PAX8) protein expression in high grade, late stage (stages III and IV) ovarian serous carcinoma. | Mhawech-Fauceglia Paulette, Wang Dan, Samrao Damanzoopinder, Godoy Heidi, Ough Faith, Liu Song, Pejovic Tanja, Lele Shashikant | eng | T32 CA108456 (NCI NIH HHS, United States) | Journal Article | Biomarkers, Tumor, PAX8 Transcription Factor, PAX8 protein, human, Paired Box Transcription Factors | IM | 22705448, S0090-8258(12)00450-7, 10.1016/j.ygyno.2012.06.012, PMC5049509, NIHMS818900, 22348438, 21746896, 17082261, 15087669, 9662379, 17704411, 17064757, 17273775, 12860964, 19053170, 20414098, 21552115, 17397910, 15554941, 17018256, 18724243 | Pair-Box 8 (PAX8) is a transcription factor which has been found to be overexpressed in ovarian serous carcinoma (OSC). Silencing PAX8 by using shRNA led to a drop in cell viability in ovarian cancer cell lines, suggesting its use as a targeted therapeutic agent. The prognostic value of PAX8 in OSC is still widely unknown. The aim of this study was to evaluate PAX8 as a prognostic biomarker in patients with advanced stage OSC. | Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Carcinoma, Ovarian Epithelial, Cystadenocarcinoma, Serous, Female, Humans, Immunohistochemistry, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Neoplasms, Glandular and Epithelial, Ovarian Neoplasms, PAX8 Transcription Factor, Paired Box Transcription Factors | null |
22,705,449 | 2013-04-08 | 2015-11-19 | 1873-2933 | Clinical biochemistry | The prognostic value of serum tau in patients with intracerebral hemorrhage. | Hu Hai-Tao, Xiao Feng, Yan Yu-Qing, Wen Shu-Qun, Zhang Liang | eng | null | Journal Article | Biomarkers, MAPT protein, human, tau Proteins | IM | 22705449, S0009-9120(12)00272-X, 10.1016/j.clinbiochem.2012.06.003 | This study aimed to investigate the relationship between serum tau concentrations and 3-month clinical outcomes in patients with intracerebral hemorrhage. | Aged, Area Under Curve, Biomarkers, Cerebral Hemorrhage, Female, Humans, Logistic Models, Male, Middle Aged, Prognosis, ROC Curve, tau Proteins | null |
22,705,446 | 2013-04-12 | 2021-10-21 | 1879-0429 | Current opinion in biotechnology | Material strategies for creating artificial cell-instructive niches. | Edalat Faramarz, Sheu Iris, Manoucheri Sam, Khademhosseini Ali | eng | RL1 DE019024-03 (NIDCR NIH HHS, United States); R01 EB012597 (NIBIB NIH HHS, United States); HL092836 (NHLBI NIH HHS, United States); EB012597 (NIBIB NIH HHS, United States); DE019024 (NIDCR NIH HHS, United States); R01 HL092836 (NHLBI NIH HHS, United States); EB008392 (NIBIB NIH HHS, United States); DE021468 (NIDCR NIH HHS, United States); R01 DE021468 (NIDCR NIH HHS, United States); HL099073 (NHLBI NIH HHS, United States); R01 AR057837 (NIAMS NIH HHS, United States); R01 HL099073 (NHLBI NIH HHS, United States); AR057837 (NIAMS NIH HHS, United States); RL1 DE019024 (NIDCR NIH HHS, United States); R01 EB008392 (NIBIB NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S., Review | Biocompatible Materials, Cytokines | IM | 22705446, S0958-1669(12)00090-0, 10.1016/j.copbio.2012.05.007, PMC3534967, NIHMS382203, 11433435, 16622893, 17237789, 22521491, 20537705, 20450216, 19424448, 22437210, 20882497, 16293750, 20418863, 19795385, 21819114, 20576850, 20890282, 18281090, 19820164, 18950232, 22101755, 22105780, 19197316, 20543851, 22117858, 20091874, 20882499, 21149682, 21571635, 18193059, 18396331, 19246488, 20647425, 19816774, 19071168, 21265021, 22907987, 19472329, 19495458, 22434367, 21492931, 17582636, 21892177, 15364962, 17435762, 11721046, 21306888, 20795703, 22094245, 20882500, 19415467, 20303169, 21808045, 18978786, 21344866, 18838160, 21800912, 19481080, 22265786, 19500832, 20493521, 22108911, 22223475, 20576885, 21887125, 22068594 | There has been a tremendous growth in the use of biomaterials serving as cellular scaffolds for tissue engineering applications. Recently, advanced material strategies have been developed to incorporate structural, mechanical, and biochemical signals that can interact with the cell and the in vivo environment in a biologically specific manner. In this article, strategies such as the use of composite materials and material processing methods to better mimic the extracellular matrix, integration of mechanical and topographical properties of materials in scaffold design, and incorporation of biochemical cues such as cytokines in tethered, soluble, or time-released forms are presented. Finally, replication of the dynamic forces and biochemical gradients of the in vivo cellular environment through the use of microfluidics is highlighted. | Animals, Artificial Cells, Biocompatible Materials, Cellular Microenvironment, Cytokines, Humans, Microfluidics, Tissue Engineering, Tissue Scaffolds | null |
22,705,451 | 2013-01-23 | 2022-12-07 | 1873-2933 | Clinical biochemistry | HbA(1c)--an analyte of increasing importance. | Higgins Trefor | eng | null | Journal Article, Review | Blood Glucose, Glycated Hemoglobin A | IM | 22705451, S0009-9120(12)00275-5, 10.1016/j.clinbiochem.2012.06.006 | Since the incorporation in 1976 of HbA(1c) into a monitoring program of individuals with diabetes, this test has become the gold standard for assessment of glycemic control. Analytical methods have steadily improved in the past two decades, largely through the efforts of the National Glycohemoglobin Standardization program (NGSP). The new definition of HbA(1c) and the introduction of an analytically pure calibrator have increased the possibility for greater improvements in analytical performance. Controversies exist in the reporting of HbA(1c). The use of HbA(1c) has expanded beyond the use solely as a measure of glycemic control into a test for screening and diagnosing diabetes. With improvements in analytical performance, the effects of demographic factors such as age and ethnicity and clinical factors such as iron deficiency have been observed. In this review, the history, formation, analytical methods and parameters that affect HbA(1c) analysis are discussed. | Age Factors, Anemia, Iron-Deficiency, Blood Chemical Analysis, Blood Glucose, Chemistry Techniques, Analytical, Diabetes Complications, Diabetes Mellitus, Disease Progression, Glycated Hemoglobin, Glycemic Index, Humans, Reference Values, Risk Factors | null |
22,705,450 | 2013-04-08 | 2022-04-08 | 1873-2933 | Clinical biochemistry | Pathophysiology, clinics, diagnosis and treatment of heart involvement in carbon monoxide poisoning. | Lippi Giuseppe, Rastelli Gianni, Meschi Tiziana, Borghi Loris, Cervellin Gianfranco | eng | null | Journal Article, Review | Carbon Monoxide | IM | 22705450, S0009-9120(12)00273-1, 10.1016/j.clinbiochem.2012.06.004 | The toxicity of carbon monoxide has been recognized for long throughout history and is unquestionably the leading cause of unintentional poisoning deaths in the Western countries. The severity of poisoning is dependent upon environmental and human factor. The leading pathophysiological mechanism resides in the ability of carbon monoxide to bind to hemoglobin molecules with high affinity, displacing oxygen and generating carboxyhemoglobin, which is virtually ineffective to deliver oxygen to the tissues. The organs with the highest demand for oxygen such as the brain and the heart are more vulnerable to injury. Myocardial involvement is commonplace in moderate to severe carbon monoxide poisoning and is associated with a substantially higher risk of mortality. Besides hypoxic damage, carbon monoxide produces myocardium injuries with cardiospecific mechanisms, mostly attributable to direct damage at cellular or subcellular level. The clinical spectrum of heart involvement is broad and encompasses cardiomyopathy, angina attack, myocardial infarction, arrhythmias and heart failure up to myocardial stunning, cardiogenic shock and sudden death. Patients with underlying cardiac disease, especially coronary heart disease, are at greater risk of infarction and arrhythmias. Single photon emission computed tomography (SPECT) is the technique of choice for diagnosing cardiac involvement, whereas the recent introduction of the highly sensitive troponin immunoassays seems promising for the early triage of patients. No specific treatment other than oxygen delivery can be advocated for cardiac toxicity at present, and 100% oxygen therapy should be continued until the patient is asymptomatic and carboxyhemoglobin levels decrease below 5-10%. | Animals, Carbon Monoxide, Carbon Monoxide Poisoning, Heart Diseases, Humans, Hyperbaric Oxygenation, Hypoxia | null |
22,705,452 | 2014-02-26 | 2013-07-03 | 1460-2199 | Cerebral cortex (New York, N.Y. : 1991) | PTB deficiency causes the loss of adherens junctions in the dorsal telencephalon and leads to lethal hydrocephalus. | Shibasaki Takayuki, Tokunaga Akinori, Sakamoto Reiko, Sagara Hiroshi, Noguchi Shigeru, Sasaoka Toshikuni, Yoshida Nobuaki | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Polypyrimidine Tract-Binding Protein | IM | 22705452, bhs161, 10.1093/cercor/bhs161 | Polypyrimidine tract-binding protein (PTB) is a well-characterized RNA-binding protein and known to be preferentially expressed in neural stem cells (NSCs) in the central nervous system; however, its role in NSCs in the developing brain remains unclear. To explore the role of PTB in embryonic NSCs in vivo, Nestin-Cre-mediated conditional Ptb knockout mice were generated for this study. In the mutant forebrain, despite the depletion of PTB protein, neither abnormal neurogenesis nor flagrant morphological abnormalities were observed at embryonic day 14.5 (E14.5). Nevertheless, by 10 weeks, nearly all mutant mice succumbed to hydrocephalus (HC), which was caused by a lack of the ependymal cell layer in the dorsal cortex. Upon further analysis, a gradual loss of adherens junctions (AJs) was observed in the ventricular zone (VZ) of the dorsal telencephalon in the mutant brains, beginning at E14.5. In the AJs-deficient VZ, impaired interkinetic nuclear migration and precocious differentiation of NSCs were observed after E14.5. These findings demonstrated that PTB depletion in the dorsal telencephalon is causally involved in the development of HC and that PTB is important for the maintenance of AJs in the NSCs of the dorsal telencephalon. | Adherens Junctions, Animals, Hydrocephalus, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Neural Stem Cells, Polypyrimidine Tract-Binding Protein, Telencephalon | developing brain, ependymal cells, neural stem cell, precocious differentiation, region specific |
22,705,443 | 2013-02-15 | 2012-08-27 | 1872-9452 | Molecular aspects of medicine | Foreword to the special issue on water channel proteins (aquaporins and relatives) in health and disease: 25 years after the discovery of the first water channel protein, later called aquaporin 1. | Benga Gheorghe | eng | null | Historical Article, Introductory Journal Article | Aquaporins, Aquaporin 1 | IM | 22705443, S0098-2997(12)00062-3, 10.1016/j.mam.2012.06.002 | null | Animals, Aquaporin 1, Aquaporins, History, 20th Century, History, 21st Century, Humans, Terminology as Topic | null |
22,705,453 | 2012-11-23 | 2012-07-24 | 1873-5118 | Progress in neurobiology | TrkB inhibition as a therapeutic target for CNS-related disorders. | Boulle Fabien, Kenis Gunter, Cazorla Maxime, Hamon Michel, Steinbusch Harry W M, Lanfumey Laurence, van den Hove Daniel L A | eng | null | Journal Article, Review | Brain-Derived Neurotrophic Factor, Receptor, trkB | IM | 22705453, S0301-0082(12)00100-1, 10.1016/j.pneurobio.2012.06.002 | The interaction of brain-derived neurotrophic factor (BDNF) with its tropomyosin-related kinase receptor B (TrkB) is involved in fundamental cellular processes including neuronal proliferation, differentiation and survival as well as neurotransmitter release and synaptic plasticity. TrkB signaling has been widely associated with beneficial, trophic effects and many commonly used psychotropic drugs aim to increase BDNF levels in the brain. However, it is likely that a prolonged increased TrkB activation is observed in many pathological conditions, which may underlie the development and course of clinical symptoms. Interestingly, genetic and pharmacological studies aiming at decreasing TrkB activation in rodent models mimicking human pathology have demonstrated a promising therapeutic landscape for TrkB inhibitors in the treatment of various diseases, e.g. central nervous system (CNS) disorders and several types of cancer. Up to date, only a few selective and potent TrkB inhibitors have been developed. As such, the use of crystallography and in silico approaches to model BDNF-TrkB interaction and to generate relevant pharmacophores represent powerful tools to develop novel compounds targeting the TrkB receptor. | Animals, Brain, Brain-Derived Neurotrophic Factor, Central Nervous System Diseases, Humans, Receptor, trkB, Signal Transduction | null |
22,705,444 | 2012-10-31 | 2021-10-21 | 1872-9452 | Molecular aspects of medicine | Animal models of age related macular degeneration. | Pennesi Mark E, Neuringer Martha, Courtney Robert J | eng | P51 RR000163 (NCRR NIH HHS, United States); K08 EY021186 (NEI NIH HHS, United States); K01 RR000163 (NCRR NIH HHS, United States); RR-00163 (NCRR NIH HHS, United States); 1 K08 EY021186-01 (NEI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review | null | IM | 22705444, S0098-2997(12)00063-5, 10.1016/j.mam.2012.06.003, PMC3770531, NIHMS511618, 12359593, 17065470, 15944936, 15824225, 16634936, 7548618, 11950240, 11879138, 12600915, 11238064, 19028492, 17921253, 12567264, 15761120, 3574673, 18696097, 18806290, 9342361, 15870199, 10342375, 8433826, 12789138, 17616779, 15761122, 19416113, 7687813, 14662593, 21287191, 15895326, 17219108, 11721184, 21051730, 1423598, 9218423, 15555528, 16844785, 20157514, 10213241, 12972764, 9988272, 19255449, 10942527, 8859077, 21690377, 16396647, 17615538, 7418739, 16299144, 21357400, 10880384, 21098885, 10699158, 16849499, 17921404, 22205601, 18709381, 8419462, 21296820, 1739683, 16905558, 18372426, 19560552, 2605144, 11040088, 16352919, 10892840, 15309487, 7755360, 12939290, 7683682, 15006849, 16123398, 21457267, 12882810, 10488949, 9893599, 7428841, 9046260, 11920687, 12368198, 11173253, 8921239, 18536949, 18190458, 12391305, 3401135, 11133878, 20206286, 18781316, 1692312, 10852960, 2417981, 20868767, 14566334, 15500819, 17909628, 9437322, 11297486, 2713310, 2789127, 21731737, 4004621, 14714098, 17634448, 19822850, 20813971, 18326691, 12799274, 12657599, 9438577, 18195228, 15180266, 16572190, 16023893, 2536645, 16123436, 12652651, 10942325, 11839592, 8702796, 11850530, 10433935, 12506102, 6192474, 1714270, 18326747, 2457955, 17249563, 16452172, 12605267, 11709015, 19661236, 18752525, 10440240, 7224938, 15671300, 10485908, 10865992, 19120017, 16732273, 21967227, 9811357, 10714678, 8690030, 11527927, 12923198, 7999021, 22447858, 16129096, 12368224, 15611275, 1279270, 21454496, 16079201, 21051716, 21851170, 2194475, 12147610, 11594942, 21126357, 10362799, 8983973, 20538999, 19450445, 15975825, 21191149, 11979237, 11006256, 19211094, 20538989, 12634111, 21347253, 19996827, 15180251, 14638737, 12747649, 21414311, 9634502, 12091909, 12014844, 8218037, 16910860, 10532441, 17767156, 15300107, 7309433, 18781584, 15277509, 2581536, 7786215, 11527956, 18535016, 12884119, 8135816, 18682809, 9176680, 94717, 20221855, 9088907, 18172114, 100467, 2402402, 19111720, 18223656, 7976831, 3721502, 19585363, 7700885, 10562651, 17652758, 16518403, 15234127, 19158960, 17898259, 17502507, 21574814, 952802, 17962472, 7822146, 19324038, 15761121, 15374284, 17846362, 17368622, 3492917, 12457866, 16431974, 19578022, 21526923, 20382825, 16600942, 11090485, 11231773, 3423437, 20395434, 7692366, 16099945, 15365174, 9463410, 18288886, 12151537, 21979047, 12601052, 8827966, 20393113, 17372852, 2464985, 18308304, 1411543, 7521577, 6182868, 16503771, 10805752, 15111582, 6204261, 15749821, 7667310, 11053297, 21386905, 10783137, 16579984, 9501855, 15473063, 1558013, 21851472, 17890782, 10674708, 18421225, 1691475, 20195625, 12037010, 7409981, 17664227, 16325179, 10721957, 11124296, 14691182, 931687, 16151432, 20538655, 20457981, 15610977, 7493016, 17122098, 10412977, 12556414, 12657621, 16467679, 15208270, 15389527, 20488255 | Age related macular degeneration (AMD) is the leading cause of vision loss of those over the age of 65 in the industrialized world. The prevalence and need to develop effective treatments for AMD has lead to the development of multiple animal models. AMD is a complex and heterogeneous disease that involves the interaction of both genetic and environmental factors with the unique anatomy of the human macula. Models in mice, rats, rabbits, pigs and non-human primates have recreated many of the histological features of AMD and provided much insight into the underlying pathological mechanisms of this disease. In spite of the large number of models developed, no one model yet recapitulates all of the features of human AMD. However, these models have helped reveal the roles of chronic oxidative damage, inflammation and immune dysregulation, and lipid metabolism in the development of AMD. Models for induced choroidal neovascularization have served as the backbone for testing new therapies. This article will review the diversity of animal models that exist for AMD as well as their strengths and limitations. | Animals, Disease Models, Animal, Humans, Macular Degeneration, Mice, Primates, Rabbits, Rats, Swine | null |
22,705,445 | 2013-02-15 | 2013-11-21 | 1872-9452 | Molecular aspects of medicine | The first discovered water channel protein, later called aquaporin 1: molecular characteristics, functions and medical implications. | Benga Gheorghe | eng | null | Journal Article, Review | Gases, Protein Subunits, Water, Aquaporin 1 | IM | 22705445, S0098-2997(12)00061-1, 10.1016/j.mam.2012.06.001 | After a decade of work on the water permeability of red blood cells (RBC) Benga group in Cluj-Napoca, Romania, discovered in 1985 the first water channel protein in the RBC membrane. The discovery was reported in publications in 1986 and reviewed in subsequent years. The same protein was purified by chance by Agre group in Baltimore, USA, in 1988, who called in 1991 the protein CHIP28 (CHannel forming Integral membrane Protein of 28 kDa), suggesting that it may play a role in linkage of the membrane skeleton to the lipid bilayer. In 1992 the Agre group identified CHIP28's water transport property. One year later CHIP28 was named aquaporin 1, abbreviated as AQP1. In this review the molecular structure-function relationships of AQP1 are presented. In the natural or model membranes AQP1 is in the form of a homotetramer, however, each monomer has an independent water channel (pore). The three-dimensional structure of AQP1 is described, with a detailed description of the channel (pore), the molecular mechanisms of permeation through the channel of water molecules and exclusion of protons. The permeability of the pore to gases (CO(2), NH(3), NO, O(2)) and ions is also mentioned. I have also reviewed the functional roles and medical implications of AQP1 expressed in various organs and cells (microvascular endothelial cells, kidney, central nervous system, eye, lacrimal and salivary glands, respiratory apparatus, gastrointestinal tract, hepatobiliary compartments, female and male reproductive system, inner ear, skin). The role of AQP1 in cell migration and angiogenesis in relation with cancer, the genetics of AQP1 and mutations in human subjects are also mentioned. The role of AQP1 in red blood cells is discussed based on our comparative studies of water permeability in over 30 species. | Animals, Aquaporin 1, Biological Transport, Cell Membrane Permeability, Erythrocyte Membrane, Erythrocytes, Gases, Gene Expression, Humans, Metabolic Diseases, Models, Molecular, Mutation, Protein Multimerization, Protein Subunits, Water | null |
22,705,456 | 2013-07-22 | 2013-02-11 | 1872-6968 | Clinical neurology and neurosurgery | Survival of brain metastatic patients treated with gamma knife radiosurgery alone. | Chiou Shang-Ming | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705456, S0303-8467(12)00278-8, 10.1016/j.clineuro.2012.05.018 | To evaluate the impact of gamma knife radiosurgery (GKRS) alone on the survival of brain metastasis patients. | Adult, Aged, Aged, 80 and over, Brain Neoplasms, Breast Neoplasms, Cohort Studies, Data Interpretation, Statistical, Disease-Free Survival, Female, Humans, Kaplan-Meier Estimate, Karnofsky Performance Status, Lung Neoplasms, Male, Middle Aged, Prognosis, Radiosurgery, Survival Analysis, Treatment Outcome | null |
22,705,457 | 2013-03-28 | 2018-12-01 | 1872-6968 | Clinical neurology and neurosurgery | Extending thrombolytic therapy beyond 6 h: which 'mismatch' are you talking about? | Mattei Tobias A | eng | null | Letter, Comment | null | IM | 22705457, S0303-8467(12)00280-6, 10.1016/j.clineuro.2012.05.020 | null | Cerebral Infarction, Cerebrovascular Circulation, Female, Humans, Male, Nervous System Diseases | null |
22,705,458 | 2013-07-22 | 2013-02-11 | 1872-6968 | Clinical neurology and neurosurgery | Comparing the cost-effectiveness of two brain metastasis treatment modalities from a payer's perspective: stereotactic radiosurgery versus surgical resection. | Vuong Duong Anh, Rades Dirk, van Eck Albertus T C, Horstmann Gerhard A, Busse Reinhard | eng | null | Journal Article | null | IM | 22705458, S0303-8467(12)00265-X, 10.1016/j.clineuro.2012.05.005 | This study aims to identify the cost-effectiveness of two brain metastatic treatment modalities, stereotactic radiosurgery (SRS) versus surgical resection (SR), from the perspective of Germany's Statutory Health Insurance (SHI) System. | Aged, Algorithms, Anesthesia, General, Brain Neoplasms, Cohort Studies, Cost-Benefit Analysis, Costs and Cost Analysis, Databases, Factual, Female, Germany, Humans, Kaplan-Meier Estimate, Life Expectancy, Magnetic Resonance Imaging, Male, Middle Aged, National Health Programs, Neurosurgical Procedures, Prospective Studies, Radiosurgery, Retrospective Studies, Survival Analysis, Treatment Outcome | null |
22,705,454 | 2013-03-29 | 2022-04-10 | 1873-3379 | Research in developmental disabilities | Cost comparison of early intensive behavioral intervention and treatment as usual for children with autism spectrum disorder in The Netherlands. | Peters-Scheffer Nienke, Didden Robert, Korzilius Hubert, Matson Johnny | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705454, S0891-4222(12)00098-4, 10.1016/j.ridd.2012.04.006 | Early intensive behavioral intervention (EIBI) may result in improved cognitive, adaptive and social functioning and reductions in autism severity and behavioral problems in children with Autism Spectrum Disorder (ASD). For a subset of children, normal functioning may be the result. However, due to the intensity (20-40 h per week for 3 years with a low child staff ratio) implementation costs are high and can be controversial. Estimated costs for education, (supported) work and (sheltered) living for individuals with ASD in The Netherlands are applied in a cost-offset model. A compelling argument for the provision of EIBI is long term savings which are approximately € 1,103,067 from age 3 to 65 years per individual with ASD. Extending these costs to the whole Dutch ASD population, cost savings of € 109.2-€ 182 billion have been estimated, excluding costs associated with inflation. | Adolescent, Adult, Aged, Behavior Therapy, Child, Child Behavior Disorders, Child Development Disorders, Pervasive, Child, Preschool, Cost-Benefit Analysis, Early Intervention, Educational, Education, Special, Female, Humans, Longitudinal Studies, Male, Middle Aged, Netherlands, Outcome and Process Assessment, Health Care, Young Adult | null |
22,705,459 | 2013-01-02 | 2021-10-21 | 1873-4995 | Journal of controlled release : official journal of the Controlled Release Society | Delivery of two-step transcription amplification exendin-4 plasmid system with arginine-grafted bioreducible polymer in type 2 diabetes animal model. | Kim Pyung-Hwan, Lee Minhyung, Kim Sung Wan | eng | R01 DK077703 (NIDDK NIH HHS, United States); DK077703 (NIDDK NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Blood Glucose, Drug Carriers, Hypoglycemic Agents, Insulin, Peptides, Polymers, Venoms, Glucagon-Like Peptide 1, DNA, Arginine, Exenatide | IM | 22705459, S0168-3659(12)00503-2, 10.1016/j.jconrel.2012.06.010, PMC3426869, NIHMS386953, 19962189, 18491986, 17319471, 18820816, 14700743, 16039908, 17369525, 21924767, 21692471, 12409292, 21945681, 21977450, 19007981, 11319721, 20715855, 10837605, 18375745, 21126761, 17803225, 17098089, 21515323, 20305008, 19545590, 11889200, 19558363, 10830274, 14514604, 18984744, 21824270 | Exendin-4, glucagon-like peptide 1 (GLP-1) receptor agonist, is an exocrine hormone, which has potent insulinotropic actions similar to GLP-1 such as stimulating insulin biosynthesis, facilitating glucose concentration dependent insulin secretion, slowing gastric emptying, reducing food intake and stimulating β-cell proliferation. Exendin-4, also, has a longer half-life than GLP-1, due to its resistance to degradation by dipeptidyl peptidase-IV (DPP-IV). In spite of its many advantages as a therapeutic agent for diabetes, its clinical application is still restricted. Thus, to improve the activity of exendin-4 in vivo, gene therapy system was developed as an alternative method. An exendin-4 expression system was constructed using the two-step transcription amplification (TSTA) system, which is composed of pβ-Gal4-p65 and pUAS-SP-exendin-4 with combining the advantages of signal peptide (SP) in order to facilitate its secretion in ectopic cells or tissue. Arginine-grafted cyctaminebisacrylamide-diaminohexane polymer (ABP) was used as a gene carrier. Increased expression of exendin-4, glucose dependent insulin secretion in NIT-1 insulinoma cells, and high insulin expression in the presence of DPP-IV were evaluated in vitro after delivery of ABP/TSTA-SP-exendin-4. Blood glucose levels in diabetic mice were decreased dramatically from the third day for experimental period after single intravenous administration with ABP/TSTA-SP-exendin-4. The highest insulinotropic effect of exendin-4 was also observed in the ABP/TSTA/SP-exendin-4-treated mice groups, compared with the others groups from the 3rd day after injection. TSTA exendin-4 expression system with SP and ABP polymer has a potential gene therapy for the treatment of type 2 diabetes. | Animals, Arginine, Blood Glucose, Cell Line, DNA, Diabetes Mellitus, Type 2, Drug Carriers, Exenatide, Gene Expression, Genetic Therapy, Glucagon-Like Peptide 1, Humans, Hypoglycemic Agents, Insulin, Insulin-Secreting Cells, Male, Mice, Mice, Inbred C57BL, Peptides, Plasmids, Polymers, Transcription, Genetic, Venoms | null |
22,705,455 | 2013-03-29 | 2020-12-09 | 1873-3379 | Research in developmental disabilities | Perceived social acceptance, theory of mind and social adjustment in children with intellectual disabilities. | Fiasse Catherine, Nader-Grosbois Nathalie | eng | null | Journal Article | null | IM | 22705455, S0891-4222(12)00133-3, 10.1016/j.ridd.2012.05.017 | Perceived social acceptance, theory of mind (ToM) and social adjustment were investigated in 45 children with intellectual disabilities (ID) compared with 45 typically developing (TD) preschoolers, matched for developmental age assessed by means of the Differential Scales of Intellectual Efficiency-Revised edition (EDEI-R, Perron-Borelli, 1996). Children's understanding of beliefs and emotions was assessed by means of ToM belief tasks (Nader-Grosbois & Thirion-Marissiaux, 2011) and ToM emotion tasks (Nader-Grosbois & Thirion-Marissiaux, 2011). Seven items from the Pictorial Scale of Perceived Competence and Social Acceptance for children (PSPCSA, Harter & Pike, 1980) assessed children's perceived social acceptance. Their teachers completed the Social Adjustment for Children Scale (EASE, Hughes, Soares-Boucaud, Hochmann, & Frith, 1997). For both groups together, the results showed that perceived social acceptance mediates the relation between ToM skills and social adjustment. The presence or absence of intellectual disabilities does not moderate the relations either between ToM skills and perceived social acceptance, or between perceived social acceptance and social adjustment. The study did not confirm the difference hypothesis of structural and relational patterns between these three processes in children with ID, but instead supported the hypothesis of a similar structure that develops in a delayed manner. | Adolescent, Belgium, Child, Culture, Education, Special, Emotions, Facial Expression, Female, Humans, Intellectual Disability, Interpersonal Relations, Language Development Disorders, Male, Pattern Recognition, Visual, Psychological Distance, Reference Values, Social Adjustment, Social Perception, Theory of Mind | null |
22,705,460 | 2013-01-02 | 2021-10-21 | 1873-4995 | Journal of controlled release : official journal of the Controlled Release Society | The effect of particle size on the biodistribution of low-modulus hydrogel PRINT particles. | Merkel Timothy J, Chen Kai, Jones Stephen W, Pandya Ashish A, Tian Shaomin, Napier Mary E, Zamboni William E, DeSimone Joseph M | eng | R21 HL092814 (NHLBI NIH HHS, United States); P30 CA016086 (NCI NIH HHS, United States); 1R21HL096011 (NHLBI NIH HHS, United States); DP1 CA174425 (NCI NIH HHS, United States); U54 CA151652 (NCI NIH HHS, United States); 1R21HL092814-01 (NHLBI NIH HHS, United States); R21 HL096011 (NHLBI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Acrylates, Drug Carriers, polyethylene glycol-dihydroxyl, Hydrogel, Polyethylene Glycol Dimethacrylate, Polyethylene Glycols | IM | 22705460, S0168-3659(12)00502-0, 10.1016/j.jconrel.2012.06.009, PMC3416965, NIHMS392221, 18182283, 19025903, 18758474, 16443279, 21590884, 16876898, 18373181, 17643544, 10179196, 11339981, 18697944, 18679800, 18672949, 21809808, 18840488, 15207459, 19538994, 21220299, 10176140, 20945925, 19096389, 20000620, 16011375, 20018694, 12219820, 19562807, 20183836 | There is a growing recognition that the deformability of particles used for drug delivery plays a significant role on their biodistribution and circulation profile. Understanding these effects would provide a crucial tool for the rational design of drug delivery systems. While particles resembling red blood cells (RBCs) in size, shape and deformability have extended circulation times and altered biodistribution profiles compared to rigid, but otherwise similar particles, the in vivo behavior of such highly deformable particles of varied size has not been explored. We report the fabrication of a series of discoid, monodisperse, low-modulus hydrogel particles with diameters ranging from 0.8 to 8.9 μm, spanning sizes smaller than and larger than RBCs. We injected these particles into healthy mice, and tracked their concentration in the blood and their distribution into major organs. These deformable particles all demonstrated some hold up in filtration tissues like the lungs and spleen, followed by release back into the circulation, characterized by decreases in particles in these tissues with concomitant increases in particle concentration in blood. Particles similar to red blood cells in size demonstrated longer circulation times, suggesting that this size and shape of deformable particle is uniquely suited to avoid clearance. | Acrylates, Animals, Biomimetic Materials, Drug Carriers, Elastic Modulus, Erythrocytes, Female, Hydrogel, Polyethylene Glycol Dimethacrylate, Mice, Mice, Inbred BALB C, Particle Size, Polyethylene Glycols, Tissue Distribution | null |
22,705,462 | 2012-12-26 | 2020-12-09 | 1567-7257 | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Comparative analysis of an expanded Clostridium difficile reference strain collection reveals genetic diversity and evolution through six lineages. | Knetsch Cornelis W, Terveer Elisabeth M, Lauber Chris, Gorbalenya Alexander E, Harmanus Céline, Kuijper Ed J, Corver Jeroen, van Leeuwen Hans C | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705462, S1567-1348(12)00218-3, 10.1016/j.meegid.2012.06.003 | Clostridium difficile is an anaerobic bacillus that resides in the gut and has rapidly emerged as a leading cause of antibiotic associated diarrheal disease in humans. The genetic basis of the pathogenicity of C. difficile remains poorly understood. In this study we aimed at characterizing the genetic diversity of C. difficile strains by three different methods (PCR ribotyping, multilocus sequence typing and genetic markers) to improve the typing of C. difficile. Our study was performed on a reference collection (Leeds-Leiden/ECDC) of C. difficile PCR ribotype (RT) strains (n=70) expanded with six PCR RT strains highly related to the emerging PCR RTs 027 and 078. Besides PCR ribotyping we used multilocus sequence typing (MLST) using seven housekeeping genes (MLST 7HG) that has recently been developed for characterizing C. difficile isolates as well as analysis of unique genetic markers. Evolutionary relatedness of the sequences determined by MLST 7HG was analyzed in phylogenetic analysis. In total 56 MLST 7HG sequence types (STs) were identified, nine of which were new. Phylogeny reconstruction of the reference set of strains supplemented with the online available C. difficile MLST reference database, revealed six monophyletic lineages of closely related STs. ST-122 (PCR RT131) formed a well-separated branch in the tree and was thus designated as a novel lineage. Furthermore, we confirmed that several PCR RTs are highly related to the emerging PCR RTs 027 and 078 since these types display the same STs (ST-1 and ST-11, respectively). Based on the observed results, we conclude that MLST 7HG is a valuable method to study C. difficile phylogeny. | Bayes Theorem, Clostridioides difficile, Evolution, Molecular, Genes, Bacterial, Genes, Essential, Genetic Variation, Likelihood Functions, Models, Genetic, Multilocus Sequence Typing, Phylogeny, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Reference Standards | null |
22,705,461 | 2013-01-02 | 2021-10-21 | 1873-4995 | Journal of controlled release : official journal of the Controlled Release Society | Polymer nanoparticles encapsulating siRNA for treatment of HSV-2 genital infection. | Steinbach Jill M, Weller Caroline E, Booth Carmen J, Saltzman W Mark | eng | F32 AI093056 (NIAID NIH HHS, United States); T32 HG003198 (NHGRI NIH HHS, United States); R01 EB000487 (NIBIB NIH HHS, United States); T32-HG003198 (NHGRI NIH HHS, United States); F32AI093056-01A1 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Cell Adhesion Molecules, Drug Carriers, Nectins, RNA, Small Interfering, Polylactic Acid-Polyglycolic Acid Copolymer, Polyglycolic Acid, Lactic Acid | IM | 22705461, S0168-3659(12)00501-9, 10.1016/j.jconrel.2012.06.008, PMC3543782, NIHMS386952, 18026169, 16320000, 16766229, 24470908, 21315117, 16926356, 19379812, 21356167, 16741997, 21576818, 14602572, 6296440, 12663762, 16639430, 20017659, 18949218, 19532067, 16282463, 19627185, 21109069, 16306938, 19194802, 15021308, 19874851, 22064530, 19154990, 14963155, 18186706, 163794, 20643915, 18992405, 17094789, 19404239 | Effective, low-cost, and safe treatments for sexually transmitted viral infections are urgently needed. Here, we show for the first time that intravaginal administration with nanoparticles of poly(lactic-co-glycolic acid) (PLGA) encapsulating short interfering RNA (siRNA) molecules is effective for prevention of genital HSV-2 infections in mice. PLGA nanoparticles (NPs) were designed to interfere with HSV-2 infection by siRNA-mediated knockdown of nectin, a host cell protein. NPs were characterized in vitro to determine the optimal formulation based on siRNA loading, controlled release profile, and mRNA knockdown. Mice inoculated intravaginally with a lethal dose of HSV-2, and treated with PLGA NPs, showed increased survival from ~9 days (in untreated mice) to >28 days (in PLGA NP treated mice) - the longest survival ever observed with siRNA treatment in this mouse model. This work provides proof-of-concept that topical administration of NPs containing siRNA against a pathologically relevant host cell target can knockdown the gene in tissue and improve survival after HSV-2 infection. Furthermore, this system provides a safe delivery platform that employs materials that are already approved by the FDA and can be modified to enhance delivery of other microbicides. | Animals, Cell Adhesion Molecules, Drug Carriers, Female, Genetic Therapy, HeLa Cells, Herpes Genitalis, Herpesvirus 2, Human, Host-Pathogen Interactions, Humans, Lactic Acid, Mice, Mice, Inbred C57BL, Nanoparticles, Nectins, Polyglycolic Acid, Polylactic Acid-Polyglycolic Acid Copolymer, RNA Interference, RNA, Small Interfering | null |
22,705,463 | 2012-12-26 | 2021-10-21 | 1567-7257 | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Translational selection of genes coding for perfectly conserved proteins among three mosquito vectors. | Rodriguez Olaf, Singh Brajendra K, Severson David W, Behura Susanta K | eng | R01 AI059342 (NIAID NIH HHS, United States); R01 AI079125 (NIAID NIH HHS, United States); R01-AI059342 (NIAID NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | Codon, Insect Proteins | IM | 22705463, S1567-1348(12)00220-1, 10.1016/j.meegid.2012.06.005, PMC3428495, NIHMS390403, 9461401, 18842120, 16973573, 17537810, 21942845, 17488738, 17786218, 9628917, 15258291, 9223264, 17686799, 18039870, 17510324, 18983258, 20929810, 20047958, 11139289, 22039174, 1752426, 21040044, 14668244, 3447015, 20167017, 12045147, 7849098, 10553904, 12364791, 22579482, 18583614, 15271077, 15479947, 18192698, 7559409, 15371247, 10234248, 14990797, 21102527, 18005411, 15222899, 15008418 | The biased usage of synonymous codons affects translational efficiency of genes. We studied codon usage patterns of genes that are perfectly conserved at the amino acid level among three important mosquito vector species: Aedes aegypti (vector of dengue virus), Anopheles gambiae (vector of malaria) and Culex quinquefasciatus (vector of lymphatic filariasis and West Nile Virus). Although these proteins have the same amino acid sequences, non-random usage of synonymous codons is evident among the orthologous genes. The coding sequences of these genes were simulated to generate random mutation sites to be further investigated for patterns of codon bias. It was found that codon usage bias is significantly higher in genes that represented perfectly conserved proteins than genes where variation was apparent at the amino acid sequence. Our results suggest that genes coding for perfectly conserved proteins are highly biased with optimized codons and may be under stringent translational selection in these vector species. | Aedes, Amino Acid Sequence, Animals, Anopheles, Base Composition, Cluster Analysis, Codon, Conserved Sequence, Culex, Evolution, Molecular, Insect Proteins, Insect Vectors, Logistic Models, Models, Genetic, Molecular Sequence Data, Mutation, Phylogeny, Protein Biosynthesis | null |
22,705,467 | 2013-02-04 | 2012-11-26 | 1532-821X | Archives of physical medicine and rehabilitation | Specificity of the femoral slump test for the assessment of experimentally induced anterior knee pain. | Lai Weng-Hang, Shih Yi-Fen, Lin Pei-Ling, Chen Wen-Yin, Ma Hsiao-Li | eng | null | Journal Article | null | IM | 22705467, S0003-9993(12)00408-X, 10.1016/j.apmr.2012.06.003 | To assess the specificity of the femoral slump test (FST) when assessing experimentally induced anterior knee pain. | Adult, Cross-Sectional Studies, Female, Humans, Knee, Male, Pain, Physical Therapy Modalities, Range of Motion, Articular, Reproducibility of Results | null |
22,705,464 | 2012-10-18 | 2012-08-13 | 1872-8421 | Journal of neuroimmunology | Clinical and magnetic resonance imaging features of multiple sclerosis with autoreactive antibodies in Ishikawa Prefecture, Japan. | Takahashi Kazuya, Tanaka Keiko | eng | null | Journal Article | Autoantibodies | IM | 22705464, S0165-5728(12)00171-3, 10.1016/j.jneuroim.2012.05.017 | Previous reports of multiple sclerosis (MS) with autoantibodies might include neuromyelitis optica (NMO). We investigated the frequency of autoreactive antibodies (AR) in both MS and NMO. Systemic lupus erythematosus (SLE)-associated autoantibodies such as anti-Sm antibodies, anti-single stranded DNA antibodies and lupus anticoagulants were only identified in MS, whereas SLE itself is more commonly associated with NMO. Moreover, when magnetic resonance imaging features between autoreactive antibody-positive (AR(+))MS and -negative (AR(-))MS were compared, AR(+)MS cases showed significantly fewer than 3 periventricular lesions compared to AR(-)MS cases. These results may indicate different pathogenetic mechanisms underlying AR(+)MS and AR(-)MS. | Adolescent, Adult, Autoantibodies, Central Nervous System, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Female, Humans, Japan, Magnetic Resonance Imaging, Male, Middle Aged, Multiple Sclerosis, Neuromyelitis Optica, Young Adult | null |
22,705,466 | 2013-02-04 | 2022-03-11 | 1532-821X | Archives of physical medicine and rehabilitation | Evaluation of a new motion sensor in patients with chronic obstructive pulmonary disease. | Sant'Anna Thaís, Escobar Victoria C, Fontana Andréa D, Camillo Carlos A, Hernandes Nidia A, Pitta Fabio | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705466, S0003-9993(12)00407-8, 10.1016/j.apmr.2012.05.027 | To assess the criterion validity and reproducibility of a new pedometer in patients with chronic obstructive pulmonary disease (COPD). | Activities of Daily Living, Aged, Cross-Sectional Studies, Exercise, Female, Humans, Male, Middle Aged, Monitoring, Ambulatory, Physical Therapy Modalities, Pulmonary Disease, Chronic Obstructive, Reproducibility of Results, Walking | null |
22,705,465 | 2013-02-04 | 2013-11-21 | 1532-821X | Archives of physical medicine and rehabilitation | Influence of contraction type, speed, and joint angle on ankle muscle weakness in Parkinson's disease: implications for rehabilitation. | Pang Marco Y, Mak Margaret K | eng | null | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705465, S0003-9993(12)00409-1, 10.1016/j.apmr.2012.06.004 | To compare the ankle muscle strength and torque-angle relationship between individuals with Parkinson's disease (PD) and participants without impairments. | Aged, Ankle, Biomechanical Phenomena, Cross-Sectional Studies, Humans, Middle Aged, Muscle Contraction, Muscle Strength, Muscle Weakness, Parkinson Disease, Range of Motion, Articular, Torque | null |
22,705,468 | 2013-02-04 | 2012-11-26 | 1532-821X | Archives of physical medicine and rehabilitation | K-means cluster analysis of rehabilitation service users in the Home Health Care System of Ontario: examining the heterogeneity of a complex geriatric population. | Armstrong Joshua J, Zhu Mu, Hirdes John P, Stolee Paul | eng | ETG-92249 (Canadian Institutes of Health Research, Canada) | Journal Article, Research Support, Non-U.S. Gov't | null | IM | 22705468, S0003-9993(12)00406-6, 10.1016/j.apmr.2012.05.026 | To examine the heterogeneity of home care clients who use rehabilitation services by using the K-means algorithm to identify previously unknown patterns of clinical characteristics. | Aged, Aged, 80 and over, Algorithms, Cluster Analysis, Female, Geriatric Assessment, Health Services for the Aged, Home Care Services, Humans, Male, Middle Aged, Ontario, Physical Therapy Specialty | null |
22,705,470 | 2012-10-26 | 2012-11-15 | 1361-6528 | Nanotechnology | Evaluation of all-inorganic CdSe quantum dot thin films for optoelectronic applications. | Zhang Y Q, Cao X A | eng | null | Journal Article | Cadmium Compounds, Inorganic Chemicals, Membranes, Artificial, Selenium Compounds, cadmium selenide | IM | 22705470, 10.1088/0957-4484/23/27/275702 | Exchanging the original organic ligands of colloidal CdSe quantum dots (QDs) with inorganic metal chalcogenide SnS(4) ligands resulted in absorption peak redshifts and complete photoluminescence (PL) quenching in QD solids. The SnS(4)-capped QDs, meanwhile, were able to retain strong excitonic absorption. After the ligand exchange, the ITO/QDs/Al structure showed much higher electrical conductivity and reduced space-charge limited current. Its photocurrent spectral response increased by over two orders of magnitude and closely resembled the absorption spectrum of the QDs. However, it was found that mild thermal treatment above 200 °C transformed the SnS(4)-capped QD film into to a more conductive assembly, degrading its absorption and photocurrent generation. These results suggest that the inorganic ligands considerably enhanced the inter-dot electronic coupling in QD solids, leading to facile charge separation and transport. Our study thus demonstrates the potential applicability of colloidal QDs with metal chalcogenide ligands processed at low temperatures for efficient photodetection and solar energy conversion. | Cadmium Compounds, Conductometry, Inorganic Chemicals, Materials Testing, Membranes, Artificial, Photometry, Quantum Dots, Selenium Compounds | null |
22,705,469 | 2012-11-19 | 2018-12-01 | 1873-2399 | Experimental hematology | Impact of lenalidomide on the functional properties of human mesenchymal stromal cells. | Wobus Manja, Benath Gwendolin, Ferrer Ruben A, Wehner Rebekka, Schmitz Marc, Hofbauer Lorenz C, Rauner Martina, Ehninger Gerhard, Bornhäuser Martin, Platzbecker Uwe | eng | null | Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't | Antineoplastic Agents, CXCL12 protein, human, Chemokine CXCL12, GPI-Linked Proteins, Integrin beta1, Thalidomide, 5'-Nucleotidase, NT5E protein, human, Lenalidomide | IM | 22705469, S0301-472X(12)00230-5, 10.1016/j.exphem.2012.06.004 | Lenalidomide (LEN) has emerged as a promising therapeutic option for the management of various hematologic malignancies. Although its direct mechanisms of action on malignant cells have been studied intensively, its effects on the stromal compartment of bone marrow have not yet been analyzed systematically. Therefore, we investigated whether LEN alters the functional capacity of mesenchymal stromal cells (MSCs) as the main cellular component of the bone marrow microenvironment. In addition to their growth and differentiation characteristics, we focused on the ability of MSC to modulate T-cell function and support hematopoietic stem cells (HSCs). | 5'-Nucleotidase, Adipogenesis, Adult, Antineoplastic Agents, Bone Marrow Cells, Cell Differentiation, Chemokine CXCL12, Female, GPI-Linked Proteins, Hematologic Neoplasms, Humans, Integrin beta1, Lenalidomide, Male, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells, Middle Aged, Osteogenesis, Thalidomide, Transplantation, Homologous | null |
22,705,471 | 2013-01-07 | 2013-11-21 | 1873-507X | Physiology & behavior | Swimming exercise during pregnancy alleviates pregnancy-associated long-term memory impairment. | Kim Kijeong, Chung Eunhee, Kim Chang-Ju, Lee Sukho | eng | null | Journal Article | Bromodeoxyuridine | IM | 22705471, S0031-9384(12)00228-4, 10.1016/j.physbeh.2012.06.004 | Regular exercise has been shown to be beneficial to the brain functions, but little is known about the effects of exercise during pregnancy on the long-term memory function of the mothers. The objective of this study was to determine the effects of swimming during pregnancy on long-term memory function in rats on postpartum day 8. We examined the impact of swimming exercise during pregnancy on cell proliferation and apoptotic neuronal cell death in the hippocampus of peripartum rats. The rats were divided into three groups: the control group, the pregnant non-swimming group, and the pregnant swimming group. We found that pregnancy impaired the long-term memory while swimming during pregnancy alleviated the memory impairment. Pregnancy decreased cell proliferation in the dentate gyrus of the hippocampus, but swimming exercise during pregnancy reversed pregnancy-associated decreased cell proliferation back to control level. There was no difference in apoptotic neuronal cell death in the hippocampus among groups. Our results suggest that swimming during pregnancy alleviates pregnancy-associated decrease in memory function of mothers through an increase in cell proliferation in the hippocampus. | Analysis of Variance, Animals, Avoidance Learning, Body Weight, Bromodeoxyuridine, Cell Death, Cell Proliferation, Exploratory Behavior, Female, Hippocampus, In Situ Nick-End Labeling, Male, Maternal Behavior, Memory Disorders, Memory, Long-Term, Neurons, Physical Conditioning, Animal, Pregnancy, Pregnancy Complications, Rats, Rats, Sprague-Dawley, Reaction Time, Swimming | null |
22,705,473 | 2012-10-31 | 2012-07-09 | 1879-0003 | International journal of biological macromolecules | Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions. | Cho Hee Jung, Ki Chang Seok, Oh Hanjin, Lee Ki Hoon, Um In Chul | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Silk, Solutions, Fibroins | IM | 22705473, S0141-8130(12)00223-1, 10.1016/j.ijbiomac.2012.06.007 | Four regenerated silk fibroin (SF) samples were prepared under different dissolution conditions and their molecular weight (MW) distributions and solution properties in water and formic acid were examined. SFL, produced by dissolving in LiBr aqueous solution for 6h, showed the highest MW level. In the three SFC samples, produced by dissolving SF in CaCl(2)/H(2)O/EtOH solution for dissolution times ranging from 3 to 180 min, the MW of the SFs decreased with increasing dissolution time and a new band appeared at low MW. Interestingly, SFL presented as a relatively transparent aqueous solution with 10-30 nm particle size, whereas the three SFC samples exhibited a turbid solution with 100-300 nm particle size. SF formic acid solutions showed a higher viscosity than SF aqueous solutions and exhibited almost Newtonian fluid behavior, whereas SF aqueous solutions displayed abrupt shear thinning in the low shear rate region (0.1-3 s(-1)). | Animals, Bombyx, Fibroins, Molecular Weight, Nephelometry and Turbidimetry, Particle Size, Rheology, Silk, Solutions | null |
22,705,472 | 2013-01-14 | 2016-11-25 | 1879-0003 | International journal of biological macromolecules | Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide. | Wei Dongfeng, Wei Yanxia, Cheng Weidong, Zhang Lifeng | eng | null | Journal Article | Antineoplastic Agents, Imidazoles, Polysaccharides, Solvents, Sulfates, 1-butyl-3-methylimidazolium chloride, 4-Aminopyridine, 4-dimethylaminopyridine | IM | 22705472, S0141-8130(12)00220-6, 10.1016/j.ijbiomac.2012.06.004 | Sulfated modification of a polysaccharide obtained from Radix hedysari (RHP) was studied. Four sulfated derivatives (RHPS) with variable degrees of substitution (DS) were obtained by the chlorosulfonic acid method with ionic liquids (ILs) as solvent and 4-dimethylaminopyridine (DMAP) as catalyst. The structures of RHPS were characterized by FT-IR spectra and ¹³C NMR spectra, and the results indicated that the sulfated groups were modified mainly at the C-6 position and C-2 position. Four kinds of RHPS showed different DS ranging from 0.63 to 1.45, and different weight-average molecular mass (Mw) ranging from 60.8 to 71.1 kDa with a little degradation. Compared with RHP, all of RHPS exhibited obvious antitumor activity on A549 cells and BGC-823 cells in vitro. However, they had no obvious influence on HEK293 cells, which indicated that they had low toxicity to normal cells. Flow cytometric studies indicated that the treatment of RHPS against A549 cells and BGC-823 cells could mediate the cell-cycle arrest in the G1 phase. | 4-Aminopyridine, Antineoplastic Agents, Apoptosis, Catalysis, Cell Cycle Checkpoints, Cell Line, Tumor, Cell Proliferation, Fabaceae, HEK293 Cells, Humans, Imidazoles, Kinetics, Molecular Weight, Plant Roots, Polymerization, Polysaccharides, Solvents, Structure-Activity Relationship, Sulfates | null |
22,705,474 | 2013-01-14 | 2012-09-03 | 1879-0003 | International journal of biological macromolecules | Effect of "in vitro" induced glycation on thermostability of bone tissue. | Trębacz Hanna, Wójtowicz Krzysztof, Wlizło-Dyś Ewa, Dyś Wojciech | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Collagen | IM | 22705474, S0141-8130(12)00219-X, 10.1016/j.ijbiomac.2012.06.003 | The aim of the study was to test the hypothesis that glycation would influence thermal stability of bone tissue collagen. Bone samples were incubated in buffer or in ribose solution. Then, half of the ribosylated and half of the control samples were completely demineralized in formic acid. Differential scanning calorimetry was performed for temperatures from 40 °C to 220 °C in nitrogen atmosphere on intact (mineralized) and demineralized bone samples, partially dehydrated at room temperature. Samples were thermally active in temperatures from 110 °C to 210 °C. Few endotherms of a complex nature were found in demineralized and intact bone. Thermodynamics of collagen conformations was affected by glycation, especially in demineralized bone where a significant increase of denaturation temperature (by 3-4 °C) and enthalpy drop (above 20%) were stated after glycation. | Animals, Calcification, Physiologic, Cattle, Collagen, Femur, Glycosylation, Protein Stability, Temperature | null |
22,705,475 | 2013-01-14 | 2017-11-16 | 1879-0003 | International journal of biological macromolecules | Structural insights of rohu TLR3, its binding site analysis with fish reovirus dsRNA, poly I:C and zebrafish TRIF. | Sahoo Bikash R, Basu Madhubanti, Swain Banikalyan, Maharana Jitendra, Dikhit Manas R, Jayasankar Pallipuram, Samanta Mrinal | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Adaptor Proteins, Vesicular Transport, Fish Proteins, Glycoproteins, RNA, Double-Stranded, RNA, Viral, TICAM1 protein, zebrafish, Toll-Like Receptor 3, Zebrafish Proteins, Poly I-C | IM | 22705475, S0141-8130(12)00221-8, 10.1016/j.ijbiomac.2012.06.005 | In response to double stranded RNA (dsRNA) viruses, toll-like receptor 3 (TLR3) in fish activates signaling like human, and induces innate immunity. This suggested the existence of dsRNA binding domains in fish TLR3 as reported in higher vertebrates. In in silico analysis, leucine rich repeat (LRR) regions (4-6, 13-14, 20-22), and LRR (8-15, 17-24) were identified as key domains in rohu TLR3 as poly I:C and dsRNA of fish reovirus (AGCRV,VHSV and IHNV) binding regions. 3D-models of rohu TLR3-TIR and zebrafish TRIF were generated by homology and ab initio modeling respectively, and their interacting domains were predicted. This is the first report of TLR3 modeling in fish. | Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Animals, Binding Sites, Cyprinidae, Fish Proteins, Glycoproteins, Humans, Molecular Dynamics Simulation, Molecular Sequence Data, Mutagenesis, Site-Directed, Poly I-C, Protein Structure, Tertiary, RNA, Double-Stranded, RNA, Viral, Reoviridae, Toll-Like Receptor 3, Zebrafish, Zebrafish Proteins | null |
22,705,477 | 2012-10-29 | 2012-07-23 | 1095-564X | Developmental biology | Extracellular matrix assembly and 3D organization during paraxial mesoderm development in the chick embryo. | Rifes Pedro, Thorsteinsdóttir Sólveig | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Fibronectins, Laminin | IM | 22705477, S0012-1606(12)00312-0, 10.1016/j.ydbio.2012.06.003 | The extracellular matrix (ECM) is a major player in the microenvironment governing morphogenesis. However, much is yet to be known about how matrix composition and architecture changes as it influences major morphogenetic events. Here we performed a detailed, 3D analysis of the distribution of two ECM components, fibronectin and laminin, during the development of the chick paraxial mesoderm. By resorting to whole mount double immunofluorescence and confocal microscopy, we generated a detailed 3D map of the two ECM components, revealing their supra-cellular architecture in vivo, while simultaneously retaining high resolution cellular detail. We show that fibronectin assembly occurs at the surface of the presomitic mesoderm (PSM), where a gradual increase in the complexity of the fibronectin matrix accompanies PSM maturation. In the rostral PSM, where somites form, fibronectin fibrils are thick and densely packed and some occupy the cleft which comes to separate the newly formed somite from the PSM. Our 3D approach revealed that laminin matrix assembly starts at the PSM surface as small dispersed patches, which are always localized closer to cells than the fibronectin matrix. These patches gradually grow and coalesce with neighboring patches, but do not generate a continuous laminin sheet, not even on epithelial somites and dermomyotome, suggesting that these epithelia develop in contact with a fenestrated laminin matrix. Unexpectedly, as the somite differentiates, its fibronectin and laminin matrices are maintained, thus initially containing both the epithelial dermomyotome and the mesenchymal sclerotome within the somite segment. Our analysis provides unprecedented details of the progressive in vivo assembly and 3D architecture of fibronectin and laminin matrices during paraxial mesoderm development. These data are consistent with the hypothesis that progressive ECM assembly and subsequent 3D organization are active driving and containing forces during tissue development. | Animals, Body Patterning, Chick Embryo, Extracellular Matrix, Fibronectins, Fluorescent Antibody Technique, Imaging, Three-Dimensional, Laminin, Mesoderm, Microscopy, Confocal, Models, Anatomic, Somites | null |
22,705,476 | 2012-12-13 | 2024-02-08 | 1878-0180 | Journal of the mechanical behavior of biomedical materials | Comparing coronary stent material performance on a common geometric platform through simulated bench testing. | Grogan J A, Leen S B, McHugh P E | eng | null | Comparative Study, Journal Article, Research Support, Non-U.S. Gov't | Alloys, Biocompatible Materials, Chromium Alloys, Metals, Stainless Steel, Iron, Magnesium | IM | 22705476, S1751-6161(12)00064-1, 10.1016/j.jmbbm.2012.02.013 | Absorbable metallic stents (AMSs) are a newly emerging cardiovascular technology which has the potential to eliminate long-term patient health risks associated with conventional permanent stents. AMSs developed to date have consisted of magnesium alloys or iron, materials with inferior mechanical properties to those used in permanent stents, such as stainless steel and cobalt-chromium alloys. However, for AMSs to be feasible for widespread clinical use it is important that their performance is comparable to modern permanent stents. To date, the performances of magnesium, iron, and permanent stent materials have not been compared on a common stent platform for a range of stent performance metrics, such as flexibility, radial strength, and recoil. In this study, this comparison is made through simulated bench testing, based on finite-element modelling. The significance of this study is that it allows potential limitations in current AMS performance to be identified, which will aid in focusing future AMS design. This study also allows the identification of limitations in current AMS materials, thereby informing the on-going development of candidate biodegradable alloys. The results indicate that the AMSs studied here can match the recoil characteristics and radial strength of modern permanent stents; however, to achieve this, larger strut dimensions are required. It is also predicted that the AMSs studied are inferior to permanent stents in terms of maximum absolute curvature and longitudinal stiffness. | Absorbable Implants, Absorption, Alloys, Angioplasty, Balloon, Coronary, Biocompatible Materials, Biomechanical Phenomena, Chromium Alloys, Computer Simulation, Coronary Vessels, Finite Element Analysis, Humans, Iron, Magnesium, Materials Testing, Metals, Prosthesis Design, Prosthesis Failure, Stainless Steel, Stents, Stress, Mechanical, Tensile Strength | null |
22,705,478 | 2012-10-29 | 2021-10-21 | 1095-564X | Developmental biology | A cell-autonomous defect in skeletal muscle satellite cells expressing low levels of survival of motor neuron protein. | Hayhurst Monica, Wagner Amanda K, Cerletti Massimiliano, Wagers Amy J, Rubin Lee L | eng | P01 NS066888 (NINDS NIH HHS, United States); P30 DK036836 (NIDDK NIH HHS, United States); P01NS066888-01A1 (NINDS NIH HHS, United States); P30DK036836 (NIDDK NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't | PAX7 Transcription Factor, Pax7 protein, mouse, Smn1 protein, mouse, Survival of Motor Neuron 1 Protein, Survival of Motor Neuron 2 Protein, Syndecans | IM | 22705478, S0012-1606(12)00307-7, 10.1016/j.ydbio.2012.05.037, PMC3851302, NIHMS493767, 9110173, 12743106, 7813012, 11030621, 10655541, 18791638, 12023986, 11791208, 18178576, 11457764, 14645852, 10339583, 11238465, 9259265, 18768931, 20081841, 18065780, 14623865, 18521935, 11994964, 16141372, 16219305, 21173238, 18492800, 7741893, 11302686, 20081842, 18614009, 20425235, 16051152, 3614658, 11784020, 16143100, 7532173, 15537543, 21658376, 17353360, 9245983, 18603534, 12952942, 9806538, 9173917, 17540178, 15703193 | Mutations in the Survival of Motor Neuron (SMN) gene underlie the development of spinal muscular atrophy (SMA), which currently represents the leading genetic cause of mortality in infants and toddlers. SMA is characterized by degeneration of spinal cord motor neurons and muscle atrophy. Although SMA is often considered to be a motor neuron disease, accumulating evidence suggests that muscle cells themselves may be affected by low levels of SMN. Here, we examine satellite cells, tissue-resident stem cells that play an essential role in the growth and repair of skeletal muscle, isolated from a severe SMA mouse model (Smn(-/-); SMN2(+/+)). We found similar numbers of satellite cells in the muscles of SMA and wild-type (Smn(+/+); SMN2(+/+)) mice at postnatal day 2 (P2), and, when isolated from skeletal muscle using cell surface marker expression, these cells showed comparable survival and proliferative potential. However, SMA satellite cells differentiate abnormally, revealed by the premature expression of muscle differentiation markers, and, especially, by a reduced efficiency in forming myotubes. These phenotypes suggest a critical role of SMN protein in the intrinsic regulation of muscle differentiation and suggest that abnormal muscle development contributes to the manifestation of SMA symptoms. | Animals, Animals, Newborn, Apoptosis, Cell Differentiation, Cell Proliferation, Cell Survival, Cells, Cultured, Disease Models, Animal, Flow Cytometry, Humans, Immunohistochemistry, Mice, Mice, Knockout, Mice, Transgenic, Microscopy, Fluorescence, Muscle Fibers, Skeletal, Muscular Atrophy, Spinal, PAX7 Transcription Factor, Satellite Cells, Skeletal Muscle, Survival of Motor Neuron 1 Protein, Survival of Motor Neuron 2 Protein, Syndecans, Time Factors | null |
22,705,480 | 2013-01-11 | 2022-01-29 | 1873-6246 | Biological psychology | Consolidation of temporal order in episodic memories. | Griessenberger H, Hoedlmoser K, Heib D P J, Lechinger J, Klimesch W, Schabus M | eng | P 21154 (Austrian Science Fund FWF, Austria) | Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't | null | IM | 22705480, S0301-0511(12)00132-9, 10.1016/j.biopsycho.2012.05.012, PMC3427018, UKMS49704, 7546307, 10531452, 17324046, 8036517, 23968216, 10476004, 15164890, 17071229, 10442025, 10209231, 6329428, 17440612, 14221692, 12804689, 16623830, 2748771, 15165527, 15838184, 20046194, 7129455, 20977513, 19251443, 15062867, 19245368, 9856467, 21812555, 15802203, 11976705, 16476669, 17086200, 15576885, 11100156, 10650147, 21270783, 21371881, 19376746, 19682856, 12653991, 12486189, 5172928, 16120103, 11100141, 10862947, 15627601, 18714787, 15683137, 16251952, 11691982, 1027738, 15184907, 16911026 | Even though it is known that sleep benefits declarative memory consolidation, the role of sleep in the storage of temporal sequences has rarely been examined. Thus we explored the influence of sleep on temporal order in an episodic memory task followed by sleep or sleep deprivation. Thirty-four healthy subjects (17 men) aged between 19 and 28 years participated in the randomized, counterbalanced, between-subject design. Parameters of interests were NREM/REM cycles, spindle activity and spindle-related EEG power spectra. Participants of both groups (sleep group/sleep deprivation group) performed retrieval in the evening, morning and three days after the learning night. Results revealed that performance in temporal order memory significantly deteriorated over three days only in sleep deprived participants. Furthermore our data showed a positive relationship between the ratios of the (i) first NREM/REM cycle with more REM being associated with delayed temporal order recall. Most interestingly, data additionally indicated that (ii) memory enhancers in the sleep group show more fast spindle related alpha power at frontal electrode sites possibly indicating access to a yet to be consolidated memory trace. We suggest that distinct sleep mechanisms subserve different aspects of episodic memory and are jointly involved in sleep-dependent memory consolidation. | Adult, Brain, Brain Waves, Electroencephalography, Female, Humans, Male, Memory, Episodic, Neuropsychological Tests, Polysomnography, Sleep, Sleep Deprivation | null |
22,705,479 | 2012-11-08 | 2021-10-21 | 1095-564X | Developmental biology | Testis development requires the repression of Wnt4 by Fgf signaling. | Jameson Samantha A, Lin Yi-Tzu, Capel Blanche | eng | R01 HD039963 (NICHD NIH HHS, United States); R37 HD039963 (NICHD NIH HHS, United States); T32 HD040372 (NICHD NIH HHS, United States); 5R01-HD039963-13 (NICHD NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | DNA Primers, Fibroblast Growth Factor 9, Wnt4 Protein, Wnt4 protein, mouse, Fibroblast Growth Factor 2 | IM | 22705479, S0012-1606(12)00318-1, 10.1016/j.ydbio.2012.06.009, PMC3634333, NIHMS402296, 2030730, 19211811, 15567719, 17940049, 18953701, 18927154, 22128028, 18617533, 11290325, 22438826, 8787755, 18155190, 12874059, 18250097, 12949260, 15581876, 12640619, 11283799, 18403409, 17636127, 15229180, 20833365, 7990960, 14736745, 8475082, 18985752, 10412367, 19184501, 15132997, 19884258, 9989404, 16700629, 20153744, 16452457, 15318244, 18829540, 18454134, 20159962, 12835383, 19027189, 8782821, 16099833, 19389346, 2374589, 19403927, 12756187, 8001137, 15385158, 16214126, 16937416, 15211353, 18250098, 15056615, 11431689, 11872831, 11719692, 20040496, 18981061, 11101852, 9486644, 17041600, 10588843, 7600978, 11891111 | The bipotential gonad expresses genes associated with both the male and female pathways. Adoption of the male testicular fate is associated with the repression of many female genes including Wnt4. However, the importance of repression of Wnt4 to the establishment of male development was not previously determined. Deletion of either Fgf9 or Fgfr2 in an XY gonad resulted in up-regulation of Wnt4 and male-to-female sex reversal. We investigated whether the deletion if Wnt4 could rescue sex reversal in Fgf9 and Fgfr2 mutants. XY Fgf9/Wnt4 and Fgfr2/Wnt4 double mutants developed testes with male somatic and germ cells present, suggesting that the primary role of Fgf signaling is the repression of female-promoting genes. Thus, the decision to adopt the male fate is based not only on whether male genes, such as Sox9, are expressed, but also on the active repression of female genes, such as Wnt4. Because loss of Wnt4 results in the up-regulation of Fgf9, we also tested the possibility that derepression of Fgf9 was responsible for the aspects of male development observed in XX Wnt4 mutants. However, we found that the relationship between these two signaling factors is not symmetric: loss of Fgf9 in XX Wnt4(-/-) gonads does not rescue their partial female-to-male sex-reversal. | Animals, DNA Primers, Female, Fibroblast Growth Factor 2, Fibroblast Growth Factor 9, Gene Expression Regulation, Developmental, Male, Mice, Microscopy, Fluorescence, Real-Time Polymerase Chain Reaction, Sex Determination Processes, Signal Transduction, Testis, Wnt4 Protein | null |
22,705,481 | 2012-12-10 | 2021-10-21 | 1873-5843 | Archives of clinical neuropsychology : the official journal of the National Academy of Neuropsychologists | Implications of apathy for everyday functioning outcomes in persons living with HIV infection. | Kamat Rujvi, Woods Steven Paul, Marcotte Thomas D, Ellis Ronald J, Grant Igor | eng | P30 MH062512 (NIMH NIH HHS, United States); P01-DA12065 (NIDA NIH HHS, United States); MH 62512 (NIMH NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | null | IM | 22705481, acs055, 10.1093/arclin/acs055, PMC3399510, 20686138, 5349366, 12959507, 3415427, 20603743, 11083161, 21544705, 9375218, 11253838, 17716600, 15939969, 12169339, 3014994, 11483137, 15512922, 18211160, 12297609, 1627973, 11365209, 17209696, 1756340, 16567037, 8845700, 7996652, 10824505, 17289343, 12030306, 9706540, 11329393, 15147590, 16251836, 17308225, 19000286, 1430852, 15746491, 8352676, 18695060, 19472057, 7991117, 18045694, 17694436, 23037647, 8422087, 22114912, 12724007, 10649549, 15746478 | Apathy is a relatively common clinical feature of HIV-Associated Neurocognitive Disorders, but little is known about its implications for everyday functioning outcomes. In the present study, we examined the associations between apathy and self-reported instrumental activities of daily living (IADL) and neurocognitive complaints in 75 participants with HIV infection and 52 demographically comparable seronegative comparison subjects. All volunteers completed the apathy subscale of the Frontal Systems Behavioral Scale as part of a comprehensive neuromedical, psychiatric, and neurocognitive research evaluation. When compared with the seronegative comparison participants, the HIV+ group reported significantly higher current levels of apathy, but did not differ in self-report of prior (i.e., pre-seroconversion) apathy. Higher current apathy self-ratings were associated with greater severity of IADL declines and more numerous cognitive complaints in the HIV+ sample, even after adjusting for potential psychiatric (e.g., depression), medical (e.g., hepatitis C co-infection), and neurocognitive predictors. Cognitive complaints, but not IADLs, were also uniquely associated with ratings of executive dysfunction and disinhibition. All told, these findings suggest that apathy may make a unique contribution to important everyday functioning outcomes among persons living with HIV infection. The clinical detection of apathy may help identify HIV-infected individuals at particular risk for functional impairments who may require additional support to maintain independence. | Activities of Daily Living, Adult, Apathy, Depression, Female, HIV Infections, Humans, Male, Middle Aged, Neuropsychological Tests, Self Report, Surveys and Questionnaires | null |
22,705,486 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Carotenoid-based coloration, condition, and immune responsiveness in the nestlings of a sexually dimorphic bird of prey. | Sternalski Audrey, Mougeot François, Pérez-Rodríguez Lorenzo, Bretagnolle Vincent | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Phytohemagglutinins, Carotenoids | IM | 22705486, 10.1086/665981 | In many birds, nestlings exhibit brightly colored traits that are pigmented by carotenoids. Carotenoids are diet limited and also serve important health-related physiological functions. The proximate mechanisms behind the expression of these carotenoid-pigmented traits are still poorly known, especially in nestlings with sexual size dimorphism. In these nestlings, intrabrood competition levels and growth strategies likely differ between sexes, and this may in turn influence carotenoid allocation rules. We used dietary carotenoid supplementation to test whether wild marsh harrier (Circus aeruginosus) nestlings were carotenoid limited and whether carotenoid allocation strategies varied between sexes, which differ in their size and growth strategies. When supplemented, nestlings used the supplemental carotenoids to increase their coloration independently of their sex. We showed that the condition dependence of the carotenoid level and the response to an immune challenge (phytohemagglutinin test) differed between sexes, possibly because sexual size dimorphism influences growth strategies and/or intrabrood competition levels and access to different types of food. In this species, which often feeds on mammals, a trade-off likely exists between food quantity (energy) and quality (carotenoid content). Finally, carotenoid-based coloration expressed in marsh harrier nestlings appeared to be indicative of immune responsiveness rather than condition, therefore potentially advertising to parents nestling quality or value rather than nutritional need. | Animal Feed, Animals, Body Composition, Carotenoids, Falconiformes, Female, France, Hemagglutination, Male, Phytohemagglutinins, Pigmentation, Random Allocation, Sex Characteristics, Time Factors | null |
22,705,484 | 2012-10-18 | 2013-11-21 | 1537-5293 | Physiological and biochemical zoology : PBZ | Relationships between hair melanization, glutathione levels, and senescence in wild boars. | Galván Ismael, Alonso-Alvarez Carlos, Negro Juan J | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Melanins, Thiobarbiturates, pheomelanin, eumelanin, Glutathione, thiobarbituric acid | IM | 22705484, 10.1086/666606 | The synthesis of melanins, which are the most common animal pigments, is influenced by glutathione (GSH), a key intracellular antioxidant. At high GSH levels, pheomelanin (the lightest melanin form) is produced, whereas production of eumelanin (the darkest melanin form) does not require GSH. Oxidative damage typically increases with age, and age-related decreases in GSH have accordingly been found in diverse organisms. Therefore, there should be positive associations between the capacity to produce eumelanic traits, GSH levels, and senescence, whereas there should be negative associations between the capacity to produce pheomelanic traits, GSH levels, and senescence. We explored this hypothesis in a free-ranging population of wild boars Sus scrofa of different ages. As expected from the fact that pheomelanogenesis consumes GSH, levels of this antioxidant in muscle tended to be negatively related to pheomelanization and positively related to eumelanization in pelage, and the degree of pelage pheomelanization was positively related to oxidative damage as reflected by levels of thiobarbituric-acid-reactive substances (TBARS), which is consistent with the hypothesis that pheomelanin synthesis has physiological costs. In our cross-sectional sample, GSH levels did not show senescence effects, and we did not detect senescence effects in pelage melanization. Prime body condition and low TBARS levels were also associated with hair graying, which is attributable to a loss of melanin produced by oxidative stress, thus raising the possibility that hair graying constitutes a signal of resistance to oxidative stress in wild boars. Our results suggest that the degree of melanization is linked to GSH levels in wild boars and that their antioxidant damage shows senescence effects. | Aging, Animals, Female, Glutathione, Hair, Linear Models, Lipid Peroxidation, Male, Melanins, Models, Animal, Muscle, Skeletal, Oxidation-Reduction, Oxidative Stress, Spectrophotometry, Sus scrofa, Thiobarbiturates | null |
22,705,482 | 2012-10-18 | 2013-11-21 | 1537-5293 | Physiological and biochemical zoology : PBZ | The influence of social status on hepatic glucose metabolism in rainbow trout Oncorhynchus mykiss. | Gilmour Kathleen M, Kirkpatrick Sheryn, Massarsky Andrey, Pearce Brenda, Saliba Sarah, Stephany Céleste-Élise, Moon Thomas W | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Liver Glycogen, RNA, Messenger, Receptors, Adrenergic, beta, Glycogen Phosphorylase, Glucose, Hydrocortisone | IM | 22705482, 10.1086/666497 | The effects of chronic social stress on hepatic glycogen metabolism were examined in rainbow trout Oncorhynchus mykiss by comparing hepatocyte glucose production, liver glycogen phosphorylase (GP) activity, and liver β-adrenergic receptors in dominant, subordinate, control, fasted, and cortisol-treated fish. Hepatocyte glucose production in subordinate fish was approximately half that of dominant fish, reflecting hepatocyte glycogen stores in subordinate trout that were just 16% of those in dominant fish. Fasting and/or chronic elevation of cortisol likely contributed to these differences based on similarities among subordinate, fasted, and cortisol-treated fish. However, calculation of the "glycogen gap"--the difference between glycogen stores used and glucose produced--suggested an enhanced gluconeogenic potential in subordinate fish that was not present in fasted or cortisol-treated trout. Subordinate, fasted, and cortisol-treated trout also exhibited similar GP activities (both total activity and that of the active or a form), and these activities were in all cases significantly lower than those in control trout, perhaps reflecting an attempt to protect liver glycogen stores or a modified capacity to activate GP. Dominant trout exhibited the lowest GP activities (20%-24% of the values in control trout). Low GP activities, presumably in conjunction with incoming energy from feeding, allowed dominant fish to achieve the highest liver glycogen concentrations (double the value in control trout). Liver membrane β-adrenoceptor numbers (assessed as the number of (3)H-CGP binding sites) were significantly lower in subordinate than in dominant trout, although this difference did not translate into attenuated adrenergic responsiveness in hepatocyte glucose production in vitro. Transcriptional regulation, likely as a result of fasting, was indicated by significantly lower β(2)-adrenoceptor relative mRNA levels in subordinate and fasted trout. Collectively, the data indicate that social status shapes liver metabolism and in particular glycogen metabolism, favoring accumulation of glycogen reserves from incoming energy in dominant fish and reliance on onboard fuels in subordinate fish. | Animals, Female, Glucose, Glycogen Phosphorylase, Hepatocytes, Hydrocortisone, Liver, Liver Glycogen, Oncorhynchus mykiss, RNA, Messenger, Real-Time Polymerase Chain Reaction, Receptors, Adrenergic, beta, Reverse Transcriptase Polymerase Chain Reaction, Social Dominance | null |
22,705,483 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Hybridization in sunfish influences the muscle metabolic phenotype. | Davies R, Mathers K E, Hume A D, Bremer K, Wang Y, Moyes C D | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Mitochondrial, Electron Transport Chain Complex Proteins, Fish Proteins | IM | 22705483, 10.1086/666058 | Hybridization has the potential to exert pleiotropic effects on metabolism. Effects on mitochondrial enzymes may arise through incompatibilities in nuclear- and mitochondrial-encoded subunits of the enzyme complexes of oxidative phosphorylation. We explored the metabolic phenotype of bluegill (Lepomis macrochirus), pumpkinseed (Lepomis gibbosus), and their unidirectional F(1) hybrids (male bluegill × female pumpkinseed). In hybrids, glycolytic enzyme activities were indistinguishable from (aldolase, pyruvate kinase) or intermediate to (lactate dehydrogenase, phosphoglucoisomerase) parentals, but complex IV activities aligned with pumpkinseed, both 30% lower than bluegill. In isolated mitochondria, the specific activities of complexes I, II, and V were indistinguishable between groups. However, both complex III and IV showed indications of depressed activities in hybrid mitochondria, though no effects on mitochondrial state 3 or state 4 respiration were apparent. The patterns in complex IV activities were due to differences in enzyme content rather than enzyme V(max); immunoblots comparing complex IV content with catalytic activity were indistinguishable between groups. The sequence differences in complex IV catalytic subunits (CO1, CO2, CO3) were minor in nature; however, the mtDNA-encoded subunit of complex III (cytochrome b) showed eight differences between bluegill and pumpkinseed, several of which could have structural consequences to the multimeric enzyme, contributing to the depressed complex III catalytic activity in hybrids. | Animals, Base Sequence, DNA, Mitochondrial, Electron Transport Chain Complex Proteins, Energy Metabolism, Female, Fish Proteins, Hybridization, Genetic, Male, Mitochondria, Molecular Sequence Data, Muscle, Skeletal, Oxidative Phosphorylation, Perciformes, Phenotype, Polymerase Chain Reaction, Sequence Alignment | null |
22,705,492 | 2012-11-20 | 2020-03-11 | 1873-5177 | Pharmacology, biochemistry, and behavior | Melanin-concentrating hormone receptor 1 (MCH1-R) antagonism: reduced appetite for calories and suppression of addictive-like behaviors. | Karlsson Camilla, Zook Michelle, Ciccocioppo Roberto, Gehlert Donald R, Thorsell Annika, Heilig Markus, Cippitelli Andrea | eng | null | Journal Article | 6-(4-chlorophenyl)-3-(3-methoxy-4-(2-pyrrolidin-1-ylethoxy)phenyl)-3H-thieno(3,2-d)pyrimidin-4-one, Carbohydrates, MCHR1 protein, rat, Pyrimidinones, Receptors, Somatostatin, Thiophenes, Saccharin | IM | 22705492, S0091-3057(12)00165-7, 10.1016/j.pbb.2012.06.010 | The hypothalamic neuropeptide melanin-concentrating hormone and its MCH1 receptor have been implicated in regulation of feeding and energy homeostasis, as well as modulation of reward-related behaviors. Here, we examined whether the MCH system plays a role both in caloric and motivational aspects of sugar intake. | Animals, Appetite, Behavior, Addictive, Carbohydrates, Conditioning, Operant, Cues, Energy Intake, Environment, Male, Motivation, Pyrimidinones, Rats, Rats, Wistar, Receptors, Somatostatin, Recurrence, Reinforcement Schedule, Saccharin, Self Administration, Thiophenes | null |
22,705,485 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Carotenoid-based ornaments of female and male American goldfinches (Spinus tristis) show sex-specific correlations with immune function and metabolic rate. | Kelly Ryan J, Murphy Troy G, Tarvin Keith A, Burness Gary | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antibodies, Anti-Idiotypic, IgY, Immunoglobulins, Carotenoids | IM | 22705485, 10.1086/666059 | Conspicuous ornamentation has been linked to immunological and physiological condition in males of many species. In species where both sexes are ornamented, it is unclear whether the signal content of ornaments differs between males and females. We examined the immunological and physiological correlates of carotenoid-based bill and plumage ornamentation in American goldfinches Spinus tristis, a species in which bright orange bills are sexually monomorphic but yellow plumage is sexually dimorphic during the breeding season. Because bill color is dynamic over short periods while plumage color is static over longer time frames, we tested whether these signals have the potential to provide temporal information about immunity and condition. In both sexes, bill color (but not plumage color) was negatively related to leukocyte differential, a measure of recent stress, while plumage color (but not bill color) was positively related to resting metabolic rate. In females, bill color also positively correlated with immunoglobulin Y, a component of acquired immunity, while plumage color positively predicted natural antibody levels, a component of innate immunity. In males, neither bill color nor plumage color predicted immune function, suggesting that the mechanisms underlying these signals vary with sex. Our results demonstrate that dynamic signals such as bill coloration do not merely reflect the same information provided by static signals but that these two classes of signal provide information about different temporal aspects of phenotypic quality. Furthermore, our results indicate that a signal expressed in both sexes has the potential to provide different information depending on the sex of the bearer. | Adaptive Immunity, Animal Communication, Animals, Antibodies, Anti-Idiotypic, Basal Metabolism, Beak, Body Composition, Carotenoids, Enzyme-Linked Immunosorbent Assay, Feathers, Female, Finches, Hemagglutination, Immunity, Innate, Immunoglobulins, Leukocytes, Male, Ontario, Pigmentation, Sex Distribution, Time Factors | null |
22,705,494 | 2012-09-12 | 2015-11-19 | 1538-7488 | The American journal of nursing | Facilitators and barriers to clinical practice guideline use among nurses. | Abrahamson Kathleen A, Fox Rebekah L, Doebbeling Bradley N | eng | null | Journal Article, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22705494, 10.1097/01.NAJ.0000415957.46932.bf | Clinical practice guidelines, which are designed to encourage consistent, efficient applications of scientific evidence in the daily practice of clinicians, are often underutilized. The majority of research concerning their implementation and use has focused on the work of physicians; more research concerning their use by nurses is needed. | Guideline Adherence, Health Care Surveys, Health Knowledge, Attitudes, Practice, Humans, Practice Guidelines as Topic, Practice Patterns, Nurses', United States, United States Department of Veterans Affairs | null |
22,705,495 | 2012-09-12 | 2013-05-20 | 1538-7488 | The American journal of nursing | Therapeutic hypothermia after cardiac arrest. | Erb Jessica L, Hravnak Marilyn, Rittenberger Jon C | eng | null | Journal Article | null | IM | 22705495, 10.1097/01.NAJ.0000415959.85050.1a | Irreversible brain damage and death are common outcomes after cardiac arrest, even when resuscitation is initially successful. Chances for both survival and a good neurologic outcome are improved when mild hypothermia is induced shortly after reperfusion. Unfortunately, this treatment is often omitted from advanced cardiac life support protocols. The authors discuss the efficacy of therapeutic hypothermia, indications and contraindications for its use, various induction methods, associated complications and adverse effects, and nursing care specific to patients undergoing this procedure. | Advanced Cardiac Life Support, Heart Arrest, Humans, Hypothermia, Induced, Hypoxia-Ischemia, Brain, Reperfusion Injury | null |
22,705,490 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Dietary lipid saturation influences environmental temperature preference but not resting metabolic rate in the Djungarian Hamster (Phodopus sungorus). | Pannorfi Ryan, Zee Barry M, Vatnick Itzick, Berner Nancy, Hiebert Sara M | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Dietary Fats, Fatty Acids, Fatty Acids, Unsaturated | IM | 22705490, 10.1086/666473 | Heterothermic rodents increase self-selection of diets rich in polyunsaturated fatty acids (PUFAs) when exposed to cold, short days, or short-day melatonin profiles, and Djungarian hamsters (Phodopus sungorus) do so in long days in response to cold exposure alone. To determine whether Djungarian hamsters are also capable of selecting a thermal environment in response to dietary lipid composition, continuously normothermic hamsters were fed either a PUFA-rich diet or a diet rich in saturated fatty acids (SFAs) for 6-10 wk and given a choice of thermal environments. As predicted, SFA-fed hamsters were more likely than PUFA-fed hamsters to occupy the single heated corner of their cage ([Formula: see text]) and were most likely to show this diet-related difference in behavior when T(a) fell within the thermal neutral zone. Respirometry revealed no effect of diet on whole-animal or mass-specific resting metabolic rate or on lower critical temperature. The results are more consistent with the homeoviscous adaptation hypothesis, which predicts that organisms should make physiological and/or behavioral adjustments that preserve membrane fluidity within a relatively small range, than with the membrane pacemaker hypothesis, which predicts that high PUFA content in membrane phospholipids should increase basal metabolic rate. | Acclimatization, Animals, Basal Metabolism, Cricetinae, Dietary Fats, Fatty Acids, Fatty Acids, Unsaturated, Microclimate, Oxygen Consumption, Phodopus, Random Allocation, Seasons, Temperature | null |
22,705,488 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | A test of bone mobilization relative to reproductive demand: skeletal quality is improved in cannibalistic females with large litters. | Hood Wendy R | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Minerals | IM | 22705488, 10.1086/666057 | In species with repeated bouts of reproduction, a female's ability to retain sufficient tissue for self-maintenance is essential to her survival and capacity for future reproduction. Loss of bone mineral content results in bone fragility and the possibility of reduced survival, so females should guard against the overuse of their bone mineral during reproduction. Given these constraints, I predicted that bone mobilization would increase with litter size in mice but plateau before maximum litter size was reached. To test this idea, I manipulated the litter sizes of house mice on the day of parturition to 3, 8, 13, and 18 offspring. At weaning, I euthanized the females and calculated whole-body and bone mineral composition. The total mineral content of females' femurs dropped as litter size increased to the average litter size for this strain of mouse (13) but surprisingly, femoral mineral content was higher for females assigned the largest litter sizes (18). Seven of the nine females assigned 18 young cannibalized some of their offspring. For females assigned to these larger litters, femoral ash content was not correlated with number of young consumed, suggesting that mineral recycling had little effect on final bone mineral content. However, nursing effort (accounting for young lost to cannibalism) was correlated with maternal femoral ash at weaning. These finding suggest that the high bone mineral content of females assigned the largest litters was associated with a reduction in endogenous mineral allocated to the litter. | Animals, Bone Density, Cannibalism, Female, Femur, Litter Size, Mice, Mice, Inbred ICR, Minerals, Osteogenesis, Random Allocation | null |
22,705,487 | 2012-10-18 | 2013-11-21 | 1537-5293 | Physiological and biochemical zoology : PBZ | Factors influencing the turnover and net isotopic discrimination of hydrogen isotopes in proteinaceous tissue: experimental results using Japanese quail. | Storm-Suke Andrea, Norris D Ryan, Wassenaar Leonard I, Chin Eunice, Nol Erica | eng | null | Journal Article | Deuterium | IM | 22705487, 10.1086/666476 | Stable hydrogen isotopes (δ(2)H) are commonly used in studies of animal movement. Tissue that is metabolically inactive after growth (e.g., feathers) provides spatial or dietary information that reflects only the period of tissue growth, whereas tissues that are metabolically active (e.g., red blood cells) provide a moving window of forensic information. However, using δ(2)H for studies of animal movement relies on the assumption that tissue δ(2)H values reflect dietary δ(2)H values, plus or minus a net diet-tissue discrimination value, and that the turnover rate is known for metabolically active tissue. The metabolic rate of an animal may influence both diet-tissue discrimination values and isotopic tissue turnover rate, but this hypothesis has not been tested experimentally. To examine the metabolic hypothesis, an experimental group of 12 male and 15 female captive Japanese quail (Coturnix japonica) was housed at 8.9°C for 90 d to elevate their metabolic rates (mL CO(2) min(-1)), and a control group of 12 male and 13 female quail was housed at room temperature during the same period. For both experimental and control birds, diet-tissue discrimination values were estimated for red blood cells and feathers. To determine turnover rate, experimental and control birds were switched from a (2)H-enriched diet to a (2)H-depleted diet, with red blood cells sampled before and after diet switch. Metabolic rate did not influence red blood cell hydrogen isotope turnover rate (η(2)(p) = 0.24)) or diet-feather isotope discrimination values (η(2)(p) = 0.86). Diet-feather hydrogen isotopic discrimination had a significant sex plus treatment interaction effect; female feathers were depleted in (2)H relative to food regardless of treatment, whereas male feathers were enriched in (2)H. The effect of sex suggested that experimental studies should examine whether coeval males and females differ in blood δ(2)H levels during certain periods of the annual cycle. | Animal Feed, Animals, Basal Metabolism, Cold Temperature, Coturnix, Deuterium, Erythrocytes, Feathers, Female, Male, Random Allocation, Sex Distribution, Tissue Distribution | null |
22,705,489 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Hibernation and circadian rhythms of body temperature in free-living Arctic ground squirrels. | Williams Cory T, Barnes Brian M, Richter Melanie, Buck C Loren | eng | null | Journal Article, Research Support, U.S. Gov't, Non-P.H.S. | null | IM | 22705489, 10.1086/666509 | In mammals, the circadian master clock generates daily rhythms of body temperature (T(b)) that act to entrain rhythms in peripheral circadian oscillators. The persistence and function of circadian rhythms during mammalian hibernation is contentious, and the factors that contribute to the reestablishment of rhythms after hibernation are unclear. We collected regular measures of core T(b) (every 34 min) and ambient light conditions (every 30 s) before, during, and following hibernation in free-living male arctic ground squirrels. Free-running circadian T(b) rhythms at euthermic levels of T(b) persisted for up to 10 d in constant darkness after animals became sequestered in their hibernacula in fall. During steady state torpor, T(b) was constant and arrhythmic for up to 13 d (within the 0.19°C resolution of loggers). In spring, males ended heterothermy but remained in their burrows at euthermic levels of T(b) for 22-26 d; patterns of T(b) were arrhythmic for the first 10 d of euthermia. One of four squirrels exhibited a significant free-running T(b) rhythm (τ = 22.1 h) before emergence; this squirrel had been briefly exposed to low-amplitude light before emergence. In all animals, diurnal T(b) rhythms were immediately reestablished coincident with emergence to the surface and the resumption of surface activity. Our results support the hypothesis that clock function is inhibited during hibernation and reactivated by exposure to light, although resumption of extended surface activity does not appear to be necessary to reinitiate T(b) cycles. | Alaska, Animals, Arctic Regions, Body Temperature, Circadian Clocks, Circadian Rhythm, Hibernation, Light, Male, Remote Sensing Technology, Sciuridae, Seasons, Time Factors | null |
22,705,493 | 2012-11-20 | 2022-03-16 | 1873-5177 | Pharmacology, biochemistry, and behavior | Ovarian hormones and chronic administration during adolescence modify the discriminative stimulus effects of delta-9-tetrahydrocannabinol (Δ⁹-THC) in adult female rats. | Winsauer Peter J, Filipeanu Catalin M, Bailey Evangeline M, Hulst Jerielle L, Sutton Jessie L | eng | 8P20GM103514-10 (NIGMS NIH HHS, United States); R01 DA019625 (NIDA NIH HHS, United States); DA019625 (NIDA NIH HHS, United States); R03 DA031596 (NIDA NIH HHS, United States); DA031596 (NIDA NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Cyclic AMP Response Element-Binding Protein, Gonadal Steroid Hormones, Hallucinogens, Receptor, Cannabinoid, CB1, Dronabinol, Extracellular Signal-Regulated MAP Kinases | IM | 22705493, S0091-3057(12)00163-3, 10.1016/j.pbb.2012.06.008, PMC3402337, NIHMS389698, 8388551, 5950056, 11080200, 19156848, 16965297, 16714325, 8380864, 15919107, 11102504, 9776133, 17581536, 1273591, 9888629, 951454, 12657697, 11598379, 15252015, 11553284, 19912468, 7538581, 15913574, 7562536, 10719081, 18172430, 12492298, 16954596, 7675962, 8739546, 8856831, 17681354, 21781118, 21158010, 10582605, 11224227, 8289577, 1323651, 10780256, 17161830, 10436037, 18446018, 17967938, 1313164, 15582014, 10733937 | Marijuana abuse during adolescence may alter its abuse liability during adulthood by modifying the interoceptive (discriminative) stimuli produced, especially in females due to an interaction with ovarian hormones. To examine this possibility, either gonadally intact or ovariectomized (OVX) female rats received 40 intraperitoneal injections of saline or 5.6 mg/kg of Δ⁹-THC daily during adolescence, yielding 4 experimental groups (intact/saline, intact/Δ⁹-THC, OVX/saline, and OVX/Δ⁹-THC). These groups were then trained to discriminate Δ⁹-THC (0.32-3.2 mg/kg) from saline under a fixed-ratio (FR) 20 schedule of food presentation. After a training dose was established for the subjects in each group, varying doses of Δ⁹-THC were substituted for the training dose to obtain dose-effect (generalization) curves for drug-lever responding and response rate. The results showed that: 1) the OVX/saline group had a substantially higher mean response rate under control conditions than the other three groups, 2) both OVX groups had higher percentages of THC-lever responding than the intact groups at doses of Δ⁹-THC lower than the training dose, and 3) the OVX/Δ⁹-THC group was significantly less sensitive to the rate-decreasing effects of Δ⁹-THC compared to other groups. Furthermore, at sacrifice, western blot analyses indicated that chronic Δ⁹-THC in OVX and intact females decreased cannabinoid type-1 receptor (CB1R) levels in the striatum, and decreased phosphorylation of cyclic adenosine monophosphate response element binding protein (p-CREB) in the hippocampus. In contrast to the hippocampus, chronic Δ⁹-THC selectively increased p-CREB in the OVX/saline group in the striatum. Extracellular signal-regulated kinase (ERK) was not significantly affected by either hormone status or chronic Δ⁹-THC. In summary, these data in female rats suggest that cannabinoid abuse by adolescent human females could alter their subsequent responsiveness to cannabinoids as adults and have serious consequences for brain development. | Animals, Blotting, Western, Conditioning, Operant, Corpus Striatum, Cyclic AMP Response Element-Binding Protein, Data Interpretation, Statistical, Discrimination Learning, Discrimination, Psychological, Dose-Response Relationship, Drug, Dronabinol, Extracellular Signal-Regulated MAP Kinases, Female, Gonadal Steroid Hormones, Hallucinogens, Hippocampus, Ovariectomy, Ovary, Psychomotor Performance, Rats, Rats, Long-Evans, Receptor, Cannabinoid, CB1, Reinforcement Schedule | null |
22,705,497 | 2013-05-03 | 2021-10-21 | 1523-6536 | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | Transplantation for autoimmune diseases in north and South America: a report of the Center for International Blood and Marrow Transplant Research. | Pasquini Marcelo C, Voltarelli Julio, Atkins Harold L, Hamerschlak Nelson, Zhong Xiaobo, Ahn Kwang Woo, Sullivan Keith M, Carrum George, Andrey Jeffrey, Bredeson Christopher N, Cairo Mitchell, Gale Robert Peter, Hahn Theresa, Storek Jan, Horowitz Mary M, McSweeney Peter A, Griffith Linda M, Muraro Paolo A, Pavletic Steven Z, Nash Richard A | eng | 5U01HL069294 (NHLBI NIH HHS, United States); U24 CA076518 (NCI NIH HHS, United States); HHSH234200637015C (PHS HHS, United States); U24-CA76518 (NCI NIH HHS, United States); U01 HL069294 (NHLBI NIH HHS, United States) | Journal Article, Multicenter Study, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S. | null | IM | 22705497, S1083-8791(12)00237-6, 10.1016/j.bbmt.2012.06.003, PMC3950896, NIHMS532549, 12763935, 10907647, 17646844, 19895895, 19186105, 9383225, 12586609, 16732546, 19433668, 18314435, 19741229, 22127896, 9156239, 17426276, 19773265, 22226104, 9256315, 10520015, 8896432, 22002489, 12931249, 11781654, 15738052, 15334459, 18565456, 19741227, 12176878, 15765390, 19139309, 19139739, 20304084, 17452515, 12393477, 16275589, 19855441, 19584827, 21157646, 11350846, 14994391, 9543056, 16449618, 21328309, 9324027, 15765114, 11071262, 21835965 | Hematopoietic cell transplantation (HCT) is an emerging therapy for patients with severe autoimmune diseases (AID). We report data on 368 patients with AID who underwent HCT in 64 North and South American transplantation centers reported to the Center for International Blood and Marrow Transplant Research between 1996 and 2009. Most of the HCTs involved autologous grafts (n = 339); allogeneic HCT (n = 29) was done mostly in children. The most common indications for HCT were multiple sclerosis, systemic sclerosis, and systemic lupus erythematosus. The median age at transplantation was 38 years for autologous HCT and 25 years for allogeneic HCT. The corresponding times from diagnosis to HCT were 35 months and 24 months. Three-year overall survival after autologous HCT was 86% (95% confidence interval [CI], 81%-91%). Median follow-up of survivors was 31 months (range, 1-144 months). The most common causes of death were AID progression, infections, and organ failure. On multivariate analysis, the risk of death was higher in patients at centers that performed fewer than 5 autologous HCTs (relative risk, 3.5; 95% CI, 1.1-11.1; P = .03) and those that performed 5 to 15 autologous HCTs for AID during the study period (relative risk, 4.2; 95% CI, 1.5-11.7; P = .006) compared with patients at centers that performed more than 15 autologous HCTs for AID during the study period. AID is an emerging indication for HCT in the region. Collaboration of hematologists and other disease specialists with an outcomes database is important to promote optimal patient selection, analysis of the impact of prognostic variables and long-term outcomes, and development of clinical trials. | Adolescent, Adult, Autoimmune Diseases, Child, Child, Preschool, Disease-Free Survival, Female, Hematopoietic Stem Cell Transplantation, Humans, Longitudinal Studies, Male, Middle Aged, North America, Prognosis, South America, Transplantation, Autologous, Transplantation, Homologous, Treatment Outcome | null |
22,705,498 | 2012-10-26 | 2012-06-19 | 1361-6528 | Nanotechnology | Enhancement in broadband and quasi-omnidirectional antireflection of nanopillar arrays by ion milling. | Huang Zhifeng, Hawkeye Matthew M, Brett Michael J | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Ions | IM | 22705498, 10.1088/0957-4484/23/27/275703 | A new technique is developed to fabricate biomimetic antireflection coatings (ARCs). This technique combines a bottom-up fabrication approach (glancing angle deposition, or GLAD) with a top-down engineering process (ion milling). The GLAD technique is first utilized to produce nanopillar arrays (NPAs) with broadened structures, which are subsequently transformed into biomimetic tapered geometries by means of post-deposition ion milling. This structure transformation, due to milling-induced mass redistribution, remarkably decreases reflection over a wide wavelength range (300-1700 nm) and field of view (angle of incidence < 60° with respect to the substrate normal). The milling-induced antireflection enhancement has been demonstrated in the NPAs made of Si, SiO(x) and TiO(2), illustrating that this integrated technique is readily adapted to a wide variety of materials. Good agreement between simulation and experiment indicates that the enhanced antireflection performance is ascribed to a smoother refractive index transition from the substrate to the air, which improves the impedance match and reduces reflection losses. Additionally, ion bombardment tends to alter the stoichiometry and diminish the crystallographic structure of the NPA materials. The broadband and quasi-omnidirectional antireflection observed establishes the strong competitiveness of this technique with the methods previously reported. | Ions, Light, Materials Testing, Nanostructures, Particle Size, Refractometry, Scattering, Radiation, Surface Plasmon Resonance, Surface Properties | null |
22,705,491 | 2012-10-18 | 2012-06-18 | 1537-5293 | Physiological and biochemical zoology : PBZ | Of the importance of metabolic phases in the understanding of oxidative stress in prolonged fasting and refeeding. | Sylvie Geiger, Marion Kauffmann, Yvon Le Maho, Jean-Patrice Robin, Criscuolo Francois | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Antioxidants | IM | 22705491, 10.1086/666364 | Life phases such as migration or reproduction may partly (or totally) prohibit food accessibility, making regulation of the energy balance one of the main challenges faced by wild organisms. Although long-term fasting is common in a number of species, it has been reported to be detrimental for the organism, notably because it induces oxidative stress. However, fasting metabolism is characterized by successive metabolic adaptations that are likely to be different in terms of stress, and no previous studies, to our knowledge, have tested the dynamics of changes in oxidative balance in relation to the metabolic phases of fasting. Our study provides a first insight into this relationship by inducing prolonged fasting and subsequent refeeding in captive mallards (Anas platyrhynchos). Both plasmatic antioxidant and oxidative damage levels were observed to be lower during fasting. Oxidative damage levels decreased by 95% during fasting, and they surprisingly remained at low levels even after 3 d of refeeding. In contrast, restoration of the antioxidant barrier was observed from the third day of refeeding onward, and it thus preceded the increase in oxidative damage levels. Therefore, under our experimental conditions, long-term fasting was not associated with marked oxidative stress in mallards. Because data interpretations may be influenced by concomitant metabolic states, we underline the importance of taking metabolic phases into account when studying oxidative stress during fasting. | Animals, Antioxidants, Body Weight, Ducks, Energy Metabolism, Fasting, Female, Oxidation-Reduction, Oxidative Stress | null |
22,705,499 | 2013-07-29 | 2013-11-21 | 1878-1519 | Respiratory physiology & neurobiology | Mechanisms and consequences of carbon dioxide sensing in fish. | Perry Steve F, Abdallah Sara | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Review | Carbon Dioxide, Carbonic Anhydrases | IM | 22705499, S1569-9048(12)00154-1, 10.1016/j.resp.2012.06.013 | Fish possess chemoreceptors able to sense increasing levels of ambient CO(2) and initiate various cardiorespiratory reflexes including hyperventilation and bradycardia. These chemoreceptors are localized predominantly to the gills, are oriented to sense the external environment and typically are stimulated by changes in environmental molecular CO(2) rather than H(+) (although increasing H(+) may be the proximate intracellular stimulus). In zebrafish, a subset of branchial neuroepithelial cells (NECs) act as bimodal sensors of CO(2) and O(2), similar to the Type I (glomus) cells of the mammalian carotid body. Like O(2) sensing, the mechanisms underlying CO(2) detection involve the inhibition of a background K(+) current leading to membrane depolarization and subsequent elevation of intracellular Ca(2+) levels. Carbonic anhydrase, by catalysing the hydration of CO(2) to H(+) and HCO(3)(-), appears to play a critical role in reducing NEC response times and increasing the magnitude of membrane depolarization accompanying hypercapnia. In larval zebrafish, CA activity is essential for the rapid development of hypercapnic bradycardia. | Animals, Carbon Dioxide, Carbonic Anhydrases, Fishes, Gills, Neuroepithelial Cells | null |
22,705,496 | 2013-04-02 | 2021-10-21 | 1876-7753 | Stem cell research | The abundance of Rad51 protein in mouse embryonic stem cells is regulated at multiple levels. | Tichy Elisia D, Pillai Resmi, Deng Li, Tischfield Jay A, Hexley Philip, Babcock George F, Stambrook Peter J | eng | R01 ES12695-4S1 (NIEHS NIH HHS, United States); R01 ES012695 (NIEHS NIH HHS, United States); P30 ES005022 (NIEHS NIH HHS, United States); P30 ES006096 (NIEHS NIH HHS, United States); ES011633 (NIEHS NIH HHS, United States); R01 ES016625 (NIEHS NIH HHS, United States); T32 ES007250 (NIEHS NIH HHS, United States); R01 ES011633 (NIEHS NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | E2F Transcription Factors, Proliferating Cell Nuclear Antigen, Rad51 Recombinase, Rad51 protein, mouse | IM | 22705496, S1873-5061(12)00058-X, 10.1016/j.scr.2012.05.004, PMC3412895, NIHMS380347, 15362224, 8628240, 1679766, 7913101, 19924215, 7559784, 7734951, 9153396, 19419956, 21593866, 9933638, 16303135, 20011546, 15322096, 11416145, 15252152, 17184172, 9330616, 15536187, 12676962, 2886367, 10224249, 8703992, 22056041, 20491544, 18570881, 2410919, 12138197, 12748277, 20368801, 20053681, 18055443, 18326858, 19650036, 20118940, 9694791, 15279789, 9152520, 20157510, 20413593, 11554292, 12447695, 12912992, 18462695, 15659392, 2861088, 8806438, 17142847, 14559999, 1531040, 19154734, 17110331, 15665856, 9038370, 10435585, 21076401, 21773671, 19156526, 20188668, 17512402, 8858149, 20446816 | DNA double-strand breaks (DSBs) in embryonic stem (ES) cells are repaired primarily by homologous recombination (HR). The mechanism by which HR is regulated in these cells, however, remains enigmatic. To gain insight into such regulatory mechanisms, we have asked how protein levels of Rad51, a key component of HR, are controlled in mouse ES cells and mouse embryo fibroblasts (MEFs). The Rad51 protein level is about 15-fold higher in ES cells than in MEFs. The level of Rad51 mRNA, however, is only ~2-fold higher, indicating that the differences in mRNA levels due to rates of transcription or mRNA stability are not sufficient to account for the large difference in the abundance of Rad51 protein. Comparison of Rad51 half-lives between ES cells and MEFs also did not explain the elevated level of Rad51 protein in the ES cells. A comparative assessment of the Rad51 translation level demonstrated that it is translated with much greater efficacy in ES cells than in MEFs. To determine whether this high level of translation in ES cells is a general phenomenon in these cells or whether it is a characteristic of specific proteins, such as those involved with recombination and cell cycle progression, we compared mechanisms that regulate the level of Pcna in ES cells with those that regulate Rad51. The half-life of Pcna and its rate of synthesis were considerably different from those of Rad51 in ES cells, demonstrating that regulation of Rad51 abundance cannot be generalized to other ES cell proteins and not to proteins involved in DNA replication and cell cycle control. Finally, we show that only a small proportion of the abundant Rad51 protein population is activated under basal conditions in ES cells and recruited to DNA DSBs and/or stalled replication forks. | Animals, DNA Breaks, Double-Stranded, DNA Repair, DNA Replication, E2F Transcription Factors, Embryo, Mammalian, Embryonic Stem Cells, Fibroblasts, Gene Expression Regulation, Mice, Proliferating Cell Nuclear Antigen, Protein Biosynthesis, Protein Stability, Rad51 Recombinase | null |
22,705,502 | 2012-11-13 | 2019-12-10 | 1873-6424 | Environmental pollution (Barking, Essex : 1987) | Estimating pesticide sampling rates by the polar organic chemical integrative sampler (POCIS) in the presence of natural organic matter and varying hydrodynamic conditions. | Charlestra Lucner, Amirbahman Aria, Courtemanch David L, Alvarez David A, Patterson Howard | eng | null | Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Organic Chemicals, Pesticides, Water Pollutants, Chemical | IM | 22705502, S0269-7491(12)00228-X, 10.1016/j.envpol.2012.05.001 | The polar organic chemical integrative sampler (POCIS) was calibrated to monitor pesticides in water under controlled laboratory conditions. The effect of natural organic matter (NOM) on the sampling rates (R(s)) was evaluated in microcosms containing <0.1-5 mg L(-1) of total organic carbon (TOC). The effect of hydrodynamics was studied by comparing R(s) values measured in stirred (SBE) and quiescent (QBE) batch experiments and a flow-through system (FTS). The level of NOM in the water used in these experiments had no effect on the magnitude of the pesticide sampling rates (p > 0.05). However, flow velocity and turbulence significantly increased the sampling rates of the pesticides in the FTS and SBE compared to the QBE (p < 0.001). The calibration data generated can be used to derive pesticide concentrations in water from POCIS deployed in stagnant and turbulent environmental systems without correction for NOM. | Environmental Monitoring, Hydrodynamics, Kinetics, Organic Chemicals, Pesticides, Water Pollutants, Chemical | null |
22,705,503 | 2012-11-13 | 2016-11-25 | 1873-6424 | Environmental pollution (Barking, Essex : 1987) | Atmospheric polybrominated diphenyl ethers (PBDEs) in the United Kingdom. | Birgul Askin, Katsoyiannis Athanasios, Gioia Rosalinda, Crosse John, Earnshaw Mark, Ratola Nuno, Jones Kevin C, Sweetman Andrew J | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Air Pollutants, Halogenated Diphenyl Ethers | IM | 22705503, S0269-7491(12)00232-1, 10.1016/j.envpol.2012.05.005 | The occurrence of PBDEs has been studied in the atmosphere of four sites in the United Kingdom over a period of ten years. The concentrations have exhibited a sharp decrease after 2001-2003. This is evident in the urban sites of Manchester and London and at the semi-rural site of Hazelrigg. The average ΣPBDE half-lives for these three sites were 3.4, 2.0 and 3.5 years respectively. ΣPBDEs concentrations in the UK (in 2010 ΣPBDEs < 10 pg m(-3)) are among the lowest reported in literature. Comparison of concentrations to estimated emissions and employment of PBDE profiles suggest that PBDEs in the UK atmosphere originate from primary emissions from products that contain mainly the penta-BDE technical mixture. The detection of BDE-183 in the majority of samples hints that octa-bromodiphenylether has also been used extensively in the UK, however to a smaller extent than the penta- product. | Air Pollutants, Environmental Monitoring, Halogenated Diphenyl Ethers, United Kingdom | null |
22,705,504 | 2012-11-13 | 2018-12-01 | 1873-6424 | Environmental pollution (Barking, Essex : 1987) | Associations between standardized school performance tests and mixtures of Pb, Zn, Cd, Ni, Mn, Cu, Cr, Co, and V in community soils of New Orleans. | Zahran Sammy, Mielke Howard W, Weiler Stephan, Hempel Lynn, Berry Kenneth J, Gonzales Christopher R | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Metals, Heavy, Soil Pollutants, Manganese | IM | 22705504, S0269-7491(12)00258-8, 10.1016/j.envpol.2012.05.019 | In New Orleans a strong inverse association was previously identified between community soil lead and 4th grade school performance. This study extends the association to zinc, cadmium, nickel, manganese, copper, chromium, cobalt, and vanadium in community soil and their comparative effects on 4th grade school performance. Adjusting for poverty, food security, racial composition, and teacher-student ratios, regression results show that soil metals variously reduce and compress student scores. Soil metals account for 22%-24% while food insecurity accounts for 29%-37% of variation in school performance. The impact on grade point averages were Ni > Co > Mn > Cu ~ Cr ~ Cd > Zn > Pb, but metals are mixtures in soils. The quantities of soil metal mixtures vary widely across the city with the largest totals in the inner city and smallest totals in the outer city. School grade point averages are lowest where the soil metal mixtures and food insecurity are highest. | Adolescent, Child, Cities, Female, Humans, Male, Manganese, Metals, Heavy, New Orleans, Schools, Soil Pollutants | null |
22,705,501 | 2012-10-24 | 2018-12-01 | 0006-3002 | Biochimica et biophysica acta | Cell surface binding and uptake of arginine- and lysine-rich penetratin peptides in absence and presence of proteoglycans. | Amand Helene L, Rydberg Hanna A, Fornander Louise H, Lincoln Per, Nordén Bengt, Esbjörner Elin K | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Carrier Proteins, Cell-Penetrating Peptides, Proteoglycans, Heparin, Arginine, penetratin, Lysine | IM | 22705501, S0005-2736(12)00194-0, 10.1016/j.bbamem.2012.06.006 | Cell surface proteoglycans (PGs) appear to promote uptake of arginine-rich cell-penetrating peptides (CPPs), but their exact functions are unclear. To address if there is specificity in the interactions of arginines and PGs leading to improved internalization, we used flow cytometry to examine uptake in relation to cell surface binding for penetratin and two arginine/lysine substituted variants (PenArg and PenLys) in wildtype CHO-K1 and PG-deficient A745 cells. All peptides were more efficiently internalized into CHO-K1 than into A745, but their cell surface binding was independent of cell type. Thus, PGs promote internalization of cationic peptides, irrespective of the chemical nature of their positive charges. Uptake of each peptide was linearly dependent on its cell surface binding, and affinity is thus important for efficiency. However, the gradients of these linear dependencies varied significantly. Thus each peptide's ability to stimulate uptake once bound to the cell surface is reliant on formation of specific uptake-promoting interactions. Heparin affinity chromatography and clustering experiments showed that penetratin and PenArg binding to sulfated sugars is stabilized by hydrophobic interactions and result in clustering, whereas PenLys only interacts through electrostatic attraction. This may have implications for the molecular mechanisms behind arginine-specific uptake stimulation as penetratin and PenArg are more efficiently internalized than PenLys upon interaction with PGs. However, PenArg is also least affected by removal of PGs. This indicates that an increased arginine content not only improve PG-dependent uptake but also that PenArg is more adaptable as it can use several portals of entry into the cell. | Animals, Arginine, CHO Cells, Carrier Proteins, Cell Membrane, Cell-Penetrating Peptides, Cricetinae, Cricetulus, Fluorescence, Heparin, Lysine, Protein Binding, Proteoglycans | null |
22,705,505 | 2012-11-19 | 2012-09-19 | 1873-2399 | Experimental hematology | Impact of anti-HLA antibodies on allogeneic hematopoietic stem cell transplantation outcomes after reduced-intensity conditioning regimens. | Detrait Marie, Dubois Valérie, Sobh Mohamad, Morisset Stéphane, Tedone Nathalie, Labussière Hélène, Gillis Lilia, Barraco Fiorenza, Cannas Giovanna, Ducastelle Sophie, Fatoum Jihane, Thomas Xavier, Chelgoum Youcef, Nicolini Franck-Emmanuel, Michallet Mauricette | eng | null | Clinical Trial, Journal Article | Autoantibodies, HLA Antigens | IM | 22705505, S0301-472X(12)00229-9, 10.1016/j.exphem.2012.06.003 | Anti-human leukocyte antigen (HLA) antibodies are associated with several complications in solid organ transplantations, but their impact after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is not yet well defined. To evaluate the relevance of anti-HLA antibodies, we have retrospectively analyzed 107 peripheral blood allo-HSCTs after reduced-intensity conditioning regimen between 2005 and 2010. Acute myeloid leukemia and multiple myeloma were the most frequent malignancies in the cohort. The detection of anti-HLA antibodies was systematically performed in all patients before transplantation. Anti-HLA antibodies were present in 24 patients (22%). There was no significant impact of anti-HLA antibodies on engraftment, incidence of relapse, and incidence of acute graft-vs-host disease. The presence of anti-HLA antibodies was associated with significantly worse overall survival (p = 0.006) and event-free survival (p = 0.024) after stratification on sex. The 3-year probability of overall survival was 34% without anti-HLA antibodies and 16% in their presence. Patients with anti-HLA antibodies had a higher transplant-related mortality associated with life-threatening vascular complications. Our study supports that anti-HLA antibodies should be tested and considered as an important impacting factor for transplantation outcomes after reduced-intensity conditioning allo-HSCT. We recommend its consideration before allo-HSCT in the donor-recipient selection parameters. | Acute Disease, Adult, Aged, Autoantibodies, Disease-Free Survival, Female, Graft vs Host Disease, HLA Antigens, Hematologic Neoplasms, Hematopoietic Stem Cell Transplantation, Humans, Incidence, Male, Middle Aged, Retrospective Studies, Survival Rate, Transplantation Conditioning, Transplantation, Homologous | null |
22,705,507 | 2012-11-05 | 2022-03-31 | 1873-2976 | Bioresource technology | Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source. | Abreu Ana P, Fernandes Bruno, Vicente António A, Teixeira José, Dragone Giuliano | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Industrial Waste, Lipids, Organic Chemicals, Pigments, Biological, Proteins, Carbon, Starch, Glucose, Galactose | IM | 22705507, S0960-8524(12)00799-7, 10.1016/j.biortech.2012.05.055 | Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic conditions. Moreover, supplementation of the inorganic culture medium with hydrolyzed cheese whey powder solution led to a significant improvement in microalgal biomass production and carbohydrate utilization when compared with the culture enriched with a mixture of pure glucose and galactose, due to the presence of growth promoting nutrients in cheese whey. Mixotrophic cultivation of C. vulgaris using the main dairy industry by-product could be considered a feasible alternative to reduce the costs of microalgal biomass production, since it does not require the addition of expensive carbohydrates to the culture medium. | Autotrophic Processes, Carbon, Cell Culture Techniques, Chlorella vulgaris, Dairying, Galactose, Glucose, Industrial Waste, Light, Lipids, Microalgae, Organic Chemicals, Pigments, Biological, Proteins, Starch | null |
22,705,508 | 2012-11-05 | 2018-12-01 | 1873-2976 | Bioresource technology | Methane recovery efficiency in a submerged anaerobic membrane bioreactor (SAnMBR) treating sulphate-rich urban wastewater: evaluation of methane losses with the effluent. | Giménez J B, Martí N, Ferrer J, Seco A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biofuels, Membranes, Artificial, Sulfates, Methane | IM | 22705508, S0960-8524(12)00762-6, 10.1016/j.biortech.2012.05.019 | The present paper presents a submerged anaerobic membrane bioreactor (SAnMBR) as a sustainable approach for urban wastewater treatment at 33 and 20 °C, since greenhouse gas emissions are reduced and energy recovery is enhanced. Compared to other anaerobic systems, such as UASB reactors, the membrane technology allows the use of biogas-assisted mixing which enhances the methane stripping from the liquid phase bulk. The methane saturation index obtained for the whole period (1.00±0.04) evidenced that the equilibrium condition was reached and the methane loss with the effluent was reduced. The methane recovery efficiency obtained at 20 °C (53.6%) was slightly lower than at 33 °C (57.4%) due to a reduction of the treatment efficiency, as evidenced by the lower methane production and the higher waste sludge per litre of treated wastewater. For both operational temperatures, the methane recovery efficiency was strongly affected by the high sulphate concentration in the influent wastewater. | Anaerobiosis, Biofuels, Biological Oxygen Demand Analysis, Bioreactors, Cities, Membranes, Artificial, Methane, Pilot Projects, Sulfates, Temperature, Waste Disposal, Fluid, Water Purification | null |
22,705,506 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Effect of adding rubber powder to poplar particles on composite properties. | Xu Min, Li Jian | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Powders, Resins, Synthetic, Rubber | IM | 22705506, S0960-8524(12)00292-1, 10.1016/j.biortech.2012.02.041 | The effect of adding rubber powder derived from waste tires to poplar wood particles on mechanical and water-resistant properties of particleboards was examined. Sixty panels were made with rubber contents of 0-40% at hot-pressing temperatures of 140-180 °C, methylene diphenyl diisocyanate resin contents of 2-6% and panel densities of 0.6 to 1 g cm(-3). Although the modulus of rupture (MOR), modulus of elasticity (MOE), internal bond (IB) strength were reduced by adding rubber powder, the thickness swelling (TS) was reduced by 7.3-61% when 10-40% rubber powder was added. Four regression equations (rubber content, pressing temperature, resin content and target panel density as functions of MOR, MOE, IB and TS) were developed and a nonlinear programing model was derived with operation research theory to obtain the most desirable panel properties under some production constraints. | Elastic Modulus, Materials Testing, Nonlinear Dynamics, Populus, Powders, Regression Analysis, Resins, Synthetic, Rubber, Temperature, Wood | null |
22,705,509 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Fate and effect of benzalkonium chlorides in a continuous-flow biological nitrogen removal system treating poultry processing wastewater. | Hajaya Malek G, Pavlostathis Spyros G | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Benzalkonium Compounds, Nitrogen | IM | 22705509, S0960-8524(12)00794-8, 10.1016/j.biortech.2012.05.050 | Quaternary ammonium compounds (QACs) are used for sanitation in many poultry processing facilities. This work investigated the fate and effect of a mixture of benzalkonium chlorides (BACs), a class of QACs widely used in commercial antimicrobial formulations, on the biological nitrogen removal (BNR) processes. A laboratory-scale BNR system was operated continuously for 670 days, fed with poultry processing wastewater amended with a mixture of BACs. Initially, the nitrogen removal efficiency deteriorated at a BAC feed concentration of 5 mg/L due to the complete inhibition of nitrification. However, after 27 days of operation, the system recovered and achieved 100% ammonia removal. High nitrogen removal efficiency was achieved even after the feed BAC concentration was stepwise increased up to 120 mg/L. Batch nitrification assays performed before, during, and after BAC exposure, showed that rapid microbial acclimation and BAC biodegradation contributed to the recovery of nitrification achieving efficient and stable long-term BNR system operation. | Aerobiosis, Air, Animals, Batch Cell Culture Techniques, Benzalkonium Compounds, Biodegradation, Environmental, Bioreactors, Denitrification, Fermentation, Nitrification, Nitrogen, Poultry, Rheology, Time Factors, Waste Disposal, Fluid, Water Purification | null |
22,705,510 | 2012-11-05 | 2017-11-16 | 1873-2976 | Bioresource technology | Regioselective enzymatic undecylenoylation of 8-chloroadenosine and its analogs with biomass-based 2-methyltetrahydrofuran as solvent. | Gao Wen-Li, Liu Huan, Li Ning, Zong Min-Hua | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Enzymes, Immobilized, Furans, Nucleosides, Solvents, 2-Chloroadenosine, Lipase, 2-methyltetrahydrofuran, 8-chloroadenosine | IM | 22705510, S0960-8524(12)00750-X, 10.1016/j.biortech.2012.04.104 | 2-Methyltetrahydrofuran (MeTHF), a biomass-derived compound, is a promising medium for biocatalysis and organometallic reactions. The regioselective acylation of 8-chloroadenosine (8-Cl-Ado) and its analogs was carried out in MeTHF with immobilized Penicillium expansum lipase. The lipase displayed more than twofold higher catalytic activity and much better thermostability in MeTHF than in other organic solvents and co-solvent systems. The optimum reaction medium, enzyme dosage, molar ratio of viny ester to nucleoside and reaction temperature for the enzymatic acylation of 8-Cl-Ado were MeTHF, 25 U/mL, 7.5 and 35 °C, respectively, under which the desirable 5'-O-undecylenoyl-8-Cl-Ado was obtained with a yield of 95% and a regioselectivity of >99% in 3 h. In addition, the lipase catalyzed regioselective undecylenoylation of other purine nucleosides, producing 5'-undecylenic acid esters with moderate to high yields (63-94%) and excellent 5'-regioselectivities (94->99%). Use of biomass-derived solvents might open up novel opportunities for sustainable and greener biocatalytic processes. | 2-Chloroadenosine, Biomass, Enzyme Stability, Enzymes, Immobilized, Furans, Lipase, Nucleosides, Penicillium, Solvents, Stereoisomerism, Substrate Specificity, Temperature | null |
22,705,512 | 2012-11-05 | 2022-04-09 | 1873-2976 | Bioresource technology | Evaluation of flocculants for the recovery of freshwater microalgae. | Granados M R, Acién F G, Gómez C, Fernández-Sevilla J M, Molina Grima E | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Electrolytes, EM 16, Thalidomide | IM | 22705512, S0960-8524(12)00761-4, 10.1016/j.biortech.2012.05.018 | The use flocculants on the recovery of freshwater microalgae is studied. Flocculants tested include metal salts, chitosan, and polyelectrolytes used in wastewater treatment processes. Influence of flocculant, but also the doses and biomass concentrations affecting biomass recovery as well as the concentration factor has been evaluated. Results showed that the use of metal salts or chitosan was not efficient, whereas polyelectrolytes allow the efficient recovery of biomass, at doses of 2-25 mg per gram of microalgae biomass. The required doses depend on the particular polyelectrolyte and the freshwater strain present; but cationic polyelectrolytes are generally recommended. The use of polyelectrolytes does not adversely affect water reuse in the production process. The concentration factors obtained are higher than 35 in most cases. Such high concentration factors allow a reduction in the equipment size necessary for biomass dewatering, thus improving the viability of using these microorganisms in biofuel or wastewater processes. | Biomass, Electrolytes, Flocculation, Fresh Water, Microalgae, Thalidomide | null |
22,705,511 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Integration of kinetic modeling and desirability function approach for multi-objective optimization of UASB reactor treating poultry manure wastewater. | Yetilmezsoy Kaan | eng | null | Journal Article | Manure, Sewage, Methane | IM | 22705511, S0960-8524(12)00841-3, 10.1016/j.biortech.2012.05.088 | An integrated multi-objective optimization approach within the framework of nonlinear regression-based kinetic modeling and desirability function was proposed to optimize an up-flow anaerobic sludge blanket (UASB) reactor treating poultry manure wastewater (PMW). Chen-Hashimoto and modified Stover-Kincannon models were applied to the UASB reactor for determination of bio-kinetic coefficients. A new empirical formulation of volumetric organic loading rate was derived for the first time for PMW to estimate the dimensionless kinetic parameter (K) in the Chen-Hashimoto model. Maximum substrate utilization rate constant and saturation constant were predicted as 11.83 g COD/L/day and 13.02 g COD/L/day, respectively, for the modified Stover-Kincannon model. Based on four process-related variables, three objective functions including a detailed bio-economic model were derived and optimized by using a LOQO/AMPL algorithm, with a maximum overall desirability of 0.896. The proposed optimization scheme demonstrated a useful tool for the UASB reactor to optimize several responses simultaneously. | Anaerobiosis, Animals, Biodegradation, Environmental, Bioreactors, Kinetics, Manure, Methane, Models, Theoretical, Poultry, Regression Analysis, Sewage, Waste Disposal, Fluid, Water Purification | null |
22,705,513 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Effects of microwave power and microwave irradiation time on pretreatment efficiency and characteristics of corn stover using combination of steam explosion and microwave irradiation (SE-MI) pretreatment. | Pang Feng, Xue Shulin, Yu Shengshuan, Zhang Chao, Li Bing, Kang Yong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Steam, Waste Products, Xylans, Cellulose, Lignin, Cellulase, Glucose | IM | 22705513, S0960-8524(12)00785-7, 10.1016/j.biortech.2012.05.041 | The effects of microwave power and microwave irradiation time on pretreatment efficiency and characteristics of corn stover were investigated based on a new process named combination of steam explosion and microwave irradiation (SE-MI) pretreatment. Results showed that with microwave power and microwave irradiation time increasing, glucose and xylose that released into hydrolyzate, as well as enzymatic hydrolysis yields and sugar yields of glucose and xylose were all slightly increased after SE-MI pretreatment. The maximum sugar yield was 72.1 g per 100 g glucose and xylose in feedstock, achieved at 540 W microwave power and 5 min microwave irradiation time. XRD analysis showed that the crystallinity of biomass was 15.6-19.9% lower for SE-MI pretreatment with microwave effect than that without microwave effect. However, low microwave power and short microwave irradiation time were favorable for SE-MI pretreatment considering energy consumption. | Biomass, Biotechnology, Cellulase, Cellulose, Crystallization, Glucose, Hydrolysis, Lignin, Microwaves, Spectroscopy, Fourier Transform Infrared, Steam, Time Factors, Waste Products, Xylans, Zea mays | null |
22,705,500 | 2013-06-12 | 2021-12-03 | 1872-6356 | Mechanisms of development | Regulation of Yorkie activity in Drosophila imaginal discs by the Hedgehog receptor gene patched. | Kagey Jacob D, Brown Jordan A, Moberg Kenneth H | eng | K12 GM000680 (NIGMS NIH HHS, United States); R01 CA123368 (NCI NIH HHS, United States); 2R01-CA123368 (NCI NIH HHS, United States) | Journal Article, Research Support, N.I.H., Extramural | Drosophila Proteins, Hedgehog Proteins, Intracellular Signaling Peptides and Proteins, MicroRNAs, Nuclear Proteins, Receptors, Cell Surface, Trans-Activators, YAP-Signaling Proteins, Yki protein, Drosophila, bantam microRNA, Drosophila, dark protein, Drosophila, dpp protein, Drosophila, ptc protein, Drosophila, hh protein, Drosophila, Protein Serine-Threonine Kinases, hpo protein, Drosophila | IM | 22705500, S0925-4773(12)00049-4, 10.1016/j.mod.2012.05.007, PMC3668547, NIHMS390180, 21376230, 17437995, 12142354, 16540507, 12196398, 12015606, 14704176, 10559939, 12021767, 19081070, 9693372, 11934850, 11171398, 3563490, 10619023, 10619022, 19733076, 21571226, 20951342, 11753655, 10471712, 19029041, 12679032, 9335506, 8548811, 21238929, 18813320, 11718263, 18024258, 8647438, 6776413, 21238930, 16872256, 10630640, 8155582, 10475061, 17258190, 16611691, 19952108, 16359857, 16339192, 7768443, 16096061, 16533943, 12361606, 18694569, 7577671, 7697720, 8670872, 19914168 | The Hedgehog (Hh) pathway was first defined by its role in segment polarity in the Drosophila melanogaster embryonic epidermis and has since been linked to many aspects of vertebrate development and disease. In humans, mutation of the Patched1 (PTCH1) gene, which encodes an inhibitor of Hh signaling, leads to tumors of the skin and pediatric brain. Despite the high level of conservation between the vertebrate and invertebrate Hh pathways, studies in Drosophila have yet to find direct evidence that ptc limits organ size. Here we report identification of Drosophila ptc in a screen for mutations that require a synergistic apoptotic block in order to drive overgrowth. Developing imaginal discs containing clones of ptc mutant cells immortalized by the concurrent loss of the Apaf-1-related killer (Ark) gene are overgrown due, in large part, to the overgrowth of wild type portions of these discs. This phenotype correlates with overexpression of the morphogen Dpp in ptc,Ark double-mutant cells, leading to elevated phosphorylation of the Dpp pathway effector Mad (p-Mad) in cells surrounding ptc,Ark mutant clones. p-Mad functions with the Hippo pathway oncoprotein Yorkie (Yki) to induce expression of the pro-growth/anti-apoptotic microRNA bantam. Accordingly, Yki activity is elevated among wild type cells surrounding ptc,Ark clones and alleles of bantam and yki dominantly suppress the enlarged-disc phenotype produced by loss of ptc. These data suggest that ptc can regulate Yki in a non-cell autonomous manner and reveal an intercellular link between the Hh and Hippo pathways that may contribute to growth-regulatory properties of the Hh pathway in development and disease. | Animals, Cell Proliferation, Drosophila, Drosophila Proteins, Female, Hedgehog Proteins, Imaginal Discs, Intracellular Signaling Peptides and Proteins, Male, MicroRNAs, Mutation, Nuclear Proteins, Phosphorylation, Protein Serine-Threonine Kinases, Receptors, Cell Surface, Trans-Activators, YAP-Signaling Proteins | null |
22,705,514 | 2012-11-05 | 2012-11-15 | 1873-2976 | Bioresource technology | Polar and aliphatic domains regulate sorption of phthalic acid esters (PAEs) to biochars. | Sun Ke, Jin Jie, Keiluweit Marco, Kleber Markus, Wang Ziying, Pan Zezhen, Xing Baoshan | eng | null | Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S. | Esters, Phthalic Acids, biochar, Charcoal, phthalic acid | IM | 22705514, S0960-8524(12)00751-1, 10.1016/j.biortech.2012.05.008 | Molecular variations among different biochar categories translate into differences in their ability to function as sorbents to three phthalic acid esters (PAEs) representing a gradient in hydrophobicity. The sorption capacity (K(OC)) for all three PAEs was the greatest for amorphous biochars (heat treatment temperature HTT=400 °C), followed by biochars produced at 300 °C, and was best explained by the hydrophobicity of the sorbate. Greater alkyl C content and higher polarity of grass chars versus wood chars prepared at similar temperatures explained both (a) the difference in sorbent strength between feedstocks and (b) the maximum in sorbent strength at relatively low HTTs (300-400 °C). Hydrophobic partitioning into 'soft' alkyl carbon and specific H-bonding involving char-bound O and N groups jointly account for high affinities of PAEs for low-HTT biochars. The results highlight the influence of feedstocks and HTTs on PAEs sorption strength and mechanism. | Adsorption, Charcoal, Esters, Phthalic Acids, Poaceae, Temperature, Wood | null |
22,705,516 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Biological denitrification with a novel biodegradable polymer as carbon source and biofilm carrier. | Wu Weizhong, Yang Feifei, Yang Luhua | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Polymers, Carbon, Nitrogen | IM | 22705516, S0960-8524(12)00690-6, 10.1016/j.biortech.2012.04.066 | A novel biodegradable polymer composed of PHBV and PLA was prepared for advanced wastewater treatment. It could serve as both biofilm carrier and carbon source for denitrification. Results of batch test showed the average denitrification rate was 0.07 mg NO(3)-N/(gh). The kinetic study demonstrated that when nitrate concentration was above 10.00 mg/L, DOC could not be detected in the effluent. In continuous packed-bed reactor, the average nitrogen removal efficiency was 94.11%. Nitrite concentration throughout the experiment was below 0.15 mg/L. The formation of NH(4)-N was observed, though small. DOC released in the effluent did not exceed 16.00 mg/L in the whole process, and it finally dropped below 1.20 mg/L. | Batch Cell Culture Techniques, Biodegradation, Environmental, Biofilms, Bioreactors, Carbon, Denitrification, Hydrogen-Ion Concentration, Kinetics, Nitrogen, Polymers, Solubility, Time Factors, Waste Disposal, Fluid | null |
22,705,515 | 2012-11-05 | 2012-11-15 | 1873-2976 | Bioresource technology | Performance and microbial community in hybrid Anaerobic Baffled Reactor-constructed wetland for nitrobenzene wastewater. | Lin Yingzi, Yin Jun, Wang Jianhui, Tian Wende | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Ribosomal, Nitrobenzenes, nitrobenzene | IM | 22705515, S0960-8524(12)00800-0, 10.1016/j.biortech.2012.05.056 | A process combining an Anaerobic Baffled Reactor (ABR) and a constructed wetland was employed to treat nitrobenzene wastewater. The overall performance was examined throughout long-term operation with a hydraulic retention time (HRT) of 24 h at 30±1 °C. The effluent nitrobenzene concentration of the ABR and constructed wetland was less than 4.81 and 1.94 mg/L, respectively, with an initial nitrobenzene concentration of 80 mg/L at the steady-state periods. The corresponding removal efficiencies were 97.02% and 73.93%, respectively. Moreover, 97.29% of aniline produced in the ABR could be removed in the subsequent wetland. The number of sequenced clones from each library was sufficient to cover archaea and eubacteria diversity at the species level and to obtain a representation of the total microbial diversity in the ABR. The predominant microbial populations in the ABR were identified as Pseudomonas putida, Methanosaeta concilii and Methanothrix soehngenii. | Anaerobiosis, Archaea, Bacteria, Biodegradation, Environmental, Bioreactors, DNA, Ribosomal, Gene Library, Nitrobenzenes, Phylogeny, Waste Disposal, Fluid, Water Purification, Wetlands | null |
22,705,517 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Optimization of liquid fermentation of microbial consortium WSD-5 followed by saccharification and acidification of wheat straw. | Wen Boting, Yuan Xufeng, Cao Yanzhuan, Liu Yan, Wang Xiaofen, Cui Zongjun | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acids, Enzymes, RNA, Ribosomal, Waste Products | IM | 22705517, S0960-8524(12)00769-9, 10.1016/j.biortech.2012.05.025 | The microbial consortium WSD-5 is composed of bacteria and fungi, and the cooperation and symbiosis of the contained microbes enhance the degradation ability of WSD-5. Experiment results showed that the highest cellulase and hemicellulase were obtained when ventilation volume was 4 L/min, stirring rate was 0 rpm, and substrate loading rate was 3%. After 6 days of cultivation, a 67.60% loss in wheat straw dry weight was observed. The crude enzyme secreted from WSD-5 after optimization was evaluated by experiments of saccharification and acidification. The maximum concentration of reducing sugars was 3254 mg/L after 48 h saccharification. The concentration of sCOD peaked on day 2 with a value of 4345 mg/L during acidification, and the biogas yield and methane yield were 22.3% and 32.3% higher than un-acidified samples. This study is the first attempt to explore both the saccharification and the acidification ability of crude enzymes secreted by microbial consortium. | Acids, Anaerobiosis, Biodegradation, Environmental, Biodiversity, Biotechnology, Carbohydrate Metabolism, Denaturing Gradient Gel Electrophoresis, Enzymes, Fermentation, Microbial Consortia, Polymerase Chain Reaction, RNA, Ribosomal, Substrate Specificity, Triticum, Waste Products | null |
22,705,518 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Catalytic pyrolysis of green algae for hydrocarbon production using H+ZSM-5 catalyst. | Thangalazhy-Gopakumar Suchithra, Adhikari Sushil, Chattanathan Shyamsundar Ayalur, Gupta Ram B | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Hydrocarbons, Oils, ZSM-5 zeolite, Zeolites | IM | 22705518, S0960-8524(12)00833-4, 10.1016/j.biortech.2012.05.080 | Microalgae are considered as an intriguing candidate for biofuel production due to their high biomass yield. Studies on bio-oil production through fast pyrolysis and upgrading to hydrocarbon fuels using algal biomass are limited as compared to other terrestrial biomass. Therefore, in this study, a fresh water green alga, Chlorella vulgaris, was taken for pyrolysis study. The average activation energy for pyrolysis zone was found to be 109.1 kJ/mol. Fixed-bed pyrolysis of algae gave a bio-oil yield of 52.7 wt.%, which accounts for 60.7 wt.% carbon yield. In addition, analytical pyrolysis of C. vulgaris was carried out in a Py/GC-MS to identify major compounds present in bio-oil with and without catalyst (H(+)ZSM-5). The study found that in catalytic-pyrolysis, as the catalyst loading increased from zero to nine times of the biomass, the carbon yield of aromatic hydrocarbons increased from 0.9 to 25.8 wt.%. | Biomass, Bioreactors, Biotechnology, Catalysis, Chlorophyta, Gas Chromatography-Mass Spectrometry, Hydrocarbons, Oils, Temperature, Thermogravimetry, Zeolites | null |
22,705,519 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | Influence of pyrolysis temperature on physicochemical properties of biochar obtained from the fast pyrolysis of pitch pine (Pinus rigida). | Kim Kwang Ho, Kim Jae-Young, Cho Tae-Su, Choi Joon Weon | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Elements, biochar, Charcoal | IM | 22705519, S0960-8524(12)00718-3, 10.1016/j.biortech.2012.04.094 | The aim of this study was to investigate the influence of pyrolysis temperature on the physicochemical properties and structure of biochar. Biochar was produced by fast pyrolysis of pitch pine (Pinus rigida) using a fluidized bed reactor at different pyrolysis temperatures (300, 400 and 500 °C). The produced biochars were characterized by elemental analysis, Brunauer-Emmett-Teller (BET) surface area, particle size distributions, field-emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, solid-state (13)C nuclear magnetic resonance (NMR) and X-ray diffraction (XRD). The yield of biochar decreased sharply from 60.7% to 14.4%, based on the oven-dried biomass weight, when the pyrolysis temperature rose from 300 °C to 500 °C. In addition, biochars were further carbonized with an increase in pyrolysis temperature and the char's remaining carbons were rearranged in stable form. The experimental results suggested that the biochar obtained at 400 and 500 °C was composed of a highly ordered aromatic carbon structure. | Biotechnology, Charcoal, Chemical Phenomena, Elements, Magnetic Resonance Spectroscopy, Particle Size, Pinus, Spectroscopy, Fourier Transform Infrared, Temperature, Thermogravimetry, X-Ray Diffraction | null |
22,705,520 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Swine wastewater treatment using a unique sequence of ion exchange membranes and bioelectrochemical system. | Lim Seung Joo, Park Wooshin, Kim Tak-Hyun, Shin In Hwan | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Cations, Membranes, Artificial, Organic Chemicals, Quaternary Ammonium Compounds, Ammonia, Nitrogen | IM | 22705520, S0960-8524(12)00764-X, 10.1016/j.biortech.2012.05.021 | An ion exchange biological reactor (IEBR) treated organic matter and nitrogen in swine wastewater at 23 °C. The enhanced IEBR enhanced the ammonium flux by electrochemical attraction. The abiotic ammonium fluxes at the applied voltage of 0, 1, and 3 V were 1.33, 1.79, and 2.73 mg/m(2)/s, respectively. In the meantime, the ammonium fluxes caused by biological nitrification at the applied voltage of 0, 1, and 3 V were 1.54, 2.07, and 3.59 mg/m(2)/s, respectively. Removal of organic matter and nitrogen in swine wastewater was proportional to the applied voltage. The average SCOD removal efficiencies at the applied voltage of 0, 1, and 2V were 59.7%, 60.2%, and 67.0%, respectively. The average total nitrogen removal efficiencies at the applied voltage of 0, 1, and 2V were 39.8%, 49.5%, and 58.7%, respectively. | Ammonia, Animals, Batch Cell Culture Techniques, Biological Oxygen Demand Analysis, Bioreactors, Cations, Electricity, Electrochemical Techniques, Hydrogen-Ion Concentration, Ion Exchange, Membranes, Artificial, Motion, Nitrification, Nitrogen, Organic Chemicals, Quaternary Ammonium Compounds, Swine, Waste Disposal, Fluid, Water Purification | null |
22,705,521 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Process investigations of extreme thermophilic fermentations for hydrogen production: effect of bubble induction and reduced pressure. | Sonnleitner Andrea, Peintner Christian, Wukovits Walter, Friedl Anton, Schnitzhofer Wolfgang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acetates, Gases, Lactic Acid, Hydrogen, Glucose | IM | 22705521, S0960-8524(12)00790-0, 10.1016/j.biortech.2012.05.046 | Hydrogen production via thermophilic dark fermentation is considered a sustainable way to produce renewable hydrogen. For industrial scale an optimisation of hydrogen production is of highest importance. The aim of this work was to evaluate induced bubble formation and applying reduced pressure as methods of removing produced hydrogen instead of external gas stripping. Evaluation was carried out in a continuously stirred tank reactor using the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus. The addition of a bubble formation inductor was able to maintain the fermentation, but only at low hydrogen production rates and yields. Applying reduced pressure at a level of 305 mbar, nitrogen stripping could be omitted and hydrogen yields of around 72% of the theoretical maximum were achieved. It was proven, that application of reduced pressure is a promising alternative to inert gas stripping to obtain high hydrogen productivities and yields for thermophilic dark fermentations. | Acetates, Anaerobiosis, Bioreactors, Biotechnology, Fermentation, Gases, Glucose, Hydrogen, Lactic Acid, Pressure, Temperature | null |
22,705,522 | 2012-11-05 | 2016-11-25 | 1873-2976 | Bioresource technology | Yeast fermentation of carboxylic acids obtained from pyrolytic aqueous phases for lipid production. | Lian Jieni, Garcia-Perez Manuel, Coates Ralph, Wu Hongwei, Chen Shulin | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Acetates, Carboxylic Acids, Fatty Acids, Formates, Lipids, Phenols, formic acid, Acetone, acetol, Acetaldehyde, glycolaldehyde | IM | 22705522, S0960-8524(12)00753-5, 10.1016/j.biortech.2012.05.010 | The presence of very reactive C1-C4 molecules adversely affects the quality bio-oils produced from the pyrolysis of lignocellulosic materials. In this paper a scheme to produce lipids with Cryptococcus curvatus from the carboxylic acids in the pyrolytic aqueous phase collected in fractional condensers is proposed. The capacities of three oleaginous yeasts C. curvatus, Rhodotorula glutinis, Lipomyces starkeyi to ferment acetate, formate, hydroxylacat-aldehyde, phenol and acetol were investigated. While acetate could be a good carbon source for lipid production, formate provides additional energy and contributes to yeast growth and lipid production as auxiliary energy resource. Acetol could slightly support yeast growth, but it inhibits lipid accumulation. Hydroxyacetaldehyde and phenols showed high yeast growth and lipid accumulation inhibition. A pyrolytic aqueous phase with 20 g/L acetate was fermented with C. curvatus, after neutralization and detoxification to produce 6.9 g/L dry biomass and 2.2 g/L lipid. | Acetaldehyde, Acetates, Acetone, Biotechnology, Carboxylic Acids, Fatty Acids, Fermentation, Formates, Hydrogen-Ion Concentration, Lipids, Phenols, Temperature, Yeasts | null |
22,705,523 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Biomass gasification and in-bed contaminants removal: performance of iron enriched olivine and bauxite in a process of steam/O2 gasification. | Barisano D, Freda C, Nanna F, Fanelli E, Villone A | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Environmental Pollutants, Iron Compounds, Magnesium Compounds, Particulate Matter, Silicates, Steam, Silicon Dioxide, olivine, Iron, Aluminum Oxide, Oxygen | IM | 22705523, S0960-8524(12)00754-7, 10.1016/j.biortech.2012.05.011 | A modified Olivine, enriched in iron content (10% Fe/Olivine), and a natural bauxite, were tested in the in-bed reduction of tar and alkali halides (NaCl and KCl) released in a process of biomass steam/O(2) gasification. The tests were carried out at an ICBFB bench scale reactor under the operating conditions of: 855-890 °C, atmospheric pressure, 0.5 steam/biomass and 0.33 ER ratios. From the use of the two materials, a reduction in the contaminant contents of the fuel gas produced was found. For the alkali halides, a decrease up to 70%(wt) was observed for the potassium concentration, while for sodium, the reduction was found to be quite poor. For the organic content, compared to unmodified Olivine, the chromatographically determined total tar quantity showed a removal efficiency of 38%(wt). Moreover, regarding the particulate content a rough doubling in the fuel gas revealed a certain brittleness of the new bed material. | Aluminum Oxide, Biomass, Bioreactors, Biotechnology, Environmental Pollutants, Gas Chromatography-Mass Spectrometry, Iron, Iron Compounds, Magnesium Compounds, Oxygen, Particulate Matter, Silicates, Silicon Dioxide, Steam | null |
22,705,524 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Hydrothermal carbonization of sugarcane bagasse via wet torrefaction in association with microwave heating. | Chen Wei-Hsin, Ye Song-Ching, Sheen Herng-Kuang | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Elements, Water, Carbon, Cellulose, bagasse | IM | 22705524, S0960-8524(12)00725-0, 10.1016/j.biortech.2012.04.101 | Hydrothermal carbonization of sugarcane bagasse using wet torrefaction is studied. The biomass is torrefied in water or dilute sulfuric acid solution and microwaves are employed to heat the solutions where the reaction temperature is fixed at 180 °C. The effects of acid concentration, heating time and solid-to-liquid ratio on the performance of wet torrefaction are investigated. It is found that the addition of sulfuric acid and increasing heating time are conducive to carbonizing bagasse. The calorific value of bagasse can be increased up to 20.3% from wet torrefaction. With the same improvement in calorific value, the temperature of wet torrefaction is lower than that of dry torrefaction around 100 °C, revealing that wet torrefaction is a promising method to upgrade biomass as fuel. The calorific value of torrefied biomass can be predicted well based on proximate, elemental or fiber analysis, and the last one gives the best estimation. | Biotechnology, Carbon, Cellulose, Elements, Heating, Microwaves, Particle Size, Saccharum, Temperature, Thermodynamics, Thermogravimetry, Water | null |
22,705,525 | 2012-11-05 | 2017-11-16 | 1873-2976 | Bioresource technology | Synthesis, characterization and swelling behavior of superabsorbent wheat straw graft copolymers. | Li Qian, Ma Zuohao, Yue Qinyan, Gao Baoyu, Li Wenhong, Xu Xing | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Polymers, Solutions, Waste Products, Hydrogel, Polyethylene Glycol Dimethacrylate, Sodium Chloride, Cellulose | IM | 22705525, S0960-8524(12)00467-1, 10.1016/j.biortech.2012.03.028 | Swelling behavior is an important characteristic for superabsorbents. A wheat straw-based superabsorbent (WS-SAB) was prepared by graft copolymerization of acrylic acid, acrylic amide and dimethyl diallyl ammonium chloride onto the cellulose of wheat straw, and its swelling and deswelling behavior was investigated. The product had a water absorbency of 133.76 g/g in distilled water and 33.83 g/g in 0.9 wt.% NaCl solution. Fourier transform infrared spectroscopy and scanning electron microscopy indicated that the monomers were successfully grafted onto the wheat straw. The largest swelling capacity was at pH 6. The effect of ions on the swelling was in the order: Na(+)>K(+)>Mg(2+)>Ca(2+) and Cl(-)>SO(4)(2-). The swelling capacity did not change after several times of water absorption and release. | Absorption, Cellulose, Hydrogel, Polyethylene Glycol Dimethacrylate, Hydrogen-Ion Concentration, Kinetics, Mechanical Phenomena, Microscopy, Electron, Scanning, Particle Size, Polymers, Sodium Chloride, Solutions, Spectroscopy, Fourier Transform Infrared, Temperature, Triticum, Waste Products | null |
22,705,526 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Maximum organic loading rate for the single-stage wet anaerobic digestion of food waste. | Nagao Norio, Tajima Nobuyuki, Kawai Minako, Niwa Chiaki, Kurosawa Norio, Matsuyama Tatsushi, Yusoff Fatimah Md, Toda Tatsuki | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Biofuels, Fatty Acids, Volatile, Organic Chemicals, Sewage, Waste Products, Water, Methane | IM | 22705526, S0960-8524(12)00789-4, 10.1016/j.biortech.2012.05.045 | Anaerobic digestion of food waste was conducted at high OLR from 3.7 to 12.9 kg-VS m(-3) day(-1) for 225 days. Periods without organic loading were arranged between the each loading period. Stable operation at an OLR of 9.2 kg-VS (15.0 kg-COD) m(-3) day(-1) was achieved with a high VS reduction (91.8%) and high methane yield (455 mL g-VS-1). The cell density increased in the periods without organic loading, and reached to 10.9×10(10) cells mL(-1) on day 187, which was around 15 times higher than that of the seed sludge. There was a significant correlation between OLR and saturated TSS in the sludge (y=17.3e(0.1679×), r(2)=0.996, P<0.05). A theoretical maximum OLR of 10.5 kg-VS (17.0 kg-COD) m(-3) day(-1) was obtained for mesophilic single-stage wet anaerobic digestion that is able to maintain a stable operation with high methane yield and VS reduction. | Anaerobiosis, Bacteria, Biofuels, Bioreactors, Biotechnology, Colony Count, Microbial, Fatty Acids, Volatile, Food, Hydrogen-Ion Concentration, Methane, Organic Chemicals, Sewage, Time Factors, Volatilization, Waste Products, Water | null |
22,705,527 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Solid-state co-digestion of expired dog food and corn stover for methane production. | Xu Fuqing, Li Yebo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Waste Products, Methane | IM | 22705527, S0960-8524(12)00726-2, 10.1016/j.biortech.2012.04.102 | Expired dog food was co-digested with corn stover for biogas production via solid-state anaerobic digestion (SS-AD) at feedstock-to-effluent (F/E) ratios of 2, 4, and 6 using effluent from a sewage sludge digester as inoculum. Degradation of the main components in dog food and corn stover was measured. Higher methane yields were obtained at lower F/E ratios and at higher percentages of dog food in the substrate. The highest methane yield of 304.4 L/kg VS(feed) was obtained for the substrate containing 50% corn stover and 50% dog food, which was an increase of 229% and 109% compared to digesting corn stover and dog food alone, respectively. Co-digestion of corn stover with dog food reduced the start-up time and volatile fatty acid (VFA) accumulation, but decreased the cellulose and xylan degradation of corn stover. | Animals, Bioreactors, Biotechnology, Dogs, Food, Methane, Time Factors, Waste Disposal, Fluid, Waste Products, Zea mays | null |
22,705,528 | 2012-11-05 | 2022-03-17 | 1873-2976 | Bioresource technology | Effect of hydrogen and carbon dioxide on carboxylic acids patterns in mixed culture fermentation. | Arslan D, Steinbusch K J J, Diels L, De Wever H, Buisman C J N, Hamelers H V M | eng | null | Journal Article | Carboxylic Acids, Propionates, Butyric Acid, Carbon Dioxide, Hydrogen, propionic acid, Acetic Acid | IM | 22705528, S0960-8524(12)00745-6, 10.1016/j.biortech.2012.05.003 | This study investigated the carboxylate spectrum from mixed culture fermentation of three organic waste streams after supplying 2 bar hydrogen and carbon dioxide or a mixture of these two gases to the headspace. Under any modified headspace, propionate production was ceased and butyrate, caproate and the total carboxylate concentrations were higher than in the reactors with N(2) headspace (control). Production of one major compound was achieved under hydrogen and carbon dioxide mixed headspace after 4 weeks of incubation. Both the highest acetate concentration (17.4 g COD/l) and the highest fraction (87%) were observed in reactors with mixed hydrogen and carbon dioxide headspace independent of the substrate used. In the control reactor, acetate made up maximum 67% of the total products. For other products, the highest concentration and fraction were seldom observed together. Selective butyrate production reaching a 75% fraction was found under the carbon dioxide headspace on the carbohydrate rich waste. | Acetic Acid, Anaerobiosis, Biological Oxygen Demand Analysis, Butyric Acid, Carbon Dioxide, Carboxylic Acids, Cell Culture Techniques, Fermentation, Hydrogen, Hydrolysis, Propionates, Time Factors, Waste Disposal, Fluid | null |
22,705,529 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Optimization of heterotrophic cultivation of Chlorella sp. for oil production. | Xie Tonghui, Sun Yuan, Du Kaifeng, Liang Bin, Cheng Rong, Zhang Yongkui | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Ribosomal, Fatty Acids, Oils | IM | 22705529, S0960-8524(12)00746-8, 10.1016/j.biortech.2012.05.004 | The oleaginous microalga Chlorella sp. LAM-H screened from freshwater was proven to be a prospective feedstock for oil production according to its fatty acid composition. In order to enhance lipid production, response surface methodology (RSM) was used with central composite design (CCD) to optimize the heterotrophic cultivation of microalgae. The experiment results showed that a satisfactory second-order polynomial regression equation was achieved with a high coefficient of determination (R(2)=0.9911) in analysis of variance. The effects of individual factors and their interactions on lipid productivity were successfully revealed. The greatest lipid productivity reached 247.16 mg l(-1) d(-1) under the optimal conditions of glucose concentration 26.2 g l(-1), sodium nitrate concentration 2.06 g l(-1) and temperature 28.18 °C. Moreover, validation tests were performed and the results were very close to the predicted values. It was demonstrated that the obtained model was effective for predicting lipid productivity of the isolated microalga. | Analysis of Variance, Cell Culture Techniques, Chlorella, DNA, Ribosomal, Fatty Acids, Heterotrophic Processes, Microalgae, Models, Biological, Molecular Sequence Data, Oils, Phylogeny, Regression Analysis, Reproducibility of Results | null |
22,705,530 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Kinetics of the bio-oxidation of volatile reduced sulphur compounds in a biotrickling filter. | Cáceres Manuel, Silva Javier, Morales Marjorie, San Martín Ricardo, Aroca Germán | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Disulfides, Sulfhydryl Compounds, Sulfides, Sulfur Compounds, methylmercaptan, dimethyl disulfide, dimethyl sulfide, Hydrogen Sulfide | IM | 22705530, S0960-8524(12)00644-X, 10.1016/j.biortech.2012.04.039 | Mixtures of volatile reduced sulphur compounds (VRSCs) like hydrogen sulphide (H(2)S), methylmercaptan (MM), dimethyl sulphide (DMS) and dimethyl disulphide (DMDS) are found in gaseous emissions of several industrial activities creating nuisance in the surroundings. Hydrogen sulphide (H(2)S) decreases the removal efficiency of volatile reduced sulphur compounds (VRSCs) in biofilters but the kinetics of this effect is still unknown. Kinetic expressions that represent the rate of bio-oxidation of H(2)S, MM, DMS and DMDS are proposed. In order to observe and quantify this effect, equimolar mixtures of MM, DMS and DMDS were fed into a biotrickling filter inoculated with Thiobacillus thioparus at different H(2)S loads. Experimental results shown a good agreement with the simulations generated by the model considering the kinetic equations proposed. The estimated kinetic constants show that H(2)S and MM have a significant inhibitory effect on the bio-oxidation of DMS and DMDS, having the H(2)S the higher effect. | Biodegradation, Environmental, Bioreactors, Computer Simulation, Disulfides, Filtration, Hydrogen Sulfide, Kinetics, Oxidation-Reduction, Regression Analysis, Sulfhydryl Compounds, Sulfides, Sulfur Compounds, Thiobacillus, Volatilization | null |
22,705,531 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Effect of cathode types on long-term performance and anode bacterial communities in microbial fuel cells. | Zhang Guodong, Wang Kun, Zhao Qingliang, Jiao Yan, Lee Duu-Jong | eng | null | Journal Article, Research Support, Non-U.S. Gov't | DNA, Ribosomal | IM | 22705531, S0960-8524(12)00758-4, 10.1016/j.biortech.2012.05.015 | Effects of cathode types on the long-term stability of microbial fuel cell (MFC) and the anodic microbial communities were studied using K(3)Fe(CN)(6) catholyte (R1), air cathode (R2) and biocathode (R3) over a testing time of 400 d. Upon 400 d of testing, the maximum power densities (P(max)) of R1 and R2 decreased by 44% and 37%, and the Coulombic efficiencies (CEs) decreased 8.4% (R1) and 2.0% (R2), respectively, using the performances on 10d as the comparison basis. Conversely, the P(max) and CE of R3 increased by 68.2% and 116.8%, respectively. The non-ohmic resistances (R(no)) in all tests were the principal contributors of cell internal resistances. Phylogenetic analyses revealed that the microbial communities on anodic surface varied with cathode types and operational history. | Bacteria, Batch Cell Culture Techniques, Bioelectric Energy Sources, DNA, Ribosomal, Denaturing Gradient Gel Electrophoresis, Electric Impedance, Electrodes, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Principal Component Analysis, Time Factors | null |
22,705,532 | 2012-11-05 | 2012-07-04 | 1873-2976 | Bioresource technology | Development of an industrial medium and a novel fed-batch strategy for high-level expression of recombinant β-mananase by Pichia pastoris. | Zheng Jia, Zhao Wei, Guo Ning, Lin Fulai, Tian Jian, Wu Lishuang, Zhou Hongbo | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Culture Media, Recombinant Proteins, beta-Mannosidase | IM | 22705532, S0960-8524(12)00809-7, 10.1016/j.biortech.2012.05.065 | An industrial medium, Corn Steep Liquor Powder Dextrose (CSD medium) was developed for constitutive expression of recombinant β-mananase by Pichia pastoris. The β-mananase activity (513 U/mL) with CSD medium was 1.64- and 2.5-fold higher than with YPD and BSM in shaken flasks. The β-mananase productivity with CSD medium was 61.0 U/mL h, which was 1.7- and 2.5-fold higher than with YPD and BSM in a 5-L fermenter based on a novel fed-batch strategy combining the real-time exponential feed mode with the DO-stat feed mode. The β-mananase activity, dry cell weight and the recombinant enzyme reached up to 5132 U/mL, 110.0 g/L and 4.50 g/L after 50 h cultivation in a 50-L fermenter. The high efficient expression of recombinant β-mananase by P. pastoris indicated that CSD medium and the novel fed-batch strategy have great potential for the production of recombinant β-mananase in industrial fermentation. | Batch Cell Culture Techniques, Biomass, Bioreactors, Culture Media, Fermentation, Hydrogen-Ion Concentration, Industrial Microbiology, Pichia, Recombinant Proteins, Time Factors, beta-Mannosidase | null |
22,705,533 | 2012-11-05 | 2013-11-21 | 1873-2976 | Bioresource technology | Redirecting the electron flow towards the nitrogenase and bidirectional Hox-hydrogenase by using specific inhibitors results in enhanced H2 production in the cyanobacterium Anabaena siamensis TISTR 8012. | Khetkorn Wanthanee, Baebprasert Wipawee, Lindblad Peter, Incharoensakdi Aran | eng | null | Journal Article, Research Support, Non-U.S. Gov't | Enzyme Inhibitors, Photosystem II Protein Complex, RNA, Messenger, Hydrogen, Hydrogenase, Nitrogenase | IM | 22705533, S0960-8524(12)00796-1, 10.1016/j.biortech.2012.05.052 | The inhibition of competitive metabolic pathways by various inhibitors in order to redirect electron flow towards nitrogenase and bidirectional Hox-hydrogenase was investigated in Anabaena siamensis TISTR 8012. Cells grown in BG11(0) supplemented with KCN, rotenone, DCMU, and DL-glyceraldehyde under light condition for 24 h showed enhanced H(2) production. Cells grown in BG11 medium showed only marginal H(2) production and its production was hardly increased by the inhibitors tested. H(2) production with either 20mM KCN or 50 μM DCMU in BG11(0) medium was 22 μmol H(2) mg chl a(-1) h(-1), threefold higher than the control. The increased H(2) production caused by inhibitors was consistent with the increase in the respective Hox-hydrogenase activities and nifD transcript levels, as well as the decrease in hupL transcript levels. The results suggested that interruption of metabolic pathways essential for growth could redirect electrons flow towards nitrogenase and bidirectional Hox-hydrogenase resulting in increased H(2) production. | Anabaena, Electrons, Enzyme Inhibitors, Gene Expression Regulation, Bacterial, Hydrogen, Hydrogenase, Light, Models, Biological, Nitrogen Fixation, Nitrogenase, Photosystem II Protein Complex, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction | null |
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